CN103749967A - Sweet potato residue fermentation feed and preparation method thereof - Google Patents
Sweet potato residue fermentation feed and preparation method thereof Download PDFInfo
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- CN103749967A CN103749967A CN201410039421.5A CN201410039421A CN103749967A CN 103749967 A CN103749967 A CN 103749967A CN 201410039421 A CN201410039421 A CN 201410039421A CN 103749967 A CN103749967 A CN 103749967A
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Abstract
The invention discloses a sweet potato residue fermentation feed and a preparation method thereof,and relate to the field of preparing animal feed from plant materials. The fermentation feed is prepared by using 75-80% of sweet potato residue and 20-25% of wheat bran as a raw material and an auxiliary material, and adding a reagent based on the raw material; mixing 0.75-1.5% of urea, 0.75-1.5% of ammonium sulfate, 0.5-0.7% of mono potassium phosphate and 0.4-0.6% of magnesium sulfate to obtain a fermentation substrate; mixing bacillus subtilis, saccharomycopsis fibuligera, trichoderma viride and bacterium solution of lactobacillus plantarum to prepare a bacteria liquid of combined fermentation strains through aerobic fermentation. The weight ratio of the fermentation substrate to the bacteria liquid of the combined fermentation strains is 1:1-1.2; the volume ratio of four bacteria liquids is 1-3:1-3:1-3:1-3. The method can increase the amount of true protein of the fermentation substrate, reduce the amount of coarse fibers, improve the feed palatability and digestibility and utilization, and fully improve the nutritional value of sweet potato residue, thereby having both economic benefits and environmental benefits.
Description
Technical field
The technical field of animal that the present invention relates to make from vegetable material.
Background technology
China is maximum in the world sweet potato producing country, and sweet potato cultivated area is 7500～8,000 ten thousand mu throughout the year, accounts for 4.2% of China total cultivated area, accounts for the cultivated area of 60% left and right, the world, and output accounts for total 80% left and right of producing, the world.Sweet potato dregs is the residue producing in sweet potato starch process, its water content is high, it is many certainly to carry disease germs, easily putrid and deteriorated, in dry, protein content is low, fiber content is high, conventionally be taken as that discarded object abandons or simply utilize as low-quality feedstuff, cause the waste of resource and the pollution of environment, how development and utilization sweet potato dregs resource has become current China's starch industry difficult problem in the urgent need to address.By biotechnology, particularly microbial fermentation technology is processed feedstuff, is improved nutritive value, is the safe and efficient method of low-quality feed resource exploitation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of sweet potato dregs fermented feed and preparation method thereof, can increase the true protein content of fermentation substrate, reduce crude fibre amount, improve feed palatability and digestive utilization ratio, improve sweet potato dregs nutritive value comprehensively, there is economy and environment double benefit.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of sweet potato dregs fermented feed, take potato dregs 75-80wt% and wheat bran 20-25wt% is supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, bacterium liquid with bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is mixed into combined fermentation bacterial classification bacterium liquid, and aerobic fermentation is prepared from; Water content≤the 14wt% of described potato dregs; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; The bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 * 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
The ratio that preferred combined fermentation bacterial classification bacterium liquid is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
The preparation method of sweet potato dregs fermented feed, comprises the following steps:
(1) take potato dregs 75-80wt% and wheat bran 20-25wt% is supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato dregs.
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, fermentation substrate evenly mixes by the weight ratio of 1:1-1.2 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48-72 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 * 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
The ratio that preferred combined fermentation bacterial classification bacterium liquid is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
Bacillus subtilis (Bacillus subtilis) 10075, is purchased from Chinese industrial microorganism fungus kind preservation administrative center; Saccharomycopsis fibuligera (Endomycopsis fibuligera) 20154, Trichoderma viride (Trichoderma viride) 30498, Lactobacillus plantarum (Lactobacillus plantarum) 11016, be purchased from Chinese agriculture microorganism fungus kind preservation administrative center.
The preparation of bacillus subtilis used, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum bacterium liquid can adopt prior art, the invention provides following methods.
1, the preparation of Lactobacillus plantarum bacterium liquid
The Lactobacillus plantarum that 4 ℃ of inclined-planes are preserved is connected on MRS liquid tube culture medium and activates, at 37 ℃ with 220rmin
-1shaken cultivation 24h, cultivates seed as one-level; With 1% inoculum concentration, be connected in MRS triangular flask fluid nutrient medium again, at 37 ℃ with 220rmin
-1shaken cultivation 24h.MRS culture medium: peptone 10 g, beef extract 10 g, yeast extract 5 g, hydrogen citrate 2 g, glucose 20 g, Tween 80 1 ml, sodium acetate 5 g, dipotassium hydrogen phosphate 2 g, magnesium sulfate 0.58 g, manganese sulfate 0.25 g, distilled water 1000 ml, 6.2～6.6,121 ℃ of moist heat sterilization 15min of pH.
2, the preparation of saccharomycopsis fibuligera bacterium bacterium liquid
The saccharomycopsis fibuligera bacterium that 4 ℃ of inclined-planes are preserved is connected on YEPD liquid tube culture medium and activates, at 30 ℃ with 220rmin
-1shaken cultivation 24h, cultivates seed as one-level; With 1% inoculum concentration, be connected in YEPD triangular flask fluid nutrient medium again, at 30 ℃ with 220rmin
-1shaken cultivation 24h.YEPD culture medium: dusty yeast 10 g, peptone 20 g, glucose 20 g, distilled water 1000 ml, pH6.0,115 ℃ of moist heat sterilization 20min.
3, the preparation of bacillus subtilis bacterium liquid
The bacillus subtilis that 4 ℃ of inclined-planes are preserved is connected on beef extract-peptone liquid tube culture medium and activates, at 32 ℃ with 220rmin
-1shaken cultivation is cultivated 24h, as one-level, cultivates seed; With 1% inoculum concentration, be connected in beef extract-peptone triangular flask fluid nutrient medium again, at 32 ℃ with 220rmin
-1shaken cultivation 24h.Beef-protein medium: beef extract 0.5 g, peptone 15 g, sodium chloride 5 g, glucose 20 g, distilled water 1000 ml, 7.0～7.2,115 ℃ of moist heat sterilization 20min of pH.
4, the preparation of trichoderma viride liquid
The Trichoderma viride that 4 ℃ of inclined-planes are preserved is connected on Cha Shi liquid tube culture medium and activates, at 28 ℃ with 220rmin
-1shaken cultivation is cultivated 36h, as one-level, cultivates seed; With 1% inoculum concentration, be connected in Cha Shi triangular flask fluid nutrient medium again, at 28 ℃ with 220rmin
-1shaken cultivation 36h.Czapek's medium: sodium nitrate 3g, dipotassium hydrogen phosphate 1 g, magnesium sulfate 0.5 g, potassium chloride 0.5 g, ferrous sulfate 0.01 g, sucrose 30 g, distilled water 1000 ml, pH nature, 121 ℃ of moist heat sterilization 20min.
The present invention is mainly that application potato dregs is raw material, wheat bran is auxiliary material, add on this basis reagent: urea, ammonium sulfate, potassium dihydrogen phosphate and magnesium sulfate, be mixed to get fermentation substrate, the combined fermentation bacterial classification bacterium liquid being mixed into through the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum bacterium liquid, aerobic fermentation is prepared from.Its principle is: potato dregs and wheat bran contain enough starch and crude fibre and a small amount of true albumen; Urea, ammonium sulfate contain the available a large amount of inorganic nitrogens of microorganism; Magnesium sulfate, potassium dihydrogen phosphate can promote some beneficial microbe growth; Above-mentioned three class material action compensatings, application simultaneously can produce Overlay.
Application saccharomycopsis fibuligera can improve fermentation substrate true protein content; Bacillus subtilis can produce protease, improves protein utilization rate; Trichoderma viride can eccrine fiber element enzyme degradation of fibers; Lactobacillus plantarum can produce lactic acid, improves feed local flavor; Fermentation substrate is after aerobic fermentation is processed, and true protein content is 17.61%, and total amount has improved 44.30%, and crude fiber content is 26.60%, and total amount has reduced by 26.60%, and has sweet-smelling smell.
The beneficial effect that adopts technique scheme to produce is:
(1) the present invention can increase the true protein content of fermentation substrate, reduces crude fibre amount, improves feed palatability and digestive utilization ratio, improves sweet potato dregs nutritive value comprehensively, has economy and environment double benefit; After application the inventive method is processed fermentation substrate, true protein content is 17.61%, and total amount has improved 44.30%, and crude fiber content is 26.60%, and total amount has reduced by 26.60%, and has sweet-smelling smell.
(2) product of the present invention substitutes wheat bran by 15% part by weight, can improve growing and fattening pig feed intake, daily gain, reduces feedstuff-meat ratio.
(3) the present invention is pure green product, without any side effects, does not produce incompatibility with other raw materials.
The specific embodiment
Potato dregs is purchased from Qinhuangdao Gao Cheng food industries Co., Ltd, water content≤14wt%, and market is on sale.
Embodiment 1
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato dregs, 40 grams of wheat brans, 3 grams, urea, 3 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: after above-mentioned supplementary material and reagent are weighed, input pulverizer is pulverized, dropping into mixer mixes again, make fermentation substrate, then the bacterium liquid of bacillus subtilis, button capsule yeast, Trichoderma viride and Lactobacillus plantarum of take is combined fermentation bacterial classification bacterium liquid, and wherein the bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 * 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes, fermentation substrate evenly mixes by the weight ratio of 1:1 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48 hours, dry to water content≤14wt% for 70-80 ℃, pulverize.
Embodiment 2
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato dregs, 40 grams of wheat brans, 3 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:1:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.2 with combined fermentation bacterial classification bacterium liquid.
Embodiment 3
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato dregs, 50 grams of wheat brans, 3 grams, urea, 3 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:2:2:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.1 with combined fermentation bacterial classification bacterium liquid.
Embodiment 4
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato dregs, 50 grams of wheat brans, 3 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 1:3:3:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.05 with combined fermentation bacterial classification bacterium liquid.
Embodiment 5
Adopt supplementary material and the reagent of following weight composition: 160 grams of potato dregs, 40 grams of wheat brans, 1.5 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:1:2:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.15 with combined fermentation bacterial classification bacterium liquid.
Embodiment 6
Adopt supplementary material and the reagent of following weight composition: 150 grams of potato dregs, 50 grams of wheat brans, 1.5 grams, urea, 1.5 grams, ammonium sulfate, 1.2 grams of potassium dihydrogen phosphates, 1 gram, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:2:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
Embodiment 7
Adopt supplementary material and the reagent of following weight composition: 156 grams of sweet potato dregs, 44 grams of wheat brans, 2 grams, urea, 2.5 grams, ammonium sulfate, 1 gram of potassium dihydrogen phosphate, 1.2 grams, magnesium sulfate.
Preparation method: be that with the difference of embodiment 1 ratio that combined fermentation bacterial classification bacterium liquid is 2:3:1:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
Embodiment 8
Adopt supplementary material and the reagent of following weight composition: 152 grams of sweet potato dregs, 48 grams of wheat brans, 2.5 grams, urea, 2 grams, ammonium sulfate, 1.4 grams of potassium dihydrogen phosphates, 0.8 gram, magnesium sulfate.
Preparation method: the ratio that is 3:1:3:2 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio with the difference combination fermented bacterium bacterium liquid of embodiment 1 mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.2 with combined fermentation bacterial classification bacterium liquid.
Embodiment 9
Adopt supplementary material and the reagent of following weight composition: 154 grams of sweet potato dregs, 46 grams of wheat brans, 1.7 grams, urea, 1.8 grams, ammonium sulfate, 1.3 grams of potassium dihydrogen phosphates, 0.9 gram, magnesium sulfate.
Preparation method: the ratio that is 3:2:1:3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio with the difference combination fermented bacterium bacterium liquid of embodiment 1 mixes; Fermentation substrate evenly mixes by the weight ratio of 1:1.1 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 72 hours.
Embodiment 10
Adopt supplementary material and the reagent of following weight composition: 158 grams of sweet potato dregs, 42 grams of wheat brans, 2.2 grams, urea, 2.7 grams, ammonium sulfate, 1.1 grams of potassium dihydrogen phosphates, 1.1 grams, magnesium sulfate.
Preparation method: the ratio that is 3:3:2:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio with the difference combination fermented bacterium bacterium liquid of embodiment 1 mixes, 28-30 ℃ of aerobic fermentation 60 hours.
Its using method of sweet potato dregs fermented feed of the present invention is: with this product, at growing-finishing pigs diets moderate, substitute 15% wheat bran, other raw materials are constant.
Experimental example:
This sweet potato dregs fermented feed affects experimental design to growing-finishing pig production performance
1.1 experimental animals and grouping
Select age in days to differ to be no more than 120 of 30 kilograms of identical left and right pigs of 7 days, kind, the principle identical according to genetic background, body weight is close, sex ratio is consistent is divided into 2 processing at random, 6 repetitions are established in every processing, 10 of every repetitions, and between each group, starting weight difference is not remarkable.
Control group: the basal diet of feeding; Test group: feed and substitute the test daily ration of equivalent wheat bran with 15% sweet potato dregs fermented feed.
1.2 test daily ration and Treatment Design
With reference to NRC(1998) growing-finishing pig nutrition requirements in conjunction with production practices preparations basal diet.
Table 1 Diet Formula and trophic level
1.3 feeding and management
Test is carried out in same closed pig house, raises on the net, and humiture is suitable; Free choice feeding and drinking-water.Immunity disinfectant program is undertaken by pig farm conventional method.Test is responsible for by special messenger, in strict accordance with test requirements document, manages.Feeding and management is carried out with reference to the feeding and management rules of this product boar.Test period 40 days.
1.4. test method
The individuality of take when on-test and end is weighed as unit carries out empty stomach to all test pig, records in detail body weight; In process of the test, take and repeat to record in detail feed intake as unit; Record in detail pig death and superseded situation, while there is dead naughty pig, expect and weigh for timely section.
1.5 detect index
The average daily gain of growth performance index, average daily ingestion amount and feedstuff-meat ratio.
1.6 data processings and statistical analysis technique
Data acquisition carries out variance analysis with the ANOVA in SPSS13.0 statistical software; Pat everywhere between mean and carry out significance of difference comparison by Duncan method, result of the test data represent with mean+SD.
2. results and analysis
The impact of table 2 on growing-finishing pig production performance
As can be seen from Table 2, test group off-test weight ratio control group has improved 2.62%(P>0.05); Average daily gain has improved 5.60%(P>0.05 than control group); Average daily ingestion amount has improved 0.55%(P>0.05 than control group); Feedstuff-meat ratio reduces 4.85%(P>0.05 than control group), although difference is not remarkable, be all improved trend.
3. conclusion
By result of the test, can be obtained to draw a conclusion:
In growing and fattening pig feed, with sweet potato dregs fermented feed equivalent substitution wheat bran, can improve growing and fattening pig feed intake, daily gain, reduce feedstuff-meat ratio;
Because this product is safe green feedstuff, and cost is significantly lower than substitute-wheat bran, so produce market has a extensive future.
Claims (4)
1. a sweet potato dregs fermented feed, it is characterized in that: take potato dregs 75-80wt% and wheat bran 20-25wt% is supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, be mixed to get fermentation substrate, bacterium liquid with bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is mixed into combined fermentation bacterial classification bacterium liquid, and aerobic fermentation is prepared from; Water content≤the 14wt% of described potato dregs; The weight ratio of fermentation substrate and combined fermentation bacterial classification bacterium liquid is 1:1-1.2; The bacterial concentration of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 * 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
2. sweet potato dregs fermented feed according to claim 1, is characterized in that the ratio that described combined fermentation bacterial classification bacterium liquid is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
3. a preparation method for sweet potato dregs fermented feed as claimed in claim 1 or 2, is characterized in that, comprises the following steps:
(1) take potato dregs 75-80wt% and wheat bran 20-25wt% is supplementary material, on this supplementary material basis, add reagent: urea 0.75-1.5wt%, ammonium sulfate 0.75-1.5wt%, potassium dihydrogen phosphate 0.5-0.7wt% and magnesium sulfate 0.4-0.6wt%, mix, make fermentation substrate; Water content≤the 14wt% of described potato dregs;
(2) above-mentioned fermentation substrate is mixed into combined fermentation bacterial classification bacterium liquid with the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum, fermentation substrate evenly mixes by the weight ratio of 1:1-1.2 with combined fermentation bacterial classification bacterium liquid, 28-30 ℃ of aerobic fermentation 48-72 hour, dries and pulverizes; The bacterial concentration of described bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum is 1 * 10
6individual/ml, the ratio that combined fermentation bacterial classification bacterium liquid is 1-3:1-3:1-3:1-3 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
4. the preparation method of sweet potato dregs fermented feed according to claim 3, is characterized in that the ratio that described combined fermentation bacterial classification bacterium liquid is 2:2:3:1 by the bacterium liquid of bacillus subtilis, saccharomycopsis fibuligera, Trichoderma viride and Lactobacillus plantarum according to volume ratio mixes.
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| CN107119005A (en) * | 2017-05-11 | 2017-09-01 | 张维秀 | A kind of bafillus natto enhancer of proliferation and preparation method thereof |
| CN110050881A (en) * | 2019-03-24 | 2019-07-26 | 张学海 | A kind of process of probiotic feed solid state fermentation |
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