CN114504065A - Method for producing natural bait low-temperature composite algae for aquatic animals - Google Patents
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Abstract
The invention discloses a method for producing natural bait low-temperature compound algae for aquatic animals, which comprises the following steps of S1, weighing raw materials, S2, crushing treatment: firstly, respectively weighing required parts by weight of composite algae, composite amino acid stock solution, composite enzyme, boric acid, fermentation composite bacteria, zinc sulfate heptahydrate, zeolite powder, sodium dodecyl sulfate, shell powder, peregal and water by using a batching device, storing the weighed components by using a storage device for later use, and performing S3 enzymolysis and fermentation treatment, S4 primary mixing, S5 secondary mixing, wherein the invention relates to the technical field of aquatic feed production. The method for producing the natural bait low-temperature composite algae for the aquatic animals can realize that the bait can supplement richer amino acid for the aquatic animals by adding the composite amino acid into the bait, so that the composite algae bait is richer in nutrition, and can realize the quick and sufficient enzymolysis and fermentation of the composite algae bait by adopting the cooperation of the composite enzyme and the composite bacteria.
Description
Technical Field
The invention relates to the technical field of aquatic feed production, in particular to a method for producing natural feed low-temperature compound algae for aquatic animals.
Background
Aquatic products are the general term for animals or algae produced in oceans, rivers and lakes, and the general term for related services or processing industries, such as aquatic product processing, aquatic product medicine, aquatic product export trade, ornamental aquatic products and the like. With the development of aquatic science and technology, the compound feed has the advantages of comprehensive and reasonable nutritional ingredients, good palatability, less loss and waste, convenient feeding, high feed reward and the like, and is gradually recognized and accepted by vast breeders, natural bait is fishing bait made of animals or plants, algae is a kind of eukaryote in the protist kingdom, is mainly aquatic, has no vascular bundle and can perform photosynthesis. The size of the single cell flagellates with the size of 1 micron and the large brown algae with the size of 60 meters are different. Some authoritative experts continue to group algae into plants or plant-like organisms, but algae do not have true roots, stems, leaves, nor vascular bundles. This is the same as for bryophytes, algae can be composed of one or a few cells, and many cells can aggregate into a tissue-like architecture. The filaments may or may not be branched, some of the algae being unicellular dinoflagellates, others aggregating into populations. The pinus genus of green algae is formed by interweaving and winding numerous branch filaments, and the filament shapes and functions are different at different positions. Although the algae is mainly aquatic, the algae is ubiquitous and distributed from temperate forests to polar cannervous sources, and the algae is natural bait for aquatic animals, so that the algae can be produced and processed into the natural bait.
The existing composite algae bait is not rich enough in nutrition and single in nutritional ingredients, and can not supplement more rich amino acids for aquatic animals by adding composite amino acids into the bait, meanwhile, the existing composite algae bait is insufficient in production process, enzymolysis and fermentation are not sufficient, the aim of quickly and sufficiently transforming and absorbing active ingredients in composite algae by adding boric acid, zinc sulfate heptahydrate and sodium dodecyl sulfate can not be fulfilled by adopting the complex enzyme and the composite bacteria in a matched mode, and accordingly breeding of the produced animals is very unfavorable.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a method for producing natural bait low-temperature composite algae for aquatic animals, which solves the problems that the conventional composite algae bait is not rich enough in nutrition and single in nutritional ingredients, the bait can not supplement more abundant amino acids for the aquatic animals by adding composite amino acids into the bait, meanwhile, the conventional composite algae bait is insufficient in enzymolysis and fermentation in the production process, the rapid and sufficient enzymolysis and fermentation of the composite algae bait can not be realized by adopting the complex enzyme and the composite bacteria in a matched manner, and the purpose of rapidly converting and absorbing active ingredients in the composite algae by adding boric acid, zinc sulfate heptahydrate and sodium dodecyl sulfate can not be achieved.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a method for producing natural bait low-temperature composite algae for aquatic animals specifically comprises the following steps:
s1, weighing raw materials: firstly, respectively weighing 10-20 parts of compound algae, 10-20 parts of compound amino acid stock solution, 5-10 parts of compound enzyme, 5-10 parts of boric acid, 5-10 parts of fermentation compound bacteria, 5-10 parts of zinc sulfate heptahydrate, 3-5 parts of zeolite powder, 3-5 parts of sodium dodecyl sulfate, 1-3 parts of shell powder, 1-3 parts of peregal and 1-3 parts of water by using a batching device, and storing the weighed components by using a storage device for later use;
s2, crushing treatment: pouring the fresh composite algae weighed in the step S1 into cleaning equipment, repeatedly washing for 2-3 times by using clear water, filtering out impurities, dehydrating, freezing at-15 ℃ to-25 ℃ for 3-5h, taking out, and fully crushing the frozen composite algae by using crushing equipment, wherein the mass ratio of the feed liquid is 1: 0.5-0.7, adding clear water, and stirring by a stirring device at a rotating speed of 300-500r/min to prepare homogenate;
s3, enzymolysis and fermentation treatment: adding the complex enzyme weighed in the step S1 into the homogenate obtained in the step S2, starting a stirring device to carry out enzymolysis for 10-13h at the rotation speed of 200-300r/min, separating enzymolysis liquid and residues through a filtering device after the enzymolysis is finished, mixing the fermentation complex bacteria weighed in the step S1 with the residues through the mixing stirring device, and fermenting for 15-22h according to fermentation conditions;
s4, primary mixing: mixing the enzymolysis liquid obtained in the step S3 with the fermentation product obtained in the step S3, respectively adding the compound amino acid stock solution, the zeolite powder and the shell powder, and uniformly stirring at the rotating speed of 600-700r/min by using stirring equipment;
s5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture primarily mixed in the step S4, continuously mixing and stirring for 1-2h at the rotating speed of 800-.
Preferably, the composite algae in step S1 is any combination of two or more of brown algae, red algae, diatom, green algae, gulfweed, or fucus.
Preferably, the composite amino acid stock solution in step S1 is any combination of two or more of lysine, methionine, threonine, aspartic acid, serine, glutamic acid, alanine, cystine, or tyrosine.
Preferably, the complex enzyme in step S1 is any combination of two or more of glucanase, cellobiohydrolase, 1, 4-beta-mannuronic acid lyase, bromelain or glucoamylase.
Preferably, the composite fermenting bacteria in step S1 is any combination of two or more of aspergillus niger, bacillus licheniformis, neurospora crassa, bacillus coagulans, bacillus subtilis, bacillus natto and bacillus megaterium.
Preferably, the crushing device in the step S2 is a tissue crusher with model number ZOLLO-UP 250.
Preferably, in the step S1, the washed and impurity-removed composite algae is dehydrated to a water content of 20-30 wt%.
Preferably, the fermentation conditions in step S3 are: the fermentation temperature is 25-45 deg.C, and the fermentation pH is 5.3-7.6.
(III) advantageous effects
The invention provides a method for producing natural bait low-temperature compound algae for aquatic animals. Compared with the prior art, the method has the following beneficial effects: the method for producing the natural bait low-temperature compound algae for the aquatic animals comprises the following steps: s1, weighing raw materials, S2, crushing, S3, enzymolysis and fermentation, S4, primary mixing, S5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture mixed at the time in the step S4, continuously mixing and stirring for 1-2h at the rotating speed of 800-, then transferring the mixture to drying and granulating equipment for drying and granulating so as to prepare the composite algae bait, so that the bait can supplement more abundant amino acid to aquatic animals by adding the composite amino acid into the bait, the composite algae bait is more nutritious, the compound enzyme and the compound bacteria are matched for use, so that the compound algae bait is quickly and fully subjected to enzymolysis and fermentation, and the aims of quickly converting and absorbing effective ingredients in the compound algae by adding boric acid, zinc sulfate heptahydrate and sodium dodecyl sulfate are well fulfilled, so that the compound algae bait is very beneficial to cultivation of production animals.
Drawings
FIG. 1 is a flow chart of the production method of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1, the embodiment of the present invention provides three technical solutions: a method for producing natural bait low-temperature composite algae for aquatic animals specifically comprises the following embodiments:
example 1
S1, weighing raw materials: firstly, respectively weighing 15 parts of compound algae, 15 parts of compound amino acid stock solution, 7 parts of compound enzyme, 7 parts of boric acid, 7 parts of fermentation compound bacteria, 7 parts of zinc sulfate heptahydrate, 4 parts of zeolite powder, 4 parts of sodium dodecyl sulfate, 2 parts of shell powder, 2 parts of peregal and 2 parts of water by using a batching device, storing the weighed components by using a storage device for later use, wherein the compound algae is a composition of brown algae, red algae, diatom, green algae, gulfweed and Fucus vesiculosus, the compound amino acid stock solution is a composition of lysine, methionine, threonine, aspartic acid, serine, glutamic acid, alanine, cystine and tyrosine, the compound enzyme is a composition of glucanase, cellobiohydrolase, 1, 4-beta-mannuronic acid lyase, bromelain and glucoamylase, and the fermentation compound bacteria are compositions of Aspergillus niger, bacillus, The composite algae is prepared by the steps of cleaning and removing impurities of a composition of the rough vein bacillus, the bacillus coagulans, the bacillus subtilis, the bacillus natto and the bacillus megaterium until the water content is 25 wt%;
s2, crushing treatment: pouring the fresh composite algae weighed in the step S1 into cleaning equipment, repeatedly washing for 2 times by using clear water, filtering impurities, dehydrating, freezing at-20 ℃ for 4 hours, taking out, and fully crushing the frozen composite algae by using crushing equipment, wherein the mass ratio of the feed liquid is 1: 0.6 adding clear water, and then stirring at a rotation speed of 400r/min by a stirring device to prepare homogenate, wherein the crushing device adopts a tissue crusher with a model number of ZOLLO-UP 250;
s3, enzymolysis and fermentation treatment: adding the compound enzyme weighed in the step S1 into the homogenate obtained in the step S2, starting a stirring device to carry out enzymolysis for 12 hours at the rotating speed of 250r/min, separating enzymolysis liquid and residues through a filtering device after the enzymolysis is finished, mixing the fermentation compound bacteria weighed in the step S1 with the residues through a mixing stirring device, and fermenting for 19 hours according to fermentation conditions, wherein the fermentation conditions are as follows: the fermentation temperature is 35 ℃, and the fermentation pH is 6.5;
s4, primary mixing: mixing the enzymolysis liquid obtained in the step S3 with the fermentation product obtained in the step S3, then respectively adding the compound amino acid stock solution, the zeolite powder and the shell powder, and uniformly stirring at the rotating speed of 650r/min by using stirring equipment;
s5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture primarily mixed in the step S4, continuously mixing and stirring for 1.5 hours at the rotating speed of 900r/min, and then transferring to a drying and granulating device for drying and granulating to obtain the composite algae bait.
Example 2
S1, weighing raw materials: firstly, respectively weighing 10 parts of compound algae, 10 parts of compound amino acid stock solution, 5 parts of compound enzyme, 5 parts of boric acid, 5 parts of fermentation compound bacteria, 5 parts of zinc sulfate heptahydrate, 3 parts of zeolite powder, 3 parts of sodium dodecyl sulfate, 1 part of shell powder, 1 part of peregal and 1 part of water by using proportioning equipment, storing the weighed components by a storage device for later use, wherein the composite algae is a composition of brown algae, red algae and diatoms, the composite amino acid stock solution is a composition of lysine, methionine, threonine, aspartic acid and serine, the composite enzyme is a composition of glucanase, cellobiohydrolase and 1, 4-beta-mannuronic acid lyase, the fermentation composite bacteria is a composition of aspergillus niger, bacillus licheniformis, neurospora crassa and bacillus coagulans, and the composite algae after being cleaned and purified is dehydrated until the water content is 20 wt%;
s2, crushing treatment: pouring the fresh composite algae weighed in the step S1 into cleaning equipment, repeatedly washing for 3 times by using clear water, filtering impurities, dehydrating, freezing at-15 ℃ for 3 hours, taking out, and fully crushing the frozen composite algae by using crushing equipment, wherein the mass ratio of the feed liquid is 1: 0.5 adding clear water, and stirring at a rotation speed of 300r/min by a stirring device to prepare homogenate, wherein the crushing device adopts a tissue crusher with a model number of ZOLLO-UP 250;
s3, enzymolysis and fermentation treatment: adding the compound enzyme weighed in the step S1 into the homogenate obtained in the step S2, starting a stirring device to carry out enzymolysis for 10 hours at the rotating speed of 200r/min, separating enzymolysis liquid and residues through a filtering device after the enzymolysis is finished, mixing the fermentation compound bacteria weighed in the step S1 with the residues through a mixing stirring device, and fermenting for 15 hours according to fermentation conditions, wherein the fermentation conditions are as follows: the fermentation temperature is 25 ℃, and the fermentation pH is 5.3;
s4, primary mixing: mixing the enzymolysis liquid obtained in the step S3 with the fermentation product obtained in the step S3, respectively adding the compound amino acid stock solution, the zeolite powder and the shell powder, and uniformly stirring at the rotating speed of 600r/min by using stirring equipment;
s5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture primarily mixed in the step S4, continuously mixing and stirring for 1h at the rotating speed of 800r/min, and then transferring to a drying and granulating device for drying and granulating to obtain the composite algae bait.
Example 3
S1, weighing raw materials: firstly, respectively weighing 20 parts of compound algae, 20 parts of compound amino acid stock solution, 10 parts of compound enzyme, 10 parts of boric acid, 10 parts of fermentation compound bacteria, 10 parts of zinc sulfate heptahydrate, 5 parts of zeolite powder, 5 parts of lauryl sodium sulfate, 3 parts of shell powder, 3 parts of peregal and 3 parts of water by using a batching device, storing the weighed components by using a storage device for later use, wherein the compound algae is a composition of green algae, gulfweed and fucus, the compound amino acid stock solution is a composition of serine, glutamic acid, alanine, cystine and tyrosine, the compound enzyme is a composition of bromelain and saccharifying enzyme, the fermentation compound bacteria is a composition of bacillus subtilis, bacillus natto and bacillus megaterium, and dehydrating the compound algae after cleaning and impurity removal until the water content is 30 wt%;
s2, crushing treatment: pouring the fresh composite algae weighed in the step S1 into a cleaning device, repeatedly washing for 2 times by clear water, filtering out impurities, dehydrating, freezing at-25 ℃ for 5 hours, taking out, and fully crushing the frozen composite algae by a crushing device according to the mass ratio of the feed liquid of 1: 0.7, adding clear water, and stirring at a rotation speed of 500r/min by a stirring device to prepare homogenate, wherein the crushing device adopts a tissue crusher with a model number of ZOLLO-UP 250;
s3, enzymolysis and fermentation treatment: adding the compound enzyme weighed in the step S1 into the homogenate obtained in the step S2, starting a stirring device to carry out enzymolysis for 13 hours at the rotating speed of 300r/min, separating enzymolysis liquid and residues through a filtering device after the enzymolysis is finished, mixing the fermentation compound bacteria weighed in the step S1 with the residues through a mixing stirring device, and fermenting for 22 hours according to fermentation conditions, wherein the fermentation conditions are as follows: the fermentation temperature is 45 ℃ and the fermentation pH is 7.6;
s4, primary mixing: mixing the enzymolysis liquid obtained in the step S3 with the fermentation product obtained in the step S3, respectively adding the compound amino acid stock solution, the zeolite powder and the shell powder, and uniformly stirring at the rotating speed of 700r/min by using stirring equipment;
s5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture primarily mixed in the step S4, continuously mixing and stirring for 2 hours at the rotating speed of 1000r/min, and then transferring to a drying and granulating device for drying and granulating to obtain the composite algae bait.
In conclusion, the compound amino acid compound algae bait can supplement more abundant amino acid to aquatic animals by adding the compound amino acid into the bait, so that the compound algae bait is richer in nutrition, can realize rapid and sufficient enzymolysis and fermentation of the compound algae bait by adopting the compound enzyme and the compound bacteria for matching, well achieves the aim of rapidly converting and absorbing effective components in the compound algae by adding boric acid, zinc sulfate heptahydrate and sodium dodecyl sulfate, and is very beneficial to cultivation of production animals.
And those not described in detail in this specification are well within the skill of those in the art.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (8)
1. A method for producing natural bait low-temperature composite algae of aquatic animals is characterized by comprising the following steps: the method specifically comprises the following steps:
s1, weighing raw materials: firstly, respectively weighing 10-20 parts of compound algae, 10-20 parts of compound amino acid stock solution, 5-10 parts of compound enzyme, 5-10 parts of boric acid, 5-10 parts of fermentation compound bacteria, 5-10 parts of zinc sulfate heptahydrate, 3-5 parts of zeolite powder, 3-5 parts of sodium dodecyl sulfate, 1-3 parts of shell powder, 1-3 parts of peregal and 1-3 parts of water by using a batching device, and storing the weighed components by using a storage device for later use;
s2, crushing treatment: pouring the fresh composite algae weighed in the step S1 into cleaning equipment, repeatedly washing for 2-3 times by using clear water, filtering out impurities, dehydrating, freezing at-15 ℃ to-25 ℃ for 3-5h, taking out, and fully crushing the frozen composite algae by using crushing equipment, wherein the mass ratio of the feed liquid is 1: 0.5-0.7, adding clear water, and stirring by a stirring device at a rotating speed of 300-500r/min to prepare homogenate;
s3, enzymolysis and fermentation treatment: adding the complex enzyme weighed in the step S1 into the homogenate obtained in the step S2, starting a stirring device to carry out enzymolysis for 10-13h at the rotation speed of 200-300r/min, separating enzymolysis liquid and residues through a filtering device after the enzymolysis is finished, mixing the fermentation complex bacteria weighed in the step S1 with the residues through the mixing stirring device, and fermenting for 15-22h according to fermentation conditions;
s4, primary mixing: mixing the enzymolysis liquid obtained in the step S3 with the fermentation product obtained in the step S3, respectively adding the compound amino acid stock solution, the zeolite powder and the shell powder, and uniformly stirring at the rotating speed of 600-700r/min by using stirring equipment;
s5, secondary mixing: respectively adding the boric acid, the zinc sulfate heptahydrate, the sodium dodecyl sulfate, the peregal and the rest water weighed in the step S1 into the mixture primarily mixed in the step S4, continuously mixing and stirring for 1-2h at the rotating speed of 800-.
2. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the composite algae in step S1 is any combination of two or more of brown algae, red algae, diatom, green algae, gulfweed, or sea oak.
3. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the composite amino acid stock solution in the step S1 is any combination of two or more of lysine, methionine, threonine, aspartic acid, serine, glutamic acid, alanine, cystine, or tyrosine.
4. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the complex enzyme in the step S1 is any combination of two or more of glucanase, cellobiohydrolase, 1, 4-beta-mannuronic acid lyase, bromelain or glucoamylase.
5. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the fermentation composite bacteria in the step S1 are any combination of two or more of Aspergillus niger, Bacillus licheniformis, Neurospora crassa, Bacillus coagulans, Bacillus subtilis, Bacillus natto or Bacillus megaterium.
6. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the crushing device in step S2 is a tissue crusher with model number ZOLLO-UP 250.
7. The method for producing the natural bait low-temperature compound algae for the aquatic animals according to claim 1, characterized in that: and step S1, dehydrating the cleaned and impurity-removed compound algae until the water content is 20-30 wt%.
8. The method for producing the aquatic animal natural bait low-temperature compound algae as claimed in claim 1, wherein the method comprises the following steps: the fermentation conditions in the step S3 are: the fermentation temperature is 25-45 deg.C, and the fermentation pH is 5.3-7.6.
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| CN110338278A (en) * | 2019-07-30 | 2019-10-18 | 威海长青海洋科技股份有限公司 | One main laminaria fermented feed containing probiotics and preparation method thereof |
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