CN105433061A - Microbial fish feed additive and preparation method thereof - Google Patents
Microbial fish feed additive and preparation method thereof Download PDFInfo
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- CN105433061A CN105433061A CN201510988871.3A CN201510988871A CN105433061A CN 105433061 A CN105433061 A CN 105433061A CN 201510988871 A CN201510988871 A CN 201510988871A CN 105433061 A CN105433061 A CN 105433061A
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Abstract
The invention relates to a microbial fish feed additive and a preparation method thereof. The fish feed additive is prepared from the following components in percentage by weight: 25-45 percent of bacillus subtilis, 15-55 percent of lactobacillus, 20-50 percent of streptococcus and 3-15 percent of peony. According to the fish feed additive, the protein source of fish can be increased, growth of harmful bacteria in fish bodies can be inhibited.
Description
Technical field
The present invention is specifically related to a kind of feed addictive, more particularly, relates to a kind of microbiocidal fish feed additive and preparation method thereof.
Background technology
Feed addictive refers at Feed Manufacturing processing, a small amount of or micro substance that adds in use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improve breeding performonce fo animals, ensure animal health, save feed cost, improving the aspects such as livestock products quality has obvious effect.
Aquaculture is the activity in production of breeding, cultivating and gather in the crops aquatic animals and plants under manual control.Generally comprise the overall process forming aquatic products under artificial feeding management from seed.Broadly also Enhancement of fishery resources can be comprised.Aquaculture has the modes such as extensive cultivation, intensive culture and high density culture.Extensive cultivation throws in seed in medium and small natural water area, forms aquatic products completely by natural bait, as lake and reservoir breed fish and shallow sea support shellfish etc.Intensive culture forms aquatic products with bait throwing in, fertilizing method, as aqiuculrue, fish culture in net pen and enclosure culture, net enclosure culture etc. in comparatively small size pond.High density culture adopts flowing water, temperature control, oxygenation and the methods such as high-quality bait of throwing something and feeding, and carries out high-density breeding in small size pond, thus obtains high yield, as Running Water Intensive Fish Farming, shrimp etc.Owing to easily there are some problems in the process of breeding fish, therefore, in fish meal, add some fish feed additives is a kind of conventional means.
Chinese patent CN102715365A discloses a kind of fish feed additive, is made up of the component of following weight portion: flour weevil body powder 1000, food yeast powder 1-2, barrenwort 1-2, insect chitin 4-5, david woodbetony rhizome water extract 1-2.First by yellow meal worm polypide crushed after being dried powdered, then press formula rate and mix with other composition.Fish feed additive of the present invention is a kind of ecological, environmental protective green additive, is added in fish meal, and efficiency of feed utilization improves, and fish resistance against diseases obviously strengthens, and improves the fish speed of growth, improves the survival rate of rainbow trout, improves culture benefit.
Chinese patent CN103404705A discloses a kind of method preparing fish feed additive, it is characterized in that, by weight, by 100 parts water-soluble be more than 90% ammonium humate be dissolved in the water of 200-250 part, filter cleaner obtains ammonium humate solution, then in described ammonium humate solution, add the NaOH of 3-15 part successively, the ferric phosphate of 3-15 part, the calcium phosphate of 3-15 part, the selenium oxide of 3-5 part, total amount is the N of 15-30 part, P and K, obtain fish feed additive mixed liquor, then based on the cumulative volume of this fish feed additive mixed liquor, in this fish feed additive mixed liquor, amino acid is added with the ratio of 100-500g/L, described fish feed additive is obtained after mixing.
Protein and probio are compositions necessary in fish feed, but conventional feed is but difficult to meet this requirement, therefore need a kind of fish feed additive being rich in microorganism badly.
Summary of the invention
In order to solve the problems referred to above that prior art exists, the invention provides a kind of microbiocidal fish feed additive and preparation method thereof, this fish feed additive can increase the protein source of fish and suppress the growth of the harmful bacteria in Fish.
The technical solution adopted in the present invention is:
A kind of microbiocidal fish feed additive, is characterized in that, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 25-45 % by weight, the lactic acid bacteria of 15-55 % by weight, the streptococcus of 20-50 % by weight and 3-15 % by weight.
Preferably, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 30-40 % by weight, the lactic acid bacteria of 20-50 % by weight, the streptococcus of 25-45 % by weight and 5-10 % by weight.
Preferably, described bacillus is be selected from least one in bacillus subtilis, bacillus licheniformis, Bacillus circulans, bacillus cereus and Japan bacillus.
Preferably, described lactic acid bacteria is animal sources lactic acid bacteria and/or Plant Lactobacilli.
Preferably, described streptococcus is be selected from least one in aerobic streptococcus, amphimicrobian streptococcus and streptococcus anaerobius.
Preferably, described fish feed additive also comprises the cassia seed of 3-5 % by weight.
Preferably, described fish feed additive also comprises the saccharomycete of 1-5 % by weight.
A kind of preparation method of microbiocidal fish feed additive, it is characterized in that, after fish feed additive component being mixed according to the formula of fish feed additive of the present invention, at 35-55 DEG C, dry 1-10 hour, then pulverize, obtain described fish feed additive.
Preferably, the temperature of described oven dry is 45-50 DEG C.
Preferably, the time of described oven dry is 2-8 hour.
Beneficial effect of the present invention is: fish feed additive of the present invention can increase the protein source of fish and suppress the growth of the harmful bacteria in Fish, and fish feed additive of the present invention does not have other harmful substance, preparation method is simple, has industrial prospect.
Detailed description of the invention
For making the object of this aspect, technical scheme and advantage clearly, be described in detail to the technical scheme of this aspect below.Obviously, described embodiment is only this aspect part embodiment, instead of whole embodiments.Based on the embodiment in this aspect, other embodiments all that those of ordinary skill in the art obtain under the prerequisite not making creative work, all belong to the scope that this aspect is protected.
The invention provides a kind of microbiocidal fish feed additive, it is characterized in that, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 25-45 % by weight, the lactic acid bacteria of 15-55 % by weight, the streptococcus of 20-50 % by weight and 3-15 % by weight; Preferably, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 30-40 % by weight, the lactic acid bacteria of 20-50 % by weight, the streptococcus of 25-45 % by weight and 5-10 % by weight.
According to the present invention, bacillus (Bacillus) is a section of bacterium, can form bacillus or the coccus of gemma (endospore).Comprise bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and Sporosarcina etc.Injurious factor resistance is strong to external world for they, and distribution is wide, is present in the places such as soil, water, air and animal intestinal.At least one of bacillus of the present invention preferably in bacillus subtilis, bacillus licheniformis, Bacillus circulans, bacillus cereus and Japan bacillus.
According to the present invention, lactic acid bacteria (lacticacidbacteria, LAB) is that a class can utilize fermentable carbohydrate to produce the common name of the bacterium of a large amount of lactic acid.This bacterioid is very extensive in distributed in nature, has abundant species diversity.They are not only research classification, biochemistry, heredity, molecular biology and engineered ideal material, there is important learning value in theory, and also high with human lives's closely-related key areas using value at industry, farming and animal husbandry, food and medicine etc.At least one of streptococcus of the present invention preferably in aerobic streptococcus, amphimicrobian streptococcus and streptococcus anaerobius.
According to the present invention, cassia seed is the dry mature seed of legume Cassia tora or little Cassia tora, have the merit of improving eyesight with it and name it.To gather autumn ripening fruits, dry, lay seed, removing impurity.Cassia seed bitter, sweet, salty, cold nature, enters liver, kidney, large intestine channel; Relax bowel, lipopenicillinase improving eyesight, treatment constipation and high fat of blood, hypertension.Clear liver and improve vision, sharp water defaecation, has laxative action, reducing blood pressure and blood fat.Fish feed additive of the present invention also preferably includes the cassia seed of 3-5 % by weight.
According to the present invention, saccharomycete is unicellular microorganism, belong to the Mycophyta of high microorganism, the same with the cell of higher plant, have nucleus, cell membrane, cell membrane, mitochondria, identical enzyme and metabolisming way, saccharomycete is harmless, easily grows, all there is saccharomycete in air, in soil, in water, in animal body, have oxygen or no oxygen to survive.Fish feed additive of the present invention also preferably includes the saccharomycete of 1-5 % by weight.
The present invention also provides a kind of preparation method of microbiocidal fish feed additive, it is characterized in that, after fish feed additive component being mixed according to the formula of fish feed additive of the present invention, at 35-55 DEG C, preferably at 45-50 DEG C, dry 1-10 hour, preferred oven dry 2-8 hour, then pulverizes, obtains described fish feed additive.
To further illustrate the present invention by embodiment below, but the present invention is not therefore subject to any restriction.
If no special instructions, instrument of the present invention and reagent are and are purchased, and the commodity of different brands do not affect concrete use.
Test case of the present invention and comparative example adopt proportioning to be wheat bran 43%, fish meal 30%, soya-bean cake 15%, barley 10% and fish feed additive 2%, the feed of feed coefficient 2 is as the feed of carp, comparative example does not add fish feed additive, and the ratio of all the other compositions is identical.
Test case of the present invention and comparative example adopt proportioning to be rice bran 43%, soya-bean cake 35%, dried silkworm chrysalis meal 10%, secondary powder 8%, fish feed additive 1%, bone meal 1.50% and salt 0.50%, feed coefficient is that the feed of 2.27 is as the feed of Tilapia mossambica, comparative example does not add fish feed additive, and the ratio of all the other compositions is identical.
Protein increment rate method of testing: get 10 tail fishes before nursing and slaughter rear employing protein testing instrument and measure its protein average content, get 10 tail fishes after nursing to slaughter rear employing protein testing instrument and measure its protein average content, then calculate the increment rate of protein content.
Harmful levels of pathogens growth rate method of testing: get 10 tail fishes before nursing and adopt pathogenic bacteria analyzer to measure salmonella, listeria spp, Listeria Monocytogenes and colibacillary total quantity, getting 10 tail fishes after nursing adopts pathogenic bacteria analyzer to measure salmonella, listeria spp, Listeria Monocytogenes and colibacillary total quantity, then calculates harmful levels of pathogens growth rate.
Embodiment 1
By the bacillus subtilis of 25 % by weight, 55 % by weight milk lactic acid bacteria, 3 % by weight cassia seeds, 17 % by weight aerobic streptococcus and 10 % by weight Chinese herbaceous peony mixing after at 35 DEG C dry 3 hours, then pulverize, obtain described fish feed additive 1.
Embodiment 2
By the bacillus licheniformis of 45 % by weight, the egg lactic acid bacteria of 25 % by weight, the amphimicrobian streptococcus of 20 % by weight and 10 % by weight Chinese herbaceous peony mixing after at 55 DEG C dry 7 hours, then pulverize, obtain described fish feed additive 2.
Embodiment 3
By the Bacillus circulans of 25 % by weight, 25 % by weight soybean lactic acid bacteria, 4 % by weight saccharomycete, 46 % by weight streptococcus anaerobius and 10 % by weight Chinese herbaceous peony mixing after at 35 DEG C dry 10 hours, then pulverize, obtain described fish feed additive 3.
Embodiment 4
By Japan bacillus of 32 % by weight, the potato lactic acid bacteria of 55 % by weight, the streptococcus anaerobius of 20 % by weight and 3 % by weight Chinese herbaceous peony mixing after at 45 DEG C dry 1 hour, then pulverize, obtain described fish feed additive 4.
Test case of the present invention is for illustration of harmful bacteria growth rate in the protein increment rate of fish feed additive of the present invention and body.
Test case 1
Using fish feed additive 2 of the present invention as the feed addictive in carp feed, then drop in pond and feed carp, wherein in pond, the mantissa of carp is 300 tails, body weight is between 100g-1500g, after feeding intake 3 months, the protein increment rate of fish is 180.3%, and harmful levels of pathogens growth rate is-10.34%.
Blank example 1
Any feed addictive will do not added in carp feed, all the other compositions are constant, then drop in pond and feed carp, wherein in pond, the mantissa of carp is 300 tails, body weight is between 100g-1500g, after feeding intake 3 months, the protein increment rate of fish is 98.1%, and harmful levels of pathogens growth rate is 19.38%.
Test case 2
Using fish feed additive 4 of the present invention as the feed addictive in tilapia feed, then drop in pond and feed Luo Fei, wherein in pond, the mantissa of carp is 170 tails, body weight is between 250g-750g, after feeding intake 3 months, the protein increment rate of fish is 201.2%, and harmful levels of pathogens growth rate is-7.11%.
Blank example 1
Any feed addictive will do not added in carp feed, all the other compositions are constant, then drop in pond and feed carp, wherein in pond, the mantissa of carp is 170 tails, body weight between 150g-1500g, after feeding intake 3 months, after feeding intake 3 months, the protein increment rate of fish is 110.9%, and harmful levels of pathogens growth rate is 15.22%.
From embodiment 1-4, fish feed additive preparation method of the present invention is simple, is easy to industrialization.
From test case 1-2, fish feed additive of the present invention significantly can increase the protein source of fish and suppress the growth of the harmful bacteria in Fish.
Claims (10)
1. a microbiocidal fish feed additive, is characterized in that, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 25-45 % by weight, the lactic acid bacteria of 15-55 % by weight, the streptococcus of 20-50 % by weight and 3-15 % by weight.
2. microbiocidal fish feed additive according to claim 1, is characterized in that, this fish feed additive comprises the Chinese herbaceous peony of the bacillus of 30-40 % by weight, the lactic acid bacteria of 20-50 % by weight, the streptococcus of 25-45 % by weight and 5-10 % by weight.
3. microbiocidal fish feed additive according to claim 1, is characterized in that, described bacillus is be selected from least one in bacillus subtilis, bacillus licheniformis, Bacillus circulans, bacillus cereus and Japan bacillus.
4. microbiocidal fish feed additive according to claim 1, is characterized in that, described lactic acid bacteria is animal sources lactic acid bacteria and/or Plant Lactobacilli.
5. microbiocidal fish feed additive according to claim 1, is characterized in that, described streptococcus is be selected from least one in aerobic streptococcus, amphimicrobian streptococcus and streptococcus anaerobius.
6. microbiocidal fish feed additive according to claim 1, is characterized in that, described fish feed additive also comprises the cassia seed of 3-5 % by weight.
7. microbiocidal fish feed additive according to claim 1, is characterized in that, described fish feed additive also comprises the saccharomycete of 1-5 % by weight.
8. the preparation method of a microbiocidal fish feed additive, it is characterized in that, after fish feed additive component being mixed according to the formula of the fish feed additive in claim 1-7 described in any one, at 35-55 DEG C, dry 1-10 hour, then pulverize, obtain described fish feed additive.
9. the preparation method of microbiocidal fish feed additive according to claim 8, is characterized in that, the temperature of described oven dry is 45-50 DEG C.
10. the preparation method of microbiocidal fish feed additive according to claim 8, is characterized in that, the time of described oven dry is 2-8 hour.
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| CN201510988871.3A CN105433061A (en) | 2015-12-23 | 2015-12-23 | Microbial fish feed additive and preparation method thereof |
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| CN201510988871.3A CN105433061A (en) | 2015-12-23 | 2015-12-23 | Microbial fish feed additive and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509337A (en) * | 2016-10-15 | 2017-03-22 | 广西润爽生态农业科技有限公司 | Feed additive capable of preventing and treating diseases in liver and gallbladder of fish and preparation method of feed additive |
| CN107751590A (en) * | 2017-11-08 | 2018-03-06 | 安徽莱姆佳生物科技股份有限公司 | A kind of mutton sheep TRM fermented feed and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106509337A (en) * | 2016-10-15 | 2017-03-22 | 广西润爽生态农业科技有限公司 | Feed additive capable of preventing and treating diseases in liver and gallbladder of fish and preparation method of feed additive |
| CN107751590A (en) * | 2017-11-08 | 2018-03-06 | 安徽莱姆佳生物科技股份有限公司 | A kind of mutton sheep TRM fermented feed and preparation method thereof |
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