CN106173205B - A kind of fermented type sweet potato dregs protein feed and the sheep cultural method using its progress - Google Patents
A kind of fermented type sweet potato dregs protein feed and the sheep cultural method using its progress Download PDFInfo
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- CN106173205B CN106173205B CN201610600790.6A CN201610600790A CN106173205B CN 106173205 B CN106173205 B CN 106173205B CN 201610600790 A CN201610600790 A CN 201610600790A CN 106173205 B CN106173205 B CN 106173205B
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The present invention relates to a kind of protein feed, in particular to a kind of fermented type sweet potato dregs protein feed and the sheep cultural method carried out using it.The present invention is using sweet potato dregs as raw material, and being mixed into mixed fermentation strain (decomposing mixed bacterium with starch fiber to mix with candida utili), ferment is made protein feed.In fermentation process, the much starch contained in sweet potato dregs and fiber degradation can be held digestible short-chain carbohydrates at animal by fermenting microbe, and fermenting microbe can also generate a large amount of mycoproteins in degradation process, further increase nutritive value.The ratio of fermentation step and fermenting microbe is adjusted, the content of mycoprotein in the degradation rate and tunning of starch and fiber in raw material is improved, to promote the deep processing of sweet potato to utilize.
Description
Technical field
The present invention relates to a kind of protein feed, in particular to a kind of fermented type sweet potato dregs protein feed and utilize its carry out
Sheep cultural method.
Background technique
The characteristics of sweet potato whole body is treasured, has cereal crops, industrial crops and medicinal crop concurrently.It is acted on widely, is
The important source material of the industry such as light industry, chemical industry, medicine, food, weaving.Sugar, citric acid, lactic acid, butyric acid, dimension life can be made in sweet potato
Plain and a variety of full powder derivative products.Such as in Zhejiang area, having developed mature product has pure white potato powder, instant noodles potato
Powder, puffed sweet potato chips, the extraction of edible pigment, roasting potato, potato dried meat etc..After fresh sweet potato passes through working process, the gained output value is higher than itself
Several times even more than ten times out.But with the scale of processing, how by-product in sweet potato starch industry is using gradually causing
The attention of people.While promoting the existing Sweet Potato Industry transformation of scientific and technical result, further to increase to by-product in industrial chain
The input in science and technology and dynamics of development and utilization, the deep processing research for carrying out Sweet Potato Industry chain by-product become the new starting point of research.
China is a resource micro state, and it is particularly problematic to strive grain by especially people and animals.The fast development of China's animal husbandry adds
The acute seriousness of grain unbalanced supply-demand, the Ministry of Agriculture point out the non-grain fodder resources such as herbage to be made full use of, agricultural and sideline product, especially
It is protein or the high feed resource of energy content, develops save-grain animal husbandry, and then alleviates China's grain supply and demand contradiction, protects
Hinder livestock products effective supply.It is China typical Suburb Areas of Big Cities animal husbandry development region, by economy by taking southern area as an example
Flourishing city is in occupation of huge livestock products consumption figure.With the development of economy, the beef and mutton of safe and high quality increasingly accounts for
According to the critical positions in people's diet.However, the production of high-quality beef and mutton be unable to do without good feed resource.Though southern area
So there is the advantage of huge consumption market, however, but lacking in conventional feed resource such as corn and soybean and sheep's hay, clover
Competitiveness.At the same time, there is Sweet potato germplasm abundant in China, and development potentiality is big.For ruminant, relatively
The feed of high microsteping is more suitable in nonruminant, if it is possible to the by-product production processing biology of Sweet Potato Industry chain be utilized to raise
Material carries out the deep processing and comprehensive utilization of Sweet Potato Industry chain by-product, develops the biology based on sweet potato starch industry by-product
Feed can make this " waste material " to become huge feed resource.Simultaneously according to the condition combination cattle and sheep physilogical characteristics of southern area,
The biological feedstuff based on sweet potato starch industry by-product is found out using mode, and is promoted, then to China's Sweet Potato Industry and
The sustainable and cooperative development important in inhibiting of animal husbandry.
Summary of the invention
The purpose of the present invention is to provide a kind of fermented type sweet potato dregs protein feeds, and crude protein content is high, bioavailability
It is higher, solve the problems, such as waste utilization.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of fermented type sweet potato dregs protein feed, the feed the preparation method is as follows:
A, the preparation of starch fiber degradation Hybrid NC machine tool liquid
The activation of candida utili,
Starch fiber mixed bacterium of degrading activates respectively, and starch fiber mixed bacterium of degrading includes: aspergillus niger, aspergillus oryzae, Trichoderma viride,
Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis and lactobacillus fermenti,
Aspergillus niger, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, ground
Starch fiber degradation Hybrid NC machine tool liquid is made with equal mass ratioes mixing in the activation culture liquid of clothing bacillus and lactobacillus fermenti；
The activation culture liquid of candida utili and the mixed bacterium of starch fiber degradation is mixed to get in the ratio of 2-3:1 mass
Mixed bacteria liquid is stand-by；
B, solid fermentation
Solid fermentation culture medium: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea
1-3%, pH value is naturally, surplus is water；
Fermented and cultured is carried out to the mixed bacteria liquid that step a is obtained using the solid fermentation culture medium, mixed bacteria liquid connects
Kind amount is 8-12%, is placed at 30 ± 2 DEG C of constant temperature, the room fermentation time 36h-48h of humidity 80-90%, layer thickness 5-
8cm obtains fermented type sweet potato dregs protein feed after fermentation.
Simulation rumen function production starch fiber of the present invention degrades mixed bacterium to improve the digestion to starch in substrate and fiber
Rate, in rumen fluid containing there are many can digestibility and utilization starch and bacterium, the fungi of cellulose etc., it is natural in inventor's simulation rumen liquid
Mixed bacterium obtained the starch fiber of suitable industrial-scale production and degraded mixed bacterium.
Candida utili bacterium is called Torula utilis Henneb or edible torula.The content of its protein and vitamin B all compares
Brewer's yeast is high, it can be using urea and nitric acid as nitrogen source, and not needing any growth factor is added in the medium can grow.
It can utilize pentose and hexose, can utilize the sulfite waste liquor of paper industry, moreover it is possible to utilize molasses, wood hydrolysis liquid etc.
Produce the edible protein of people and animals.Optimum fermentation temp is 25 DEG C~28 DEG C.
Fermentation sweet potato dregs crude protein content of feed of the invention is higher than the true egg of normal fermentation feed, especially fermented feed
Bai Hanliang is very high, accounts for the 92.8% of total nitrogen, therefore the bioavailability of the fermented feed is higher.Crude fat EE in fermented feed
Nutritional need of the content also close to ruminant.
Preferably, candida utili and starch fiber are degraded, the activation method of mixed bacterium is: 5-10 ° of B é brewer's wort, training
Support 28-30 DEG C of temperature, 120rpm shaking table culture 12h.
A kind of preparation method of fermented type sweet potato dregs protein feed, this method comprises the following steps:
A, the preparation of starch fiber degradation Hybrid NC machine tool liquid
The activation of candida utili,
Starch fiber mixed bacterium of degrading activates respectively, and starch fiber mixed bacterium of degrading includes: aspergillus niger, aspergillus oryzae, Trichoderma viride,
Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis and lactobacillus fermenti,
Aspergillus niger, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, ground
Starch fiber degradation Hybrid NC machine tool liquid is made with equal mass ratioes mixing in the activation culture liquid of clothing bacillus and lactobacillus fermenti；
The activation culture liquid of candida utili and the mixed bacterium of starch fiber degradation is mixed to get in the ratio of 2-3:1 mass
Mixed bacteria liquid is stand-by；
B, solid fermentation
Solid fermentation culture medium: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea
1-3%, pH value is naturally, surplus is water；
Fermented and cultured is carried out to the mixed bacteria liquid that step a is obtained using the solid fermentation culture medium, mixed bacteria liquid connects
Kind amount is 8-12%, is placed at 30 ± 2 DEG C of constant temperature, the room fermentation time 36h-48h of humidity 80-90%, layer thickness 5-
8cm obtains fermented type sweet potato dregs protein feed after fermentation.
A kind of sheep cultural method carried out using the fermented type sweet potato dregs protein feed, this method is: in sheep
It is cultivated in daily ration with the protein feed of sweet potato dregs protein feed substitution 30%-60%.Experiment proves that compared to being used alone
Rapeseed meal can be obtained in sheep daily ration with the protein feed of sweet potato dregs protein feed substitution 30%-60% as protein feed source
To ideal feeding effect, be conducive to sheep health, the production without influencing sheep.
A kind of sheep cultivates Diet Formula, the hair containing mass fraction 3%-10% in the sheep Diet Formula
Ferment type sweet potato dregs protein feed.
The beneficial effects of the present invention are: the present invention using sweet potato dregs as raw material, is mixed into mixed fermentation strain (with starch fiber point
Mixed bacterium is solved to mix with candida utili) ferment is made protein feed.In fermentation process, fermenting microbe can will be in sweet potato dregs
The much starch and fiber degradation contained holds digestible short-chain carbohydrates at animal, and fermenting microbe can also produce in degradation process
Raw a large amount of mycoproteins, further increase nutritive value.The ratio of fermentation step and fermenting microbe is adjusted, starch in raw material is improved
With the content of mycoprotein in the degradation rate and tunning of fiber, to promote the deep processing of sweet potato to utilize.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.It should be appreciated that this hair
Bright implementation is not limited by the following examples, and the accommodation in any form made to the present invention and/or changed will all be fallen
Enter the scope of the present invention.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, used equipment and raw material etc.
It is commercially available or commonly used in the art.Method in following embodiments is unless otherwise instructed the normal of this field
Rule method.
Sweet potato dregs: certain sweet potato processing factory is come from.
The candida utili of Chinese industrial Microbiological Culture Collection administrative center, deposit number: CICC 1314；
It includes: aspergillus niger, deposit number: CICC 2316 that starch fiber, which degrades and mixes bacterium,；Aspergillus oryzae, deposit number: CICC
2011；Trichoderma viride, deposit number: CICC 13038；Bacillus subtillis, deposit number: CICC 10089, lactic acid class brood cell
Bacillus, deposit number CICC 20133, bacillus amyloliquefaciens, deposit number: CICC 21746, trichoderma reesei, deposit number:
CICC 41027, bacillus licheniformis, deposit number: CICC 10182, lactobacillus fermenti, deposit number: CICC 22821.
Testing index
1, true albumen: test is using sweet potato dregs protein content after the measurement fermentation of Coomassie brilliant blue G250 method.Specific steps are such as
Under:
1) sample about 1g or so is weighed in 50ml centrifuge tube, 15ml0.9% physiological saline is added, and extraction is stood after oscillation
1h；
2) it is centrifuged after the completion of extraction, 2000min-1, 5min pipettes supernatant and sets 15ml centrifuge tube；
3) plus proper amount of active carbon is in the 15ml centrifuge tube for filling leaching liquor, stands 30min or more；
4)3000min-1, it is centrifuged 5min；
5) it takes supernatant 0.2ml to enter test tube to be measured, 6ml Coomassie brilliant blue stain is added, stand 5min or more, within 1h,
Visible spectrophotometer wavelength 595nm measures OD value；
6) true protein content is calculated.
2, crude protein: crude protein content in Kjeldahl nitrogen determination sample.
3, crude fat: utilizing Soxhlet fat extractor, and the fat and liposoluble substance in dissolved samples are in ether, difference
Calculate to obtain crude fat content.
4, NDF, ADF: using Fan Shi (Van Soest) washing fibre analysis method measurement neutral detergent fiber (NDF) and
Acid detergent fiber (ADF).
Embodiment 1:
1 mixed bacterium strain preparation
Starch fiber degradation Hybrid NC machine tool liquid preparation:
Starch fiber, which is degraded, mixes bacterium composition are as follows: aspergillus niger, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class brood cell
Bacillus, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis and lactobacillus fermenti.Will with 5 ° of B é brewer's worts, natural pH,
At 28-30 DEG C, the strain of 120rpm shaking table culture 12h activation is mixed with the mass ratio of 1:1:1:1:1:1:1:1:1, and shallow lake is made
Powder fiber degradation Hybrid NC machine tool liquid.
The activation of candida utili: 5 ° of B é brewer's worts, 121 DEG C sterilize 20 minutes, access strain, natural pH, culture temperature
28-30 DEG C of degree, 120rpm shaking table culture 12h is stand-by.
2 starch fibers, which are degraded, mixes bacterium and the evaluation and test of saccharomycete mixed fermentation fermentation parameter
2.1 experimental design
Test uses L9 (34) orthogonal arrage, it investigates fermentation time, inoculum concentration, urea additive amount, strain ratio and (it is false C: to produce protein
Silk yeast；R: starch fiber is degraded mixed bacterium) four influences of the factor to tunning true protein content.By the factor level of table 1
Combination carries out 3 repetitions and tests.
1 fermented by mixed bacterium parameter of table
2.2 fermented by mixed bacterium sweet potato dregs results
Former sweet potato dregs sample is compared, protein content is only 3.5%, crude fat 2.3%, 14.2% NDF's and 10.7%
ADF, nutritional ingredient is as shown in table 2 after starch fiber degradation mixes bacterium compound barm co-fermentation sweet potato dregs, and the content of true albumen has
Significantly increase, the decomposition amount of fibrous matter is about 30% or so in sweet potato dregs.Wherein the true protein content of processing group 6 and 8 is most
It is high to be higher than other processing groups significantly up to 28.52% and 28.07%, and the content of crude protein is processing group 6 and 8
28.79% and 28.98% is greater than other processing groups more significantly.And the incrementss of crude fat are average, are not noticeably greater than
The crude fat content of other processing groups.
The Nutrious fermented composition transfer index of 2 mixed bacteria sweet potato dregs of table
A is fermentation time.Under normal circumstances, substrate protein content extends at any time and increases in earlier fermentation, to one
After fixing time, reaches sufficiently fermentation saturation value, protein content reduction can be presented because of thallus self-dissolving later.Therefore, it is reasonable to grasp
Fermentation time be influence attenuation degree an important factor for.
B is the inoculum concentration of the mixed bacterium of starch fiber degradation.In general, suitable starch fiber degrade mixed bacterium ratio can, be
The proliferation of yeast provides better nutrition foundation.
C is urea additive amount.Urea is mentioned as the source N adding ingredient during bacterial multiplication early period for microbial reproduction
Material base is supplied.
D is strain ratio.C is yeast, and R is the mixed bacterium of starch fiber degradation, is added according to different ratios, in earlier fermentation
There may be different intermediate products.
In quadrature analysis table 3 it is found that according to very poor R size, the factor primary and secondary for influencing tunning true protein content is suitable
Sequence is fermentation time > inoculum concentration > urea additive amount > strain ratio, and according to the size of K value, four factors are most suitable for fermentation sweet potato dregs
Proportion should be A3B3C1D2, i.e. fermentation time 36h, inoculum concentration 12%, urea additive amount is 1%, and strain ratio is 2:1.
True albumen (TP) the content quadrature analysis of 3 mixed bacteria sweet potato dregs of table fermentation
In quadrature analysis table 4 it is found that according to very poor R size, the factor primary and secondary for influencing tunning crude protein content is suitable
Sequence is fermentation time > inoculum concentration > strain ratio > urea additive amount, and according to the size of K value, four factors are most suitable for fermentation sweet potato dregs
Proportion should be A3B2C1D2, i.e. fermentation time 36h, inoculum concentration 8%, urea additive amount is 1%, and strain ratio is 2:1.
Table 4 mixed bacteria sweet potato dregs ferment crude protein (CP) content quadrature analysis
Remarks: factor A, B, C, D respectively refer to fermentation time, strain inoculum concentration, urea additive amount and strain ratio.
To in the quadrature analysis table 5 for the effect for generating fat, it can be seen that by very poor size, the primary and secondary of influence factor is suitable
Sequence should be inoculum concentration > fermentation time > strain ratio > urea additive amount, and the most proper combination should be A2B2C3D3, i.e., fermentation time for 24 hours,
It is 8% that starch fiber, which degrades and mixes bacterium inoculum concentration, and urea addition 6%, strain ratio is 1:2.Illustrate that the mixed bacterium of starch fiber degradation exists
Possible role is bigger in terms of the production of EE.
5 mixed bacteria sweet potato dregs of table fermentation crude fat (EE) content quadrature analysis
Remarks: factor A, B, C, D respectively refer to fermentation time, strain inoculum concentration, urea additive amount and strain ratio.
To in the quadrature analysis table 6 and table 7 of cellulolytic effect, it can see by analytical table 6 and press very poor size, shadow
The primary and secondary sequence of the factor of sound should be inoculum concentration > fermentation time > urea additive amount > strain ratio, and then by R arrangement very poor in table 7
For strain ratio > urea additive amount > fermentation time > inoculum concentration.Compared to the ADF of 14.2% NDF and 10.7% as former state, decompose
Rate is 30% or so.
6 mixed bacteria sweet potato dregs of table fermentation neutral detergent fiber (NDF) content quadrature analysis
Remarks: factor A, B, C, D respectively refer to fermentation time, strain inoculum concentration, urea additive amount and strain ratio.
7 mixed bacteria sweet potato dregs of table fermentation acid detergent fiber (ADF) content quadrature analysis
This experiment mock bull fermentation organ cud, provides the composite bacteria that can decompose sweet potato dregs under aerobic conditions, is made
Liquid seeds have investigated fermentation time, inoculation for producing protein feed with the combined ferment sweet potato dregs of candida utili
Amount, influence of 4 factors of urea additive amount and strain adding proportion to zymotic efficiency produce the optimum condition fermentation of protein feed
Time 36h, inoculum concentration 12%, urea additive amount are 1%, and Yeast Cultivation liquid and starch fiber degradation Hybrid NC machine tool liquor ratio value are 2:
1。
Embodiment 2
A kind of fermented type sweet potato dregs protein feed, the preparation method is as follows:
The preparation of 1 starch fiber degradation Hybrid NC machine tool liquid
The activation of candida utili: 5 ° of B é brewer's worts, 121 DEG C sterilize 20 minutes, access strain, natural pH, culture temperature
28-30 DEG C of degree, 120rpm shaking table culture 12h is stand-by.
It includes: aspergillus niger, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class brood cell's bar that starch fiber, which degrades and mixes bacterium,
Bacterium, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis, lactobacillus fermenti.It will be with 5 ° of B é brewer's worts, natural pH, In
28-30 DEG C, the strain of 120rpm shaking table culture 12h activation is mixed with the mass ratio of 1:1:1:1:1:1:1:1:1, and starch is made
Fiber degradation Hybrid NC machine tool liquid.
Activated candida utili and starch fiber degradation Hybrid NC machine tool liquid are mixed to get in the ratio of 2:1 mass
Mixed bacteria liquid is stand-by.
2 solid fermentations
Solid fermentation culture medium: sweet potato dregs 40%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, urea 1%, pH value is certainly
So, surplus is water.
Fermented and cultured, the inoculum concentration of mixed bacteria liquid are carried out to the mixed bacteria liquid that step 1 obtains using solid fermentation culture medium
It is 12%, is placed at 30 DEG C of constant temperature, the room fermentation time 36h of humidity 90%, layer thickness 5-8cm.It obtains after fermentation
Fermented type sweet potato dregs protein feed.
3 feed nutrients
Pilot scale is carried out under this fermentation condition, sweet potato dregs protein feed nutritional ingredient is true protein 29 .7%, crude protein
32.0%, crude fat 4.5%, NDF9.7%, ADF8.5%, pH 4.5, lactic acid 89.0mmol/L.The fermentation sweet potato dreg fodder is thick
The true protein content that protein content is higher than normal fermentation feed, especially fermented feed is very high, accounts for the 92.8% of total nitrogen, therefore
The bioavailability of the fermented feed is higher.Nutritional need of the crude fat EE content also close to ruminant in fermented feed.In
Wet sweet potato dregs can directly be used when the factorial production, and (moisture of sweet potato processing byproduct sweet potato dregs is just close to of the invention
60%), entire manufacturing process is simple and quick, it is not necessary to as other fermented feeds need the drying course by raw material, and mixed bacterium
Lactic acid content is high in fermented feed, is conducive to the breeding for inhibiting the bad bacterium such as salmonella, Escherichia coli and improves the strong of animal
Health situation.
Embodiment 3
A kind of fermented type sweet potato dregs protein feed, preparation method is the same as embodiment 2, difference are as follows: solid fermentation culture medium:
Sweet potato dregs 45%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.5%, urea 2%, pH value is naturally, surplus is water.
Embodiment 4
A kind of fermented type sweet potato dregs protein feed, preparation method is the same as embodiment 2, difference are as follows: solid fermentation culture medium:
Sweet potato dregs 50%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.5%, urea 2.5%, pH value is naturally, surplus is water.
The application of embodiment sweet potato dregs protein feed
The sweet potato dregs protein feed that raw material uses above method pilot scale to obtain.
Test sets 4 processing daily rations: i.e. control group (C) feeds common daily ration (including sheep's hay and fine fodder)；Test 1,2,3
Group, respectively with the rapeseed meal of sweet potato dregs protein feed substitution 33%, 66% and 100%.Experimental basis daily ration and test daily ration are shown in Table
8。
Experimental animal selects the sheep of 60 dress health, carries out RANDOMIZED BLOCK DESIGN, connects approximately principle point by age in days, weight
At 4 groups, every group 15.Test sheep supply daily ration twice daily, free water.The test positive examination phase 30 days, pre-feeding period 7 days.Initially
Weight is 20 kilograms or so.
8 daily ration of table composition and its chemical component table
Sheep hurdle repairing, disinfection are carried out before trial prerun, inside and outside expelling parasite are carried out to all sheep, to reduce sheep hurdle ring
The influence of border health and helminth to sheep Nutrition and Metabolism.Unified number after random grouping, is fed in different sheds using by group.Raising
(9 points of every morning and at 4 points in afternoon) fixed special messenger's feeding, and sufficient clean drinking-water is provided in sheep hurdle.And guarantee that column home is good
Good health and epidemic prevention conditions and environment condition.Observation feeding, disease event are paid attention to during test.
The conventional nutrients content such as each processing group feed measurement dry matter of sampling analysis, crude protein, NDF, ADF.
Test is responsible for by special messenger, is responsible for experimental animal raising, Cheng Chong, record.Feed intake measures weekly.6:30 is empty in the morning
Abdomen weighing, measures feed intake, calculates separately each per day feed intake of processing group (ADFI), daily gain (ADG) and gain feed ratio
(G/F)。
Data useMIXED program analysis (SAS Institute, 2000) in software.Test result is with averagely
Several and SEM is indicated.
As can be seen from Table 9, each group sheep weight differences in on-test are not significant (P > 0.05).When in daily ration use sweet potato dregs egg
Feed intake can be improved when white feed substitution rapeseed meal and be up to 3.8%, but the addition of excessive sweet potato dregs protein feed may
It will affect feed intake, have the tendency that reducing feed intake.Increasing day by day for sheep can be improved using sweet potato dregs protein feed to weigh
2.6%.But the gain feed ratio difference of each group is not significant.
The sheep health status containing fermented feed of searching for food is significantly better than control group.When in daily ration add 6%-9% it is sweet
There is no diarrhea situations when potato residue protein feed.
9 sweet potato dregs protein feed of table substitutes influence of the rapeseed meal to sheep growth performance
Compared to rapeseed meal is used alone as protein feed source, substituted in sheep daily ration with sweet potato dregs protein feed
Ideal feeding effect can be obtained in the protein feed of 30%-60%, is conducive to sheep health, the production without influencing sheep.
Above-mentioned embodiment is only a preferred solution of the present invention, not the present invention is made in any form
Limitation, there are also other variations and modifications on the premise of not exceeding the technical scheme recorded in the claims.
Claims (5)
1. a kind of fermented type sweet potato dregs protein feed, it is characterised in that the feed the preparation method is as follows:
A, the preparation of starch fiber degradation Hybrid NC machine tool liquid
The activation of candida utili,
The mixed bacterium of starch fiber degradation activates respectively, and it includes: aspergillus niger, aspergillus oryzae, Trichoderma viride, withered grass that starch fiber, which degrades and mixes bacterium,
Bacillus, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis and lactobacillus fermenti；Black song
It is mould, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, lichens bud
Starch fiber degradation Hybrid NC machine tool liquid is made with equal mass ratioes mixing in the activation culture liquid of born of the same parents bacillus and lactobacillus fermenti；
The activation culture liquid of candida utili and the mixed bacterium of starch fiber degradation is mixed to get mixing in the ratio of 2-3:1 mass
Bacterium solution is stand-by；
B, solid fermentation
Solid fermentation culture medium: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea 1-3%, pH
Value is naturally, surplus is water；
Fermented and cultured, the inoculum concentration of mixed bacteria liquid are carried out to the mixed bacteria liquid that step a is obtained using the solid fermentation culture medium
It for 8-12%, is placed at 30 ± 2 DEG C of constant temperature, room fermentation time 36h-48h, the layer thickness 5-8cm of humidity 80-90%, ferments
Fermented type sweet potato dregs protein feed is obtained after the completion.
2. fermented type sweet potato dregs protein feed according to claim 1, it is characterised in that: candida utili and starch are fine
The activation method of the mixed bacterium of dimension degradation is: 5-10 ° of B é brewer's wort, 28-30 DEG C of cultivation temperature, 120rpm shaking table culture 12h.
3. a kind of preparation method of fermented type sweet potato dregs protein feed, it is characterised in that this method comprises the following steps:
A, the preparation of starch fiber degradation Hybrid NC machine tool liquid
The activation of candida utili,
The mixed bacterium of starch fiber degradation activates respectively, and it includes: aspergillus niger, aspergillus oryzae, Trichoderma viride, withered grass that starch fiber, which degrades and mixes bacterium,
Bacillus, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, bacillus licheniformis and lactobacillus fermenti, black song
It is mould, aspergillus oryzae, Trichoderma viride, Bacillus subtillis, lactic acid class bacillus, bacillus amyloliquefaciens, trichoderma reesei, lichens bud
Starch fiber degradation Hybrid NC machine tool liquid is made with equal mass ratioes mixing in the activation culture liquid of born of the same parents bacillus and lactobacillus fermenti；
The activation culture liquid of candida utili and the mixed bacterium of starch fiber degradation is mixed to get mixing in the ratio of 2-3:1 mass
Bacterium solution is stand-by；
B, solid fermentation
Solid fermentation culture medium: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea 1-3%, pH
Value is naturally, surplus is water；
Fermented and cultured, the inoculum concentration of mixed bacteria liquid are carried out to the mixed bacteria liquid that step a is obtained using the solid fermentation culture medium
It for 8-12%, is placed at 30 ± 2 DEG C of constant temperature, room fermentation time 36h-48h, the layer thickness 5-8cm of humidity 80-90%, ferments
Fermented type sweet potato dregs protein feed is obtained after the completion.
4. a kind of sheep cultural method carried out using fermented type sweet potato dregs protein feed described in claim 1, feature are existed
In: in sheep daily ration with sweet potato dregs protein feed substitution 30%-60% protein feed cultivated.
5. a kind of sheep cultivates Diet Formula, it is characterised in that: contain mass fraction 3%-10%'s in the sheep Diet Formula
Fermented type sweet potato dregs protein feed described in claim 1.
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