CN107413821A - A kind of method that the thermophilic lysis bacterium of immobilization is used for antibiotic bacterium dregs minimizing - Google Patents
A kind of method that the thermophilic lysis bacterium of immobilization is used for antibiotic bacterium dregs minimizing Download PDFInfo
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
Landscapes
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Treatment Of Sludge (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to biodegradation technique field, specifically, is related to a kind of method that the thermophilic lysis bacterium of immobilization is used for antibiotic bacterium dregs minimizing.This method comprises the following steps：（1）The expansion culture of thermophilic lysis bacterium,（2）The immobilization of thermophilic lysis bacterium,（3）Bacteria residue minimizing is handled.The present invention is using the thermophilic lysis bacterium of immobilization as antibiotic bacterium Slag treatment bacterial strain, it makes antibiotic bacterium dregs COD clearances reach more than 80% under conditions of aerobic fermentation, also there is apparent removal effect to ammonia nitrogen and SS, minimizing to antibiotic bacterium dregs has good effect, continuous processing antibiotic bacterium dregs, without supplementing thermophilic lysis bacterium in addition, and clearance is stable.
Description
Technical field
The invention belongs to biodegradation technique field, specifically, is related to one kind and is used to resist by the thermophilic lysis bacterium of immobilization
The method of raw plain bacteria residue minimizing.
Background technology
Antibiotic plays huge effect in the survival and development of the mankind and Animal husbandry production.Up to the present,
Antibiotic is still what can not be substituted by other materials in its application field.Antibiotic bacterium dregs be antibiotics production process must
Right product.These bacteria residues because containing abundant protein, polysaccharide, amino acid, being used and drying processing etc. always before 2008
After technical finesse or feed, feed addictive are used as, or is comprehensively utilized as the raw material of fertilizer, production composite fertilizer.But
It is blue or green such as the 5mg/g of residual of the cephalosporin in bacteria residue about 2~2. because antibiotic residue is higher in antibiotic bacterium dregs
The mycin about 1~2mg/g of the residual in bacteria residue, the erythromycin about 0. 2~0.9mg/g of the residual in bacteria residue, terramycin is in bacterium
The mg/g of residual about 0. 5 ~ 2.1 in slag, these residues of residual of the tetracycline in bacteria residue about 0. 5 ~ 2mg/g are final
It will be entered by food chain in animal body and in human body.Food of the long-term intake containing aminoglycoside antibiotics residual can be led
Cause renal toxicity and ototoxicity；Food of the long-term intake containing cephalo-type penicillin antibiotics residual causes asthma, purple plague purpura；In addition
It can increase pathogenic bacteria resistance to drugs, so as to bring the difficulty of some human diseases of prevention and treatment and livestock poultry disease, most
After cause to past medical help；And they are made into Fertilizer application can cause the numerous imbalances of the microorganism ecological environment in soil, soil
Fertility declines, and accelerates stony desertification desertification；The export trade of China's livestock products is influenceed simultaneously.
After 2008, antibiotic bacterium dregs are classified as hazardous waste by country, can only be burned by dangerous waste disposal.The own warp in China
As global maximum antibiotic production of raw medicine state and exported country, oneself accounts for the 80% of global total output to raw material drug prods, wherein going out
Mouth accounts for more than the 70% of whole world bulk drug market.About 140,000 tons of antibiotic of whole nation production in 2012, its bacteria residue is more than 150
Ten thousand tons.The burning cost of bacteria residue oneself turn into antibiotic industry survival and development bottleneck.
Processing to antibiotic bacterium dregs, all seeking more appropriate treatment approach always.Many scientific research institutions and pharmacy enterprise
Industry has carried out antibiotic bacterium dregs innocent disposal and the research of different purposes.Environmental protection department also pays much attention to and encouraged antibiosis
The exploitation of the new technologies such as plain bacteria residue disposal.For example, more successfully there is 1) dregs of a decoction charing disposal；2) biomass energy cogeneration
Technology.
The content of the invention
It is an object of the invention to provide it is a kind of by the thermophilic lysis bacterium of immobilization be used for antibiotic bacterium dregs minimizing method,
Specifically include following steps：
（1）The expansion culture of thermophilic lysis bacterium
Thermophilic lysis bacterium need to be cultivated before Immobilized culture through expanding.Beef-protein medium is thermophilic molten for experiment inoculation
Culture medium used in born of the same parents bacterium, its fluid nutrient medium concrete composition composition are as follows：The g of beef extract 5.0, peptone 10.0 g, NaCl
5.0 g, sterilize under the conditions of mL, pH=7.0,121 DEG C of water 1,000 20 min.In constant-temperature table, by adjustment of rotational speed to 120r/
Min, temperature are 60 DEG C, shaken cultivation 16h.Bacterium solution is diluted with sterilized water, it is 0.7- to make its absorbance at 600 nm wavelength
0.8, so that bacterial strain is in logarithmic growth period, tested for subsequent fixedization.
（2）The immobilization of thermophilic lysis bacterium
Select 5L large beakers be used as immobilization process reaction vessel, imposed aeration device, using peristaltic pump progress nutrient solution add,
The nutrient composition of addition is consistent with beef-protein medium composition, adds nutrient solution daily, makes the organic loading of system
Maintain 0.4-0.8 kgCOD/ (m3D), and daily discharge and the reaction solution of the essentially identical volume of dosage, holding system are steady
It is fixed.Reaction solution dischargeable capacity is 4L in beaker, and it is respectively MBBR fillers to add three kinds of different fillers, and combined stuffing and suspension are spherical
Filler.Cultivated in 60 DEG C of water-baths, obtain the thermophilic lysis bacterium of immobilization.
Wherein, the moisture of the antibiotic bacterium dregs is 90.37%, and it is mould that it includes but is not limited to cephalosporin dreg, soil
Plain bacteria residue, erythromycin bacterium slag and penicillin mushroom dregs etc..
（3）Bacteria residue minimizing is handled
Antibiotic bacterium dregs will be added in the thermophilic lysis bacterium of immobilization, add antibiotic bacterium dregs once a day, bacteria residue dosage with it is anti-
It is 3 to answer system volumetric ratio:100, the volumetric loading of system is maintained 2.2-2.6kgCOD/ (m3D) between, trained at 60 DEG C
Support and add aeration, take a certain amount of reaction solution measure ammonia nitrogen and pH daily, COD is determined after centrifugal treating, SS is determined after suction filtration.
When reaction solution same day COD clearances reach 75% or higher, it may be determined that immobilization experiment is successful on the whole.
Further, step（2）The composition of middle nutrient solution is：The g of beef extract 5.0, peptone 10.0 g, NaCl 5.0 g,
The mL of water 1000, pH=7.0.
The advantages of the present invention：
The present invention provides expands the thermophilic lysis bacterium cultivated a kind of early stage through beef-protein medium, adds filler and carries out
Immobilization processing, so that antibiotic bacterium dregs minimizing, and the method for reducing the antibiotic residue in bacteria residue.The present invention uses
As antibiotic bacterium Slag treatment bacterial strain, it goes antibiotic bacterium dregs COD under conditions of aerobic fermentation for immobilization is thermophilic lysis bacterium
Except rate reaches more than 80%, also there is apparent removal effect to ammonia nitrogen and SS, the minimizing to antibiotic bacterium dregs has good effect
Fruit.
This method have the advantage that antibiotic bacterium dregs minimizing degree is high, residue removal rate is high.Immobilized thallus can be long-term
Batch operation, thalline is set more to adapt to processing environment without thalline is removed from reactor, performance plays more stable.It is fixed
The thalline of change can extend bioactivity and stability, continuous processing bacteria residue, it is not necessary to additionally supplement thermophilic lysis bacterium, more favorably
In efficiently separating for immobilized thallus and bacteria residue, the utilization ratio of immobilized microorganism is improved, so as to greatly reduce fund
Cost.This method application prospect is more wide, has obvious economic benefit, social benefit and environmental benefit.
Brief description of the drawings
Fig. 1 immobilizations processing after Thermophilic Bacteria add bacteria residue after COD value and clearance change with time；
COD value and clearance after the Thermophilic Bacteria addition bacteria residue of Fig. 2 unlockedization change with time；
Ammonia nitrogen changes with time after Fig. 3 adds penicillin mushroom dregs；
SS values change with time after Fig. 4 adds bacteria residue.
Embodiment
Embodiment 1 is used for the thermophilic lysis bacterium of immobilization and its preparation for handling antibiotic bacterium dregs
Thermophilic lysis bacterium need to be cultivated before Immobilized culture through expanding.Beef-protein medium is thermophilic molten for experiment inoculation
Culture medium used in born of the same parents bacterium, its fluid nutrient medium concrete composition composition are as follows：The g of beef extract 5.0, peptone 10.0 g, NaCl
5.0 g, sterilize under the conditions of mL, pH=7.0,121 DEG C of water 1,000 20 min.Solid medium is that 2% is added in fluid nutrient medium
Agar.In constant-temperature table, by adjustment of rotational speed to 120r/min, temperature is 60 DEG C, shaken cultivation 16h.Make it in 600 nm
Absorbance at wavelength is 0.8, is tested for subsequent fixedization.
5L large beakers are selected imposed aeration device, nutrient solution to be carried out using peristaltic pump as immobilization process reaction vessel
Add, the nutrient composition of addition is consistent with beef-protein medium composition, adds nutrient solution daily, makes the organic of system
Between load maintains 0.4-0.8 kgCOD/ (m3 d), and daily discharge and the reaction solution of dosage same volume, maintainer
System is stable.Reaction solution dischargeable capacity is 4L in beaker, adds three kinds of different fillers, respectively MBBR fillers, combined stuffing and outstanding
Floating Ball-type packing.Cultivate in 60 DEG C of water-baths, and compareed with blank group, using this index of COD clearances, to three kinds
Filler is screened, and it is optimal to filter out combined stuffing immobilization effect.
Wherein, the moisture of the antibiotic bacterium dregs is 90.37%.The antibiotic bacterium dregs include but is not limited to cephalo
Rhzomorph bacteria residue, terramycin bacteria residue, erythromycin bacterium slag and penicillin mushroom dregs etc..
The processing of the penicillin mushroom dregs of embodiment 2
（1）The change of COD clearances
The optimal immobilization Thermophilic Bacteria that testing sieve is selected is the thermophilic lysis bacterium that combined stuffing is fixed, by it with passing through again in ox
The thermophilic lysis bacterium turned out in meat extract protein culture medium while add penicillin mushroom dregs and tested.Combined in experimentation
The trend that the COD value and clearance of filler group change over time is as shown in Figure 1.
As shown in Figure 1, after reaction system adds penicillin mushroom dregs, COD value first increases, rear to reduce, and forms this phenomenon
Reason is mainly, and after adding penicillin mushroom dregs, the ectoenzyme of thermophilic lysis bacterium secretion, the microorganism in penicillin mushroom dregs is carried out
Broken born of the same parents, a large amount of intracellular organic matter discharges, make the COD value of system increase, and also to thermophilic lysis bacterium, largely propagation brings supporting for abundance
Point, thalli growth is promoted, degraded COD speed is accelerated, so that COD value constantly reduces, has reached the purpose of minimizing.
On the other hand, experiment condition is carried out under 60 DEG C of hot conditions, and the pyrolysis of high temperature will also result in COD value increase.
Complex chart 1 and Fig. 2, it can be found that in the processing procedure of continuous-stable, the thermophilic lysis bacterium after immobilization is to green grass or young crops
The clearance of mycin bacteria residue reaches more than 90%, and relatively stable, and the strain of unlockedization occurs with the increase in reaction time
Strain leakage, for COD clearances below 90%, effect is not so good as the strain after immobilization.
（2）The change of ammonia nitrogen
Ammonia nitrogen is shown in Fig. 3 with the situation of change for adding penicillin mushroom dregs processing time.
Fig. 3 is demonstrated by after adding penicillin mushroom dregs, the ammonia nitrogen change in two reactors.As can be seen that ammonia nitrogen is also in first
The trend reduced after increase, but ammonia nitrogen is slightly different with COD, ammonia nitrogen is relevant with protein and amino acid, under hot conditions,
Ammonia nitrogen is changed into NH3Volatilize, the ammonia nitrogen value in reduction system, protease or main degraded caused by certain thermophilic lysis bacterium
Approach.Ammonia nitrogen first increases, and as broken born of the same parents enter in reaction water body, breaks down proteins improve ammonia nitrogen value into amino acid, polypeptide etc., it
Afterwards with the hydrolysis of amino acid, the utilization of Thermophilic Bacteria and degrade.The diagram has embodied degradation effect of the Thermophilic Bacteria to ammonia nitrogen, Gu
Thermophilic Bacteria final process effect more unlockedization Thermophilic Bacteria effect after fixedization is more preferable, and degradation rate is higher, it may be said that from degraded ammonia
From the point of view of this efficiency of nitrogen, the Thermophilic Bacteria of immobilization has embodied preferable potentiality, completes the decrement to penicillin mushroom dregs ammonia nitrogen
Change.
（3）The change of SS clearances
SS clearances are shown in Fig. 4 with the situation of change for adding penicillin mushroom dregs processing time.
Fig. 4 is SS value changes figures, and the SS values of bacteria residue are to embody the important indicator of bacteria residue quantity, and in two reactors, SS
Value embodies downward trend always.Thermophilic lysis bacterium lysis mechanism is to answer cytase simultaneously using thermophilic microorganism energy Secretion
Effectively break the parcel of Extracellular polymers in bacteria residue zoogloea, dissolve cell membrane, improve the biodegradability of bacteria residue, and then promote bacterium
The decomposition of slag, the content of organic matter is reduced, reach preliminary decrement.This fully demonstrates thermophilic lysis bacterium to penicillin mushroom dregs
Minimizing is feasible, and the Thermophilic Bacteria of immobilization before and after the processing, from the point of view of broken born of the same parents' ability to penicillin mushroom dregs, after immobilization
Strain break born of the same parents' ability and be higher than unlockedization strain, this is with unlockedization strain as the increase in reaction time, strain loss have
Close.The strain of immobilization is higher to the SS clearances of penicillin mushroom dregs, more preferable to the reduction of bacteria residue.
By the analysis to above index, absolutely proved immobilized thallus can long-term batch operation, without by thalline
Being removed from reactor makes thalline more adapt to processing environment, and performance plays more stable.The thalline of immobilization is not relative to solid
Fixed thalline can extend bioactivity and stability, be more beneficial for efficiently separating for immobilized thallus and bacteria residue, improve solid
Surely the utilization ratio of microorganism is changed, so as to greatly reduce fund cost.This method application prospect is more wide, has obvious warp
Help benefit, social benefit and environmental benefit.
Claims (5)
- A kind of 1. method that the thermophilic lysis bacterium of immobilization is used for antibiotic bacterium dregs minimizing, it is characterised in that including following step Suddenly：（1）The expansion culture of thermophilic lysis bacteriumThermophilic lysis bacterium is inoculated into beef-protein medium, in constant-temperature table, by adjustment of rotational speed to 120r/min, temperature Spend for 60 DEG C, shaken cultivation 16h, with sterilized water dilute bacterium solution, make its absorbance at 600 nm wavelength for 0.7-0.8 it Between, tested for subsequent fixedization；（2）The Immobilized culture of thermophilic lysis bacteriumLarge beaker is selected imposed aeration device, to carry out nutrient solution as immobilization process reaction vessel using peristaltic pump and add, often Day adds nutrient solution, the organic loading of system is maintained 0.4-0.8 kgCOD/ (m3D) between, and discharge daily with adding The reaction solution of same volume is measured, keeps system stable, adds filler, cultivated in 60 DEG C of water-baths, obtain the thermophilic lysis of immobilization Bacterium；（3）Antibiotic bacterium dregs minimizing is handledAntibiotic bacterium dregs will be added in the thermophilic lysis bacterium of immobilization, add antibiotic bacterium dregs once a day, bacteria residue dosage with it is anti- It is 3 to answer system volumetric ratio:100, the volumetric loading of system is maintained 2.2-2.6kgCOD/ (m3D) between, trained at 60 DEG C Support and be aerated, extract reaction solution measure ammonia nitrogen and pH daily, COD is determined after centrifugal treating, SS is determined after suction filtration.
- 2. according to the method for claim 1, it is characterised in that step（1）Middle beef-protein medium concrete composition Composition is as follows：The g of beef extract 5.0, peptone 10.0 g, NaCl 5.0 g, sterilize under the conditions of mL, pH=7.0,121 DEG C of water 1000 20 min。
- 3. according to the method for claim 1, it is characterised in that step（2）The composition of middle nutrient solution is：The g of beef extract 5.0, Peptone 10.0 g, NaCl 5.0 g, the mL of water 1000, pH=7.0.
- 4. according to the method for claim 1, it is characterised in that step（2）In filler be MBBR fillers, combined stuffing or Suspension Ball-type packing.
- 5. according to the method for claim 1, it is characterised in that wherein, the antibiotic bacterium dregs are cephalosporin dreg, soil Mycin bacteria residue, erythromycin bacterium slag or penicillin mushroom dregs.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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