CN101642064A - Seed producing method for obtaining hybrid advantage of crassostrea gigas - Google Patents
Seed producing method for obtaining hybrid advantage of crassostrea gigas Download PDFInfo
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- CN101642064A CN101642064A CN200910015994A CN200910015994A CN101642064A CN 101642064 A CN101642064 A CN 101642064A CN 200910015994 A CN200910015994 A CN 200910015994A CN 200910015994 A CN200910015994 A CN 200910015994A CN 101642064 A CN101642064 A CN 101642064A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to a seed producing method for obtaining the hybrid advantage of a crassostrea gigas, which is characterized by comprising the following steps: selecting Chinese and Korean crassostrea gigases as parent oysters to carry out temporary culture; ripening the Chinese and Korean crassostrea gigases synchronously; then hybridizing sperms and ovums of the Chinese and Korean crassostrea gigases by artificial insemination; and finally carrying out the incubation, the seed culture and the growth of the hybridized oyster. The invention cultures a crassostrea gigas hybridized seed with high disease resistance and survival rate and rapid growing speed by hybridizing two crassostrea gigas geographic populations with reproductive isolation; the production performance of the crassostrea gigas hybridized seed is obviously better than that of a Chinese locally inbred comparison population; and the method is simple and has favorable industrialized application prospect.
Description
Technical field
The present invention relates to the preparation method of a kind of long oyster (Crassostrea gigas) intraspecific hybridization new lines, be particularly related to the different geographical populations of the long oyster of a kind of utilization and hybridize the heterotic producing method for seed of the long oyster of acquisition, belong to marine organisms new variety selective breeding technology field.
Background technology
Long oyster also claims Pacific oyster, belongs to Mollusca (Mollusca), lamellibranchiata (Lamellibranchia), wing subclass (Pterimorphia), pearl shell order (Pterioida), Ostreidae (Ostridea).Long oyster is important economy kind because its nutritive value is higher, owing to the breakthrough of oyster indoor industrially artificial breeding production technology, originates for oyster culture development already provides sufficient seed again.Therefore, it is coastal to spread all over north and south in China head's oyster culture zone.Expansion along with the scale of breed, output increases substantially, the quality of long oyster is but descending, degenerating also appears in germplasm, show the shortcoming that long oyster dressing percentage is low, form is irregular, meat is bad of breed, therefore, the market price is on the low side, do not reach oyster live body export standard, have a strong impact on the development of oyster culture industry.Therefore, how to obtain to grow fast, economic characters are better, resistance against diseases is strong, the improved seeds of good stress resistance become and realize growing that oyster culture is already increased production, the key of synergy.
Hybridization is one of important channel of aquatic biological breeding, has brought into play great function in the breed improvement of aquatic biological with in producing.No matter the Chinese population and the reciprocal hybrid generation between Japanese population of chlamys farreri are to grow in early days, and still growing in mid-term all shows tangible hybrid vigour; Chlamys farreri China population and Russian population filial generation also show hybrid vigour.In addition, selection cross also has research in Bao, pteria martensii and other marine cultured animal, and has obtained certain achievement.But long at present oyster does not also have the population intermolecular hybrid to obtain heterotic report.
Summary of the invention
The purpose of this invention is to provide long oyster of a kind of utilization China and the hybridization of Korea S head oyster and obtain the heterotic producing method for seed of long oyster, thereby cultivate the survival rate height, the heterosis, hybrid vigor of growing fast grows oyster significantly.
The heterotic producing method for seed of the long oyster of a kind of acquisition, it is characterized in that selecting the long oyster of China and Korea S as close shellfish, carry out supporting temporarily, synchronous accelerating China and Korea S head oyster, utilize artificial insemination that China and essence, the ovum of Korea S head oyster are hybridized then, hybridize hatching, the seed rearing of oyster at last and form.
Two long oyster geographical populations that the present invention utilizes reproduction to isolate are hybridized, cultivate that premunition is strong, survival rate is high, the long oyster hybrid seedlings of fast growth, this length oyster hybrid seedlings production traits obviously is better than the control population of Chinese local selfing, and method is simple, has good commercial application prospect.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail.
1. select close shellfish to carry out supporting temporarily
The used China head oyster colony of the present invention takes from the Rushan, Shandong in February, 2008, Korea S head oyster colony takes from Busan, Korea in January, 2008.Select individual big, the shell mould rule, healthy individuality goes into indoorly in 5-10 ℃ of seawater behind the pond to support temporarily.
2. synchronous accelerating
With the above-mentioned indoor long oyster 1-2 of falling pond every day that supports temporarily, the diatom of throwing something and feeding, flat algae and starch bait, 0.5-1 ℃ of day intensification, rise to 17-18 ℃ after, the maintenance water temperature is constant.
3. utilize artificial insemination to hybridize
Long oyster behind the above-mentioned accelerating is dissected, got a little gonadal tissue, being placed on to drip has on the slide of seawater, and what be that graininess scatters is ovum, and what be that smoke-like fills the air out is sperm.The ovum and the sperm that obtain are respectively hybridized in the mode of mass mating.
Experimental group:
Control group:
(annotate: " ♀ " expression is female,
Represent male)
4. hatching
Hybridization back fertilized egg density is 30-50/ml, and water temperature is controlled at 22-24 ℃, and inflation is stirred, and hatches.
The hatching after seed rearing with form
When D shape larva is arrived in embryonic development, with floatation D shape larva is moved into nursery pond and cultivate, larval density is 5-10/ml, and water temperature is controlled at 22-24 ℃, inflation.Open-mouthed bait is a chrysophyceae, mixes throw something and feed flat algae, Chaetoceros afterwards, and day throws something and feeds 3-4 time, and feeding volume is full with stomach to be standard.Day is changed water 1-2 time, quantity of exchanged water 50%.When eyespot appears in the larva of 30-40%, throw in adherance.After throwing in adherance, day change water 1 time, quantity of exchanged water 70-80%, the chrysophyceae of throwing something and feeding, flat algae and Chaetoceros, feeding volume is determined according to residual bait situation in the situation of ingesting of juvenile mollusk and the water.In indoor continuous culture, after the affirmation natural waters does not have wild oyster floating larvae, go to marine cage buoyant raft and hang breed.The breeding density and the water depth of each experimental group all are consistent.Between mature stage, cage is fetched, rejected aufwuch on the cage every 2 months, the observation growing state, and evacuate density gradually.
The present invention has added up the fertilization rate and the incubation rate (table 1) of each experimental group in specific implementation process, and grows seedlings and the growth and the blue or green condition of surviving of each experimental group of mature stage.
With
Two hybridization groups fertilization rate between
With
Between two control groups,
Fertilization rate and incubation rate be higher than other three mating groups.
In larva and juvenile mollusk phase, the long oyster ♂ of Korea S head oyster ♀ * China and the long oyster ♀ of the China * shell height of Korea S head oyster ♂ two hybridization groups, shell length showed the obvious growth advantage from the 10th day.The high heterosis rate of two hybridization group shells: the 180th day be 9.76 and 5.85, the 300 days be 8.42 and 3.52 (table 2).
In sum, the mating between long oyster China colony and the Korea S colony can produce the hybrid seedlings with good production traits, has higher fertilization rate and survival rate, and growth rate is significantly higher than control group, has very significantly hybrid vigour.This producing method for seed is easy, has stronger operability, is convenient to directly promote realize commercial application.
The fertilization rate of each experimental group of table 1 and incubation rate
Annotate: the long oyster of K representative Korea S population; The long oyster of C representative China population; Represent significant difference (P＜0.05) with different alphabetic flags in the delegation
The heterosis rate of table 2 hybridization group
Annotate: the long oyster of K representative Korea S population; The long oyster of C representative China population
Claims (2)
1. one kind obtains the heterotic producing method for seed of long oyster, it is characterized in that selecting the long oyster of China and Korea S as close shellfish, carry out supporting temporarily, synchronous accelerating China and Korea S head oyster, utilize artificial insemination that China and essence, the ovum of Korea S head oyster are hybridized then, hybridize hatching, the seed rearing of oyster at last and form.
2. according to the described producing method for seed of claim 1, it is characterized in that described hybridization refers to hybridize in the mode of mass mating.
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| CN200910015994A CN101642064A (en) | 2009-06-10 | 2009-06-10 | Seed producing method for obtaining hybrid advantage of crassostrea gigas |
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| CN200910015994A CN101642064A (en) | 2009-06-10 | 2009-06-10 | Seed producing method for obtaining hybrid advantage of crassostrea gigas |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990854A (en) * | 2010-10-29 | 2011-03-30 | 中国海洋大学 | Breeding method of pure color breeding line of pacific oyster shells |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN103004648A (en) * | 2011-09-22 | 2013-04-03 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| WO2013123708A1 (en) * | 2012-02-23 | 2013-08-29 | 中国海洋大学 | Method for sampling crassostrea gigas non-destructively |
| CN103719000A (en) * | 2013-12-20 | 2014-04-16 | 中国科学院南海海洋研究所 | Fertility recovery method for oyster distant hybrid sterility |
| CN103749357A (en) * | 2014-01-08 | 2014-04-30 | 中国科学院南海海洋研究所 | Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker |
| CN103814867A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Cultivation method of new hybrid and backcross crassostrea gigas strain |
| CN103814846A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Species preparation method for oyster inter-species nucleoplasm interchanged new line |
| CN103814848A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Breeding method for new line of hybrid Crassostrea hongkongensis and Crassostrea gigas |
| CN103814810A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Method of obtaining oyster distant intergeneric hybrid |
| CN103907529A (en) * | 2013-12-18 | 2014-07-09 | 中国科学院南海海洋研究所 | Breeding method capable of fixing oyster distant hybrid backcross advantages |
| CN107637551A (en) * | 2017-11-15 | 2018-01-30 | 中国海洋大学 | A kind of breeding method of the low temperature resistant Kumamoto oyster new lines in the north |
| CN115943913A (en) * | 2022-03-15 | 2023-04-11 | 中国海洋大学 | Breeding method for rapid-growth strain of crassostrea gigas |
-
2009
- 2009-06-10 CN CN200910015994A patent/CN101642064A/en active Pending
Cited By (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101990854A (en) * | 2010-10-29 | 2011-03-30 | 中国海洋大学 | Breeding method of pure color breeding line of pacific oyster shells |
| CN103004648A (en) * | 2011-09-22 | 2013-04-03 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| CN103004648B (en) * | 2011-09-22 | 2014-09-17 | 汕头大学 | Breeding method for improving DHA (Docosahexaenioc Acid) content in oyster body |
| WO2013123708A1 (en) * | 2012-02-23 | 2013-08-29 | 中国海洋大学 | Method for sampling crassostrea gigas non-destructively |
| CN102742532A (en) * | 2012-07-31 | 2012-10-24 | 中国海洋大学 | Cultivation method of novel strain of crassostrea gigas with pure purple left shells |
| CN102763617A (en) * | 2012-08-20 | 2012-11-07 | 中国海洋大学 | Artificial breeding method of sikamea oysters |
| CN102939916A (en) * | 2012-10-19 | 2013-02-27 | 汕头大学 | EPA content-increasing oyster breeding method and application thereof |
| CN103907529A (en) * | 2013-12-18 | 2014-07-09 | 中国科学院南海海洋研究所 | Breeding method capable of fixing oyster distant hybrid backcross advantages |
| CN103907529B (en) * | 2013-12-18 | 2016-05-11 | 中国科学院南海海洋研究所 | The backcross breeding method of advantage of a kind of fixing oyster distant hybrid |
| CN103719000A (en) * | 2013-12-20 | 2014-04-16 | 中国科学院南海海洋研究所 | Fertility recovery method for oyster distant hybrid sterility |
| CN103719000B (en) * | 2013-12-20 | 2015-09-02 | 中国科学院南海海洋研究所 | The fertility restorer method that one seed oyster distant hybrid is sterile |
| CN103749357A (en) * | 2014-01-08 | 2014-04-30 | 中国科学院南海海洋研究所 | Method of producing seeds of Crassostrea hongkongensis by using gill aperture freeness as marker |
| CN103814867A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Cultivation method of new hybrid and backcross crassostrea gigas strain |
| CN103814846A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Species preparation method for oyster inter-species nucleoplasm interchanged new line |
| CN103814848A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Breeding method for new line of hybrid Crassostrea hongkongensis and Crassostrea gigas |
| CN103814810A (en) * | 2014-02-28 | 2014-05-28 | 中国科学院南海海洋研究所 | Method of obtaining oyster distant intergeneric hybrid |
| CN107637551A (en) * | 2017-11-15 | 2018-01-30 | 中国海洋大学 | A kind of breeding method of the low temperature resistant Kumamoto oyster new lines in the north |
| CN107637551B (en) * | 2017-11-15 | 2020-09-29 | 中国海洋大学 | Method for breeding new strain of northern low-temperature-resistant crassostrea sikamea |
| CN115943913A (en) * | 2022-03-15 | 2023-04-11 | 中国海洋大学 | Breeding method for rapid-growth strain of crassostrea gigas |
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Application publication date: 20100210 |