CN106721513A - A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof - Google Patents

A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof Download PDF

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CN106721513A
CN106721513A CN201611043968.8A CN201611043968A CN106721513A CN 106721513 A CN106721513 A CN 106721513A CN 201611043968 A CN201611043968 A CN 201611043968A CN 106721513 A CN106721513 A CN 106721513A
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pupa
parts
amino acids
silkworm
abundant amino
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汪屹
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Jiangsu Love Beauty Technology Advisory Services Ltd By Share Ltd
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Jiangsu Love Beauty Technology Advisory Services Ltd By Share Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof, matched by weight by following components and be made:Modified 25~42 parts of abundant amino acids from silkworm pupa, 14~26 parts of fish meal, 11~25 parts of dregs of beans, 2~12 parts of fish oil, 5~12 parts of lecithin, 7~13 parts of palm oil, 2~9 parts of soya-bean oil, 1~5 part of bacillus licheniformis, 3~8 parts of lactic acid bacteria.(1) additive comprehensive nutrition of the present invention, utilization rate be high, pollution is small;(2) additive of the present invention can improve utilization rate of the prawn to protein, improve and control fish, shrimp, the excretion of crab ammonia nitrogen, the digestive utilization ratio of raising nutrients, mitigate breeding environment pressure, reduce feed cost;(3) additive of the present invention is with strong points, noresidue, nuisanceless, it is possible to increase the immunity of prawn.

Description

A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and its preparation Method
Technical field
The invention belongs to food processing field, it is related to a kind of food additives, more particularly to one kind to contain modified silkworm chrysalis ammonia Feed for litopenaeus vannamei additive of base acid and preparation method thereof.
Background technology
During the nearly last ten years, aquaculture is worldwide developed rapidly, to human health and improvement developing country Diet structure is made that outstanding contribution.But under intensive culture pattern, the nutrient such as substantial amounts of nitrogen, phosphorus in low-quality man-made feeds Do not absorbed and be directly entered water body, as the main inducing that main pollution sources and disease occur.Water pollution it is increasingly tight Weight and intensive high-density breeding environmental pattern cause spreading unchecked for aquiculture disease.A large amount of antibiotic are used as disease-resistant growth-promoting simultaneously Agent long-term use long is even abused, and destroys the microecological balance in animal body, causes the generation of antibody-resistant bacterium, so that animal Morbidity risk rate rises, and treatment difficulty is increased, and causes vicious circle.What is more, medicine has a strong impact on and endangers in the residual of body Human health.How to improve and control the excretion of aquatic livestock ammonia nitrogen, improving the digestive utilization ratio of nutrients, deep, system Ground research builds nutrition feed in a balanced way to the demand and suitable proportionate relationship of prawn nutrition extract, improves feeding quality.Grind simultaneously System and screening noresidue, non-harmful growth promotion and disease-resistant additive are Feedstuff Enterprises, formulator and scientific worker face The problem faced.
The research purpose of prawn environment-friendly feed is:1. the environment-friendly type that comprehensive nutrition, utilization rate are high, pollution is small is developed to raise Material, improves utilization rate of the prawn to protein, improves and control fish, shrimp, the excretion of crab ammonia nitrogen, the digestibility and utilization of raising nutrients Rate, mitigates breeding environment pressure, reduces feed cost.2. with strong points, noresidue, non-harmful disease-resistant additive are developed, Improve the immunity of prawn.
Antibiotic is widely used as livestock and poultry and existing more than the 50 years history of aquatic livestock growth accelerator, is sent out in animal feeding Huge effect has been waved, the development of animal husbandry and fishery industry has been greatly promoted.But, the experimental results show, antibiotic is being raised Long-term use can cause harmful bacteria to produce drug resistance in material, and medicament residue is produced in animal body.In recent years, as the mankind give birth to The raising of the gentle health perception of running water, oneself is subject to that global range is increasingly extensive pays close attention to food-safety problem, forbids antibiotic to add Plus the cry more and more higher that agent is applied in herding and aquatic products production.A few days ago, European Union has prohibited and has been used in animal feed Antibiotic growth accelerator, ban as implementation of class is also being considered including many other countries including China.Therefore, research and Exploitation is natural, green antibiotic growth accelerator substitute, it has also become the focus class of Animal nutrition research field in global range One of topic.
The content of the invention
The purpose of the present invention is:In order to overcome the defect of prior art, a kind of comprehensive nutrition of acquisition, utilization rate are high, pollution Small environment-friendly feed additive, improves prawn to the utilization rate of protein, improves and control fish, shrimp, the excretion of crab ammonia nitrogen, carries The digestive utilization ratio of high-nutrient, mitigates breeding environment pressure, reduces feed cost, and modified silkworm is contained the invention provides one kind Feed for litopenaeus vannamei additive of pupa amino acid and preparation method thereof.
Technical scheme:A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, by following components by weight Amount number proportioning is made:Modified 25~42 parts of abundant amino acids from silkworm pupa, 14~26 parts of fish meal, 11~25 parts of dregs of beans, 2~12 parts of fish oil, 5~12 parts of lecithin, 7~13 parts of palm oil, 2~9 parts of soya-bean oil, 1~5 part of bacillus licheniformis, 3~8 parts of lactic acid bacteria.
Preferably, the feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa by following components by weight Number proportioning is made:Modified 38 parts of abundant amino acids from silkworm pupa, 22 parts of fish meal, 21 parts of dregs of beans, 9 parts of fish oil, 9 parts of lecithin, 8 parts of palm oil, 7 parts of soya-bean oil, 3 parts of bacillus licheniformis, 6 parts of lactic acid bacteria.
A kind of preparation method of the feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, comprises the steps of:
(1) silkworm chrysalis pre-treatment:Collect silkworm chrysalis, use volume fraction for 30~75% alcohol washing solution 1~3 time, then It is placed in the mixed solution of 1% formaldehyde and 3% hydrochloric acid and soaks 2~7 minutes, formaldehyde and the volume ratio of hydrochloric acid is 1 in mixed solution ~3:5~10, clear water is rinsed and puts pH in neutrality after taking-up;
(2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/5~1/2 mass is added, wears into pupa with disc mill and starch, The filtering of 30 mesh nylon mesh is crossed, then glue mill is levigate, then is filtered with 120 mesh spun silk, concentration is obtained for 26~35% pupas are starched, containing grease 12~16%;
(3) pupa slurry degreasing:The pupa that step (2) is obtained is starched -16~0 DEG C and is preserved 1~8 hour, and low-temperature centrifugation removes supernatant, To in lower sediment add 1.5~2.2 times of 3% sodium chloride solutions of volume, ultrasonically treated 22~45 minutes, 12000r/min from The heart 1~10 minute, removes supernatant, obtains pupa albumen;
(4) pupa albumen is deodorant:Deodorant 25~60 minutes using Ozonation, spray drying is made without addled egg after concentration White powder;
(5) albumen powder is modified:The odorless albumen powder obtained using the means pulverising step (4) of abrading-ball ultramicro grinding, is crushed Time is 45~85 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained;
(6) finished product:Fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and lactic acid bacteria are added In modified abundant amino acids from silkworm pupa in step (5), 2~6 DEG C are well mixed.
Preferably, step (1) silkworm chrysalis pre-treatment:Collect silkworm chrysalis, use volume fraction for 45% alcohol washing solution 3 It is secondary, then immersion 5 minutes in the mixed solution of 1% formaldehyde and 3% hydrochloric acid are placed in, formaldehyde is with the volume ratio of hydrochloric acid in mixed solution 2:9, clear water is rinsed and puts pH in neutrality after taking-up.
Preferably, step (2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/3 mass is added, ground with disc mill Pupa is starched, and crosses the filtering of 30 mesh nylon mesh, and then glue mill is levigate, then is filtered with 120 mesh spun silk, obtains concentration for 30% pupa is starched, and is contained Grease 14%.
Preferably, step (3) pupa slurry degreasing:The pupa that step (2) is obtained is starched -9 DEG C and is preserved 6 hours, and low-temperature centrifugation goes Clearly, to 1.9 times of 3% sodium chloride solutions of volume are added in lower sediment, ultrasonically treated 38 minutes, 12000r/min was centrifuged 8 points Clock, removes supernatant, obtains pupa albumen.
Preferably, step (4) pupa albumen is deodorant:Deodorant 45 minutes using Ozonation, system is spray-dried after concentration Into odorless albumen powder.
Preferably, step (5) albumen powder is modified:The odorless obtained using the means pulverising step (4) of abrading-ball ultramicro grinding Albumen powder, grinding time is 65 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained.
Preferably, step (6) finished product:By fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and Lactic acid bacteria is added in the modified abundant amino acids from silkworm pupa in step (5), and 4 DEG C are well mixed.
Beneficial effect:(1) additive comprehensive nutrition of the present invention, utilization rate be high, pollution is small;(2) it is of the present invention to add Plus agent can improve utilization rate of the prawn to protein, improve and control fish, shrimp, the excretion of crab ammonia nitrogen, improve disappearing for nutrients Change utilization rate, mitigate breeding environment pressure, reduce feed cost;(3) additive of the present invention is with strong points, noresidue, nothing Public hazards, it is possible to increase the immunity of prawn.
Specific embodiment
Embodiment 1
A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, is matched by weight by following components It is made:Modified 25 parts of abundant amino acids from silkworm pupa, 14 parts of fish meal, 11 parts of dregs of beans, 2 parts of fish oil, 5 parts of lecithin, 7 parts of palm oil, soya-bean oil 2 Part, 1 part of bacillus licheniformis, 3 parts of lactic acid bacteria.
A kind of preparation method of the feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, comprises the steps of:
(1) silkworm chrysalis pre-treatment:Silkworm chrysalis is collected, it is 30% alcohol washing solution 1 time to use volume fraction, then is placed in 1% Soaked 2 minutes in the mixed solution of formaldehyde and 3% hydrochloric acid, formaldehyde and the volume ratio of hydrochloric acid are 1 in mixed solution:5, it is clear after taking-up Water is rinsed puts pH in neutrality;
(2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/5 mass is added, wear into pupa with disc mill and starch, cross 30 Mesh nylon mesh is filtered, and then glue mill is levigate, then is filtered with 120 mesh spun silk, concentration is obtained for 26% pupa is starched, containing grease 12%;
(3) pupa slurry degreasing:The pupa that step (2) is obtained is starched -16 DEG C and is preserved 1 hour, and low-temperature centrifugation removes supernatant, to lower floor 1.5 times of 3% sodium chloride solutions of volume are added in precipitation, ultrasonically treated 22 minutes, 12000r/min was centrifuged 1 minute, removes supernatant, Obtain pupa albumen;
(4) pupa albumen is deodorant:Deodorant 25 minutes using Ozonation, spray drying is made odorless albumen after concentration Powder;
(5) albumen powder is modified:The odorless albumen powder obtained using the means pulverising step (4) of abrading-ball ultramicro grinding, is crushed Time is 45 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained;
(6) finished product:Fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and lactic acid bacteria are added In modified abundant amino acids from silkworm pupa in step (5), 2 DEG C are well mixed.
Embodiment 2
A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, is matched by weight by following components It is made:Modified 38 parts of abundant amino acids from silkworm pupa, 22 parts of fish meal, 21 parts of dregs of beans, 9 parts of fish oil, 9 parts of lecithin, 8 parts of palm oil, soya-bean oil 7 Part, 3 parts of bacillus licheniformis, 6 parts of lactic acid bacteria.
A kind of preparation method of the feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, comprises the steps of:
(1) silkworm chrysalis pre-treatment:Silkworm chrysalis is collected, it is 45% alcohol washing solution 3 times to use volume fraction, then is placed in 1% Soaked 5 minutes in the mixed solution of formaldehyde and 3% hydrochloric acid, formaldehyde and the volume ratio of hydrochloric acid are 2 in mixed solution:9, it is clear after taking-up Water is rinsed puts pH in neutrality;
(2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/3 mass is added, wear into pupa with disc mill and starch, cross 30 Mesh nylon mesh is filtered, and then glue mill is levigate, then is filtered with 120 mesh spun silk, concentration is obtained for 30% pupa is starched, containing grease 14%;
(3) pupa slurry degreasing:The pupa that step (2) is obtained is starched -9 DEG C and is preserved 6 hours, and low-temperature centrifugation removes supernatant, heavy to lower floor 1.9 times of 3% sodium chloride solutions of volume are added in shallow lake, ultrasonically treated 38 minutes, 12000r/min was centrifuged 8 minutes, removes supernatant, obtains Pupa albumen;
(4) pupa albumen is deodorant:Deodorant 45 minutes using Ozonation, spray drying is made odorless albumen after concentration Powder;
(5) albumen powder is modified:The odorless albumen powder obtained using the means pulverising step (4) of abrading-ball ultramicro grinding, is crushed Time is 65 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained;
(6) finished product:Fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and lactic acid bacteria are added In modified abundant amino acids from silkworm pupa in step (5), 4 DEG C are well mixed.
Embodiment 3
A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, is matched by weight by following components It is made:Modified 42 parts of abundant amino acids from silkworm pupa, 26 parts of fish meal, 25 parts of dregs of beans, 12 parts of fish oil, 12 parts of lecithin, 13 parts of palm oil, soya-bean oil 9 parts, 5 parts of bacillus licheniformis, 8 parts of lactic acid bacteria.
A kind of preparation method of the feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, comprises the steps of:
(1) silkworm chrysalis pre-treatment:Silkworm chrysalis is collected, it is 75% alcohol washing solution 3 times to use volume fraction, then is placed in 1% Soaked 7 minutes in the mixed solution of formaldehyde and 3% hydrochloric acid, formaldehyde and the volume ratio of hydrochloric acid are 3 in mixed solution:10, after taking-up Clear water is rinsed puts pH in neutrality;
(2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/2 mass is added, wear into pupa with disc mill and starch, cross 30 Mesh nylon mesh is filtered, and then glue mill is levigate, then is filtered with 120 mesh spun silk, concentration is obtained for 35% pupa is starched, containing grease 16%;
(3) pupa slurry degreasing:The pupa that step (2) is obtained is starched 0 DEG C and is preserved 8 hours, and low-temperature centrifugation removes supernatant, to lower sediment 2.2 times of 3% sodium chloride solutions of volume of middle addition, ultrasonically treated 45 minutes, 12000r/min was centrifuged 10 minutes, removes supernatant, obtains Pupa albumen;
(4) pupa albumen is deodorant:Deodorant 60 minutes using Ozonation, spray drying is made odorless albumen after concentration Powder;
(5) albumen powder is modified:The odorless albumen powder obtained using the means pulverising step (4) of abrading-ball ultramicro grinding, is crushed Time is 85 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained;
(6) finished product:Fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and lactic acid bacteria are added In modified abundant amino acids from silkworm pupa in step (5), 6 DEG C are well mixed.
The feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa prepared to embodiment 1~3 is examined Survey, it is as a result as shown in the table:
Embodiment 1 Embodiment 2 Embodiment 3
Survival rate/% 92.1 98.3 96.2
Specific growth rate/% 6.85 7.23 6.98
Feed efficiency 0.85 0.88 0.86
Protein efficiency ratio, PER 2.13 2.89 2.75
Energy retention rate/% 29.3 35.2 32.6

Claims (9)

1. a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa, it is characterised in that by following components by weight Amount number proportioning is made:Modified 25~42 parts of abundant amino acids from silkworm pupa, 14~26 parts of fish meal, 11~25 parts of dregs of beans, 2~12 parts of fish oil, 5~12 parts of lecithin, 7~13 parts of palm oil, 2~9 parts of soya-bean oil, 1~5 part of bacillus licheniformis, 3~8 parts of lactic acid bacteria.
2. a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 1, its feature It is to be matched by weight by following components and be made:Modified 38 parts of abundant amino acids from silkworm pupa, 22 parts of fish meal, 21 parts of dregs of beans, fish oil 9 Part, 9 parts of lecithin, 8 parts of palm oil, 7 parts of soya-bean oil, 3 parts of bacillus licheniformis, 6 parts of lactic acid bacteria.
3. the preparation method of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa described in claim 1, Characterized in that, comprising the steps of:
(1) silkworm chrysalis pre-treatment:Silkworm chrysalis is collected, it is 30~75% alcohol washing solution 1~3 time to use volume fraction, then is placed in Soaked 2~7 minutes in the mixed solution of 1% formaldehyde and 3% hydrochloric acid, formaldehyde and the volume ratio of hydrochloric acid are 1~3 in mixed solution:5 ~10, clear water is rinsed and puts pH in neutrality after taking-up;
(2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/5~1/2 mass is added, wear into pupa with disc mill and starch, cross 30 Mesh nylon mesh is filtered, and then glue mill is levigate, then is filtered with 120 mesh spun silk, obtains concentration for 26~35% pupas are starched, containing grease 12~ 16%;
(3) pupa slurry degreasing:The pupa that step (2) is obtained is starched -16~0 DEG C and is preserved 1~8 hour, and low-temperature centrifugation removes supernatant, downwards 1.5~2.2 times of 3% sodium chloride solutions of volume, ultrasonically treated 22~45 minutes, 12000r/min centrifugations 1 are added in layer precipitation ~10 minutes, supernatant is removed, obtain pupa albumen;
(4) pupa albumen is deodorant:Deodorant 25~60 minutes using Ozonation, spray drying is made odorless albumen after concentration Powder;
(5) albumen powder is modified:The odorless albumen powder obtained using the means pulverising step (4) of abrading-ball ultramicro grinding, grinding time It is 45~85 minutes, then decomposes in vinegar, modified abundant amino acids from silkworm pupa is obtained;
(6) finished product:Fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and lactic acid bacteria are added into step (5) in the modified abundant amino acids from silkworm pupa in, 2~6 DEG C are well mixed.
4. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (1) silkworm chrysalis pre-treatment:Collect silkworm chrysalis, use volume fraction for 45% alcohol washing solution 3 times, It is placed in again in the mixed solution of 1% formaldehyde and 3% hydrochloric acid and soaks 5 minutes, formaldehyde and the volume ratio of hydrochloric acid is 2 in mixed solution: 9, clear water is rinsed and puts pH in neutrality after taking-up.
5. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (2) pupa slurry is obtained:After silkworm chrysalis surface water is dried, the water of 1/3 mass is added, worn into disc mill Pupa is starched, and crosses the filtering of 30 mesh nylon mesh, and then glue mill is levigate, then is filtered with 120 mesh spun silk, obtains concentration for 30% pupa is starched, oil-containing Fat 14%.
6. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (3) pupa starches degreasing:The pupa that step (2) is obtained is starched -9 DEG C and is preserved 6 hours, and low-temperature centrifugation goes Clearly, to 1.9 times of 3% sodium chloride solutions of volume are added in lower sediment, ultrasonically treated 38 minutes, 12000r/min was centrifuged 8 points Clock, removes supernatant, obtains pupa albumen.
7. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (4) pupa albumen is deodorant:Deodorant 45 minutes using Ozonation, spray drying is made after concentration Odorless albumen powder.
8. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (5) albumen powder is modified:Using abrading-ball ultramicro grinding means pulverising step (4) obtain without addled egg White powder, grinding time is 65 minutes, is then decomposed in vinegar, and modified abundant amino acids from silkworm pupa is obtained.
9. the preparation side of a kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa according to claim 3 Method, it is characterised in that step (6) finished product:By fish meal, dregs of beans, fish oil, lecithin, palm oil, soya-bean oil, bacillus licheniformis and Lactic acid bacteria is added in the modified abundant amino acids from silkworm pupa in step (5), and 4 DEG C are well mixed.
CN201611043968.8A 2016-11-24 2016-11-24 A kind of feed for litopenaeus vannamei additive containing modified abundant amino acids from silkworm pupa and preparation method thereof Pending CN106721513A (en)

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CN114376121A (en) * 2022-02-14 2022-04-22 无锡华诺威动物保健品有限公司 Application of compound organic acid in litopenaeus vannamei breeding

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CN101828644A (en) * 2010-05-11 2010-09-15 中山大学 Feed for litopenaeus vannamei
CN105249022A (en) * 2015-11-11 2016-01-20 浙江锦天生物科技有限公司 Compound micro-ecological feed additives for litopenaeus vannamei

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114376121A (en) * 2022-02-14 2022-04-22 无锡华诺威动物保健品有限公司 Application of compound organic acid in litopenaeus vannamei breeding

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Application publication date: 20170531