CN1973659B - Prawn feed additive - Google Patents
Prawn feed additive Download PDFInfo
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- CN1973659B CN1973659B CN2006101223055A CN200610122305A CN1973659B CN 1973659 B CN1973659 B CN 1973659B CN 2006101223055 A CN2006101223055 A CN 2006101223055A CN 200610122305 A CN200610122305 A CN 200610122305A CN 1973659 B CN1973659 B CN 1973659B
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- prawn
- feed
- feed additive
- prawn feed
- herbal medicine
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- 241000238557 Decapoda Species 0.000 title claims abstract description 35
- 239000003674 animal food additive Substances 0.000 title claims abstract description 11
- 241000411851 herbal medicine Species 0.000 claims abstract description 15
- 239000000843 powder Substances 0.000 claims abstract description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims description 18
- 150000001875 compounds Chemical class 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- 239000011343 solid material Substances 0.000 claims description 7
- 239000000654 additive Substances 0.000 claims description 6
- 230000000996 additive effect Effects 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000005469 granulation Methods 0.000 claims description 4
- 230000003179 granulation Effects 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 240000004510 Agastache rugosa Species 0.000 claims description 3
- 241001061264 Astragalus Species 0.000 claims description 3
- 235000010110 Astragalus glycyphyllos Nutrition 0.000 claims description 3
- 244000247747 Coptis groenlandica Species 0.000 claims description 3
- 235000002991 Coptis groenlandica Nutrition 0.000 claims description 3
- 241000576429 Forsythia suspensa Species 0.000 claims description 3
- 239000009636 Huang Qi Substances 0.000 claims description 3
- 235000006533 astragalus Nutrition 0.000 claims description 3
- 239000010231 banlangen Substances 0.000 claims description 3
- 239000002775 capsule Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 9
- 239000003814 drug Substances 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 3
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 4
- 239000000463 material Substances 0.000 abstract 3
- 239000007787 solid Substances 0.000 abstract 2
- 241001529821 Agastache Species 0.000 abstract 1
- 241000037740 Coptis chinensis Species 0.000 abstract 1
- 241000334160 Isatis Species 0.000 abstract 1
- 244000137773 Viola philippica Species 0.000 abstract 1
- 229940107666 astragalus root Drugs 0.000 abstract 1
- 102000038379 digestive enzymes Human genes 0.000 abstract 1
- 108091007734 digestive enzymes Proteins 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 241000238553 Litopenaeus vannamei Species 0.000 description 8
- 239000004382 Amylase Substances 0.000 description 5
- 102000013142 Amylases Human genes 0.000 description 5
- 108010065511 Amylases Proteins 0.000 description 5
- 235000019418 amylase Nutrition 0.000 description 5
- 210000004185 liver Anatomy 0.000 description 5
- 108091005804 Peptidases Proteins 0.000 description 4
- 230000037396 body weight Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 102000035195 Peptidases Human genes 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000004568 cement Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 230000006862 enzymatic digestion Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 238000000247 postprecipitation Methods 0.000 description 2
- 235000019833 protease Nutrition 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical compound OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 229920002261 Corn starch Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000004567 concrete Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 229940099112 cornstarch Drugs 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000010200 folin Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention discloses one kind of prawn feed additive. The prawn feed additive is compounded with Chinese herbal medicine powder in 8-12 weight portions and solid lichen-forming bacillus material in 12-18 weight portions. The Chinese herbal medicine powder is prepared with isatis root, forthythia, astragalus root, agastache, Viola yedoensis and Chinese goldthread and through stoving andcrushing. The solid lichen-forming bacillus material contains lichen-forming bacillus in 10<9> CFU/g. The prawn feed additive is added into prawn feed in the added amount of 0.15-0.50 wt% before the feed is pelletized into 1.5 mm size grains. By means of the synergistic effects of the Chinese medicine materials and the lichen-forming bacillus, the prawn feed additive has the functions of raising the activity of digestive enzyme, promoting the growth of prawn and raising the survival rate of prawn.
Description
Technical field
The present invention relates to a kind of prawn feed additive.
Technical background
Along with the fast development of shrimp culture industry and improving constantly of intensive culture degree, the disease problem is serious day by day.In traditional pest control method, antibiotic and chemicals are used as main means and use in a large number, have caused pathogen to develop immunity to drugs, and result of treatment descends; Simultaneously, because medicine residual, have a strong impact on the health and the Environmental security of consumer groups.Because the aquatic products medicament residue exceeds standard, and causes products export limited, has had a strong impact on the competitiveness of China's aquatic products, has hindered the developing of domestic and international market, has caused enormous economic loss.
Summary of the invention
The object of the present invention is to provide a kind of prawn feed additive, can improve the digestive enzyme activity of prawn, the growth of promotion cultured prawn significantly, reduce the disease problem of prawn culturing.
Prawn feed additive of the present invention is composited by the proportioning of following raw material with parts by weight:
Chinese herbal medicine powder 8-12 bacillus licheniformis solid material 12-18.
Compound Chinese herbal medicine powder of the present invention is composited after being pulverized by the Radix Isatidis that waits weight portion, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and coptis drying.
The bacillus licheniformis flora is 10 in the bacillus licheniformis complex solid material of the present invention
9CFU/g.
The percentage by weight that adds in prawn feed of compound additive of the present invention is 0.15%-0.50%, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
Beneficial effect of the present invention: the present invention adds an amount of Chinese herbal medicine and bacillus licheniformis in prawn feed, by their synergy, can obviously improve digestive enzyme activity, promote the growth of prawn and improve the prawn survival rate, have good economic benefit and social benefit, help the sustainable sound development of prawn culturing, meet the development strategy trend of aquatic feeds.
The specific embodiment
Radix Isatidis, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and the coptis of weight portion such as get, concrete preparation process is: bulk drug → first drying → secondary drying → coarse crushing → ultramicro grinding → sieve → composite, make compound Chinese herbal medicine powder.
Get culture medium (composition: cornstarch 2%, peptone 2.5%, corn steep liquor 1.5%, NaH2PO40.8%; PH7.2-7.4) → sterilize (121 ℃, 30min) → cooling (30 ℃) → (30 ℃ of (inoculum concentration 5%) → seed tank culture of inoculation, 18h) → enlarge and cultivate (30 ℃, 24h) → centrifugal → be written into carrier (wheat bran) → drying → pulverizing, make bacillus licheniformis complex solid material, the bacillus licheniformis flora is 10 in the above-mentioned bacillus licheniformis complex solid material
9CFU/g.
Take by weighing the above-mentioned raw material that makes, mix, make the prawn feed additive, wherein Chinese herbal medicine powder 8-12 with the proportioning of parts by weight; Bacillus licheniformis solid material 12-18.
Above-mentioned compound additive directly adds in the prawn feed, and addition is the 0.15%-0.50% of prawn feed weight, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
The experiment of feeding prawn with the present invention is as follows:
Experimental result adopts average ± standard deviation to represent, uses one-way analysis of variance (ANOVA) statistics, when having significant difference between the ANOVA demonstration is handled, uses Duncan ' s multiple ratio with the analysis bank differences.SPSS 11.5 statistical softwares are used in all statistical analyses, are the significance of difference with P<0.05.
Prepare five kinds of prawn feeds, feed A: prawn basal feed; Feed B: in basal feed, add 0.2% above-mentioned compound Chinese herbal medicine powder; Feed C: bacillus licheniformis feed group, in basal feed, add 0.30% above-mentioned bacillus licheniformis; Feed D: in basal feed, add 0.25% Chinese herbal medicine of the present invention and bacillus licheniformis compound additive; Feed E: in basal feed, add 0.50% Chinese herbal medicine of the present invention and bacillus licheniformis compound additive.The particle that particle diameter is 1.5mm is made in granulation behind the mixing.
With above-mentioned prawn mixed feed, fed initial body weight 2.03g Environment of Litopenaeus vannamei Low (Litopenaeus vannamei) 70 days.In indoor cement circle pond 2.0m
3Grouping is carried out.Culturing water filters through post precipitation.Shrimp 80 tails are supported in every pond, and every group of 3 in feed is parallel.Keep continuous charge.The breeding process water temperature is 19.6-25.2 ℃, salinity 30~31 ‰.
Culture efficiency sees Table 1.(feed A) compares with control group, in the feed separately or associating wherein add bacillus licheniformis and Chinese herbal medicine, can both promote the growth of prawn.Especially, feed D group is significantly higher than other each group, does not compare with the Chinese herbal medicine group with adding bacillus licheniformis, and rate of body weight gain improves 35%.
The throw something and feed Environment of Litopenaeus vannamei Low culture efficiency of DIFFERENT FEED of table 1
Group | A | B | C | D | E |
Survival rate (%) rate of body weight gain (%) | 95.83±1.44 116.89±3.45 a | 97.92±1.91 136.17±5.02 b | 97.50±1.25 127.89±3.55 ab | 98.33±0.73 161.65±17.68 c | 96.67±1.91 137.08±11.66 b |
(average ± SD) there is significant difference (p<0.05) in expression to same line data between the different persons of subscript letter
With above-mentioned prawn mixed feed, fed initial body weight 1.9g Environment of Litopenaeus vannamei Low (Litopenaeus vannamei) 56 days.In indoor cement pit (1.0 * 1.0 * 1.0m
3) divide into groups to carry out, culture water and filter through post precipitation.Shrimp 100 tails are supported in every pond, and every group of 3 in feed is parallel.Keep continuous charge.The breeding process water temperature is 19.6-25.2 ℃, salinity 30~31 ‰.Experimental session was taken a sample once every 2 weeks, and shrimp 10 tails are got in each every pond, and the liver pancreas of taking-up is put in-70 ℃ of ultra low temperature freezers and preserves until the digestive ferment analysis.
The mensuration of prolease activity is substrate with reference to Anson (1938) with the casein, and Folin reagent is a developer; The mensuration of amylase activity is with reference to Bernfeld (1955), with soluble starch as substrate, 3,5-dinitrosalicylic acid developer cessation reaction; The mensuration of the white concentration of enzyme liquid eggs is made standard with bovine serum albumin, measures (Bradford, 1976) with the Coomassie brilliant blue method.
The proteinase activity of liver and amylase activity analysis see Table 2 and table 3 respectively.In all 4 sub-samplings, the independent and common interpolation of bacillus licheniformis and Chinese herbal medicine has all improved the protease and the amylase activity of Environment of Litopenaeus vannamei Low liver pancreas.Especially, the 2nd week, the protease and the amylase activity of the additive group of the present invention (feed D) of interpolation 0.25% all were higher than control group (feed A) more than 60% when taking a sample, and the result of 4 sub-samplings on average improves 30%.
The throw something and feed variation of Environment of Litopenaeus vannamei Low liver proteinase activity (IU/mg albumen) of DIFFERENT FEED of table 2
Group | A | B | C | D | E |
6 all 8 weeks of 4 weeks in 2 weeks | 0.082±0.005 a 0.102±0.004 0.142±0.006 a 0.096±0.019 | 0.102±0.010 ab 0.116±0.016 0.142±0.009 a 0.103±0.007 | 0.100±0.010 ab 0.110±0.009 0.155±0.007 ab 0.127±0.026 | 0.1 32±0.030 b 0.118±0.015 0.1 66±0.007 b 0.116±0.009 | 0.114±0.017 ab 0.116±0.019 0.151±0.008 ab 0.105±0.007 |
(average ± SD) there is significant difference (p<0.05) in expression to same line data between the different persons of subscript letter
The throw something and feed variation of Environment of Litopenaeus vannamei Low liver amylase activity (IU/mg albumen) of DIFFERENT FEED of table 3
Group | A | B | C | D | E |
6 all 8 weeks of 4 weeks in 2 weeks | 0.67 1±0.094 a 0.864±0.175 1.130±0.022 a 0.914±0.111 a | 0.787±0.123 a0.925±0.1161.242±0.113 abc0.91 3±0.022 a | 0.883±0.104 ab 0.986±0.080 1.337±0.054 bc 1.192±0.154 b | 1.131±0.260 b 0.974±0.144 1.377±0.021 c 0.973±0.062 a | 0.876±0.139 ab 0.978±0.091 1.176±0.143 ab 0.942±0.050 a |
(average ± SD) there is significant difference (p<0.05) in expression to same line data between the different persons of subscript letter
Claims (2)
1. prawn feed additive is characterized in that being composited by the proportioning of following raw material with parts by weight:
Chinese herbal medicine powder 8-12, bacillus licheniformis solid material 12-18;
Described compound Chinese herbal medicine powder is composited after being pulverized by the Radix Isatidis that waits weight portion, the capsule of weeping forsythia, the Radix Astragali, wrinkled giant hyssop, prairie milk vetch and coptis drying;
The bacillus licheniformis flora is 10 in the described bacillus licheniformis complex solid material
9CFU/g.
2. an application rights requires the method for 1 additive, it is characterized in that the addition in prawn feed is 0.15%-0.50%, and the particle that particle diameter is 1.5mm is made in granulation behind the mixing.
Priority Applications (1)
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CN2006101223055A CN1973659B (en) | 2006-09-22 | 2006-09-22 | Prawn feed additive |
Applications Claiming Priority (1)
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CN2006101223055A CN1973659B (en) | 2006-09-22 | 2006-09-22 | Prawn feed additive |
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CN1973659A CN1973659A (en) | 2007-06-06 |
CN1973659B true CN1973659B (en) | 2010-07-21 |
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CN2006101223055A Expired - Fee Related CN1973659B (en) | 2006-09-22 | 2006-09-22 | Prawn feed additive |
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101438772B (en) * | 2008-12-26 | 2011-06-08 | 集美大学 | Compound protein feed for shrimp and method for preparing the same |
CN103518996A (en) * | 2013-09-03 | 2014-01-22 | 孙文秀 | Shrimp feed additive |
CN105360715A (en) * | 2015-11-09 | 2016-03-02 | 杭州亿尔生物科技有限公司 | Traditional Chinese medicine microbial composite additive for adjusting shrimp body color |
CN105249022A (en) * | 2015-11-11 | 2016-01-20 | 浙江锦天生物科技有限公司 | Compound micro-ecological feed additives for litopenaeus vannamei |
CN105433061A (en) * | 2015-12-23 | 2016-03-30 | 广州聚注专利研发有限公司 | Microbial fish feed additive and preparation method thereof |
CN113046275B (en) * | 2021-04-27 | 2022-03-29 | 中国海洋大学 | Bacillus used for prawn feed and fermented Chinese herbal medicine preparation used for prawn feed |
Citations (1)
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CN1620892A (en) * | 2004-12-10 | 2005-06-01 | 中国海洋大学 | Compound microecological preparation for prawn |
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CN1620892A (en) * | 2004-12-10 | 2005-06-01 | 中国海洋大学 | Compound microecological preparation for prawn |
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