CN101120665A - Seedling culture method for fresh water pearl culturing clam - Google Patents

Seedling culture method for fresh water pearl culturing clam Download PDF

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Publication number
CN101120665A
CN101120665A CNA2007101318172A CN200710131817A CN101120665A CN 101120665 A CN101120665 A CN 101120665A CN A2007101318172 A CNA2007101318172 A CN A2007101318172A CN 200710131817 A CN200710131817 A CN 200710131817A CN 101120665 A CN101120665 A CN 101120665A
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China
Prior art keywords
parasitic
water
tilapia
temporarily
larva
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CNA2007101318172A
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Chinese (zh)
Inventor
华丹
顾若波
闻海波
徐钢春
王玉芳
杨健
刘洪波
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Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences
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Freshwater Fisheries Research Center of Chinese Academy of Fishery Sciences
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Priority to CNA2007101318172A priority Critical patent/CN101120665A/en
Publication of CN101120665A publication Critical patent/CN101120665A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The present invention relates to a spat-cultivating method of the freshwater pearl mussel, which belongs to the technical field of the aquatic spat cultivation. The present invention is characterized in selecting the tilapia; the tilapia is temporarily cultivated after immersed in the sodium chloride solution; taking off the outer gill lamella on both sides of the mature mother mussel to put into a container having the water, moving aside the branchial filament with a pin and filtering the gills and the mucus in the water with a bolting silk to separate the lumpish glochidium; putting the temporarily-cultivated tilapia into a parasitic water body and then put into a temporarily-cultivated cylinder to cultivate, the glochidia completes the transformation and the larva mussel completes the shedding. The present invention selects the tilapia having a strong adversity resistance as the parasitic fish for the freshwater cultivated pearly mussel to realize the parasitic transformation and development process of the gloachidia; the parasitic quantity is large; the survival rate of the tilapia is very high in the parasitic period; in addition, the price of the tilapia is low, which can reduce the spat cultivation cost; the present invention has a favorable economic benefit in the freshwater mussel large-scale spat breeding process.

Description

A kind of seedling-cultivating method of fresh water pearl culturing clam
Technical field
The present invention relates to a kind of seedling-cultivating method of fresh water pearl culturing clam, specifically utilize the parasitic fish of Tilapia mossambica, realize the parasitic development by metamorphosis of hook Jie larva, belong to the aquatic fry growing technical field as fresh water pearl culturing clam.
Background technology
At present, the fresh water pearl culturing clam of China mainly comprises kinds such as hydriopsis cumingii, cristaria plicata, circle anodonta woodiana pacifica, anodonta woodiana pacifica, all is under the jurisdiction of and belongs to Mollusca Mollusca, Bivalvia Bivalvia, Eulamellibranchia Eulamellibranchia, Unionidae Unionidae species.The Unionidae kind is the hydrobiological important group of China's fresh water, and its unique history of life is other marine bivalves of difference obviously.Their fertilized egg develops into hook Jie larva on the parent gill filament, discharge parent after hook Jie larva maturation,, be attached on its gill filament, the fin ray when running into suitable parasitic fish, parasitic metamorphosis could further develop into juvenile mollusk through of short duration fish body, drops to the life of dwelling at the end of water-bed battalion from the fish body.
In fresh water pearl culturing clam seedling growing process field, be to realize the parasitic development by metamorphosis of hook Jie larva, need manually select for use parasitic fish that hook Jie larva of maturation is carried out parasitism, by to the supporting temporarily of parasitic fish short section time, obtain the juvenile mollusk seed.
In prior art, growing seedlings of China's fresh water pearl culturing clam adopted following two kinds usually: 1, the hydriopsis cumingii seedling raising process adopts yellow cartfish to make parasitic fish more, lethality height when parasitic, and yellow cartfish price height generally at 40 yuan/kilogram, makes seedling cost strengthen.2, cristaria plicata is grown seedlings and generally use the bighead fingerling, and the parasitic quantity of every tail fish is 100-200 larva, parasitism stage fingerling lethality height, and parasitic efficient is lower.Therefore, improve the parasitic efficient of hook Jie larva, reduce seedling cost, searching out parasitic fish more suitably, to be used for the device for sedling growth of fresh water pearl culturing clam significant.
Summary of the invention
The objective of the invention is to overcome above-mentioned weak point; thereby provide a kind of seedling-cultivating method of fresh water pearl culturing clam; can overcome the high mortality of conventional parasitic fish in the fresh water pearl culturing clam reproductive process; the Tilapia mossambica of selecting strong stress resistance for use is as parasitic fish; improve the parasitic efficient of hook Jie larva; reduce the shellfish reproductive-cost, in the production of fresh water pearl culturing clam scale breeding, great using value is arranged.
Main solution of the present invention is achieved in that
The seedling-cultivating method of fresh water pearl culturing clam of the present invention adopts following processing step:
1, supported 7~15 days temporarily after the Tilapia mossambicas that select 50~200 grams are soaked 10~20min with the sodium chloride solution of 5%-10%;
2, get the freshwater mussel that breeds of 2~3 fresh water pearl culturing clams, check whether larva is ripe; Take off the outer gill lamella on ripe female freshwater mussel both sides, be placed in the container that water is housed, push the gill filament aside also with 20~60 mesh sieve thin,tough silk the elimination gill and mucus in water with pin, hook Jie larva of bulk is separated, with clear water the larva that disperses is diluted, make larval density be: 10~15/ml, the parasitic water body of parasitic fish is at 5~6L;
(3), then in parasitic water body, put into and support the back Tilapia mossambica temporarily, air-pump inflating, aeration quantity is at 8~12L/min, and the parasitic time is: 10~25min;
(4), will pull out and support temporarily the back Tilapia mossambica, put into the temporary vatting of parasitic fish and support temporarily again and cultivate, water temperature remains between 18~28 ℃;
(5), cultivated 5~19 days, hook Jie larva is finished metamorphosis, juvenile mollusk comes off and finishes.
Described parasitic water body volume increases by 8~12 tails/5~6L in proportion with parasitic fish quantity.
Compared with the prior art the present invention has the following advantages:
The present invention selects the parasitic fish of the Tilapia mossambica of strong stress resistance as fresh water pearl culturing clam for use, realizes the parasitic development by metamorphosis process of hook Jie larva; Parasitic quantity is big; Parasitic fish survival rate height between parasitic stage; The parasitic fish price is low, and seedling cost is reduced; In freshwater shellfish scale breeding process, use, will have good economic benefit.
Embodiment
Following the present invention will be further described in conjunction with the embodiments:
Embodiment one:
The present invention adopts following processing step to the hydriopsis cumingii seedling-cultivating method:
Select healthy Tilapia mossambica, the yellow cartfish that hinders that do not have, the sodium chloride solution with 5% soaks to support temporarily after 10 minutes and a week is used for parasitism.2, get 2 hydriopsis cumingiis and breed freshwater mussel, check whether larva is ripe.Anatomical lens is checked down and is found: the larva rupture of membranes, and with 10~15 times/minute open and close movement, show that larva is ripe and parasitic ability is arranged.3, the female freshwater mussel two outer gill filaments are taken out, break the gill filament, provoke with dissecting needle larva is dispersed in the water body,, be beneficial to the parasitism of larva with 40 mesh sieve thin,tough silk the elimination gill and mucus in water with scissors.4, with clear water the larva that disperses is diluted, make larval density keep 15/ml, the parasitic water body of parasitic fish remains on 6L.5, with Tilapia mossambica, each 10 tail of yellow cartfish, put into above-mentioned parasitic water body simultaneously, air-pump inflating (aeration quantity is at 8L/min), parasitic about 10 minutes, respectively fish is pulled out, support temporarily in the aquarium of same specification respectively.Hang the similar polyethylene net cage of specification (40 order) in aquarium, net bottom and cylinder bottom prevent that apart from 10cm parasitic fish from causing infringement to abnormal juvenile mollusk.6, under 24~25 ℃ of conditions of water temperature, since the 5th day, inhale parasitic fish cylinder bottom portion with siphonage, shine thin,tough silk with 200 orders and filter, with the juvenile mollusk counting statistics of metamorphosis.5~13 days larval metamorphosis in parasitic back substantially all come off.
The result shows: Tilapia mossambica is organized the 3291/tail of juvenile mollusk quantity that on average comes off, parasitic fish survival rate 100%; Yellow cartfish is organized the 2250/tail of juvenile mollusk that on average comes off, parasitic fish survival rate 70%.
Embodiment two:
The present invention adopts following processing step to circle anodonta woodiana pacifica seedling-cultivating method:
1, select healthy Tilapia mossambica, yellow cartfish, the bighead that hinders that do not have, the sodium chloride solution with 10% soaks to support temporarily after 15 minutes and was used for parasitism in 10 days.2, get 3 round anodonta woodiana pacificas and breed freshwater mussel, check whether larva is ripe.Anatomical lens is checked down and found: larva shows that larva is ripe and parasitic ability is arranged with 10~15 times/minute open and close movement.3, the female freshwater mussel two outer gill filaments are taken out, break the gill filament, provoke with dissecting needle larva is dispersed in the water body,, be beneficial to the parasitism of larva with 40 mesh sieve thin,tough silk the elimination gill and mucus in water with scissors.4, with clear water the larva that disperses is diluted, make larval density keep 10/ml, the parasitic water body of parasitic fish remains on 5L.5, with Tilapia mossambica, yellow cartfish, each 10 tail of bighead, put into above-mentioned parasitic water body simultaneously, air-pump inflating (aeration quantity is at 10L/min), parasitic about 15 minutes, respectively fish is pulled out, support temporarily in the aquarium of same specification respectively.Hang the similar polyethylene net cage of specification (40 order) in aquarium, net bottom and cylinder bottom prevent that apart from 12cm parasitic fish from causing infringement to abnormal juvenile mollusk.6, under 18~19 ℃ of conditions of water temperature, since the 11st day, inhale parasitic fish cylinder bottom portion with siphonage, filter with 200 mesh sieve thin,tough silk, with the juvenile mollusk counting statistics of metamorphosis.11~19 days larval metamorphosis in parasitic back substantially all come off.
The result shows: Tilapia mossambica is organized the 2045/tail of juvenile mollusk quantity that on average comes off, parasitic fish survival rate 100%; Yellow cartfish is organized the 287/tail of juvenile mollusk that on average comes off, parasitic fish survival rate 60%; Bighead is organized the 220/tail of juvenile mollusk quantity that on average comes off, parasitic fish survival rate 30%.
Embodiment three:
The present invention adopts following processing step to oval anodonta woodiana pacifica seedling-cultivating method:
Select healthy Tilapia mossambica, the yellow cartfish that hinders that do not have, the sodium chloride solution with 10% soaks to support temporarily after 15 minutes and was used for parasitism in 10 days.2, get 2 oval anodonta woodiana pacificas and breed freshwater mussel, check whether larva is ripe.Anatomical lens is checked down and found: larva shows that larva is ripe and parasitic ability is arranged with 10~15 times/minute open and close movement.3, the female freshwater mussel two outer gill filaments are taken out, break the gill filament, provoke with dissecting needle larva is dispersed in the water body,, be beneficial to the parasitism of larva with 40 mesh sieve thin,tough silk the elimination gill and mucus in water with scissors.4, will disperse larva to be diluted to certain density with clear water, and make larval density keep 10/ml, the parasitic water body of parasitic fish remains on 6L.5, with Tilapia mossambica, each 10 tail of yellow cartfish of choosing, put into above-mentioned parasitic water body simultaneously, air-pump inflating (aeration quantity is at 12L/min), parasitic about 15 minutes, respectively fish is pulled out, support temporarily in the aquarium of same specification respectively.Hang the similar polyethylene net cage of specification (40 order) in aquarium, net bottom and cylinder bottom prevent that apart from 15cm parasitic fish from causing infringement to abnormal juvenile mollusk.6, under 24~25 ℃ of conditions of water temperature, since the 5th day, inhale parasitic fish cylinder bottom portion with siphonage, filter with 200 mesh sieve thin,tough silk, with the juvenile mollusk counting statistics of metamorphosis.5~9 days larval metamorphosis in parasitic back substantially all come off.
The result shows: Tilapia mossambica is organized the 245/tail of juvenile mollusk quantity that on average comes off, parasitic fish survival rate 90%; Yellow cartfish is organized the 134/tail of juvenile mollusk that on average comes off, parasitic fish survival rate 70%.

Claims (2)

1. the seedling-cultivating method of a fresh water pearl culturing clam is characterized in that adopting following processing step:
(1), the Tilapia mossambica that selects 50~200 grams was supported 7~15 days after 10~20 minutes temporarily with 5%~10% sodium chloride solution immersion;
(2), get the freshwater mussel that breeds of 2-3 fresh water pearl culturing clam; Take off the outer gill lamella on ripe female freshwater mussel both sides, be placed in the container that water is housed, push the gill filament aside also with 20~60 mesh sieve thin,tough silk the elimination gill and mucus in water with pin, hook Jie larva of bulk is separated, with clear water the larva that disperses is diluted, make larval density be: 10~15/ml, the parasitic water body of parasitic fish is at 5~6L;
(3), then in parasitic water body, put into and support the back Tilapia mossambica temporarily, air-pump inflating, aeration quantity is at 8~12L/min, and the parasitic time is: 10~25min;
(4), will pull out and support temporarily the back Tilapia mossambica, put into the temporary vatting of parasitic fish and support temporarily again and cultivate, water temperature remains between 18~28 ℃;
(5), cultivated 5~19 days, hook Jie larva is finished metamorphosis, juvenile mollusk comes off and finishes.
2. the seedling-cultivating method of a kind of fresh water pearl culturing clam according to claim 1 is characterized in that described parasitic water body volume increases by 8~12 tails/5~6L in proportion with parasitic fish quantity.
CNA2007101318172A 2007-09-05 2007-09-05 Seedling culture method for fresh water pearl culturing clam Pending CN101120665A (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101647416B (en) * 2009-09-08 2011-07-20 中国水产科学研究院淡水渔业研究中心 Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool
CN106489803A (en) * 2016-10-20 2017-03-15 中国水产科学研究院淡水渔业研究中心 The artificial screening system of fresh water bivalves hook Jie's larva host symptoms
CN112262797A (en) * 2020-10-28 2021-01-26 安徽水韵环保股份有限公司 Artificial breeding method of giant clam
CN112471017A (en) * 2020-12-17 2021-03-12 安徽水韵环保股份有限公司 Artificial breeding technology of fish tail wedge mussel
CN112616735A (en) * 2020-12-31 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding technology of cave limussels
CN112616734A (en) * 2020-12-30 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding method of calotte pearl mussel
CN112616733A (en) * 2020-12-18 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding method of mussels
CN112690234A (en) * 2021-01-18 2021-04-23 安徽水韵环保股份有限公司 Artificial breeding method of young solenopsis amabilis based on host fish
CN115380853A (en) * 2022-09-21 2022-11-25 南昌大学 Method for propagating endangered freshwater shellfish

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101647416B (en) * 2009-09-08 2011-07-20 中国水产科学研究院淡水渔业研究中心 Method for net fishing, transporting and temporarily culturing coilia ectenes parent fish in pool
CN106489803A (en) * 2016-10-20 2017-03-15 中国水产科学研究院淡水渔业研究中心 The artificial screening system of fresh water bivalves hook Jie's larva host symptoms
CN106489803B (en) * 2016-10-20 2019-08-02 中国水产科学研究院淡水渔业研究中心 The artificial screening system of fresh water bivalves hook Jie's larva host symptoms
CN112262797A (en) * 2020-10-28 2021-01-26 安徽水韵环保股份有限公司 Artificial breeding method of giant clam
CN112471017A (en) * 2020-12-17 2021-03-12 安徽水韵环保股份有限公司 Artificial breeding technology of fish tail wedge mussel
CN112616733A (en) * 2020-12-18 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding method of mussels
CN112616734A (en) * 2020-12-30 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding method of calotte pearl mussel
CN112616735A (en) * 2020-12-31 2021-04-09 安徽水韵环保股份有限公司 Artificial breeding technology of cave limussels
CN112690234A (en) * 2021-01-18 2021-04-23 安徽水韵环保股份有限公司 Artificial breeding method of young solenopsis amabilis based on host fish
CN115380853A (en) * 2022-09-21 2022-11-25 南昌大学 Method for propagating endangered freshwater shellfish

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