CN107299067B - Composite microecological preparation for degrading nitrite in aquaculture pond and application thereof - Google Patents
Composite microecological preparation for degrading nitrite in aquaculture pond and application thereof Download PDFInfo
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- CN107299067B CN107299067B CN201710505129.1A CN201710505129A CN107299067B CN 107299067 B CN107299067 B CN 107299067B CN 201710505129 A CN201710505129 A CN 201710505129A CN 107299067 B CN107299067 B CN 107299067B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/341—Consortia of bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/166—Nitrites
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
Abstract
The invention discloses a composite microecological preparation for degrading nitrite in an aquaculture pond and application thereof. The invention discloses a microbial preparation for degrading nitrite and inhibiting pathogenic bacteria in a shrimp pond, in particular to a microbial preparation for degrading nitrite and a microbial ecological preparation, belonging to the technical field of cultivation. The invention selects pseudomonas, bacillus amyloliquefaciens and bacillus subtilis with denitrification function and takes bran, zeolite powder and corn starch as auxiliary materials to prepare the micro-ecological preparation with mixed powder particles. On one hand, the invention introduces micromolecular carbon source into water body to enhance the metabolic activity of the original denitrifying bacteria and the original heterotrophic nitrifying bacteria in the pond; on the other hand, the adsorbents with different specific gravities enable the microorganism bacteria to simultaneously play a role in the bottom of the tank and the water body, and reduce the release of ammonia nitrogen and nitrite in the bottom of the tank; thirdly, the use of the bacillus amyloliquefaciens can improve the water quality and inhibit the number of pathogenic bacteria such as vibrio and the like so as to reduce the occurrence of fish diseases.
Description
Technical Field
The invention relates to a microbial preparation for degrading nitrite and inhibiting pathogenic bacteria in a shrimp pond, in particular to a microbial ecological preparation for degrading nitrite, belonging to the technical field of cultivation.
Background
At present, with the continuous development of aquaculture, the scale of high-density aquaculture is continuously enlarged, the water body pollution is increasingly serious, ammonia nitrogen and nitrite are too high, the direct harm effect on cultured animals is achieved, and meanwhile, the deteriorated water environment enables pathogenic bacteria to propagate in a large quantity, so that the disease outbreak is caused.
Microbial agents are widely accepted for reducing ammonia nitrogen and nitrite in water. Many complex microbial preparations have appeared, but the using effect is not stable. Compared with nitrosobacteria, autotrophic nitrifying bacteria have slow propagation speed and are not suitable for the culture water body with serious eutrophication of the water body. Particularly, after the water quality is deteriorated, the dissolved oxygen in the pond is at a low level for a long time, and the degradation effect of the added nitrifying bacteria on ammonia nitrogen and nitrite is limited, so that the problem of unstable use effect often occurs.
The photosynthetic bacteria can reduce the ammonia nitrogen level in the water body, but have poor effect on reducing the nitrite in the water body; the bacillus subtilis can reduce the levels of ammonia nitrogen and nitrite in a water body, but has limited effect; autotrophic nitrifying bacteria and nitrosobacteria are not suitable for the culture water body with high eutrophication. Denitrifying bacteria have been widely used in denitrification reactions in sewage treatment, and in a biological denitrification system, denitrifying bacteria may use a carbon source as an electron donor and nitrate and nitrite as electron acceptors to reduce nitrate and nitrite into nitrogen while achieving the effect of removing organic matters and nitrogen pollutants. More strains (such as CGMCC No.7330, CGMCC No.7650 and GCMCC No.8539) are isolated at present, and even heterotrophic nitrification-anaerobic denitrification strains (CCTCC 02M207075) are included.
According to the follow-up investigation of the culture water quality, a considerable amount of denitrifying bacteria exist in water bodies and bottom sediment of the black fish pond and the crab pond, the bacterial strains have good degradation effect on nitrite in a culture medium, but high ammonia nitrogen and nitrite accumulation still occur in the culture water body, which indicates that the action process is blocked.
In high-density culture water, the most main source of ammonia nitrogen and nitrite is protein decomposition in feed. The relatively high protein content of aquatic animals determines the high protein level of their feed relative to livestock breeding, while the protein content of the feed varies widely among different breeding subjects. The shrimp and crab feed and the feed of some carnivorous fishes have higher protein content, and the research shows that the accumulation of ammonia nitrogen and nitrous acid is easier to occur in shrimp and crab ponds, and the COD/TN in a water body is about 6: 1, the C/N value is clearly lower. The C/N value in the sewage treatment reaches at least 20, so that the good nitrogen removal rate is achieved, and the lower C/N value of the aquaculture water is not beneficial to the denitrification. Therefore, we speculate that the key factor of the accumulation of ammonia nitrogen and nitrite in the water body is that the carbon source in the sediment and the water body is insufficient, so that the metabolism of nitrogen by denitrifying bacteria and heterotrophic nitrifying bacteria is blocked, and the accumulation of ammonia nitrogen and nitrite is caused.
The carbon source is an essential substance for the denitrification process. The commonly used additional carbon source in sewage treatment mainly comprises: methanol, ethanol, sodium acetate, and the like. Sodium acetate is a simple compound with low molecular weight, is easy to be utilized by microorganisms, and has relatively simple degradation process. The investigation shows that when sodium acetate is used as an external carbon source for denitrification, the COD value of the effluent can be maintained at a lower level even if the sodium acetate is excessively added. In a denitrification system, sodium acetate is used as a carbon source, so that the effect speed is high, denitrification is preferentially carried out, and thallus reproduction is not carried out, and the using effect of the microbial inoculum can be promoted.
At present, beneficial microorganisms are increasingly accepted and paid attention to application in water quality purification in China, but research is only in the initial stage, and microbial ecological agents mainly focus on simple mixing of functional microorganisms, and influence of water quality environment on function exertion of the functional microorganisms is ignored. Particularly, in the aspect of degrading ammonia nitrogen and nitrite in water, a plurality of strains for degrading ammonia nitrogen and nitrite are separated at present, but the using effect is unstable, and the main reason is that the influence of the using environment on the metabolism of microorganisms is ignored. In addition, the microorganisms can reduce the occurrence of aquaculture diseases, but the microorganisms are only limited to the simple description of the action effect, the action mechanism is only rarely known, and the invention can clearly control the diseases of aquatic animals in the actual production by monitoring the vibrio number and the disease occurrence condition of the aquaculture water.
Disclosure of Invention
The invention aims to solve the technical problem of providing a composite microecological preparation for degrading nitrite in an aquaculture pond, which has the function of inhibiting vibrio so as to solve the problem that nitrite accumulation and bacterial diseases occur successively. Aiming at the problem, the invention defines that the microbial preparation is used in an aquaculture pond into which high-protein feed such as black carp, shrimp and the like is put.
In order to solve the technical problems, the invention selects micromolecular carbon sources such as sodium acetate or sodium propionate or sodium citrate as carbon sources to promote the denitrification of the water body so as to reduce the nitrite level of the water body; pseudomonas, bacillus amyloliquefaciens and bacillus subtilis with denitrification function are selected, and bran, zeolite powder and corn starch are taken as auxiliary materials to prepare the micro-ecological preparation with mixed powder particles.
Wherein the Pseudomonas is Pseudomonas fredrickbergii (Pseudomonas frederiksbergensis).
Wherein the auxiliary material is zeolite powder which is used for adsorbing microorganisms and making the microorganisms sink to the water bottom.
Wherein, the auxiliary material bran is used for adsorbing microorganisms to enable the microorganisms to be suspended in water for a long time.
Wherein, the auxiliary material sodium acetate or sodium diacetate or sodium propionate or sodium citrate is used as a micromolecular carbon source to promote the degradation rate of denitrifying bacteria to ammonia nitrogen and nitrite.
The starch is used as a dispersing agent to keep the microbial inoculum dry; on the other hand, the starch is very soluble in water and can be used as a carbon source or a supplement of a small molecular carbon source.
The microecological preparation is in a granular form, and is prepared from Fredrickettella pseudomonad powder, bacillus subtilis powder and auxiliary materials of sodium acetate, sodium propionate or sodium citrate in a mass ratio of 2:1: and 2, granulating.
The microecological preparation is in powder form, is prepared by uniformly mixing Fredrickettger pseudomonas powder, bacillus subtilis powder and bacillus amyloliquefaciens powder with auxiliary materials of sodium acetate or sodium propionate or sodium citrate and corn starch in a mass ratio of 2:1:1:6: 2.
the microecological preparation comprises the following components in parts by mass: 3, mixing.
On one hand, the invention introduces micromolecular carbon source into water body to enhance the metabolic activity of the original denitrifying bacteria and the original heterotrophic nitrifying bacteria in the pond; on the other hand, the adsorbents with different specific gravities enable the microorganism bacteria to simultaneously play a role in the bottom of the tank and the water body, and reduce the release of ammonia nitrogen and nitrite in the bottom of the tank; thirdly, the use of the bacillus amyloliquefaciens can improve the water quality and inhibit the number of pathogenic bacteria such as vibrio and the like so as to reduce the occurrence of fish diseases.
After the microbial agent is used in river and crab ponds, the effect of degrading ammonia nitrogen and nitrite is obvious.
The invention is realized by the following technical scheme.
A microecological preparation comprises Pseudomonas fredrickbergii, Bacillus amyloliquefaciens, Bacillus subtilis and adjuvants.
The microecological preparation is in a granular form, and is prepared from Fredrickettella pseudomonad powder, bacillus subtilis powder and auxiliary materials of sodium acetate or sodium propionate or sodium citrate in a mass ratio of 2:1: and 2, granulating.
The microecological preparation is in powder form, is prepared by uniformly mixing Fredrickettger pseudomonas powder, bacillus subtilis powder and bacillus amyloliquefaciens powder with auxiliary materials of sodium acetate or sodium propionate or sodium citrate and corn starch in a mass ratio of 2:1:1:6: 2.
the microecological preparation comprises the following components in parts by mass: 3, mixing.
The microecological preparation is applied to the field of aquaculture.
The microecological preparation is used for reducing the contents of nitrite and ammonia nitrogen in water.
The microecological preparation is used for preventing and treating bacterial diseases of aquaculture animals.
Bacillus subtilis (Bacillus subtilis) is preserved in China Center for Type Culture Collection (CCTCC) AB 90008.
Pseudomonas fredrickbergii (Pseudomonas frederiksbergensis) is preserved in China center for type culture Collection, and has a preservation number of CCTCC AB 212328.
The Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSSW-LA is preserved in China center for type culture collection with the preservation number of CCTCC M2015602.
Detailed Description
Example 1: preparation of pseudomonas fredrickbergii powder
(1) Seed tank culture: preparing an aseptic triangular flask with glass beads inside, scraping and washing mature bacterial sludge on the inclined plane of the eggplant bottle by using aseptic water, filling the mature bacterial sludge into the triangular flask, oscillating and dispersing the bacterial sludge to obtain uniform bacterial suspension, inoculating the uniform bacterial suspension into a 100L seeding tank according to the inoculation amount of 2-10% of the volume ratio, wherein the filling coefficient of the seeding tank is 60-70% (v/v), namely 60-70L of 100L seed tank culture medium.
The seeding tank culture conditions comprise stirring rotation speed of 200r/min, ventilation amount of 1-3m3/h, culture temperature of 25 deg.C, and culture time of 20-48 h.
Seeding tank culture medium: 6g/L glucose, 12g/L yeast powder, 3.5g/L MgSO4.7H2O, 42.5g/L KH2PO42.5g/L CaCl21.5g/L, pH7.0-7.2.
(2) And (3) fermentation culture, namely inoculating the seed culture solution into a 1T fermentation tank according to the inoculation amount of 5.0-10.0% of the volume ratio, wherein the filling coefficient of the fermentation tank is 60-70% (v/v), the temperature is 25 ℃, the stirring speed is 200r/min, the ventilation quantity is 1-3m3/h, the culture is 48-72 h, and the quantity of the fermentation liquid thalli is more than or equal to 8 × 108CFU/mL after the fermentation is finished.
Fermentation tank culture medium: 10g/L glucose, 10g/L yeast powder, 3.5g/L MgSO4.7H2O, 3.5g/L KH2PO42.5g/L CaCl21.5g/L, pH7.0-7.2.
(3) Preparing microcapsules:
a. preparation of embedding Agents
And (3) centrifuging the fermentation liquor obtained in the step (2) to collect wet thalli, and adding sterile water to prepare a bacterial suspension, wherein the concentration of the thalli is more than or equal to 1.0 × 1010 CFU/mL.
The embedding agent is prepared by fully mixing 0-2.0 percent of sodium alginate, 0.2-0.5 percent of sodium acetate, sodium propionate or sodium citrate, 0.5-3.0 percent of gelatin and 0.1-1.0 percent of starch, wherein the used solvent is deionized water.
b. Preparation of the crosslinking agent
Preparing calcium chloride deionized water solution with the weight percentage concentration of 2.0-3.0%, and sterilizing for later use;
c. preparation of microcapsule particles
Preparing a mixed glue solution of a wall material and a bacterial solution according to a volume ratio of the bacterial solution to an embedding medium of 1: 3-5, fully stirring uniformly, spraying the mixed glue solution into a magnetically stirred calcium chloride deionized water solution with the concentration of 2.0-3.0% to form microcapsules with the diameter of 2-3 mm, standing and curing for 12-24 h to obtain pseudomonas fredrickbergii microcapsule particles, wherein 200-300 microcapsules with the diameter of 2-3 mm can be prepared from each ml of bacterial solution;
(4) and (3) determining the number of viable bacteria in the microcapsule particles: adding microcapsule particles prepared from the 1ml of the bacterial liquid into 9ml of 0.2mol/L sodium citrate solution, oscillating and dissolving the microcapsule particles, and counting viable bacteria by adopting a flat plate counting method.
(5) And (3) preparing powder, namely performing vacuum freeze drying on the microcapsule particles obtained in the step (3), crushing the dried microcapsule particles by using a crusher, sieving, and preparing the powder according to the method, wherein the viable count of the pseudomonas fradori kesburg is more than or equal to 5.0 × 108 CFU/mL.
Example 2: preparation of bacillus subtilis powder
(1) Activating strains: aseptically starting the freeze-dried preserved strain of the bacillus subtilis, streaking and inoculating the strain to a nutrient agar test tube inclined plane, culturing for 24-48h at 30-37 ℃, streaking and transferring to a nutrient agar eggplant bottle inclined plane, and culturing for 24-48h at 30-37 ℃; microscopic examination shows that when over 90% of the thallus forms spores, the thallus is mature.
The composition of the slant culture medium is measured in g/L: 10 parts of peptone, 3 parts of beef extract, 5 parts of NaCl and 15-20 parts of agar, and the peptone, the beef extract and the agar are prepared by distilled water with constant volume and pH of 7.0-7.2;
seed tank culture: preparing an aseptic triangular flask filled with glass beads, scraping and washing mature bacterial sludge on the inclined plane of the eggplant bottle by using aseptic water, filling the eggplant bottle into the aseptic flask, and oscillating and dispersing the bacterial sludge to obtain uniform bacterial suspension. Heating the bacterial suspension in 80 ℃ water bath for 10 minutes, inoculating the bacterial suspension into a 100L seed tank according to the inoculation amount of 2-10% of the volume ratio, wherein the filling coefficient of the seed tank is 60-70% (v/v), namely 60-70L of 100L seed tank culture medium.
Fermentation culture: inoculating the seed culture solution into a 1T fermentation tank by an inoculation amount of 5.0-10.0% in volume ratio, wherein the filling coefficient of the fermentation tank is 60-70% (v/v), the temperature is 37 ℃, the stirring speed is 200r/min, the ventilation amount is 1-3m3/h, and the culture is carried out for 15-30 h.
The composition of the culture medium is calculated by g/L: 1.0-5.0 of starch, 1.0-5.0 of bran, 1.0-2.0 of NaCl, 2.0-10.0 of yeast extract, 1.1-0.5 of KH2PO40 and 7.0-7.2 of pH.
Preparing a bacterial powder preparation:
and (3) continuously centrifuging the fermentation liquor obtained in the step (3) to collect wet thalli, preparing starch with the weight percentage concentration of 1.0-3.0% and gelatin with the weight percentage concentration of 1.0-3.0% according to the mass of the centrifuged thalli, fully mixing, and carrying out spray drying, wherein the air inlet temperature is 160-180 ℃, the air outlet temperature is 60-80 ℃, so as to obtain the bacillus subtilis powder, and the bacterial concentration is more than or equal to 5.0 × 109 CFU/g.
Example 3: preparation of bacillus amyloliquefaciens powder
(1) Activating strains: aseptically starting a freeze-dried preservation strain of the bacillus amyloliquefaciens JSSW-LA, streaking and inoculating the strain to a nutrient agar test tube inclined plane, culturing for 24-48h at 30-37 ℃, streaking and transferring to a nutrient agar eggplant bottle inclined plane, and culturing for 24-48h at 30-37 ℃; microscopic examination shows that when over 90% of the thallus forms spores, the thallus is mature.
The composition of the slant culture medium is measured in g/L: 10 parts of peptone, 3 parts of beef extract, 5 parts of NaCl and 15-20 parts of agar, and the peptone, the beef extract and the agar are prepared by distilled water with constant volume and pH of 7.0-7.2;
(2) seed tank culture: preparing an aseptic triangular flask filled with glass beads, scraping and washing mature bacterial sludge on the inclined plane of the eggplant bottle by using aseptic water, filling the eggplant bottle into the aseptic flask, and oscillating and dispersing the bacterial sludge to obtain uniform bacterial suspension. Heating the bacterial suspension in 80 ℃ water bath for 10 minutes, inoculating the bacterial suspension into a 100L seed tank according to the inoculation amount of 2-10% of the volume ratio, wherein the filling coefficient of the seed tank is 60-70% (v/v), namely 60-70L of 100L seed tank culture medium.
(3) Culturing in a fermentation tank: inoculating the seed culture solution into a 2T fermentation tank in an inoculation amount of 5.0-10.0% by volume ratio, wherein the filling coefficient of the fermentation tank is 60-70% (v/v), the temperature is 37 ℃, the stirring speed is 200r/min, the ventilation amount is 1-3m3/h, and the culture is carried out for 15-30 h.
The composition of the culture medium is calculated by g/L: 5-30 parts of bran, 1.0-3.0 parts of yeast extract and 2.0-5.0 parts of NaCl, and the components are prepared by distilled water with constant volume and pH of 7.0;
(4) preparation of bacterial powder preparation the bacterial powder preparation obtained by the same method as in example 2 has a bacterial concentration of 5.0 × 109CFU/g or more.
Example 4: preparation of composite microecological preparation
Particle type: mixing Friedel-crafts pseudomonas powder and bacillus subtilis powder with auxiliary materials of sodium acetate or sodium propionate or sodium citrate according to the weight ratio of 2:1:2, and granulating.
Wherein, the granulation process comprises the following steps: granulating by using a disc granulator: spraying water intermittently during granulation, air drying at a temperature of not more than 50 deg.C until the water content is below 20%, and sieving to obtain granule with particle diameter of 1-4 mm.
Powder preparation: the preparation method comprises the steps of uniformly mixing Fradricksburg pseudomonad powder, bacillus subtilis powder and bacillus amyloliquefaciens powder with auxiliary materials of sodium acetate or sodium propionate or sodium citrate and corn starch in a weight ratio of 2:1:1:6: 2.
The granular microecological preparation and the powder preparation are uniformly mixed according to the mass ratio of 1: 3.
Example 5: laboratory experiment
3 experimental ponds, the volume of each experimental pond is one, water with high ammonia nitrogen and nitrite in the pond is taken, in three ponds, in order to simulate the dynamics of the aquaculture pond, 10 small carps are respectively put in each pond, the ammonia nitrogen and nitrite in the water body are measured after three days as initial values, 2ppm of microbial inoculum is used, and the ammonia nitrogen and the nitrite in the water body are measured every day after the microbial inoculum is used once.
Water quality ammonia nitrogen data sheet
| Ammonia nitrogen (mg/L) | Initial value | The next day | The third day | The fourth day | The seventh day |
| 1 | 7.19 | 6.07 | 5.04 | 4.21 | 1.71 |
| 2 | 4.38 | 4.06 | 3.65 | 2.38 | 1.13 |
| 3 | 5.28 | 4.67 | 3.52 | 2.18 | 1.25 |
Water nitrite data sheet
Example 6: crab pool experiment
3 adjacent river crab ponds, 50 mu of each pond and the bottom of 4 months begin to accumulate nitrite, the situation is not good when the pond is 5 months, No. 5 month 21 begins to use the microecological preparation, 500 g/mu is used for the first time, and 200 g/mu is used for the next week. And the ammonia nitrogen and nitrite levels of the water body are measured before use. And (3) tracking and measuring ammonia nitrogen and nitrite in the water body after use, and taking the average value (1 st detection in 5 days in 6 months, 2 nd detection in 25 days in 6 months, 3 rd detection in 5 days in 7 months, and 3 rd detection in 25 days in 7 months).
Water quality ammonia nitrogen data sheet
| Ammonia nitrogen (mg/L) | Initial value | 1 | 2 | 3 | 4 |
| What is needed | 0.73 | 0.27 | 0.54 | 0.44 | 0.23 |
| DC5 | 0.52 | 0.44 | 0.34 | 0.24 | 0.13 |
| Qiu (a child) | 0.37 | 0.54 | 0.2 | 0.1 | 0.4 |
Water nitrite data sheet
After the microbial preparation is used, the disease condition is as follows:
after the microbial agent is used in the river and crab pond, the effect of degrading ammonia nitrogen and nitrite is obvious. The microecological preparation is used by three crab ponds at regular intervals, the microecological preparation is used once a week according to 200 g/mu, and after the microecological preparation is used, the large-area death of the crab ponds does not occur. Particularly, under the condition of frequent cloudy rainfall in 6 and 7 months, the water quality of the three crab ponds is relatively stable, large-area death does not occur, and the death amount of the adjacent crab ponds is relatively large. In 6-7 months, twice-monthly sampling monitoring shows that the vibrio number of the crab pond using the microbial agent is averagely 23CFU/mL, while the crab pond without the microbial agent is averagely 130 CFU/mL.
Finally, it should be noted that: although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention and it is intended to cover in the claims the invention as defined in the appended claims.
Claims (2)
1. A compound microecological preparation is characterized in that the formula of the preparation comprises pseudomonas stutzeri, bacillus amyloliquefaciens, bacillus subtilis and auxiliary materials; uniformly mixing pseudomonas sturgeon powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, and auxiliary materials of sodium acetate or sodium propionate or sodium citrate and corn starch in a mass ratio of 2:1:1:6: 2; the Pseudomonas stutzeri is Pseudomonas fredrickbergii (Pseudomonas frederiksbergensis), and has a preservation number of CCTCC AB 212328; the preservation number of the bacillus subtilis is CCTCC AB 90008; the Bacillus amyloliquefaciens is Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) JSSW-LA with the preservation number of CCTCC M2015602; the sodium acetate is micromolecular carbon source sodium acetate or sodium diacetate.
2. Use of the composite microecological formulation according to claim 1 for the preparation of a product for reducing the nitrite, ammonia nitrogen content of a water body and inhibiting bacterial diseases in aquaculture animals.
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| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| CN103395890A (en) * | 2013-07-19 | 2013-11-20 | 上海创博生态工程有限公司 | Microbial preparation for improving freshwater aquaculture water body and preparation method thereof |
| WO2014086856A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a biopesticide |
| CN104045164A (en) * | 2013-03-15 | 2014-09-17 | 上海微明生物科技有限公司 | Microbial preparation for improvement of freshwater aquaculture water body |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703359A (en) * | 2012-06-12 | 2012-10-03 | 上海亘卓生物工程有限公司 | Microorganism preparation for improving cultivation water and preparation method thereof |
| WO2014086856A1 (en) * | 2012-12-04 | 2014-06-12 | Basf Agro B.V., Arnhem (Nl) | Compositions comprising a quillay extract and a biopesticide |
| CN104045164A (en) * | 2013-03-15 | 2014-09-17 | 上海微明生物科技有限公司 | Microbial preparation for improvement of freshwater aquaculture water body |
| CN103395890A (en) * | 2013-07-19 | 2013-11-20 | 上海创博生态工程有限公司 | Microbial preparation for improving freshwater aquaculture water body and preparation method thereof |
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