CN102487801A - Method for soilless culture of bulblets of tissue culture seedlings of oriental lily - Google Patents

Abstract

The invention discloses a method for the soilless culture of bulblets of tissue culture seedlings of oriental lily, which belongs to the technical field of flower cultivation and breeding. The method comprises the following steps: treating the tissue culture seedlings of oriental lily at 3 to 5 DEG C for 6 to 8 weeks, performing soilless culture, and culturing for two growth seasons, wherein in the first year the N-P-K-Mg mixing ratio is 80-30-40-24mg/L in the 4th to 11th weeks after planting in field, 200-60-200-48mg/L in the 12th to 24th weeks, and 0-60-400-0mg/L in the 25th to 30th weeks; and the N-P-K-Mg mixing ratio in the second year is 80-30-80-24mg/L in the 4th to 11th weeks after planting in field, 100-40-200-36mg/L in the 12th to 24th weeks, and 100-30-300-0mg/L in the 25th to 30th weeks. In the growth period, benziothiazolinone and green carbendazim are used by mixing according to a ratio of 1:1, hymexazol, zhongshengmycin and Penkang (a chemical) are used by mixing according to a ratio of 1:1:1, and the two kinds of mixtures are used alternately to prevent diseases. After the first round of culture, the average weight gain of the bulblets is 8 to 11 times and the circumference diameter increase is 3 to 4 times; and after the second round of culture, the average weight gain of the bulblets is 7 to 8 times and the circumference diameter increase is 2 to 3 times. The method has the advantages of ensuring quick growth and expansion of bulblets of lily, reducing propagation time and accelerating bulblet commercialization process, along with simple and convenient operation, and can be widely used in the breeding and cultivation of oriental lily.

Description

A kind of oriental hybrid lily tissue cultivating seedling bulbec does not have the method that earth culture is educated

Technical field:

The present invention relates to a kind of oriental hybrid lily (Oriental lily) tissue cultivating seedling kind ball does not have the method that earth culture is educated, and belongs to floriculture and breeding technical field.

Background technology:

Oriental hybrid lily is the top grade flower that receives the market favor most, and its breeding work is very important, but finds in the practice that the growth cycle of lilium oriental is longer.In the research and production practice, oriental hybrid lily will pass through tissue culture, scale cottage propagation and seminal propagation, and needing experience to cultivate more than 3 years just can become commodity kind ball.This is to plant the big obstacle that the ball commercialization is produced in oriental hybrid lily breeding, the cultivation.

Existing research major side overweights the influence to the lily cut flowers quality such as forcing culture, Different Fertilization prescription and the different cultivation matrixes etc. of commodity lily, educates with the nutrient solution prescription Study on optimized less for the no earth culture of lily bulbec.

Through the document retrieval, do not see the public reported identical with the present invention.

Summary of the invention:

The objective of the invention is to overcome the deficiency of prior art, and provide a kind of oriental hybrid lily tissue cultivating seedling bulbec not have the method that earth culture is educated, breed and new improved variety commercialization progress to accelerate lilium oriental.

Discover; The oriental hybrid lily tissue cultivating seedling is earlier through low temperature treatment; Transplant again to soilless culture substrate, use the nutrient solution fertilising of optimizing, through the cultivation of 2 seasons of growth; 8-11 times of boy's ball average weight gain, all directly 3-4 times of increase after the cultivation in 1 year, 7-8 times of bulbec average weight gain, all directly 2-3 times of increase after the cultivation in 1 year.

Oriental hybrid lily tissue cultivating seedling bulbec of the present invention does not have the method that earth culture is educated, and its concrete steps are following:

A. the oriental hybrid lily tissue cultivating seedling is handled 6-8 week under 3-5 ℃ of condition;

B. with the peat after water content 65%, the 50 times of formalin sterilizations: lily special seedling substrate sealing 3-5 days of perlite=1: 1, dried in the air 7 days after, in the cultivation case of packing into, thickness 15-20cm; Plant seedling and irrigate matrix the previous day, subsequent use; Clean the tissue cultivating seedling adhesion medium, remove the bud of growing thickly, withered and yellow blade keeps individual plant, plants in the incubator, the degree of depth is 3-5cm;

C. the seedling-slowing stage in 1-3 week was not executed nutrient solution after oriental hybrid lily was planted, but will note keeping the skin wet; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-40-24mg/L; The back all nutrient solution prescriptions of 12-24 of planting are: N-P-K-Mg=200-60-200-48mg/L, and the nutrient solution prescription in the back 25-30 week of planting is: N-P-K-Mg=0-60-400-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution; Use nutrient solution weekly 1 time, each 2L; Fertilising finishes the back and excavates lily microspecies ball 2-3 week;

D. place-1 ℃ of ventilation refrigerating chamber to preserve in the lily microspecies ball of excavating, the March in the coming year plants in incubator after taking out kind of ball routine disinfection, matrix sterilization method and fertilizing method same last year; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-80-24mg/L, and the nutrient solution prescription in the back 12-24 week of planting is: 100-40-200-36mg/L, the nutrient solution prescription in the back 25-30 week of planting is: 100-30-300-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution;

E. adopt that bacterium is upright goes out and green carbendazim is used with in 1: 1 ratio vegetative period, dislike mould spirit, middle living rhzomorph and spray health and use with in 1: 1: 1 ratio, two kinds of combined and alternativelies use, use every other month once, and be main with pouring matrix, prevent and treat disease.

The oriental hybrid lily tissue cultivating seedling is supported through the no earth cultures of above-mentioned two seasons of growth, the first round cultivate back boy's ball average weight gain 8-11 doubly, increase week footpath 3-4 doubly, second take turns cultivate back bulbec average weight gain 7-8 doubly, increase week footpath 2-3 doubly.

The present invention compares with conventional method, have the growth of easy and simple to handle, lily bulbec rapidly, expand soon, the time of breeding is shorter, quickens kind of the advantage of ball commercialization process, can be widely used in the breeding cultivation of oriental hybrid lily flowers.

The practical implementation method:

Oriental hybrid lily tissue cultivating seedling bulbec of the present invention does not have the method that earth culture is educated, and its concrete steps are following:

A. the oriental hybrid lily tissue cultivating seedling is handled 6 weeks or 7 week or 8 weeks under 3 ℃ or 5 ℃ of conditions;

B. with the peat after water content 65%, the 50 times of formalin sterilizations: the lily special seedling substrate sealing of perlite=1: 13 days or 5 days, dried in the air 7 days after, in the cultivation case of packing into, thickness 15cm or 20cm; Plant seedling and irrigate matrix the previous day, subsequent use; Clean the tissue cultivating seedling adhesion medium, remove the bud of growing thickly, withered and yellow blade keeps individual plant, plants in the incubator, the degree of depth is 3cm or 5cm;

C. the seedling-slowing stage in 1-3 week was not executed nutrient solution after oriental hybrid lily was planted, but will note keeping the skin wet; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-40-24mg/L; The back all nutrient solution prescriptions of 12-24 of planting are: N-P-K-Mg=200-60-200-48mg/L, and the nutrient solution prescription in the back 25-30 week of planting is: N-P-K-Mg=0-60-400-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution; Use nutrient solution weekly 1 time, each 2L; Fertilising finishes the back and excavates lily microspecies ball 2-3 week;

D. place-1 ℃ of ventilation refrigerating chamber to preserve in the lily microspecies ball of excavating, the March in the coming year plants in incubator after taking out kind of ball routine disinfection, matrix sterilization method and fertilizing method same last year; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-80-24mg/L, and the nutrient solution prescription in the back 12-24 week of planting is: 100-40-200-36mg/L, the nutrient solution prescription in the back 25-30 week of planting is: 100-30-300-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution;

E. adopt that bacterium is upright goes out and green carbendazim is used with in 1: 1 ratio vegetative period, dislike mould spirit, middle living rhzomorph and spray health and use with in 1: 1: 1 ratio, two kinds of combined and alternativelies use, use every other month once, and be main with pouring matrix, prevent and treat disease.

The oriental hybrid lily tissue cultivating seedling is supported through the no earth cultures of above-mentioned two seasons of growth, the first round cultivate back boy's ball average weight gain 8-11 doubly, increase week footpath 3-4 doubly, second take turns cultivate back bulbec average weight gain 7-8 doubly, increase week footpath 2-3 doubly.

The inventor is used for lilium oriental with this method and breeds.Facts have proved that this method is not only easy and simple to handle, cost is low, and breed through tissue cultivating seedling that the speed of kind of ball is fast, the commercialization time short, shortens the time in 1 year on the whole than conventional method.

Claims (1)

1. an oriental hybrid lily tissue cultivating seedling bulbec does not have the method that earth culture is educated, and it is characterized in that the concrete steps of this method are following:

A. the oriental hybrid lily tissue cultivating seedling is handled 6-8 week under 3-5 ℃ of condition;

B. with the peat after water content 65%, the 50 times of formalin sterilizations: lily special seedling substrate sealing 3-5 days of perlite=1: 1, dried in the air 7 days after, in the cultivation case of packing into, thickness 15-20cm; Plant seedling and irrigate matrix the previous day, subsequent use; Clean the tissue cultivating seedling adhesion medium, remove the bud of growing thickly, withered and yellow blade keeps individual plant, plants in the incubator, the degree of depth is 3-5cm;

C. the seedling-slowing stage in 1-3 week was not executed nutrient solution after oriental hybrid lily was planted, but will note keeping the skin wet; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-40-24mg/L; The back all nutrient solution prescriptions of 12-24 of planting are: N-P-K-Mg=200-60-200-48mg/L, and the nutrient solution prescription in the back 25-30 week of planting is: N-P-K-Mg=0-60-400-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution; Use nutrient solution weekly 1 time, each 2L; Fertilising finishes the back and excavates lily microspecies ball 2-3 week;

D. place-1 ℃ of ventilation refrigerating chamber to preserve in the lily microspecies ball of excavating, the March in the coming year plants in incubator after taking out kind of ball routine disinfection, matrix sterilization method and fertilizing method same last year; The back all nutrient solution prescriptions of 4-11 of planting are: N-P-K-Mg=80-30-80-24mg/L, and the nutrient solution prescription in the back 12-24 week of planting is: 100-40-200-36mg/L, the nutrient solution prescription in the back 25-30 week of planting is: 100-30-300-0mg/L; Increase by 1/10 times of general trace element of flowers in the above-mentioned nutrient solution;

E. adopt that bacterium is upright goes out and green carbendazim is used with in 1: 1 ratio vegetative period, dislike mould spirit, middle living rhzomorph and spray health and use with in 1: 1: 1 ratio, two kinds of combined and alternativelies use, use every other month once, and be main with pouring matrix, prevent and treat disease.