CN113558151A - Grouper reinforced feed and preparation method thereof - Google Patents

Abstract

The invention relates to the technical field of aquatic feed production, in particular to a grouper reinforced feed and a preparation method thereof, wherein the grouper reinforced feed is prepared from the following raw materials in parts by weight: shrimp meat: 20-30 parts of a solvent; fish meat: 15-25 parts; crab meat: 15-20 parts of a solvent; sea urchin: 5-10 parts; sea snake tail: 8-12 parts; coral: 10-20 parts; domestic fish meal production: 20-40 parts; meat meal: 15-25 parts; bone meal: 10-18 parts; shrimp meal: 3-10 parts of: chicken intestine powder: 4-15 parts of: spirulina powder: 2-10 parts; chinese herbal medicine antibacterial and antitoxic agents: 15-25 parts; compounding probiotics: 5-10 parts; according to the invention, the Chinese herbal medicine antibacterial and antitoxic agent is adopted, so that the immunity of the grouper can be effectively improved, and the death rate is further reduced; moreover, by adding various seafood meat foods into the reinforced feed, the seafood taste in the feed is enhanced, so that the feed can attract the predation of the groupers better, the intake is improved, and the growth of the groupers is accelerated.

Description

Grouper reinforced feed and preparation method thereof

Technical Field

The invention relates to the technical field of aquatic feed production, in particular to a grouper reinforcing feed and a preparation method thereof.

Background

Grouper belongs to the class of finless fishes, is fat in body and large in mouth, and is not suitable for long-distance rapid swimming. The grouper is quite large in size, the length of the grouper can reach more than one meter, and the condition that the grouper weighs more than one hundred kilograms is not surprising; however, there are many types of groupers and their sizes vary. Inhabit coastal rock reefs, fluctuating sea areas with a lot of gravels, coral reefs, sunken ships, artificial fish reefs and other water areas. The grouper is a typical predatory carnivorous fish, depends on sensitive vision and resolving power of light sensitivity and color sensitivity, often grazes marine organisms such as predatory fish, shrimps, crabs, octopuses, even barnacles and the like fiercely, is particularly favored to eat fresh and live animals, and belongs to endangered rare species.

After the grouper is artificially fed, the activity is greatly reduced, the immunity is obviously reduced, fresh and live marine organisms are not eaten, the growth of the grouper is reduced to different degrees (artificial feed is not interested, the intake of the grouper is not large enough), and the weight of the grouper cannot meet the requirements of farmers, so that the development of the feed capable of improving the immunity of the grouper and enabling the grouper to grow rapidly is needed.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides the grouper strengthening feed and the preparation method thereof, and the immunity of the grouper can be effectively improved by adopting the Chinese herbal medicine antibacterial and antitoxic agent, so that the death rate is reduced; moreover, by adding various seafood meat foods into the reinforced feed, the seafood taste in the feed is enhanced, so that the feed can attract the predation of the groupers better, the intake is improved, and the growth of the groupers is accelerated.

The technical scheme of the invention is as follows:

a grouper reinforced feed and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: shrimp meat: 20-30 parts of a solvent; fish meat: 15-25 parts; crab meat: 15-20 parts of a solvent; sea urchin: 5-10 parts; sea snake tail: 8-12 parts; coral: 10-20 parts; domestic fish meal production: 20-40 parts; meat meal: 15-25 parts; bone meal: 10-18 parts; shrimp meal: 3-10 parts of: chicken intestine powder: 4-15 parts of: spirulina powder: 2-10 parts; chinese herbal medicine antibacterial and antitoxic agents: 15-25 parts; compounding probiotics: 5-10 parts.

Furthermore, the Chinese herbal medicine antibacterial and antitoxic agent is prepared from the following raw materials in parts by weight: codonopsis pilosula: 5-10 parts; ginseng: 10-20 parts of: white atractylodes rhizome: 8-12 parts; ganoderma lucidum: 3-6 parts of: selfheal: 10-20 parts of: buffalo horn: 4-8 parts.

Further, the preparation method of the Chinese herbal medicine antibacterial and antitoxic agent comprises the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation culture medium, inoculating a seed solution of bacillus into the Chinese herbal medicine fermentation culture medium, and fermenting.

Further, the preparation method of the seed liquid of the bacillus comprises the following steps: inoculating Bacillus in seed culture medium, and culturing at 28-32 deg.C for 22-26h (preferably at 30 deg.C for 24 h); the components of the seed medium (liquid form) include peptone, yeast extract and sodium salt.

Further, the preparation method of the Chinese herbal medicine fermentation medium comprises the following steps: mixing the Chinese herbal mixture with testa Tritici, glucose, (NH4)2SO4, KH2PO4 and MgSO4 & 7H2O to obtain culture medium matrix, and mixing the culture medium matrix with water to obtain the final product.

Radix Codonopsis has effects of enhancing stress ability and immunity and resisting bacteria; the ginseng has more than ten trace elements such as potassium, phosphorus, iron, manganese, cobalt, molybdenum, selenium and the like, and the antiviral ability of the ginseng can be effectively improved after the ginseng is absorbed by a body, so that the immunity is improved; the rhizoma atractylodis macrocephalae has the effects of strengthening spleen and tonifying qi, eliminating dampness and promoting diuresis, and relieving abdominal distension and diarrhea, and can enhance the absorption capacity of the organism and improve the intake after the organism absorbs the rhizoma atractylodis macrocephalae; the lucid ganoderma has the functions of improving the immunity of the organism, enhancing the vital signs of the organism and improving the antivirus capability of the organism; the selfheal has the effects of clearing away fire, improving eyesight, eliminating stagnation and reducing swelling, effectively reduces heat toxin in the body, and further improves the immunity of the body; the buffalo horn has the effects of detoxifying, treating typhoid fever and the like.

Further, the composite probiotics comprise clostridium butyricum, lactic acid bacteria, bifidobacteria, lactobacillus acidophilus, actinomycetes and yeasts.

Further, the weight ratio of clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and yeast is 1-3: 0.5-2: 1-2: 0.8-2.4: 1.5-2.5: 0.6-1.4.

Further, the preparation method of the grouper reinforced feed comprises the following steps:

s1, preparing raw materials of each component in parts by weight;

s2, placing the shrimp meat, the fish meat, the crab meat, the sea urchin, the sea snake tail and the coral worm into a sterile stirrer, adding water and stirring until the weight percentage of the water is 36-40%, and stirring to obtain a mixed meat ball;

s3, putting the mixed meat ball, domestic fish meal, meat meal, bone meal, shrimp meal, chicken intestine meal, spirulina powder and Chinese herbal medicine antibacterial and antitoxic agent into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;

s4, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 25-40%, uniformly mixing, adding composite probiotics at 20-30 ℃, continuously stirring until the mixture is mixed, and carrying out sealed anaerobic fermentation culture for 10-20 days to obtain a fermented mixed material;

s5, taking out the fermented mixed material, placing the fermented mixed material in a granulator for granulation, and forming feed granules after granulation;

s6, drying and cooling the prepared feed particles, sieving the feed particles to remove powder, and then storing or packaging the feed particles.

The invention has the beneficial effects that: according to the invention, the Chinese herbal medicine antibacterial and antitoxic agent is adopted, so that the immunity of the grouper can be effectively improved, and the death rate is further reduced; moreover, by adding various seafood meat foods into the reinforced feed, the seafood taste in the feed is enhanced, so that the feed can attract the predation of the groupers better, the intake is improved, and the growth of the groupers is accelerated.

Detailed Description

The following further illustrates embodiments of the invention:

example 1

A grouper reinforced feed and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: shrimp meat: 20 parts of (1); fish meat: 15 parts of (1); crab meat: 15 parts of (1); sea urchin: 5 parts of a mixture; sea snake tail: 8 parts of a mixture; coral: 10 parts of (A); domestic fish meal production: 20 parts of (1); meat meal: 15 parts of (1); bone meal: 10 parts of (A); shrimp meal: 3 parts of: chicken intestine powder: 4 parts of: spirulina powder: 2 parts of (1); chinese herbal medicine antibacterial and antitoxic agents: 15 parts of (1); compounding probiotics: 5 parts of the raw materials.

The Chinese herbal medicine antibacterial and antitoxic agent is prepared from the following raw materials in parts by weight: codonopsis pilosula: 5 parts of a mixture; ginseng: 10 parts of: white atractylodes rhizome: 8 parts of a mixture; ganoderma lucidum: 3 parts of: selfheal: 10 parts of: buffalo horn: 4 parts.

The preparation method of the Chinese herbal medicine antibacterial and antitoxic agent comprises the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation culture medium, inoculating a seed solution of bacillus into the Chinese herbal medicine fermentation culture medium, and fermenting.

The preparation method of the seed liquid of the bacillus comprises the following steps: inoculating bacillus into a seed culture medium, and then culturing for 22h at the temperature of 28 ℃; the components of the seed medium (liquid form) include peptone, yeast extract and sodium salt.

The preparation method of the Chinese herbal medicine fermentation medium comprises the following steps: mixing the Chinese herbal mixture with testa Tritici, glucose, (NH4)2SO4, KH2PO4 and MgSO4 & 7H2O to obtain culture medium matrix, and mixing the culture medium matrix with water to obtain the final product.

The composite probiotics comprise clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and saccharomycetes.

The weight ratio of the clostridium butyricum to the lactobacillus to the bifidobacterium to the lactobacillus acidophilus to the actinomycetes is 1: 0.5: 1: 0.8: 1.5: 0.6.

the preparation method of the grouper reinforced feed comprises the following steps:

s1, preparing raw materials of each component in parts by weight;

s2, placing the shrimp meat, the fish meat, the crab meat, the sea urchin, the sea snake tail and the coral worm into a sterile stirrer, adding water and stirring until the weight percentage of the water is 36%, and stirring to obtain a mixed meat ball;

s3, putting the mixed meat ball, domestic fish meal, meat meal, bone meal, shrimp meal, chicken intestine meal, spirulina powder and Chinese herbal medicine antibacterial and antitoxic agent into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;

s4, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 25%, uniformly mixing, adding composite probiotics at 20 ℃, continuously stirring until the mixture is mixed, and carrying out sealed anaerobic fermentation culture for 10 days to obtain a fermented mixed material;

s5, taking out the fermented mixed material, placing the fermented mixed material in a granulator for granulation, and forming feed granules after granulation;

s6, drying and cooling the prepared feed particles, sieving the feed particles to remove powder, and then storing or packaging the feed particles.

Example 2

A grouper reinforced feed and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: shrimp meat: 30 parts of (1); fish meat: 25 parts of (1); crab meat: 20 parts of (1); sea urchin: 10 parts of (A); sea snake tail: 12 parts of (1); coral: 20 parts of (1); domestic fish meal production: 40 parts of a mixture; meat meal: 25 parts of (1); bone meal: 18 parts of a mixture; shrimp meal: 10 parts of: chicken intestine powder: 15 parts of: spirulina powder: 10 parts of (A); chinese herbal medicine antibacterial and antitoxic agents: 25 parts of (1); compounding probiotics: 10 parts.

The Chinese herbal medicine antibacterial and antitoxic agent is prepared from the following raw materials in parts by weight: codonopsis pilosula: 10 parts of (A); ginseng: 20 parts of: white atractylodes rhizome: 12 parts of (1); ganoderma lucidum: 6 parts of: selfheal: 20 parts of: buffalo horn: 8 parts.

The preparation method of the Chinese herbal medicine antibacterial and antitoxic agent comprises the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation culture medium, inoculating a seed solution of bacillus into the Chinese herbal medicine fermentation culture medium, and fermenting.

The preparation method of the seed liquid of the bacillus comprises the following steps: inoculating bacillus into a seed culture medium, and then culturing for 26h at the temperature of 32 ℃; the components of the seed medium (liquid form) include peptone, yeast extract and sodium salt.

The preparation method of the Chinese herbal medicine fermentation medium comprises the following steps: mixing the Chinese herbal mixture with testa Tritici, glucose, (NH4)2SO4, KH2PO4 and MgSO4 & 7H2O to obtain culture medium matrix, and mixing the culture medium matrix with water to obtain the final product.

The composite probiotics comprise clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and saccharomycetes.

The weight ratio of the clostridium butyricum to the lactobacillus to the bifidobacterium to the lactobacillus acidophilus to the actinomycetes is 3: 2: 2: 2.4: 2.5: 1.4.

the preparation method of the grouper reinforced feed comprises the following steps:

s1, preparing raw materials of each component in parts by weight;

s2, placing the shrimp meat, the fish meat, the crab meat, the sea urchin, the sea snake tail and the coral worm into a sterile stirrer, adding water and stirring until the weight percentage of the water is 40%, and stirring to obtain a mixed meat ball;

s3, putting the mixed meat ball, domestic fish meal, meat meal, bone meal, shrimp meal, chicken intestine meal, spirulina powder and Chinese herbal medicine antibacterial and antitoxic agent into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;

s4, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 40%, uniformly mixing, adding composite probiotics at 30 ℃, continuously stirring until the mixture is mixed, and carrying out sealed anaerobic fermentation culture for 20 days to obtain a fermented mixed material;

s5, taking out the fermented mixed material, placing the fermented mixed material in a granulator for granulation, and forming feed granules after granulation;

s6, drying and cooling the prepared feed particles, sieving the feed particles to remove powder, and then storing or packaging the feed particles.

Example 3

A grouper reinforced feed and a preparation method thereof are disclosed, which comprises the following raw materials in parts by weight: shrimp meat: 25 parts of (1); fish meat: 20 parts of (1); crab meat: 18 parts of a mixture; sea urchin: 7 parts; sea snake tail: 10 parts of (A); coral: 15 parts of (1); domestic fish meal production: 30 parts of (1); meat meal: 20 parts of (1); bone meal: 14 parts of (1); shrimp meal: 6 parts of: chicken intestine powder: 10 parts of: spirulina powder: 6 parts of (1); chinese herbal medicine antibacterial and antitoxic agents: 20 parts of (1); compounding probiotics: 7 parts.

The Chinese herbal medicine antibacterial and antitoxic agent is prepared from the following raw materials in parts by weight: codonopsis pilosula: 8 parts of a mixture; ginseng: 15 parts of: white atractylodes rhizome: 10 parts of (A); ganoderma lucidum: 4 parts of: selfheal: 15 parts of: buffalo horn: 6 parts.

The preparation method of the Chinese herbal medicine antibacterial and antitoxic agent comprises the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation culture medium, inoculating a seed solution of bacillus into the Chinese herbal medicine fermentation culture medium, and fermenting.

The preparation method of the seed liquid of the bacillus comprises the following steps: inoculating bacillus into a seed culture medium, and then culturing for 24 hours at the temperature of 30 ℃; the components of the seed medium (liquid form) include peptone, yeast extract and sodium salt.

The preparation method of the Chinese herbal medicine fermentation medium comprises the following steps: mixing the Chinese herbal mixture with testa Tritici, glucose, (NH4)2SO4, KH2PO4 and MgSO4 & 7H2O to obtain culture medium matrix, and mixing the culture medium matrix with water to obtain the final product.

The composite probiotics comprise clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and saccharomycetes.

Furthermore, the weight ratio of the clostridium butyricum, the lactobacillus, the bifidobacterium, the lactobacillus acidophilus, the actinomycete and the microzyme is 2: 1.2: 1.5: 1.6: 2.0: 1.0.

The preparation method of the grouper reinforced feed comprises the following steps:

s1, preparing raw materials of each component in parts by weight;

s2, placing the shrimp meat, the fish meat, the crab meat, the sea urchin, the sea snake tail and the coral worm into a sterile stirrer, adding water and stirring until the weight percentage of the water is 38%, and stirring to obtain a mixed meat ball;

s3, putting the mixed meat ball, domestic fish meal, meat meal, bone meal, shrimp meal, chicken intestine meal, spirulina powder and Chinese herbal medicine antibacterial and antitoxic agent into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;

s4, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 33%, uniformly mixing, adding composite probiotics at 25 ℃, continuously stirring until the mixture is mixed, and carrying out sealed anaerobic fermentation culture for 15 days to obtain a fermented mixed material;

s5, taking out the fermented mixed material, placing the fermented mixed material in a granulator for granulation, and forming feed granules after granulation;

s6, drying and cooling the prepared feed particles, sieving the feed particles to remove powder, and then storing or packaging the feed particles.

And (3) comparison test:

the commercial feed and the feed obtained in examples 1 to 3 were subjected to comparative tests, which were carried out by the following specific procedures:

in the cage culture process, groupers with strong physique and similar weight are selected for a comparison test, namely the groupers are fed with the feed obtained in the embodiment 1-3, granular feed sold in the groupers is fed for comparison, and the groupers are counted, weighed and compared after being fed for 3 months. The number of the fed animals is 100.

Comparative test data are shown in table 1:

TABLE 1 Effect of commercial and example feeds on the body weight of Epinephelus

Kind of feed	Initial weight (kg)	Ultimate weight (kg)	Weight gain (kg)
				Commercial feed	0.56	0.85	0.29
Example 1	0.54	1.08	0.54
				Example 2	0.55	1.05	0.5
Example 3	0.55	1.15	0.6

The results of the comparative tests show that: the feed obtained in the embodiments 1 to 3 of the invention is adopted to feed the groupers, the weight gain of the groupers is obviously more than that of the groupers fed by the commercial feed, wherein the weight gain of the embodiment 3 is the largest, which shows that the feed obtained in the embodiment 3 has the best effect, so that the reinforced feed obtained by the invention can attract the predation of the groupers better, improve the intake, further accelerate the growth of the groupers, increase the weight and improve the income.

The foregoing embodiments and description have been presented only to illustrate the principles and preferred embodiments of the invention, and various changes and modifications may be made therein without departing from the spirit and scope of the invention as hereinafter claimed.

Claims (8)

1. A grouper reinforced feed and a preparation method thereof are characterized in that: the composition is characterized by comprising the following raw materials in parts by weight: shrimp meat: 20-30 parts of a solvent; fish meat: 15-25 parts; crab meat: 15-20 parts of a solvent; sea urchin: 5-10 parts; sea snake tail: 8-12 parts; coral: 10-20 parts; domestic fish meal production: 20-40 parts; meat meal: 15-25 parts; bone meal: 10-18 parts; shrimp meal: 3-10 parts of: chicken intestine powder: 4-15 parts of: spirulina powder: 2-10 parts; chinese herbal medicine antibacterial and antitoxic agents: 15-25 parts; compounding probiotics: 5-10 parts.

2. The reinforced feed for grouper and the preparation method thereof according to claim 1, wherein the feed comprises: the Chinese herbal medicine antibacterial and antitoxic agent is prepared from the following raw materials in parts by weight: codonopsis pilosula: 5-10 parts; ginseng: 10-20 parts of: white atractylodes rhizome: 8-12 parts; ganoderma lucidum: 3-6 parts of: selfheal: 10-20 parts of: buffalo horn: 4-8 parts.

3. The reinforced feed for grouper and the preparation method thereof according to claim 2, wherein the feed comprises: the preparation method of the Chinese herbal medicine antibacterial and antitoxic agent comprises the following steps: preparing the Chinese herbal medicine mixture into a Chinese herbal medicine fermentation culture medium, inoculating a seed solution of bacillus into the Chinese herbal medicine fermentation culture medium, and fermenting.

4. The reinforced feed for grouper and the preparation method thereof according to claim 3, wherein the feed comprises: the preparation method of the seed liquid of the bacillus comprises the following steps: inoculating bacillus into a seed culture medium, and then culturing for 22-26h at the temperature of 28-32 ℃; the components of the seed culture medium comprise peptone, yeast extract and sodium salt.

5. The reinforced feed for grouper and the preparation method thereof according to claim 3, wherein the feed comprises: the preparation method of the Chinese herbal medicine fermentation medium comprises the following steps: mixing the Chinese herbal mixture with testa Tritici, glucose, (NH4)2SO4, KH2PO4 and MgSO4 & 7H2O to obtain culture medium matrix, and mixing the culture medium matrix with water to obtain the final product.

6. The reinforced feed for grouper and the preparation method thereof according to claim 1, wherein the feed comprises: the composite probiotics comprise clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and saccharomycetes.

7. The reinforced feed for grouper and the preparation method thereof as claimed in claim 6, wherein the feed comprises: the weight ratio of clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes and yeast is 1-3: 0.5-2: 1-2: 0.8-2.4: 1.5-2.5: 0.6-1.4.

8. The reinforced feed for grouper and the preparation method thereof according to claim 1, wherein the feed comprises: the preparation method of the grouper reinforced feed comprises the following steps:

s1, preparing raw materials of each component in parts by weight;

s2, placing the shrimp meat, the fish meat, the crab meat, the sea urchin, the sea snake tail and the coral worm into a sterile stirrer, adding water and stirring until the weight percentage of the water is 36-40%, and stirring to obtain a mixed meat ball;

s3, putting the mixed meat ball, domestic fish meal, meat meal, bone meal, shrimp meal, chicken intestine meal, spirulina powder and Chinese herbal medicine antibacterial and antitoxic agent into a clean and sterile mixer, and fully stirring and mixing to obtain a mixed material;

s4, adding the mixed material into a fermentation tank, adding water until the weight percentage of water is 25-40%, uniformly mixing, adding composite probiotics at 20-30 ℃, continuously stirring until the mixture is mixed, and carrying out sealed anaerobic fermentation culture for 10-20 days to obtain a fermented mixed material;

s5, taking out the fermented mixed material, placing the fermented mixed material in a granulator for granulation, and forming feed granules after granulation;

s6, drying and cooling the prepared feed particles, sieving the feed particles to remove powder, and then storing or packaging the feed particles.