KR100902406B1 - Feed for ell - Google Patents

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KR100902406B1
KR100902406B1 KR1020080016478A KR20080016478A KR100902406B1 KR 100902406 B1 KR100902406 B1 KR 100902406B1 KR 1020080016478 A KR1020080016478 A KR 1020080016478A KR 20080016478 A KR20080016478 A KR 20080016478A KR 100902406 B1 KR100902406 B1 KR 100902406B1
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weight
mulberry
feed
grams
eel
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KR20080079600A (en
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최영생
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최영생
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

본 발명은 장어양식용 사료에 관한 것으로서, 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 100 중량부에 대해 사료첨가제가 0. 1 내지 0.2 중량부로 첨가되어 있고, 상기 사료 첨가제는 수분함량이 15 내지 20%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램을 혼합한 혼합물에 대해 5 내지 7배 중량의 물에 투입하여 85 내지 90℃에서 저온 열추출한 추출액을 침전시킨 후 여과처리한 여과추출액을 가열하여 300cc로 농축하여 형성된다. 이러한 장어 양식용 사료에 의하면, 양식과정에서 장어의 면역성이 향상되어 폐사율이 현저하게 감소하여 항생제의 사용을 억제할 수 있고, 상처발생 예방 빠른 치유에 의해 외관상 상처흔적이 거의 없으며, 성장효율성도 높일 수 있는 장점을 제공한다.The present invention relates to a feed for eel culture, consisting of 49% by weight crude protein, 3% by weight crude fat, 2% by weight calcium, 2.7% by weight phosphorus, 3% by weight crude fiber, 18% by weight ash, 22.3% by weight squid meat A feed additive is added in an amount of 0.01 to 0.2 parts by weight based on 100 parts by weight of the feed, and the feed additive is mixed with mulberry leaves, mulberry branches, and mulberry roots, which are dried and dried to have a water content of 15 to 20% in the same weight ratio. 360 grams of mulberry, 270 grams of lotus leaf and lotus root, cut to dry at 15 to 20% moisture content, and 380 grams of ivy chopped to dry at 15 to 20% moisture content. The mixture was mixed with 5 to 7 times the weight of water and precipitated by extracting the low temperature heat extraction at 85 to 90 ° C. The filtrate was filtered and concentrated to 300cc. According to the feed for eel culture, the immunity of the eel is improved during the aquaculture process, which significantly reduces the mortality rate, thereby suppressing the use of antibiotics. It provides the advantages.

장어, 양식, 사료 Eel, farmed, forage

Description

장어양식용 사료{feed for ell}Feed for eel {feed for ell}

본 발명은 장어 양식용 사료에 관한 것이다.The present invention relates to feed for eel farming.

최근에는 수산물이 건강식품이라는 인식이 확산됨에 따라 수산물의 소비가 증가하고 있으며 이에 따라 어류 양식 또한 양적으로 증가하고 있다.Recently, as the awareness that seafood is a health food spread, the consumption of aquatic products has increased, and fish farming has also increased in quantity.

장어는 고단백 스테미너 식품으로, 비타민 A, B, E가 많이 함유되어 있다. 또한, 장어의 지방에는 DHA 1332mg과 EPA 742mg 그리고 레시친(Lecithin)이 풍부하게 포함되어 있는데 레시친은 인체의 세포막을 구성하는 주성분으로 뇌세포에는 없어서는 안 될 귀중한 물질이다. Eel is a high protein stamina food and contains a lot of vitamins A, B and E. In addition, eel fat contains abundant DHA 1332mg, EPA 742mg and lecithin. Lecithin is the main component of the human cell membrane and is indispensable for brain cells.

장어는 성장단계가 여러 단계로 나뉘어져 있으며 인공산란, 부화를 할 수 없는 특이 종으로 그 생리적 특성 때문에 성장 중간단계인 실뱀장어를 체포하여 적당한 환경에서 양식해야 하는데, 실뱀장어에서 식용장어까지의 일반적 양식과정에서 대량밀집 형태의 양식으로 인한 세균성 질병 및 기생충성 질병 등 각종 감염성 질병이 발병하여 양식장어가 집단 폐사 하는 사례가 많다. The eel is divided into several stages of growth, and it is a unique species that cannot artificially spawn and hatch. Because of its physiological characteristics, eel must be caught in the middle stage of growth and cultured in a suitable environment. In the process, various infectious diseases such as bacterial diseases and parasitic diseases due to the mass density of aquaculture are caused, and many cases of farmed eel die.

이를 막기 위해 양식현장에서 질병예방이나 치료목적으로 항생제를 사료에 첨가하거나 양식장의 물에 첨가하여 사용하고 있으나, 이로 인해 식품안정성이 떨 어지고 내병성도 나빠지는 문제점을 야기시키고 있다. 최근에는 이러한 문제점을 개선하고자 양식 대상 어류에 적합하면서도 면역능을 증강시킬 수 있는 천연 물질에 대한 연구가 꾸준히 진행되고 있으나 아직까지는 적용 어류에 적합한 물질에 대한 개발은 미비한 형편이다. In order to prevent this, antibiotics are added to feed or water for farming in order to prevent or treat diseases. However, this causes a problem of deteriorating food stability and worsening disease resistance. In recent years, researches on natural substances that can enhance immunity while being suitable for aquaculture fish have been steadily progressed to improve these problems. However, the development of suitable substances for applied fish is still insufficient.

한편, 등록특허 제 0585500호에는 양식 민물장어 사료첨가제 및 이것을 포함하는 양식 민물장어 사료가 개시되어 있다.On the other hand, Patent No. 0585500 discloses aquaculture freshwater eel feed additives and aquaculture freshwater eel feed comprising the same.

개시된 양식 민물장어 사료 첨가제는 키토산에 영지버섯 추출액을 첨가한 혼합물을 가수분해하여 얻어진 혼합물 키토산올리고당 유도체로 이루어진다. 그러나 이러한 첨가제는 면역성은 높일 수 있으나, 밀집 사육되는 과정에서 상호 접촉에 의해 발생될 수 있는 외상에 대한 치료 효과가 적고, 상대적으로 가격이 비싸 대량 생산용 사료첨가제로서는 적합하지 않은 단점이 있다.The disclosed aquaculture freshwater eel feed additive consists of a mixture chitosan oligosaccharide derivative obtained by hydrolyzing a mixture of Ganoderma lucidum extract added to chitosan. However, these additives can increase the immunity, but there is a disadvantage that the therapeutic effect against trauma that may be caused by mutual contact in the process of dense breeding is relatively low, and is not suitable as a mass additive feed additive.

본 발명은 상술한 바와 같은 문제점을 해결하기 위한 것으로, 장어의 면역능력과 항병력을 향상시킬 수 있으면서 밀집 사육에 의해 발생될 수 있는 외상에 대한 치유효과를 높일 수 있는 장어 양식용 사료 첨가제와 이를 함유한 장어 양식 사료를 제공함에 그 목적이 있다.The present invention is to solve the problems as described above, while containing the additives for eel farming feed additives that can improve the immune capacity and anti-history of eel and increase the healing effect on trauma that can be caused by dense breeding The purpose is to provide an eel farm food.

상기와 같은 목적을 달성하기 위한 본 발명에 따른 장어양식용 사료는 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 100 중량부에 대해 사료첨가제가 0. 1 내지 0.2 중량부로 첨가되어 있고, 상기 사료 첨가제는 수분함량이 15 내지 20%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램을 혼합한 혼합물에 대해 5 내지 7배 중량의 물에 투입하여 85 내지 90℃에서 저온 열추출한 추출액을 침전시킨 후 여과처리한 여과추출액을 가열하여 300cc로 농축하여 형성된다.Eel culture feed according to the present invention for achieving the above object is 49% by weight crude protein, 3% by weight crude fat, 2% by weight calcium, 2.7% by weight, 3% by weight crude fiber, 18% by weight ash, squid meat The feed additive is added in an amount of 0.01 to 0.2 parts by weight based on 100 parts by weight of the basic feed consisting of 22.3% by weight, and the feed additive is dried mulberry leaves, mulberry branches, and mulberry trees, dried to have a water content of 15 to 20%. 360 grams of mulberry mixed with the same weight ratio of roots, dried to the water content of 15 to 20% 270 grams of the lotus leaf and lotus root cut at the same weight ratio and dried to 15 to 20% moisture content The mixture was mixed with 380 grams of ivy chopped and added to water having a weight of 5 to 7 times to precipitate an extract extracted at low temperature by 85 to 90 ° C., and then the filtrate was filtered and concentrated to 300 cc. .

바람직하게는 상기 사료 첨가제는 상기 혼합물 100 중량부에 대해 수분함량이 15 내지 20%가 되게 건조하여 절단한 토마토 줄기와 잎을 동일 중량비로 혼합한 토마토 11 내지 13 중량부를 더 포함되게 상기 물에 혼합하여 추출한 것을 적용한 다.Preferably, the feed additive is mixed with the water so as to further include 11 to 13 parts by weight of tomatoes mixed with 100 parts by weight of the mixed tomato stem and leaves cut to dry at a water content of 15 to 20% by weight. Apply the extracted data.

또한, 상기 사료 첨가제는 상기 혼합물 100 중량부에 대해 수분함량이 15 내지 20%가 되게 건조하여 절단한 질경이 8 내지 12중량부가 더 포함되는 상기 물에 혼합하여 추출한 것을 적용한다.In addition, the feed additive is applied to the extracted by mixing in the water further comprises 8 to 12 parts by weight of the dried and cut to a water content of 15 to 20% to 100 parts by weight of the mixture.

이상에서 설명한 바와 같이 본 발명에 따른 장어 양식용 사료 첨가제 및 첨가제가 혼합된 사료에 의하면, 양식과정에서 장어의 면역성이 향상되어 폐사율이 현저하게 감소하여 항생제의 사용을 억제할 수 있고, 상처발생 예방 빠른 치유에 의해 외관상 상처흔적이 거의 없으며, 성장효율성도 높일 수 있는 장점을 제공한다.As described above, according to the feed mixed with the additives and additives for eel culture according to the present invention, the immunity of the eel is improved during the aquaculture process, the mortality is significantly reduced, and the use of antibiotics can be suppressed, and the occurrence of wounds is prevented. Fast healing provides almost no scars on the appearance and provides the advantage of increasing growth efficiency.

이하 본 발명에 따른 장어 양식용 사료의 바람직한 실시 예를 상세하게 설명한다.Hereinafter, a preferred embodiment of the eel cultured feed according to the present invention will be described in detail.

본 발명에 따른 장어양식용 사료는 기본 사료와 사료첨가제로 이루어져 있고, 사료첨가제는 뽕으로부터 추출한 뽕추출물 35 내지 37중량퍼센트, 연으로부터 추출한 연추출물 25 내지 27 중량퍼센트, 담쟁이넝쿨로부터 추출한 담쟁이추출물 37 내지 39중량퍼센트로 이루어진다.The feed for eel culture according to the present invention consists of a basic feed and a feed additive, the feed additive is 35 to 37% by weight of the mulberry extract extracted from the mulberry, 25 to 27% by weight of the extract extracted from the lotus, ivy extract 37 extracted from the ivy vine To 39 weight percent.

여기서 뽕추출물은 뽕잎, 뽕나무 가지, 뽕나무 뿌리로부터 추출한 것을 적용한다.The mulberry extract is applied to the extract from the mulberry leaves, mulberry branches, mulberry root.

뽕잎은 수분과 탄수화물, 단백질 및 25종의 아미노산이 들어있으며, 콜레스 테롤의 감소, 지방축적예방 및 감소 기능이 있고, 뽕나무의 가지 및 뿌리는 독성을 제거하는 아스파리긴산과, 알라닌, 출혈성 질환을 예방해주는 루틴, 면역성을 증강시키는 각종 물질이 포함되어 있다.Mulberry leaves contain water, carbohydrates, proteins and 25 amino acids, and reduce cholesterol, prevent and reduce fat, and the branches and roots of mulberry, which remove poisons, alanine and hemorrhagic diseases Routines that prevent and include a variety of substances that enhance immunity.

연추출물은 연뿌리와 연잎으로부터 추출한 것을 적용한다.The lotus extract is extracted from lotus root and lotus leaf.

연은 로메린, 루시페린, 아노다인, 아르메파린 등의 알카로이드, d-n 메티코를라우린, 퀘어세킨, 이소퀴어세틴, 플라보노이드, 주석산이나 구연산등의 천연유기산, 비타민등이 함유되어 있다.The kite contains alkaloids such as romerin, luciferin, anodine, and armeparin, d-n meticolaurin, quercekin, isoquicetin, flavonoids, natural organic acids such as tartaric acid and citric acid, and vitamins.

특히 연잎과 연근에 함유된 로메린(roemerine)과 루시페린(nuciferine)은 진정작용이 있어 밀집사육과정에서 받는 장어의 스트레스를 완화시켜 준다. 또한, 연근에는 식물성 섬유가 풍부하게 들어있고, 이러한 식물성 섬유는 장벽을 적당히 자극하여 장내의 활동을 활발히 해주며 체내의 콜레스테롤 수치를 떨어뜨리는 작용In particular, roemerine and luciferine contained in lotus leaf and lotus root have sedative effects and relieve stress of eel during dense rearing process. In addition, the lotus root is rich in vegetable fiber, which stimulates the barrier moderately to activate the intestinal activity and lower the body's cholesterol level

을 하면, 연근에 함유된 철분과 탄닌성분은 소염 작용이 뛰어나 점막 조직의 염증을 가라앉혀 주므로 장어의 밀집 사육 과정에서 발생되는 외상에 대한 치유력을 높일 수 있다.If you do, iron and tannin components contained in the lotus root is excellent anti-inflammatory action to reduce the inflammation of mucosal tissues can increase the healing power of trauma generated in the dense rearing process of eel.

담쟁이 추출물은 담쟁이 넝쿨로부터 추출한 것을 적용한다.Ivy extract is applied to extract from the ivy vine.

담쟁이덩쿨은 항산화 작용을 하는 레스베라트롤성분이 함유되어 있다.Ivy is an antioxidant that contains resveratrol.

또한, 담쟁이 덩쿨은 줄기에서 단맛이 나며 어혈, 통증을 해소시키는 기능을 한다. 이러한 담쟁이 덩쿨로부터 추출된 담쟁이 추출물은 단맛에 의해 장어의 식욕을 증가시키고, 외상에 대한 치유력도 뛰어남을 확인하였다.In addition, the ivy is sweet in the stem and functions to relieve blood, pain. The ivy extract extracted from these ivy vines increased the appetite of the eel by the sweetness, it was confirmed that the healing power for trauma is also excellent.

담쟁이 덩쿨은 소나무를 타고 자란 것 중 3년 이상이 된 것을 채취한 것을 적용한다.Ivy vines are those that have been harvested on pine for more than three years.

이러한 뽕추출물, 연추출물 및 담쟁이 추출물은 해당 재료를 건조한 후 잘게 썰어서 분말화한 것을 적용하거나, 물 또는 알코올을 이용하여 증류 추출방식에 의해 농축된 추출액을 적용하면 된다.Such mulberry extract, lotus extract and ivy extract may be applied to the powdered by drying the material and finely chopped, or by applying an extract concentrated by distillation extraction method using water or alcohol.

추출액으로 추출하여 적용하는 경우 해당 재료를 물과 혼합하여 85-90℃에 약 4시간 동안 저온 열 추출하고, 열 추출한 1차 추출액을 침전시키 후 여과한 후 다시 1차 추출액을 가열하면서 농축한 것을 적용하면 된다.In case of extracting and applying the extract, the material is mixed with water and extracted by low temperature heat extraction at 85-90 ℃ for about 4 hours, and the precipitated primary extract is precipitated, filtered and concentrated by heating the primary extract again. Apply it.

한편, 뽕추출물, 연추출물 및 담쟁이 추출물로 혼합된 혼합물 100 중량부에 대해 토마토 줄기와 잎으로부터 추출한 토마토 추출물이 11 내지 13 중량부 더 포함되는 것이 바람직하다.On the other hand, with respect to 100 parts by weight of the mixture mixed with mulberry extract, lotus extract and ivy extract, it is preferable to further include 11 to 13 parts by weight of the tomato extract extracted from the tomato stem and leaves.

토마토 줄기와 잎으로부터 추출한 추출물은 양어장의 수질 상에 존재하는 병균 또는 충의 번식을 억제하는 효과가 있음이 확인되었다.Extracts extracted from tomato stems and leaves were found to have an effect of inhibiting the growth of germs or worms present in the water quality of fish farms.

또한, 뽕추출물, 연추출물 및 담쟁이추출물로 혼합된 혼합물 100 중량부에 대해 질경이로부터 추출된 질경이 추출물 8 내지 12중량부가 더 포함된다.In addition, 8 to 12 parts by weight of plantain extract extracted from plantain is further included with respect to 100 parts by weight of the mixture mixed with mulberry extract, lotus extract and ivy extract.

질경이에는 아피게닌, 우루솔산, 프레타기닌, 베타시토스테롤 및 아큐빈등이 함유되어 있다. 특히 질경이에 함유된 성분에는 상처 재생효과가 있어 밀집사육되는 과정에서 발생되는 외상에 대해 빠른 재생이 있음을 확인하였다.Plantain contains apigenin, ursolic acid, pretaginine, beta sitosterol and acubin. In particular, the ingredients contained in plantain has a wound regeneration effect, it was confirmed that there is a rapid regeneration of the trauma generated during the dense breeding process.

이러한 사료첨가제는 앞서 설명된 바와 같이 분말 형태로 시중에 공급되는 장어용 기본사료에 첨가하거나 증류방식에 의해 생성된 추출액을 기본사료에 첨가하면 된다.As described above, the feed additive may be added to the basic feed for eel, which is commercially supplied in powder form, or the extract produced by the distillation method may be added to the basic feed.

바람직하게는 기본사료는 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 것을 적용하는 것이 바람직하다.Preferably, the basic feed is composed of 49% by weight crude protein, 3% by weight crude fat, 2% by weight calcium, 2.7% by weight, 3% by weight crude fiber, 18% by weight ash, 22.3% by weight squid meat. .

이러한 기본 사료에 대해 사료 첨가제를 첨가하는 비율은 분말형태로 적용하는 경우 기본 사료 100중량부에 대해 사료첨가제를 2 내지 5중량부 첨가한다.The ratio of the feed additive to the basic feed is 2 to 5 parts by weight of the feed additive is added to 100 parts by weight of the basic feed when applied in the form of a powder.

또한, 기본 사료에 대해 추출액상의 사료 첨가제로 첨가하는 경우 기본 사료 100중량부에 대해 사료첨가제 추출액 0. 1 내지 0.2 중량부 첨가한다. 사료첨가제 추출액을 기본사료에 첨가하는 방식은 기본사료에 추출액을 혼합하여 반죽한 다음 성형기를 통해 수분이 15%정도 되게 건조된 알갱이 형태로 제조하거나, 알갱이 형태로 형성된 기본사료를 교반하면서 추출액을 스프레이 방식으로 분사하여 침투되게 하는 방식이 적용될 수 있다.In addition, when added as a feed additive in the extract liquid relative to the basic feed is added 0.01 to 0.2 parts by weight of the feed additive extract based on 100 parts by weight of the basic feed. The method of adding the feed additive extract to the basic feed is made by mixing the extract with the basic feed, kneading it, and then preparing the granulated form with 15% moisture by a molding machine, or spraying the extract while stirring the basic feed formed in the granular form. A method of injecting by spraying in a manner may be applied.

이러한 사료첨가제가 첨가된 사료를 장어에 투여하여 사육한 결과 밀집사육과정에서 발생될 수 있는 장어의 상처가 빠르게 치유되고, 면역성의 증가에 의해 폐사율을 억제할 수 있음을 확인하였다.As a result of feeding the feed additives added to the eel and rearing it, it was confirmed that the wound of the eel, which may occur in the dense rearing process, was quickly healed and mortality was suppressed by the increase of immunity.

이하 본 발명을 하기 한 실시 예를 통하여 더욱 상세하게 설명한다. 그러나 이 실시예에 의해 본 발명이 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the present invention is not limited by this embodiment.

<실시예 1><Example 1>

먼저, 수분함량이 15%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램을 6000cc의 물에 투입하여 85 내지 90℃에서 4시간 동안 저온 열추출한 추출액을 침전시킨 후 여과처리한 600cc의 여과추출액을 100℃에서 가열하여 300cc로 농축하여 제1추출액을 제조하였다. 그리고, 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 1000그램에 대해 상기 제1추출액 1.5그램을 스프레이 방식으로 첨가한 제1시료를 제조하였다.First, the dried mulberry leaves, dried mulberry leaves, mulberry branches, mulberry roots in the same weight ratio of 360 grams of mulberry, the moisture content of 15% dried leaves and lotus roots in the same weight ratio 270 grams of mixed lead and 380 grams of ivy, dried and dried to 15% of water, were poured into 6000cc of water and precipitated the extract extracted by low temperature heat extraction for 4 hours at 85-90 ℃. The filtrate was heated at 100 ° C. and concentrated to 300 cc to prepare a first extract. And, the first extract with respect to 1000 grams of the basic feed consisting of 49% by weight of crude protein, 3% by weight of crude fat, 2% by weight of calcium, 2.7% by weight of crude fiber, 3% by weight of crude fiber, 18% by weight of ash, 22.3% by weight of squid meat A first sample to which 1.5 grams was added by spraying was prepared.

다음은 본 출원인이 운영하고 있는 무등민물수산의 생물학적 여과조와 포말 분리기가 설치되어 있는 순환여과식 사육시설을 이용하였으며, 10평 크기로 실험을 위해 구획시켜 준비된 수조에 1년생 실뱀장어 2000수를 투입하고, 상기 제1시료를 급여하면서 100주 동안 양식하여 제1 실험군을 준비하였다. Next, we used a circulating filtration breeding facility equipped with a biological filtration tank and foam separator of Mudeung Freshwater Fisheries operated by the applicant, and put 2000 year-old silk eel into a tank prepared for the experiment with a size of 10 pyeong. And, the first experimental group was prepared by aquaculture for 100 weeks while feeding the first sample.

먹이 급여량은 매 급여시 장어 무게의 2%정도로 공급하였다.Feeding amount was 2% of the weight of eel at each feeding.

<실시예 2><Example 2>

다음은 수분함량이 15%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램 및 수분함량이 15 내지 20%가 되게 건조하여 절단한 토마토 줄기와 잎을 동일 중량비로 혼합한 토마토 120그램을 6600cc의 물에 투입하여 85 내지 90℃에서 4시간 동안 저온 열추출한 추출액을 침전시킨 후 여과처리한 660cc의 여과추출액을 100℃에서 가열하여 335cc로 농축하여 제2추출액을 제조하였다. 그리고, 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중 량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 1000그램에 대해 상기 제2추출액 1.5그램을 스프레이 방식으로 침투되게 첨가한 제2시료를 제조하였다.Next, dried mulberry leaves cut to dry to 15% moisture, mulberry branches, mulberry roots 360 grams mixed with the same weight ratio, and dried leaves cut to 15% moisture content lotus leaf and lotus root at the same weight ratio 270 grams of mixed lead, 380 grams of ivy vine dried and cut to 15% moisture content, and 120 grams of tomatoes mixed with tomato stalks and leaves cut to dry to 15-20% moisture content. 6600cc of water was added to precipitate the extract extracted by low temperature heat extraction for 4 hours at 85 to 90 ° C, and the filtered 660cc filtered extract was heated at 100 ° C and concentrated to 335cc to prepare a second extract. And, the second for a 1000 grams of the basic feed consisting of 49% by weight crude protein, 3% by weight crude fat, 2% by weight calcium, 2.7% by weight phosphorus, 3% by weight crude fiber, 18% by weight ash, 22.3% by weight squid meat A second sample was prepared in which 1.5 grams of the extract was added to penetrate the spray method.

이후, 제2시료를 실험예 1과 동일조건의 또 다른 수조에 투입된 2000마리의 실뱀장어에 급여하면서 100주 동안 양식하여 제2 실험군을 준비하였다. Subsequently, the second sample was fed to another 2000 eels fed into another tank under the same conditions as in Experimental Example 1, and then cultured for 100 weeks to prepare a second experimental group.

<실시예 3><Example 3>

다음은 수분함량이 15%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램 및 수분함량이 15 내지 20%가 되게 건조하여 절단한 토마토 줄기와 잎을 동일 중량비로 혼합한 토마토 120그램 및 수분함량이 15 내지 20%가 되게 건조하여 절단한 질경이 100그램을 7000cc의 물에 투입하여 85 내지 90℃에서 4시간 동안 저온 열추출한 추출액을 침전시킨 후 여과처리한 700cc의 여과추출액을 100℃에서 가열하여 365cc로 농축하여 제3추출액을 제조하였다. 그리고, 조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 1000그램에 대해 상기 제3추출액 1.5그램을 스프레이 방식으로 침투되게 첨가한 제3시료를 제조하였다.Next, dried mulberry leaves cut to dry to 15% moisture, mulberry branches, mulberry roots 360 grams mixed with the same weight ratio, and dried leaves cut to 15% moisture content lotus leaf and lotus root at the same weight ratio 270 grams of mixed lead, 380 grams of ivy vine dried and cut to 15% moisture content and 120 grams of tomatoes mixed with dried tomato stalks and leaves cut to dry at 15-20% moisture content, and 100 grams of the diameter cut and dried to 15 to 20% was added to 7000cc of water to precipitate the extract extracted by low temperature heat extraction for 4 hours at 85 to 90 ° C, and the filtered 700cc filtered extract was filtered at 100 ° C. Heated and concentrated to 365cc to prepare a third extract. And, the third extract with respect to 1000 grams of basic feed consisting of 49% by weight crude protein, 3% by weight crude fat, 2% by weight calcium, 2.7% by weight phosphorus, 3% by weight crude fiber, 18% by weight ash, 22.3% by weight squid meat A third sample was prepared in which 1.5 grams were added to penetrate the spray method.

이후, 제3시료를 실험예 1과 동일조건의 또 다른 수조에 투입된 2000마리의 실뱀장어에 급여하면서 100주 동안 양식하여 제3 실험군을 준비하였다. Thereafter, the third sample was fed to another 2000 eels fed into another tank under the same conditions as in Experimental Example 1, and then cultured for 100 weeks to prepare a third experimental group.

한편, 비교군은 본 발명에 따른 사료첨가제를 사용하지 않은 기본사료만을 이용하여 양식하였다. 이때, 장어에 급여되는 사료의 종류만 달리할 뿐 장어 양식용 수조의 크기, 수온, 산소량, 직사광선 차광 및 사육 마릿수 등 제반조건은 실험군과 비교군이 동일하게 적용하였다.On the other hand, the comparison group was cultured using only the basic feed without using the feed additive according to the present invention. At this time, only the feed type for the eel was different, and the same conditions as the experimental group and the comparative group were applied to the size, water temperature, oxygen amount, direct sunlight shading, and breeding number of eel tanks.

상술한 바와 같이 제1, 2, 3 실험군과 비교군을 양식한 후 장어양식에 따른 육질, 폐사율, 시식한 소비자의 느낌을 실험하였다. As described above, after the first, second, and third experimental groups and the comparative group were cultured, the meat quality, mortality, and taste of consumers who tasted the eel cultures were tested.

(1) 육질분석(1) Quality Analysis

상기 실시예 1, 2, 3에서 얻은 제1, 2, 3실험군과 비교군의 육질을 분석하여 하기 표를 얻었다. 육질분석은 장어를 머리, 내장, 뼈를 제거하고 순수한 근육조직만을 채취하여 분석하였다. 분석방법은 수분분석기, 조단백질은 Kjeldahl질소정량법(N6.25), 조지방은 Soxhlet추출법을 이용하였다.The following table | surface was obtained by analyzing the meat quality of the 1st, 2nd, 3rd experimental group and the comparative group obtained in the said Example 1, 2, 3. In meat analysis, eel was removed from the head, internal organs and bones, and pure muscle tissue was collected and analyzed. The analysis method was water analyzer, crude protein Kjeldahl nitrogen quantitative method (N6.25), crude fat Soxhlet extraction method.

항목Item 제1실험군Experimental group 1 제2실험군2nd experimental group 제3실험군3rd experimental group 비교군Comparison 수분함량Water content 50.9%50.9% 50.6%50.6% 50.8%50.8% 51.1%51.1% 조단백질Crude protein 17.2%17.2% 18.3%18.3% 17.4%17.4% 15.2%15.2% 조지방Crude fat 24.9%24.9% 25.1%25.1% 24.8%24.8% 25.4%25.4%

상기 표 1로부터 알 수 있는 바와 같이 실험군들의 조단백질이 비교군보다 높음을 알 수 있다. As can be seen from Table 1, the crude protein of the experimental groups is higher than that of the comparative group.

(2) 장어사육에 따른 폐사율 (2) Mortality due to eel breeding

폐사율에 대해서는 양식기간 동안 사료를 급여하면서 폐사 여부 및 외상발생여부를 양식일지에 기록하면서 산출하였고, 산출한 결과가 아래의 표 2에 기재되어 있다. The mortality rate was calculated by feeding the feed during the aquaculture period and recording the mortality and incidence of trauma in the aquaculture journal. The results are shown in Table 2 below.

항목Item 제1실험군Experimental group 1 제2실험군2nd experimental group 제3실험군3rd experimental group 비교군Comparison 폐사율Mortality 0.7%0.7% 0.7%0.7% 0.5%0.5% 7.8%7.8%

위 표 2를 통해 알 수 있는 바와 같이 비교군에 대해 실험군들의 폐사율이 급격하게 감소했다.As can be seen from Table 2 above, the mortality rate of the experimental groups was drastically reduced for the comparison group.

또한, 폐사되지는 않았지만, 외관을 관찰한 바에 의하면, 비교군은 잦은 상처발생에 의해 외피에 상처흔적이 많이 분포한 반면, 실험군들은 상처 흔적이 거의 없을 정도로 깨끗하였다.In addition, although not dead, as observed from the appearance, the comparative group was a lot of wound traces on the skin due to frequent wound development, while the experimental group was clean enough to have almost no trace of wound.

또한, 실험군과 비교군에 대해 동일한 수의 개체수 100마리씩을 추출하여 중량을 비교한 결과 비교군에 대해 실험군의 중량이 2.5%정도 높게 나타남을 확인하였다. 이러한 결과는 담쟁이 추출물에 의한 식욕증진 효과로 예측된다.In addition, as a result of comparing the weight by extracting the same number of population of 100 individuals for the experimental group and the comparison group, it was confirmed that the weight of the experimental group was about 2.5% higher for the comparison group. This result is expected to be an appetite boosting effect by the ivy extract.

<3> 시식한 소비자의 느낌조사<3> Surveying taste of consumers

본 실험에 따른 양식 장어 사료를 급여한 장어에 대해 무작위로 착출한 50명에게 비교군의 장어와 같이 시식하게 한 결과 육질이 단단하고, 고소한 맛이 향상되었다고 평가되었다. 또한, 실험군 중 실험군 2 및 실험군 3에 대해서는 비린내가 훨씬 적게 발생한다는 평가를 받았다. 이러한 비린내의 감소는 토마토 추출액에 의한 효과를 예측한다.Fifty randomly harvested eel diets fed the cultured eel diet according to the present experiment were evaluated to have a solid meat and improved taste. In addition, the experimental group 2 and the experimental group 3 of the experimental group was evaluated that much less fishy occurrence. This reduction in fishy predicts the effect by tomato extract.

Claims (3)

조단백질 49중량%, 조지방 3중량%, 칼슘 2중량%, 인 2.7중량%, 조섬유 3중량%, 조회분 18중량%, 오징어육 22.3중량%로 이루어진 기본사료 100 중량부에 대해 사료첨가제가 0. 1 내지 0.2 중량부로 첨가되어 있고,Feed additive is 0. 100 parts by weight of the basic feed consisting of 49% by weight crude protein, 3% crude fat, 2% calcium, 2.7% phosphorus, 3% crude fiber, 18% crude ash, 22.3% squid meat. 1 to 0.2 parts by weight is added, 상기 사료 첨가제는 수분함량이 15 내지 20%가 되게 건조하여 절단한 뽕잎과, 뽕나무 가지, 뽕나무 뿌리를 동일 중량비로 혼합한 뽕 360그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 연잎과 연뿌리를 동일 중량비로 혼합한 연 270그램과, 수분함량이 15 내지 20%가 되게 건조하여 절단한 담쟁이 넝쿨 380그램을 혼합한 혼합물에 대해 5 내지 7배 중량의 물에 투입하여 85 내지 90℃에서 저온 열추출한 추출액을 침전시킨 후 여과처리한 여과추출액을 가열하여 300cc로 농축하여 형성된 것을 적용하는 것을 특징으로 하는 장어양식용 사료.The feed additive is dried mulberry leaves cut to dry to 15 to 20% moisture content, 360 grams of mulberry mixed with mulberry branches, mulberry roots in the same weight ratio, and lotus leaf cut to dry to moisture content 15 to 20% 270 grams of lead and lotus root mixed in the same weight ratio, and 380 grams of dried ivy vine cut to dry with water content of 15 to 20% in water of 5 to 7 times the weight of 85 to 90 ℃ Precipitate eel culture, characterized in that the precipitate formed by extracting the low temperature heat-extracted extract from the filtered filtrate was heated to 300cc concentrated. 삭제delete 삭제delete
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WO2017179856A1 (en) * 2016-04-15 2017-10-19 롯데정밀화학 주식회사 Feed composition for fish farming and dough feed containing same
WO2018169215A1 (en) * 2017-03-15 2018-09-20 롯데정밀화학 주식회사 Feed composition for fish cultivation, feed comprising same, and preparation method therefor
KR102258211B1 (en) 2020-12-04 2021-05-31 어업회사법인 부안수산 Aquatic product farming method using food including mulberry leaves

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JP2005200316A (en) 2004-01-13 2005-07-28 Tooyoo Gijutsu Kenkyusho:Kk Feed, feed additive and chemical for animal comprising pulverized material and/or extracted material of lotus
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KR100585500B1 (en) 2005-01-27 2006-06-07 코미뱀부인터내셔날(주) Feed additives and feed containing thesame for domestic eel(anguilla japonica)
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JP2005200316A (en) 2004-01-13 2005-07-28 Tooyoo Gijutsu Kenkyusho:Kk Feed, feed additive and chemical for animal comprising pulverized material and/or extracted material of lotus
KR20060025015A (en) * 2004-09-15 2006-03-20 박준식 A feed additive containing lotus
KR100585500B1 (en) 2005-01-27 2006-06-07 코미뱀부인터내셔날(주) Feed additives and feed containing thesame for domestic eel(anguilla japonica)
KR20060099192A (en) * 2005-03-10 2006-09-19 (주)바이오뉴트리젠 A composition for feed additive comprising plant powder or extracts containing antioxidant materials

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017179856A1 (en) * 2016-04-15 2017-10-19 롯데정밀화학 주식회사 Feed composition for fish farming and dough feed containing same
WO2018169215A1 (en) * 2017-03-15 2018-09-20 롯데정밀화학 주식회사 Feed composition for fish cultivation, feed comprising same, and preparation method therefor
KR102258211B1 (en) 2020-12-04 2021-05-31 어업회사법인 부안수산 Aquatic product farming method using food including mulberry leaves

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