CN105249022A - Compound micro-ecological feed additives for litopenaeus vannamei - Google Patents
Compound micro-ecological feed additives for litopenaeus vannamei Download PDFInfo
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- CN105249022A CN105249022A CN201510770286.6A CN201510770286A CN105249022A CN 105249022 A CN105249022 A CN 105249022A CN 201510770286 A CN201510770286 A CN 201510770286A CN 105249022 A CN105249022 A CN 105249022A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses compound micro-ecological feed additives for litopenaeus vannamei. The additives are natural in source, low in cost and capable of effectively enhancing basic feed nutritional value, improving immunity of litopenaeus vannamei and ensuring the health of litopenaeus vannamei, and particularly has a quite excellent effect on preventing and treating larval bacteriemia. The micro-ecological feed additives are natural in material resource and free of toxic and side effects, can enhance the constitution, immunity and reflection ability of litopenaeus vannamei, are free of residues and do no harm to human bodies. The compound micro-ecological feed additives contain various Chinese herbal medicine effective constituents, active bacteria, enzymes and the like, have a quite excellent effect for litopenaeus vannamei, are long in shelf life, and can be stored for 24 months at a room temperature, and the survival rate of active bacteria is still 90% or higher.
Description
Technical field
The present invention relates to a kind of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, belong to feed additive field.
Background technology
Feed addictive refers at Feed Manufacturing processing, a small amount of or micro substance that adds in use procedure, in feed consumption seldom but effect significantly.Feed addictive is the raw material that modern feed industry must use, and to strengthening basal feed nutritive value, improve Environment of Litopenaeus vannamei Low production performance, ensure that Environment of Litopenaeus vannamei Low is healthy, save feed cost, improving the aspects such as livestock products quality has obvious effect.
In recent years, along with the development of high density, intensive aquaculture mode, the generation of disease is also more and more frequent.In order to disease preventing and treating, conventional method uses chemicals and antibiosis (bacterium) element in a large number.But chemicals and antibioticly to reuse in a large number, causes the generation of bacterial drug resistance, does great damage to ecological environment, but also threaten the health of the mankind.Seek chemicals and antibiotic substitute and become inevitable, so the material that vaccine, immunopotentiator and probio etc. can improve immunity of organism level grows up gradually, and progressively apply in aquaculture.
Occur now that additive for microbe feedstuff is a kind of microbial product replacing or balance one or more fungus strains in the Environment of Litopenaeus vannamei Low ecosystem.Say in the narrow sense, it is that one can excite own beneficial culture propagation to increase, the microbial product of withstand deleterious fungus strain growth simultaneously.
Environment of Litopenaeus vannamei Low (Litopenaeusvannamei), also known as Penaeus Vannmei (WhitePrawn), call white pin shrimp, Litoenaeus vannamei, country of origin Ecuador, waters, Pacific coast, South America in original producton location, the growth climate zone torrid zone, subtropical zone, warm temperate zone, marine site, temperate zone, be distributed in the middle part of West Coast, the Pacific Ocean to the Gulf of Mexico, life cycle 1 year.Environment of Litopenaeus vannamei Low is that it has, and growth is fast, resistance against diseases is strong for one of three large improved seeds that on our times, cultured output is the highest (other two kinds is Penaeus monodon, Chinese prawn), and fanning economics is remarkable.Rapidly throughout the whole nation after Environment of Litopenaeus vannamei Low introduced China in 1988.Through the development of more than 20 years, the cultural technique of Environment of Litopenaeus vannamei Low was gradually ripe, and output increases year by year.Since calendar year 2001, China's cultivation Penaeus Vannmei area and output occupy the first place of prawn culturing always, and its cultivation amount has reached more than 70% of China's prawn culturing amount, have also become simultaneously and have cultivated the highest prawn kind of largest output in the world.It is reported, Chinese Environment of Litopenaeus vannamei Low year cultivation nearly 1,000,000 tons of total amount, wherein nearly 200,000 tons of sea-farming, nearly 800,000 tons of freshwater aquiculture.
The present invention aims to provide a kind of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, and its source is natural, with low cost, it can efficient hardening basal feed nutritive value, improve the immunity of Environment of Litopenaeus vannamei Low, ensure that it is healthy, particularly have extremely outstanding effect to control young bacteremia.
Summary of the invention
Based on the technical problem that background technology exists, the present invention is directed to background technology Problems existing, a kind of Environment of Litopenaeus vannamei Low composite microorganism type forage additives is provided, its source is natural, with low cost, it can efficient hardening basal feed nutritive value, improves the immunity of Environment of Litopenaeus vannamei Low, ensure that it is healthy, particularly have extremely outstanding effect to control young bacteremia.
Object of the present invention is achieved through the following technical solutions:
A preparation method for Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.2-0.3 part tourmaline powder, 7-8 part hawthorn, 0.5-0.8 part leaves of Hawthorn, 2-3 part folium cortex eucommiae, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 80-85min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 10-15 part traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
The compatibility of Chinese herbal feed additive both can select simple, also the compound that multi-flavor medicine forms can be selected, but not the simple rough combination of some medicines, without " method " nothing " side " nothing " compatibility ", but under the guidance of Chinese veterinarian's theory, according to inherent laws and the feature thereof of Chinese veterinarian's pharmacy, on theory contact practical basis, consider that compatibility of drugs gets up to use by the performance of modern medicines itself selectively, give full play to the mass action of medicine.Medicine for the concrete condition of Environment of Litopenaeus vannamei Low, according to certain principle, should combine, just defines prescription by the prescription of Chinese herbal medicine selectively.This principle is said in " Plain Questions to very will discuss greatly ": " main disease be monarch, what help monarch is minister, answer minister for making.Monarch one minister two, system also little; Monarch one minister three helps five, among system also; Monarch one minister three helps nine, makes also big." principle of Here it is herbal prescription.The principles of formulating prescriptions of Chinese herbal medicine may be summarized to be monarch, minister, help, make, be referred to as now more main and auxiliary, help, make four aspects.According to characteristic and the major function of various Chinese herbal medicine, adopt extraction, its active component of concentrated natural plant, screening is optimization of C/C composites also, improves and optimizes traditional Chinese herbal and crude drugs preparations technique.
The present invention is on the basis of Chinese traditional herbs theory, in conjunction with modernization bioprocess technology, preparing can efficient hardening basal feed nutritive value, improve Environment of Litopenaeus vannamei Low immunity, ensure that the complex microecological feed of Environment of Litopenaeus vannamei Low health adds, and its long shelf-life, under normal temperature, storage can reach 24 months, and the survival rate of viable bacteria is still more than 90%.
Usefulness of the present invention is:
1) microbial forage additive of the present invention is drawn materials natural without any side effects, not only can strengthen the physique of Environment of Litopenaeus vannamei Low, immunity, stress ability, and noresidue, harmless.
2) composite microorganism type forage additives of the present invention, it contains multiple Chinese herbal medicine effective ingredients, active bacteria, enzyme etc., to Environment of Litopenaeus vannamei Low excellent, particularly has extremely outstanding effect to control young bacteremia.
3) long shelf-life, under normal temperature, storage can reach 24 months, and the survival rate of viable bacteria is still more than 90%.
Detailed description of the invention
Embodiment 1:
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.2 part of tourmaline powder, 8 portions of hawthorn, 0.5 part of leaves of Hawthorn, 3 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 80min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 15 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 2:
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.3 part of tourmaline powder, 7 portions of hawthorn, 0.8 part of leaves of Hawthorn, 2 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 85min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 10 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 3:
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.2 part of tourmaline powder, 8 portions of hawthorn, 0.6 part of leaves of Hawthorn, 3 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 81min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 14 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 4:
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.3 part of tourmaline powder, 7 portions of hawthorn, 0.7 part of leaves of Hawthorn, 2 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 84min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 11 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 5:
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.2 part of tourmaline powder, 8 portions of hawthorn, 0.6 part of leaves of Hawthorn, 3 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 82min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 13 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 6:
Each proportioning of the present invention's formula is extremely important, and deviation does not just reach desirable effect a little, and contrast test is only exemplary experiment.
The preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.1 part of tourmaline powder, 9 portions of hawthorn, 0.4 part of leaves of Hawthorn, 5 parts of folium cortex eucommiaes, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 79min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder;
(3) get 16 parts of traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
Embodiment 7 feeds effect test and appraisal:
Buying Environment of Litopenaeus vannamei Low shrimp seedling, assigns in 18 cement pits, each cement pit 500 tail prawn at random by experiment shrimp.By feed difference, experiment shrimp is divided into 6 groups, often organizes 3 repetitions.Feed adopts conventional prawn feed.Experimental session to be thrown something and fed feed 2 times every day, adds the biology feed additive of the present invention of 0.2% in basal diet.
Throw something and feed at the 9:00 ~ 10:00 in the morning and the 16:00 ~ 17:00 in afternoon respectively, feeding volume is 3% ~ 5% of prawn body weight.
Young bacteremia prevention effect:
Young bacteremia is generally and infects vibrios, pseudomonad, Aeromonas.Body colour is opaque, and phototaxis is poor.Acute infection colony, is sunken to the bottom in hydrostatic, dead fast; The colony that the course of disease is slow, its body surface and appendage can stick the dirts such as many unicellular algas, set class infusorian or organic debris.
After feeding 60 days, in cement pit, drop into 20 young bacteremias in every pond suffer from shrimp, then continue the death rate of whole pond shrimp after statistics 14d.Result is as shown in the table.
Table 1
As can be seen here, the biology feed additive of the present invention adding 0.2% in basal diet can play good facilitation to the growth of Environment of Litopenaeus vannamei Low, can significantly improve its immunity, especially greatly strengthen the resistivity of young bacteremia.
Visible, composite microorganism type forage additives source of the present invention is natural, with low cost, can efficient hardening basal feed nutritive value, improve Environment of Litopenaeus vannamei Low production performance, ensure that Environment of Litopenaeus vannamei Low is healthy, save feed cost, and preserve 24 months under normal temperature, its number of viable is still greater than 90%.
The above; be only the present invention's preferably detailed description of the invention; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses; be equal to according to technical scheme of the present invention and inventive concept thereof and replace or change, all should be encompassed within protection scope of the present invention.
Claims (4)
1. a preparation method for Environment of Litopenaeus vannamei Low composite microorganism type forage additives, is characterized in that, step is as follows:
As described belowly be mass parts;
(1) traditional Chinese medicine powder is prepared:
Get 0.2-0.3 part tourmaline powder, 7-8 part hawthorn, 0.5-0.8 part leaves of Hawthorn, 2-3 part folium cortex eucommiae, 0.2 part of Radix Astragali, 0.2 portion of motherwort, 0.5 portion of Radix Codonopsis, 0.2 portion of feverfew, fully mix after grinding to form fine powder, continue to add 0.5 part of feed grade cellulase, mixing, add 15 parts of water furnishing pasty states again, after placing 80-85min at normal temperatures, 90 DEG C of oven dry, grind to form fine powder;
(2) composite microorganism viable bacteria powder is prepared:
By Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is hybridly prepared into compound viable bacteria powder;
(3) get 10-15 part traditional Chinese medicine powder, 0.1 part of composite microorganism viable bacteria powder, 0.1 part of nanomete talc powder fully mix and both obtained finished product.
2. the preparation method of Environment of Litopenaeus vannamei Low composite microorganism type forage additives according to claim 1, is characterized in that:
According to bacterial strain number ratio Bacillus acidi lactici: bacillus licheniformis: Corynebacterium glutamicum is: the proportions of 1:0.3:0.8 becomes compound viable bacteria powder.
3. the composite microorganism type forage additives for preparing of the preparation method of the described Environment of Litopenaeus vannamei Low composite microorganism type forage additives of claim 1-2.
4. composite microorganism type forage additives according to claim 3 is used for the application of Environment of Litopenaeus vannamei Low.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721513A (en) * | 2016-11-24 | 2017-05-31 | 江苏爱西施科技服务咨询股份有限公司 | A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof |
| CN108576484A (en) * | 2018-03-26 | 2018-09-28 | 上海亘卓生物工程有限公司 | A kind of biological agent and preparation method thereof promoting black carp inosine acid content |
| CN111944733A (en) * | 2020-09-10 | 2020-11-17 | 森井生物工程(湖州)有限公司 | Corynebacterium glutamicum and application thereof |
| CN112481164A (en) * | 2020-12-01 | 2021-03-12 | 清远海贝生物技术有限公司 | Litopenaeus vannamei growth promoting microecological preparation and use method and application thereof |
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| CN1973659A (en) * | 2006-09-22 | 2007-06-06 | 中国水产科学研究院南海水产研究所 | Prawn feed additive |
| CN102415346A (en) * | 2011-09-05 | 2012-04-18 | 澧县华润水产生物科技有限公司 | Fresh water aquaculture method for penaeus vannamei |
| CN104431415A (en) * | 2014-10-28 | 2015-03-25 | 亿利资源集团有限公司 | Active microorganism feed for ruminant animals |
| CN104472895A (en) * | 2014-10-28 | 2015-04-01 | 亿利资源集团有限公司 | Active microorganism feed for pigs |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1973659A (en) * | 2006-09-22 | 2007-06-06 | 中国水产科学研究院南海水产研究所 | Prawn feed additive |
| CN102415346A (en) * | 2011-09-05 | 2012-04-18 | 澧县华润水产生物科技有限公司 | Fresh water aquaculture method for penaeus vannamei |
| CN104431415A (en) * | 2014-10-28 | 2015-03-25 | 亿利资源集团有限公司 | Active microorganism feed for ruminant animals |
| CN104472895A (en) * | 2014-10-28 | 2015-04-01 | 亿利资源集团有限公司 | Active microorganism feed for pigs |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721513A (en) * | 2016-11-24 | 2017-05-31 | 江苏爱西施科技服务咨询股份有限公司 | A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof |
| CN108576484A (en) * | 2018-03-26 | 2018-09-28 | 上海亘卓生物工程有限公司 | A kind of biological agent and preparation method thereof promoting black carp inosine acid content |
| CN108576484B (en) * | 2018-03-26 | 2022-02-11 | 上海亘卓生物工程有限公司 | Biological agent for improving inosinic acid content of black carp and preparation method thereof |
| CN111944733A (en) * | 2020-09-10 | 2020-11-17 | 森井生物工程(湖州)有限公司 | Corynebacterium glutamicum and application thereof |
| CN112481164A (en) * | 2020-12-01 | 2021-03-12 | 清远海贝生物技术有限公司 | Litopenaeus vannamei growth promoting microecological preparation and use method and application thereof |
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Application publication date: 20160120 |