CN108633799B - First-filial generation cultivation method for female patinopecten yessoensis and male alaska scallop - Google Patents
First-filial generation cultivation method for female patinopecten yessoensis and male alaska scallop Download PDFInfo
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- 235000020637 scallop Nutrition 0.000 title claims abstract description 142
- 241000237516 Mizuhopecten yessoensis Species 0.000 title claims abstract description 101
- 241000237509 Patinopecten sp. Species 0.000 title claims abstract description 97
- 238000012364 cultivation method Methods 0.000 title claims abstract description 9
- 241000237503 Pectinidae Species 0.000 claims abstract description 48
- 238000004519 manufacturing process Methods 0.000 claims abstract description 37
- 235000013601 eggs Nutrition 0.000 claims abstract description 23
- 241000237515 Chlamys nipponensis Species 0.000 claims abstract description 17
- 238000009396 hybridization Methods 0.000 claims abstract description 16
- 230000004083 survival effect Effects 0.000 claims abstract description 14
- 230000001568 sexual effect Effects 0.000 claims abstract description 5
- 238000009395 breeding Methods 0.000 claims description 15
- 230000001488 breeding effect Effects 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 12
- 229920001661 Chitosan Polymers 0.000 claims description 11
- 108010000912 Egg Proteins Proteins 0.000 claims description 9
- 102000002322 Egg Proteins Human genes 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 9
- 230000003203 everyday effect Effects 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- 210000004681 ovum Anatomy 0.000 claims description 9
- 238000003306 harvesting Methods 0.000 claims description 8
- 210000004508 polar body Anatomy 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 7
- 230000017448 oviposition Effects 0.000 claims description 6
- 230000004936 stimulating effect Effects 0.000 claims description 6
- 238000011109 contamination Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000007796 conventional method Methods 0.000 claims description 4
- 210000002149 gonad Anatomy 0.000 claims description 4
- 239000013535 sea water Substances 0.000 claims description 4
- 235000015170 shellfish Nutrition 0.000 claims description 4
- 241000196317 Platymonas Species 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 2
- 238000011534 incubation Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000644 propagated effect Effects 0.000 claims description 2
- 230000005070 ripening Effects 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 241000195493 Cryptophyta Species 0.000 claims 2
- 210000004027 cell Anatomy 0.000 claims 1
- 230000004720 fertilization Effects 0.000 claims 1
- 230000032696 parturition Effects 0.000 claims 1
- 238000003860 storage Methods 0.000 claims 1
- 241000237517 Patinopecten Species 0.000 description 5
- 238000009403 interspecific hybridization Methods 0.000 description 5
- 241000206761 Bacillariophyta Species 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 3
- 235000013372 meat Nutrition 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000009404 close breeding Methods 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001969 hypertrophic effect Effects 0.000 description 1
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- 230000035764 nutrition Effects 0.000 description 1
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
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Abstract
The first-filial generation cultivation method of female patinopecten yessoensis and male alaska scallop is characterized by that firstly, respectively establishing several half-sib hybrid groups of female patinopecten yessoensis and male alaska scallop and their self-reproduction groups; selecting an optimal hybridization population, wherein corresponding parent self-reproduction populations of the optimal hybridization population become a female patinopecten yessoensis species shell production population and a male Arragaga scallop species shell production population which can be used for commercial hybridization; after the scallop reaches sexual maturity, the eggs of the female patinopecten yessoensis scallop production population and the sperms of the male Arragaga scallop production population are used for producing first-filial generation commercial offspring seeds. The obtained first-filial generation commercial offspring seed has the advantages of two types of scallops, has the advantages of fast growth, wide adaptability and strong stress resistance, is suitable for being cultured in the northern sea area of China, improves the yield by more than 20 percent compared with the Japanese scallop, improves the survival rate by about 10 percent, and is expected to be widely popularized in the future.
Description
Technical Field
The invention belongs to the shellfish breeding technology, and particularly relates to a first-filial generation cultivation method of female patinopecten yessoensis and male alaska scallops.
Background
Patinopecten yessoensis (Patinopecten yessoensis) is native to the sea areas of Japan, Russia, Korea and the like, and has been artificially cultured in large ranges in Shandong, Liaoning and the like since the Patinopecten yessoensis is introduced into China by Liaoning ocean aquatic product research institute in 1982, and has large individuals, fat scallop meat, fine meat quality, delicious taste, rich nutrition and high market value. But the growth speed is slow, the growth period is long, and the problems of frequent diseases, deterioration of seeds, high mortality and the like are obvious due to the close breeding of the generations, thereby bringing great loss to the breeding industry and seriously restricting the healthy and sustainable development of the scallop breeding industry in China. In order to solve the above problems, genetic improvement of germplasm of Japanese scallop is indispensable, and cross breeding is one of the most effective methods.
The Arragana scallop (Patinopecten saurinus) is a cold water large scallop originally produced in the northeast of the Pacific ocean, from the Arragana to the sea area of California, and has the advantages of high heterozygosity, rich genetic diversity, narrow temperature adaptation range, maximum shell length of 25cm, large shell width, hypertrophic adductor muscle, high meat yield and delicious taste, so that the processed product of the Arragana scallop is deeply welcomed in Europe and America and is one of the good culture varieties recognized in the world.
Researches show that the Patinopecten yessoensis and the Arragana scallop belong to Patinopecten, the number of chromosomes of the Patinopecten yessoensis and the number of chromosomes of the Patinopecten yessoensis are the same, and the properties of the two kinds of scallops are complementary in the aspects of individual size, growth, temperature adaptability and the like, so that interspecific hybridization is possible between the Arragana yessoensis and the Patinopecten yessoensis, and a hybrid scallop with large individual, fast growth and strong stress resistance is cultured.
Disclosure of Invention
The invention aims to provide a first-generation breeding method of female patinopecten yessoensis and male alaska scallops, which solves the problems in the existing patinopecten yessoensis breeding and breeding.
The invention is based on the following idea: 1) the Arragana scallop and the Japanese scallop belong to Patinopecten, and the number of chromosomes is the same, so that interspecific hybridization can be successfully carried out between the two scallops; 2) the characters of the two scallops are complementary in the aspects of individual size, growth, temperature adaptability and the like, so that if the two scallops are subjected to interspecific hybridization, a hybrid scallop which integrates the excellent genes of the two scallops and has the advantages of large individual, quick growth, wide temperature adaptation range and strong stress resistance can be cultured; 3) by interspecific hybridization, the germplasm resource of the Arragana scallop is utilized to improve the germplasm of the Patinopecten yessoensis, and the hybrid mating set system of the Patinopecten yessoensis and the Arragana scallop with high combining ability is cultured by respectively establishing the matching systems of the female Patinopecten yessoensis and the male Arragana scallop, so that the scallop breeding industry directly utilizes the hybrid vigor of the first filial generation of the hybrid.
The technical scheme of the invention is as follows:
1) establishment of female patinopecten yessoensis production population: respectively selecting 25 mature female and male Japanese scallops and 25 male Arlas Patinopecten, allowing each female Japanese scallop to lay eggs in a separate container by conventional method of drying in the shade and stimulating temperature, and adding 10-6Treating with chitosan for 20 min to obtain more than 10 eggs of Patinopecten yessoensis, and mixing 25 male Arragana scallop or Patinopecten yessoensis together to produce essence. Dividing each egg of egg laying patinopecten yessoensis into two parts, fertilizing with the sperm of patinopecten yessoensis and the sperm of Arragaga scallop, respectively, and establishing hybrid population of more than 10 female patinopecten yessoensis x male Arragaga scallop and corresponding selfing population of more than 10 patinopecten yessoensis. All the populations are cultured under the same condition, the optimal hybrid population is selected according to the overall expression of growth and survival traits at harvest time, and the corresponding patinopecten yessoensis inbred population becomes a female patinopecten yessoensis variety shell production population for commercial hybridization.
2) Establishment of male Arragana scallop production population: respectively selecting 25 mature male and female Arragaga scallops and 25 mature female Japanese scallops, and enabling each male Arragaga scallop to produce sperms in a separate container by using a conventional method of drying in the shade and stimulating temperature to obtain the sperms of 10 Arragaga scallops. 25 female Japanese scallops are put together to lay eggs and 10 are added-6Treating with chitosan for 20 min, mixing, dividing into 10 parts, and fertilizing with sperm of 10 Arragana scallops to establish hybrid population of 10 female Patinopecten yessoensis x male Arragana scallops. 25 female Arragaga scallops are mixed together to lay eggs, the mixture is divided into 10 parts, the 10 parts are fertilized by sperms of the 10 Arragaga scallops respectively, and 10 corresponding Arragaga scallop inbred populations are established. All the populations are cultured under the same condition, the optimal hybrid population is selected according to the overall expression of growth and survival traits at harvest, and the corresponding self-bred population of the Arragana scallops becomes a male Arragana scallop species producing population which can be used for commercial hybridization.
3) The eggs are obtained after the female patinopecten yessoensis in the female patinopecten yessoensis seed shell production population is induced to spawn, and then the sperms of the male Arragaga scallops in the male Arragaga scallop seed shell production population are fertilized, so that the first filial generation scallop seed with remarkable growth advantage can be obtained. Meanwhile, the female patinopecten yessoensis scallop seed producing population and the male Arragaga scallop seed producing population are respectively propagated to obtain a male and female patinopecten yessoensis seed producing population which can be used for producing a first filial generation in the next year.
The first filial generation seedlings obtained by the method have the excellent characteristics of the patinopecten yessoensis and the Arragaga scallop, have the advantages of quick growth, wide adaptability and strong stress resistance, are suitable for cultivation in northern sea areas of China such as the great continental sea and the Shandong long island, and have the yield improved by more than 20 percent compared with the patinopecten yessoensis and the survival rate improved by about 10 percent.
Detailed Description
Example 1
The method comprises the following specific operation steps:
1) establishment of female patinopecten yessoensis production population: respectively selecting 50 female and male Japanese scallop and 50 male Arragaga scallop, placing them into seedling-raising chamber at the beginning of 2 months, separately culturing the female and male scallop with culture density of 10 pieces/m3Feeding the diatoms such as diatoms and Platymonas with the concentration of 200-3Changing water by one half every day, pouring the pond every six days, and continuously inflating by using an air compressor. After the scallop is put into the pool, the temperature is raised to 0.5 ℃ every day, the scallop is cultivated for a plurality of days at constant temperature of 8 ℃, and the feeding amount of the bait is gradually increased until the scallop reaches sexual maturity. Respectively selecting 25 male and female patinopecten yessoensis with mature gonad and 25 male Arraxia scallop, drying in shade for 1 hr, placing single female patinopecten yessoensis in 25L plastic bucket with 12 deg.C seawater, respectively obtaining at least 10 eggs of patinopecten yessoensis, laying eggs, adding 10 eggs-6The chitosan is placed for 20 minutes and then is checked under a microscope to see whether sperm is polluted or not, if polar bodies are released, the sperm is discarded, and if the polar bodies are released, the sperm is collected; 25 male Japanese scallops or Arragaga scallops are respectively combined together to produce essence. Dividing each egg of egg laying Patinopecten yessoensis into two parts, respectively adding sperm of Arragaga scallop and sperm of Patinopecten yessoensisThe dosage is preferably 4-5 sperms around each ovum by microscopic examination, and if not enough, the addition can be continued. 10 female patinopecten yessoensis x male alaska scallop hybrid populations and corresponding patinopecten yessoensis selfing populations are respectively established. The fertilized eggs are placed in a 100L white plastic bucket for hatching, and the larva cultivation, the intermediate cultivation of the offspring seeds and the marine cultivation are carried out according to the conventional patinopecten yessoensis cultivation method. And selecting the optimal hybrid population according to the growth of the hybrid population and the overall performance of survival traits during harvesting, wherein the corresponding patinopecten yessoensis inbred population becomes a female patinopecten yessoensis variety production population for commercial hybridization.
2) Establishment of male Arragana scallop production population: respectively selecting 50 male and female scallops of Arragana and 50 female scallops of Patinopecten yessoensis, placing the male and female scallops into a seedling culture chamber at the beginning of 2 months, and separately culturing the male and female scallops with a culture density of 10 scallops/m3Feeding the diatoms such as diatoms and Platymonas with the concentration of 200-3Changing water by one half every day, pouring the pond every six days, and continuously inflating by using an air compressor. After the scallop is put into the pool, the temperature is raised to 0.5 ℃ every day, the scallop is cultivated for a plurality of days at constant temperature of 8 ℃, and the feeding amount of the bait is gradually increased until the scallop reaches sexual maturity. Respectively selecting 25 male and female Arragaga scallops with mature gonads and 25 female Patinopecten yessoensis, drying in the shade and stimulating for 1h, and placing a single male Arragaga scallop in a 25L plastic barrel which is added with seawater at 12 ℃ in advance to respectively obtain sperms of 10 Arragaga scallops. Respectively ovipositing 25 female Arragana scallop or Patinopecten yessoensis, adding 10 after ovipositing-6After the chitosan is left for 20 minutes, the sperm contamination is checked under a microscope, if the release of polar bodies indicates that the sperm contamination is present, the sperm contamination is discarded, and if the release of polar bodies is absent, the sperm contamination is collected. Dividing the ovum of each scallop into 10 parts, respectively adding 10 sperms of Arragana scallop, wherein the sperm consumption is preferably 4-5 sperms around each ovum by microscopic examination, and if not, the sperms can be continuously added. 10 female patinopecten yessoensis x male alaska scallop hybrid populations and 10 corresponding alaska scallop self-bred populations are respectively established. Hatching the fertilized eggs in a 100L white plastic barrel, and culturing the larvae and the seedlings according to the conventional patinopecten yessoensis culturing methodIntermediate cultivation and marine cultivation. And selecting an optimal hybrid population according to the overall expression of growth and survival traits at harvest, wherein the corresponding Arragana scallop self-bred population becomes a male Arragana scallop seed shell production population which can be used for commercial hybridization.
3) Cultivating first-filial generation commercial seedlings: selecting excellent female patinopecten yessoensis and male Arragaga scallop individuals from the selected female patinopecten yessoensis production population and male Arragaga scallop production population respectively, wherein the female-male ratio is 3:1, and the female patinopecten yessoensis and the male Arragaga scallop individuals are brought into a seedling raising chamber for ripening in 2 months. After the parent scallops are mature, spawning and producing sperms respectively according to a conventional spawning method, adding a small amount of sperms of the Arragana scallops after the patinopecten yessoensis spawns in a large amount, stirring to ensure that 4-5 sperms are arranged around each ovum, and culturing according to a conventional patinopecten yessoensis larva culturing and offspring intermediate culturing method after normal incubation to obtain the first-filial generation commercial offspring seeds.
4) Breeding of the hybrid shellfish production population: selecting more than 200 female and male individuals from the female patinopecten yessoensis production population according to the female-male ratio of 3:1, and obtaining the female patinopecten yessoensis breeding production population which can be used for hybridization in the next year according to the conventional patinopecten yessoensis seedling raising, seedling protecting and breeding methods. The same method is used to obtain the male Arragana scallop production population which can be used for hybridization in the next year.
Example 2
Taking 32 mature female Japanese scallops and 5 mature male Arragaga scallops at the bottom of 2016 month, respectively obtaining Japanese scallop eggs and Arragaga scallop sperms after heating and drying in the shade, and taking 10 Japanese scallop eggs-6After the chitosan is treated for 20 minutes, the sperm of the Arragana scallop is inseminated to obtain 7 female patinopecten yessoensis x male Arragana scallop hybridization child-generation families.
Measured by 2016, 12 months and 17 days, the average shell height of the optimal hybrid family is 41.0 +/-1.8 millimeters, the shell length is 39.8 +/-1.9 millimeters, the shell width is 9.1 +/-0.7 millimeters, the weight is 6.49 +/-0.99 grams, and the survival rate in the growing period is 97.1 percent; the average shell height of the control group of Japanese scallops in the same period is 38.0 +/-2.7 mm, the shell length is 36.3 +/-2.6 mm, the shell height is 7.9 +/-0.8 mm, the body weight is 4.64 +/-1.03 mm, and the survival rate in the cultivation period is 88.6%. The shell height of the hybrid family is improved by about 7.89 percent compared with the Japanese scallop, the shell length is improved by about 9.64 percent, the shell width is improved by about 15.2 percent, the body weight is improved by about 39.9 percent, and the survival rate is improved by about 9.59 percent.
Therefore, the growth rate and the survival rate of hybrid offspring cultured by interspecific hybridization of female patinopecten yessoensis and male alaska scallop are obviously improved, and the hybrid variety is proved to have obvious breeding value.
Claims (2)
1. The first-filial generation cultivation method of female patinopecten yessoensis and male alaska scallop is characterized by that in the first year 25 female patinopecten yessoensis and male alaska scallop which are mature in development are respectively selected, and the conventional method of drying in the shade and stimulating by temp. can be used to make every female patinopecten yessoensis lay eggs in separate container, and 10 portions of them are added-6Treating with chitosan for 20 min to obtain eggs of more than 10 Patinopecten yessoensis, mixing 25 male Arragana scallops together to produce essence, and mixing 25 Patinopecten yessoensis together to produce essence; dividing each egg of egg laying patinopecten yessoensis into two parts, fertilizing with the sperm of the patinopecten yessoensis and the sperm of the Arraxia scallop, respectively, and establishing more than 10 female patinopecten yessoensis x male Arraxia scallop hybrid groups and corresponding more than 10 self-bred populations of the patinopecten yessoensis; all the populations are cultured under the same condition, the optimal hybrid population is selected according to the growth and survival characters during harvesting, and the corresponding patinopecten yessoensis inbred population becomes a female patinopecten yessoensis breeding production population for commercial hybridization; respectively selecting 25 mature male and female Arragana scallops and 25 mature female Patinopecten yessoensis in the first year, and enabling each male Arragana scallop to produce sperms in a separate container by using a conventional method of drying in the shade and stimulating the temperature to obtain the sperms of 10 Arragana scallops; 25 female Japanese scallops are put together to lay eggs and 10 are added-6Treating chitosan for 20 min, mixing, dividing into 10 parts, and fertilizing with sperm of 10 Arragana scallops to establish hybrid population of 10 female Patinopecten yessoensis x male Arragana scallops; 25 female Arragana scallops are mixed together to lay eggs, the mixture is divided into 10 parts, the 10 parts are fertilized by sperms of 10 Arragana scallops respectively, and 10 phases are establishedCorresponding self-bred population of Arragaga scallops; all the populations are cultured under the same condition, the optimal hybrid population is selected according to the growth and survival characters during harvest, and the corresponding Arragana scallop self-bred population becomes a male Arragana scallop species production population for commercial hybridization; after the scallop is matured, hastening the parturition of the female patinopecten yessoensis in the female patinopecten yessoensis seed shell production population to obtain eggs, and then fertilizing by using the sperms of the male Arragana yessoensis in the male Arragana scallop seed shell production population to obtain the first filial generation scallop seed with remarkable growth advantage; meanwhile, the female patinopecten yessoensis scallop seed production population and the male Arragaga scallop seed production population are respectively propagated to obtain a female and male scallop seed production population for producing a first filial generation in the next year.
2. A first-filial generation cultivation method for female patinopecten yessoensis and male Alaska scallop is characterized in that:
1) establishment of female patinopecten yessoensis production population: respectively selecting 50 female and male Japanese scallops and 50 male Arragaga scallops, putting the male and female scallops into a seedling raising chamber at the beginning of 2 months, separately culturing the female and male scallops with the storage density of 10/m 3, feeding diatom and flat algae with the concentration of 200-300 ten thousand cells/ml for 6 times every day, and feeding 15L/m of diatom and flat algae for each time3Changing water by one half every day, pouring the pond once every six days, and continuously inflating by using an air compressor; after the scallop is put into the pool, the temperature is raised to 0.5 ℃ every day, the scallop is cultivated for several days at constant temperature of 8 ℃, and the feeding amount of the bait is gradually increased until the scallop reaches sexual maturity; respectively selecting 25 male and female patinopecten yessoensis with mature gonad and 25 male Arraxia scallop, drying in shade for 1 hr, placing single female patinopecten yessoensis in 25L plastic bucket with 12 deg.C seawater, respectively obtaining at least 10 eggs of patinopecten yessoensis, laying eggs, adding 10 eggs-6The chitosan is placed for 20 minutes and then is checked under a microscope to see whether sperm is polluted or not, if polar bodies are released, the sperm is discarded, and if the polar bodies are released, the sperm is collected; combining 25 male Japanese scallops to produce essence, and combining 25 male Arlas and scallop to produce essence; dividing ovum of each egg laying patinopecten yessoensis into two parts, and adding Alaska fanThe dosage of the shellfish sperms and the comb shell sperms is preferably 4-5 sperms around each ovum by microscopic examination, and if the dosage of the sperms is not enough, the sperms are continuously added; respectively establishing 10 female Patinopecten yessoensis X male Arraska scallop hybrid populations and corresponding Patinopecten yessoensis inbred populations; putting the fertilized eggs into a 100L white plastic bucket for hatching, and carrying out larva cultivation, intermediate seed cultivation and marine cultivation according to a conventional patinopecten yessoensis cultivation method; establishment of female patinopecten yessoensis production population: selecting an optimal hybrid population according to the growth of the hybrid population and the overall performance of survival traits during harvesting, wherein the corresponding patinopecten yessoensis inbred population becomes a female patinopecten yessoensis variety production population for commercial hybridization;
2) establishment of male Arragana scallop production population: respectively selecting 50 male and female scallops of Arragana and 50 female scallops of Patinopecten yessoensis, placing the male and female scallops into a seedling culture chamber at the beginning of 2 months, and separately culturing the male and female scallops with a culture density of 10 scallops/m3Feeding diatom and Platymonas with the concentration of 200-3Changing water by one half every day, pouring the pond once every six days, and continuously inflating by using an air compressor; after the scallop is put into the pool, the temperature is raised to 0.5 ℃ every day, the scallop is cultivated for several days at constant temperature of 8 ℃, and the feeding amount of the bait is gradually increased until the scallop reaches sexual maturity; respectively selecting 25 male and female Arragaga scallops with mature gonads and 25 female Patinopecten yessoensis, drying in the shade and stimulating for 1h, and then placing a single male Arragaga scallop into a 25L plastic barrel which is added with seawater at 12 ℃ in advance to respectively obtain sperms of 10 Arragaga scallops; mixing 25 female Arlas and scallop for oviposition, mixing 25 female Patinopecten yessoensis for oviposition, adding 10-6After the chitosan is placed for 20 minutes, the existence of sperm contamination is checked under a microscope, if polar body release indicates that fertilization is performed, the chitosan is discarded, and if the polar body release is not performed, the chitosan is collected; dividing the ovum of each scallop into 10 parts, respectively adding 10 sperms of Arragana scallops, wherein the dosage of the sperms is preferably 4-5 sperms around each ovum by microscopic examination, and if the dosage of the sperms is not enough, the sperms are continuously added; respectively establishing 10 female patinopecten yessoensis x male alaska scallop hybrid groups and 10 corresponding alaska scallop self-bred groups; placing the fertilized egg inHatching in a 100L white plastic bucket, and performing larva cultivation, intermediate seed cultivation and marine cultivation according to a conventional patinopecten yessoensis cultivation method; selecting an optimal hybrid population according to the overall expression of growth and survival traits during harvesting, wherein the corresponding Arragana scallop inbred population becomes a male Arragana scallop seed scallop production population for commercial hybridization;
3) cultivating first-filial generation commercial seedlings: selecting excellent female patinopecten yessoensis and male Arragaga scallop individuals from the selected female patinopecten yessoensis production population and male Arragaga scallop production population respectively, wherein the female-male ratio is 3:1, and the female patinopecten yessoensis and the male Arragaga scallop individuals are brought into a seedling raising chamber for ripening in 2 months; after the parent scallops are mature, spawning and producing sperms respectively according to a conventional spawning method, adding a small amount of sperms of the Arragana scallops after the patinopecten yessoensis spawns in a large amount, stirring to ensure that 4-5 sperms are arranged around each ovum, and culturing according to a conventional patinopecten yessoensis larva culturing and offspring seed intermediate culturing method after normal incubation to obtain first-filial generation commercial offspring seeds;
4) breeding of the hybrid shellfish production population: selecting more than 200 female and male individuals from the female patinopecten yessoensis production population according to the female-male ratio of 3:1, and obtaining the female patinopecten yessoensis breeding production population for hybridization in the next year according to the conventional patinopecten yessoensis seedling raising, seedling protecting and breeding method; the same procedure was used to obtain a male Arragana scallop stock production population for the next year of hybridization.
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| CN105875453A (en) * | 2016-04-28 | 2016-08-24 | 大连海洋大学 | Crossbreeding method for improving high-temperature tolerance of patinopecten yesoensis |
| CN107148928A (en) * | 2017-05-19 | 2017-09-12 | 中国海洋大学 | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids |
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