CN111296673A - Laying duck fermented feed and application thereof - Google Patents
Laying duck fermented feed and application thereof Download PDFInfo
- Publication number
- CN111296673A CN111296673A CN202010147726.3A CN202010147726A CN111296673A CN 111296673 A CN111296673 A CN 111296673A CN 202010147726 A CN202010147726 A CN 202010147726A CN 111296673 A CN111296673 A CN 111296673A
- Authority
- CN
- China
- Prior art keywords
- culture
- fermented feed
- laying
- fermentation
- bacillus subtilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000272525 Anas platyrhynchos Species 0.000 title claims abstract description 40
- 238000000855 fermentation Methods 0.000 claims abstract description 43
- 241000193171 Clostridium butyricum Species 0.000 claims abstract description 39
- 230000004151 fermentation Effects 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 35
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 32
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 30
- 239000006041 probiotic Substances 0.000 claims abstract description 25
- 235000018291 probiotics Nutrition 0.000 claims abstract description 25
- 230000000529 probiotic effect Effects 0.000 claims abstract description 21
- 239000002994 raw material Substances 0.000 claims abstract description 17
- 230000001965 increasing effect Effects 0.000 claims abstract description 9
- KJPRLNWUNMBNBZ-QPJJXVBHSA-N (E)-cinnamaldehyde Chemical compound O=C\C=C\C1=CC=CC=C1 KJPRLNWUNMBNBZ-QPJJXVBHSA-N 0.000 claims abstract description 8
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 8
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims abstract description 8
- 235000003434 Sesamum indicum Nutrition 0.000 claims abstract description 8
- 240000008042 Zea mays Species 0.000 claims abstract description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 8
- 235000005822 corn Nutrition 0.000 claims abstract description 8
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 claims abstract description 8
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 claims abstract description 8
- 235000012141 vanillin Nutrition 0.000 claims abstract description 8
- 229940117916 cinnamic aldehyde Drugs 0.000 claims abstract description 5
- KJPRLNWUNMBNBZ-UHFFFAOYSA-N cinnamic aldehyde Natural products O=CC=CC1=CC=CC=C1 KJPRLNWUNMBNBZ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000001079 digestive effect Effects 0.000 claims abstract description 4
- 241000272517 Anseriformes Species 0.000 claims abstract 10
- 244000000231 Sesamum indicum Species 0.000 claims abstract 3
- 239000001963 growth medium Substances 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 20
- 238000012258 culturing Methods 0.000 claims description 19
- 238000009423 ventilation Methods 0.000 claims description 14
- 239000002054 inoculum Substances 0.000 claims description 11
- 230000003321 amplification Effects 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 230000036039 immunity Effects 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 7
- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 230000003213 activating effect Effects 0.000 claims description 4
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000005469 granulation Methods 0.000 claims description 4
- 230000003179 granulation Effects 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 238000010563 solid-state fermentation Methods 0.000 claims description 4
- 238000005507 spraying Methods 0.000 claims description 4
- 230000007480 spreading Effects 0.000 claims description 4
- 238000003892 spreading Methods 0.000 claims description 4
- 230000002708 enhancing effect Effects 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 11
- 235000019750 Crude protein Nutrition 0.000 abstract description 7
- 150000007524 organic acids Chemical class 0.000 abstract description 7
- 229920001503 Glucan Polymers 0.000 abstract description 6
- 229920000057 Mannan Polymers 0.000 abstract description 6
- 244000144972 livestock Species 0.000 abstract description 4
- 235000019156 vitamin B Nutrition 0.000 abstract description 4
- 239000011720 vitamin B Substances 0.000 abstract description 4
- 235000019764 Soybean Meal Nutrition 0.000 abstract 1
- 235000012054 meals Nutrition 0.000 abstract 1
- 239000004455 soybean meal Substances 0.000 abstract 1
- 241000272522 Anas Species 0.000 description 15
- 239000000463 material Substances 0.000 description 12
- 239000000047 product Substances 0.000 description 10
- 230000017448 oviposition Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 238000001514 detection method Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000011265 semifinished product Substances 0.000 description 6
- 241000207961 Sesamum Species 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 238000004321 preservation Methods 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- 241001465754 Metazoa Species 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 230000029087 digestion Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000004806 packaging method and process Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 239000006030 antibiotic growth promoter Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- -1 butyric acid Chemical class 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/111—Aromatic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/25—Shaping or working-up of animal feeding-stuffs by extrusion
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
A laying duck fermented feed and application thereof belong to the technical field of livestock feed. The laying duck fermented feed is obtained by adding a newly prepared probiotic liquid fermentation raw material and then granulating, wherein the probiotic liquid comprises candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 in a volume ratio of 5:4: 1. The raw materials comprise 10-30% of soybean meal, 2-5% of sesame meal, 12-35% of corn, 10-20% of bran, 5-15% of yellow wine lees, 5-20% of cassava dregs, 0.5-2% of cinnamaldehyde and 0.1-0.5% of vanillin. The product of the invention contains more than 16 percent of crude protein, more than 3.7 percent of glucan and mannan, more than 3 percent of organic acid and more than 250mg/kg of B vitamins. By adding 3-6% of the product, the digestive ability of the laying ducks can be improved, the laying rate is improved by more than 5%, and the weight of eggs is increased by more than 3 g.
Description
Technical Field
The invention belongs to the technical field of livestock feed, and particularly relates to a fermented feed for laying ducks and application thereof.
Background
Because the modern scale breeding adopts high-density breeding, hardware facilities fall behind, the environmental control capability is low, and animals are in a sub-clinical state for a long time, so that the immunity and disease resistance are reduced, and a series of diseases are further caused. In order to control diseases and reduce death loss, a large amount of medicaments are added in people consciously or unconsciously, so that the animal immunity is damaged, the environment is seriously damaged, and the serious threat to the human health is formed.
The prohibition of antibiotics is a trend, and the use of antibiotics as growth promoters in the livestock breeding industry is formally prohibited by the ministry of agricultural rural areas from 7 months to 1 day in 2020. The fermented feed is a potential substitute of antibiotics, and is a research hotspot in the field of animal nutrition. The fermented feed is prepared by performing in-vitro fermentation pretreatment on feed raw materials by using single probiotics or combined probiotics, can generate a large amount of probiotic metabolic products, and has the functions of improving the feed utilization rate, improving the animal health, reducing ammonia gas in livestock and poultry houses and the like.
Therefore, in the laying duck production process, the conditions of improving the immunity and the laying rate of the laying ducks, reducing the occurrence of diseases and the like are especially important for solving the problems of duck respiratory diseases, enteritis, hose conveying inflammation and the like through the regulation and control of the feed, and further realizing the efficient duck breeding.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a laying duck fermented feed and application thereof. The invention adopts candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 for synergistic fermentation, the crude protein content in the product is more than 16%, glucan and mannan are more than 3.7%, organic acid is more than 3%, and the content of B vitamins is more than 250 mg/kg. 3 to 6 percent of the product is added in the process of breeding and feeding the laying ducks, so that the immunity of the laying ducks can be increased, the digestion capacity of the laying ducks is improved, the laying rate is improved by more than 5 percent, and the weight of eggs is increased by more than 3 g/egg.
In order to realize the purpose, the following technical scheme is adopted:
the laying duck fermented feed is characterized by being prepared through the following steps:
(1) uniformly mixing 10-30% of bean pulp, 2-5% of sesame pulp, 12-35% of corn, 10-20% of bran, 5-15% of yellow wine lees, 5-20% of cassava dregs, 0.5-2% of cinnamaldehyde and 0.1-0.5% of vanillin, roughly crushing to 40 meshes, completely passing through the coarse grains, sterilizing and curing by saturated steam for 10min, and cooling to 30 ℃;
(2) respectively activating Candida utilis KF-1, Bacillus subtilis strain KF-2 and Clostridium butyricum CICC23847, preparing shake flask seeds, inoculating to culture medium, respectively expanding and culturing to obtain strain liquid with concentration of 2 × 1010After cfu/ml is finished, mixing candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 according to the volume ratio of 5:4:1 to obtain probiotic liquid;
(3) spraying the probiotic liquid obtained in the step (2) to the raw material obtained in the step (1) through a full-automatic nozzle, and fully mixing, wherein the addition amount of the probiotic liquid is 10% -32%;
(4) and (3) performing fermentation treatment, drying by airflow after the fermentation is finished, performing superfine grinding to break the wall, sieving by a 80-mesh sieve, and performing granulation and extrusion to obtain the laying duck fermented feed.
The laying duck fermented feed is characterized in that the expanded culture conditions of the candida utilis KF-1 in the step (2) are as follows: culturing with yeast culture medium at an inoculum size of 10%, culturing temperature of 30 deg.C for 36-72h, stirring speed of 100r/min, and ventilation ratio of 1: 1; the amplification culture conditions of the bacillus subtilis strain KF-2 are as follows: culturing with LB medium with inoculum size of 10%, culturing temperature of 35-40 deg.C, culturing time of 24-48h, stirring speed of 100r/min, and ventilation ratio of 1:1
The laying duck fermented feed is characterized in that the expanded culture conditions of clostridium butyricum CICC23847 in the step (2) are as follows: standing in anaerobic fermenter, and culturing in RCM culture medium for 48-72 hr.
The egg-laying duck fermented feed is characterized in that the fermentation treatment conditions in the step (3) are as follows: anaerobic solid state fermentation is adopted, the spreading thickness is 50-150cm, the fermentation time is 48-72h, and the fermentation is carried out once when the temperature reaches 50 ℃.
A preparation method of a laying duck fermented feed is characterized by comprising the following steps:
(1) uniformly mixing 10-30% of bean pulp, 2-5% of sesame pulp, 12-35% of corn, 10-20% of bran, 5-15% of yellow wine lees, 5-20% of cassava dregs, 0.5-2% of cinnamaldehyde and 0.1-0.5% of vanillin, roughly crushing to 40 meshes, completely passing through the coarse grains, sterilizing and curing by saturated steam for 10min, and cooling to 30 ℃;
(2) respectively activating Candida utilis KF-1, Bacillus subtilis strain KF-2 and Clostridium butyricum CICC23847, preparing shake flask seeds, inoculating to culture medium, respectively expanding and culturing to obtain strain liquid with concentration of 2 × 1010After cfu/ml is finished, mixing candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 according to the volume ratio of 5:4:1 to obtain probiotic liquid;
(3) spraying the probiotic liquid obtained in the step (2) to the raw material obtained in the step (1) through a full-automatic nozzle, and fully mixing, wherein the addition amount of the probiotic liquid is 10% -32%;
(4) and (3) performing fermentation treatment, drying by airflow after the fermentation is finished, performing superfine grinding to break the wall, sieving by a 80-mesh sieve, and performing granulation and extrusion to obtain the laying duck fermented feed.
The laying duck fermented feed is characterized in that the expanded culture conditions of the candida utilis KF-1 in the step (2) are as follows: the yeast culture medium is adopted for sample culture, the inoculation amount is 10%, the culture temperature is 30 ℃, the culture time is 36-72h, the rotating speed of a stirrer is 100r/min, and the ventilation ratio is 1: 1.
The egg-laying duck fermented feed is characterized in that the amplification culture conditions of the bacillus subtilis strain KF-2 in the step (2) are as follows: adopting LB culture medium for culture, the inoculum size is 10%, the culture temperature is 35-40 ℃, the culture time is 24-48h, the rotating speed of a stirrer is 100r/min, and the ventilation ratio is 1: 1.
The laying duck fermented feed is characterized in that the expanded culture conditions of clostridium butyricum CICC23847 in the step (2) are as follows: standing in anaerobic fermenter, and culturing in RCM culture medium for 48-72 hr.
The egg-laying duck fermented feed is characterized in that the fermentation treatment conditions in the step (3) are as follows: anaerobic solid state fermentation is adopted, the spreading thickness is 50-150cm, the fermentation time is 48-72h, and the fermentation is carried out once when the temperature reaches 50 ℃.
The laying duck fermented feed is applied to enhancing the digestive performance of the laying duck, improving the immunity of the laying duck, improving the laying rate and increasing the egg weight.
The Candida utilis KF-1 (with the preservation number of CGMCC No. 7691), the Bacillus subtilis KF-2 (with the preservation number of CGMCC No. 7690) and the Clostridium butyricum (with the preservation number of CICC 23847) are all the existing strains.
The invention has the following beneficial effects:
the invention adopts the candida utilis, the bacillus subtilis and the clostridium butyricum to carry out synergistic fermentation, so that the fermentation efficiency can be obviously improved, the contents of protein and small peptide (amino acid) and B vitamins in the product can be improved, the contents of organic acid, particularly butyric acid, can be improved, and the use of antibiotics in the feed for laying ducks can be effectively reduced. The product has crude protein content of more than 16%, glucan and mannan of more than 3.7%, organic acid of more than 3%, and vitamin B content of more than 250 mg/kg. 3 to 6 percent of the product is added in the process of breeding and feeding the laying ducks, so that the immunity of the laying ducks can be increased, the digestion capacity of the laying ducks is improved, the laying rate is improved by more than 5 percent, and the weight of eggs is increased by more than 3 g/egg.
Detailed Description
In order to make the object and technical solution of the present invention more apparent, the present invention is described in further detail below. The Candida utilis (Candida utilis) KF-1 in the composite zymophyte has a preservation number of CGMCC No. 7691; bacillus subtilis strain KF-2 with preservation number of CGMCC No. 7690; clostridium butyricum (Clostridium butyricum) is purchased from China center for Industrial culture Collection of microorganisms with the collection number CICC 23847.
Example 1:
(1) preparing raw materials: uniformly mixing 20% of bean pulp, 5% of sesame pulp, 25% of corn, 15% of bran, 15% of yellow wine lees, 19% of cassava residue, 0.9% of cinnamyl aldehyde and 0.1% of vanillin according to a weight ratio, coarsely crushing the mixture to 40 meshes by using a crusher, conveying the mixture to a conditioner, introducing saturated steam for sterilization and curing, wherein the time in the conditioner is 10min, and cooling to 30 ℃.
(2) Preparing a composite fermentation liquid: candida utilis (A), (B) and (C)Candida utilis) Strain KF-1, Bacillus subtilis (B.subtilis)Bacillus subtilis) Strain KF-2, Clostridium butyricum (C.butyricum) ((C.butyricum))Clostridium butyricum) CICC23847 is respectively activated in a laboratory, and shake flask seeds are manufactured; respectively performing amplification culture, culturing Candida utilis KF-1 in yeast culture medium with inoculum concentration of 10%, culturing temperature of 30 deg.C, stirring at 100r/min and ventilation ratio of 1:1 for 36-72 hr until the concentration of bacteria liquid reaches 2 × 109cfu/ml; bacillus subtilis (A), (B) and (C)Bacillus subtilis) The strain KF-2 is subjected to amplification culture with LB culture medium, the inoculum size is 10%, the culture temperature is 35-40 deg.C, the rotation speed of stirrer is 100r/min, the ventilation ratio is 1:1, and the culture time is 24-48h to 2 × 1010cfu/ml; clostridium butyricum (C)Clostridium butyricum) Adopting anaerobic fermentation tank to make static culture, its culture medium is RCM culture medium, and its culture time is 48-72 hr and can be up to 2X 108cfu/ml. The three kinds of bacteria liquid are uniformly mixed according to the volume ratio of 5:4:1 to obtain probiotic liquid for later use.
(3) Mixing the raw materials with probiotic liquid: the sterilized raw materials are stirred in a mixer so that the probiotic liquid is uniformly sprayed according to 20 percent by using a 24-hole nozzle arranged in the mixer, and the raw materials are mixed for 4 minutes.
(4) Fermentation: adding distilled water into the mixed materials until the water content is 45%, conveying the materials to an anaerobic fermentation bed by a flood dragon, paving the materials with the thickness of 100cm, fermenting at the initial temperature of 30 ℃, turning the materials once when the temperature reaches 50 ℃, and fermenting for 72 hours. The content of candida utilis in the wet material after 72 hours of fermentation reaches 1.20 multiplied by 10 through detection and monitoring11cfu/g, the content of bacillus subtilis reaches 2 multiplied by 1012cfu/g, clostridium butyricum content up to 3 x 109cfu/g, fermentation was terminated.
(5) And drying and crushing by airflow to obtain a semi-finished product. And (3) granulating the semi-finished product at high temperature to obtain a product, and detecting, measuring and packaging to obtain the laying duck fermented feed.
And (3) result detection: the egg-laying duck fermented feed obtained by the method contains 18.3% of crude protein, 3.7% of glucan and mannan, 3.2% of organic acid and 320mg/kg of B-group microorganisms.
Example 2:
(1) preparing raw materials: uniformly mixing 30% of bean pulp, 2% of sesame pulp, 30% of corn, 10% of bran, 10% of yellow wine lees, 16% of cassava residue, 1.7% of cinnamyl aldehyde and 0.3% of vanillin according to a weight ratio, coarsely crushing the mixture to 40 meshes by using a crusher, conveying the mixture to a conditioner, introducing saturated steam for sterilization and curing, wherein the time in the conditioner is 10min, and cooling to 30 ℃.
(2) Preparing a composite fermentation liquid: candida utilis (A), (B) and (C)Candida utilis) Strain KF-1, Bacillus subtilis (B.subtilis)Bacillus subtilis) Strain KF-2, Clostridium butyricum (C.butyricum) ((C.butyricum))Clostridium butyricum) CICC23847 is respectively activated in a laboratory, and shake flask seeds are manufactured; respectively performing amplification culture, culturing Candida utilis KF-1 in yeast culture medium with inoculum concentration of 10%, culturing temperature of 30 deg.C, stirring at 100r/min and ventilation ratio of 1:1 for 36-72 hr until the concentration of bacteria liquid reaches 2 × 109cfu/ml; bacillus subtilis (A), (B) and (C)Bacillus subtilis) The strain KF-2 is subjected to amplification culture with LB culture medium, the inoculum size is 10%, the culture temperature is 35-40 deg.C, the rotation speed of stirrer is 100r/min, the ventilation ratio is 1:1, and the culture time is 24-48h to 2 × 1010cfu/ml; clostridium butyricum (C)Clostridium butyricum) Adopting anaerobic fermentation tank to make static culture, its culture medium is RCM culture medium, and its culture time is 48-72 hr and can be up to 2X 108cfu/ml. The three kinds of bacteria liquid are uniformly mixed according to the volume ratio of 5:4:1 to obtain probiotic liquid for later use.
(3) Mixing the raw materials with probiotic liquid: the sterilized raw materials are stirred in a mixer so that the probiotics liquid is uniformly sprayed and mixed according to 30 percent by using a 24-hole nozzle arranged in the mixer.
(4) Fermentation: adding distilled water into the mixed materials to water content of 45%, transporting to anaerobic fermentation bed with flood dragon, paving at 150cm thickness, fermenting at 30 deg.C, and allowing the temperature to reachTurning over once at 50 deg.C, and fermenting for 72 hr. The content of candida utilis in the wet material after 72 hours of fermentation reaches 1.40 multiplied by 10 through detection and monitoring11cfu/g, the content of bacillus subtilis reaches 1.8 multiplied by 1012cfu/g, clostridium butyricum content up to 2.9 x 109cfu/g, fermentation was terminated.
(5) And drying and crushing by airflow to obtain a semi-finished product. And (3) granulating the semi-finished product at high temperature to obtain a product, and detecting, measuring and packaging to obtain the laying duck fermented feed.
And (3) result detection: the egg-laying duck fermented feed obtained by the method comprises 20.6% of crude protein, 3.8% of glucan and mannan, 3.0% of organic acid and 311mg/kg of B-group microorganism.
Example 3:
(1) preparing raw materials: uniformly mixing 10% of bean pulp, 3% of sesame pulp, 35% of corn, 20% of bran, 12% of yellow wine lees, 18% of cassava residue, 1.5% of cinnamyl aldehyde and 0.5% of vanillin according to a weight ratio, coarsely crushing the mixture to 40 meshes by using a crusher, conveying the mixture to a conditioner, introducing saturated steam for sterilization and curing, wherein the time in the conditioner is 10min, and cooling to 30 ℃.
(2) Preparing a composite fermentation liquid: candida utilis (A), (B) and (C)Candida utilis) Strain KF-1, Bacillus subtilis (B.subtilis)Bacillus subtilis) Strain KF-2, Clostridium butyricum (C.butyricum) ((C.butyricum))Clostridium butyricum) CICC23847 is respectively activated in a laboratory, and shake flask seeds are manufactured; respectively performing amplification culture, culturing Candida utilis KF-1 in yeast culture medium with inoculum concentration of 10%, culturing temperature of 30 deg.C, stirring at 100r/min and ventilation ratio of 1:1 for 36-72 hr until the concentration of bacteria liquid reaches 2 × 109cfu/ml; bacillus subtilis (A), (B) and (C)Bacillus subtilis) The strain KF-2 is subjected to amplification culture with LB culture medium, the inoculum size is 10%, the culture temperature is 35-40 deg.C, the rotation speed of stirrer is 100r/min, the ventilation ratio is 1:1, and the culture time is 24-48h to 2 × 1010cfu/ml; clostridium butyricum (C)Clostridium butyricum) Adopting anaerobic fermentation tank to make static culture, its culture medium is RCM culture medium, and its culture time is 48-72 hr and can be up to 2X 108cfu/ml. Uniformly mixing the three bacteria liquids according to the volume ratio of 5:4:1 to obtain the probioticsAnd (6) liquid for later use.
(3) Mixing the raw materials with probiotic liquid: the sterilized raw materials are stirred in a mixer so that the probiotics liquid is uniformly sprayed and mixed according to 10 percent by using a 24-hole nozzle arranged in the mixer.
(4) Fermentation: adding distilled water into the mixed materials until the water content is 45%, conveying the materials to an anaerobic fermentation bed by a flood dragon, paving the materials with the thickness of 50cm, fermenting at the initial temperature of 30 ℃, turning the materials once when the temperature reaches 50 ℃, and fermenting for 72 hours. The content of candida utilis in the wet material after 72 hours of fermentation reaches 1.20 multiplied by 10 through detection and monitoring11cfu/g, the content of bacillus subtilis reaches 2 multiplied by 1012cfu/g, clostridium butyricum content up to 3 x 109cfu/g, fermentation was terminated.
(5) And drying and crushing by airflow to obtain a semi-finished product. And (3) granulating the semi-finished product at high temperature to obtain a product, and detecting, measuring and packaging to obtain the laying duck fermented feed.
And (3) result detection: the egg-laying duck fermented feed obtained by the method contains 19.8% of crude protein, 3.9% of glucan and mannan, 3.4% of organic acid and 336mg/kg of B-group microorganisms.
Example 4:
animal experiments were carried out on the fermented feed for laying ducks by 200000 agricultural development Limited in Jinhua, Zhejiang.
6000 shaoxing sheldrakes of 255 days old in the egg laying period are selected and randomly divided into two groups, wherein each group is 3 in number of repetitions, and each repetition is 1000 in number. The test groups and the control groups are in the same house and are distributed in a cross way, so that each treatment group has the same test environment. The experimental group is added with 5 percent of the laying duck fermented feed of the invention on the basis of the basic amount, and the control group is basic daily ration. And (5) performing conventional management according to the laying duck farm.
Observation indexes are as follows: feed intake, egg production, egg weight, egg breaking condition and the like, and collecting excrement for detecting conventional indexes such as protein and the like.
The influence of the laying duck fermented feed on the production performance of the Shaoxing sheldrake is shown in table 1.
Table 1: influence of laying duck fermented feed on production performance of Shaoxing sheldrake
As can be seen from the table 1, after the feed is fermented by using the product of the invention, the laying rate of laying ducks is improved by 5.38, the average egg weight is increased by 3.16, the feed-egg ratio is reduced by 0.1, and the production performance of the laying ducks is obviously improved. The culture benefit is very obvious.
The effect of the fermented feed for laying ducks on the feces of the Shaoxing sheldrake is shown in Table 2.
Table 2: influence of laying duck fermented feed on excrement of Shaoxing sheldrake
As can be seen from the table 2, after the laying duck fermented feed is used, the crude protein of the excrement is reduced from 25.06% to 23.16%, and the ash content and the water content are not obviously different, which shows that the digestion performance of the laying duck is effectively improved after the laying duck fermented feed is used.
Similar results to those in the above-described test were obtained and the same effects were obtained by performing the same test as in the present example using the yeast-derived fermented feeds obtained in examples 2 and 3. Can improve the immunity of the laying ducks, improve the digestive ability of the laying ducks, improve the laying rate and increase the weight of eggs.
Claims (10)
1. The laying duck fermented feed is characterized by being prepared through the following steps:
(1) uniformly mixing 10-30% of bean pulp, 2-5% of sesame pulp, 12-35% of corn, 10-20% of bran, 5-15% of yellow wine lees, 5-20% of cassava dregs, 0.5-2% of cinnamaldehyde and 0.1-0.5% of vanillin, roughly crushing to 40 meshes, completely passing through the coarse grains, sterilizing and curing by saturated steam for 10min, and cooling to 30 ℃;
(2) respectively activating Candida utilis KF-1, Bacillus subtilis strain KF-2 and Clostridium butyricum CICC23847, preparing shake flask seeds, inoculating to culture medium, respectively expanding and culturing to obtain strain liquid with concentration of 2 × 1010After cfu/ml is finished, candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 are cultured according to the volume ratio of 5:41, mixing to obtain probiotic liquid;
(3) spraying the probiotic liquid obtained in the step (2) to the raw material obtained in the step (1) through a full-automatic nozzle, and fully mixing, wherein the addition amount of the probiotic liquid is 10% -32%;
(4) and (3) performing fermentation treatment, drying by airflow after the fermentation is finished, performing superfine grinding to break the wall, sieving by a 80-mesh sieve, and performing granulation and extrusion to obtain the laying duck fermented feed.
2. The fermented feed for laying ducks of claim 1, wherein the conditions for the expanded culture of candida utilis KF-1 in the step (2) are as follows: adopting a yeast culture medium to culture samples, wherein the inoculation amount is 10%, the culture temperature is 30 ℃, the culture time is 36-72h, the rotating speed of a stirrer is 100r/min, the ventilation ratio is 1:1, and the amplification culture conditions of the bacillus subtilis strain KF-2 are as follows: adopting LB culture medium for culture, the inoculum size is 10%, the culture temperature is 35-40 ℃, the culture time is 24-48h, the rotating speed of a stirrer is 100r/min, and the ventilation ratio is 1: 1.
3. The fermented feed for laying ducks according to claim 1, wherein the expanded culture conditions of clostridium butyricum CICC23847 in the step (2) are as follows: standing in anaerobic fermenter, and culturing in RCM culture medium for 48-72 hr.
4. The fermented feed for laying ducks of claim 5, wherein the fermentation treatment conditions in the step (3) are as follows: anaerobic solid state fermentation is adopted, the spreading thickness is 50-150cm, the fermentation time is 48-72h, and the fermentation is carried out once when the temperature reaches 50 ℃.
5. A preparation method of a laying duck fermented feed is characterized by comprising the following steps:
(1) uniformly mixing 10-30% of bean pulp, 2-5% of sesame pulp, 12-35% of corn, 10-20% of bran, 5-15% of yellow wine lees, 5-20% of cassava dregs, 0.5-2% of cinnamaldehyde and 0.1-0.5% of vanillin, roughly crushing to 40 meshes, completely passing through the coarse grains, sterilizing and curing by saturated steam for 10min, and cooling to 30 ℃;
(2) mixing Candida utilis KF-1 and Bacillus subtilisRespectively activating Bacillus subtilis KF-2 and Clostridium butyricum CICC23847, preparing shake flask seeds, inoculating to culture medium, respectively culturing under amplification to obtain liquid with concentration of 2 × 1010After cfu/ml is finished, mixing candida utilis KF-1, bacillus subtilis strain KF-2 and clostridium butyricum CICC23847 according to the volume ratio of 5:4:1 to obtain probiotic liquid;
(3) spraying the probiotic liquid obtained in the step (2) to the raw material obtained in the step (1) through a full-automatic nozzle, and fully mixing, wherein the addition amount of the probiotic liquid is 10% -32%;
(4) and (3) performing fermentation treatment, drying by airflow after the fermentation is finished, performing superfine grinding to break the wall, sieving by a 80-mesh sieve, and performing granulation and extrusion to obtain the laying duck fermented feed.
6. The fermented feed for laying ducks of claim 5, wherein the conditions for the expanded culture of the candida utilis KF-1 in the step (2) are as follows: the yeast culture medium is adopted for sample culture, the inoculation amount is 10%, the culture temperature is 30 ℃, the culture time is 36-72h, the rotating speed of a stirrer is 100r/min, and the ventilation ratio is 1: 1.
7. The fermented feed for laying ducks according to claim 5, wherein the conditions for the expanded culture of the bacillus subtilis strain KF-2 in the step (2) are as follows: adopting LB culture medium for culture, the inoculum size is 10%, the culture temperature is 35-40 ℃, the culture time is 24-48h, the rotating speed of a stirrer is 100r/min, and the ventilation ratio is 1: 1.
8. The fermented feed for laying ducks according to claim 5, wherein the expanded culture conditions of Clostridium butyricum CICC23847 in the step (2) are as follows: standing in anaerobic fermenter, and culturing in RCM culture medium for 48-72 hr.
9. The fermented feed for laying ducks of claim 5, wherein the fermentation treatment conditions in the step (3) are as follows: anaerobic solid state fermentation is adopted, the spreading thickness is 50-150cm, the fermentation time is 48-72h, and the fermentation is carried out once when the temperature reaches 50 ℃.
10. The use of the fermented feed for laying ducks according to any one of claims 1-4 in enhancing the digestive performance, enhancing the immunity, increasing the laying rate and increasing the egg weight of laying ducks.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010147726.3A CN111296673A (en) | 2020-03-05 | 2020-03-05 | Laying duck fermented feed and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010147726.3A CN111296673A (en) | 2020-03-05 | 2020-03-05 | Laying duck fermented feed and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111296673A true CN111296673A (en) | 2020-06-19 |
Family
ID=71152273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010147726.3A Pending CN111296673A (en) | 2020-03-05 | 2020-03-05 | Laying duck fermented feed and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111296673A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383872A (en) * | 2021-07-13 | 2021-09-14 | 上海红马饲料有限公司 | Duck feed formula capable of preventing digestive diseases and preparation process |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468594A (en) * | 2013-07-31 | 2013-12-25 | 浙江科峰生物技术有限公司 | Candidautilis strain and application thereof |
| CN107535671A (en) * | 2017-09-27 | 2018-01-05 | 浙江大学 | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate |
| CN109007247A (en) * | 2018-07-27 | 2018-12-18 | 望江县袁源农业有限公司 | A kind of preparation method of laying duck nutrient fodder |
| CN110122658A (en) * | 2019-05-14 | 2019-08-16 | 江苏祥豪实业股份有限公司 | A kind of fermentative feedstuff of microbe and its preparation method and application |
-
2020
- 2020-03-05 CN CN202010147726.3A patent/CN111296673A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468594A (en) * | 2013-07-31 | 2013-12-25 | 浙江科峰生物技术有限公司 | Candidautilis strain and application thereof |
| CN107535671A (en) * | 2017-09-27 | 2018-01-05 | 浙江大学 | Improve the microbial fermentation yellow wine lees feed and preparation method of ruminal protein utilization rate |
| CN109007247A (en) * | 2018-07-27 | 2018-12-18 | 望江县袁源农业有限公司 | A kind of preparation method of laying duck nutrient fodder |
| CN110122658A (en) * | 2019-05-14 | 2019-08-16 | 江苏祥豪实业股份有限公司 | A kind of fermentative feedstuff of microbe and its preparation method and application |
Non-Patent Citations (1)
| Title |
|---|
| 阮栋等: "发酵饲料对蛋鸭产蛋性能、蛋品质、肠道消化酶活性及免疫功能的影响", 《动物营养学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113383872A (en) * | 2021-07-13 | 2021-09-14 | 上海红马饲料有限公司 | Duck feed formula capable of preventing digestive diseases and preparation process |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105661011B (en) | Functional biological protein feed leavening agent and fermented protein feed | |
| CN101711548B (en) | Fermented feed containing sodium humate and process and application thereof | |
| CN106260504B (en) | Method for producing microbial fermentation wet feed by using beer yeast paste | |
| CN107801837B (en) | Biological fermentation process for forage-based utilization of chicken manure | |
| CN102805209A (en) | Synbiotics feed additive for beasts and birds and application thereof | |
| CN110591943B (en) | Bacillus subtilis capable of producing complex enzyme, composition and application thereof | |
| CN115287202B (en) | Kluyveromyces marxianus and application thereof in preparation of functional feed | |
| CN103859155A (en) | Refining method for probiotics-containing cassava feed | |
| CN108541805A (en) | The reuse method and fermentate of a kind of clostridium butyricum fermentation waste water and its application | |
| CN103829042A (en) | Production method of multi-vitamin active cassava protein feed | |
| CN106615658A (en) | Method for preparing fermentation bed padding by recycling froggrass | |
| CN112385737A (en) | Preparation method of high-mannan-oligosaccharide palm meal fermented feed | |
| CN111466481A (en) | Liquid bacterium solution for fermenting liquid piglet feed, preparation method and application of liquid fermented piglet feed | |
| CN106635902A (en) | Bacillus coagulans and application thereof | |
| CN115067419A (en) | Application of feed additive and preparation method of earthworm fermentation liquor | |
| CN111728082A (en) | Natural plant micro-ecological feed additive for fermented feed of laying hens and preparation method thereof | |
| CN106306426A (en) | Novel process for corn silage | |
| CN106035990B (en) | Method for preparing biological feed by solid-state enzymolysis fermentation of citrus pulp, product and application thereof | |
| CN112961806B (en) | Bacillus coagulans for high-yield lactic acid, biological fermentation feed and preparation method and application thereof | |
| CN103719537A (en) | Nonreactive biological fermented feed and preparation method thereof | |
| CN113773996A (en) | Preparation method and application of feeding bacillus subtilis | |
| CN113414226A (en) | Kitchen waste leavening agent and preparation method and application thereof | |
| KR20000073915A (en) | Probiotics For Feed Additives | |
| CN111296673A (en) | Laying duck fermented feed and application thereof | |
| KR101252134B1 (en) | Feed additives for promoting growth of cattle and process for the preparation of feed for breeding cattle using the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200619 |