CN105379650A - Method for obtaining batch American shad fertilized ova - Google Patents
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Abstract
A method for obtaining batch American shad fertilized ova is characterized in that a plastic greenhouse cement pond is used for reserve parent overwintering culture, local fresh water is used, the salinity is increased gradually during water changing till the final salinity reaches 12%o to 15%o, the water temperature for overwintering culture is kept at more than 8 DEG C, and bait is NO.3 feeds of commercial freshwater fish expanded pellet feeds; parent spring intensive culture is between the end of March and the beginning of April, and as the temperature rises, two sides of the plastic greenhouse are used for ventilation, plastic film is dismounted, shading film is added, and the water temperature in a cement pond is kept between 16 DEG C and 18 DEG C; the salinity is reduced gradually each time water is changed, and the salinity is reduced by 2%o to 3%o each time till the final salinity reaches the salinity of local river water; parents for promoting spawning are selected to be put in curved spawning promoting channels between the middle of May and the beginning of June, the stocking density of the spawning promoting parents is between 0.8 shad/m<2> and 1 shad/m<2>, a natural spawning fertilization mode is used, the fertilized ova are collected in time after the parents spawn, the operation is performed in water, and the fertilized ova are moved to an incubating pond after counting.
Description
Technical field
The present invention relates to aquaculture, especially a kind of method obtaining batch America hilsa herring fertilized egg.
Background technology
America hilsa herring (Alosasapidissima) is commonly called as Alosa sapidissima, is mainly distributed in the river, Florida State of west bank, the Atlantic Ocean, North America from Quebec, Canada to the U.S. and ocean.America hilsa herring system breeding migration fish, plump big and fleshy, meat delicate flavour is beautiful, and the economic worth that tool is very high, is extensively introduced a fine variety all over the world.At home, Shanghai Aquatic Product Institute took the lead in 1998 introducing a fine variety from the U.S.'s Mississippi fertilized egg that wild America hilsa herring produces, and then carried out incubating oosperm, seed rearing and cultivation.Due to America hilsa herring because, meat similar to the profile of Chinese hilsa herring compare favourably, when the Changjiang river hilsa herring is endangered, America hilsa herring is also very popular, the market price is also very high, 200-300 unit/jin, raiser cultivates this fish one after another, and the seed of this fish has formed wide market prospects.Due to the reproductive habit of this fish uniqueness, at present, America hilsa herring breeds cultivation and adopts per family and extract underground less than 150 meters, winter-spring season water temperature stability carries out parent's cultivation of this fish at the phreatic water of 16-17 DEG C, then direct mode of collecting fertilized egg in parent's culturing pool, but Deep Underground water resource is limited, also region limitation is had, and a lot of coastal cities have limited Deep Underground water for aquaculture, and existing mode technical parameter relatively blindness of collecting fertilized egg in hilsa herring adult fish culture pond, America, contingency larger, these limit the supply of seed batch and the large-scale production of America hilsa herring.Therefore, be badly in need of a set of technology, maintaining under water temperature condition without phreatic water, adopt the river of natural water area or estuarine water to complete America hilsa herring parent culture, promote production natural mating and fertilization collect, to obtain the fertilized egg of batch, adapt to the batch supply of American shad fry and fingerlings.
Summary of the invention
The present invention needs the problem solved to be to provide a kind of method obtaining batch America hilsa herring fertilized egg.
Technical scheme of the present invention by standby parent overwintering culture, parent's reinforced cultivating in spring, the parent that promotes production selects, parent promotes production, natural spawning fertilization and fertilized egg are collected production stage and formed, it is characterized in that artificial breeding to support 2 age above America hilsa herring adult fish as the standby parent of artificial propagation, enter standby parent overwintering culture selecting healthy and strong, build grossness, body weight 0.75-1.5Kg commodity adult fish the annual 11-12 month, adopt plastic tunnel cement pit cultivation standby parent, employing pool wall is smooth, area is 150-250m
2square or circular cement pit, dark 1.2-1.8m, bow-shaped ring top is set above cement pit, top covers transparent heat insulating plastic film, shade membrane cover on a plastic film, light regulating by the rate of sheltering from heat or light 90%, reduces light transmittance, regulate the hiding rare of the film that shades according to pond water water colour, hiding rare maintains 30%-40% simultaneously, place gas head in cement pit, gas head density is 0.2-0.3/m
2, with Roots blower continuous charge in 24 hours, tolerance is with boiling-like, to cement pit sterilization before putting in a suitable place to breed, water inlet 20cm, full pool spilling head after dissolving with 20PPm bleaching powder, soak after 48 hours, draining is rinsed well, dries and re-uses for 3-4 days, when the standby parent selected puts in a suitable place to breed, adopt local fresh water, put latter one week in a suitable place to breed, when changing water at every turn, progressively improve salinity, rise salinity 2-3 ‰ at every turn, 12-15 ‰ is reached to last salinity, liter salt and overwintering culture water all adopt local salinity to be 10-15 ‰ estuarine water, useful water all adopts and precipitates through pond one-level, plastic tunnel cement pit nature sunshine pre-heating temperature elevation and two-stage precipitation, filter with 80 mesh sieve tulles again, change weekly water 1 time, each 2/3, every 25-35 days, turn over pond 1 time, overwintering culture water temperature remains on more than 8 DEG C, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C, bait is No. 3, commercial fresh-water fishes expanded pellet feed material, and crude protein content is 38%, throws something and feeds every day 1 time, and having ingested with 1-2 hour is as the criterion, cultivate excreta at the bottom of pond and the corrupt and polluted water of residual bait deposition to alleviate, put Exopalaemon carinicauda in a suitable place to breed in cultivation pond, stocking rate is 5-10 jin/pond, to by the end of March at the beginning of-4 months, enter parent's reinforced cultivating in spring stage, now in plastic tunnel cement pit, water temperature has just started to reach 15-16 DEG C, along with the rising of outside temperature, adopt plastic tunnel both sides to ventilate, remove plastic film, add a cover ground floor shade film, add a cover the second layer again and to shade film, keep water temperature 16-18 DEG C in cement pit, when changing water at every turn, progressively reduce salinity, each salt reduction degree 2-3 ‰, reaches local river to last salinity, salt reduction and reinforced cultivating water all adopt local river, useful water all adopts through pond one-level precipitation, shade booth cement pit Temperature fall and two-stage precipitation, then filters with 80 mesh sieve tulles, and every 5-6 days changes water 1 time, each 2/3, turned over pond every 2 weeks 1 time, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C, bait is No. 3, commercial fish expanded pellet feed material, and crude protein content is more than 40%, throws something and feeds every day 2 times, and having ingested with 1-2 hour is as the criterion, other management are the same, at the beginning of-6 months mid-Mays, the booth that now shades cultivates pool inner water temperature 17-19 DEG C, and parent raun belly expands successively, now can select the use parent that promotes production, criteria for selection: raun postabdomen expands and soft, and cloacal aperture is micro-red, and some gently presses belly to have ovum to flow out, milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out, and chance water does not fall apart, the parent of select adopts 100L drum to transport and puts into circuit of promoting production, put 2-3 tail parent for every barrel, whole selecting wants soft not dried up with operation in transportation, circuit of promoting production adopts 30.0m × 5.0m × 1.2m rectangle cement pit, depth of water 1.0m, through constructing intermediate isolating wall and smearing corner, round pond, form 60.0m × 2.4m × closed circuit of 1.1m ellipse, 0.75KW waterwheel aerator is adopted to carry out oxygenation and form current, 10KW air source heat pump water heater group is adopted to keep water temperature stability in circuit by cold and hot radiating tube, above cement pit is, bow-shaped ring top is set, top covers the film that shades of three layers of rate 90% of sheltering from heat or light, ensure that the intensity of illumination on daytime is lower than 300Lux, parent's breeding density of promoting production is 0.8 ~ 1 tail/m
2, sex ration is 1:1.1 ~ 1:1.3, and water temperature of promoting production is 17.5 ~ 19 DEG C, after parent enters circuit of promoting production, keep absolutely quiet around spawning inducing pool, adopt natural spawning fertilization mode, remaining closed circuit water velocity is 0.07 ~ 0.11m/s, and water temperature of promoting production is 17.5 ~ 19 DEG C, to promote production a small amount of expanding compound feed of throwing something and feeding in pond, absorb fertilized egg to reduce parent, but residual bait will be removed in time, otherwise expanded pellet diet is immersed in the time in water has grown and will sink to the bottom, be mixed into fertilized egg, cause fertilized egg to clean and removing impurities difficult, America hilsa herring parent has the custom of fertilized egg of ingesting, parent will collect fertilized egg after laying eggs in time, before collecting fertilized egg, suspend waterwheel aerator, after allowing flow rate of water flow lower, copy at the bottom of pond, place, circuit dead angle with 24 order soft bolting silk triangle diddle-net is flat, America hilsa herring egg membrane is very thin, and fertilized egg can not dried uply extrude, collecting ovum will be with water to operate, after copying ovum, then open aerator 3-5 minute at every turn, allow ovum automatic turning to circuit dead angle place, and then copy ovum, after repeating 3 times, then the ovum collecting of more than 95% can be cleared up, count after move on to hatchery.
The present invention relative to the outstanding feature of prior art is: 1) during standby parent overwintering culture, utilizes the water temperature of sunshine and plastic film intensification cultivation water in natural situation, when heating without the energy, keeps temperature of cultivation more than 8 DEG C; During parent's reinforced cultivating in spring, when natural temperature is higher than 18 DEG C, adopt ventilation and the film that shades to reduce the water temperature of cultivation water, keep cultivating water temperature lower than 18 DEG C; Promoting production period, adopting air energy unit to reduce and the water temperature of maintenance water; Therefore, at whole parent culture with between breeding period, do not use phreatic water (winter-spring season 16-17 DEG C) heating and cooling to maintain the water temperature of parent culture water, do not make breeding water with phreatic water yet, this protects and valuable descends phreatic water resource cleanly, also saves the energy; 2) during standby parent overwintering culture, adopt the river mouth brackish water of local natural water area, the brackish water resource that this Appropriate application is abundant, which save clean freshwater resources; 3) when drawing in the net for a long time to move pond exercise during parent culture, adopt and select and move the method that the good parent of gonad development focuses on circuit natural spawning mating fertilization of promoting production, this, than existing method of directly collecting fertilized egg in parent's culturing pool, has many good qualities, as; Promoting production in circuit, it is higher to grow good female male parent rate of meeting, and the probability of natural mating is also relatively high, also prevent sexual gland and does not develop into dried fish and disturb parent's natural mating and absorb the fertilized egg of producing; It also avoid simultaneously due to conventional parent's culturing pool internal contamination thing and residual bait relatively many, cause collect fertilized egg cleaning be separated difficulty; 4) adopt this technical scheme can obtain large batch of America hilsa herring fertilized egg, operate more easy, method is more practical, also indirectly improves the quality of fertilized egg and prelarva, is applicable to America hilsa herring propagation in scale and produces, and be easy to be accepted and promote.
Accompanying drawing explanation
Fig. 1 is that America hilsa herring parent promotes production the structural representation of circuit.
Embodiment
The present invention is made up of following production stage:
1, standby parent overwintering culture: artificial breeding is supported 2 age above America hilsa herring adult fish as the standby parent of artificial propagation, enter standby parent overwintering culture selecting healthy and strong, build grossness, body weight 0.75-1.5Kg commodity adult fish the annual 11-12 month, adopt simple and easy plastic tunnel cement pit to cultivate standby parent, adopt square or circular cement pit area to be 150-250m
2, dark 1.2-1.8m, pool wall is smooth; Arrange bow-shaped ring top above cement pit, top covers transparent heat insulating plastic film, shades membrane cover on a plastic film, light regulating by the rate of sheltering from heat or light 90%, reduces light transmittance, regulates the hiding rare of the film that shades simultaneously, generally maintain 30%-40% according to pond water water colour; Place gas head in cement pit, gas head density is 0.2-0.3/m
2, with Roots blower continuous charge in 24 hours, tolerance is with boiling-like.To cement pit sterilization before putting in a suitable place to breed, water inlet 20cm, full pool spilling head after dissolving with 20PPm bleaching powder, soak after 48 hours, draining is rinsed well, dries and re-uses for 3-4 days.When the standby parent selected puts in a suitable place to breed, adopt local fresh water, put latter one week in a suitable place to breed, when changing water at every turn, progressively improve salinity, rise salinity 2-3 ‰ at every turn, 12-15 ‰ is reached to last salinity, liter salt and overwintering culture water all adopt local salinity to be 10-15 ‰ estuarine water, useful water all adopts and precipitates through pond one-level, plastic tunnel cement pit nature sunshine pre-heating temperature elevation and two-stage precipitation, filter with 80 mesh sieve tulles again, change weekly water 1 time, each 2/3, every 25-35 days, turn over pond 1 time, overwintering culture water temperature remains on more than 8 DEG C, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C, bait is No. 3, commercial fresh-water fishes expanded pellet feed material, and crude protein content is 38%, throws something and feeds every day 1 time, and having ingested with 1-2 hour is as the criterion, cultivate pond and put Exopalaemon carinicauda in a suitable place to breed, stocking rate is 5-10 jin/pond,
2, parent's reinforced cultivating in spring: to by the end of March at the beginning of-4 months, enter parent's reinforced cultivating in spring stage, now in plastic tunnel cement pit, water temperature has just started to reach 15-16 DEG C, along with the rising of outside temperature, successively adopt plastic tunnel both sides to ventilate, remove plastic film, add a cover ground floor shade film, add a cover the second layer again and to shade film, keep water temperature 16-18 DEG C in cement pit; When changing water at every turn, progressively reduce salinity, each salt reduction degree 2-3 ‰, reaches local river to last salinity, salt reduction and reinforced cultivating water all adopt local river (fresh water), useful water all adopts through pond one-level precipitation, shade booth cement pit Temperature fall and two-stage precipitation, then filters with 80 mesh sieve tulles, and every 5-6 days changes water 1 time, each 2/3, turned over pond every 2 weeks 1 time, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C; Bait is No. 3, commercial fish expanded pellet feed material, and crude protein content is more than 40%, throws something and feeds every day 2 times, and having ingested with 1-2 hour is as the criterion; Other management are the same;
3, the parent that promotes production selects: at the beginning of-6 months mid-Mays, and the booth that now shades cultivates pool inner water temperature 17-19 DEG C, and parent raun belly expands successively, now can select and promote production with parent, criteria for selection: raun postabdomen expands and soft, and cloacal aperture is micro-red, and some gently presses belly to have ovum to flow out; Milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out, and chance water does not fall apart; The parent of select adopts drum (100L) to transport and puts into circuit of promoting production, put 2-3 tail parent for every barrel; Whole selecting wants soft not dried up with operation in transportation;
4, parent promotes production: circuit of promoting production (referring to Fig. 1) adopts rectangle cement pit (30.0m × 5.0m × 1.2m) 1, depth of water 1.0m, through constructing intermediate isolating wall 2 and smearing corner, round pond, form oval closed circuit (60.0m × 2.4m × 1.1m) 3, 0.75KW waterwheel aerator 4 is adopted to carry out oxygenation and form current 5, 10KW air source heat pump water heater group 6 is adopted to keep water temperature stability in circuit by cold and hot radiating tube 7, bow-shaped ring top is set above cement pit, top covers the film that shades of three layers of rate 90% of sheltering from heat or light, ensure that the intensity of illumination on daytime is lower than 300Lux, parent's breeding density of promoting production is 0.8 ~ 1 tail/m
2, sex ration is 1:1.1 ~ 1:1.3, and water temperature of promoting production is 17.5 ~ 19 DEG C,
5, natural spawning fertilization: after parent enters circuit of promoting production, keep absolutely quiet around, and adopt natural spawning fertilization mode, remaining closed circuit water velocity is 0.07 ~ 0.11m/s, and water temperature of promoting production is 17.5 ~ 19 DEG C; To promote production a small amount of expanding compound feed of throwing something and feeding in pond, residual bait will be removed in time;
6, fertilized egg is collected: America hilsa herring parent lays eggs mating time in the morning at day, general at 5:00-6:00, collect the general 7:00 of fertilized egg to start, before lower water is collected, suspend waterwheel aerator, after allowing flow rate of water flow lower, copy at the bottom of pond, place, circuit dead angle with 24 order soft bolting silk triangle diddle-net is flat, collect chalaza water operation, after copying ovum at every turn, open aerator 3-5 minute again, allow ovum automatic turning to circuit dead angle place, and then copy ovum, after repeating 3 times, then the ovum collecting of more than 95% can be cleared up, count after move on to hatchery.
Above-mentioned embodiment is through in the Technique of Raising Seeedling centre base test of this institute, and in 2014-2015 America hilsa herring parent culture with promote production, accumulatively obtain 17.76 ten thousand, fertilized egg, concrete condition is as follows:
Adopt hormone to hasten parturition parent on May 21st, 2014, May 22 and 23 days obtain ovum 110,000, fertilized egg 4.0 ten thousand, average fertilization rate 36.4%.
Divide 2 crowdes of America hilsa herring parents that promote production in May, 2015, amount to and obtain ovum 56.32 ten thousand, fertilized egg 13.76 ten thousand, average fertilization rate 24.4%.First, the parent that promotes production selecting for 2 ages on May 18 puts into east 10
#, female 64, male 76, sex ration is 1:1.2, and spawning and fertilization situation is in table 1; Second batch, the parent that promotes production selecting for 3 ages on May 28 puts into east 9
#, female 71, male 76, sex ration is 1:1.1, and spawning and fertilization situation is in table 2.
Table 12015 year first parent's spawning and fertilization information slip of promoting production (east 10
#)
Table 22015 year first parent's spawning and fertilization information slip of promoting production (east 9
#)
Claims (1)
1. one kind obtains the method for batch America hilsa herring fertilized egg, by standby parent overwintering culture, parent's reinforced cultivating in spring, the parent that promotes production selects, parent promotes production, natural spawning fertilization, fertilized egg collection production stage form, it is characterized in that artificial breeding to support 2 age above America hilsa herring adult fish as the standby parent of artificial propagation, enter standby parent overwintering culture selecting healthy and strong, build grossness, body weight 0.75-1.5Kg commodity adult fish the annual 11-12 month, adopt plastic tunnel cement pit cultivation standby parent, employing pool wall is smooth, area is 150-250m
2square or circular cement pit, dark 1.2-1.8m, bow-shaped ring top is set above cement pit, top covers transparent heat insulating plastic film, shade membrane cover on a plastic film, light regulating by the rate of sheltering from heat or light 90%, reduces light transmittance, regulate the hiding rare of the film that shades simultaneously according to pond water water colour, generally maintain 30%-40%, place gas head in cement pit, gas head density is 0.2-0.3/m
2, with Roots blower continuous charge in 24 hours, tolerance is with boiling-like, to cement pit sterilization before putting in a suitable place to breed, water inlet 20cm, full pool spilling head after dissolving with 20PPm bleaching powder, soak after 48 hours, draining is rinsed well, dries and re-uses for 3-4 days, when the standby parent selected puts in a suitable place to breed, adopt local fresh water, put latter one week in a suitable place to breed, when changing water at every turn, progressively improve salinity, rise salinity 2-3 ‰ at every turn, 12-15 ‰ is reached to last salinity, liter salt and overwintering culture water all adopt local salinity to be 10-15 ‰ estuarine water, useful water all adopts and precipitates through pond one-level, plastic tunnel cement pit nature sunshine pre-heating temperature elevation and two-stage precipitation, filter with 80 mesh sieve tulles again, change weekly water 1 time, each 2/3, every 25-35 days, turn over pond 1 time, overwintering culture water temperature remains on more than 8 DEG C, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C, bait is No. 3, commercial fresh-water fishes expanded pellet feed material, and crude protein content is 38%, throws something and feeds every day 1 time, and having ingested with 1-2 hour is as the criterion, cultivate excreta at the bottom of pond and the corrupt and polluted water of residual bait deposition to alleviate, put Exopalaemon carinicauda in a suitable place to breed in cultivation pond, stocking rate is 5-10 jin/pond, to by the end of March at the beginning of-4 months, enter parent's reinforced cultivating in spring stage, now in plastic tunnel cement pit, water temperature has just started to reach 15-16 DEG C, along with the rising of outside temperature, adopt plastic tunnel both sides to ventilate, remove plastic film, add a cover ground floor shade film, add a cover the second layer again and to shade film, keep water temperature 16-18 DEG C in cement pit, when changing water at every turn, progressively reduce salinity, each salt reduction degree 2-3 ‰, reaches local river to last salinity, salt reduction and reinforced cultivating water all adopt local river, useful water all adopts through pond one-level precipitation, shade booth cement pit Temperature fall and two-stage precipitation, then filters with 80 mesh sieve tulles, and every 5-6 days changes water 1 time, each 2/3, turned over pond every 2 weeks 1 time, change water temperature difference and be less than 2 DEG C, turn over pond water temperature difference and be less than 1 DEG C, bait is No. 3, commercial fish expanded pellet feed material, and crude protein content is more than 40%, throws something and feeds every day 2 times, and having ingested with 1-2 hour is as the criterion, other management are the same, at the beginning of-6 months mid-Mays, the booth that now shades cultivates pool inner water temperature 17-19 DEG C, and parent raun belly expands successively, now can select the use parent that promotes production, criteria for selection: raun postabdomen expands and soft, and cloacal aperture is micro-red, and some gently presses belly to have ovum to flow out, milter, light pressure cloacal aperture has a small amount of seminal fluid to flow out, and chance water does not fall apart, the parent of select adopts 100L drum to transport and puts into circuit of promoting production, put 2-3 tail parent for every barrel, whole selecting wants soft not dried up with operation in transportation, circuit of promoting production adopts 30.0m × 5.0m × 1.2m rectangle cement pit, depth of water 1.0m, through constructing intermediate isolating wall and smearing corner, round pond, form 60.0m × 2.4m × closed circuit of 1.1m ellipse, 0.75KW waterwheel aerator is adopted to carry out oxygenation and form current, 10KW air source heat pump water heater group is adopted to keep water temperature stability in circuit by cold and hot radiating tube, above cement pit is, bow-shaped ring top is set, top covers the film that shades of three layers of rate 90% of sheltering from heat or light, ensure that the intensity of illumination on daytime is lower than 300Lux, parent's breeding density of promoting production is 0.8 ~ 1 tail/m
2, sex ration is 1:1.1 ~ 1:1.3, and water temperature of promoting production is 17.5 ~ 19 DEG C, after parent enters circuit of promoting production, keep absolutely quiet around spawning inducing pool, adopt natural spawning fertilization mode, remaining closed circuit water velocity is 0.07 ~ 0.11m/s, and water temperature of promoting production is 17.5 ~ 19 DEG C, to promote production a small amount of expanding compound feed of throwing something and feeding in pond, absorb fertilized egg to reduce parent, but residual bait will be removed in time, otherwise expanded pellet diet is immersed in the time in water has grown and will sink to the bottom, be mixed into fertilized egg, cause fertilized egg to clean and removing impurities difficult, America hilsa herring parent has the custom of fertilized egg of ingesting, parent will collect fertilized egg after laying eggs in time, before collecting fertilized egg, suspend waterwheel aerator, after allowing flow rate of water flow lower, copy at the bottom of pond, place, circuit dead angle with 24 order soft bolting silk triangle diddle-net is flat, America hilsa herring egg membrane is very thin, and fertilized egg can not dried uply extrude, collecting ovum will be with water to operate, after copying ovum, then open aerator 3-5 minute at every turn, allow ovum automatic turning to circuit dead angle place, and then copy ovum, after repeating 3 times, then the ovum collecting of more than 95% can be cleared up, count after move on to hatchery.
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| CN105918165A (en) * | 2016-04-22 | 2016-09-07 | 苏州依科曼生物农业科技有限公司 | Overwintering method for aquaculture through brackish omni-directional flowing water |
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| CN108617555A (en) * | 2018-05-09 | 2018-10-09 | 上海市水产研究所 | A kind of method that recirculated water promotes America hilsa herring parent mating fertilization |
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| CN105918165A (en) * | 2016-04-22 | 2016-09-07 | 苏州依科曼生物农业科技有限公司 | Overwintering method for aquaculture through brackish omni-directional flowing water |
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| CN112640834A (en) * | 2020-12-25 | 2021-04-13 | 嘉鱼县罗家桥养殖专业合作社 | Device and method for increasing yield of leiocassis longirostris fry |
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