CN105284683A - Artificial hatching method for Saurogobio dabryi - Google Patents
Artificial hatching method for Saurogobio dabryi Download PDFInfo
- Publication number
- CN105284683A CN105284683A CN201510704880.5A CN201510704880A CN105284683A CN 105284683 A CN105284683 A CN 105284683A CN 201510704880 A CN201510704880 A CN 201510704880A CN 105284683 A CN105284683 A CN 105284683A
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- Prior art keywords
- water
- hatching
- minnow
- netting
- saurogobio
- Prior art date
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- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 27
- 230000012447 hatching Effects 0.000 title claims abstract description 22
- 241000643938 Saurogobio dabryi Species 0.000 title abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 32
- 235000013601 eggs Nutrition 0.000 claims abstract description 25
- 239000004568 cement Substances 0.000 claims abstract description 13
- 239000007921 spray Substances 0.000 claims abstract description 7
- 230000004720 fertilization Effects 0.000 claims abstract description 6
- 241000594009 Phoxinus phoxinus Species 0.000 claims description 43
- 238000011534 incubation Methods 0.000 claims description 23
- 210000004681 ovum Anatomy 0.000 claims description 11
- 102000002322 Egg Proteins Human genes 0.000 claims description 10
- 108010000912 Egg Proteins Proteins 0.000 claims description 10
- 241000270295 Serpentes Species 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 6
- 239000004744 fabric Substances 0.000 claims description 4
- 238000009826 distribution Methods 0.000 claims description 3
- 230000013020 embryo development Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000002504 physiological saline solution Substances 0.000 claims description 3
- 238000003892 spreading Methods 0.000 claims description 3
- 229910000831 Steel Inorganic materials 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000010959 steel Substances 0.000 claims description 2
- 230000018109 developmental process Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000005507 spraying Methods 0.000 abstract description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 6
- 229910052742 iron Inorganic materials 0.000 abstract 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- 230000003213 activating effect Effects 0.000 abstract 1
- 210000002257 embryonic structure Anatomy 0.000 abstract 1
- 230000017448 oviposition Effects 0.000 abstract 1
- 238000005070 sampling Methods 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 230000008774 maternal effect Effects 0.000 description 3
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 208000037805 labour Diseases 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 241000239250 Copepoda Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 210000000436 anus Anatomy 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial hatching method for Saurogobio dabryi. The method comprises the steps: (A) carrying out artificial dry fertilization after Saurogobio dabryi lays eggs, and activating sperms with 0.5% normal saline; (B) after fertilization, uniformly laying Saurogobio dabryi fertilized eggs on netting sheets, and suspending the laid netting sheets in a cement pool, wherein the depth of water of the cement pool is 48-52cm, and the distance from the netting sheets to the water surface is 5-10cm; (C) adopting a water-drenching hatching method, mounting a spraying water tap above the cement pool, and manually turning on a switch to spray water once every 28-32 minutes so as to humidify the fertilized eggs; and (D) taking off the netting sheets until eyespots appear during the development of fertilized egg embryos, and putting the netting sheets into a circulating-water hatching barrel, thereby completing hatching. The mesh of the netting sheets is 80 meshes; and a manufacturing method for the netting sheets comprises the steps of firstly, making a rectangular iron frame from iron wires, then, fixing a piece of netting on the iron frame, and tightening the netting, wherein the egg laying density is 15,000 per square meter. The method is feasible and is simple and convenient in operation, the hatchability is increased and can reach 80% or more. The technical problems in the artificial hatching and water mold control of Saurogobio dabryi are effectively solved.
Description
Technical field
The present invention relates to aquaculture field, particularly relate to the method for a kind of She Minnow artificial incubation, be also applicable to most hatching of producing viscid egg fish.
Background technology
She Minnow (Saurogobiodabryi) is under the jurisdiction of Cypriniformes, Cyprinidae, Minnow subfamily, She Minnow belongs to, and is a kind of small-sized economic fish, widely distributed in China, to the south until each water system of the Zhujiang River has She Minnow distributes from Heilungkiang.She Minnow is middle lower floor small fishes, with aquatic insect and copepoda for Major Foods.She Minnow mating season is the 3-5 month, ovum tool microviscosity, ovum footpath 1.0-1.1mm.She Minnow dressing percentage is high, stings little between flesh, and meat fertilizer is tender, delicious flavour, and nutritive value is higher, and the market price is high, demand is large.She Minnow is as the special breed variety of local name, and the exploitation being subject to the concern ， She Minnow industry of government and scientific and technical research personnel in recent years more and more comes into one's own.But, because the supply of the reason ， She Minnow such as catching intensity is excessive, environmental pollution cannot the amount of meeting the need of market.Therefore ， She Minnow propagate artificially imperative, the artificial propagation techniques of Dan She Minnow is not also captured completely, limits the development that She Minnow propagates industry artificially.Due to She Minnow mating season comparatively early, hatching water temperature is low, and cause its brooding time longer, therefore in its hatching process, fertilized egg very easily infects water mold, seriously reduces its incubation rate.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, is the method that there are provided a kind of She Minnow artificial incubation, easy to implement the method, easy and simple to handle, and improve incubation rate, incubation rate can reach more than 80%.Effectively solve the technical barrier of She Minnow artificial incubation and water mold control.
In order to realize above-mentioned object, the present invention adopts following technical measures:
A method for She Minnow artificial incubation, the steps include:
A, She Minnow adopts artificial dry method to inseminate after laying eggs, activate sperm with the physiological saline of 0.5% (mass volume ratio).
B, after fertilization are Jiang She Minnow fertilized egg uniform spreading in mesh sheet, and the mesh sheet completed is suspended in cement pit, cement pit depth of water 48-52cm, mesh sheet and water surface distance 5-10cm.
C, the method (editing " aqiuculrue " chapter 4 with reference to Li Jiale) adopting trickle to hatch, common spray water faucet is housed above cement pit, and every 28-32 minute, manually opened switch water spray once, guaranteed that fertilized egg is moistening.
Mesh sheet, until fertilized egg embryonic development occurs to eyespot, is now taken off by D, puts into recirculated water hatching bucket, completes hatching.
Described mesh sheet mesh is 80 orders, and preparation method is first make the chase of a rectangle with steel wire, then is fixed on chase by screen cloth, requires that screen cloth is tight, is convenient to spread ovum, and paving ovum density is 15000 every square metre.
Described liquid distribution (common) is manually opened spray water faucet, and arranging interval time is 28-32 minute, and each trickle time is 4-6min.
Described recirculated water hatching bucket is that (market is purchased, and arrange flow velocity before and after common) ， She Minnow fertilized egg membrane is about 0.2m/s to traditional incubation equipment, and after membrane completes, adjustment flow velocity is 0.1m/s.Spawning water require water quality pure and fresh, pollution-free, without large granular impurities such as silts, without harmful organisms.
Adopt this method can effective the growing of water mold in control She Minnow incubating oosperm process, improve incubation rate, this method incubation rate can reach more than 80%.
The different water temperature of table 1 is Yu the relation of She Minnow brooding time
Compared with prior art, the invention has the beneficial effects as follows:
She Minnow artificial fecundation method provided by the invention, improve traditional hatching m, convenient operation, She Minnow incubating oosperm time long, the lower water mold caused of water temperature is prevented to grow in a large number with non-drug means, the method hatching effect is good, can improve the incubation rate of She Minnow fertilized egg, the development propagating industry Wei She Minnow artificially is laid a good foundation.Improve incubation rate, incubation rate reaches more than 80%.
Embodiment
Embodiment 1: the technological means specifically setting forth the present invention's realization below in conjunction with example.
A method for She Minnow artificial incubation, the steps include:
A, morning April 8 draw in the net to obtain snake Minnow parent, select 49 tails maternal, 10 tail male parents.Evening, 21:00 point, started artificial induced spawning.
B, afternoon April 9 13:00, female snake Minnow parent starts to lay eggs, and 13:30 is laid eggs end, adopts artificial dry method to inseminate, is fertilized with the physiological saline of 0.5% (mass volume ratio).43 tail raun natural labors, spawning rate 87.8%, obtains ovum amount 31000 altogether, and evening April 9,23:00 point checked that fertilization rate is 79.2%.
C, be fertilized after ovum rapidly, be evenly taped against in mesh sheet, mesh sheet size is 80cm × 40cm, the ovum of about 5000 on each mesh sheet uniform spreading.The mesh sheet quick suspension completed in cement pit, mesh sheet and water surface distance 5-10cm.
After D, paving ovum complete, with plastic film, cement pit is covered, to improve temperature and maintenance air humidity in cement pit, guarantee the moisture needed for development of fertilized ova.To monitor in cement pit temperature every day 3 times, between the incubation period, mean temperature of air is 24 DEG C.
E, unlatching automatic water spraying sprayer unit, arranging interval time is 28 or 29 or 30 or 31 or 32 minutes, each duration 5min.
Regularly use F, every day microscopic examination fertilized egg embryonic development situation, when occurring to eyespot, mesh sheet is proceeded in hatching pail and completes hatching.
Morning April 11 5:00, microscopy finds that eyespot has all appearred in most fertilized egg, now mesh sheet is proceeded to immediately in hatching pail, and adjustment flow velocity is about 0.2m/s.
Morning April 11 11:00, snake Minnow prelarva starts membrane, and snake Minnow newly hatched larvae whole body is transparent, and chest has an obvious yolk, rounded, successively extends to anus place.To about 18:00 in afternoon, membrane completes substantially, and flow velocity is adjusted to about 0.1m/s.
Random sampling estimation hatches She Minnow prelarva 20000 tail altogether, and calculating incubation rate is 81.5%.
About 17:00, manually fished for zooplankton and threw something and fed in hatching pail afternoon April 14, and 19:00 random sampling microscopy finds the snake Minnow prelarva initial feeding all of more than 80%.
About 8:00, threw in snake Minnow prelarva in pond, entered the seed rearing stage morning April 15.
Embodiment 2:
A method for snake Minnow artificial incubation, the steps include: (applicant in 2015 add carried out the production of mass on a large scale, general effect is good)
Some is different for its step of A and embodiment 1, and applicant, by the time advance of breeding, started breeding March 20, have chosen 536 tails altogether maternal, 96 tail male parents, and the same day, 21:00 point, started artificial induced spawning.
B the next afternoon about 17:00, parent starts to lay eggs, and method of laying eggs, Insemination procedures, fertilized egg liquid distribution are all identical with embodiment 1.The maternal natural labor of totally 472 tails of random sampling statistics, spawning rate 88.1%, 330,000, common property ovum, secondary daily inspection fertilization rate 83.0%, fertilized egg amount is about 27.4 ten thousand.Mean temperature of air 19 DEG C between the incubation period.
The afternoon C3 month 24, about 14:00 was hatched, and random sampling measuring and calculating hatches She Minnow prelarva 22.8 ten thousand tail, incubation rate 83.2% altogether.
D is compared with embodiment 1, and be advanced by repoductive time and the significantly prolongation of brooding time, production cycle can be caused longer, but the snake Minnow parent maturity in this season is relatively better, spawning rate, incubation rate are all higher.Should take the circumstances into consideration aborning to consider this 2 point, reasonable coordination, to enhance productivity.
Claims (1)
1. a method for snake Minnow artificial incubation, the steps include:
She Minnow adopts artificial dry method to inseminate after laying eggs, activate sperm with the physiological saline of 0.5% mass volume ratio;
After fertilization is Jiang She Minnow fertilized egg uniform spreading in mesh sheet, and the mesh sheet completed is suspended in cement pit, cement pit depth of water 48-52cm, mesh sheet and water surface distance 5-10cm;
Adopt the method for trickle hatching, above cement pit, spray water faucet is housed, every 28-32 minute, once, fertilized egg was moistening for manually opened switch water spray;
Until fertilized egg embryonic development occurs to eyespot, now mesh sheet is taken off, put into recirculated water hatching bucket, complete hatching;
Described mesh sheet mesh is 80 orders, and preparation method is first make the chase of a rectangle with steel wire, then is fixed on chase by screen cloth, and screen cloth is tight, and paving ovum density is 15000 every square metre;
Described liquid distribution is manually opened spray water faucet, and arranging interval time is 28-32 minute, and each trickle time is 4-6min;
Described recirculated water hatching bucket is that to arrange flow velocity before and after incubation equipment ， She Minnow fertilized egg membrane be 0.2m/s, and after membrane completes, adjustment flow velocity is 0.1m/s.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510704880.5A CN105284683A (en) | 2015-10-26 | 2015-10-26 | Artificial hatching method for Saurogobio dabryi |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510704880.5A CN105284683A (en) | 2015-10-26 | 2015-10-26 | Artificial hatching method for Saurogobio dabryi |
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| Publication Number | Publication Date |
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| CN105284683A true CN105284683A (en) | 2016-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510704880.5A Pending CN105284683A (en) | 2015-10-26 | 2015-10-26 | Artificial hatching method for Saurogobio dabryi |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172131A (en) * | 2016-07-21 | 2016-12-07 | 山东省海洋生物研究院 | A kind of method of recirculated water linked network sheet hatching sea water green grass or young crops germ cell |
| CN114557297A (en) * | 2022-03-10 | 2022-05-31 | 天门粤海饲料有限公司 | Method for artificially hatching micropterus salmoides fertilized eggs |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1231295A (en) * | 1968-09-26 | 1971-05-12 | ||
| CN201135078Y (en) * | 2007-12-29 | 2008-10-22 | 华中农业大学 | A water saving and mould proof hatching device suitable for yellow catfish fertilized ovum |
| CN201365454Y (en) * | 2009-02-20 | 2009-12-23 | 江苏省淡水水产研究所 | Fry incubator for directional mating of channel catfish |
| CN103960188A (en) * | 2014-05-20 | 2014-08-06 | 中国水产科学研究院长江水产研究所 | Artificial spawning induction method for rhinogobio ventralis |
| CN203897035U (en) * | 2014-01-27 | 2014-10-29 | 北京市通州鑫淼水产总公司 | Adhesive roe distributing device |
-
2015
- 2015-10-26 CN CN201510704880.5A patent/CN105284683A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1231295A (en) * | 1968-09-26 | 1971-05-12 | ||
| CN201135078Y (en) * | 2007-12-29 | 2008-10-22 | 华中农业大学 | A water saving and mould proof hatching device suitable for yellow catfish fertilized ovum |
| CN201365454Y (en) * | 2009-02-20 | 2009-12-23 | 江苏省淡水水产研究所 | Fry incubator for directional mating of channel catfish |
| CN203897035U (en) * | 2014-01-27 | 2014-10-29 | 北京市通州鑫淼水产总公司 | Adhesive roe distributing device |
| CN103960188A (en) * | 2014-05-20 | 2014-08-06 | 中国水产科学研究院长江水产研究所 | Artificial spawning induction method for rhinogobio ventralis |
Non-Patent Citations (2)
| Title |
|---|
| 梁书文 张卫宪: "《宠物繁殖》", 31 August 2008, 中国农业科学技术出版社 * |
| 王建新: "《鱼虾苗种培育新技术》", 31 January 1999, 中国农业出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106172131A (en) * | 2016-07-21 | 2016-12-07 | 山东省海洋生物研究院 | A kind of method of recirculated water linked network sheet hatching sea water green grass or young crops germ cell |
| CN114557297A (en) * | 2022-03-10 | 2022-05-31 | 天门粤海饲料有限公司 | Method for artificially hatching micropterus salmoides fertilized eggs |
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Application publication date: 20160203 |
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