AU2021104593A4 - Cultivation method of first-generation commercial seed hybrids of female Patinopecten caurinus and male Patinopecten yessoensis - Google Patents
Cultivation method of first-generation commercial seed hybrids of female Patinopecten caurinus and male Patinopecten yessoensis Download PDFInfo
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- 241001161425 Patinopecten caurinus Species 0.000 title claims abstract description 68
- 241000237516 Mizuhopecten yessoensis Species 0.000 title claims abstract description 60
- 238000012364 cultivation method Methods 0.000 title description 2
- 235000020637 scallop Nutrition 0.000 claims abstract description 71
- 241000237503 Pectinidae Species 0.000 claims abstract description 34
- 238000004519 manufacturing process Methods 0.000 claims abstract description 28
- 235000013601 eggs Nutrition 0.000 claims abstract description 24
- 230000004083 survival effect Effects 0.000 claims abstract description 12
- 238000000034 method Methods 0.000 claims abstract description 9
- 241000237509 Patinopecten sp. Species 0.000 claims description 37
- 230000003203 everyday effect Effects 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- 238000003306 harvesting Methods 0.000 claims description 6
- 230000000638 stimulation Effects 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 210000002149 gonad Anatomy 0.000 claims description 4
- 239000013535 sea water Substances 0.000 claims description 4
- 238000007796 conventional method Methods 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 16
- 230000001488 breeding effect Effects 0.000 abstract description 11
- 238000009396 hybridization Methods 0.000 abstract description 6
- 230000001568 sexual effect Effects 0.000 abstract description 4
- 239000003643 water by type Substances 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 241000195493 Cryptophyta Species 0.000 description 6
- 241000237517 Patinopecten Species 0.000 description 6
- 210000004508 polar body Anatomy 0.000 description 6
- 229920001661 Chitosan Polymers 0.000 description 5
- 241000206761 Bacillariophyta Species 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 241001232812 Caurinus Species 0.000 description 2
- 210000000349 chromosome Anatomy 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000004720 fertilization Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 206010020880 Hypertrophy Diseases 0.000 description 1
- 229930194936 Tylosin Natural products 0.000 description 1
- 239000004182 Tylosin Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- WBPYTXDJUQJLPQ-VMXQISHHSA-N tylosin Chemical compound O([C@@H]1[C@@H](C)O[C@H]([C@@H]([C@H]1N(C)C)O)O[C@@H]1[C@@H](C)[C@H](O)CC(=O)O[C@@H]([C@H](/C=C(\C)/C=C/C(=O)[C@H](C)C[C@@H]1CC=O)CO[C@H]1[C@@H]([C@H](OC)[C@H](O)[C@@H](C)O1)OC)CC)[C@H]1C[C@@](C)(O)[C@@H](O)[C@H](C)O1 WBPYTXDJUQJLPQ-VMXQISHHSA-N 0.000 description 1
- 229960004059 tylosin Drugs 0.000 description 1
- 235000019375 tylosin Nutrition 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Marine Sciences & Fisheries (AREA)
- Fodder In General (AREA)
Abstract
A method for breeding commercial seedlings of female Patinopecten caurinus and male
Patinopecten yessoensis hybrids. It is characterized by the establishment of multiple semi
sibling populations of female Patinopecten caurinus and male Patinopecten yessoensis and
their own self-reproductive populations; Select the best hybrid population, and the
corresponding parental self-reproduction populations will become the female Patinopecten
caurinus species production population and the male Patinopecten yessoensis species
production population that can be used for commercial hybridization; After the scallops reach
sexual maturity, the eggs of the female Patinopecten caurinus species production population
and the sperm of the male Patinopecten yessoensis species production population are used to
produce a hybrid first-generation commercial seed. The obtained hybrid first-generation
commercial seedlings have the advantages of two scallops at the same time, with fast growth,
wide adaptability and strong stress resistance, and are suitable for breeding in the northern
waters of China. The yield is more than 20% higher than that of the Patinopecten yessoensis,
and the survival rate is increased by about 10%. It is expected to be widely promoted in the
future.
Description
Cultivation method of first-generation commercial seed hybrids of female
Patinopecten caurinus and male Patinopecten yessoensis
The invention belongs to shellfish breeding technology, and particularly relates to
a breeding method for producing hybrid first-generation commercial seedlings by using
female Patinopecten caurinus and male Patinopecten yessoensis.
Patinopecten caurinus is a large cold water scallop native to the waters from
Alaska to California in the northeastern Pacific Ocean. It has high heterozygosity, rich
genetic diversity, narrow temperature adaptation range, maximum shell length up to
cm, large shell width, hypertrophy of the adductor muscle, high meat yield, and
delicious taste. Therefore, processed Patinopecten caurinus products are very popular
in Europe and the United States, and are recognized as one of the finest breeding species
in the world.
The Patinopecten yessoensis is native to Japan, Russia, and North Korea. Since it
was introduced to China by the Liaoning Institute of Marine Fisheries in 1982, it has
been artificially cultured on a large scale in Shandong and Liaoning, with large
individuals, fatty shell columns, delicate flesh, delicious taste, rich nutrition and high
market value. However, its slow growth rate, long growth cycle, and due to repeated
generations of inbreeding, resulting in frequent disease, germplasm degradation, high
mortality and other problems highlighted. It has brought significant losses to the
aquaculture industry and seriously restricted the healthy and sustainable development of scallop farming in China. In order to solve the above problems, genetic improvement of germplasm of scallops is imperative.
It was found that Alaska scallops and scallops belong to the same genus
Patinopecten and have the same chromosome number. And the two scallops have
complementary traits in terms of individual size, growth and temperature adaptability,
so it is possible to interbreed Patinopecten caurinus with scallops. One of the most
effective methods is cross-breeding to produce a hybrid scallop with large individuals,
fast growth and resistance to adversity.
The purpose of the present invention is to provide a method for breeding female
Patinopecten caurinus and Patinopecten yessoensis hybrid generation commercial
seeds, in order to solve the existing problems encountered in Patinopecten caurinus
nursery and breeding.
The invention is based on the following ideas: 1) Alaska scallops and scallops
belong to the genus Patinopecten and have the same number of chromosomes, thus
making it possible to successfully interbreed between the two scallops. (2) The two
scallops have complementary traits in terms of individual size, growth and temperature
adaptability, so if the two scallops are interspecies crossed, it is possible to produce a
hybrid scallop with large size, fast growth, wide range of temperature adaptability and
high resistance to adversity that combines the good genes of the two scallops. 3)
Through interspecies hybridization, use the excellent germplasm resources of Alaska
scallop to improve the germplasm of Patinopecten yessoensis, that is, through the establishment of female Patinopecten caurinus and male Patinopecten yessoensis matching lines separately, the high combining ability of female Patinopecten caurinus and male Patinopecten yessoensis hybrid matching lines are cultivated, and the heterosis of the first hybrid is directly used.
The technical scheme of the present invention:
Establishment of female Patinopecten caurinus production groups: 25 male and 25
female mature Patinopecten caurinus and 25 male Patinopecten yessoensis were
selected, respectively. Use the conventional method of dry and temperature stimulation
to make each female Patinopecten caurinus lay eggs in a separate container and add 10
6 chitosan for 20 minutes. Obtain the eggs of more than 10 Patinopecten caurinus, and
combine 25 male Patinopecten yessoensis or Patinopecten caurinus to produce sperm.
Divide the eggs of each spawning Patinopecten caurinus into two parts and fertilize
them with sperm from Patinopecten caurinus or Patinopecten yessoensis respectively.
Establish more than 10 female Patinopecten caurinus x Patinopecten yessoensis hybrid
populations and corresponding more than 10 Patinopecten caurinus self-breeding
populations. All populations were raised under the same conditions and the best hybrid
population was selected based on the overall performance of traits such as growth and
survival at harvest. The corresponding selfed population of Alaska scallop becomes a
production population of female Patinopecten caurinus species that can be used for
commercial hybridization.
The establishment of the production group of male Patinopecten yessoensis: 25
mature female and 25 male Patinopecten yessoensis and 25 female Patinopecten caurinus were selected respectively. Using the conventional method of dry in the shade and temperature stimulation, each male Ezo scallop was refined in a separate container, and the sperm of 10 Patinopecten yessoensis was obtained. Combine 25 female
Patinopecten caurinus to lay eggs and add 10 -6 chitosan for 20 minutes, mix and divide
into 10 portions. The sperm of 10 Patinopecten yessoensis were respectively fertilized
to establish a hybrid population of 10 female Patinopecten caurinus x male
Patinopecten yessoensis; Combine 25 female Patinopecten yessoensis to lay their eggs,
mix them and divide them into 10 parts, respectively fertilize them with the sperm of
Patinopecten yessoensis, and establish 10 corresponding selfed populations of
Patinopecten yessoensis; All the populations are bred under the same conditions, and
the best hybrid population is selected according to the overall performance of growth
and survival at harvest, and the corresponding selfed population of the Patinopecten
yessoensis becomes a male Patinopecten yessoensis species that can be used for
commercial hybrid production group.
The female Patinopecten caurinus in the female Patinopecten caurinus species
production population are induced to spawn and the eggs are then fertilized with the
sperm of the male Patinopecten yessoensis in the male Patinopecten caurinus species
production population, the hybrid first-generation scallop seed with significant growth
advantage can be obtained. At the same time, the female Patinopecten caurinus species
production group and the Patinopecten yessoensis species production group were self
propagated to obtain a male and female scallop production group that can be used for
the next year's hybrid generation.
The hybrid first-generation commercial seed obtained by the present invention has
the excellent characteristics of two scallops at the same time, with fast growth, wide
adaptability and strong stress resistance. It is suitable for breeding in the northern waters
of China, such as Dalian Changhai and Shandong Changdao. The output is 20% higher
than the existing Patinopecten yessoensis, and the survival rate is increased by about
Example 1
The specific operation steps of the present invention are as follows:
1) Establishment of female Patinopecten caurinus production groups: 50 male and
female Patinopecten caurinus and 50 male Patinopecten yessoensis were selected
respectively. In the nursery room at the beginning of February, the males and females
of the two scallops were cultivated separately at a stocking density of 10 scallops/m3
and fed 6 times a day with monocellular algae such as diatoms and flat algae with a
concentration of 2 to 3 million cells/ml , feed 15L/m 3 each time, change one-half of the
water every day, pour the pool every six days, and continuously inflate with an air
compressor. After entering the pond, the temperature is increased by 0.5°C every day,
and the temperature is kept at 8°C for several days, and the feeding amount of bait is
gradually increased until the scallops reach sexual maturity. 25 male and 25 female
Patinopecten caurinus and 25 male Patinopecten yessoensis with well-developed
gonads were selected and stimulated for 1h by drying in the shade. Single female Alaska
scallops were then placed in 25L plastic buckets pre-filled with 12°C seawater to obtain eggs from at least 10 Alaska scallops, respectively. After spawning, the eggs were placed under a microscope for 20 minutes with the addition of 10-6 chitosan to check for sperm contamination, and discarded if polar bodies were released which indicated fertilization. If no polar bodies were released, they were collected; 25 male
Patinopecten caurinus or Patinopecten yessoensis were combined separately to produce
sperm. The eggs of each spawning Patinopecten caurinus were divided into two parts,
and sperm of ezo scallop or Patinopecten caurinus were added to each. The amount of
sperm should be 4-5 sperm around each egg on microscopic examination, if not enough
can continue to add. 10 female Patinopecten caurinus x male Ezo scallop hybrid
populations and corresponding Patinopecten caurinus selfed populations were
established respectively. Fertilized eggs are incubated in 1OOL white plastic drums, and
larvae are reared, intermediate fry are reared and raised at sea according to conventional
Alaska scallop breeding methods. The best hybrid population was selected based on the
overall performance of the hybrid population in terms of growth and survival traits at
harvest, and the corresponding Alaska scallop self-fertility population became the
female Alaska scallop breeding population available for commercial hybrid production.
The establishment of the production group of male Patinopecten yessoensis: 50
male and 50 female Patinopecten yessoensis and 50 female Patinopecten caurinus were
selected respectively. In the nursery room at the beginning of February, the males and
females of the two scallops were cultivated separately at a stocking density of 10
scallops/m 3 and fed 6 times a day with monocellular algae such as diatoms and flat
algae with a concentration of 2 to 3 million cells/ml , feed 15L/m 3 each time, change one-half of the water every day, pour the pool every six days, and continuously inflate with an air compressor. After entering the pond, the temperature is increased by 0.5°C every day, and the temperature is kept at 8°C for several days, and the feeding amount of bait is gradually increased until the scallops reach sexual maturity. 25 male and 25 female Patinopecten yessoensis and 25 female Patinopecten caurinus with mature gonads were selected respectively. After 1 h of drying in the shade and stimulation, single male scallops were placed in a 25 L plastic bucket pre-filled with 12°C seawater to obtain sperm from each of the 10 Patinopecten yessoensis. 25 female Patinopecten yessoensis or Patinopecten caurinus were spawned together separately, and after spawning, 10 -6 dibutramine was added and left for 20 minutes. Check under the microscope for sperm contamination. If there is a polar body released, it means that it has been fertilized, then discard it, if there is no polar body released, then collect. Divide the eggs of each type of scallop into 10 parts, and add 10 sperms from Patinopecten yessoensis respectively. The amount of sperm should be microscopically inspected for
4-5 sperms around each egg, if it is not enough, you can continue to add it. 10 female
Patinopecten caurinus x male Ezo scallop hybrid populations and 10 corresponding Ezo
scallop selfed populations were established respectively. The fertilized eggs are
incubated in 1OOL white plastic buckets, and the larvae are bred, intermediate fry are
bred and raised at sea according to the conventional Ezo scallop breeding method.
Selecting the best hybrid population based on the overall performance of traits such as
growth and survival at harvest. The corresponding self-fertilized population of
Patinopecten yessoensis became a production population of male Ezo scallop breeder
shells that could be used for commercial hybrids.
Cultivation of commercial seedlings of the hybrid generation: select excellent
female Patinopecten caurinus and male Patinopecten yessoensis from the selected
female Patinopecten caurinus production groups and male ezo scallop production
groups, with a ratio of male to female of 3:1. In February, it was incorporated into the
nursery room to promote maturity. After the parent shells matured, the eggs and sperm
were laid separately according to the conventional spawning method, after the Alaska
scallop spawned in large quantities, a small amount of sperm from the scallop was
added and stirred, so that 4-5 sperm around each egg is appropriate. After normal
hatching, the hybrid generation of commercial seeds is obtained by cultivating the
larvae of Patinopecten yessoensis and intermediate seeds according to the conventional
method of cultivation.
The reproduction of the breeding population for hybridization: more than 200 male
and female individuals were selected from the female Patinopecten caurinus production
population according to the male to female ratio of 3:1. Following conventional Alaska
scallop nursery, holding and rearing methods, a production population of female Alaska
scallop seeds is obtained that can be used for hybridization the following year. The
same method was used to obtain a production population of Patinopecten yessoensis
breeder shells that could be used for hybridization the following year.
The present invention provides a nutritional treatment with appropriate
concentrations of tylosin added after the first year of Patinopecten caurinus spawning
to improve fertilization and hatching rates.
Example 2
5 mature female Patinopecten caurinus and 5 male Patinopecten yessoensis were
taken at the end of February 2016. Alaska scallop eggs and ezo scallop sperm were
obtained after warming and drying in the shade to stimulate them respectively.
Patinopecten caurinus eggs are treated with 10 -6 chitosan for 20 minutes and then
insemated with the sperm of Patinopecten yessoensis. Obtained 3 female Patinopecten
caurinus x male Patinopecten yessoensis hybrid generation family.
As measured on December 17, 2016, the average shell height of the optimal hybrid
family was 42.01.6 mm, the shell length was 40.1+1.7 mm, the shell width was
9.0+0.6 mm, the weight was 6.52+0.90 grams, and the survival rate during the growing
period was 95.8% ; During the same period, the average shell height of the
Patinopecten yessoensis control group was 38.0 2.7 mm, the shell length was 36.3+
2.6 mm, the shell width was 7.9 0.8 mm, and the body weight was 4.64 1.03 mm.
The grow-out survival rate was 88.6%. Compared with Patinopecten yessoensis, the
shell height of the hybrid family increased by about 10.53%, the shell length increased
by about 10.47%, the shell width increased by about 13.92%, the weight increased by
about 40.52%, and the survival rate increased by about 8.13%.
It can be seen that the growth rate and survival rate of the hybrid offspring of the
interspecies hybrid breeding of female Patinopecten caurinus and male Patinopecten yessoensis have been significantly improved, which proves that this hybrid has significant breeding value and is expected to be widely promoted; the establishment of the production group of male Patinopecten yessoensis: 50 male and female Patinopecten yessoensis and 50 female Patinopecten caurinus were selected respectively; in the nursery room at the beginning of February, the males and females of the two scallops were cultivated separately at a stocking density of 10 scallops/m 3 and fed 6 times a day with monocellular algae such as diatoms and flat algae with a concentration of 2 to 3 million cells/ml , feed 15L/m 3 each time, change one-half of the water every day, pour the pool every six days, and continuously inflate with an air compressor; after entering the pond, the temperature is increased by 0.5°C every day, and the temperature is kept at 8°C for several days, and the feeding amount of bait is gradually increased until the scallops reach sexual maturity. 25 male and 25 female Patinopecten yessoensis and 25 female Patinopecten caurinus with mature gonads were selected respectively; after 1 h of drying in the shade and stimulation, single male scallops were placed in a 25 L plastic bucket pre-filled with 12°C seawater to obtain sperm from each of the 10 Patinopecten yessoensis; 25 female Patinopecten yessoensis or Patinopecten caurinus were spawned together separately, and after spawning, 10-6 chitosan was added and left for 20 minutes; check under the microscope for sperm contamination. If there is a polar body released, it means that it has been fertilized, then discard it, if there is no polar body released, then collect; divide the eggs of each type of scallop into 10 parts, and add 10 sperms from Patinopecten yessoensis respectively; the amount of sperm should be microscopically inspected for 4-5 sperms
I1
around each egg, if it is not enough, you can continue to add it; 10 female Patinopecten
caurinus x male Ezo scallop hybrid populations and 10 corresponding Ezo scallop
selfed populations were established respectively; the fertilized eggs are incubated in
1OOL white plastic buckets, and the larvae are bred, intermediate fry are bred and raised
at sea according to the conventional Ezo scallop breeding method; selecting the best
hybrid population based on the overall performance of traits such as growth and
survival at harvest; the corresponding self-fertilized population of Patinopecten
yessoensis became a production population of male Ezo scallop breeder shells that
could be used for commercial hybrids;
Claims (2)
1. A method for observing seedlings of a hybrid of female Patinopecten caurinus
and male Patinopecten yessoensis, it is characterized by the observation of 25 mature
male and female Patinopecten caurinus and 25 male Patinopecten yessoensis
respectively in the first year, and the observation of each female Patinopecten caurinus
in a separate container by the conventional method of drying in the shade with
temperature stimulation,.all populations were observed under the same conditions and
the best hybrid population was observed based on the overall performance of growth
and survival traits at harvest, the corresponding Alaska scallop self-fertility population
becomes the female Alaska scallop observation shell production population for
commercial hybrids; in the first year, 25 male and 25 female Alaska scallops and 25
female scallops with mature age were also observed at the same time, obtaining sperm
from 10 scallops, 25 female Patinopecten caurinus were observed.
2. A method for observing female Patinopecten caurinus and male Ezo scallop
hybrid generation commercial seedlings, characterized by:
establishment of female Patinopecten caurinus production groups: 50 male and
female Patinopecten caurinus and 50 male Patinopecten yessoensis were observed
respectively, in the nursery room at the beginning of February, the males and females
of the two scallops were observed separately at a stocking density of 10 scallops/m3
and change one-half of the water every day, pour the pool every six days, and
continuously inflate with an air compressor; after entering the pond, the temperature is
increased by 0.5°C every day, and the temperature is kept at 8°C for several days; 25 male and 25 female Patinopecten caurinus and 25 male Patinopecten yessoensis with well-developed gonads were observed for lh by drying in the shade; single female
Alaska scallops were then placed in 25L plastic buckets pre-filled with 12°C seawater
to observe eggs from at least 10 Alaska scallops, respectively; 25 male Patinopecten
caurinus or Patinopecten yessoensis were observed separately to produce sperm; the
eggs of each spawning Patinopecten caurinus were observed into two parts, and sperm
of ezo scallop or Patinopecten caurinus were observed in each; 10 female Patinopecten
caurinus x male Ezo scallop hybrid populations and corresponding Patinopecten
caurinus selfed populations were observed.
observation of commercial seedlings of the hybrid generation: observed excellent
female Patinopecten caurinus and male Patinopecten yessoensis from the observed
female Patinopecten caurinus production groups and male ezo scallop production
groups
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462310A (en) * | 2022-10-11 | 2022-12-13 | 广西南亚热带农业科学研究所 | Method for crossbreeding radix puerariae |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
-
2021
- 2021-07-27 AU AU2021104593A patent/AU2021104593A4/en not_active Ceased
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115462310A (en) * | 2022-10-11 | 2022-12-13 | 广西南亚热带农业科学研究所 | Method for crossbreeding radix puerariae |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
| CN116391654B (en) * | 2023-03-30 | 2023-11-03 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
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