CN106260662A - A kind of preparation method of enzymatic hydrolysis and fermentation type premix material - Google Patents
A kind of preparation method of enzymatic hydrolysis and fermentation type premix material Download PDFInfo
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Abstract
The invention belongs to feedstuff, enzyme preparation and technical field of microbial fermentation, be specifically related to the preparation method of a kind of enzymatic hydrolysis and fermentation type premix material.After feedstuff, stone powder, compound microelement, compound enzyme, complex microorganism are mixed homogeneously with clean water by proportion, the natural fermentation some time, dry and after pulverizing, prepare enzymatic hydrolysis and fermentation culture medium butt；In proportion by after enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, aminoacids complex and functional feedstuff additive mix homogeneously, prepare enzymatic hydrolysis and fermentation type premix material.Enzymatic hydrolysis and fermentation type premix material of the present invention is with enzymatic hydrolysis and fermentation feedstuff as carrier, rich in vitamin, calcium lactate, organic trace element, lactic acid, little peptide, aminoacid and probiotic bacteria, there is the features such as high, easy to digest, the good palatability of nutrition, livestock and poultry production performance can be significantly improved, substantially increase feed intake, significantly reduce piglet diarrhea.
Description
Technical field
The present invention relates to feedstuff, enzyme preparation and technical field of microbial fermentation, be specially a kind of enzymatic hydrolysis and fermentation type premix material
Preparation method.
Background technology
China is populous nation, is also that the first in the world is raised pigs big country, and within 2015, the Animal Husbandry in China output value reaches 3.5 trillion yuan,
Live pig delivers 6.43 hundred million for sale, wherein sow livestock on hand 45,000,000, Carnis Sus domestica yield 54,870,000 tons.According to Ministry of Agriculture's statistical data,
Within 2015, national commercial feed yield reaches 2.18 hundred million tons, wherein complete feed 1.79 hundred million tons, concentrated feed 21,510,000 tons, premix
Expect 6,400,000 tons, including pig feed yield 89,740,000 tons, fowl material yield 90,000,000 tons, aquatic feeds 53,000,000 tons, cattle and sheep feedstuff 850
Ten thousand tons, 3,500,000 tons of the feedstuff such as rabbit Nyctereutes procyonoides house pet, whole nation sow feed more than 500 ten thousand tons.Expect the year two thousand twenty whole nation mixed feed yield
Being up to more than 2.2 hundred million tons, wherein sow feed is up to more than more than 800 ten thousand tons.
It is known that the exploitation of feed product, the difficult point of development and focus on premix material, so-called premix material is same class
Multiple additives or the most formulated homogeneous mixture of different types of multiple additives, though premix material is being joined
Close general proportion in feedstuff the least, only account for 0.5% 5%, mainly contain vitamin, macro minerals element (calcium, phosphorus and chlorination
Sodium), trace quantity mineral substance element, aminoacid and some medicated premixs, be the essential part constituting concentrated feed, be that full price coordinates and raises
The nucleus of material.
Nutrition composition in premix material must be dispersed in feedstuff, in order to ensure vitamin, trace ore deposit in premix material
Matter-element element and the coefficient of variation of some medicated premixs is little and the uniformity is high, it is necessary to by vitamin, trace quantity mineral substance element and some
Medicated premix is adsorbed in carrier and the diluent of premix material.Premix carrier refers to carry trace active composition, changes
Its dispersibility kind, and have good chemical stability and the raised material of adsorptivity.Carrier energy adsorption activity composition, makes activity one-tenth
The granule divided strengthens.After the carrying of micro constitutent loaded body, the some physical characteristics of itself change or no longer show,
Not only dilute micro constitutent, play the effect of diluent, also can improve the free-running property of additive, make additive be easier to uniformly
Being distributed in feedstuff, premix material diluent refers to be incorporated into the material playing diluting effect in one or more trace mineral supplements,
It can dilute the concentration of active component, but the physical characteristic of microcomponent will not occur significantly to change, and diluent does not rise and holds
Carrying the effect of additive, from granularity, it is thinner than carrier, and particle surface is more smooth, and free-running property is more preferable.
For at present research and application, premix carrier and diluent mainly have two big classes:
One, organic carrier and diluent
1, organic carrier
Organic carrier typically refers to those and contains the more plant of crude fibre and grain by-product, such as Testa Tritici, corn bran, corn cob
Powder, corn plumule powder, defatted rice bran, powdered rice hulls, soybean meal, groundnut stem meal, peanut hull meal, soybean stem meal, maize alcohol lees, grass
Powder, fecula, leaf powder etc..
2, organic diluent
Organic diluent mainly includes the de-Semen Maydis powder of embryo, glucose, sucrose, Semen sojae atricolor (parched) powder, secondary flour, Zein powder etc..
Do not emphasize load-carrying properties due to diluent, and compare emphasis and micro constitutent unit weight and the concordance of granularity.Therefore, selection is former
Material mostly is the feed ingredient of some routines.
The advantage of organic carrier and diluent be nontoxic, cheap, source is wide, but the easily moisture absorption, use previous as to dry
Processing, or with adsorbent equilibrium water conten without drying, such as adding bentonite, silicon dioxide or silicate etc. has and adsorbs more by force
The mineral of ability balances.If these organic carriers or diluent fat content are higher, need to carry out ungrease treatment, no
Then easy oxidation deterioration storing when, affects the quality of premix material.
Two, inorganic carrier and diluent
1, inorganic carrier
Substantially can be divided into two classes, one is calcium salt class；It two is the oxide-based of silicon.
(1), calcium salt class
Advantage is to enter after animal body with feed additive, has good effect to animal, typically will not cause poisoning, but
It is noted that the balance of Calcium in Feed.Mainly include following several: 1. precipitated calcium carbonate；2. natural whiting；3. bone tankage；
4. calcium phosphate；5. conch meal.
(2), the oxide of silicon is as the carrier of premix material
This material does not interferes with the nutritive equilibrium of mixed feed, and animal experiment also confirms that in feedstuff it is safe.This material
Sq content is high, therefore can prevent caking；It is added in feedstuff, due to their adsorptivity and dissolution characteristic, animal is had necessarily
Growth promoting function.Mainly include following several: 1. silicon dioxide (white carbon)；2. kieselguhr；3. zeolite powder；4. white pottery
Soil；5. meerschaum；6. bentonite；7. Maifanitum.
In this kind of carrier, the absorbability of some material is more weak, such as conch meal, stone powder etc., can classify as dilution
Agent.
2, inorganic diluents
Inorganic diluents includes dalcium biphosphate, agstone, conch meal, Kaolin, sodium sulfate, Sal etc..For diluent
Requirement emphasize that granularity, unit weight are close with micro constitutent.
Three, common carrier and the comparison of diluent
The Performance comparision of several common carrier: in organic carrier, divides after Fructus Hordei Vulgaris powder, bean cake powder and maize cob meal carrying micro constitutent
Level phenomenon is serious, and rice chaff powder also has certain classification phenomenon, but it is too many containing crude fibre, and uncomfortable cooperation carrier uses, and compares it
Under, Testa Tritici and sizing are more satisfactory；In inorganic carrier, middling flour classification is serious, and Sal hygroscopicity is strong, mobility
Difference, consumption is limited, uncomfortable cooperation carrier；Microlith powder makees carrier ratio conveniently.
The vibration that several common carrier and diluent mix after 20min with the micro constitutent such as crystal violet and ferrous sulfate is divided
Level and falling condition compare, and in carrier, defatted rice bran and wheat bran are all excellent, have good bearing capacity, are carried
Trace active composition be difficult to classification.The two is compared, and defatted rice bran unit weight is more than wheat bran, and granularity is relatively thin, (raw with dimension to crystal violet
Element density is suitable) and ferrous sulfate have good bearing capacity, thus can be as trace element, vitamin and compound additive
The preferred raw materials of premix carrier.Wheat bran is poor to the bearing capacity of ferrous sulfate, is easier to classification phenomenon.Semen Maydis powder unit weight
Greatly, smooth surface, bearing capacity are not so good as defatted rice bran and wheat bran, and coarse size person's effect is worse.Thin Semen Maydis powder be applicable to produce away from
Secondary premix carrier in factory short, that classification is the most serious.Three kinds of diluent all show after mixing with ferrous sulfate and crystal violet
The classification phenomenon of the latter is more than the former, particularly middling flour and conch meal, it is seen that these three material all can be pre-as trace element
The diluent of batch mixing, in three kinds of materials, the effect that dry powder makees diluent is best.But, wheat bran, Testa oryzae, Semen Maydis powder etc. are organic
Whether can be the most unknown as carrier after carrier enzymatic hydrolysis and fermentation, the most also nobody inquires into or application enzymatic hydrolysis and fermentation feedstuff
As premix carrier.
Enzymatic hydrolysis and fermentation feedstuff is with microorganism, compound enzyme for biological feed fermentation agent strain, is converted into by feedstuff micro-
Biological tropina, bioactive micro peptide amino acid, microbial activity probiotic bacteria, compound enzymic preparation are integrated biofermentation and raise
Material.Fermented feed is not only suitable for the livestock animals such as pig, cattle, sheep, is applicable to again the poultry animal such as chicken, duck, goose, and is applicable to fish
The various animals such as shrimp Aquatic product, tenebrio molitor insecticide.Fermented feed can make feedstuff removing toxic substances detoxification, is greatly improved the safety of feedstuff, and
And light fragrance can be produced during fermentation, thus add the palatability of animal.Sweat can also generate many
Plant organic acid, vitamin, enzyme, aminoacid and other multiple UGFs, substantially increase the nutrition of fermented feed
Level and digestive utilization ratio.Meanwhile, fermented feed also improves the resistance of animal, reduces feed cost.But, the most both at home and abroad
Fermented feed separates to isolate with premix material always and comes, and how to be got up with premix material organic assembling by enzymatic hydrolysis and fermentation feedstuff, and real
The two interaction potentiation existing is a job with wide application prospect.
The present invention, by after feed fermentation, enzymolysis, is dried and is prepared fermented feed butt, then using fermented feed butt as premix
Material carrier and diluent, in proportion with commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, compound
After aminoacid and functional feedstuff additive mix homogeneously, prepare enzymatic hydrolysis and fermentation type premix material.
Summary of the invention
It is an object of the invention to provide the preparation method of a kind of enzymatic hydrolysis and fermentation type premix material, and rich in vitamin, calcium lactate,
Organic trace element, lactic acid, little peptide, aminoacid and probiotic bacteria, have the features such as high, easy to digest, the good palatability of nutrition, can be substantially
Improve livestock and poultry production performance, hence it is evident that increase feed intake, significantly reduce the enzymatic hydrolysis and fermentation type premix material of piglet diarrhea.
The technical solution adopted in the present invention is:
A kind of preparation method of enzymatic hydrolysis and fermentation type premix material, step is as follows:
(1), the preparation of enzymatic hydrolysis and fermentation culture medium butt:
After feedstuff, stone powder, compound microelement, compound enzyme, complex microorganism are mixed homogeneously with clean water in proportion, from
So ferment the scheduled time, dry and after pulverizing, prepare enzymatic hydrolysis and fermentation culture medium butt；
(2), the preparation of enzymatic hydrolysis and fermentation type premix material:
By enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, answer
Close aminoacid and functional feedstuff additive mixes in proportion, prepare enzymatic hydrolysis and fermentation type premix material.
Preferably, during step (1) described feedstuff is National agricultural portion feedstuff catalogue feedstuff one or
Multiple, predominantly bean cake, cottonseed meal, rapeseed meal, Semen arachidis hypogaeae dregs, fish flour, blood powder, plasma proteins, hyperglobulinemia, milk powder, lactose, breast
Clear powder, wheat gluten flour, Zein powder, rice protein powder, DDGS, Semen sojae atricolor, Semen Pisi sativi, Semen Phaseoli, Semen Maydis, Oryza glutinosa, crack rice, little
Wheat, wheat bran, Testa oryzae, flour, Herba bromi japonici, Fructus Hordei Vulgaris, Maninot esculenta crantz., Radix Ipomoeae, soybean protein isolate, soybean protein concentrate, pasture powder, medium-height grass
One in medicine, fruit and vegetable, sugar molasse syrup and food processing industry side-product, or the dilated product of above-mentioned feedstuff
Or it is multiple；
Step (1) described compound microelement is the one or many of inorganic microelement in National agricultural portion feed additive catalogue
Kind, predominantly ferrous sulfate, ferrous chloride, iron chloride, ferrous carbonate, copper chloride, copper sulfate, copper chloride hydroxide, zinc oxide, chlorine
Change zinc, zinc carbonate, zinc sulfate, zinc acetate, basic zinc chloride, manganese chloride, manganese oxide, manganese sulfate, manganese carbonate, manganese hydrogen phosphate, iodine
Change one or more in potassium, sodium iodide, potassium iodate, calcium iodate, cobaltous chloride, cobalt acetate, cobaltous sulfate, sodium selenite；
Step (1) described compound enzyme be in National agricultural portion feed additive catalogue feeding enzyme one or more, predominantly form sediment
Powder enzyme, cellulase, protease, glucanase, xylanase, pectase, phytase, maltase, glucoseoxidase, wheat
One or more in bud carbohydrase, lipase；
Step (1) described complex microorganism is commercially available composite bacteria, for micro-life feeding in National agricultural portion feed additive catalogue
One or more of thing, predominantly bacillus subtilis, Bacillus licheniformis, bifidobacterium bifidum, enterococcus faecalis, dung intestinal ball
Bacterium, lactoenterococcus, bacillus acidophilus, lactobacillus casei, moral formula Lactobacillus lactate subspecies, Lactobacillus plantarum, pediococcus acidilactici,
Pediococcus pentosaceus, Candida utilis, saccharomyces cerevisiae, Rhodopseudomonas palustris, bifidobacteria infantis, bifidobacterium longum, short double
Discrimination bacillus, bifidobacterium adolescentis, streptococcus thermophilus, Lactobacillus reuteri, animal bifidobacteria, aspergillus niger, aspergillus oryzae, slow bud
One in spore bacillus, Bacillus pumilus, lactobacillus cellobiosas, Lactobacillus fermenti, lactobacillus delbruockii subspecies bulgaricus
Or it is multiple；
Step (2) described compound vitamin be in National agricultural portion feed additive catalogue vitamin one or more, mainly
For vitamin A, retinyl acetate, vitamin A palmitate, beta-carotene, thiamine hydrochloride (vitamin B1), thiamine mononitrate
(vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cobalamin (vitamin B12), L-AA
(vitamin C), L-AA calcium, L-AA sodium, L-AA-2-phosphate ester, L-AA-6-Palmic acid
Ester, vitamin D2, vitamin D3, natural Vitamin E, dl-alpha-tocopherol, dl-alpha-tocopherol acetate, sodium sulfite first naphthalene
Quinone (vitamin K3), dimethyl pyrimidine alcohol sulfurous acid menadione, bisulfite nicotiamide menadione, nicotinic acid, nicotiamide, D-are general
Alcohol, D-VB5 calcium, DL-calcium pantothenate, folic acid, Bio, choline chloride, inositol, VBT, VBT hydrochlorate, glycine betaine,
One or more of beet alkali hydrochlorate, 25-hydroxvcholecalciferol and VBT tartrate；
Step (2) described aminoacids complex be in National agricultural portion feed additive catalogue amino acid whose one or more, mainly
For 1B, methionine hydroxy analog, methionine hydroxy analog calcium salt, L-lysine hydrochloride, 1B sulphuric acid
Salt and fermentation byproduct thereof, DL-methionine, L-threonine, L-Trp, L-arginine, L-arginine hydrochlorate, glycine,
TYR, ALANINE, sky (door) winter propylhomoserin, L-Leu, isoleucine, L-PROLINE, phenylalanine, serine, L-
Cysteine, L-Histidine, glutamic acid, glutamine, valine, cystine, taurine one or more；
Step (2) described functional feedstuff additive is National agricultural portion feed additive catalogue Chinese medicine, antioxidant, anticorrosion
One or more of agent, antifungus agent, acidity regulator, seasoning Attractant, polysaccharide and oligosaccharide.
Preferably, during step (1) described feedstuff is National agricultural portion feedstuff catalogue feedstuff one or
Multiple, predominantly bean cake, Zein powder, DDGS, wheat bran, Testa oryzae, molasses one or more；
Step (1) described compound microelement is the one or many of inorganic microelement in National agricultural portion feed additive catalogue
Kind, predominantly one or more in ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide, cobaltous chloride, sodium selenite；
Step (1) described compound enzyme be in National agricultural portion feed additive catalogue feeding enzyme one or more, predominantly form sediment
One or more in powder enzyme, cellulase, protease, glucanase, xylanase, pectase, phytase；
Step (1) described complex microorganism is commercially available composite bacteria, for micro-life feeding in National agricultural portion feed additive catalogue
One in one or more of thing, predominantly bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, saccharomyces cerevisiae or
Multiple；
Step (2) described compound vitamin be in National agricultural portion feed additive catalogue vitamin one or more, mainly
For vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cobalamin (dimension
Raw element B12), L-AA (vitamin C), vitamin D2, vitamin D3, natural Vitamin E, menadione sodium bisulfite
(vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, choline chloride one or more；
Step (2) described aminoacids complex be in National agricultural portion feed additive catalogue amino acid whose one or more, mainly
For L-lysine hydrochloride, DL-methionine, L-threonine, L-Trp one or more；
Preferably, antioxidant during step (2) described functional feedstuff additive is National agricultural portion feed additive catalogue, anti-
Rotten agent, antifungus agent, seasoning Attractant one or more.
Preferably, in step (1), the preparation condition of enzymatic hydrolysis and fermentation culture medium butt is as follows:
The weight ratio of feedstuff, stone powder, compound microelement, compound enzyme, commercially available composite bacteria and clean water be 100:0.1～
50:0.01～10:0.005～5:0.1～50:1～1000；
Fermentation temperature: 2～60 DEG C；
Starting fermentation pH: natural；
Fermentation time: 2～480 hours；
Smashing fineness after drying: 5～1000 mesh.
Preferably, the preparation method of a kind of enzymatic hydrolysis and fermentation type premix material:
Feedstuff, stone powder, compound microelement, compound enzyme, complex microorganism are 100:5～10 with the weight ratio of clean water:
0.5～1:0.1～0.5:5～10:30～60；
Fermentation temperature: 25～35 DEG C；
Starting fermentation pH: natural；
Fermentation time: 72～96 hours；
Smashing fineness after drying: 80～120 mesh；
Preferably, step (1) is described when preparing enzymatic hydrolysis and fermentation culture medium butt:
Each constituent of feedstuff and weight ratio be: bean cake, Zein powder, DDGS, wheat bran, Testa oryzae, the weight of molasses
Ratio is 100:0～300:0～300:0～1000:0～1000:0～500；
Each constituent of compound microelement and weight ratio be: ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide,
Cobaltous chloride, the weight ratio of sodium selenite are 100:0～500:0～300:0～200:0～10:0～5:0～5；
Each constituent of compound enzyme and weight ratio be: amylase, cellulase, protease, glucanase, xylanase, really
Glue enzyme, the weight ratio of phytase are 100:0～500:0～300:0～200:0～200:0～200:0～200；
Each constituent of complex microorganism and weight ratio be: bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, wine
The weight ratio of brewer yeast is 100:0～800:0～500:0～300.
Preferably, when step (2) prepares enzymatic hydrolysis and fermentation type premix material:
Each constituent of compound vitamin and weight ratio be: vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin
B2), pyridoxine hydrochloride (vitamin B6), cobalamin (vitamin B12), L-AA (vitamin C), vitamin D2, dimension life
Element D3, natural Vitamin E, menadione sodium bisulfite (vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, chlorination
The weight ratio of choline is 100:0～500:0～300:0～200:0～200:0～2000:0～200:0～500:0～300:0
～200:0～200:0～200:0～100:0～100:0～2000；
Each constituent of aminoacids complex and weight ratio be: L-lysine hydrochloride, DL-methionine, L-threonine and L-color
The weight ratio of propylhomoserin is 100:0～500:0～300:0～100.
Preferably, step (1) is described when preparing enzymatic hydrolysis and fermentation culture medium butt:
Each constituent of feedstuff and weight ratio be: bean cake, Zein powder, DDGS, wheat bran, Testa oryzae, the weight of molasses
Ratio is 100:10～20:5～10:60～80:60～80:20～30；
Each constituent of compound microelement and weight ratio be: ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide,
Cobaltous chloride, the weight ratio of sodium selenite be 100:60～80:40～60:20～30:0.05～0.2:0.01～0.1:0.01～
0.1；
Each constituent of compound enzyme and weight ratio be: amylase, cellulase, protease, glucanase, xylanase, really
Glue enzyme, the weight ratio of phytase are 100:0～50:0～30:0～20:0～20:0～20:0～20；
Each constituent of complex microorganism and weight ratio be: bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, wine
The weight ratio of brewer yeast is 100:50～60:30～50:20～30；
Preferably, when step (2) prepares enzymatic hydrolysis and fermentation type premix material:
Each constituent of compound vitamin and weight ratio be: vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin
B2), pyridoxine hydrochloride (vitamin B6), cobalamin (vitamin B12), L-AA (vitamin C), vitamin D2, dimension life
Element D3, natural Vitamin E, menadione sodium bisulfite (vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, chlorination
The weight ratio of choline is 100:30～50:20～30:10～20:10～20:100～200:10～20:40～50:15～30:
10～20:10～20:10～20:5～10:3～5:100～200；
Each constituent of aminoacids complex and weight ratio be: L-lysine hydrochloride, DL-methionine, L-threonine and L-color
The weight ratio of propylhomoserin is 100:80～120:30～60:5～10；
Preferably, the preparation condition of described enzymatic hydrolysis and fermentation type premix material is as follows:
Enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, compound
The weight ratio of aminoacid and functional feedstuff additive be 100:0～1200:0～800:0～100:0～50:0～500:0～
1000:0～100.
Preferably, the preparation condition of described enzymatic hydrolysis and fermentation type premix material is as follows:
Enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, compound
The weight ratio of aminoacid and functional feedstuff additive is 100:80～120:60～80:0.4～4:0.2～2:10～20:30
～60:2～5.
The invention has the beneficial effects as follows: zymolysis feedstuff prepared by the present invention is with enzymatic hydrolysis and fermentation feedstuff as carrier, raw rich in dimension
Element, calcium lactate, organic trace element, lactic acid, little peptide, aminoacid and probiotic bacteria, have high, easy to digest, the good palatability of nutrition etc.
Feature, can significantly improve livestock and poultry production performance, hence it is evident that increases feed intake, significantly reduces piglet diarrhea.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement
Example is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this area is common
The every other embodiment that technical staff is obtained under not making creative work premise, broadly falls into the model of present invention protection
Enclose.
Embodiment 1
1., the preparation of enzymatic hydrolysis and fermentation culture medium butt:
By 100kg bean cake, 15kg Zein powder, 8kg DDGS, 70kg wheat bran, 70kg Testa oryzae, 22kg molasses, 20kg stone powder,
After 2kg compound microelement, 0.5kg compound enzyme, the commercially available composite bacteria of 30kg are mixed homogeneously with 150kg clean water, at 28-32
Under the conditions of DEG C, carry out natural fermentation 60 hours, after preparing 470-480kg enzymatic hydrolysis and fermentation culture medium wet basis, then dry pulverizing 80 mesh,
Prepare 270-280kg enzymatic hydrolysis and fermentation culture medium butt.
2., the preparation of enzymatic hydrolysis and fermentation type premix material:
270-280kg enzymatic hydrolysis and fermentation culture medium butt, the commercially available stone powder of 350kg, 180kg calcium hydrogen phosphate, 7.5kg sodium chloride, 2kg are multiple
After closing vitamin, 60kg compound microelement, 100kg aminoacids complex and 12kg functional feedstuff additive mix homogeneously, system
Obtain 980-990kg enzymatic hydrolysis and fermentation type premix material.
Embodiment 2
1., the preparation of enzymatic hydrolysis and fermentation culture medium butt:
By multiple to 100kg bean cake, 80kg wheat bran, 100kg Testa oryzae, 30kg molasses, 30kg stone powder, 2kg compound microelement, 0.5kg
After the commercially available composite bacteria of synthase, 30kg is mixed homogeneously with 200kg clean water, under the conditions of 30-35 DEG C, carry out natural fermentation 96
Hour, after preparing 550-560kg enzymatic hydrolysis and fermentation culture medium wet basis, then dry pulverizing 120 mesh, prepare 280-290kg enzymatic hydrolysis and fermentation
Culture medium butt.
2., the preparation of enzymatic hydrolysis and fermentation type premix material:
280-290kg enzymatic hydrolysis and fermentation culture medium butt, the commercially available stone powder of 300kg, 150kg calcium hydrogen phosphate, 10kg sodium chloride, 3kg are multiple
After closing vitamin, 60kg compound microelement, 150kg aminoacids complex and 15kg functional feedstuff additive mix homogeneously, system
Obtain 960-970kg enzymatic hydrolysis and fermentation type premix material.
Embodiment 3
1., the preparation of enzymatic hydrolysis and fermentation culture medium butt:
By 100kg bean cake, 50kg wheat bran, 20kg molasses, 30kg stone powder, 2kg compound microelement, 0.5kg compound enzyme, 20kg city
Sell after composite bacteria mixs homogeneously with 80kg clean water, under the conditions of 20-25 DEG C, carry out natural fermentation 72 hours, prepare 280-
After 290kg enzymatic hydrolysis and fermentation culture medium wet basis, then dry pulverizing 80 mesh, prepare 140-145kg enzymatic hydrolysis and fermentation culture medium butt.
2., the preparation of enzymatic hydrolysis and fermentation type premix material:
140-145kg enzymatic hydrolysis and fermentation culture medium butt, the commercially available stone powder of 150kg, 80kg calcium hydrogen phosphate, 4kg sodium chloride, 2kg are compound
After vitamin, 30kg compound microelement, 70kg aminoacids complex and 6kg functional feedstuff additive mix homogeneously, prepare
480-485kg enzymatic hydrolysis and fermentation type premix material.
Embodiment 4
1., the preparation of enzymatic hydrolysis and fermentation culture medium butt:
By 200kg bean cake, 30kg Zein powder, 70kg Testa oryzae, 30kg molasses, 30kg stone powder, 3kg compound microelement,
After the commercially available composite bacteria of 0.8kg compound enzyme, 50kg is mixed homogeneously with 100kg clean water, under the conditions of 22-26 DEG C, carry out nature
Ferment 72 hours, after preparing 490-510kg enzymatic hydrolysis and fermentation culture medium wet basis, then dry pulverizing 80 mesh, prepare 290-300kg enzymolysis
Fermentation medium butt.
2., the preparation of enzymatic hydrolysis and fermentation type premix material:
290-300kg enzymatic hydrolysis and fermentation culture medium butt, the commercially available stone powder of 250kg, 150kg calcium hydrogen phosphate, 7.5kg sodium chloride, 2kg are multiple
After closing vitamin, 50kg compound microelement, 80kg aminoacids complex and 10kg functional feedstuff additive mix homogeneously, prepare
838-848kg enzymatic hydrolysis and fermentation type premix material.
Embodiment 5
1., the preparation of enzymatic hydrolysis and fermentation culture medium butt:
By multiple to 20kg bean cake, 100kg wheat bran, 100kg Testa oryzae, 50kg molasses, 20kg stone powder, 3kg compound microelement, 0.6kg
After the commercially available composite bacteria of synthase, 30kg is mixed homogeneously with 150kg clean water, under the conditions of 28-32 DEG C, carry out natural fermentation 60
Hour, after preparing 450-460kg enzymatic hydrolysis and fermentation culture medium wet basis, then dry pulverizing 80 mesh, prepare the training of 210-220kg enzymatic hydrolysis and fermentation
Support backbone base.
2., the preparation of enzymatic hydrolysis and fermentation type premix material:
210-220kg enzymatic hydrolysis and fermentation culture medium butt, the commercially available stone powder of 200kg, 120kg calcium hydrogen phosphate, 7.5kg sodium chloride, 2kg are multiple
After closing vitamin, 50kg compound microelement, 50kg aminoacids complex and 7.5kg functional feedstuff additive mix homogeneously, system
Obtain 645-647kg enzymatic hydrolysis and fermentation type premix material.
One, Major Nutrient material content in the enzymatic hydrolysis and fermentation type premix material of embodiment 1 preparation, is shown in Table 1.
The enzymatic hydrolysis and fermentation type premix material product Middle nutrition content of material of table 1, embodiment 1 preparation
| Project | Index | Project | Index | Project | Index |
| Color and luster | Yellowish-brown | Compound vitamin/% | ≥0.2 | Sodium chloride | ≥0.3 |
| Moisture/% | ≤8.0 | Compound microelement/% | ≥2.0 | Organic ore deposit/% | ≥0.1 |
| Crude protein/% | ≥12.0 | Calcium/% | ≥1.0 | Pfansteihl/% | ≥0.2 |
| Crude fat/% | ≥0.5 | Phosphorus/% | ≥0.50 | Total amount of probiotics cfu/g | ≥0.03×106 |
Two, the enzymatic hydrolysis and fermentation type premix material of embodiment 1 preparation feeds ablactational baby pig effect.
Feeding ablactational baby pig effect to inquire into the enzymatic hydrolysis and fermentation type premix material of embodiment 1 preparation, we select DLY
Three way cross piglet has been cooked following research, and result is as follows:
Test pig selects and packet: test pig selects the body weight close (body weight 18.9 ± 0.85kg) that ten thousand kind pig farms of Xu, north are raised
DLY three way cross piglet 168 (59 and 60 age in days piglet), be randomly divided into test group and matched group, test group 84
(sow 44, boar 40), matched group 84 (sow 38, boar 46).Two groups of close (significance analysis of pig weight
The most notable), each group is all provided with 7 repetitions, each repetition 12.
Test pig house is roof double-pitch type, swinery double-row type, cement flooring, and concrete hopper installs automatic drinking bowl, pig
House door and window very well equipped, ventilation and lighting is preferable.Raising phase diel in advance to feed 3 times, experimental period diel feeds 4 times, in 7 points, 11
Point, 15 points, 20 powders of manually throwing something and feeding.It is main for often eating not remain material.Pig house natural lighting on daytime, ventilation, night lights illumination,
Normal immunological and management.Pig house temperature controls to being not less than 15 DEG C, and humid control is 55 65%.Accomplish environmental condition one as far as possible
Cause, with the at a draft person of supporting management, free choice feeding and drinking-water.
Test feed and nutrition: test group is identical with matched group piglet basal diet nutrition, be powder, and matched group uses
Zeolite powder is the premix material of carrier, and test group uses enzymatic hydrolysis and fermentation type premix material, and i.e. using enzymatic hydrolysis and fermentation feedstuff is the pre-of carrier
Batch mixing.Stone powder in test group and matched group premix material, calcium hydrogen phosphate, compound vitamin, compound microelement, aminoacids complex
Completely the same with, functional feedstuff additive content, daily ration mix homogeneously, the coefficient of variation is less than 8% after measured.
Preliminary trial period: test and start prerun on 28th from December in 2015,10 days preliminary trial periods, carry out the works such as prevention, inoculation, anthelmintic
Making, prerun enters after terminating and just tries.
Just try the phase: test is from the beginning of on January 7th, 2016 is formally, and on March 1st, 2016 formally terminates, go through examination 54 days.
Result of the test is as follows:
42 piglets of test group averagely end weight 38.06kg, matched group averagely end weight 35.71kg, test group has more than matched group
2.35kg, improves 6.58%(P < 0.05)；Test group average weight gain 19.24kg, matched group average weight gain 16.66kg, test group
Have more 2.58kg than matched group, improve 15.49%(P < 0.01)；Test group average daily gain 356.30g, matched group average day
Weightening finish 308.52g, test group has more 47.78g than matched group, improves 15.49%(P < 0.01).Refer to table 2.
The table 2 enzymatic hydrolysis and fermentation type premix material unit that affects on ablactational baby pig daily gain: kg, g
| Group | Head number | Test natural law | Starting weight/head/kg | End weight/head kg | Daily gain/head/g |
| Matched group | 84 | 54 | 19.05 | 35.71±1.69 | 308.52±64 |
| Test group | 84 | 54 | 18.82 | 38.06±2.85* | 356.30±111** |
Note: * * represents the significance of difference (P < 0.01) in row, * represents the significance of difference (P < 0.05) in row.Table 2 is herewith.
Test group every feed intake 45.21 kg, matched group every feed intake 40.32 kg, test group is more than matched group
40.89 kg., feed intake adds 12.13%(P < 0.05)；Test group feed conversion rate 2.35, fewer than matched group 2.42 0.07,
Test group reduces 2.89%(P than matched group > 0.05).Refer to table 3.
Table 3 enzymatic hydrolysis and fermentation type premix material affects unit: kg, unit to ablactational baby pig feed intake and feedstuff-meat ratio
| Group | Head number | Weightening finish/head/kg | Feed intake/head/kg | Feed conversion rate | Feed cost/unit | Feed cost/unit // 1kg weightening finish |
| Matched group | 84 | 16.66 | 40.32±1.23 | 2.42±0.49 | 60.48 | 3.63 |
| Test group | 84 | 19.24 | 45.21±2.35* | 2.35±0.83 | 67.82 | 3.52 |
Note: by there and then feedstuff calculation of price, 1.508 yuan/kg of feedstuff.
Three, the enzymatic hydrolysis and fermentation type premix material of embodiment 2 preparation feeds fattening pig effect.
This test is by using enzymatic hydrolysis and fermentation type premix material to study it to Performance of Finishing Pigs at Diet For Finishing Pig
Impact.
Test period: on January 3rd, 2016 was to February 9
The selection of test swinery and packet
Selective body focuses on about 60 kilograms, and kind is the ternary market pig 240 of DLY, adjusts male and female by former hurdle equal as far as possible
Point.It is randomly divided into two groups, often group 120, often 10 repetitions of group, each repetition 12.Test period 35 days.
Test swinery free choice feeding dry mash, freely drinks water, and every day, timing cleaned health in hurdle, keeps clean.It is some other time
Often management is carried out routinely.
Test feed and nutrition: test group is identical with matched group piglet basal diet nutrition, be powder, and matched group uses
Powdered rice hulls is the premix material of carrier, and test group uses enzymatic hydrolysis and fermentation type premix material, and i.e. using enzymatic hydrolysis and fermentation feedstuff is the pre-of carrier
Batch mixing.Stone powder in test group and matched group premix material, calcium hydrogen phosphate, compound vitamin, compound microelement, aminoacids complex
Completely the same with, functional feedstuff additive content, daily ration mix homogeneously, the coefficient of variation is less than 8% after measured.
Result of the test is as follows:
The table 4 enzymatic hydrolysis and fermentation type premix material unit that affects on Performance of Finishing Pigs: kg, g
| Project | Matched group | Test group |
| Head number (head) | 50 | 50 |
| Initial weight (kg) | 3012.0 | 3003.0 |
| End weight (kg) | 4375.25 | 4418.75 |
| Total feed consumption rate (kg) | 4147.50 | 4060.0 |
| Average daily ingestion amount (kg) | 2.37 | 2.32 |
| Total augment weight (kg) | 1363.25 | 1415.75 |
| Average daily gain (g) | 779 | 809 |
| Efficiency of feed utilization | 3.04:1 | 2.87:1 |
| Sickness rate (%) | 8 | 0 |
As seen from the above table, matched group daily gain is 779 grams, and test group is 809 grams, and test group improves 3.85% than matched group；Right
Being 3.04:1 according to group efficiency of feed utilization, test group is 2.87:1, and test group improves 5.6% than matched group；Matched group average day adopts
Appetite is 2.37 kilograms, and test group average out to 2.32 kilograms, test group improves 2.1% than matched group.
Finally it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention,
Although being described in detail the present invention with reference to previous embodiment, for a person skilled in the art, it still may be used
So that the technical scheme described in foregoing embodiments to be modified, or wherein portion of techniques feature is carried out equivalent,
All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included in the present invention's
Within protection domain.
Claims (6)
1. the preparation method of an enzymatic hydrolysis and fermentation type premix material, it is characterised in that its step is as follows:
(1), the preparation of enzymatic hydrolysis and fermentation culture medium butt
After feedstuff, stone powder, compound microelement, compound enzyme, complex microorganism are mixed homogeneously with clean water in proportion, from
So ferment the scheduled time, dry and after pulverizing, prepare enzymatic hydrolysis and fermentation culture medium butt；
(2), the preparation of enzymatic hydrolysis and fermentation type premix material
Enzymatic hydrolysis and fermentation culture medium butt and commercially available stone powder that step (1) is prepared, calcium hydrogen phosphate, sodium chloride, compound vitamin, multiple
Close trace element, aminoacids complex and functional feedstuff additive to mix in proportion, prepare enzymatic hydrolysis and fermentation type premix material.
The preparation method of a kind of enzymatic hydrolysis and fermentation type premix material the most according to claim 1, it is characterised in that step (1) institute
State feedstuff be in National agricultural portion feedstuff catalogue feedstuff one or more, predominantly bean cake, zein
Powder, DDGS, wheat bran, Testa oryzae, molasses one or more；
Step (1) described compound microelement is the one or many of inorganic microelement in National agricultural portion feed additive catalogue
Kind, predominantly one or more in ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide, cobaltous chloride, sodium selenite；
Step (1) described compound enzyme be in National agricultural portion feed additive catalogue feeding enzyme one or more, predominantly form sediment
One or more in powder enzyme, cellulase, protease, glucanase, xylanase, pectase, phytase；
Step (1) described complex microorganism is commercially available composite bacteria, for micro-life feeding in National agricultural portion feed additive catalogue
One in one or more of thing, predominantly bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, saccharomyces cerevisiae or
Multiple；
Step (2) described compound vitamin be in National agricultural portion feed additive catalogue vitamin one or more, mainly
For vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin B2), pyridoxine hydrochloride (vitamin B6), cobalamin (dimension
Raw element B12), L-AA (vitamin C), vitamin D2, vitamin D3, natural Vitamin E, menadione sodium bisulfite
(vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, choline chloride one or more；
Step (2) described aminoacids complex be in National agricultural portion feed additive catalogue amino acid whose one or more, mainly
For L-lysine hydrochloride, DL-methionine, L-threonine, L-Trp one or more；
Step (2) described functional feedstuff additive is antioxidant in National agricultural portion feed additive catalogue, preservative, prevents
Mould dose, seasoning Attractant one or more.
The preparation method of a kind of enzymatic hydrolysis and fermentation type premix material the most according to claim 1, it is characterised in that step (1) is raised
Material raw material, stone powder, compound microelement, compound enzyme, commercially available composite bacteria are 100:0.1～50 with the weight ratio of clean water:
When 0.01～10:0.005～5:0.1～50:1～1000, the preparation condition of its enzymatic hydrolysis and fermentation culture medium butt is as follows:
Fermentation temperature: 2～60 DEG C；
Starting fermentation pH: natural；
Fermentation time: 2～480 hours；
Smashing fineness after drying: 5～1000 mesh；
Step (1) feedstuff, stone powder, compound microelement, compound enzyme, complex microorganism are 100 with the weight ratio of clean water:
When 5～10:0.5～1:0.1～0.5:5～10:30～60, the preparation condition of its enzymatic hydrolysis and fermentation culture medium butt is as follows:
Fermentation temperature: 25～35 DEG C；
Starting fermentation pH: natural；
Fermentation time: 72～96 hours；
Smashing fineness after drying: 80～120 mesh.
The preparation method of a kind of enzymatic hydrolysis and fermentation type premix material the most according to claim 1, it is characterised in that described step
(1) when preparing enzymatic hydrolysis and fermentation culture medium butt:
Each constituent of feedstuff and weight ratio be: bean cake, Zein powder, DDGS, wheat bran, Testa oryzae, the weight of molasses
Ratio is 100:0～300:0～300:0～1000:0～1000:0～500；
Each constituent of compound microelement and weight ratio be: ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide,
Cobaltous chloride, the weight ratio of sodium selenite are 100:0～500:0～300:0～200:0～10:0～5:0～5；
Each constituent of compound enzyme and weight ratio be: amylase, cellulase, protease, glucanase, xylanase, really
Glue enzyme, the weight ratio of phytase are 100:0～500:0～300:0～200:0～200:0～200:0～200；
Each constituent of complex microorganism and weight ratio be: bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, wine
The weight ratio of brewer yeast is 100:0～800:0～500:0～300；
When described step (2) prepares enzymatic hydrolysis and fermentation type premix material:
Each constituent of compound vitamin and weight ratio be: vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin
B2), pyridoxine hydrochloride (vitamin B6), cobalamin (vitamin B12), L-AA (vitamin C), vitamin D2, dimension life
Element D3, natural Vitamin E, menadione sodium bisulfite (vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, chlorination
The weight ratio of choline is 100:0～500:0～300:0～200:0～200:0～2000:0～200:0～500:0～300:0
～200:0～200:0～200:0～100:0～100:0～2000；
Each constituent of aminoacids complex and weight ratio be: L-lysine hydrochloride, DL-methionine, L-threonine and L-color
The weight ratio of propylhomoserin is 100:0～500:0～300:0～100.
The preparation method of a kind of enzymatic hydrolysis and fermentation type premix material the most according to claim 1, it is characterised in that described step
(1) when preparing enzymatic hydrolysis and fermentation culture medium butt:
Each constituent of feedstuff and weight ratio be: bean cake, Zein powder, DDGS, wheat bran, Testa oryzae, the weight of molasses
Ratio is 100:10～20:5～10:60～80:60～80:20～30；
Each constituent of compound microelement and weight ratio be: ferrous sulfate, copper sulfate, zinc sulfate, manganese sulfate, potassium iodide,
Cobaltous chloride, the weight ratio of sodium selenite be 100:60～80:40～60:20～30:0.05～0.2:0.01～0.1:0.01～
0.1；
Each constituent of compound enzyme and weight ratio be: amylase, cellulase, protease, glucanase, xylanase, really
Glue enzyme, the weight ratio of phytase are 100:0～50:0～30:0～20:0～20:0～20:0～20；
Each constituent of complex microorganism and weight ratio be: bacillus subtilis, Bacillus licheniformis, Lactobacillus plantarum, wine
The weight ratio of brewer yeast is 100:50～60:30～50:20～30；
When described step (2) prepares enzymatic hydrolysis and fermentation type premix material:
Each constituent of compound vitamin and weight ratio be: vitamin A, thiamine hydrochloride (vitamin B1), riboflavin (vitamin
B2), pyridoxine hydrochloride (vitamin B6), cobalamin (vitamin B12), L-AA (vitamin C), vitamin D2, dimension life
Element D3, natural Vitamin E, menadione sodium bisulfite (vitamin K3), nicotinic acid, D-VB5 calcium, folic acid, Bio, chlorination
The weight ratio of choline is 100:30～50:20～30:10～20:10～20:100～200:10～20:40～50:15～30:
10～20:10～20:10～20:5～10:3～5:100～200；
Each constituent of aminoacids complex and weight ratio be: L-lysine hydrochloride, DL-methionine, L-threonine and L-color
The weight ratio of propylhomoserin is 100:80～120:30～60:5～10.
The preparation method of a kind of enzymatic hydrolysis and fermentation type premix material the most according to claim 1, it is characterised in that described step
(2) preparation condition preparing enzymatic hydrolysis and fermentation type premix material is as follows:
Enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, compound
The weight ratio of aminoacid and functional feedstuff additive be 100:0～1200:0～800:0～100:0～50:0～500:0～
1000:0～100；
Enzymatic hydrolysis and fermentation culture medium butt, commercially available stone powder, calcium hydrogen phosphate, sodium chloride, compound vitamin, compound microelement, compound
The weight ratio of aminoacid and functional feedstuff additive is 100:80～120:60～80:0.4～4:0.2～2:10～20:30
～60:2～5.
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