CN111567450A - Artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum - Google Patents
Artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Download PDFInfo
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- CN111567450A CN111567450A CN202010399509.3A CN202010399509A CN111567450A CN 111567450 A CN111567450 A CN 111567450A CN 202010399509 A CN202010399509 A CN 202010399509A CN 111567450 A CN111567450 A CN 111567450A
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- 241000746747 Glyptosternon maculatum Species 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000011218 seed culture Methods 0.000 title claims abstract description 12
- 241000251468 Actinopterygii Species 0.000 claims abstract description 60
- 238000011534 incubation Methods 0.000 claims abstract description 19
- 230000009027 insemination Effects 0.000 claims abstract description 10
- 238000009395 breeding Methods 0.000 claims abstract description 4
- 230000001488 breeding effect Effects 0.000 claims abstract description 4
- 230000032696 parturition Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 55
- 230000012447 hatching Effects 0.000 claims description 17
- 239000007924 injection Substances 0.000 claims description 17
- 238000002347 injection Methods 0.000 claims description 17
- 102000002322 Egg Proteins Human genes 0.000 claims description 15
- 108010000912 Egg Proteins Proteins 0.000 claims description 15
- 235000013601 eggs Nutrition 0.000 claims description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 210000002969 egg yolk Anatomy 0.000 claims description 9
- 235000013345 egg yolk Nutrition 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 210000001015 abdomen Anatomy 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 230000006698 induction Effects 0.000 claims description 6
- 210000004681 ovum Anatomy 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 5
- 230000000366 juvenile effect Effects 0.000 claims description 4
- 241000256128 Chironomus <genus> Species 0.000 claims description 3
- 241000238571 Cladocera Species 0.000 claims description 3
- 241001290772 Limnodrilus Species 0.000 claims description 3
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims description 3
- 101800000989 Oxytocin Proteins 0.000 claims description 3
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims description 3
- 208000027418 Wounds and injury Diseases 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- 210000003746 feather Anatomy 0.000 claims description 3
- 210000004392 genitalia Anatomy 0.000 claims description 3
- 210000002149 gonad Anatomy 0.000 claims description 3
- 230000036449 good health Effects 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 230000001418 larval effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000004570 mortar (masonry) Substances 0.000 claims description 3
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims description 3
- 229960001723 oxytocin Drugs 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 230000008961 swelling Effects 0.000 claims description 3
- 239000008399 tap water Substances 0.000 claims description 3
- 235000020679 tap water Nutrition 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 2
- 239000011152 fibreglass Substances 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000009472 formulation Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002504 physiological saline solution Substances 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 4
- 230000002180 anti-stress Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 230000008439 repair process Effects 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract 1
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 3
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 3
- 241000746746 Glyptosternon Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 229940015047 chorionic gonadotropin Drugs 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 241001233037 catfish Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000002434 gonadorelin derivative Substances 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 235000013550 pizza Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
An artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regan belongs to the technical field of fish culture. The technology breaks through the bottleneck that the wild seed quality of Glyptosternum maculatum Regans is scarce to cause difficulty in the source of parent fishes, provides technical support and seed guarantee for the wild resource repair of Glyptosternum maculatum Regans, and can realize the large-scale seed cultivation of Glyptosternum maculatum Regans. The method comprises the following steps: step (1): selecting parent Glyptosternum maculatum; step (2): artificial hasten parturition; and (3): artificial insemination; and (4): artificial incubation; and (5): cultivating seedlings; 5.1 open cultivation; and 5.2, breeding the fry. The method is used for artificial induced spawning and low-temperature seed culture of the Glyptosternum maculatum Regans, and greatly improves the survival rate, the fertilization rate and the hatchability of wild parent Glyptosternum maculatum Regans. By low-temperature cultivation and feeding of nutrient baits at different stages, the fry quality of Glyptosternum maculatum Regans is improved, the anti-stress capability of fries is enhanced, the survival rate of the fries is greatly improved, and the requirement of fry resource proliferation is met.
Description
Technical Field
The invention relates to an artificial induced spawning and seed culture method of Glyptosternum maculatum Regan, and belongs to the technical field of fish culture.
Background
Glyptosternum maculatum (Glyptosternnum maculatum) belongs to Glyptosternum of Glyptosternum family, Glyptosternum genus, commonly known as Pakri and Lasatay catfish, is a special fish in middle and upstream of Bujiang Yalu Tibetan, and has a unique biological evolution mechanism suitable for plateau water areas. The Glyptosternum maculatum Regan has tender meat, delicious taste and few muscle thorns, has certain medicinal value and is deeply loved and advocated by people in the Tibetan region. Currently, the sale price in the pizza market is as high as 1700-2600 RMB/kg. In recent years, under the influence of over-fishing, hydraulic engineering, environmental pollution, habitat change and the like, the population resource quantity of Glyptosternum maculatum is continuously reduced, the fishing quantity cannot be formed, and 56 extremely-dangerous (CR) fish records of China biodiversity Red records-vertebrate rolls are listed in 2015. Therefore, the development of artificial propagation, culture, utilization and protection research of Glyptosternum maculatum Regans has important significance for recovering the natural resource amount of Glyptosternum maculatum Regans. So far, no research report on artificial induced spawning and low-temperature seed culture methods of Glyptosternum maculatum Regan exists at home and abroad.
Disclosure of Invention
The invention aims to provide an artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum. The technology breaks through the bottleneck that the wild seed quality of Glyptosternum maculatum Regans is scarce to cause difficulty in the source of parent fishes, provides technical support and seed guarantee for the wild resource repair of Glyptosternum maculatum Regans, and can realize the large-scale seed cultivation of Glyptosternum maculatum Regans.
The technical scheme of the invention is as follows:
an artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regn, comprising the following steps:
step (1): selecting parent fish; selecting high-quality wild Glyptosternum maculatum Regans with body mass of more than 50g, body length of more than 14cm, no injury on body surface, good health and activity, plump abdomen, and mature gonad, placing in a running water hatching pond, and culturing at water temperature of 12 deg.C;
step (2): artificial hasten parturition; the artificial spawning induction of female fish adopts a secondary injection method, and the artificial spawning induction of male fish adopts a primary injection method; the oxytocin adopts LRH-A2+ DOM + HCG cocktail formulation; the injection dosage of the first needle of the female fish is 4ug LRH-A2+2mgDOM +600 IUHCG; injecting a second needle 12 hours later, wherein the injection dose is 2ug LRH-A2+1mg DOM +300 IUHCG; injecting the male fish and the female fish together by using a second needle, wherein the injection dosage is 1/2 of the total injection dosage of the female fish; the artificial induced spawning water temperature is 12.5-13 ℃, and the effect time is 10-14 h; the ratio of the tail number of the male fish to the tail number of the female fish is 1:2, after eggs of the female fish are dissociated, the spermary of the male fish is taken out, weighed, added with 2 times of weight of physiological saline and evenly crushed by a mortar; at the moment, fishing out the female fish, wiping the genital hole with a dry towel, fixing the female fish body with one hand, and lightly pressing the abdomen with the other hand to discharge the eggs into a wiped stainless steel basin;
and (3): artificial insemination; adopting a dry insemination mode for artificial insemination, pouring the crushed spermary tissue into a stainless steel basin containing ovum grains, stirring for 5-6min by using feathers, adding clear water at 13 ℃, continuously stirring for 3-5min, pouring out the stirring liquid, adding clear water, and cleaning the fertilized ovum for 3-5 times;
and (4): artificial incubation; uniformly arranging the fertilized eggs after water swelling in an incubation nest, wherein the incubation water temperature is 13-14 ℃, and the larvae are taken out of the membrane after 120-150 h; ensuring the dissolved oxygen of the hatching water to be more than 6mg/l in the hatching process; the incubation mode adopts micro-flow water incubation or micro-flow water inflation incubation;
and (5): cultivating seedlings;
5.1 open cultivation; after fertilized eggs are incubated for 3-5 days, artificial domestication is carried out by adopting a method of feeding egg yolk water, 20 g of cooked egg yolk is mixed with 2kg of tap water, the egg yolk water after filtration by gauze is fed with 3000-flavored fry for 4 times a day, the egg yolk water is fed for 10-15 days, then the water flea is fed for 4 times a day, the feeding amount is 10 percent of the total weight of the fry, the fry is placed in a culture tank for water culture after 30-40 days, the culture water temperature is 11-12 ℃, and the dissolved oxygen in the water is more than 6 mg/l;
5.2 fry breeding; feeding limnodrilus giganteus for 4 times every day after hatching for 70 days, wherein the feeding amount is 15% of the total weight of the larval fish each time, and the feeding time is 90-120 days; then, feeding chironomus larvae until the size of juvenile fish reaches more than 2-3 cm; feeding 3 times a day, wherein the feeding amount is 5-10% of the total weight of young fish, culturing in running water at 11-12 deg.C and water dissolved oxygen above 6 mg/l.
Compared with the prior art, the invention has the beneficial effects that: according to the life habits of the Glyptosternum maculatum Regan, the invention provides a good cultivation environment and a cultivation method for the parent fish of the Glyptosternum maculatum Regan, and greatly improves the survival rate, the fertility rate and the hatchability of the wild parent fish of the Glyptosternum maculatum Regan; by low-temperature cultivation and feeding of nutrient baits at different stages, the fry quality of Glyptosternum maculatum Regans is improved, the anti-stress capability of fries is enhanced, the survival rate of the fries is greatly improved, and the requirement of fry resource proliferation is met.
Detailed Description
The first embodiment is as follows: the embodiment discloses an artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regan, which comprises the following steps:
step (1): selecting parent fish; selecting high-quality wild Glyptosternum maculatum Regans with body mass of more than 50g, body length of more than 14cm, no injury on body surface, good health and activity, plump abdomen, and mature gonad, placing in a running water hatching pond, and culturing at water temperature of 12 deg.C;
step (2): artificial hasten parturition; the artificial spawning induction of female fish adopts a secondary injection method, and the artificial spawning induction of male fish adopts a primary injection method; the oxytocin adopts LRH-A2(luteinizing hormone releasing hormone analog) + DOM (diutanone maleate) + HCG (chorionic gonadotropin) cocktail; the injection dosage of the first needle of the female fish is 4ug LRH-A2+2mgDOM +600 IUHCG; injecting a second needle 12 hours later, wherein the injection dose is 2ug LRH-A2+1mgDOM +300 IUHCG; 1/2 injecting male fish and female fish together with the second needle, wherein the injection dosage is 1/2 of the total injection dosage (total dosage of the first needle and the second needle) of the female fish; the artificial induced spawning water temperature is 12.5-13 ℃, and the effect time is 10-14 h; the ratio of the tail number of the male fish to the tail number of the female fish is 1:2, after eggs of the female fish are dissociated, the spermary of the male fish is taken out, weighed, added with 2 times of body weight of normal saline (0.65% NaCl) and evenly crushed by a mortar; at the moment, fishing out the female fish, wiping the genital hole with a dry towel, fixing the female fish body with one hand, and lightly pressing the abdomen with the other hand to discharge the eggs into a wiped stainless steel basin;
and (3): artificial insemination; adopting a dry insemination mode for artificial insemination, pouring the crushed spermary tissue into a stainless steel basin containing ovum grains, stirring for 5-6min by using feathers, adding clear water at 13 ℃ (the adding amount is not limited), continuously stirring for 3-5min, pouring out the stirring liquid, adding clear water, and cleaning the fertilized ovum for 3-5 times;
and (4): artificial incubation; uniformly arranging the fertilized eggs after water swelling in an incubation nest, wherein the incubation water temperature is 13-14 ℃, and the larvae are taken out of the membrane after 120-150 h; ensuring the dissolved oxygen of the hatching water to be more than 6mg/l in the hatching process; the incubation mode adopts micro-flow water incubation or micro-flow water inflation incubation;
and (5): cultivating seedlings;
5.1 open cultivation; after fertilized eggs are incubated for 3-5 days, artificial domestication is carried out by adopting a method of feeding egg yellow water, 20 g of cooked egg yolk is mixed with 2kg of tap water, 2000-plus-3000-fish fries are fed with the egg yellow water after being filtered by gauze, the fish fries are fed for 4 times a day (the feeding time is 8:00, 12:00, 4:00 and 20:00 respectively), the egg yellow water is fed for 10-15 days, then the fish fries are fed by small water fleas for 4 times a day (the feeding time is 8:00, 12:00, 4:00 and 20:00 respectively), the feeding amount is 10 percent of the total weight of the fish fries (the feeding time is 6:00, 12:00, 18:00 and 24:00 respectively), the fish fries are placed in a culture tank for water culture after being fed for 30-40 days, the water culture temperature is 11-12 ℃, and the dissolved oxygen in water is more than 6 mg/l;
5.2 fry breeding; feeding limnodrilus giganteus 70 days after hatching for 4 times a day (the feeding time is 6:00, 12:00, 18:00 and 24:00 respectively), wherein the feeding amount of each time is 15% of the total weight of the larval fish, and the feeding time is 90-120 days; then, feeding chironomus larvae until the size of juvenile fish reaches more than 2-3 cm; feeding 3 times a day (feeding time is 8:00, 14:00 and 20:00 respectively), wherein the feeding amount is 5-10% of the total weight of the juvenile fish, the water temperature is 11-12 deg.C, and the water dissolved oxygen is above 6 mg/l.
Further, in the step (1), the hatching tank is circular, and the diameter of the inner circumferential surface of the hatching tank is 1.2m and the height of the inner circumferential surface of the hatching tank is 0.5 m.
Further, in the step (5) 5.1, the cultivation tank adopts a long-strip glass fiber reinforced plastic cultivation tank, the cultivation tank is 2-3m long, 50cm wide and 40cm high.
Claims (3)
1. An artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regan is characterized in that: the method comprises the following steps:
step (1): selecting parent fish; selecting high-quality wild Glyptosternum maculatum Regans with body mass of more than 50g, body length of more than 14cm, no injury on body surface, good health and activity, plump abdomen, and mature gonad, placing in a running water hatching pond, and culturing at water temperature of 12 deg.C;
step (2): artificial hasten parturition; the artificial spawning induction of female fish adopts a secondary injection method, and the artificial spawning induction of male fish adopts a primary injection method; the oxytocin adopts LRH-A2+ DOM + HCG cocktail formulation; the injection dosage of the first needle of the female fish is 4ug LRH-A2+2mg DOM +600IU HCG; after 12 hours a second needle was injected at a dose of 2ug LRH-A2+1mg DOM +300IU HCG; injecting the male fish and the female fish together by using a second needle, wherein the injection dosage is 1/2 of the total injection dosage of the female fish; the artificial induced spawning water temperature is 12.5-13 ℃, and the effect time is 10-14 h; the ratio of the tail number of the male fish to the tail number of the female fish is 1:2, after eggs of the female fish are dissociated, the spermary of the male fish is taken out, weighed, added with 2 times of weight of physiological saline and evenly crushed by a mortar; at the moment, fishing out the female fish, wiping the genital hole with a dry towel, fixing the female fish body with one hand, and lightly pressing the abdomen with the other hand to discharge the eggs into a wiped stainless steel basin;
and (3): artificial insemination; adopting a dry insemination mode for artificial insemination, pouring the crushed spermary tissue into a stainless steel basin containing ovum grains, stirring for 5-6min by using feathers, adding clear water at 13 ℃, continuously stirring for 3-5min, pouring out the stirring liquid, adding clear water, and cleaning the fertilized ovum for 3-5 times;
and (4): artificial incubation; uniformly arranging the fertilized eggs after water swelling in an incubation nest, wherein the incubation water temperature is 13-14 ℃, and the larvae are taken out of the membrane after 120-150 h; ensuring the dissolved oxygen of the hatching water to be more than 6mg/l in the hatching process; the incubation mode adopts micro-flow water incubation or micro-flow water inflation incubation;
and (5): cultivating seedlings;
5.1 open cultivation; after fertilized eggs are incubated for 3-5 days, artificial domestication is carried out by adopting a method of feeding egg yolk water, 20 g of cooked egg yolk is mixed with 2kg of tap water, the egg yolk water after filtration by gauze is fed with 3000-flavored fry for 4 times a day, the egg yolk water is fed for 10-15 days, then the water flea is fed for 4 times a day, the feeding amount is 10 percent of the total weight of the fry, the fry is placed in a culture tank for water culture after 30-40 days, the culture water temperature is 11-12 ℃, and the dissolved oxygen in the water is more than 6 mg/l;
5.2 fry breeding; feeding limnodrilus giganteus for 4 times every day after hatching for 70 days, wherein the feeding amount is 15% of the total weight of the larval fish each time, and the feeding time is 90-120 days; then, feeding chironomus larvae until the size of juvenile fish reaches more than 2-3 cm; feeding 3 times a day, wherein the feeding amount is 5-10% of the total weight of young fish, culturing in running water at 11-12 deg.C and water dissolved oxygen above 6 mg/l.
2. The artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regans according to claim 1, which is characterized in that: in the step (1), the hatching pond is circular, and the diameter of the circumferential surface in the hatching pond is 1.2m and the height is 0.5 m.
3. The artificial induced spawning and low-temperature seed culture method of Glyptosternum maculatum Regans according to claim 1, which is characterized in that: in the 5.1 of the step (5), the cultivation tank adopts a long-strip glass fiber reinforced plastic cultivation tank, the length of the cultivation tank is 2-3m, the width of the cultivation tank is 50cm, and the height of the cultivation tank is 40 cm.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112021216A (en) * | 2020-09-27 | 2020-12-04 | 西藏自治区农牧科学院水产科学研究所 | Mixed culture method of Glyptosternum maculatum |
| CN112772470A (en) * | 2020-12-31 | 2021-05-11 | 中国水产科学研究院黑龙江水产研究所 | Artificial cultivation method of juvenile glyptosternum maculatum |
| CN116998426A (en) * | 2023-07-18 | 2023-11-07 | 西藏自治区农牧科学院水产科学研究所 | Method for collecting wild parent fish of raw red-mackerel |
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