CN101103708A - Three-element crossed breeding method for Japanese scallop - Google Patents
Three-element crossed breeding method for Japanese scallop Download PDFInfo
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- CN101103708A CN101103708A CNA2007100124767A CN200710012476A CN101103708A CN 101103708 A CN101103708 A CN 101103708A CN A2007100124767 A CNA2007100124767 A CN A2007100124767A CN 200710012476 A CN200710012476 A CN 200710012476A CN 101103708 A CN101103708 A CN 101103708A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to a patinopecten yessoensis ternary cross breeding method. Firstly the artificial cultivation accelerated ripening is implemented for the Russian patinopecten yessoensis. Secondly the artificial cultivation accelerated ripening is implemented for the Chinese patinopecten yessoensis. At the same time, the gonad maturation is achieved. A heating mode is utilized to the above patinopecten yessoensis for induced spawning, and the sperms and the eggs are respectively collected. The hybridization is implemented between the Chinese female scallop eggs and the Russian male scallop sperms or between the Chinese male scallop sperms and the Russian female scallop eggs, thus the first filial generation Chinese-Russian or Russian-Chinese two sorts of patinopecten yessoensis colonies which can be cultivated within two years with the conventional suspended longline farming method. The Janpanese patinopecten yessoensis and the Chinese-Russian or the Russian-Chinese patinopecten yessoensis are taken as the parents for the artificial cultivation accelerated ripening. At the same time, the gonad maturation is achieved. A heating mode is utilized to stimulate the induced spawning, and the sperms and the eggs collected are respectively selected for the implementation of hybridization. Thereby the multiple Russian-Chinese-Japanese, Japanese-Chinese-Russian or Janpanese-Russian-Chinese patinopecten yessoensis colonies are obtained, which can be cultivated with the conventional suspended longline farming method. The method is simple, practical with low cost. The patinopecten yessoensis antiviral ability is enhanced. Moreover the survival rate and the livability are high. The offspring heterozygosity is also enhanced through the group fertilization.
Description
Technical field
The present invention relates to a kind of breeding method, particularly a kind of three-element crossed breeding method for Japanese scallop.Be to adopt the Patinopecten yessoensis of three different geographical population to carry out a kind of breeding method of three way cross specifically.
Background technology
Hybridization is meant the mating between two Different varieties or the strain parent, and this hybridization mode is called two-way cross on thremmatology.Three way cross is to utilize two kinds or population to hybridize earlier, and hybridize with the 3rd kind or population after the ontogeny sexual maturity hybrid first filial generation that is produced again, a kind of hybridization mode of the second filial that hybridizes.This hybridization mating is to carry out between three dissimilar parents, and this hybridization mode can be greater than two-way cross in heterotic utilization.At first, under whole hybridization system, the hybrid vigour of two-way cross aspect productivity can obtain utilizing, and two-way cross also is different from purebred pure lines to the action effect of ternary hybrid.Secondly, three way cross has been gathered difference of three populations and the complementary effect of three populations, and therefore the hybrid vigour on single quantitative character can be bigger.
Patinopecten yessoensis (Patinopecten yessoensis Jay) belongs to Mollusca (Mollusca), lamellibranchiata (Lamellibranchia), Pectenidae (Pectinidae) shellfish, belonging to the cold water marine shellfish, is most important economic one of kind of culturing in the Pectenidae kind.Be distributed in mainly that the Russian Far East is coastal, the birch of Thousand Islands, Japan too, the ground such as Japanese marine site of Hokkaido, this north, continent and NORTHERN KOREA.
Patinopecten yessoensis natural distribution depth of water 6-60m, substrate is a gravel, 5-20 ℃ of growth comfort zone, gonochorism has the only a few hermaphroditism, cross fertilization, breeding season female reproduction gland is a salmon pink, male is milky.Age at sexual maturity was generally for 2 ages, and China Huanghai Sea and Bohai Sea is generally by the end of March to mid-April breeding, culture mainly concentrate on Liaoning, Shandong, etc. the marine site.Propagate 17-23 month artificially, individual average shell length can reach 10cm, the heavy 100-150g of live body.Patinopecten yessoensis belongs to large-scale shellfish, the high 20cm that surpasses of maximum shell, and body weight can reach 450g, is the build maximum in the kind that China marine site distributes, and price is the highest, most popular breed kind.The meat exquisiteness, the meat flavour deliciousness is of high nutritive value, and the dried scallop that its closed shell flesh is made is one of marine products eight delicacies.Patinopecten yessoensis also is the unique cold water Pectenidae kind of carrying out large-scale cultivation in China Huanghai Sea and Bohai Sea.
At present, China produces to go up with Japan introduction colony and produces spat from numerous mode, and the shortcoming of this production method is: inbreeding causes the proterties degeneration to cause the Patinopecten yessoensis premunition poor, and survival rate is low, extensive death frequently occurs.
Summary of the invention
The purpose of this invention is to provide a kind of simple, three-element crossed breeding method for Japanese scallop that cost is low, the Patinopecten yessoensis resistance against diseases of utilizing this method to cultivate is strong, survival rate is high.
Three-element crossed breeding method for Japanese scallop of the present invention, it is characterized in that: the Patinopecten yessoensis colony parent shellfish of choosing the Patinopecten yessoensis colony in the Patinopecten yessoensis colony in Russian marine site, Chinese marine site, Japanese marine site is respectively cultivated crossbreeding as the parent, comprises the steps:
A, elder generation carry out the artificial culture accelerating to the Patinopecten yessoensis colony in Russian marine site, and a day intensification is controlled at 0.2～0.4 ℃, and wherein the effective accumulated temperature of the more conventional accelerating of effective accumulated temperature many 30～50 is subsisted, and breeding method is thrown in bait routinely;
B, again to the Patinopecten yessoensis colony in Chinese marine site routinely the accelerating mode carry out the artificial culture accelerating, make it to reach gonad maturity simultaneously with the Patinopecten yessoensis colony in Russian marine site;
C, the Patinopecten yessoensis colony that above-mentioned a, b step are cultivated utilize heating mode to stimulate and hasten parturition, collect sperm and the ovum of the smooth scallop of the two seed shrimps parent of colony shellfish respectively;
D, mating fertilization, hybridize by the female shellfish ovum in the male shellfish sperm in the male shellfish sperm in the female shellfish ovum in Chinese marine site and Russian marine site or Chinese marine site and Russian marine site and to obtain hybridizing the first filial generation smooth scallop of Sino-Russian or Russia-Sino two seed shrimps colony, and the suspended longline farming mode was formed in 2 years routinely;
E, the Patinopecten yessoensis colony that chooses Japanese marine site and above-mentioned hybridization the first filial generation Sino-Russian or Russia-Sino Patinopecten yessoensis colony that forms that obtains carry out the artificial culture accelerating as the parent, make it to reach simultaneously gonad maturity;
F, the Patinopecten yessoensis colony that reaches gonad maturity that then the e step is cultivated utilize heating mode to stimulate and hasten parturition, and collect the Sino-Russian or Russia-Sino parent of two Patinopecten yessoensis colonies shellfish ovum, sperm and the Japanese Patinopecten yessoensis parent of colony shellfish ovum, sperm respectively;
G, mating fertilization in such a way: Sino-Russian female shellfish ovum and Japanese male shellfish sperm or Russia-Sino female shellfish ovum and Japanese male shellfish sperm are hybridized, or Sino-Russian male shellfish sperm and Japanese female shellfish ovum or Russia-Sino male shellfish sperm and Japanese female shellfish ovum are hybridized, obtain Sino-Russian day or Russia-Sino day of three way cross first filial generation or day Sino-Russian or Japan-Russia in a plurality of Patinopecten yessoensis colony, conventional suspended longline farming is formed, and the gained Patinopecten yessoensis is China, Russia and Japanese three geographical population three way cross Patinopecten yessoensis according to specific mating method hybridization.
Three-element crossed breeding method for Japanese scallop of the present invention, wherein said b step to the Patinopecten yessoensis colony in Chinese marine site routinely the accelerating mode carry out the artificial culture accelerating and be later than a step Patinopecten yessoensis colony in Russian marine site was carried out the artificial culture accelerating 14～16 days; Described heating mode stimulates that to hasten parturition be that Patinopecten yessoensis was dried in the shade 1～2 hour, heats up 2～4 ℃ to stimulate again and hastens parturition.
Three-element crossed breeding method for Japanese scallop of the present invention, the water temperature in the wherein said e step during to the more conventional accelerating of Japanese Patinopecten yessoensis colony's accelerating is high 0.9～1.1 ℃, and the more conventional accelerating of effective accumulated temperature many 18～22 is subsisted; Patinopecten yessoensis the lag behind Patinopecten yessoensis in Japanese marine site in Sino-Russian or Russia-Sino hybridization first filial generation was introduced indoor beginning artificial culture accelerating in 9～15 days, initial cultivation temperature is identical with the 9th～15 day the temperature of Patinopecten yessoensis in Japanese marine site, effective accumulated temperature than conventional accelerating lack 10 subsist about.
Three-element crossed breeding method for Japanese scallop of the present invention compared with prior art, make full use of the heterosis, hybrid vigor of three geographical population of Patinopecten yessoensis, can improve the resistance of Patinopecten yessoensis to a great extent, resistance against diseases strengthens, survival rate and survival rate have been improved, because this method is the mode of the colony hybridization of Patinopecten yessoensis, promptly adopt colony that colony is fertilized, than the mating one to one in the herding, can improve colony's heterozygosity of filial generation greatly, body is laid eggs one by one, and the sperm of a plurality of individualities participates in fertilization; Body is arranged sperm one by one, the ovum of a plurality of individualities participates in fertilization, and the individual amount that participates in is bigger, these are different with fertilization pattern one to one in traditional herding, moreover the one-to-many in the herding can only be that a hero is to how female, and its offspring grows in different maternal environments, and the day after tomorrow, action effect was not quite similar separately; This method is simple, and cost is low.
Embodiment
Choose the Patinopecten yessoensis colony parent shellfish in the Patinopecten yessoensis colony in the Patinopecten yessoensis colony in Russian marine site, Chinese marine site, Japanese marine site respectively and cultivate crossbreeding, comprise the steps: as the parent
A, elder generation carry out the artificial culture accelerating to the Patinopecten yessoensis colony in Russian marine site, day heats up and is controlled at 0.2 ℃ or 0.3 ℃ or 0.4 ℃, promptly between 0.2～0.4 ℃, all can, in the artificial culture process, the effective accumulated temperature of the more conventional accelerating of effective accumulated temperature many 30 is subsisted or 35 is subsisted or 40 subsist or 45 subsist or 50 subsist, promptly 30～50 subsist between all can, breeding method is thrown in bait routinely.
B, then to the Patinopecten yessoensis colony in Chinese marine site routinely the accelerating mode carry out the artificial culture accelerating, make it to reach gonad maturity simultaneously with the Patinopecten yessoensis colony in Russian marine site; In order to guarantee that the smooth scallop of two seed shrimps can reach gonad maturity simultaneously, the Patinopecten yessoensis colony in Chinese marine site can lag behind 14 days or 15 days or 16 days acceleratings.
C, the Patinopecten yessoensis colony that above-mentioned a, b step are cultivated utilize heating mode to stimulate and hasten parturition, and collect sperm and the ovum of the smooth scallop of the two seed shrimps parent of colony shellfish respectively; Wherein heating mode stimulates that to hasten parturition be that Patinopecten yessoensis was dried in the shade 1 hour or 1.5 hours or 2 hours, promptly between 1～2 hour all can, and then heat up 2 ℃ or 3 ℃ or 4 ℃ to stimulate and hasten parturition, promptly heat up and between 2～4 ℃, all can.If lay eggs with to arrange smart effect bad, kind of shellfish can be taken out in the seawater that is positioned over normal water temperature 0.3 hour or 0.4 hour or 0.5 hour, promptly between 0.3～0.5 hour all can, be positioned over again in the seawater after the intensification, induce close shellfish to lay eggs respectively or arrange smart.
D, mating fertilization, hybridize by the female shellfish ovum in the male shellfish sperm in the male shellfish sperm in the female shellfish ovum in Chinese marine site and Russian marine site or Chinese marine site and Russian marine site and to obtain hybridizing the first filial generation smooth scallop of Sino-Russian or Russia-Sino two seed shrimps colony, and the suspended longline farming mode was formed in 2 years routinely;
E, the Patinopecten yessoensis colony that chooses Japanese marine site and above-mentioned hybridization the first filial generation Sino-Russian or Russia-Sino Patinopecten yessoensis colony that forms that obtains carry out the artificial culture accelerating as the parent, make it to reach simultaneously gonad maturity;
Water temperature during wherein to the more conventional accelerating of Japanese Patinopecten yessoensis colony's accelerating is high 0.9 ℃ or 1 ℃ or 1.1 ℃, promptly between 0.9～1.1 ℃, all can, the more conventional accelerating of effective accumulated temperature many 18 is subsisted or 19 is subsisted or 20 subsist or 21 subsist or 22 subsist, and promptly all can between subsisting 18～22.
Patinopecten yessoensis the lag behind Patinopecten yessoensis in Japanese marine site in Sino-Russian or Russia-Sino hybridization first filial generation was introduced indoor beginning artificial culture accelerating in 9～15 days, lag behind and all can in 9 days or 10 days or 15 days, initial cultivation temperature is identical with the 9th～15 day the temperature of Patinopecten yessoensis in Japanese marine site, effective accumulated temperature than conventional accelerating lack 10 subsist about.
F, the Patinopecten yessoensis colony that reaches gonad maturity that then the e step is cultivated utilize heating mode to stimulate and hasten parturition, and collect ovum, the sperm of the Sino-Russian or Russia-Sino parent of two Patinopecten yessoensis colonies shellfish ovum, sperm and the Japanese Patinopecten yessoensis parent of colony shellfish respectively.Wherein heating mode stimulates that to hasten parturition be that Patinopecten yessoensis was dried in the shade 1 hour or 1.5 hours or 2 hours, promptly between 1～2 hour all can, and then heat up 2 ℃ or 3 ℃ or 4 ℃ to stimulate and hasten parturition, promptly institute's intensification degree all can between 2～4 ℃.If lay eggs with to arrange smart effect bad, kind of shellfish can be taken out in the seawater that is positioned over normal water temperature 0.3 hour or 0.4 hour or 0.5 hour, promptly between 0.3～0.5 hour all can, be positioned over again in the seawater after the intensification, induce close shellfish to lay eggs respectively or arrange smart.
G, mating fertilization in such a way: Sino-Russian female shellfish ovum and Japanese male shellfish sperm or Russia-Sino female shellfish ovum and Japanese male shellfish sperm are hybridized, or Sino-Russian male shellfish sperm and Japanese female shellfish ovum or Russia-Sino male shellfish sperm and Japanese female shellfish ovum are hybridized, obtain Sino-Russian day or Russia-Sino day of three way cross first filial generation or day Sino-Russian or Japan-Russia in a plurality of Patinopecten yessoensis colony, conventional suspended longline farming is formed, and the gained Patinopecten yessoensis is China, Russia and Japanese three geographical population three way cross Patinopecten yessoensis according to specific mating method hybridization.
Claims (3)
1. three-element crossed breeding method for Japanese scallop, it is characterized in that: the Patinopecten yessoensis colony parent shellfish of choosing the Patinopecten yessoensis colony in the Patinopecten yessoensis colony in Russian marine site, Chinese marine site, Japanese marine site is respectively cultivated crossbreeding as the parent, comprises the steps:
A, elder generation carry out the artificial culture accelerating to the Patinopecten yessoensis colony in Russian marine site, and a day intensification is controlled at 0.2～0.4 ℃, and wherein the effective accumulated temperature of the more conventional accelerating of effective accumulated temperature many 30～50 is subsisted, and breeding method is thrown in bait routinely;
B, again to the Patinopecten yessoensis colony in Chinese marine site routinely the accelerating mode carry out the artificial culture accelerating, make it to reach gonad maturity synchronously with the Patinopecten yessoensis colony in Russian marine site;
C, the Patinopecten yessoensis colony that above-mentioned a, b step are cultivated utilize heating mode to stimulate and hasten parturition, collect sperm and the ovum of the smooth scallop of the two seed shrimps parent of colony shellfish respectively;
D, mating fertilization, hybridize by the female shellfish ovum in the male shellfish sperm in the male shellfish sperm in the female shellfish ovum in Chinese marine site and Russian marine site or Chinese marine site and Russian marine site and to obtain hybridizing the first filial generation smooth scallop of Sino-Russian or Russia-Sino two seed shrimps colony, and the suspended longline farming mode was formed in 2 years routinely;
E, the Patinopecten yessoensis colony that chooses Japanese marine site and above-mentioned hybridization the first filial generation Sino-Russian or Russia-Sino Patinopecten yessoensis colony that forms that obtains carry out the artificial culture accelerating as the parent, synchronize them to reach gonad maturity;
F, the Patinopecten yessoensis colony that reaches gonad maturity that then the e step is cultivated utilize heating mode to stimulate and hasten parturition, and collect the Sino-Russian or Russia-Sino parent of two Patinopecten yessoensis colonies shellfish ovum, sperm and the Japanese Patinopecten yessoensis parent of colony shellfish ovum, sperm respectively;
G, mating fertilization in such a way: Sino-Russian female shellfish ovum and Japanese male shellfish sperm or Russia-Sino female shellfish ovum and Japanese male shellfish sperm are hybridized, or Sino-Russian male shellfish sperm and Japanese female shellfish ovum or Russia-Sino male shellfish sperm and Japanese female shellfish ovum are hybridized, obtain Sino-Russian day or Russia-Sino day of three way cross first filial generation or day Sino-Russian or Japan-Russia in a plurality of Patinopecten yessoensis colony, conventional buoyant raft is formed, and the gained Patinopecten yessoensis is China, Russia and Japanese three geographical population three way cross Patinopecten yessoensis according to specific mating method hybridization.
2. three-element crossed breeding method for Japanese scallop according to claim 1 is characterized in that: described b step to the Patinopecten yessoensis colony in Chinese marine site routinely the accelerating mode carry out the artificial culture accelerating and be later than a step Patinopecten yessoensis colony in Russian marine site was carried out the artificial culture accelerating 14～16 days; Described heating mode stimulates that to hasten parturition be that Patinopecten yessoensis was dried in the shade 1～2 hour, heats up 2～4 ℃ to stimulate again and hastens parturition.
3. three-element crossed breeding method for Japanese scallop according to claim 1 and 2 is characterized in that: the water temperature in the described e step during to the more conventional accelerating of Japanese Patinopecten yessoensis colony's accelerating is high 0.9～1.1 ℃, and the more conventional accelerating of effective accumulated temperature many 18～22 is subsisted; Patinopecten yessoensis the lag behind Patinopecten yessoensis in Japanese marine site in Sino-Russian or Russia-Sino hybridization first filial generation was introduced indoor beginning artificial culture accelerating in 9～15 days, initial cultivation temperature is identical with the 9th～15 day the temperature of Patinopecten yessoensis in Japanese marine site, effective accumulated temperature than conventional accelerating lack 10 subsist about.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361467B (en) * | 2008-09-25 | 2011-07-20 | 大连水产学院 | Common intertidal shellfish hybridization method |
| CN101361469B (en) * | 2008-09-25 | 2012-05-23 | 大连水产学院 | Common intertidal shellfish polymerization hybridization breeding method |
| CN104206327A (en) * | 2014-08-27 | 2014-12-17 | 青岛海弘达生物科技有限公司 | Purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application |
| WO2016029492A1 (en) * | 2014-08-27 | 2016-03-03 | 王春德 | Method for establishing three-line assorted breeding system for argopecten purpuratus-argopecten irradians irradians hybrid scallops and application thereof |
| CN107148928A (en) * | 2017-05-19 | 2017-09-12 | 中国海洋大学 | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids |
| CN109287533A (en) * | 2018-09-17 | 2019-02-01 | 大连海洋大学 | Scallop hybrid seed method for scaled breeding and equipment |
| CN110036953A (en) * | 2019-04-28 | 2019-07-23 | 江苏农林职业技术学院 | A kind of breeding method of snakehead |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
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- 2007-08-16 CN CNA2007100124767A patent/CN101103708A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101361469B (en) * | 2008-09-25 | 2012-05-23 | 大连水产学院 | Common intertidal shellfish polymerization hybridization breeding method |
| CN101361467B (en) * | 2008-09-25 | 2011-07-20 | 大连水产学院 | Common intertidal shellfish hybridization method |
| CN104206327A (en) * | 2014-08-27 | 2014-12-17 | 青岛海弘达生物科技有限公司 | Purple scallop ovum and bay scallop sperm hybrid scallop maintainer line construction method and application |
| WO2016029492A1 (en) * | 2014-08-27 | 2016-03-03 | 王春德 | Method for establishing three-line assorted breeding system for argopecten purpuratus-argopecten irradians irradians hybrid scallops and application thereof |
| US10973209B2 (en) | 2014-08-27 | 2021-04-13 | Qingdao Agricultural University | Peruvian scallop X bay scallop hybrid three-line breeding system and method |
| CN107148928A (en) * | 2017-05-19 | 2017-09-12 | 中国海洋大学 | A kind of breeding method of Patinopecten yessoensis and wind vane scallop hybrids |
| CN107148928B (en) * | 2017-05-19 | 2020-07-24 | 中国海洋大学 | Method for cultivating hybrid variety of patinopecten yessoensis and wind vane scallop |
| CN109287533B (en) * | 2018-09-17 | 2024-02-27 | 大连海洋大学 | Large-scale breeding method and equipment for hybrid scallop offspring seeds |
| CN109287533A (en) * | 2018-09-17 | 2019-02-01 | 大连海洋大学 | Scallop hybrid seed method for scaled breeding and equipment |
| CN110036953A (en) * | 2019-04-28 | 2019-07-23 | 江苏农林职业技术学院 | A kind of breeding method of snakehead |
| CN110036953B (en) * | 2019-04-28 | 2021-04-27 | 江苏农林职业技术学院 | Snakehead fish cultivation method |
| CN116391654A (en) * | 2023-03-30 | 2023-07-07 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
| CN116391654B (en) * | 2023-03-30 | 2023-11-03 | 中国海洋大学 | Large-scale hybridization breeding method for hermaphrodite scallops |
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