CN101438689A - Seed production method of two shell color lines of Mactra chinensis - Google Patents

Seed production method of two shell color lines of Mactra chinensis Download PDF

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Publication number
CN101438689A
CN101438689A CNA2008102299525A CN200810229952A CN101438689A CN 101438689 A CN101438689 A CN 101438689A CN A2008102299525 A CNA2008102299525 A CN A2008102299525A CN 200810229952 A CN200810229952 A CN 200810229952A CN 101438689 A CN101438689 A CN 101438689A
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shell
look
clam
larva
parent
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Inventor
闫喜武
张跃环
王�琦
霍忠明
赵越
杨凤
张国范
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Dalian Fisheries University
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Dalian Fisheries University
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a breeding method of two shell color strains of mactra chinensis. A qualitative character that is the shell color is taken as a mark, and the mactra chinensis with two shell colors are taken as materials to obtain the two shell color strains of the mactra chinensis by directed breeding generation by generation. Particularly, the two shell color strains comprise a yellow brown mactra strain and a green brown mactra strain. The method comprises the following steps: self-breeding the groups of the same shell color strain and establishing a self-bred family to quicken the purification speed of the shell color by combining the directed breeding, and selecting two shell color strains with accordant shell colors and significant difference. The obtained two shell color stains lay a foundation for the shell color strain development and quality improvement of the mactra chinensis. The method has the advantages of convenient operation and easy popularization.

Description

The producing method for seed of two shell chromaticity systems of China clam
Technical field
The present invention relates to marine biotechnology, is shellfish genetic breeding and germplasm improvement field, the producing method for seed of two shell chromaticity systems of a kind of specifically Chinese clam.
Background technology
The China clam is subordinate to lamellibranchiata, curtain clam order. and clam section, clam belongs to, and mainly is distributed in the ground (Zhang Xi, 1961) such as Liaoning, Shandong and Japan, Korea of China.The China clam perches the place, shallow sea, nearly river mouth unimpeded at current, that bait is abundant, and the place is maximum with the 2-5m depth of water, until the sea area of the depth of water 60m distribution is arranged all, the meat flavour deliciousness, and the dressing percentage height is a kind of intertidal shellfish that better nutritivity is worth that has.Yan Xiwu etc. (2008) have broken through the artificial breeding of Chinese clam and have formed technology, are the exploitation of intertidal shellfish indigenous species, realize the shallow sea resource recovery and safeguard that the bio-diversity of intertidal shellfish lays a good foundation.
The color of ocean Bivalve shell only is used as secretory product and out in the cold always in the past.In fact the color of Bivalve shell is not only relevant with behavior with their ecology also relevant with its growth, survival isophenous proterties.In China, Zhang Guofan etc. (2000-2005) are mark with the shell look, " gulf, the middle section Red sea scallop " that selects.Zhao Hongen etc. (1999) find in exploring and study about the agate Bao, grow soon the characteristics that premunition is strong because the red shell Bao that gene mutation produces has.Yan Xiwu etc. (2002-2008) find in to Ruditapes philippinarum research, the Ruditapes philippinarum of different shell looks grows and exists difference and this qualitative character of shell look to give the offspring by genetic stability, has successively obtained a series of Ruditapes philippinarum shell look breeding line and hybridization system.He Maoxian etc. (2006) cultivate by monomer and to have obtained a red shell chromaticity system in the research to pteria martensii.Chang Yaqing etc. (2007) select the ivory white shell chromaticity of Patinopecten yessoensis system according to the shell look of Patinopecten yessoensis.And there is fairly simple shell color polymorphism in Chinese CONCHA MERETRICID SEU CYCLINAE look, probably by the existing form (Yan Xiwu, 2008) of 3-5 kind shell color table.
Summary of the invention
At above phenomenon, it is seed selection work that scholars have carried out the shell chromaticity, through the directive breeding in 2 years, has obtained two China CONCHA MERETRICID SEU CYCLINAE chromaticity system, and this haves laid a good foundation for the exploitation of Chinese clam new varieties.
The invention provides a kind of is the producing method for seed of the Chinese CONCHA MERETRICID SEU CYCLINAE chromaticity system of mark with the shell look, utilizes this qualitative character of shell look to be mark, is material with the Chinese clam of different shell looks, obtains to have the shell chromaticity system of merit by the directive breeding by generation.
The one-tenth shellfish background color of yellowish-brown China clam strain is a yellowish-brown, but has the radiation band, and young shellfish is radiated the band distinctness, has informal voucher band and narrower informal voucher band of two broads, also has two one dark one shallow red stripes; The background color that green and brown look Chinese clam product are tied to form shellfish and young shellfish is green and brown look, has the radiation band, but not obvious.
The present invention is that Chinese clam with yellowish-brown, green and brown look is as reproductive population, after parent's maturation with the parent of identical housings look as male parent, female parent, impel close shellfish to lay eggs, arrange smart by the method for artificial stimulation, through laying eggs and hatching, larva are cultivated, juvenile mollusk is cultivated, breed link in the middle of the juvenile mollusk, obtained the Chinese clam strain of the different shell looks of first filial generation; Treat the filial generation gonad maturity, in the time of can laying eggs, arrange essence, put in a suitable place to breed as close shellfish with the first filial generation of identical housings look, and carry out directive breeding in an identical manner, thereby obtain second filial, so circulation goes down to make that the shell look is further purified; Obtain Chinese CONCHA MERETRICID SEU CYCLINAE look new lines.
The concrete steps of its producing method for seed are as follows:
1), close shellfish chooses and accelerating: from wild population or cultured population, the shell mould of selecting 2-3 age is regular, and the yellowish-brown of shell look unanimity, green and brown look China clam are put in the ecological shrimp pond and carry out ecological accelerating as reproductive population, and gonad development is observed in sampling regularly;
2), lay eggs and hatch: close shellfish gonad maturity, different shell looks China clam parents are placed separately, through drying in the shade 3-6h, 2-4h is approximately passed through in the stimulation of flowing water 2-3h, the parent row that begins to lay eggs is smart; Adopt colony's selfing of identical housings look China clam and set up self-fertilization family (sff), fertilized egg hatching density is controlled at 30-40/ml; Under temperature 24-25 ℃ condition, fertilized egg is approximately all hatched to D shape larva through 23h;
3), larva cultivates: with the net cage seed selection D shape larva that 320 mesh sieve tulles are made, larva culture density 5-6 is individual/ml, changes water every day 1 time, and quantity of exchanged water is 30-60%; Bait is thrown something and fed 2 times every day, the pavlova viridis of throwing something and feeding early stage, and the later stage is mixed Zhanjiang Isochrysis galbana, chlorella, the tower born of the same parents algae of the volume ratio 1:1:1 that throws something and feeds, and the suitable larva of daily ration, feeding quantity ingests situation and decides, and changes network of rivers packing list and solely uses; During the larvae cultivation, water temperature is 24.4-27.6 ℃, and salinity is 24-28;
4), juvenile mollusk is cultivated: behind the larval metamorphosis, the normal cultivation) with 3, the suitable juvenile mollusk of daily ration, feeding quantity is ingested situation and is decided, approximately through the cultivation of 40-50d, the juvenile mollusk shell reaches 2-3mm, this moment juvenile mollusk is transferred to ecological shrimp pond and carries out young shellfish and breed, during this period, temperature is 23-30 ℃, and salinity is 24-28, and pH is 7.52-8.86;
5), the centre is bred: the centre is bred and is adopted 60 order mesh bags earlier, according to the juvenile mollusk size, regularly is replaced with 40 orders, 20 purpose mesh bags successively;
6), pursuing of shell chromaticity system: links such as the process larva is cultivated, juvenile mollusk is cultivated, the centre is bred for purifying, obtained the Chinese clam strain of two kinds of shell looks of first filial generation, cultivation through the anniversary, the filial generation gonad maturity, can lay eggs, thereby the acquisition second filial, so circulation goes down to make that the shell look is further purified, and therefrom filters out the shell chromaticity system fast, strong stress resistance of growing.
Take strict quarantine measures in the operating process of laying eggs according to a conventional method, hatching, promptly smart the beginning laid eggs, arranged to the parent cleans to hatching into all utensils and the facility that D shape larval stage adopted, and it is smart more same shell look China clam parent to be put into the same container row of laying eggs after the parent cleaned separately, collect the smart ovum of monomer, set up the self-fertilization family (sff) of identical housings look, its production of hybrid seeds effect is better.
The parent chooses in the process, if with two kinds of shell aberration different obviously and the now very consistent two kinds of shell colo(u)r group bodies of shell color table as the parent, then more help the shell colour purity process of filial generation.
Lay eggs in the hatching process, be optimal way as follows: utilize identical housings look parent to carry out spawning and fertilization, i.e. identical housings look parent's colony's selfing and monomer selfing avoids variegated parent's individuality to sneak into, and filial generation shell look is impacted.In the whole process, the strict isolation, guarantee the existing uniformity of filial generation shell color table.
Seed rearing and forming in the process, the filial generation of different shell looks strict to be isolated, and avoids confusion, and guarantees the uniformity of filial generation shell look.Then production of hybrid seeds effect is better.
Shell chromaticity system pursues in the purge process, and optimal way is: select the now highly consistent individuality of shell color table from the first filial generation, as the parent, breed second filial; Adopt same way as to iterate selection later on, can further accelerate shell colour purity speed like this.
Specific embodiment
Further specify the present invention with Chinese clam below, but be not construed as limiting the invention.
Embodiment 1
The first step, close shellfish are chosen and accelerating: at the beginning of 2007 5 months, at Dalian Zhuanghe point mountain beach, the shell mould of selecting for 3 ages is regular, the yellowish-brown of shell look height unanimity, green and brown look China clam are as reproductive population 10kg, adopt the scallop cage to raise in cages, be put in the shrimp pond of Dalian magnanimity aquatic food Co., Ltd and carry out ecological accelerating.Gonad development is observed in sampling regularly, treats its gonad maturity.
Second step, lay eggs and hatch: in early July, 2007, close shellfish gonad maturity is placed different shell looks China clam parents separately, and through drying in the shade 3-6h, the Da Bai bucket of 100L is put in the stimulation of flowing water 2-3h, approximately passes through 2-4h, parent's row's essence that begins to lay eggs.In order to improve incubation rate, fertilized egg hatching density is controlled at 30-40/ml.Under temperature 24-25 ℃ condition, fertilized egg is approximately all hatched to D shape larva through 23h.During this period, two kinds of shell look parents place separately, avoid mixing, and the smart ovum of two shell chromaticity systems is also wanted strict the isolation.
The 3rd step, larva are cultivated: with the net cage seed selection D shape larva that 320 mesh sieve tulles are made, cultivate in the white plastic bucket of placement 100L, and larva culture density 5-6/ml, change water every day 1 time, quantity of exchanged water is 30-60%.Bait is thrown something and fed 2 times every day, the pavlova viridis of throwing something and feeding early stage, and the later stage is mixed Zhanjiang Isochrysis galbana, chlorella, the tower born of the same parents algae of the volume ratio 1:1:1 that throws something and feeds.The suitable larva of daily ration, feeding quantity ingests situation and decides.For preventing to mix between the different shell looks Chinese clam larva, change network of rivers packing list and solely use.During the larvae cultivation, water temperature is 25-27 ℃, and salinity is 24-28.
The 4th step, juvenile mollusk are cultivated: behind the larval metamorphosis, and normal the cultivation with the 3rd step.Increase because juvenile mollusk is individual, food ration increases, and the suitable juvenile mollusk of daily ration, feeding quantity is ingested situation and decided.Approximately through the cultivation of 40-50d, the juvenile mollusk shell reaches 2-3mm, this moment juvenile mollusk is transferred to ecological shrimp pond and carries out young shellfish and breed.During this period, being that Chinese clam juvenile mollusk is strict to two shell chromaticities isolates, and avoids confusion, and the water quality situation is temperature 26-30 ℃, salinity 24-27, pH7.52-8.86.
Breed in the middle of the 5th step, the young shellfish: employing 60 order mesh bags are bred in the centre, and (40cm * 60cm), breed the stage in the middle of juvenile mollusk, the mesh bag (60 orders-40 order-20 order) of periodic replacement different meshes are eliminated the influence of mesh to young shellfish growth.Make the young shellfish of each shell chromaticity system hang foster consistent in density, eliminate the influence of density growth.In the process of changing bag, avoid obscuring between shell chromaticity system, guarantee the existing uniformity of shell color table.
The 6th step, shell chromaticity system by for purifying: through larva cultivate, link such as juvenile mollusk is cultivated, the centre is bred, obtained the Chinese clam of two kinds of shell looks of first filial generation.At the beginning of 2008 7 months, the first filial generation gonad maturity as the parent, iterates selection to the shell look with the first filial generation, through above step, has obtained the second filial of two shell chromaticity systems of Chinese clam, and the shell colour purity rate of second filial is more than 98%.
Through above step, by the directive breeding in two generations, obtained two shell chromaticity systems of Chinese clam, its shell colour purity rate is more than 98%, for the exploitation of shell look new varieties is laid a good foundation.
Embodiment 2
The first step, close shellfish are chosen and accelerating: at the beginning of 2007 5 months, at Dalian Zhuanghe point mountain beach, the shell mould of selecting for 3 ages is regular, the yellowish-brown of shell look height unanimity, green and brown look China clam are as reproductive population 10kg, adopt the scallop cage to raise in cages, be put in the shrimp pond of Dalian magnanimity aquatic food Co., Ltd and carry out ecological accelerating.Gonad development is observed in sampling regularly, treats its gonad maturity.
Second step, lay eggs and hatch: in early July, 2007, close shellfish gonad maturity is placed different shell looks China clam parents separately, through drying in the shade 3-6h, and the stimulation of flowing water 2-3h, 2-4h is approximately passed through in the big red listing of putting into 20L, parent's row's essence that begins to lay eggs.At this moment, the smart individuality of row of laying eggs is crawled out, adopt identical housings look parent to carry out list, successfully set up self-fertilization family (sff) and 9 self-fertilization family (sff)s that green and brown look shell chromaticity is of 6 yellowish-brown strains single crosses.During this period, two kinds of shell look parents place separately, avoid mixing, and the smart ovum of two shell chromaticity systems is also wanted strict the isolation.
The 3rd step, larva are cultivated: with the net cage seed selection D shape larva that 320 mesh sieve tulles are made, cultivate in the white plastic bucket of placement 100L, and larva culture density 5-6/ml, changed water 1 time in per 2 days, quantity of exchanged water is 100%.Bait is thrown something and fed 2 times every day, the pavlova viridis of throwing something and feeding early stage, and the later stage is mixed Zhanjiang Isochrysis galbana, chlorella, the tower born of the same parents algae of the volume ratio 1:1:1 that throws something and feeds.The suitable larva of daily ration, feeding quantity ingests situation and decides.For preventing to mix between the different shell looks Chinese clam self-fertilization family (sff) larva, change network of rivers packing list and solely use.During the larvae cultivation, water temperature is 25-27 ℃, and salinity is 24-28.
The 4th step, juvenile mollusk are cultivated: behind the larval metamorphosis, and normal the cultivation with the 3rd step.Increase because juvenile mollusk is individual, food ration increases, and the suitable juvenile mollusk of daily ration, feeding quantity is ingested situation and decided.Approximately through the cultivation of 40-50d, the juvenile mollusk shell reaches 2-3mm, this moment juvenile mollusk is transferred to ecological shrimp pond and carries out young shellfish and breed.During this period, being that Chinese clam juvenile mollusk is strict to two shell chromaticities isolates, and avoids confusion, and the water quality situation is temperature 26-30 ℃, salinity 24-27, pH7.52-8.86.
Breed in the middle of the 5th step, the young shellfish: employing 60 order mesh bags are bred in the centre, and (40cm * 60cm), breed the stage in the middle of juvenile mollusk, the mesh bag (60 orders-40 order-20 order) of periodic replacement different meshes are eliminated the influence of mesh to young shellfish growth.Make the young shellfish of each shell chromaticity system hang foster consistent in density, eliminate the influence of density growth.In the process of changing bag, avoid obscuring between shell chromaticity system, guarantee the existing uniformity of shell color table.
What the 6th step, shell chromaticity were pursues for purifying: links such as the process larva is cultivated, juvenile mollusk is cultivated, the centre is bred have obtained 3 yellowish-brown strains and 5 self-fertilization family (sff) first filial generations that green and brown look shell chromaticity is.At the beginning of 2008 7 months, the first filial generation gonad maturity, with the first filial generation as the parent, shell look to the parent iterates selection, adopts selfing strategy in the family, through above step, the second filial that to have obtained two shell chromaticities of Chinese clam be self-fertilization family (sff), the shell colour purity rate of second filial is close to 100%.
Through above step, iterate selection in the family by two generations, obtained two shell chromaticity systems of Chinese clam, its shell colour purity rate is close to 100%, for the exploitation of shell look new varieties is laid a good foundation.
Embodiment 3
The first step, close shellfish are chosen and accelerating: at the beginning of 2008 4 months, at Dalian Zhuanghe point mountain beach, the shell mould of selecting for 3 ages is regular, the yellowish-brown of shell look unanimity, green and brown look China clam are as reproductive population 10kg, adopt the scallop cage to raise in cages, be put in the shrimp pond of Dalian magnanimity aquatic food Co., Ltd and carry out ecological accelerating.Gonad development is observed in sampling regularly, treats its gonad maturity.
Second step, lay eggs and hatch: in early July, 2007, close shellfish gonad maturity is placed different shell looks China clam parents separately, and through drying in the shade 3-6h, the red basin of 20L is put in the stimulation of flowing water 2-3h, approximately passes through 2-3h, parent's row's essence that begins to lay eggs.At this moment, the smart individuality of row of laying eggs is crawled out, adopt uneven Nested Design, set up self-fertilization family (sff) and 18 self-fertilization family (sff)s that green and brown look shell chromaticity is of 6 yellowish-brown strains.In order to improve incubation rate, fertilized egg hatching density is controlled at 20-30/ml.Under temperature 23-24 ℃ condition, fertilized egg is approximately all hatched to D shape larva through 24h.During this period, the smart ovum of the self-fertilization family (sff) of two shell chromaticity systems is wanted strict isolation, in order to avoid obscure.
The 3rd step, larva are cultivated: with the net cage seed selection D shape larva that 320 mesh sieve tulles are made, cultivate in the white plastic bucket of placement 100L, and larva culture density 4-5/ml, changed water 1 time in per 2 days, quantity of exchanged water is 100%.Bait is thrown something and fed 2 times every day, the pavlova viridis of throwing something and feeding early stage, and the later stage is mixed Zhanjiang Isochrysis galbana, chlorella, the tower born of the same parents algae of the volume ratio 1:1:1 that throws something and feeds.The suitable larva of daily ration, feeding quantity ingests situation and decides.For preventing to mix between the different shell looks Chinese clam self-fertilization family (sff) larva, change network of rivers packing list and solely use.During the larvae cultivation, water temperature is 24-26 ℃, and salinity is 24-28, pH7.82-8.30.
The 4th step, juvenile mollusk are cultivated: behind the larval metamorphosis, and normal the cultivation with the 3rd step.Increase because juvenile mollusk is individual, food ration increases, and the suitable juvenile mollusk of daily ration, feeding quantity is ingested situation and decided.Approximately through the cultivation of 40-50d, the juvenile mollusk shell reaches 2-3mm, this moment juvenile mollusk is transferred to ecological shrimp pond and carries out young shellfish and breed.During this period, be strict isolation of Chinese clam juvenile mollusk of self-fertilization family (sff) to two shell chromaticities, to avoid confusion, the water quality situation is temperature 26-30 ℃, salinity 24-27, pH7.52-8.86.
Breed in the middle of the 5th step, the young shellfish: employing 60 order mesh bags are bred in the centre, and (40cm * 60cm), breed the stage in the middle of juvenile mollusk, the mesh bag (60 orders-40 order-20 order) of periodic replacement different meshes are eliminated the influence of mesh to young shellfish growth.Make the self-fertilization family (sff) children shellfish of each shell chromaticity system hang foster consistent in density, eliminate the influence of density growth.In the process of changing bag, avoid obscuring between shell chromaticity system, guarantee the existing uniformity of shell color table.
What the 6th step, shell chromaticity were pursues for purifying: links such as the process larva is cultivated, juvenile mollusk is cultivated, the centre is bred, obtain the first filial generation that two kinds of shell chromaticities of Chinese clam are, and the shell colour purity rate of first filial generation is more than 99%.
Through above step, obtained the self-fertilization family (sff) first filial generation of identical housings chromaticity system, the shell colour purity rate of its filial generation is closely more than 99%, and as seen, the self-fertilization family (sff) of identical housings look has quickened the process of shell colour purityization, helps the exploitation of shell look new varieties.

Claims (5)

1, the producing method for seed of two shell chromaticity systems of a kind of Chinese clam, it is characterized in that may further comprise the steps: 1) close shellfish is chosen and accelerating; 2) lay eggs and hatch; 3) larva is cultivated; 4) juvenile mollusk is cultivated; 5) breed in the middle of the young shellfish; 6) shell chromaticity system pursues for purifying;
1), close shellfish chooses and accelerating: from wild population or cultured population, the shell mould of selecting 2-3 age is regular, and the yellowish-brown of shell look unanimity, green and brown look China clam are put in the ecological shrimp pond and carry out ecological accelerating as reproductive population, and gonad development is observed in sampling regularly;
2), lay eggs and hatch: close shellfish gonad maturity, different shell looks China clam parents are placed separately, through drying in the shade 3-6h, 2-4h is approximately passed through in the stimulation of flowing water 2-3h, the parent row that begins to lay eggs is smart; Adopt colony's selfing of identical housings look China clam and set up self-fertilization family (sff), fertilized egg hatching density is controlled at 30-40/ml; Under temperature 24-25 ℃ condition, fertilized egg is approximately all hatched to D shape larva through 23h;
3), larva cultivates: with the net cage seed selection D shape larva that 320 mesh sieve tulles are made, larva culture density 4-6 is individual/ml, changes water every day 1 time, and quantity of exchanged water is 30-60%; Bait is thrown something and fed 2 times every day, the pavlova viridis of throwing something and feeding early stage, and the later stage is mixed Zhanjiang Isochrysis galbana, chlorella, the tower born of the same parents algae of the volume ratio 1:1:1 that throws something and feeds, and the suitable larva of daily ration, feeding quantity ingests situation and decides, and changes network of rivers packing list and solely uses; During the larvae cultivation, water temperature is 24.4-27.6 ℃, and salinity is 24-28;
4), juvenile mollusk is cultivated: behind the larval metamorphosis, the normal cultivation) with 3, the suitable juvenile mollusk of daily ration, feeding quantity is ingested situation and is decided, approximately through the cultivation of 40-50d, the juvenile mollusk shell reaches 2-3mm, this moment juvenile mollusk is transferred to ecological shrimp pond and carries out young shellfish and breed, during this period, temperature is 23-30 ℃, and salinity is 24-28, and pH is 7.52-8.86;
5), the centre is bred: the centre is bred and is adopted 60 order mesh bags earlier, according to the juvenile mollusk size, regularly is replaced with 40 orders, 20 purpose mesh bags successively;
6), pursuing of shell chromaticity system: links such as the process larva is cultivated, juvenile mollusk is cultivated, the centre is bred for purifying, obtained the Chinese clam strain of two kinds of shell looks of first filial generation, cultivation through the anniversary, the filial generation gonad maturity, can lay eggs, thereby the acquisition second filial, so circulation goes down to make that the shell look is further purified, and therefrom filters out the shell chromaticity system fast, strong stress resistance of growing.
2, the producing method for seed of two shell chromaticity systems of Chinese clam as claimed in claim 1, it is characterized in that: the parent chooses in the process, with two kinds of shell aberration different obviously and the now very consistent two kinds of shell colo(u)r group bodies of shell color table as the parent, help the shell colour purity process of filial generation like this.
3, the producing method for seed of two shell chromaticity systems of Chinese clam as claimed in claim 1, it is characterized in that: in the hatching process of laying eggs, utilize identical housings look parent to carry out spawning and fertilization, be identical housings look parent's colony's selfing and monomer selfing, avoid variegated parent's individuality to sneak into, filial generation shell look is impacted.In the whole process, the strict isolation, guarantee the existing uniformity of filial generation shell color table.
4, the producing method for seed of two shell chromaticities of Chinese clam as claimed in claim 1 system is characterized in that: seed rearing and forming in the process, the filial generation of different shell looks strict to be isolated, and avoids confusion, and guarantees the uniformity of filial generation shell look.
5, the producing method for seed of two shell chromaticity systems of Chinese clam as claimed in claim 1 is characterized in that: the now highly consistent individuality of shell color table is selected by in the purge process by shell chromaticity system from the first filial generation, as the parent, breed second filial; Adopt same way as to iterate selection later on, can further accelerate shell colour purity speed like this.
CNA2008102299525A 2008-12-17 2008-12-17 Seed production method of two shell color lines of Mactra chinensis Pending CN101438689A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101861839A (en) * 2010-04-29 2010-10-20 上海海洋大学 Method for artificially breeding purple nacre strain hyriopsis cumingii
CN103125415A (en) * 2013-03-19 2013-06-05 广西壮族自治区海洋研究所 Artificial breeding method of tapes dorsatus
CN104082527A (en) * 2014-07-17 2014-10-08 魏巍 Pearl oyster breeding bait material
CN108849657A (en) * 2018-07-17 2018-11-23 北海市秀派珠宝有限责任公司 A kind of pteria martensii incubation breeding method
CN109548723A (en) * 2018-12-20 2019-04-02 龙口市温流水育苗养殖有限公司 A kind of scallop culture method

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101861839A (en) * 2010-04-29 2010-10-20 上海海洋大学 Method for artificially breeding purple nacre strain hyriopsis cumingii
CN103125415A (en) * 2013-03-19 2013-06-05 广西壮族自治区海洋研究所 Artificial breeding method of tapes dorsatus
CN103125415B (en) * 2013-03-19 2014-08-06 广西壮族自治区海洋研究所 Artificial breeding method of tapes dorsatus
CN104082527A (en) * 2014-07-17 2014-10-08 魏巍 Pearl oyster breeding bait material
CN104082527B (en) * 2014-07-17 2016-05-04 安徽金钗石斛有限公司 A kind of pearl culturing clam bait
CN108849657A (en) * 2018-07-17 2018-11-23 北海市秀派珠宝有限责任公司 A kind of pteria martensii incubation breeding method
CN109548723A (en) * 2018-12-20 2019-04-02 龙口市温流水育苗养殖有限公司 A kind of scallop culture method

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