CN105918649A - Additive for improving rumen fermentation in ruminants and method thereof - Google Patents
Additive for improving rumen fermentation in ruminants and method thereof Download PDFInfo
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Abstract
The present invention discloses an additive for improving rumen fermentation in ruminants and a method thereof. The additive is selected from one or more of the following plant essential oils: folium eucalypti oil, eugenol, carvacrol, anethole, thymol, D-limonene and cinnamaldehyde. The provided additive is one or more plant essential oils, does not cause undesirable side effects after the animals are fed by the additive, and does not bring excreta contamination to the environment. In addition, the obtained essential oils are used as the additive for improving the animal rumen fermentation, can promote the rumen fermentation, also reduce the rumen protein degradation, reduce the ammonia nitrogen concentration, and have functions of resisting bacteria.
Description
Technical field
The present invention relates to the additive in ruminant breeding process, be specifically related to a kind of raising ruminant
The plants essential oil additive of fermentation efficiency and method thereof in cud.
Background technology
Ruminate and refer to that the food of half digestion in cud is returned the most again after after a while by feed
Chew, be a ruminant step obtaining energy process from plant cell wall.Ruminate and reduce fibre
The size of dimension granule, exposes sugar for fermentable；It addition, buffer substance (carbonate in saliva
And phosphate) neutralize the acid that fermentable produces, in order to maintain one to be conducive to fiber degradation and tumor
The environment of the neutral meta-acid of stomach growth of microorganism.Ruminant is exactly the animal ruminating phenomenon, it is common that
Some herbivores because the fiber of plant to be comparison heavy.Ruminant searches for food and typically compares hurriedly
Busy, particularly roughage, major part just swallows entrance cud without fully chewing, soak through rumen bypass and
After softening a period of time, food comes back to oral cavity through inverse vomitting, and through chewing, is again mixed into saliva also again
Swallow the process entering cud again.
Cud is that food enters first contact " darkroom " in ruminant gastrointestinal tract, is also food digestion
" main battle ground ".It is an airtight live body fermentation tank, perches micro-life of numerous species inside jar
Thing, mainly includes protozoon, antibacterial and fungus three major types.Generally, each milliliter of rumen fluid just contains
Hundred million antibacterials of 160-400,200,000 ciliate and substantial amounts of fungus.Can not look down upon these microorganisms,
They cud play the specific actor of digestive physiology function.After food arrives cud, a large amount of micro-lifes
Thing is immediately close to the surface of food, simultaneously eccrine fiber element enzyme, hemicellulase and beta-glycosidase etc.
Digestive enzyme, then the polysaccharose substance such as the cellulose in food, hemicellulose and pectin is soon by " cutting
" become monosaccharide, and " variable body " is volatile fatty acid and CO2Deng, the former can be that ruminant provides
60～the energy source of 70%.Food in cud after microorganism fully digests, the crude fibre of about 50% can
In cud digested, its fermentation end products is volatile fatty acid, volatile fatty acid pass through cud wall
Absorb and become the energy source that ruminant is main.
At present, in order to improve the fermentation efficiency of ruminant, add some chemical addition agents to plant and raise
In material, but along with deepening continuously of scientific research have also discovered many problems, people the most gradually recognize
The negative effect that these chemical addition agents are brought, so people are more likely to select natural green interpolation
Agent.Plants essential oil (essential oil) be a class be present in plant and have aromatic odor, can be with water
Steam distillation out but the general name of water-fast volatility oily mater, it be from the flower of plant, leaf,
In stem, root or fruit, refine extraction by steam distillation, squeezing and pressing method, cold-maceration or solvent extraction method
The volatile flavor taken, is one of active component important in plant extract.Therefore carrying out can
Improve ruminant tumor gastric fermentation efficiency, and the research of the Pure natural plant essential oil not having side effects shows
Obtain particularly important.
Summary of the invention
It is an object of the invention to provide one to send out for improving ruminant tumor gastric for the problems referred to above
The additive of ferment.
For achieving the above object, the present invention uses following technical method:
Present invention firstly provides a kind of additive for improving ruminant tumor gastric fermentation, above-mentioned interpolation
Thing is selected from one or more plants essential oils following: eucalyptus oil, acetaminol, carvacrol, anethole, hundred
In fragrant phenol, D-limonene or cinnamic aldehyde.
One preferred aspect of the present invention, above-mentioned additive is selected from following a kind of plants essential oil: eucalyptus oil,
Acetaminol, carvacrol, anethole, thymol, D-limonene or cinnamic aldehyde.
Further, above-mentioned additive is selected from one or more plants essential oils following: thymol, Apium graveolens
Phenol, D-limonene, anethole or eucalyptus oil.
Another preferred aspect of the present invention, above-mentioned additive is selected from following a kind of plants essential oil: Herba thymi vulgaris
Phenol, carvacrol, D-limonene, anethole or eucalyptus oil.
Further, above-mentioned additive is selected from following a kind of plants essential oil: eucalyptus oil.
Wherein, using above-mentioned additive to carry out addition when In Vitro Fermentation is cultivated is that: 50mg/L ferments training
Nutrient solution≤interpolation concentration≤400mg/L fermentation culture.
Further, can be optimized for 50mg/L fermentation culture≤add dense for the addition of above-mentioned additive
Degree≤200mg/L fermentation culture.
Another object of the present invention is to provide a kind of method for improving ruminant tumor gastric fermentation.
Said method comprises the following steps: above-mentioned additive adds feeding to the daily ration of ruminant
Ruminant.
One preferred aspect of the present invention, gives institute with the amount of every kg animal weight 7.0～9.0mg every day
State additive.
Wherein, the daily ration of above-mentioned ruminant using Semen Maydis, bean cake and Leymus chinensis (Trin.) Tzvel. as primary raw material, essence slightly than
For 6:4.
Beneficial effects of the present invention: the present invention has been drawn by screening and can improve ruminant tumor gastric fermentation
Additive, this additive is one or more plants essential oils, and they are that a class is present in plant and has
Have aromatic odor, can with vapor distillation out but the general name of water-fast volatility oily mater,
It is from the flower of plant, leaf, stem, root or fruit, by steam distillation, squeezing and pressing method, merceration
Method or solvent extraction method refine the volatile flavor of extraction, are activity one-tenth important in plant extract
/ mono-, produce undesirable side effect after the most there is not feeding animals, also will not bring Excreta
The problem polluting environment.Additionally, the present invention obtains plants essential oil as the interpolation improving animal rumens fermentation
Thing, can not only promote rumen zymosis, moreover it is possible to reduce ruminal soluble protein degraded, reduces ammonia nitrogen concentration, and
And there is antibacterial action.
Detailed description of the invention
In order to solve to promote the problem that rumen zymosis pure natural plant additive lacks at present, this test utilizes
Eucalyptus oil, litsea cubeba oil, Oleum Cinnamomi and Oleum Anisi Stellati that China's yield occupies first place in the world and at present research are relatively
Many acetaminol, D-hesperidene, anethole, cinnamic aldehyde, thymol and carvacrol totally 10 kinds of plants
Quintessence oil, as additive, studies its impact on vitro Rumen fermentation.
Below in conjunction with specific embodiment, the present invention will be described in detail, but should not be construed as the present invention
Restriction.On the premise of without departing substantially from present invention spirit and essence, modification made for the present invention or replace
Change, belong to scope of the invention:
Embodiment 1
1.1 test plant quintessence oils
Table 1 test plant quintessence oil information
1.2 culture substrate and EXPERIMENTAL DESIGN
The culture substrate of in vitro tests is with reference to the trophic level of domestic meat sheep daily ration, with Semen Maydis, bean cake and sheep
Grass, as raw material, pulverized 0.5mm sieve, by a definite proportion row preparation substrate, substrate composition and nutritional labeling
It is shown in Table 2.Test uses single factor experiment design, uses 10 kinds of plants essential oils, and respectively eucalyptus oil, mountain is grey
Seed oil, Oleum Cinnamomi, Oleum Anisi Stellati, acetaminol, D-hesperidene, anethole, cinnamic aldehyde, thymol and
Carvacrol, every kind of plants essential oil adds 0,50,100,200 and 400mg/L fermentation culture totally 5 respectively
Individual Concentraton gradient, each process sets 3 repetitions.
Table 2 gas production in vitro test culture substrate composition and nutritional labeling
1.3 donor animal feedings and managements and the collection of rumen fluid
4 body weight 67 ± 1.2kg, healthy and equipped with permanent rumen fistula Du × cold F1 generation are selected to be hybridized to
Year, sheep was as rumen fluid donor sheep.Test sheep individual pen is raised, and every day, Basic drawing quantity delivered was 1.3 times of maintenances
The raise competence needed, the thick ratio of daily ration essence for 6:4, feeds twice in 8:00 and 18:00, interpolation at noon every day
Radix Glycyrrhizae once, is freely drunk water.
Raise front 2h morning and gathered the rumen content of 4 Fistulated sheep by rumen fistula, after mixing, be loaded on insulation
Laboratory taken back rapidly by bottle, (filters by 2 layers of filtered through gauze and is passed through CO simultaneously2), whole operate in 39 DEG C
Carrying out in water-bath, operation should complete within the shortest time.
1.4 vitro Rumen fermentation culture
Test uses the In Vitro gas production method of Menke etc., gathers 4 Fistulated sheep installing permanent rumen fistula
Rumen fluid, is mixed and made into artificial rumen culture fluid with buffer with the ratio of 1:2.Weigh 200mg (DM
Basis) substrate is in 100mL culture tube (syringe), and every kind of substrate is weighed in 3 culture tubes respectively,
Before adding artificial rumen culture fluid, inject 0.5mL variable concentrations gradient from each syringe front end
Plants essential oil mixture.Conventionally preparation artificial rumen culture fluid, with automatic liquid adding device to each
Syringe is separately added into 30mL culture fluid so that the plants essential oil concentration of different disposal group respectively reaches
0,50,100,200 and 400mg/L fermentation culture.Glass Culture Tubes liquid feeding end is arranged straight up
Although interior gas, clamp front end silica gel tube with PC, and record corresponding initial scale value (mL), with
Time do 3 blank (only culture fluid and do not have substrate).Culture tube is put into rapidly warmed-up (39 DEG C)
Artificial rumen incubator in cultivate 24h, record culture tube is in the scale value of different time points, and in 24h
Terminate cultivating, measure the every fermentation parameter of fermentation liquid.
10 kinds of plants essential oils the results are shown in Table 3 to the impact of In Vitro Fermentation pH value:
The impact on vitro Rumen fermentation pH of the table 3 different plants essential oil
Colleague's data are takeed on mark different lower case a, b and are represented significant difference (P < 0.05), identical or the most notable without letter shoulder mark expression difference
(P>0.05).Linear and the conic section effect that L, Q are respectively between each process group.
As can be seen from Table 3, add above-mentioned 10 kinds of quintessence oils, except litsea cubeba oil, Oleum Anisi Stellati and thymol,
Other plant quintessence oil the most non-appreciable impact ruminal pH value (P > 0.05), and 50mg/L fermentation culture≤
Add concentration≤200mg/L interpolation in the range of, compared with matched group, add variable concentrations Oleum Anisi Stellati and
Thymol also has no significant effect (P > 0.05) to ruminal pH value.As can be seen here, the adding of plants essential oil
Add, ruminal pH value is not produced and significantly affects.
10 kinds of different quintessence oils are shown in Table 4 to the impact of vitro Rumen fermentation VFA content:
The impact on vitro Rumen fermentation VFA content of the table 4 different plants essential oil
Colleague's data are takeed on mark different lower case a, b and are represented significant difference (P < 0.05), identical or the most notable without letter shoulder mark expression difference
(P>0.05).Linear and the conic section effect that L, Q are respectively between each process group.
As can be seen from Table 4, add eucalyptus oil, acetaminol, D-limonene, anethole, cinnamic aldehyde,
Thymol and carvacrol, overall beneficial is in improving cud general volatile fatty acid (VFA) content.Its
In, add thymol and carvacrol appreciable impact (P < 0.01) In Vitro Fermentation liquid total VFA content, and
The content increasing total VFA with thymol and carvacrol concentration is conic section ascendant trend (Q；
P<0.05).And other several plant quintessence oil eucalyptus oil, acetaminol, D-limonene, anethole, meat
The interpolation of cinnamic aldehyde, the impact all not up to significance level (P > 0.05) on total VFA, but and matched group
Comparing, the content of total VFA the most all increases.Add D-limonene and anethole appreciable impact
The proportion of acetic acid of (P < 0.01) vitro Rumen fermentation liquid, compared with matched group, adds 50mg/L D-Fructus Citri Limoniae
Alkene dramatically increases (P < 0.01) proportion of acetic acid, adds 200mg/L D-limonene and significantly reduces (P=0.04)
Proportion of acetic acid, and linearly decline (L with D-limonene concentration increase；P < 0.01) trend；Add 50mg/L
Anethole dramatically increases (P=0.02) proportion of acetic acid, and linearly declines (L with anethole concentration increase；
P < 0.01) trend.And other plant quintessence oil is at 50mg/L fermentation culture≤interpolation concentration≤200mg/L
Interpolation in the range of, proportion of acetic acid is all had no significant effect (P > 0.05).
Add eucalyptus oil, D-limonene, anethole, thymol and carvacrol appreciable impact (P < 0.01)
The propionic acid content of vitro Rumen fermentation liquid.Wherein, with eucalyptus oil add concentration increase, propanoic acid ratio in
Conic section rises (Q；P < 0.01) trend；Compared with matched group, add 100mg/L and 200mg/L
D-limonene dramatically increases (P < 0.01) propanoic acid ratio, and presents secondary with the increase of D-limonene concentration
Curve rises (Q；P < 0.01) trend；Add 50mg/L anethole and significantly reduce (P < 0.01) propanoic acid
Ratio, and present conic section rising (Q with the increase of anethole concentration；P < 0.01) trend；Add
200mg/L thymol significantly reduces (P < 0.01) propanoic acid ratio, and with the increase of thymol concentration
Linearly decline (L；P=0.02) trend；Add the increase of concentration with carvacrol, propanoic acid ratio is secondary
Curvilinear motion (L；P < 0.01) trend.
Add eucalyptus oil, D-limonene, anethole and carvacrol appreciable impact (P < 0.05) In Vitro Fermentation
Liquid acetic acid and propanoic acid ratio.Wherein, acetic acid and propanoic acid ratio are conic section with the increase of eucalyptus oil concentration
Decline (Q；P < 0.01) trend；Add 100mg/L and 200mg/L D-limonene and significantly reduce (P < 0.01)
Acetic acid and propanoic acid ratio, and be that conic section declines (Q with the increase of D-limonene concentration；P < 0.01) become
Gesture；Add 50mg/L anethole and significantly improve (P < 0.01) acetic acid and propanoic acid ratio, add 200mg/L
Anethole from numerically reducing (P=0.05) acetic acid and propanoic acid ratio, and with anethole concentration increase in
Conic section change (Q；P < 0.01) trend；With the increase of carvacrol concentration, acetic acid and propanoic acid ratio in
Linear rise (L；P < 0.01) trend.
Vitro Rumen is fermented NH by various plants essential oils3The impact of-N the results are shown in Table 5:
Vitro Rumen is fermented NH by the different plants essential oil of table 53The impact of-N concentration
Colleague's data are takeed on mark different lower case a, b and are represented significant difference (P < 0.05), identical or the most notable without letter shoulder mark expression difference
(P>0.05).Linear and the conic section effect that L, Q are respectively between each process group.
As can be seen from Table 5, in the interpolation scope of 50mg/L fermentation culture≤interpolation concentration≤200mg/L
In, compared with matched group, vitro Rumen is fermented NH by the various plants essential oils adding variable concentrations3-N's
Produce and all have no significant effect (P > 0.05).
Based on the above results it can be seen that eucalyptus oil, acetaminol, D-limonene, anethole, Cortex Cinnamomi
Aldehyde, thymol or carvacrol are respectively provided with the effect promoting vitro Rumen fermentation.
Wherein, thymol, carvacrol, D-limonene, anethole or eucalyptus oil promote that vitro Rumen is sent out
The effect of ferment is obvious.
Embodiment 2
In the present embodiment the eucalyptus oil screened in above-mentioned test and Oleum Anisi Stellati are carried out animal further
Test.
2.1 experimental animals and feeding and management
6 the healthy Du Han F1 generation adult Fistulated sheep of hybridization selecting body weight to be 64.45 ± 8.56kg, at random
It is divided into 3 groups, often 2 repetitions of group.The most single cage of test sheep is raised, and feeds every day 2 times, respectively at 8:00 morning
With 6:00 feeding experiment daily ration in evening, free choice feeding, record the feeding capacity of every and surplus doses, keep every
Surplus doses account for about the 5% of feeding capacity.During test, all sheep only freely drink water.
Plants essential oil scale of feeding is the 0.5g/ previous day.
2.2 test daily ration and EXPERIMENTAL DESIGN
Test is divided into 3 process, and 2 sheep of each process, respectively (basal diet does not adds matched group
Add quintessence oil), Folium eucalypti globueli (Eucalyptus globulus Labill.) line of oils (basal diet+0.5g eucalyptus oil/previous day), Fructus Foeniculi line of oils (basal diet
+ 0.5g Oleum Anisi Stellati/previous day).Using 3 × 3 Latin square design, 3 phases of carrying out test, each issue 22d, its
In raise phase 14d, sampling date 7d in advance.The digest supersession test wherein carrying out 4d in the 15-18 days, in digestion
Before metabolic test, allow test sheep shift to an earlier date 2d and enter digestion and metabolism cage adaptation environment, within the 22nd day, take rumen fluid.
Test daily ration: test daily ration, according to China's meat sheep feeding standard (2004) preparation, makes granule and raises
Material, daily ration essence is slightly than 6:4.
2.3 Rumen Fermentations measure
In d22 last day of each issue test, 0 after raising morning, 1,3,6 and 9h from the cud of every sheep
Fistula gathers about 30mL rumen fluid, through 2 layers of filtered through gauze, first measures pH, and keep sample mensuration subsequently
VFA、NH3-N, and carry out protozoon counting.
(1) pH measures: measure rumen fluid ph with portable electronic pH meter rapidly after having gathered rumen fluid.
(2) VFA keeps sample: 5mL filters rumen fluid and adds 25% (wt/vol) phosphorus partially of the pre-cold preservation of 1mL
Acid (HPO3) solution ,-20 DEG C of freezen protective.Use gas chromatography determination VFA concentration.
(3) NH3-N keeps sample: 5mL filters rumen fluid and adds the 1%H of the pre-cold preservation of 1mL2SO4Solution,
-20 DEG C of freezen protective.Use NH in phenol-sodium hypochlorite colorimetric method for determining rumen fluid3The concentration of-N.
(4) protozoon counting: 1mL filtrate adds 5mL C.I. 42590 formalin saline solution (methyl
Green-formalin-saline) in, room temperature keeps in Dark Place.Counted by blood counting chamber under the microscope.
2.4 digest supersession test
In advance the phase of raising terminate after start digest supersession test, 2 days before digest supersession test starts
(d13-14) proceeding to test sheep adapt in metabolic cage, (d15-18) that carry out subsequently continuous 4 days receives
Excrement and receipts urine.
During digest supersession test, every day gathers feedstuff sample, and accurately record feed intake and surplus doses.Adopt
Collect excrement, urine by full fecaluria method of receiving, weigh and record every sheep Fecal wastes every day, during sampling, excrement sample is mixed
Even, take the Subsampling not polluted by Pilus Caprae seu Ovis and dust from different loci, accurately weigh the 10% of total excrement amount
Loading freezen protective (-20 DEG C) in valve bag, the excrement sample freezen protective respectively of every sheep every day, in case surveying
Determine the related data such as various nutritional labelings in digestibility, excrement.During mensuration, sample is put in 65 DEG C of baking ovens
Put 48h, weigh after moisture regain 24h, measure first moisture in excrement, grind and cross 0.5mm sieve subsequently standby
With；Every day entirely receives urine and records urine volume, with filling 100mL10%H2SO4Plastic Drum collect urine,
By 10% proportional sampling, the urine sample freezen protective respectively of every sheep every day.
The dry in feedstuff and excrement is measured according to " forage analysis and determination of feeds quality technology " method
(DM), Organic substance (OM), crude protein (CP), neutral detergent fiber (NDF), acidity are washed
Wash fiber (ADF), calcium (Ca), phosphorus (P).
2 kinds of plants essential oils the results are shown in Table 6 to the impact of meat sheep rumen zymosis:
Table 6 eucalyptus oil and the Oleum Anisi Stellati impact on meat sheep rumen zymosis
Significant difference (P < 0.05) is represented according to takeing on mark different lower case a, b with row, identical or the most notable without letter shoulder mark expression difference
(P>0.05)。
As can be seen from Table 6,0.5g/ eucalyptus oil on the previous day and Oleum Anisi Stellati are added to meat sheep rumen fluid ph without shadow
Ring (P > 0.05)；Add 0.5g/ eucalyptus oil on the previous day and Oleum Anisi Stellati to meat sheep cud general volatile fatty acid
(VFA) concentration does not makes significant difference (P > 0.05)；Add 0.5g/ eucalyptus oil on the previous day and Oleum Anisi Stellati to meat
Sheep cud various VFA ratio does not all make significant difference (P > 0.05)；Add 0.5g/ eucalyptus oil on the previous day and fennel
Oleum sesami is to meat sheep cud NH3-N concentration does not makes significant difference (P > 0.05) yet.
2 kinds of plants essential oils the results are shown in Table 7 to the impact of meat sheep rumen metabolism quantity:
Table 7 eucalyptus oil and Oleum Anisi Stellati are on the impact of protozoon total amount in meat sheep cud
Significant difference (P < 0.05) is represented according to takeing on mark different lower case a, b with row, identical or the most notable without letter shoulder mark expression difference
(P>0.05)。
As can be seen from Table 7, add 0.5g/ eucalyptus oil on the previous day and Oleum Anisi Stellati to caterpillar in meat sheep cud,
Bistrichiasis worm, capillus worm, etc. caterpillar and protozoon sum all do not make significant difference (P > 0.05).
2 kinds of plants essential oils the results are shown in Table 8 to the impact of meat sheep feed intake and nutritive digestibility:
Table 8 eucalyptus oil and Oleum Anisi Stellati are on meat sheep nutrient substance feed intake and the impact of apparent digestibility
Colleague's data are takeed on mark different lower case a, b and are represented significant difference (P<0.05), identical or notable (P>0.05) without letter shoulder mark expression difference
As can be seen from Table 8, add eucalyptus oil and meat sheep feed intake is not made significant difference by Oleum Anisi Stellati
(P=0.20).Compared with matched group, add 0.5g/ eucalyptus oil on the previous day to meat sheep dry (DM),
Organic substance (OM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF)
Apparent digestibility all do not make significant difference (P > 0.05), but the interpolation of eucalyptus oil, to meat sheep DM, OM,
The apparent digestibility of CP and ADF numerically increases, although being not significantly different from statistically,
But the most meaningful on producing, the interpolation of eucalyptus oil is described, to improving meat sheep nutritive digestibility
There is certain facilitation.Add 0.5g/ Oleum Anisi Stellati on the previous day, to meat sheep DM, OM, CP, NDF
All do not make significant difference with the apparent digestibility of ADF (P > 0.05).
In sum, meat sheep rumen zymosis is had some improvement by eucalyptus oil, and does not produce other
The side effect of adverse effect, can be as a kind of preferred plant quintessence oil added in daily ration.
Claims (10)
1. the additive being used for improving ruminant tumor gastric fermentation, it is characterised in that selected from such as next
Plant or various plants quintessence oil: eucalyptus oil, acetaminol, carvacrol, anethole, thymol, D-lemon
Lemon alkene or cinnamic aldehyde.
Additive the most according to claim 1, it is characterised in that selected from following a kind of plants essential oil:
Eucalyptus oil, acetaminol, carvacrol, anethole, thymol, D-limonene or cinnamic aldehyde.
Additive the most according to claim 1 and 2, it is characterised in that selected from following a kind of or many
Kind plants essential oil: thymol, carvacrol, D-limonene, anethole or eucalyptus oil.
Additive the most according to claim 3, it is characterised in that selected from following a kind of plants essential oil:
Thymol, carvacrol, D-limonene, anethole or eucalyptus oil.
Additive the most according to claim 4, it is characterised in that selected from following a kind of plants essential oil:
Eucalyptus oil.
Additive the most according to claim 1, it is characterised in that use above-mentioned additive to carry out body
Addition during outer fermentation culture is: 50mg/L fermentation culture≤interpolation concentration≤400mg/L ferments training
Nutrient solution.
Additive the most according to claim 6, it is characterised in that the addition of additive can optimize
For: 50mg/L fermentation culture≤interpolation concentration≤200mg/L fermentation culture.
8., for the method improving ruminant tumor gastric fermentation, comprise the following steps: by claim
Additive described in 1 adds to feeding ruminants in the daily ration of ruminant.
Method the most according to claim 8, it is characterised in that every day is with every kg animal weight
The amount of 7.0～9.0mg gives described additive.
Method the most according to claim 8, it is characterised in that the daily ration of described ruminant with
Semen Maydis, bean cake and Leymus chinensis (Trin.) Tzvel. are as primary raw material, and essence slightly ratio is for 6:4.
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| CN107006694A (en) * | 2017-03-20 | 2017-08-04 | 刘春海 | A kind of plant essential oil composition, preparation method and applications |
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| CN110692821A (en) * | 2019-10-21 | 2020-01-17 | 安徽农业大学 | Feed additive for improving rumen fermentation efficiency of cattle |
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| CN107006694A (en) * | 2017-03-20 | 2017-08-04 | 刘春海 | A kind of plant essential oil composition, preparation method and applications |
| CN107319141A (en) * | 2017-07-01 | 2017-11-07 | 山东阳春羊奶乳业有限公司 | A kind of kidlet feed additive and preparation method thereof |
| CN110012975A (en) * | 2019-05-11 | 2019-07-16 | 辽宁九州生物科技有限公司 | A kind of preparation method of beef cattle concentration fermentation material |
| CN110692821A (en) * | 2019-10-21 | 2020-01-17 | 安徽农业大学 | Feed additive for improving rumen fermentation efficiency of cattle |
| CN110771742A (en) * | 2019-10-21 | 2020-02-11 | 安徽农业大学 | Feed additive for reducing methane emission of rumen of cattle and preparation method thereof |
| CN110771742B (en) * | 2019-10-21 | 2022-12-20 | 安徽农业大学 | Feed additive for reducing methane emission of rumen of cattle and preparation method thereof |
| CN110692821B (en) * | 2019-10-21 | 2022-12-20 | 安徽农业大学 | Feed additive for improving rumen fermentation efficiency of cattle |
| CN111838427A (en) * | 2020-08-11 | 2020-10-30 | 南京农业大学 | Phytosterol formula for promoting rumen fermentation and determination method thereof |
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