CN106173377A - The sweet potato dregs fermented feed of a kind of ruminant and application thereof - Google Patents
The sweet potato dregs fermented feed of a kind of ruminant and application thereof Download PDFInfo
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Abstract
The present invention relates to a kind of feedstuff, particularly to sweet potato dregs fermented feed and the application thereof of a kind of ruminant.The sweet potato dregs fermented feed of a kind of ruminant, described fermented feed is that inoculation rumen fluid after fermentation 24h is derived above in solid-state fermentation culture medium, and rumen fluid inoculum concentration is 7 10%；Described solid-state fermentation culture medium includes the following component in terms of mass fraction: sweet potato dregs 40 50%, potassium dihydrogen phosphate 0.5 0.8%, magnesium sulfate 0.3 0.5%, carbamide 1% ~ 3%, surplus is water.Utilize rumen fluid culture technique, high protein feed both can be provided, also solved the problem that problem of environmental pollution.
Description
Technical field
The present invention relates to a kind of feedstuff, particularly to sweet potato dregs fermented feed and the application thereof of a kind of ruminant.
Background technology
Along with popularizing of livestock technology, China's aquaculture development is rapid.But, China's meat per capita, egg, milk intake with
Still there is the consumption figure of high-grade livestock products such as bigger gap, particularly beef and mutton etc. in developed country well below developed country.With send out
Reaching country to compare, current China aquaculture Rearing facility, environmental condition are poor, and culture diseases is the most, and maximum of which
Problem is exactly feed resource shortage, causes aquaculture cost height to look forward to, and household income is on the low side.Meanwhile, China's grain there is also sternly
The deficiency of weight, energy feed and the albumen feedstuff of annual import increase year by year.Therefore, by feeding animals mankind's edible corn
Increase the road not science of herding yield.The most preferably developing ecology grain-saving type ruminant cultivation is that China's animal husbandry can
The only way which must be passed of sustainable development.
Rhizoma Dioscoreae esculentae is as one of China cereal crops, and cultivated area accounts for global 70%, annual production more than 11.7 tons,
Accounting for the 80% of whole world total output, cultivated area and yield all occupy the first in the world.Sweet potato dregs is that Rhizoma Dioscoreae esculentae produces starch and vermicelli
During by-product, it is big that fresh sweet potato slag has water content, is difficult to store, the feature such as easily rot smelly, most factory
Family is regarded waste material and is abandoned, and not only causes the waste of resource, goes back serious environment pollution.Now potato slag utilize this mainly to pass through
Following approach: (1), fresh potato slag sealing and fermenting, as fresh feed.(2), dry, pulverize, make mixed feed, feed pigs with little casual household again
It is main.(3), for breeding famous and precious edible fungi such as Lentinus Edodes and Pleurotus ostreatus etc..(4), wine brewing etc..(5), make above-mentioned several the approach such as fuel.Sweet
The characteristic of potato slag high microsteping height starch causes its Direct-fed animal in aquaculture to hinder growth of animal.Except containing a small amount of starch
Outward, major part is cellulose and hemicellulose, and protein content is the lowest, and Direct-fed animal digestion is very poor.Additionally, sweet potato dregs
Containing the antinutritional factor such as cellulose, trypsin ihhibitor, affect animal intestinal and nutrient substance is absorbed.How
Development and utilization sweet potato dregs resource is the study hotspot that more current scholars are main.
Fermented feed refers under manual control condition, and the microorganism metabolic activity by self, by vegetalitas, animality
With in mineral material antinutritional factor decompose or convert, produce more can by domestic animal search for food digestion, absorption nutrient higher and
The feedstuff of nonhazardous effect.Sweet potato dregs nutritive value can be improved, by low quality albumen in sweet potato dregs by fermentable
It is converted into high-quality tropina, improves albumen quality；Microorganism simultaneously grows in sweet potato dregs fermentation substrate, can degrade anti-
Trophic factors, secretes benefit materials, is favorably improved the animal digestive utilization ratio to feedstuff.
Utilize rumen fluid culture technique, high protein feed both can be provided, also solved the problem that problem of environmental pollution.
Summary of the invention
The present invention provides the sweet potato dregs fermented feed of a kind of ruminant, this feed conversion rumen fluid fermenting and producing, both may be used
High protein feed is provided, also solves the problem that problem of environmental pollution.
The technical solution adopted for the present invention to solve the technical problems is:
The sweet potato dregs fermented feed of a kind of ruminant, described fermented feed is inoculation cud in solid-state fermentation culture medium
After fermentation 24h is derived above for liquid, and rumen fluid inoculum concentration is 7-10%；Described solid-state fermentation culture medium includes with mass fraction
The following component of meter: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, carbamide 1%～3%,
Surplus is water.
The preparation method of the sweet potato dregs fermented feed of a kind of described ruminant, in described solid-state fermentation culture medium
Each raw material is dissolved in suitable quantity of water the most respectively, is mixed in proportion after being sterilized separately, after supplying the water of formula ratio, after inoculating impurity screening
Rumen fluid, in constant incubator 30 DEG C cultivate more than 24h.The fermentation process used is aerobic solids fermentation, it is not necessary to detest
The equipment of aerobe fermentation and strict control condition, simple.As preferably, described incubation time is 24-26h.
The application in sheep or beef cattle feed of a kind of described sweet potato dregs fermented feed, it is characterised in that: described Rhizoma Dioscoreae esculentae
Residue fermented feed consumption can account for the 6%-15% of sheep or cattle diet.
A kind of sheep feeds Diet Formula, and this formula includes the component of following mass fraction:
Leymus chinensis (Trin.) Tzvel. 40-50%,
Grass 17-20%,
Semen Maydis 16-18%,
Bean cake 5-6%,
Wheat bran 3-4%,
Sal 0.5-0.8%,
Calcium hydrogen phosphate 0.5-0.8%,
Sweet potato dregs fermented feed 6-15%.
A kind of beef cattle feeds Diet Formula, and this formula includes the component of following mass fraction:
Leymus chinensis (Trin.) Tzvel. 20-30%,
Silagecorn 18-38%,
Semen Maydis 24-26%,
Bean cake 6-8%,
Wheat bran 4-6%,
Sal 0.5-0.8%,
Calcium hydrogen phosphate 0.5-0.8%,
Sweet potato dregs fermented feed 6-15%.
The cud of ruminant is a huge anaerobic fermentation tank, in rumen fluid containing multiple can digestibility and utilization starch and
The antibacterial of cellulose, fungus, protozoon etc..Rumen fluid is to take out in ruminant tumor gastric, takes by installing rumen fistula to cattle
Rumen fluid.Present invention sweet potato dregs fermented feed substitutes high-quality protein feed, and cost is substantially reduced.
The invention has the beneficial effects as follows: China's Rhizoma Dioscoreae esculentae cultivated area has 9,300,000 hectares, total output, at about 4.5 hundred million tons, accounts for
The 80% of Gross World Product, therefore sweet potato dregs yield is huge.Because using rumen fluid to send out as culture, the sweet potato dregs produced
Ferment feedstuff closer to the rumen zymosis product of cattle body itself, well can be absorbed by cattle body compared with other fermented feed,
True protein content reaches 30%.And some other fermentation roughage protein content mostly at 10%-20%.To cattle, fistula can be installed
Pipe takes rumen fluid, it is also possible to utilize the rumen fluid in slaughterhouse, it is to avoid the waste of precious resources.Additionally this technology directly uses solid
Fermentation process, unlike EM fermented feed etc. needs anaerobic fermentation and relevant device, and fermentation time also compares EM fermentation
5-6 days of feedstuff short.
Detailed description of the invention
Below by specific embodiment, technical scheme is described in further detail.Should be appreciated that this
Bright enforcement is not limited to the following examples, and any pro forma accommodation and/or the change of being made the present invention all will fall
Enter scope.
In the present invention, if not refering in particular to, all of part, percentage ratio are unit of weight, the equipment used and raw material etc.
All it is commercially available or commonly used in the art.Method in following embodiment, if no special instructions, is the normal of this area
Rule method.
Sweet potato dregs: from certain Rhizoma Dioscoreae esculentae processing factory.Rumen fluid is to take out in ruminant tumor gastric, by installing cud to cattle
Fistula takes rumen fluid.
Embodiment 1:
Solid-state fermentation culture medium: sweet potato dregs 40%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, carbamide 1%～6%, remaining
Amount is water.Sweet potato dregs is ground into particle diameter in advance in the range of 0.5-2mm.
It is three influence factors with fermentation time, inoculum concentration, carbamide addition, three levels, arrange 9 by orthogonal test
Process group, each process group 3 repetition.
In solid-state fermentation culture medium, sweet potato dregs ratio accounts for 40%.Sweet potato dregs 60% cannot be carried out autoclaving after being watered, because of
Being 121 DEG C, after 30min, Rhizoma Dioscoreae esculentae is stuck with paste in puffy, it is impossible to pour flat board into.Therefore sweet potato dregs and additive (carbamide, magnesium sulfate, phosphoric acid
Potassium dihydrogen) aqueous solution is sterilized separately, after on superclean bench, culture dish mixes in proportion, inoculates into by 4 layers of yarn
Rumen fluid after cloth impurity screening.In constant incubator, cultivate 12h, 24h, 36h for 30 DEG C.
Table 1 cud direct inoculation fermentation parameter
Concrete operations are: see table 1, will load in 9cm culture dish about solid-state fermentation culture medium 5g, 121 DEG C of sterilizings
After 20min, accessing rumen fluid by the 4% of fermentation culture basic weight, 8%, 12% (v/w) in every box, inoculation back box mouth covers two-layer
Sterilizing wet gauze, 30 DEG C cultivate 12,24,36h, through 65 DEG C of drying, pulverize after for analysis mensuration.
Testing index
A, true albumen: test uses Coomassie brilliant blue G250 method to measure sweet potato dregs protein content after fermentation.Concrete steps are such as
Under:
1) weigh sample about about 1g in 50ml centrifuge tube, add 15ml0.9% normal saline, after vibration, stand extraction
1h；
2) it is centrifuged after having extracted, 2000min-1, 5min, pipette supernatant and put 15ml centrifuge tube；
3) add proper amount of active carbon in the 15ml centrifuge tube filling lixiviating solution, stand more than 30min；
4)3000min-1, centrifugal 5min；
5) taking supernatant 0.2ml, to enter test tube to be measured, adds 6ml Coomassie brilliant blue stain, stands more than 5min, within 1h,
Visible spectrophotometer wavelength 595nm measures OD value；
6) true protein content is calculated.
B, crude fat: utilize Soxhlet fat extractor, fat in dissolved samples and liposoluble substance in ether, difference
Calculate crude fat content.
Interpretation of result
In former sweet potato dregs sample, protein content is only 3.5%, and crude fat 2.3%, by strain in rumen fluid to sweet potato dregs
The fermentation of culture medium, it can be seen that the content of albumen is significantly increased, though because being added with carbamide in culture medium and make slightly
The mensuration of the content of albumen has an interference, but can measure containing of true albumen in sample clearly by Coomassie brilliant blue G250 method
Amount.Improve significantly from Table 2, it can be seen that the content of albumen has had, can reach 29.63% and 29.16%, significantly
The protein content organized higher than other.And the content of crude fat there has also been and significantly increases, the highest have 5.32%, is significantly higher than it
His partly process group, illustrates relatively to be suitable under this process the generation of fat.
As shown in table 3, A is fermentation time.Generally, in earlier fermentation substrate protein white matter content extend in time and
Increasing, after certain time, reach fully to ferment saturation value, can present protein content afterwards because of thalline self-dissolving and reduce.Therefore,
Grasping rational fermentation time is the key factor affecting attenuation degree.B is rumen fluid inoculum concentration.In general, early stage inoculation
More rumen fluid, first gives more bacterial content, and secondly, rumen fluid is also for transition one better suited PH of offer of strain
And material base.C is carbamide addition.Carbamide is as N source adding ingredient, during early stage bacterial multiplication, numerous for microorganism
Grow and provide material base.
Table 2 rumen fluid sweet potato dregs Nutrious fermented composition transfer index
Table 3 rumen fluid direct inoculation sweet potato dregs fermentation true protein content quadrature analysis
Remarks: factor A, B, C refer to fermentation time, rumen fluid inoculum concentration and carbamide addition respectively.
As shown in table 3, be combined as ferment 24h, rumen fluid 12%, carbamide add 1% or fermentation 36h, rumen fluid 8%,
When carbamide adds 1%, fermentation afterproduct true protein content is higher, respectively reaches 29.63% and 29.16%.
Statistical analysis understands, and according to the size of extreme difference R, the factor primary and secondary order affecting tunning true protein content is urine
Element addition > fermentation time > inoculum concentration, according to the size of K value, three factors are best suitable for the proportioning of fermentation sweet potato dregs and should be
A2B2C1, i.e. fermentation time 24h, inoculum concentration 8%, carbamide addition is 1%.
To in the quadrature analysis table 4 of tunning crude fat content, it can be seen that by pole extent, the master of influence factor
Secondary order should be carbamide addition > inoculum concentration > fermentation time, the most proper combination should be A2B2C1, i.e. fermentation time 24h, rumen fluid
Inoculum concentration is 8%, and carbamide adds 1%.
Table 4 rumen fluid direct inoculation sweet potato dregs fermentation crude fat content quadrature analysis
Remarks: factor A, B, C refer to fermentation time, rumen fluid inoculum concentration and carbamide addition respectively.
To sum up, during with the mixing rumen microorganism in rumen fluid as strain fermentation sweet potato dregs, in general, with true albumen
If paying the utmost attention to crude fat index, the most proper combination is fermentation time 24h, and rumen fluid inoculum concentration is 8%, and carbamide adds 1%.
And rumen fluid to come from ruminant comparatively safe for feeding ruminants.
The application in sheep feeds of the sweet potato dregs fermented feed of the embodiment present invention
1 MATERIALS METHODS
1.1 test material
By fermentation time 24h, rumen fluid inoculum concentration is 8%, and carbamide adds the method for 1% and makes fermented feed.Obtain
The true albumen of sweet potato dregs fermented feed is 29.8%, and crude fat is 4.47.
1.2 EXPERIMENTAL DESIGN
Test and set 2 process daily rations: be i.e. matched group (C), feed common daily ration (including Leymus chinensis (Trin.) Tzvel. and concentrate)；Fermented feed group
(RF), with fermented feed Substitute For Partial Semen Maydis and rapeseed cake.Experimental basis daily ration and test daily ration are shown in Table 5.
Experimental animal selects the sheep that 60 dresses are healthy, carries out matched-pair design, is divided into 2 by age in days, the body weight principle that is close
Group, often group 30.Test sheep supply daily ration every day twice, freely drink water.Test is just trying the phase 30 days, raises the phase in advance 7 days.Original body mass
It it is about 20 kilograms.
Table 5 daily ration composition and chemical component table thereof
1.3 trial
Carry out sheep hurdle repairing, sterilization before prerun, all sheep are carried out inside and outside anthelmintic, to reduce sheep hurdle environmental health and to post
The infested impact on sheep Nutrition and Metabolism.Unified number after random packet, uses and raises by component hurdle.Raise (every morning 9 point
With lower 4 points) fixing special messenger feeds, and the sufficient clean drinking-water of offer in sheep hurdle.And ensure the health and epidemic prevention bar that column home is good
Part and environmental condition.During test note observation search for food, disease event.
2 measure project and method
2.1 sample analysis
Each process group feedstuff measures the conventional nutrients content such as dry, crude protein.
2.2 growth performance
Test is responsible for by special messenger, is responsible for experimental animal raising, Cheng Chong, record.
Feed intake measures weekly.6:30 weighs on an empty stomach in the morning, measures feed intake, calculates that each process group is per day adopts respectively
Appetite (ADFI), daily gain (ADG) and gain feed ratio (G/F).
The collection of 2.3 blood samples
Test the 5th, 10,15 day respectively after feeding 2h, all individualities through jugular vein blood collection 20ml, stand, to naturally coagulating
Gu, 3000rpm is centrifuged 10 minutes, it is thus achieved that serum, and-20 degree preserve in case Biochemical Indices In Serum analysis.Biochemical Indices In Serum includes
Total protein, albumin, blood urea nitrogen, glucose, cholesterol are with automatic clinical chemistry analyzer analysis (Hitachi 7020).
2.5 statistical analysis
Data acquisition is usedMIXED program in software analyzes (SAS Institute, 2000).Result of the test is with average
Number and SEM represent.
3 interpretations of result
3.1 growth performance
Found out by table 6, use sweet potato dregs fermented feed that sheep feed intake is not had negative effect, add 2.3% on the contrary,
But difference is not notable (P < 0.05).The increase of feed intake may improve feedstuff with sweet potato dregs palatability after fermenting is relevant.
Using sweet potato dregs fermented feed Substitute For Partial rapeseed cake and Semen Maydis to improve the daily gain of sheep, test group compares matched group
Add 3.4%.The feed-weight ratio (weightening finish: search for food) of test group and matched group is not significantly different from.This explanation, when using sweet potato dregs
During fermented feed, the production performance to sheep has no adverse effect.Using so that 7% in daily ration can be accounted for of sweet potato dregs fermented feed.
The impact on sheep growth performance of the table 6 sweet potato dregs fermented feed
3.2 physiochemical indice
As shown in table 7, use sweet potato dregs fermented feed substitute in daily ration rapeseed cake and part Semen Maydis to sheep blood total protein,
The content of albumin, blood glucose and blood urea nitrogen has no significant effect (P > 0.05).Illustrate to utilize sweet potato dregs fermented feed to sheep
Nutriture and nitrogen metabolism all have no adverse effect.
Table 7 ferments the impact on sheep physiochemical indice of the sweet potato dregs feedstuff
* represent that P < 0.05, NS represents zero difference.The different lowercase alphabet of colleague's shoulder mark shows significant difference (P < 0.05).
To sum up, sweet potato dregs fermented feed replacement rapeseed cake and part Semen Maydis is used not to affect the growth performance of sheep.Sweet potato dregs
Fermented feed consumption can account for the 7% of daily ration.
The application in beef cattle feeds of the sweet potato dregs fermented feed of the embodiment present invention
1 MATERIALS METHODS
1.1 test material
By fermentation time 24h, rumen fluid inoculum concentration is 8%, and carbamide adds the method for 1% and makes fermented feed.Obtain
The true albumen of sweet potato dregs fermented feed is 29.8%, and crude fat is 4.47.
1.2 EXPERIMENTAL DESIGN
Test and set 2 process daily rations: be i.e. matched group (C), feed common daily ration (including Leymus chinensis (Trin.) Tzvel. and concentrate)；Fermented feed group
(RF), with fermented feed Substitute For Partial Semen Maydis and rapeseed cake.Experimental basis daily ration and test daily ration are shown in Table 8.
Experimental animal selects the miscellaneous beef cattles of peace that 60 dresses are healthy, carries out matched-pair design, divides by age in days, the body weight principle that is close
Become 2 groups, often group 30.Test sheep supply daily ration every day twice, freely drink water.Test is just trying the phase 30 days, raises the phase in advance 7 days.Initially
Body weight is about 106 kilograms.
Table 8 daily ration composition and chemical component table thereof
1.3 trial
Cowshed repairing, sterilization is carried out, to reduce cowshed environmental health and the parasite shadow to cattle Nutrition and Metabolism before prerun
Ring.Unified number after random packet, uses and raises by component hurdle.Raise (every morning 10 and lower 5 points) fixing special messenger to feed,
And sufficient clean drinking-water is provided in cowshed.And ensure the health and epidemic prevention conditions and environment condition that column home is good.During test
Note observation search for food, disease event.
2 measure project and method
2.1 sample analysis
Each process group feedstuff measures the conventional nutrients content such as dry, crude protein.
2.2 growth performance
Test is responsible for by special messenger, is responsible for experimental animal raising, Cheng Chong, record.
Feed intake measures weekly.6:30 weighs on an empty stomach in the morning, measures feed intake, calculates that each process group is per day adopts respectively
Appetite (ADFI), daily gain (ADG) and gain feed ratio (G/F).
The collection of 2.3 blood samples
Test the 5th, 10,15 day respectively after feeding 2h, all individualities through jugular vein blood collection 20ml, stand, to naturally coagulating
Gu, 3000rpm is centrifuged 10 minutes, it is thus achieved that serum, and-20 degree preserve in case Biochemical Indices In Serum analysis.Biochemical Indices In Serum includes
Total protein, albumin, blood urea nitrogen, glucose, cholesterol and triglyceride are with automatic clinical chemistry analyzer analysis (Hitachi 7020).
2.5 statistical analysis
Data acquisition is usedMIXED program in software analyzes (SAS Institute, 2000).Result of the test is with average
Number and SEM represent.
3 interpretations of result
3.1 growth performance
Found out by table 9, use sweet potato dregs fermented feed that beef cattle feed intake is not had negative effect, add 2.8% on the contrary,
But difference is not notable (P < 0.05).The increase of feed intake may improve feedstuff with sweet potato dregs palatability after fermenting is relevant.
Use sweet potato dregs fermented feed Substitute For Partial rapeseed cake and Semen Maydis to improve the daily gain of beef cattle, add bamboo shell group average
Daily gain height matched group 5.0%, and the weightening finish of test group is searched for food and is added 2.1% than matched group, this explanation, when using sweet potato dregs
During fermented feed, the appetite of beef cattle can be improved, and the most helpful to feed conversion rate, and this also causes increasing day by day of test group beef cattle
Weight and weightening finish search for food ratio higher than matched group.Therefore, cattle diet use sweet potato dregs fermented feed the most negative to Beef Cattle Growth
Impact.
The impact on Beef Cattle Growth performance of the table 9 sweet potato dregs fermented feed
3.2 physiochemical indice
As shown in table 10, daily ration uses sweet potato dregs fermented feed to the blood total protein of beef cattle, albumin, blood glucose and
The content of blood urea nitrogen has no significant effect (P > 0.05).The blood urea nitrogen content difference of test group and matched group beef cattle is the most notable
Illustrate daily ration to use sweet potato dregs fermented feed beef cattle nitrogen metabolism is had no adverse effect.It addition, test group and matched group beef cattle
Blood triglyceride and total cholesterol level be also not significantly different from, illustrate that the lipid metabolism of beef cattle is not affected.
Table 10 ferments the impact on beef cattle physiochemical indice of the sweet potato dregs feedstuff
* represent that P < 0.05, NS represents zero difference.The different lowercase alphabet of colleague's shoulder mark shows significant difference (P < 0.05).
To sum up, sweet potato dregs fermented feed replacement rapeseed cake and part Semen Maydis is used not to affect the growth performance of beef cattle.To beef cattle
Physiochemical indice there is no harmful effect yet.Sweet potato dregs fermented feed consumption can account for the 7% of daily ration.
Embodiment 2:
The sweet potato dregs fermented feed of a kind of ruminant, described fermented feed is inoculation cud in solid-state fermentation culture medium
Obtaining after liquid after fermentation 24h, rumen fluid inoculum concentration is 8%；Described solid-state fermentation culture medium includes in terms of mass fraction
Following component: sweet potato dregs 45%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, carbamide 1%, surplus is water.Sweet potato dregs powder in advance
It is broken into particle diameter in the range of 0.5-2mm.
Embodiment 3:
The sweet potato dregs fermented feed of a kind of ruminant, described fermented feed is inoculation cud in solid-state fermentation culture medium
Obtaining after liquid after fermentation 24h, rumen fluid inoculum concentration is 10%；Described solid-state fermentation culture medium includes in terms of mass fraction
Following component: sweet potato dregs 40%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.5%, carbamide 2%, surplus is water.Sweet potato dregs powder in advance
It is broken into particle diameter in the range of 0.5-2mm.
Embodiment 4:
The sweet potato dregs fermented feed of a kind of ruminant, described fermented feed is inoculation cud in solid-state fermentation culture medium
Obtaining after liquid after fermentation 26h, rumen fluid inoculum concentration is 8%；Described solid-state fermentation culture medium includes in terms of mass fraction
Following component: sweet potato dregs 50%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, carbamide 1%, surplus is water.Sweet potato dregs powder in advance
It is broken into particle diameter in the range of 0.5-2mm.
Embodiment 5-10:
A kind of sheep (beef cattle) feeds Diet Formula, and this formula is become by the mixed raw material described in table 11.
Table 11 (unit: kg)
Present invention sweet potato dregs fermented feed substitutes high-quality protein feed, and the offal treatment solving processing of farm products is asked
Topic, makes ruminant (cattle, sheep etc.) aquaculture cost be substantially reduced.Embodiment described above be the one of the present invention preferably
Scheme, not makees any pro forma restriction, on the premise of without departing from the technical scheme described in claim to the present invention
Also has other variant and remodeling.
Claims (6)
1. the sweet potato dregs fermented feed of a ruminant, it is characterised in that: described fermented feed is in solid-state fermentation culture medium
Upper inoculation rumen fluid after fermentation 24h is derived above, and rumen fluid inoculum concentration is 7-10%；Described solid-state fermentation culture medium includes
Following component in terms of mass fraction: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, carbamide 1% ~
3%, surplus is water.
2. the preparation method of the sweet potato dregs fermented feed of the ruminant described in a claim 1, it is characterised in that: described
Each raw material in solid-state fermentation culture medium is dissolved in suitable quantity of water the most respectively, is mixed in proportion after being sterilized separately, and supplies the water of formula ratio
After, inoculate the rumen fluid after impurity screening, in constant incubator, cultivate more than 24h for 30 DEG C.
Preparation method the most according to claim 1, it is characterised in that: described incubation time is 24-26h.
4. the application in sheep or beef cattle feed of the sweet potato dregs fermented feed described in a claim 1, it is characterised in that: institute
State sweet potato dregs fermented feed consumption and can account for the 6%-15% of sheep or cattle diet.
5. a sheep feeds Diet Formula, it is characterised in that this formula includes the component of following mass fraction:
Leymus chinensis (Trin.) Tzvel. 40-50%,
Grass 17-20%,
Semen Maydis 16-18%,
Bean cake 5-6%,
Wheat bran 3-4%,
Sal 0.5-0.8%,
Calcium hydrogen phosphate 0.5-0.8%,
Sweet potato dregs fermented feed 6-15%.
6. a beef cattle feeds Diet Formula, it is characterised in that this formula includes the component of following mass fraction:
Leymus chinensis (Trin.) Tzvel. 20-30%,
Silagecorn 18-38%,
Semen Maydis 24-26%,
Bean cake 6-8%,
Wheat bran 4-6%,
Sal 0.5-0.8%,
Calcium hydrogen phosphate 0.5-0.8%,
Sweet potato dregs fermented feed 6-15%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106721028A (en) * | 2017-01-12 | 2017-05-31 | 江苏富海生物科技有限公司 | A kind of milk cow fermented feed and preparation method thereof |
| CN113545418A (en) * | 2021-08-10 | 2021-10-26 | 华中农业大学 | Application of yellow water in ruminant animal feeding |
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| CN101965903A (en) * | 2010-08-17 | 2011-02-09 | 钱国英 | Preparation method of fermented vegetable protein |
| CN102934736A (en) * | 2012-12-05 | 2013-02-20 | 蒋常德 | Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed |
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| CN103371271A (en) * | 2012-04-30 | 2013-10-30 | 罗福仲 | Processing method for biological fermentation feed auxiliary material with sweet potato peel slag |
| CN103749967A (en) * | 2014-01-27 | 2014-04-30 | 中国农业科学院饲料研究所 | Sweet potato residue fermentation feed and preparation method thereof |
| CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
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| CN101965903A (en) * | 2010-08-17 | 2011-02-09 | 钱国英 | Preparation method of fermented vegetable protein |
| CN103371271A (en) * | 2012-04-30 | 2013-10-30 | 罗福仲 | Processing method for biological fermentation feed auxiliary material with sweet potato peel slag |
| CN102934736A (en) * | 2012-12-05 | 2013-02-20 | 蒋常德 | Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed |
| CN103271224A (en) * | 2013-06-05 | 2013-09-04 | 吉首大学 | Method for producing single-cell protein feed by means of fermentation of sweet potato residues |
| CN103749967A (en) * | 2014-01-27 | 2014-04-30 | 中国农业科学院饲料研究所 | Sweet potato residue fermentation feed and preparation method thereof |
| CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106721028A (en) * | 2017-01-12 | 2017-05-31 | 江苏富海生物科技有限公司 | A kind of milk cow fermented feed and preparation method thereof |
| CN106721028B (en) * | 2017-01-12 | 2020-05-22 | 江苏富海生物科技有限公司 | Fermented feed for dairy cows and preparation method thereof |
| CN113545418A (en) * | 2021-08-10 | 2021-10-26 | 华中农业大学 | Application of yellow water in ruminant animal feeding |
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