CN106173377B - A kind of sweet potato residue fermented feed of ruminant and its application - Google Patents
A kind of sweet potato residue fermented feed of ruminant and its application Download PDFInfo
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Abstract
The present invention relates to a kind of feed, in particular to the sweet potato residue fermented feed of a kind of ruminant and its application.A kind of sweet potato residue fermented feed of ruminant, the fermented feed are that inoculation rumen fluid post-fermentation is derived above for 24 hours in solid-state fermentation culture medium, and rumen fluid inoculum concentration is 7-10%；Include following component based on mass fraction in the solid-state fermentation culture medium: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea 1% ~ 3%, surplus are water.Using rumen fluid culture technique, high protein feed both can provide, also solve the problem that problem of environmental pollution.
Description
Technical field
The present invention relates to a kind of feed, in particular to the sweet potato residue fermented feed of a kind of ruminant and its application.
Background technique
With popularizing for livestock technology, aquaculture industry of China is quickly grown.However, China per capita meat, egg, milk intake with
Developed country still has the consumption figure of the high-grade livestock products such as biggish gap, especially beef and mutton well below developed country.With hair
It is compared up to country, aquaculture industry of China Rearing facility, environmental condition are poor at present, and culture diseases are relatively more, and maximum
Problem is exactly feed resource shortage, and aquaculture cost height is caused to look forward to, and household income is relatively low.At the same time, there is also tight for China's grain
The deficiency of weight, the energy feed and protein feed of annual import increase year by year.Therefore, pass through the edible cereal of the feeding animals mankind
It is not scientific come the road that increases herding yield.How preferably developing ecology grain-saving type ruminant cultivation is that China's animal husbandry can
The only way which must be passed of sustainable development.
For sweet potato as one of China cereal crops, cultivated area accounts for about global 70%, annual output at 11.7 tons or more,
The 80% of whole world total output is accounted for, cultivated area and yield occupy the first in the world.Sweet potato dregs are sweet potato production starch and bean vermicelli
By-product in the process, fresh sweet potato slag have water content big, are not easy to store, are easy the features such as smelly of rotting, most factory
Family abandons it as waste material, not only causes the waste of resource, also serious pollution environment.Present potato slag is mainly passed through using this
Following approach: (1), fresh sweet potato slag be sealed by fermentation, be used as fresh feed.(2), dry, crush, make mixed feed, and with small casual household pig feeding
Based on.(3), the rare edible mushroom of breeding such as mushroom and oyster mushroom etc. are used to.(4), wine brewing etc..(5), the above-mentioned several approach such as fuel are made.It is sweet
The characteristic of the high starch of potato slag high microsteping causes its Direct-fed animal in aquaculture to hinder growth of animal.Except contain a small amount of starch
It outside, is largely cellulose and hemicellulose, protein content is very low, and Direct-fed animal digestion is very poor.In addition, sweet potato dregs
Containing anti-nutritional factors such as cellulose, trypsin ihhibitors, influences animal intestinal tract and nutriment is absorbed and utilized.How
Developing and utilizing sweet potato dregs resource is current some main research hotspots of scholar.
Fermented feed refers under manual control condition, and microorganism is by itself metabolic activity, by vegetalitas, animality
With in mineral substance anti-nutritional factors decompose or conversion, generate more can by livestock search for food digestion, absorb nutrient it is higher and
The feedstuff of nonhazardous effect.Sweet potato dregs nutritive value can be improved by microbial fermentation, by low quality albumen in sweet potato dregs
It is converted into high quality mycoprotein, improves albumen quality；Microorganism grows in sweet potato dregs fermentation substrate simultaneously, can degrade anti-
Trophic factors secretes benefit materials, helps to improve animal to the digestive utilization ratio of feed.
Using rumen fluid culture technique, high protein feed both can provide, also solve the problem that problem of environmental pollution.
Summary of the invention
The present invention provides a kind of sweet potato residue fermented feed of ruminant, which both may be used
High protein feed is provided, also solves the problem that problem of environmental pollution.
The technical solution adopted by the present invention to solve the technical problems is:
A kind of sweet potato residue fermented feed of ruminant, the fermented feed is that cud is inoculated in solid-state fermentation culture medium
Liquid post-fermentation is derived above for 24 hours, and rumen fluid inoculum concentration is 7-10%；It include with mass fraction in the solid-state fermentation culture medium
The following component of meter: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea 1%~3%,
Surplus is water.
A kind of preparation method of the sweet potato residue fermented feed of the ruminant, in the solid-state fermentation culture medium
Each raw material is first dissolved in suitable quantity of water respectively, mixes in proportion after being sterilized separately, after the water for supplying formula ratio, after being inoculated with impurity screening
Rumen fluid, in constant incubator 30 DEG C culture for 24 hours more than.The fermentation process used is aerobic solids fermentation, does not need to detest
The equipment and strict control condition of aerobe fermentation, it is simple and easy.Preferably, the incubation time is 24-26h.
Application of the sweet potato residue fermented feed in sheep or beef cattle feeding described in a kind of, it is characterised in that: the sweet potato
Residue fermented feed dosage can account for the 6%-15% of sheep or cattle diet.
A kind of sheep feeding Diet Formula, which includes the component of following mass fraction:
Sheep's hay 40-50%,
Green grass 17-20%,
Corn 16-18%,
Dregs of beans 5-6%,
Wheat bran 3-4%,
Salt 0.5-0.8%,
Calcium monohydrogen phosphate 0.5-0.8%,
Sweet potato residue fermented feed 6-15%.
A kind of beef cattle feeding Diet Formula, which includes the component of following mass fraction:
Sheep's hay 20-30%,
Silage corn 18-38%,
Corn 24-26%,
Dregs of beans 6-8%,
Wheat bran 4-6%,
Salt 0.5-0.8%,
Calcium monohydrogen phosphate 0.5-0.8%,
Sweet potato residue fermented feed 6-15%.
The cud of ruminant is a huge anaerobic fermentation tank, in rumen fluid containing there are many can digestibility and utilization starch and
Bacterium, fungi, protozoon of cellulose etc..Rumen fluid is taken out out of ruminant tumor gastric, is taken by installing rumen fistula to ox
Rumen fluid.The present invention substitutes high-quality protein feed with sweet potato residue fermented feed, and cost substantially reduces.
The beneficial effects of the present invention are: China's sweet potato cultivated area has 9,300,000 hectares, total output is accounted at 4.5 hundred million tons or so
The 80% of Gross World Product, therefore sweet potato dregs yield is huge.Because using rumen fluid as culture, sweet potato dregs hair produced
Ferment feed, closer to the lumen fermentation product of ox body itself, can be utilized compared with other fermented feeds by ox body good absorption,
True protein content reaches 30%.And some other fermentation roughage protein content is mostly in 10%-20%.Fistula can be installed to ox
Pipe takes rumen fluid, also can use the rumen fluid in slaughterhouse, avoids the waste of precious resources.In addition this technology directly uses solid
Fermentation process, unlike EM fermented feed etc. needs anaerobic fermentation and relevant device, and fermentation time also compares EM fermentation
Want short in 5-6 days of feed.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be further explained in detail.It should be appreciated that this hair
Bright implementation is not limited by the following examples, and the accommodation in any form made to the present invention and/or changed will all be fallen
Enter the scope of the present invention.
In the present invention, if not refering in particular to, all parts, percentage are unit of weight, used equipment and raw material etc.
It is commercially available or commonly used in the art.Method in following embodiments is unless otherwise instructed the normal of this field
Rule method.
Sweet potato dregs: certain sweet potato processing factory is come from.Rumen fluid is taken out out of ruminant tumor gastric, by installing cud to ox
Fistula takes rumen fluid.
Embodiment 1:
Solid-state fermentation culture medium: sweet potato dregs 40%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, urea 1%~6% are remaining
Amount is water.Sweet potato dregs are ground into partial size within the scope of 0.5-2mm in advance.
With fermentation time, inoculum concentration, urea additive amount for three influence factors, three levels are arranged 9 by orthogonal test
Processing group, 3 repetitions of each processing group.
In solid-state fermentation culture medium, sweet potato dregs ratio accounts for 40%.Sweet potato dregs 60% can not carry out high pressure sterilization after being watered, because
It is 121 DEG C, after 30min, sweet potato paste is in puffy, can not pour into plate.Therefore sweet potato dregs and additive (urea, magnesium sulfate, phosphoric acid
Potassium dihydrogen) aqueous solution is sterilized separately, after on superclean bench, mix in proportion, be inoculated with by 4 layers of yarn in culture dish
Rumen fluid after cloth impurity screening.In in constant incubator, 30 DEG C of culture 12h, for 24 hours, 36h.
The direct inoculation fermentation parameter of 1 cud of table
Concrete operations are: referring to table 1, solid-state fermentation culture medium 5g or so being fitted into 9cm culture dish, 121 DEG C of sterilizings
After 20min, rumen fluid is accessed by 4%, 8%, 12% (v/w) of fermented and cultured base weight in every box, box mouth covers two layers after inoculation
Sterilize wet gauze, 30 DEG C of cultures 12,24,36h, measures after 65 DEG C of drying, crushing for analysis.
Testing index
A, true albumen: test is using sweet potato dregs protein content after the measurement fermentation of Coomassie brilliant blue G250 method.Specific steps are such as
Under:
1) sample about 1g or so is weighed in 50ml centrifuge tube, 15ml0.9% physiological saline is added, and extraction is stood after oscillation
1h；
2) it is centrifuged after the completion of extraction, 2000min-1, 5min pipettes supernatant and sets 15ml centrifuge tube；
3) plus proper amount of active carbon is in the 15ml centrifuge tube for filling leaching liquor, stands 30min or more；
4)3000min-1, it is centrifuged 5min；
5) it takes supernatant 0.2ml to enter test tube to be measured, 6ml Coomassie brilliant blue stain is added, stand 5min or more, within 1h,
Visible spectrophotometer wavelength 595nm measures OD value；
6) true protein content is calculated.
B, crude fat: utilizing Soxhlet fat extractor, and the fat and liposoluble substance in dissolved samples are in ether, difference
Calculate to obtain crude fat content.
Interpretation of result
In former sweet potato dregs sample, protein content is only 3.5%, crude fat 2.3%, by strain in rumen fluid to sweet potato dregs
The fermentation of culture medium, it can be seen that the content of albumen is significantly increased, though because make slightly in culture medium added with urea
The measurement of the content of albumen has an interference, but can clearly measure containing for true albumen in sample by Coomassie brilliant blue G250 method
Amount.It is significantly improved from Table 2, it can be seen that the content of albumen has, can reach 29.63% and 29.16%, significantly
Higher than the protein content of other groups.And the content of crude fat, there has also been increasing significantly, highest has 5.32%, is higher than it significantly
His partially processing group illustrates that comparison under the processing is suitble to the generation of fat.
As shown in table 3, A is fermentation time.Under normal circumstances, in earlier fermentation substrate protein content extend at any time and
Increase, to sufficiently fermentation saturation value after a certain period of time, is reached, protein content reduction can be presented because of thallus self-dissolving later.Therefore,
Grasping reasonable fermentation time is an important factor for influencing attenuation degree.B is rumen fluid inoculum concentration.In general, early period is inoculated with
More rumen fluids give more bacterial contents first, secondly, rumen fluid also provides a better suited PH for the transition of strain
And material base.C is urea additive amount.Urea is as the source N adding ingredient, is that microorganism is numerous during bacterial multiplication early period
It grows and provides material base.
The Nutrious fermented composition transfer index of 2 rumen fluid sweet potato dregs of table
3 rumen fluid of table is directly inoculated with sweet potato dregs fermentation true protein content quadrature analysis
Remarks: factor A, B, C respectively refer to fermentation time, rumen fluid inoculum concentration and urea additive amount.
As shown in table 3, group is combined into fermentation for 24 hours, rumen fluid 12%, urea addition 1% or fermentation 36h, rumen fluid 8%,
Product true protein content is higher after fermentation when urea addition 1%, respectively reaches 29.63% and 29.16%.
Statistical analysis it is found that according to very poor R size, the factor primary and secondary sequence for influencing tunning true protein content is urine
Plain additive amount > fermentation time > inoculum concentration, according to the size of K value, the proportion that three factors are most suitable for fermentation sweet potato dregs be should be
For 24 hours, inoculum concentration 8%, urea additive amount is 1% by A2B2C1, i.e. fermentation time.
To in the quadrature analysis table 4 of tunning crude fat content, it can be seen that by very poor size, the master of influence factor
Secondary sequence should be urea additive amount > inoculum concentration > fermentation time, and the most proper combination should be A2B2C1, i.e., fermentation time for 24 hours, rumen fluid
Inoculum concentration is 8%, urea addition 1%.
4 rumen fluid of table is directly inoculated with sweet potato dregs fermentation crude fat content quadrature analysis
Remarks: factor A, B, C respectively refer to fermentation time, rumen fluid inoculum concentration and urea additive amount.
To sum up, when using mixing rumen microorganism in rumen fluid as strain fermentation sweet potato dregs, in general, with true albumen
If paying the utmost attention to crude fat index, for 24 hours for fermentation time, rumen fluid inoculum concentration is 8% to the most proper combination, urea addition 1%.
And rumen fluid is comparatively safe for feeding ruminants derived from ruminant.
Application of the embodiment sweet potato residue fermented feed of the invention in sheep feeds
1 MATERIALS METHODS
1.1 test material
For 24 hours by fermentation time, rumen fluid inoculum concentration is 8%, and the method for urea addition 1% makes fermented feed.It obtains
The true albumen of sweet potato residue fermented feed is 29.8%, crude fat 4.47.
1.2 experimental design
Test sets 2 processing daily rations: i.e. control group (C) feeds common daily ration (including sheep's hay and fine fodder)；Fermented feed group
(RF), part corn and rapeseed meal are substituted with fermented feed.Experimental basis daily ration and test daily ration are shown in Table 5.
Experimental animal selects the sheep of 60 dress health, carries out matched-pair design, is divided into 2 by age in days, the weight approximately principle that connects
Group, every group 30.Test sheep supply daily ration twice daily, free water.The test positive examination phase 30 days, pre-feeding period 7 days.Original body mass
It is 20 kilograms or so.
5 daily ration of table composition and its chemical component table
1.3 trial
Sheep hurdle repairing, disinfection are carried out before prerun, inside and outside expelling parasite is carried out to all sheep, to reduce sheep hurdle environmental sanitation and post
The infested influence to sheep Nutrition and Metabolism.Unified number after random grouping, is fed in different sheds using by group.(9 points of every morning of raising
With lower 4 points) special messenger's feeding is fixed, and sufficient clean drinking-water is provided in sheep hurdle.And guarantee the good health and epidemic prevention item of column home
Part and environmental condition.Observation feeding, disease event are paid attention to during test.
2 measurement items and method
2.1 sampling analysis
The conventional nutrients contents such as each processing group feed measurement dry matter, crude protein.
2.2 growth performance
Test is responsible for by special messenger, is responsible for experimental animal raising, Cheng Chong, record.
Feed intake measures weekly.6:30 weighs on an empty stomach in the morning, measures feed intake, calculates separately that each processing group is per day to adopt
Appetite (ADFI), daily gain (ADG) and gain feed ratio (G/F).
The acquisition of 2.3 blood samples
The 5th, 10,15 day is tested respectively after feeding 2h, all individuals are stood through jugular vein blood collection 20ml, until naturally solidifying
Gu 3000rpm is centrifuged 10 minutes, serum is obtained, -20 degree save in case Biochemical Indices In Serum is analyzed.Biochemical Indices In Serum includes
Total protein, albumin, urea nitrogen, glucose, cholesterol analyze (Hitachi 7020) with automatic clinical chemistry analyzer.
2.5 statistical analysis
Data useMIXED program analysis (SAS Institute, 2000) in software.Test result is used flat
Mean and SEM are indicated.
3 interpretations of result
3.1 growth performance
As can be seen from Table 6, not having negative effect to sheep feed intake using sweet potato residue fermented feed, 2.3% is increased instead,
But difference is not significant (P < 0.05).Increasing for feed intake may be related with the palatability of feed is improved after sweet potato dregs fermentation.
Part rapeseed meal is substituted using sweet potato residue fermented feed and corn improves the daily gain of sheep, and test group compares control group
Increase 3.4%.The feed-weight ratio (weight gain: feeding) of test group and control group is not significantly different.This explanation, when use sweet potato dregs
The production performance of sheep is had no adverse effect when fermented feed.The use of sweet potato residue fermented feed with can account in daily ration 7%.
Influence of the 6 sweet potato residue fermented feed of table to sheep growth performance
3.2 physiochemical indice
As shown in table 7, using rapeseed meal and part corn in sweet potato residue fermented feed substitution daily ration to sheep blood total protein,
The content of albumin, blood glucose and urea nitrogen has no significant effect (P > 0.05).Illustrate using sweet potato residue fermented feed to sheep
Nutrition condition and nitrogen metabolism all have no adverse effect.
Influence of the fermentation sweet potato dreg fodder of table 7 to sheep physiochemical indice
* indicate that P < 0.05, NS indicate indifference.Shoulder mark difference lowercase letter indication difference of going together is significant (P < 0.05).
To sum up, substituting rapeseed meal and part corn using sweet potato residue fermented feed does not influence the growth performance of sheep.Sweet potato dregs
Fermented feed dosage can account for the 7% of daily ration.
Application of the embodiment sweet potato residue fermented feed of the invention in beef cattle feeds
1 MATERIALS METHODS
1.1 test material
For 24 hours by fermentation time, rumen fluid inoculum concentration is 8%, and the method for urea addition 1% makes fermented feed.It obtains
The true albumen of sweet potato residue fermented feed is 29.8%, crude fat 4.47.
1.2 experimental design
Test sets 2 processing daily rations: i.e. control group (C) feeds common daily ration (including sheep's hay and fine fodder)；Fermented feed group
(RF), part corn and rapeseed meal are substituted with fermented feed.Experimental basis daily ration and test daily ration are shown in Table 8.
Experimental animal selects the miscellaneous beef cattles of peace of 60 dress health, carries out matched-pair design, connects approximately principle point by age in days, weight
At 2 groups, every group 30.Test sheep supply daily ration twice daily, free water.The test positive examination phase 30 days, pre-feeding period 7 days.Initially
Weight is 106 kilograms or so.
8 daily ration of table composition and its chemical component table
1.3 trial
Cowshed repairing, disinfection are carried out before prerun, to reduce cowshed environmental sanitation and helminth to the shadow of ox Nutrition and Metabolism
It rings.Unified number after random grouping, is fed in different sheds using by group.The fixed special messenger's feeding of raising (10 points of every morning and 5 points lower),
And sufficient clean drinking-water is provided in cowshed.And guarantee the good health and epidemic prevention conditions and environment condition of column home.During test
Pay attention to observation feeding, disease event.
2 measurement items and method
2.1 sampling analysis
The conventional nutrients contents such as each processing group feed measurement dry matter, crude protein.
2.2 growth performance
Test is responsible for by special messenger, is responsible for experimental animal raising, Cheng Chong, record.
Feed intake measures weekly.6:30 weighs on an empty stomach in the morning, measures feed intake, calculates separately that each processing group is per day to adopt
Appetite (ADFI), daily gain (ADG) and gain feed ratio (G/F).
The acquisition of 2.3 blood samples
The 5th, 10,15 day is tested respectively after feeding 2h, all individuals are stood through jugular vein blood collection 20ml, until naturally solidifying
Gu 3000rpm is centrifuged 10 minutes, serum is obtained, -20 degree save in case Biochemical Indices In Serum is analyzed.Biochemical Indices In Serum includes
Total protein, albumin, urea nitrogen, glucose, cholesterol and triglycerides analyze (Hitachi 7020) with automatic clinical chemistry analyzer.
2.5 statistical analysis
Data useMIXED program analysis (SAS Institute, 2000) in software.Test result is used flat
Mean and SEM are indicated.
3 interpretations of result
3.1 growth performance
As can be seen from Table 9, not having negative effect to beef cattle feed intake using sweet potato residue fermented feed, 2.8% is increased instead,
But difference is not significant (P < 0.05).Increasing for feed intake may be related with the palatability of feed is improved after sweet potato dregs fermentation.
Part rapeseed meal is substituted using sweet potato residue fermented feed and corn improves the daily gain of beef cattle, and addition bamboo shoot shell group is average
The high control group 5.0% of daily gain, and the weight gain of test group feeding than control group increases 2.1%, this explanation, when using sweet potato dregs
When fermented feed, the appetite of beef cattle can be improved, and also helpful to feed conversion rate, this also leads to increasing day by day for test group beef cattle
Weight and weight gain feeding are than being higher than control group.Therefore, beef cattle is grown using sweet potato residue fermented feed in cattle diet not negative
It influences.
Influence of the 9 sweet potato residue fermented feed of table to beef cattle growth performance
3.2 physiochemical indice
As shown in table 10, in daily ration using sweet potato residue fermented feed to the blood total protein of beef cattle, albumin, blood glucose and
The content of urea nitrogen has no significant effect (P > 0.05).The blood urea nitrogen (BUN) content difference of test group and control group beef cattle is not significant
Illustrate to have no adverse effect beef cattle nitrogen metabolism using sweet potato residue fermented feed in daily ration.In addition, test group and control group beef cattle
Blood triglyceride and total cholesterol level be also not significantly different, illustrate that the lipid-metabolism of beef cattle is not affected.
Influence of the fermentation sweet potato dreg fodder of table 10 to beef cattle physiochemical indice
* indicate that P < 0.05, NS indicate indifference.Shoulder mark difference lowercase letter indication difference of going together is significant (P < 0.05).
To sum up, substituting rapeseed meal and part corn using sweet potato residue fermented feed does not influence the growth performance of beef cattle.To beef cattle
Physiochemical indice also without adverse effect.Sweet potato residue fermented feed dosage can account for the 7% of daily ration.
Embodiment 2:
A kind of sweet potato residue fermented feed of ruminant, the fermented feed is that cud is inoculated in solid-state fermentation culture medium
Liquid post-fermentation obtains afterwards for 24 hours, and rumen fluid inoculum concentration is 8%；It include based on mass fraction in the solid-state fermentation culture medium
Following component: sweet potato dregs 45%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, urea 1%, surplus are water.The preparatory powder of sweet potato dregs
Partial size is broken within the scope of 0.5-2mm.
Embodiment 3:
A kind of sweet potato residue fermented feed of ruminant, the fermented feed is that cud is inoculated in solid-state fermentation culture medium
Liquid post-fermentation obtains afterwards for 24 hours, and rumen fluid inoculum concentration is 10%；It include based on mass fraction in the solid-state fermentation culture medium
Following component: sweet potato dregs 40%, potassium dihydrogen phosphate 0.8%, magnesium sulfate 0.5%, urea 2%, surplus are water.The preparatory powder of sweet potato dregs
Partial size is broken within the scope of 0.5-2mm.
Embodiment 4:
A kind of sweet potato residue fermented feed of ruminant, the fermented feed is that cud is inoculated in solid-state fermentation culture medium
It is obtained after liquid post-fermentation 26h, rumen fluid inoculum concentration is 8%；It include based on mass fraction in the solid-state fermentation culture medium
Following component: sweet potato dregs 50%, potassium dihydrogen phosphate 0.5%, magnesium sulfate 0.3%, urea 1%, surplus are water.The preparatory powder of sweet potato dregs
Partial size is broken within the scope of 0.5-2mm.
Embodiment 5-10:
A kind of sheep (beef cattle) feeding Diet Formula, formula raw material as described in table 11 are mixed.
Table 11 (unit: kg)
The present invention substitutes high-quality protein feed with sweet potato residue fermented feed, and the offal treatment for solving processing of farm products is asked
Topic, substantially reduces ruminant (ox, sheep etc.) aquaculture cost.Embodiment described above is that one kind of the invention is preferable
Scheme is not intended to limit the present invention in any form, on the premise of not exceeding the technical scheme recorded in the claims
There are also other variations and modifications.
Claims (6)
1. a kind of sweet potato residue fermented feed of ruminant, it is characterised in that: the fermented feed is in solid-state fermentation culture medium
Upper inoculation rumen fluid post-fermentation is derived above for 24 hours, and rumen fluid inoculum concentration is 7-10%；Include in the solid-state fermentation culture medium
Following component based on mass fraction: sweet potato dregs 40-50%, potassium dihydrogen phosphate 0.5-0.8%, magnesium sulfate 0.3-0.5%, urea 1% ~
3%, surplus is water.
2. a kind of preparation method of the sweet potato residue fermented feed of ruminant described in claim 1, it is characterised in that: described
Each raw material in solid-state fermentation culture medium is first dissolved in suitable quantity of water respectively, mixes in proportion after being sterilized separately, and supplies the water of formula ratio
Afterwards, the rumen fluid after being inoculated with impurity screening is cultivated more than for 24 hours in 30 DEG C in constant incubator.
3. preparation method according to claim 2, it is characterised in that: the incubation time is 24-26h.
4. a kind of application of sweet potato residue fermented feed described in claim 1 in sheep or beef cattle feeding, it is characterised in that: institute
The 6%-15% of sheep or cattle diet can be accounted for by stating sweet potato residue fermented feed dosage.
5. a kind of sheep feeds daily ration, it is characterised in that the formula includes the component of following mass fraction:
Sheep's hay 40-50%,
Green grass 17-20%,
Corn 16-18%,
Dregs of beans 5-6%,
Wheat bran 3-4%,
Salt 0.5-0.8%,
Calcium monohydrogen phosphate 0.5-0.8%,
Sweet potato residue fermented feed 6-15% described in claim 1.
6. a kind of beef cattle feeds daily ration, it is characterised in that the formula includes the component of following mass fraction:
Sheep's hay 20-30%,
Silage corn 18-38%,
Corn 24-26%,
Dregs of beans 6-8%,
Wheat bran 4-6%,
Salt 0.5-0.8%,
Calcium monohydrogen phosphate 0.5-0.8%,
Sweet potato residue fermented feed 6-15% described in claim 1.
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| CN103749967A (en) * | 2014-01-27 | 2014-04-30 | 中国农业科学院饲料研究所 | Sweet potato residue fermentation feed and preparation method thereof |
| CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
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2016
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| CN101965903A (en) * | 2010-08-17 | 2011-02-09 | 钱国英 | Preparation method of fermented vegetable protein |
| CN103371271A (en) * | 2012-04-30 | 2013-10-30 | 罗福仲 | Processing method for biological fermentation feed auxiliary material with sweet potato peel slag |
| CN102934736A (en) * | 2012-12-05 | 2013-02-20 | 蒋常德 | Method for preparing sweet potato skin/ sweet potato powder dreg fermented feed |
| CN103271224A (en) * | 2013-06-05 | 2013-09-04 | 吉首大学 | Method for producing single-cell protein feed by means of fermentation of sweet potato residues |
| CN103749967A (en) * | 2014-01-27 | 2014-04-30 | 中国农业科学院饲料研究所 | Sweet potato residue fermentation feed and preparation method thereof |
| CN104855686A (en) * | 2015-04-24 | 2015-08-26 | 中国科学院亚热带农业生态研究所 | A protein feed produced by fermenting sweet potato residues and a method to prolong shelf life thereof |
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