CN103719006A

CN103719006A - Method for improving survival rate of coilia ectenes parents during artificial breeding period

Info

Publication number: CN103719006A
Application number: CN201410026730.9A
Authority: CN
Inventors: 施永海; 张海明; 张根玉; 谢永德; 陆根海; 刘建忠; 徐嘉波; 刘永士
Original assignee: SHANGHAI AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION; Shanghai Fisheries Research Institute
Current assignee: Shanghai Fisheries Research Institute Shanghai Fisheries Technical Extension Station
Priority date: 2014-01-21
Filing date: 2014-01-21
Publication date: 2014-04-16
Anticipated expiration: 2034-01-21
Also published as: CN103719006B

Abstract

The invention provides a method for improving the survival rate of coilia ectenes parents during an artificial breeding period. The method is characterized by comprising the following steps of adopting artificially-bred offspring coilia ectenes adult fish which is more than two years old as reserve parents of artificial breeding; in the cultivating process of the reserve parents, pulling a net and turning over a tank for 2-3 times; before the artificial breeding, pulling the net of the reserve parents and training for 2-3 times; selecting the coilia ectenes adult fish with a completely-developed gonad as parents; conveying the selected parents by using a light-shielding dark bucket; slightly operating with water and naturally fertilizing, wherein the dosage of an oxytocic hormone is not more than 30 micrograms per kilogram and the normal saline amount per fish is not more than 0.5ml, a hasten parturition tank is circular or square; shielding and regulating; continuously micro-inflating; after the parents lay eggs, carrying out postpartum intensified cultivation; gradually adding seawater to adjust the salinity to 5-10, wherein the released density is 20-40 per tank; continuously inflating and controlling the water temperature to 22-25 DEG C; feeding opossum shrimps and palaemon carinicauda for two times per day; determining the feeding amount of the next time according to the food ration; and sucking the bottom for one time per day to remove dead fish and shrimps in time.

Description

A kind of method that improves cutter long-tailed anchovy parent survival rate during artificial breeding

Technical field

The present invention relates to aquaculture, especially improve the method for parent survival rate during cutter long-tailed anchovy artificial breeding.

Background technology

Cutter long-tailed anchovy ( coilia nasusschlegel), having another name called long jaw long-tailed anchovy, be commonly called as hairtail, rough cutter fish, is sea, river migration fishes, is mainly distributed in the yellow Bohai Sea of China and the East Sea one band, and all maritime rivers all have distribution, the highest with THE LOWER YANGTZE VALLEY output.Coilia ectenes in Yangtze River is strong and famous with fine and tender taste, fresh fertilizer, seasonal, and with the Changjiang river hilsa herring, filefish and claim " the Changjiang river three delicacies ", in history, Coilia ectenes in Yangtze River resource is extremely abundant.In recent years, due to the impact of the factors such as overfishing and the deterioration of the ecological environment, cutter long-tailed anchovy resource sharply fails, and output is downward trend year by year and individual miniaturization is serious, cutter long-tailed anchovy can not form dominant population in the Changjiang river, and the price of Coilia ectenes in Yangtze River marketable fish is also in continuous rising simultaneously.Cutter long-tailed anchovy artificial breeding is supported breakthrough and the popularization of technology, and then advances the industrialization breeding production of this fish, can promote the adjustment of fishery constitution, impels fishery synergy, fisherman's increased income.Therefore, cutter long-tailed anchovy has good development prospect as propagating new varieties artificially.At present, about the propagation technique research of cutter long-tailed anchovy is scarcely out of swaddling-clothes, in recent years, the research of this correlation technique is carried out in the numerous and confused project verification of domestic many scientific research institutions, occasionally there are indivedual reports of cutter long-tailed anchovy pond reproductive success, although the success that base, Shanghai Aquatic Product Institute Fengxian adopted the cutter long-tailed anchovy parent of culture to take the lead in obtaining indoor intensive artificial breeding in 2011, but because cutter long-tailed anchovy is had a fiery temperament, there is no the reasons such as good cultivation in artificial propagation misoperation and postpartum, after artificial breeding, cutter long-tailed anchovy parent survival rate is extremely low, also caused the feritilization of ovum rate of artificial propagation gained low simultaneously, the ropy problem of newly hatched larvae, therefore improving cutter long-tailed anchovy parent survival rate during artificial breeding is the key technology of cutter long-tailed anchovy artificial propagation.

Summary of the invention

The object of this invention is to provide a kind of method that improves cutter long-tailed anchovy parent survival rate during artificial breeding, to adapt to, the scale of cutter long-tailed anchovy is intensive breeds production.

Technical scheme of the present invention is selected, hastened parturition by parent's preparation, parent, and fertilization is controlled, reinforced cultivating production stage forms, it is characterized in that adopting artificial breeding to support forming 2 age above filial generation cutter long-tailed anchovy adult fish as the standby parent of artificial propagation, with respect to wild or wild seed, propagate the adult fish forming artificially, these filial generation cutter long-tailed anchovy adult fish disposition passives, in standby parent breeding process, draw in the net to turn over pond 2-3 time, simultaneously before artificial breeding, standby parent draws in the net to take exercise 2-3 time again, the stress reaction causing because of manual operation to reduce parent; Select gonad development complete, select the cutter long-tailed anchovy adult fish of 4 phases of gonad development as parent, otherwise grow not adult fish meeting mortality in the process of hastening parturition completely, select parent's standard: raun postabdomen expands and soft, and cloacal aperture is micro-red; Milter, gently presses cloacal aperture to have a small amount of seminal fluid to flow out; The parent of select adopts the dark drum transportation of shading; Whole select with transportation in operation softly not dried up; Oxytocic hormone dosage is no more than 30ug/Kg, and the every tail of saline volume is no more than 0.5ml, can't adopt heavy dose of hormone and large capacity physiological saline, causes parent's death; Adopt natural insemination mode, otherwise adopt test-tube words, checking whether raun lays eggs and manually squeeze ovum and cause in squeezing smart process the injured and scale of parent to come off; Spawning inducing pool adopts round or square pond, shading regulation and control, and continuous micro-inflation, daily management condition is with the conventional mating system of cutter long-tailed anchovy; After parent is laid eggs, carry out reinforced cultivating in postpartum, cultivate the same spawning inducing pool of pond condition postpartum, progressively add seawater and adjust salt to salinity 5-10, to play antiinflammation, breeding density is 20-40 tail/pond simultaneously, continuous charge, water temperature keeps 22-25 ℃, throw something and feed for 2 times every day oppossum shrimp and Exopalaemon carinicauda, depending on food ration, fix inferior feeding volume, inhale the end 1 time every day, remove in time dead fishes and shrimps, the cultivation time in postpartum is 7-10 days, through whole reproductive process, parent survival rate is at 85-95%.

The present invention with respect to the outstanding feature of prior art is: greatly improved the survival rate of cutter long-tailed anchovy during breeding; survival rate is at 85-95%; with respect to original 20-40%, be significantly improved; simultaneously; also indirectly improved the quality of fertilized egg and prelarva; in addition, the method is easy and simple to handle, method is practical, be applicable to cutter long-tailed anchovy propagation in scale produces, and is easy to be accepted and promote.

Embodiment

1, parent prepares: adopt artificial breeding to support to form 2 age above filial generation cutter long-tailed anchovy adult fish as the standby parent of artificial propagation, in standby parent breeding process, draw in the net to turn over pond 2-3 time, the while, standby parent drew in the net to take exercise 2-3 time again before artificial breeding.

2, parent selects: select gonad development complete, select the cutter long-tailed anchovy adult fish of 4 phases of gonad development as parent, select parent's standard: raun postabdomen expands and soft, and cloacal aperture is micro-red; Milter, gently presses cloacal aperture to have a small amount of seminal fluid to flow out; The parent of select adopts the dark drum transportation of shading; Whole select with transportation in operation softly not dried up.

3, the control of hastening parturition and be fertilized: oxytocic hormone dosage is no more than 30ug/Kg, and the every tail of saline volume is no more than 0.5ml; Adopt natural insemination mode, otherwise adopt test-tube words, checking whether raun lays eggs and manually squeeze ovum and cause in squeezing smart process the injured and scale of parent to come off; Spawning inducing pool adopts round or square pond, and spawning inducing pool area round or square is 20 square metres, shading regulation and control, and continuous micro-inflation, daily management condition is with the conventional mating system of cutter long-tailed anchovy;

4, postpartum reinforced cultivating: after parent is laid eggs, carry out reinforced cultivating in postpartum, cultivate the same spawning inducing pool of pond condition postpartum, progressively add seawater and adjust salt to salinity 5-10, breeding density is 20-40 tail/pond, continuous charge simultaneously, water temperature keeps 22-25 ℃, throw something and feed for 2 times every day oppossum shrimp and Exopalaemon carinicauda, the feeding volume of fixing time depending on food ration, inhales the end 1 time every day, remove in time dead fishes and shrimps, the cultivation time in postpartum is 7-10 days, and through whole reproductive process, parent survival rate is at 85-95%.

Above-mentioned embodiment divides 6 batches of warps the Technique of Raising Seeedling centre base test cutter long-tailed anchovy parent of this institute 5-July in 2013, carry out whole artificial propagation, 6 batches of parent's 279 tails of hastening parturition altogether, through whole breeding and cultivating process in postpartum, parent's 248 tails of finally surviving altogether, average survival is 88.9%.

Claims

1. one kind is improved the method for cutter long-tailed anchovy parent survival rate during artificial breeding, by parent's preparation, parent, being selected, hastened parturition, fertilization is controlled, reinforced cultivating forms, it is characterized in that adopting artificial breeding to support forming 2 age above filial generation cutter long-tailed anchovy adult fish as the standby parent of artificial propagation, in standby parent breeding process, draw in the net to turn over pond 2-3 time, before artificial breeding, standby parent draws in the net to take exercise 2-3 time again; Select gonad development complete, the cutter long-tailed anchovy adult fish of 4 phases of gonad development, as parent, selects parent's standard: raun postabdomen expands and soft, and cloacal aperture is micro-red; Milter, gently presses cloacal aperture to have a small amount of seminal fluid to flow out; The parent of select adopts the dark drum transportation of shading; Whole select with transportation in operation softly not dried up; Oxytocic hormone dosage is no more than 30ug/Kg, and the every tail of saline volume is no more than 0.5ml, adopts natural insemination mode; Spawning inducing pool adopts the round or square pond of 20 square metres, shading regulation and control, and continuous micro-inflation, daily management is with the conventional mating system of cutter long-tailed anchovy; After parent is laid eggs, carry out reinforced cultivating in postpartum, cultivate the same spawning inducing pool of pond condition postpartum, progressively add seawater simultaneously and adjust salt to salinity 5-10, breeding density is 20-40 tail/pond, continuous charge, water temperature keeps 22-25 ℃, throw something and feed for 2 times every day oppossum shrimp and Exopalaemon carinicauda, depending on food ration, fix inferior feeding volume, inhale the end 1 time every day, remove in time dead fishes and shrimps, the cultivation time in postpartum is 7-10 days.

2. a kind of method that improves cutter long-tailed anchovy parent survival rate during artificial breeding according to claim 1, the spawning inducing pool area that it is characterized in that round or square is 20 square metres.