CN111789199A - Biological agent for optimizing and culturing procambarus clarkii and preparation process thereof - Google Patents

Abstract

The invention discloses a biological preparation for optimizing and culturing procambarus clarkii and a preparation process thereof, which is prepared by mixing, stirring, dehydrating and sterilizing and drying fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract; the fish meal fish oil and soybean oil composite feed comprises, by weight, 20-30 parts of fish meal, 15-25 parts of soybean oligopeptide powder, 40-50 parts of dried vegetables, 5-10 parts of animal and vegetable mixed oil, 2-5 parts of dried fruits and 20-30 parts of fresh plant extract, wherein the animal and vegetable mixed oil is prepared by mixing fish oil and soybean oil in a ratio of 1: 2, in a ratio of 2. According to the biological preparation for optimally breeding the procambarus clarkia and the preparation process thereof, the fresh plant extract is used to ensure that the preparation is rich in bioactive factors, the immunity of the procambarus clarkia after eating is enhanced, the anti-pathological change capability of the procambarus clarkia is improved, and meanwhile, the bioactive factors can effectively purify a water source and improve the ecological environment of the existing procambarus clarkia.

Description

Biological agent for optimizing and culturing procambarus clarkii and preparation process thereof

Technical Field

The invention relates to the technical field of agricultural cultivation, in particular to a biological agent for optimally cultivating procambarus clarkia and a preparation process thereof.

Background

The procambarus clarkia is also called procambarus clarkia, is a freshwater economic shrimp, is popular with people due to delicious meat taste, has the ingestion range including aquatic weeds, algae, aquatic insects, animal carcasses and the like, and is widely cultivated by people due to omnivory, high growth speed and strong adaptability.

The existing procambarus clarkii adopts the traditional conventional feed in the breeding process, the main components of the feed are animal and plant protein powder, trace mineral elements and other food calling additives, however, the existing traditional procambarus clarkii still has the following problems:

1. compared with the natural procambarus clarkii predation environment, the existing traditional feed has simple raw material components and unbalanced nutrition, so that the immunity of the procambarus clarkii is reduced, and the procambarus clarkii is finally damaged;

2. because the animal and plant protein powder is prepared by using animal and plant bodies, protein particles which are not eaten are decomposed in soil, the chemical additive is decomposed along with the feed to pollute underwater soil, the soil activity is reduced, and harmful bacteria are bred to increase the pathological change probability of the procambarus clarkii;

3. once the procambarus clarkii dies due to the pathological change problem, the procambarus clarkii has the self-feeding property, and pathogenic bacteria rapidly spread, so that the procambarus clarkii matrix of the whole farm is diseased, and the economic burden of post-treatment is greatly increased.

Disclosure of Invention

Technical problem to be solved

The invention aims to provide a biological agent for optimizing and culturing procambarus clarkia and a preparation process thereof, which aim to solve the problems in the background technology. The biological preparation for optimizing and culturing the procambarus clarkia and the preparation process thereof provided by the invention have the characteristics of balanced and rich nutrition, optimization of the living environment of the procambarus clarkia and improvement of the immunity of the procambarus clarkia.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a biological preparation for optimally culturing procambarus clarkia is prepared by mixing fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract, stirring, removing water, sterilizing and drying; the fish meal fish oil and soybean oil composite feed comprises, by weight, 20-30 parts of fish meal, 15-25 parts of soybean oligopeptide powder, 40-50 parts of dried vegetables, 5-10 parts of animal and vegetable mixed oil, 2-5 parts of dried fruits and 20-30 parts of fresh plant extract, wherein the animal and vegetable mixed oil is prepared by mixing fish oil and soybean oil in a ratio of 1: 2, in a ratio of 2.

Preferably, the specific preparation process comprises the following steps:

s1, firstly, washing and airing the dried vegetables and the dried fruits by using clean water, respectively soaking the dried vegetables and the dried fruits for five minutes in 10-15% of dilute hydrochloric acid, then taking out the vegetables and the dried fruits, washing the vegetables and the dried fruits by using the clean water, airing the vegetables and the dried fruits, then respectively soaking the vegetables and the dried fruits for three minutes in 10-20% of starch aqueous solution, taking out the vegetables and the dried fruits, quickly washing the vegetables and the dried fruits by using the clean water, and drying the vegetables and the dried fruits for later;

s2, separately crushing the dried vegetables and the dried fruits for later use in S1, and sieving the crushed vegetables and the dried fruits through a sieve of 100 meshes and 120 meshes to respectively prepare vegetable dried powder and fruit dried powder;

s3, introducing the vegetable dry powder and the fruit dry powder in the S2, fish meal, soybean oligopeptide powder, animal and plant mixed oil, fresh plant extract and Chinese herbal medicine powder into a stirrer, mixing and stirring for ten minutes, and guiding out the final mixed liquid substance to be dried for 2 hours at normal temperature for later use;

and S4, sterilizing the mixed liquid finally obtained in the step S3 in a sterile room, and bagging the sterilized mixed liquid in an equal amount.

Preferably, the dried vegetables are any one or two of Chinese cabbage, cauliflower, spinach and green vegetables, and the dried fruits are any one of apples, pineapples, mangoes and bananas.

Preferably, the fresh plant extract is prepared by the following method;

a1, taking 200 parts of a single fresh green prepared leaf, soaking the leaf in 10-15% dilute hydrochloric acid by mass for five minutes, taking out the leaf, washing the leaf with clear water, and airing the leaf;

a2, extracting the leaves in A1 by using a juicer to obtain a first solution, and filtering the first solution through filter paper to obtain a second solution;

a3, adjusting the pH of the second solution in A2 to 6.5-7 to obtain a third solution, hydrolyzing the third solution at 50-60 ℃ for 24 hours, and performing enzyme deactivation treatment to obtain a liquid which is a fresh plant extracting solution.

Preferably, the fresh green prepared leaves are any one of willow leaves, cactus leaves and locust tree leaves.

(III) advantageous effects

The invention provides a biological agent for optimizing and breeding procambarus clarkii and a preparation process thereof, and the biological agent has the following beneficial effects:

(1) according to the biological preparation for optimally breeding the procambarus clarkia and the preparation process thereof, the soybean oligopeptide powder and the dried vegetables are added, so that the organic materials in the breeding preparation are all pure natural materials, are rich in protein, active small peptide and amino acid, provide a high-quality nutrient source which is more beneficial to digestion for the growth of the procambarus clarkia, and ensure the nutrient level of the feed.

(2) According to the biological preparation for optimally breeding the procambarus clarkii and the preparation process thereof, the dried fruits are seasoned, so that the feed is fragrant and pleasant, and the palatability is good, thereby ensuring the feeding rate of the procambarus clarkii, reducing the uneaten proportion of the feed, and reducing the influence of the feed on the soil.

(3) The biological agent for optimally breeding the procambarus clarkii and the preparation process thereof use animal and plant materials completely, so that the double-nutrient of the animal and plant is ensured, and the feed is ensured not to have ecological threat to a breeding area.

(4) According to the biological preparation for optimally breeding the procambarus clarkia and the preparation process thereof, the fresh plant extract is used to ensure that the preparation is rich in bioactive factors, the immunity of the procambarus clarkia after eating is enhanced, the anti-pathological change capability of the procambarus clarkia is improved, and meanwhile, the bioactive factors can effectively purify a water source and improve the ecological environment of the existing procambarus clarkia.

Detailed Description

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The present invention is further described in detail below to enable those skilled in the art to practice the invention with reference to the following description:

embodiment 1, a biological agent for optimizing and culturing procambarus clarkii is prepared by mixing, stirring, dehydrating, sterilizing and drying fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract; the raw materials comprise 20 parts of fish meal, 15 parts of soybean oligopeptide powder, 40 parts of dried vegetables, 5 parts of animal and vegetable mixed oil, 2 parts of dried fruits and 20 parts of fresh plant extract by weight, wherein the animal and vegetable mixed oil is fish oil and soybean oil, and the weight ratio of the fish oil to the soybean oil is 1: 2, in a ratio of 2.

The specific preparation process comprises the following steps:

s1, firstly, washing and airing the dried vegetables and the dried fruits by using clean water, respectively soaking the dried vegetables and the dried fruits for five minutes in 10-15% of dilute hydrochloric acid, then taking out the vegetables and the dried fruits, washing the vegetables and the dried fruits by using the clean water, airing the vegetables and the dried fruits, then respectively soaking the vegetables and the dried fruits for three minutes in 10-20% of starch aqueous solution, taking out the vegetables and the dried fruits, quickly washing the vegetables and the dried fruits by using the clean water, and drying the vegetables and the dried fruits for later;

s2, separately crushing the dried vegetable and the dried fruit for standby in the step S1, and sieving the crushed dried vegetable and the crushed dried fruit with a 100-mesh sieve to respectively prepare dried vegetable powder and dried fruit powder;

s3, introducing the vegetable dry powder and the fruit dry powder in the S2, fish meal, soybean oligopeptide powder, animal and plant mixed oil, fresh plant extract and Chinese herbal medicine powder into a stirrer, mixing and stirring for ten minutes, and guiding out the final mixed liquid substance to be dried for 2 hours at normal temperature for later use;

and S4, sterilizing the mixed solution finally obtained in the step S3 in a sterile room, and bagging the sterilized mixed solution in an equal amount to obtain the dried vegetable, wherein the dried vegetable is one or two of Chinese cabbage, cauliflower, spinach and green vegetable, and the dried fruit is one of apple, pineapple, mango and banana. By adding the soybean oligopeptide powder and the dried vegetables, the organic materials in the culture preparation are all pure natural materials, are rich in protein, active small peptide and amino acid, provide a high-quality nutrient source which is more beneficial to digestion for the growth of the Procambrus clarkii, ensure the nutrient level of the feed, and simultaneously prepare the vegetable powder and the dried fruit powder by the method, ensure the purity of raw material materials, ensure that the powdery particles are conveniently absorbed by the Procambrus clarkii in the screening process, and improve the feeding efficiency.

The fresh plant extract is prepared by the following method;

a1, taking 200 parts of a single fresh green prepared leaf, soaking the leaf in 10-15% dilute hydrochloric acid by mass for five minutes, taking out the leaf, washing the leaf with clear water, and airing the leaf;

a2, extracting the leaves in A1 by using a juicer to obtain a first solution, and filtering the first solution through filter paper to obtain a second solution;

a3, adjusting the pH of the second solution in A2 to 6.5-7 to obtain a third solution, hydrolyzing the third solution at 50-60 ℃ for 24 hours, and performing enzyme deactivation treatment to obtain a liquid which is a fresh plant extract, wherein the fresh plant leaves are any one of willow leaves, cactus leaves and locust tree leaves.

The preparation method has the advantages that the fresh plant extract is used, so that the preparation method is rich in bioactive factors, the immunity of the procambarus clarkia after eating is enhanced, the anti-lesion capacity of the procambarus clarkia is improved, meanwhile, the bioactive factors can effectively purify a water source, and the ecological environment of the existing procambarus clarkia is improved.

Embodiment 2, a biological agent for optimizing and culturing procambarus clarkii is prepared by mixing, stirring, dehydrating, sterilizing and drying fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract; the raw materials comprise 30 parts of fish meal, 25 parts of soybean oligopeptide powder, 50 parts of dried vegetables, 10 parts of animal and vegetable mixed oil, 5 parts of dried fruits and 30 parts of fresh plant extract by weight, wherein the animal and vegetable mixed oil is fish oil and soybean oil, and the weight ratio of the fish oil to the soybean oil is 1: 2, in a ratio of 2.

The specific preparation process comprises the following steps:

s1, firstly, washing and airing the dried vegetables and the dried fruits by using clean water, respectively soaking the dried vegetables and the dried fruits for five minutes in 10-15% of dilute hydrochloric acid, then taking out the vegetables and the dried fruits, washing the vegetables and the dried fruits by using the clean water, airing the vegetables and the dried fruits, then respectively soaking the vegetables and the dried fruits for three minutes in 10-20% of starch aqueous solution, taking out the vegetables and the dried fruits, quickly washing the vegetables and the dried fruits by using the clean water, and drying the vegetables and the dried fruits for later;

s2, respectively crushing the dried vegetable and the dried fruit for standby in the step S1, and sieving the crushed dried vegetable and the crushed dried fruit with a 120-mesh sieve to respectively prepare dried vegetable powder and dried fruit powder;

s3, introducing the vegetable dry powder and the fruit dry powder in the S2, fish meal, soybean oligopeptide powder, animal and plant mixed oil, fresh plant extract and Chinese herbal medicine powder into a stirrer, mixing and stirring for ten minutes, and guiding out the final mixed liquid substance to be dried for 2 hours at normal temperature for later use;

and S4, sterilizing the mixed liquid finally obtained in the step S3 in a sterile room, and bagging the sterilized mixed liquid in an equal amount.

The dried vegetable is one or two of Chinese cabbage, cauliflower, spinach and green vegetable, and the dried fruit is one of apple, pineapple, mango and banana.

The fresh plant extract is prepared by the following method;

a1, taking 200 parts of a single fresh green prepared leaf, soaking the leaf in 10-15% dilute hydrochloric acid by mass for five minutes, taking out the leaf, washing the leaf with clear water, and airing the leaf;

a2, extracting the leaves in A1 by using a juicer to obtain a first solution, and filtering the first solution through filter paper to obtain a second solution;

a3, adjusting the pH of the second solution in A2 to 6.5-7 to obtain a third solution, hydrolyzing the third solution at 50-60 ℃ for 24 hours, and performing enzyme deactivation treatment to obtain a liquid which is a fresh plant extracting solution.

Preferably, the fresh plant leaves are any one of willow leaves, cactus leaves and locust tree leaves.

Embodiment 3, a biological agent for optimizing and culturing procambarus clarkii is prepared by mixing, stirring, dehydrating, sterilizing and drying fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract; the raw materials comprise 25 parts of fish meal, 20 parts of soybean oligopeptide powder, 45 parts of dried vegetables, 7.5 parts of animal and vegetable mixed oil, 3.5 parts of dried fruits and 25 parts of fresh plant extract by weight, wherein the animal and vegetable mixed oil is fish oil and soybean oil, and the weight ratio of the fish oil to the soybean oil is 1: 2, in a ratio of 2.

The specific preparation process comprises the following steps:

s1, firstly, washing and airing the dried vegetables and the dried fruits by using clean water, respectively soaking the dried vegetables and the dried fruits for five minutes in 10-15% of dilute hydrochloric acid, then taking out the vegetables and the dried fruits, washing the vegetables and the dried fruits by using the clean water, airing the vegetables and the dried fruits, then respectively soaking the vegetables and the dried fruits for three minutes in 10-20% of starch aqueous solution, taking out the vegetables and the dried fruits, quickly washing the vegetables and the dried fruits by using the clean water, and drying the vegetables and the dried fruits for later;

s2, separately crushing the dried vegetable and the dried fruit for standby in the step S1, and sieving the crushed dried vegetable and the crushed dried fruit with a 110-mesh sieve to respectively prepare dried vegetable powder and dried fruit powder;

s3, introducing the vegetable dry powder and the fruit dry powder in the S2, fish meal, soybean oligopeptide powder, animal and plant mixed oil, fresh plant extract and Chinese herbal medicine powder into a stirrer, mixing and stirring for ten minutes, and guiding out the final mixed liquid substance to be dried for 2 hours at normal temperature for later use;

and S4, sterilizing the mixed liquid finally obtained in the step S3 in a sterile room, and bagging the sterilized mixed liquid in an equal amount.

The dried vegetable is one or two of Chinese cabbage, cauliflower, spinach and green vegetable, and the dried fruit is one of apple, pineapple, mango and banana.

Preferably, the fresh plant extract is prepared by the following method;

a1, taking 200 parts of a single fresh green prepared leaf, soaking the leaf in 10-15% dilute hydrochloric acid by mass for five minutes, taking out the leaf, washing the leaf with clear water, and airing the leaf;

a2, extracting the leaves in A1 by using a juicer to obtain a first solution, and filtering the first solution through filter paper to obtain a second solution;

a3, adjusting the pH of the second solution in A2 to 6.5-7 to obtain a third solution, hydrolyzing the third solution at 50-60 ℃ for 24 hours, and performing enzyme deactivation treatment to obtain a liquid which is a fresh plant extracting solution.

The fresh plant leaf is one of willow leaf, cactus leaf and locust tree leaf.

Experimental example:

taking four thousand procambarus clarkii in the same period, dividing each 1000 procambarus clarkii into one group, dividing the four groups into four experimental groups, culturing the four experimental groups in the same environment, wherein the conditions of the four experimental groups are the same except that the procambarus clarkii feed is different, the first three groups are fed according to the formula reagent in the embodiment 1-3, the last group is fed by using the traditional feed, and finally, the survival rate data of each group of procambarus clarkii is recorded as follows:

the survival rate of a group of procambarus clarkii is 92.5 percent after three months;

the survival rate of two groups of procambarus clarkii is 95.6 percent after three months;

the survival rate of three groups of procambarus clarkii is 94.1 percent after three months;

the survival rate of four groups of procambarus clarkii is 79.5 percent after three months,

compared with the biological preparation of the procambarus clarkia, the biological preparation of the procambarus clarkia of the invention can obviously improve the weight of the single procambarus clarkia and also can obviously reduce the death rate of the procambarus clarkia.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (5)

1. A biological agent for optimizing and breeding procambarus clarkii is characterized in that: the fish meal-soybean oligopeptide powder is prepared by mixing, stirring, dehydrating, sterilizing and drying fish meal, soybean oligopeptide powder, dried vegetables, animal and plant mixed oil, dried fruits and fresh plant extract; the fish meal fish oil and soybean oil composite feed comprises, by weight, 20-30 parts of fish meal, 15-25 parts of soybean oligopeptide powder, 40-50 parts of dried vegetables, 5-10 parts of animal and vegetable mixed oil, 2-5 parts of dried fruits and 20-30 parts of fresh plant extract, wherein the animal and vegetable mixed oil is prepared by mixing fish oil and soybean oil in a ratio of 1: 2, in a ratio of 2.

2. The biological agent for optimizing the cultivation of procambarus clarkii according to claim 1, wherein: the specific preparation process comprises the following steps:

s1, firstly, washing and airing the dried vegetables and the dried fruits by using clean water, respectively soaking the dried vegetables and the dried fruits for five minutes in 10-15% of dilute hydrochloric acid, then taking out the vegetables and the dried fruits, washing the vegetables and the dried fruits by using the clean water, airing the vegetables and the dried fruits, then respectively soaking the vegetables and the dried fruits for three minutes in 10-20% of starch aqueous solution, taking out the vegetables and the dried fruits, quickly washing the vegetables and the dried fruits by using the clean water, and drying the vegetables and the dried fruits for later;

s2, separately crushing the dried vegetables and the dried fruits for later use in S1, and sieving the crushed vegetables and the dried fruits through a sieve of 100 meshes and 120 meshes to respectively prepare vegetable dried powder and fruit dried powder;

s3, introducing the vegetable dry powder and the fruit dry powder in the S2, fish meal, soybean oligopeptide powder, animal and plant mixed oil, fresh plant extract and Chinese herbal medicine powder into a stirrer, mixing and stirring for ten minutes, and guiding out the final mixed liquid substance to be dried for 2 hours at normal temperature for later use;

and S4, sterilizing the mixed liquid finally obtained in the step S3 in a sterile room, and bagging the sterilized mixed liquid in an equal amount.

3. The process for preparing the biological agent for optimizing the cultivation of the procambarus clarkii according to claim 2, wherein the biological agent comprises the following components: the dried vegetable is one or two of Chinese cabbage, cauliflower, spinach and green vegetable, and the dried fruit is one of apple, pineapple, mango and banana.

4. The biological agent for optimizing the cultivation of procambarus clarkii according to claim 1, wherein: the fresh plant extract is prepared by the following method;

a1, taking 200 parts of a single fresh green prepared leaf, soaking the leaf in 10-15% dilute hydrochloric acid by mass for five minutes, taking out the leaf, washing the leaf with clear water, and airing the leaf;

a2, extracting the leaves in A1 by using a juicer to obtain a first solution, and filtering the first solution through filter paper to obtain a second solution;

a3, adjusting the pH of the second solution in A2 to 6.5-7 to obtain a third solution, hydrolyzing the third solution at 50-60 ℃ for 24 hours, and performing enzyme deactivation treatment to obtain a liquid which is a fresh plant extracting solution.

5. The process for preparing a biological agent for optimizing the cultivation of procambarus clarkii according to claim 4, wherein the biological agent comprises the following components: the fresh green prepared leaf is any one of willow leaf, cactus leaf and locust tree leaf.