CN1973659A - Prawn feed additive - Google Patents

Prawn feed additive Download PDF

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Publication number
CN1973659A
CN1973659A CNA2006101223055A CN200610122305A CN1973659A CN 1973659 A CN1973659 A CN 1973659A CN A2006101223055 A CNA2006101223055 A CN A2006101223055A CN 200610122305 A CN200610122305 A CN 200610122305A CN 1973659 A CN1973659 A CN 1973659A
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CN
China
Prior art keywords
prawn
feed additive
prawn feed
feed
herbal medicine
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CNA2006101223055A
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Chinese (zh)
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CN1973659B (en
Inventor
李卓佳
林黑着
文国樑
陈永青
杨莺莺
于明超
曹煜成
贾晓平
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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South China Sea Fisheries Research Institute Chinese Academy Fishery Sciences
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention discloses one kind of prawn feed additive. The prawn feed additive is compounded with Chinese herbal medicine powder in 8-12 weight portions and solid lichen-forming bacillus material in 12-18 weight portions. The Chinese herbal medicine powder is prepared with isatis root, forthythia, astragalus root, agastache, Viola yedoensis and Chinese goldthread and through stoving and crushing. The solid lichen-forming bacillus material contains lichen-forming bacillus in 10<9> CFU/g. The prawn feed additive is added into prawn feed in the added amount of 0.15-0.50 wt% before the feed is pelletized into 1.5 mm size grains. By means of the synergistic effects of the Chinese medicine materials and the lichen-forming bacillus, the prawn feed additive has the functions of raising the activity of digestive enzyme, promoting the growth of prawn and raising the survival rate of prawn.

Description

The prawn feed additive
Technical field
The present invention relates to a kind of prawn feed additive.
Technical background
Along with the fast development of shrimp culture industry and improving constantly of intensive culture degree, the disease problem is serious day by day.In traditional pest control method, antibiotic and chemicals are used as main means and use in a large number, have caused pathogen to develop immunity to drugs, and result of treatment descends; Simultaneously, because medicine residual, have a strong impact on the health and the Environmental security of consumer groups.Because the aquatic products medicament residue exceeds standard, and causes products export limited, has had a strong impact on the competitiveness of China's aquatic products, has hindered the developing of domestic and international market, has caused enormous economic loss.
Summary of the invention
The object of the present invention is to provide a kind of prawn feed additive, can improve the digestive enzyme activity of prawn, the growth of promotion cultured prawn significantly, reduce the disease problem of prawn culturing.
Prawn feed additive of the present invention is composited by the proportioning of following raw material with parts by weight:
Chinese herbal medicine powder 8-12 bacillus licheniformis solid material 12-18.
Compound Chinese herbal medicine powder of the present invention is composited after being pulverized by the Radix Isatidis that waits weight portion, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and coptis drying.
The bacillus licheniformis flora is 10 in the bacillus licheniformis complex solid material of the present invention 9CFU/g.
The percentage by weight that adds in prawn feed of compound additive of the present invention is 0.15%-0.50%, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
Beneficial effect of the present invention: the present invention adds an amount of Chinese herbal medicine and bacillus licheniformis in prawn feed, by their synergy, can obviously improve digestive enzyme activity, promote the growth of prawn and improve the prawn survival rate, have good economic benefit and social benefit, help the sustainable sound development of prawn culturing, meet the development strategy trend of aquatic feeds.
The specific embodiment
Radix Isatidis, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and the coptis of weight portion such as get, concrete preparation process is: bulk drug → first drying → secondary drying → coarse crushing → ultramicro grinding → sieve → composite, make compound Chinese herbal medicine powder.
Get culture medium (composition: cornstarch 2%, peptone 2.5%, corn steep liquor 1.5%, NaH2PO40.8%; PH7.2-7.4) → sterilize (121 ℃, 30min) → cooling (30 ℃) → (30 ℃ of (inoculum concentration 5%) → seed tank culture of inoculation, 18h) → enlarge and cultivate (30 ℃, 24h) → centrifugal → be written into carrier (wheat bran) → drying → pulverizing, make bacillus licheniformis complex solid material, the bacillus licheniformis flora is 10 in the above-mentioned bacillus licheniformis complex solid material 9CFU/g.
Take by weighing the above-mentioned raw material that makes, mix, make the prawn feed additive, wherein Chinese herbal medicine powder 8-12 with the proportioning of parts by weight; Bacillus licheniformis solid material 12-18.
Above-mentioned compound additive directly adds in the prawn feed, and addition is the 0.15%-0.50% of prawn feed weight, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
The experiment of feeding prawn with the present invention is as follows:
Experimental result adopts average ± standard deviation to represent, uses one-way analysis of variance (ANOVA) statistics, when having significant difference between the ANOVA demonstration is handled, uses Duncan ' s multiple ratio with the analysis bank differences.SPSS 11.5 statistical softwares are used in all statistical analyses, are the significance of difference with P＜0.05.
Prepare five kinds of prawn feeds, feed A: prawn basal feed; Feed B: in basal feed, add 0.2% above-mentioned compound Chinese herbal medicine powder; Feed C: bacillus licheniformis feed group, in basal feed, add 0.30% above-mentioned bacillus licheniformis; Feed D: in basal feed, add 0.25% Chinese herbal medicine of the present invention and bacillus licheniformis compound additive; Feed E: in basal feed, add 0.50% Chinese herbal medicine of the present invention and bacillus licheniformis compound additive.The particle that particle diameter is 1.5mm is made in granulation behind the mixing.
With above-mentioned prawn mixed feed, fed initial body weight 2.03g Environment of Litopenaeus vannamei Low (Litopenaeus vannamei) 70 days.In indoor cement circle pond 2.0m 3Grouping is carried out.Culturing water filters through post precipitation.Shrimp 80 tails are supported in every pond, and every group of 3 in feed is parallel.Keep continuous charge.The breeding process water temperature is 19.6-25.2 ℃, salinity 30～31 ‰.
Culture efficiency sees Table 1.(feed A) compares with control group, in the feed separately or associating wherein add bacillus licheniformis and Chinese herbal medicine, can both promote the growth of prawn.Especially, feed D group is significantly higher than other each group, does not compare with the Chinese herbal medicine group with adding bacillus licheniformis, and rate of body weight gain improves 35%.
The throw something and feed Environment of Litopenaeus vannamei Low culture efficiency of DIFFERENT FEED of table 1
Group A B C D E
Survival rate (%) rate of body weight gain (%) 95.83±1.44 116.89±3.45 a 97.92±1.91 136.17±5.02 b 97.50±1.25 127.89±3.55 ab 98.33±0.73 161.65±17.68 c 96.67±1.91 137.08±11.66 b
(average ± SD) there is significant difference (p＜0.05) in expression to same line data between the different persons of subscript letter
With above-mentioned prawn mixed feed, fed initial body weight 1.9g Environment of Litopenaeus vannamei Low (Litopenaeus vannamei) 56 days.In indoor cement pit (1.0 * 1.0 * 1.0m 3) divide into groups to carry out, culture water and filter through post precipitation.Shrimp 100 tails are supported in every pond, and every group of 3 in feed is parallel.Keep continuous charge.The breeding process water temperature is 19.6-25.2 ℃, salinity 30～31 ‰.Experimental session was taken a sample once every 2 weeks, and shrimp 10 tails are got in each every pond, and the liver pancreas of taking-up is put in-70 ℃ of ultra low temperature freezers and preserves until the digestive ferment analysis.
The mensuration of prolease activity is substrate with reference to Anson (1938) with the casein, and Folin reagent is a developer; The mensuration of amylase activity is with reference to Bernfeld (1955), with soluble starch as substrate, 3,5-dinitrosalicylic acid developer cessation reaction; The mensuration of the white concentration of enzyme liquid eggs is made standard with bovine serum albumin, measures (Bradford, 1976) with the Coomassie brilliant blue method.
The proteinase activity of liver and amylase activity analysis see Table 2 and table 3 respectively.In all 4 sub-samplings, the independent and common interpolation of bacillus licheniformis and Chinese herbal medicine has all improved the protease and the amylase activity of Environment of Litopenaeus vannamei Low liver pancreas.Especially, the 2nd week, the protease and the amylase activity of the additive group of the present invention (feed D) of interpolation 0.25% all were higher than control group (feed A) more than 60% when taking a sample, and the result of 4 sub-samplings on average improves 30%.
The throw something and feed variation of Environment of Litopenaeus vannamei Low liver proteinase activity (IU/mg albumen) of DIFFERENT FEED of table 2
Group A B C D E
6 all 8 weeks of 4 weeks in 2 weeks 0.082±0.005 a 0.102±0.004 0.142±0.006 a 0.096±0.019 0.102±0.010 ab 0.116±0.016 0.142±0.009 a 0.103±0.007 0.100±0.010 ab 0.110±0.009 0.155±0.007 ab 0.127±0.026 0.132±0.030 b 0.118±0.015 0.166±0.007 b 0.116±0.009 0.114±0.017 ab 0.116±0.019 0.151±0.008 ab 0.105±0.007
(average ± SD) there is significant difference (p＜0.05) in expression to same line data between the different persons of subscript letter
The throw something and feed variation of Environment of Litopenaeus vannamei Low liver amylase activity (IU/mg albumen) of DIFFERENT FEED of table 3
Group A B C D E
6 all 8 weeks of 4 weeks in 2 weeks 0.671±0.094 a 0.864±0.175 1.130±0.022 a 0.914±0.111 a 0.787±0.123 a 0.925±0.116 1.242±0.113 abc 0.913±0.022 a 0.883±0.104 ab 0.986±0.080 1.337±0.054 bc 1.192±0.154 b 1.131±0.260 b 0.974±0.144 1.377±0.021 c 0.973±0.062 a 0.876±0.139 ab 0.978±0.091 1.176±0.143 ab 0.942±0.050 a
(average ± SD) there is significant difference (p＜0.05) in expression to same line data between the different persons of subscript letter

Claims (4)

1, a kind of prawn feed additive is characterized in that being composited by the proportioning of following raw material with parts by weight:
Chinese herbal medicine powder 8-12 bacillus licheniformis solid material 12-18.
2, prawn feed additive according to claim 1 is characterized in that being composited after described compound Chinese herbal medicine powder is pulverized by the Radix Isatidis that waits weight portion, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and coptis drying.
3, prawn feed additive according to claim 1 is characterized in that the bacillus licheniformis flora is 10 in the described bacillus licheniformis complex solid material 9CFU/g.
4, a kind of application rights requires the method for 1 additive, it is characterized in that the interpolation percentage by weight in prawn feed is 0.15%-0.50%, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
CN2006101223055A 2006-09-22 2006-09-22 Prawn feed additive Active CN1973659B (en)

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CN2006101223055A CN1973659B (en) 2006-09-22 2006-09-22 Prawn feed additive

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Application Number Priority Date Filing Date Title
CN2006101223055A CN1973659B (en) 2006-09-22 2006-09-22 Prawn feed additive

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CN1973659B CN1973659B (en) 2010-07-21

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101438772B (en) * 2008-12-26 2011-06-08 集美大学 Compound protein feed for shrimp and method for preparing the same
CN103518996A (en) * 2013-09-03 2014-01-22 孙文秀 Shrimp feed additive
CN105249022A (en) * 2015-11-11 2016-01-20 浙江锦天生物科技有限公司 Compound micro-ecological feed additives for litopenaeus vannamei
CN105360715A (en) * 2015-11-09 2016-03-02 杭州亿尔生物科技有限公司 Traditional Chinese medicine microbial composite additive for adjusting shrimp body color
CN105433061A (en) * 2015-12-23 2016-03-30 广州聚注专利研发有限公司 Microbial fish feed additive and preparation method thereof
CN113046275A (en) * 2021-04-27 2021-06-29 中国海洋大学 Bacillus used for prawn feed and fermented Chinese herbal medicine preparation used for prawn feed

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1620892A (en) * 2004-12-10 2005-06-01 中国海洋大学 Compound microecological preparation for prawn

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101438772B (en) * 2008-12-26 2011-06-08 集美大学 Compound protein feed for shrimp and method for preparing the same
CN103518996A (en) * 2013-09-03 2014-01-22 孙文秀 Shrimp feed additive
CN105360715A (en) * 2015-11-09 2016-03-02 杭州亿尔生物科技有限公司 Traditional Chinese medicine microbial composite additive for adjusting shrimp body color
CN105249022A (en) * 2015-11-11 2016-01-20 浙江锦天生物科技有限公司 Compound micro-ecological feed additives for litopenaeus vannamei
CN105433061A (en) * 2015-12-23 2016-03-30 广州聚注专利研发有限公司 Microbial fish feed additive and preparation method thereof
CN113046275A (en) * 2021-04-27 2021-06-29 中国海洋大学 Bacillus used for prawn feed and fermented Chinese herbal medicine preparation used for prawn feed

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