CN111493225A - Production method of rice protein residue biological protein feed - Google Patents
Production method of rice protein residue biological protein feed Download PDFInfo
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- CN111493225A CN111493225A CN202010278919.2A CN202010278919A CN111493225A CN 111493225 A CN111493225 A CN 111493225A CN 202010278919 A CN202010278919 A CN 202010278919A CN 111493225 A CN111493225 A CN 111493225A
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- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 67
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 67
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 47
- 235000009566 rice Nutrition 0.000 title claims abstract description 47
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 19
- 240000007594 Oryza sativa Species 0.000 title abstract 3
- 238000000855 fermentation Methods 0.000 claims abstract description 41
- 230000004151 fermentation Effects 0.000 claims abstract description 41
- 239000000463 material Substances 0.000 claims abstract description 23
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 241000894006 Bacteria Species 0.000 claims abstract description 14
- 238000010563 solid-state fermentation Methods 0.000 claims abstract description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 11
- 238000001035 drying Methods 0.000 claims abstract description 11
- 239000000203 mixture Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 9
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 8
- 235000013555 soy sauce Nutrition 0.000 claims abstract description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 7
- 238000007873 sieving Methods 0.000 claims abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 4
- 241000209094 Oryza Species 0.000 claims description 44
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 20
- 235000014655 lactic acid Nutrition 0.000 claims description 10
- 239000004310 lactic acid Substances 0.000 claims description 10
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 7
- 241000235342 Saccharomycetes Species 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 3
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 2
- 241000193755 Bacillus cereus Species 0.000 claims description 2
- 241000193749 Bacillus coagulans Species 0.000 claims description 2
- 241000194108 Bacillus licheniformis Species 0.000 claims description 2
- 241000194107 Bacillus megaterium Species 0.000 claims description 2
- 241000194103 Bacillus pumilus Species 0.000 claims description 2
- 244000063299 Bacillus subtilis Species 0.000 claims description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 2
- 241000186000 Bifidobacterium Species 0.000 claims description 2
- 241000193764 Brevibacillus brevis Species 0.000 claims description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 claims description 2
- 241000194032 Enterococcus faecalis Species 0.000 claims description 2
- 241000194031 Enterococcus faecium Species 0.000 claims description 2
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 2
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 2
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims description 2
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 2
- 241000191996 Pediococcus pentosaceus Species 0.000 claims description 2
- 229940054340 bacillus coagulans Drugs 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 229940032049 enterococcus faecalis Drugs 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 2
- 235000015067 sauces Nutrition 0.000 claims description 2
- 241000101515 Staphylotrichum Species 0.000 claims 1
- 239000002893 slag Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 11
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 244000144972 livestock Species 0.000 abstract description 5
- 244000005700 microbiome Species 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 230000029087 digestion Effects 0.000 abstract description 3
- 241000186660 Lactobacillus Species 0.000 abstract description 2
- 150000001720 carbohydrates Chemical class 0.000 abstract description 2
- 239000001913 cellulose Substances 0.000 abstract description 2
- 229920002678 cellulose Polymers 0.000 abstract description 2
- 244000005709 gut microbiome Species 0.000 abstract description 2
- 230000007413 intestinal health Effects 0.000 abstract description 2
- 229940039696 lactobacillus Drugs 0.000 abstract description 2
- 235000018102 proteins Nutrition 0.000 description 50
- 235000019750 Crude protein Nutrition 0.000 description 4
- 235000019764 Soybean Meal Nutrition 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 239000000835 fiber Substances 0.000 description 4
- 239000004455 soybean meal Substances 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010022998 Irritability Diseases 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001236817 Paecilomyces <Clavicipitaceae> Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 208000030961 allergic reaction Diseases 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 1
- 235000013923 monosodium glutamate Nutrition 0.000 description 1
- 239000004223 monosodium glutamate Substances 0.000 description 1
- 231100000344 non-irritating Toxicity 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/137—Delbrueckii
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
A production method of rice protein residue biological protein feed, belonging to the technical field of livestock feed. The method comprises the following steps: (1) respectively crushing and sieving raw materials of rice protein residue, bean pulp, bran and soy sauce in a tank, and uniformly stirring to obtain a mixture; (2) dissolving in water, adding mixed fermentation microbial inoculum containing lactobacillus, yeast and bacillus, and performing solid state fermentation to obtain fermented wet material; (3) and (5) quickly drying to obtain a protein feed finished product. The invention adopts a plurality of beneficial bacteria to ferment the material, and the carbohydrate, macromolecular protein and cellulose substances in the material are greatly degraded under the fermentation action of a plurality of microorganisms, so that the protein quality is obviously improved, the intestinal microflora of animals is improved, the intestinal health of the animals is improved, the digestion capacity and the feed utilization efficiency of the animals are improved, the production performance of the animals is improved, the morbidity is reduced, and the sustainable development of the livestock industry is promoted.
Description
Technical Field
The invention belongs to the technical field of livestock feed, and particularly relates to a production method of rice protein residue biological protein feed.
Background
Many products produced by taking rice as raw materials, such as monosodium glutamate, alcohol, lactic acid, starch sugar and the like, and the problem of value-added utilization of rice grains or rice residues which are byproducts of the saccharified rice is a difficult problem which is difficult to solve by enterprises. The protein content of the rice protein residue is higher than that of bean pulp, rapeseed pulp and the like, and the rice protein residue is rich in amino acids essential to human bodies, particularly the lysine content is higher than that of other grains. The rice protein has high biological value, occupies the first place in grain crops, and has a nutritive value comparable with that of eggs and milk. In addition, the rice protein is low in antigenicity and does not cause allergic reaction. Because the rice protein has low irritability, no pigment interference, soft and non-irritating taste and high nutritional value, and the rice protein residue has rich resources and low price, the beneficial microorganisms are utilized to fully ferment the rice protein, and the production of animal protein feed has important economic value.
The protein raw material is prepared by microbial solid state fermentation, so that the digestion and absorption of animals can be improved, the immunity of the animals can be enhanced, the meat quality can be improved, and the like. The fermented feed converts protein and starch substances contained in the protein raw material into micromolecular peptide substances, amino acids, fatty acids, glucose and other micromolecular substances through the self metabolism of microorganisms, is easy to digest and absorb by livestock and poultry, and has no toxic or side effect. The microbial fermentation solid feed has good development prospect and will become a great trend.
Disclosure of Invention
In view of the problems in the prior art, the invention aims to design and provide a production method of a rice protein residue biological protein feed. The invention relates to a protein fermentation feed prepared by taking rice protein residue, soybean meal, bran and soy sauce lees as raw materials and utilizing microbial solid state fermentation, which has simple process and is convenient for industrial production. The content of protein in the rice protein residue feed is improved through the fermentation of microorganisms, and the rice protein residue feed can be widely used for preparing various animal feeds.
A production method of rice protein residue biological protein feed is characterized by comprising the following steps:
(1) respectively crushing the rice protein residue, the bean pulp, the bran and the soy sauce serving as raw materials, sieving the crushed materials with a 60-mesh sieve, and uniformly stirring the crushed materials in a stirring tank to obtain a mixture;
(2) adding a mixed fermentation microbial inoculum containing lactobacillus, saccharomycetes and bacillus into the mixture obtained in the step (1), keeping the humidity at 50-60%, and performing solid state fermentation after uniformly stirring to obtain a fermented wet material;
(3) and (3) quickly drying the fermented wet material obtained in the step (2) to obtain a protein feed finished product.
The production method of the rice protein residue biological protein feed is characterized in that the raw materials in the step (1) are mixed according to the following ratio: 65-85 parts of rice protein residues, 10-15 parts of bean pulp, 15-20 parts of bran and 10-15 parts of sauce oil tanks.
The production method of the rice protein residue biological protein feed is characterized in that the yeast in the fermentation bacteria agent in the step (2): lactic acid bacteria: the mass ratio of the bacillus is 4-2:1:1, and the inoculation amount of the fermentation inoculum is 8-12%.
The production method of the rice protein residue biological protein feed is characterized in that in the step (2), the lactic acid bacteria comprise bifidobacterium, enterococcus faecalis, enterococcus faecium, pediococcus pentosaceus, lactobacillus acidophilus, lactobacillus plantarum or lactobacillus delbrueckii, the yeast comprises saccharomyces cerevisiae, candida utilis or paecilomyces fibuligerus, and the bacillus comprises bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus cereus, bacillus megaterium, bacillus amyloliquefaciens, bacillus pumilus or bacillus brevis.
The production method of the rice protein residue biological protein feed is characterized in that the solid state fermentation conditions in the step (2) are as follows: the fermentation temperature is 30-35 ℃, and the fermentation time is 36-54 h.
The production method of the rice protein residue biological protein feed is characterized in that the solid-state fermentation equipment in the step (2) is a chain belt type automatic fermentation bed.
The invention has the following beneficial effects:
(1) the invention adopts the chain belt type automatic fermentation bed, has automatic control in the whole process, reduces labor force and reduces the influence of human factors. The ventilation is good in the whole fermentation process, the fermentation is uniform and thorough, and the quantity of the generated beneficial bacteria is large. The fermentation process adopts automatic material turning, the fermentation uniformity is high, the fermentation controllable degree is high (temperature and ventilation), and the product quality is stable.
(2) The invention adopts a plurality of beneficial bacteria to ferment the material, and the carbohydrate, macromolecular protein and cellulose substances in the material are greatly degraded under the fermentation action of a plurality of microorganisms, so that the protein quality is obviously improved, the intestinal microflora of animals is improved, the intestinal health of the animals is improved, the digestion capacity and the feed utilization efficiency of the animals are improved, the production performance of the animals is improved, the morbidity is reduced, and the sustainable development of the livestock industry is promoted.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1:
(1) preparing materials: weighing 65 kg of rice protein residue, 10 kg of bean pulp, 15kg of bran and 10 kg of soy sauce residue, crushing, sieving with a 60-mesh sieve, and putting into a stirring pool to stir uniformly.
(2) And (2) fermentation, namely dissolving in 60L water, adding a mixed fermentation microbial inoculum containing 4kg of lactic acid bacteria, 2kg of saccharomycetes and 2kg of bacillus, inoculating 8%, keeping the humidity at 50-60%, uniformly stirring, moving the mixture to a chain belt type automatic fermentation bed for solid state fermentation, controlling the fermentation temperature at 32-35 ℃, and fermenting for 48 hours to obtain a fermented wet material, wherein the inoculation is uniform during the solid state fermentation, the material is turned over in time to avoid the temperature from rising too fast, and the ventilation and the spreading thickness are uniform during the fermentation process.
(3) Drying and quickly drying to obtain the rice protein residue fermented feed.
The detection proves that the rice protein residue fermented feed contains 56.84% of crude protein, 5.74% of crude fiber and 3.80% of water.
Example 2:
(1) preparing materials: weighing 85 kg of rice protein residue, 15kg of soybean meal, 20kg of bran and 15kg of soy sauce residue, crushing, sieving with a 60-mesh sieve, and putting into a stirring pool to stir uniformly.
(2) And (2) fermentation, namely dissolving the mixture in 80L water, adding a mixed fermentation microbial inoculum containing 8.1kg of lactic acid bacteria, 2.7kg of saccharomycetes and 2.7kg of bacillus, inoculating 10 percent of the mixture, keeping the humidity at 50-60 percent, uniformly stirring, moving the mixture to a chain belt type automatic fermentation bed for solid state fermentation, controlling the fermentation temperature at 32-35 ℃, and fermenting for 54 hours to obtain a fermented wet material.
(3) Drying and quickly drying to obtain the rice protein residue fermented feed.
Through detection, the rice protein residue fermented feed contains 55.84% of crude protein, 4.73% of crude fiber and 3.91% of water.
Example 3:
(1) preparing materials: weighing 70 kg of rice protein residue, 15kg of soybean meal, 15kg of bran and 15kg of soy sauce residue, crushing, sieving with a 60-mesh sieve, and putting into a stirring pool to stir uniformly.
(2) Dissolving in 69L water, adding a mixed fermentation microbial inoculum containing 4.6kg of lactic acid bacteria, 2.3kg of yeast and 2.3kg of bacillus, inoculating 12 percent of the mixed fermentation microbial inoculum, keeping the humidity at 50-60 percent, uniformly stirring, moving the mixed fermentation microbial inoculum to a chain belt type automatic fermentation bed for solid state fermentation, controlling the fermentation temperature at 32-35 ℃, and fermenting for 36 hours to obtain a fermented wet material.
(3) Drying and quickly drying to obtain the rice protein residue fermented feed.
Through detection, the rice protein residue fermented feed contains 54.84% of crude protein, 4.14% of crude fiber and 3.59% of water.
Example 4:
(1) preparing materials: weighing 80 kg of rice protein residue, 13 kg of soybean meal, 15kg of bran and 12 kg of soy sauce residue, crushing, sieving with a 60-mesh sieve, and putting into a stirring pool to stir uniformly.
(2) Dissolving in 75L water, adding a mixed fermentation microbial inoculum containing 8kg of lactic acid bacteria, 2kg of saccharomycetes and 2kg of bacillus, inoculating 12%, keeping the humidity at 50-60%, uniformly stirring, moving the mixture to a chain belt type automatic fermentation bed for solid state fermentation, controlling the fermentation temperature at 32-35 ℃, and fermenting for 48 hours to obtain a fermented wet material.
(3) Drying and quickly drying to obtain the rice protein residue fermented feed.
The detection proves that the rice protein residue fermented feed contains 58.84% of crude protein, 4.74% of crude fiber and 3.25% of water.
Claims (6)
1. A production method of rice protein residue biological protein feed is characterized by comprising the following steps:
(1) respectively crushing the rice protein residue, the bean pulp, the bran and the soy sauce serving as raw materials, sieving the crushed materials with a 60-mesh sieve, and uniformly stirring the crushed materials in a stirring tank to obtain a mixture;
(2) dissolving the mixture obtained in the step (1) in water, adding a mixed fermentation microbial inoculum containing lactic acid bacteria, saccharomycetes and bacillus, keeping the humidity at 50-60%, uniformly stirring, and performing solid state fermentation to obtain a fermented wet material;
(3) and (3) quickly drying the fermented wet material obtained in the step (2) to obtain a protein feed finished product.
2. The production method of the rice protein residue bioprotein feed as claimed in claim 1, wherein the raw materials in the step (1) are in the following ratio: 65-85 parts of rice protein residues, 10-15 parts of bean pulp, 15-20 parts of bran and 10-15 parts of sauce oil tanks.
3. The method for producing the rice protein slag bioprotein feed according to claim 1, wherein yeasts in the fermentation bacteria agent in the step (2): lactic acid bacteria: the mass ratio of the bacillus is 4-2:1:1, and the inoculation amount of the fermentation inoculum is 8-12%.
4. The method of claim 1, wherein the lactic acid bacteria in step (2) comprise Bifidobacterium, enterococcus faecalis, enterococcus faecium, Pediococcus pentosaceus, Lactobacillus acidophilus, Lactobacillus plantarum, or Lactobacillus delbrueckii, the yeast comprises Saccharomyces cerevisiae, Candida utilis, or Staphylotrichum dunnii, and the Bacillus comprises Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans, Bacillus cereus, Bacillus megaterium, Bacillus amyloliquefaciens, Bacillus pumilus, or Bacillus brevis.
5. The method for producing a rice protein residue bioprotein feed according to claim 1, wherein the solid state fermentation conditions in the step (2) are as follows: the fermentation temperature is 30-35 ℃, and the fermentation time is 36-54 h.
6. The method for producing a rice protein residue bioprotein feed of claim 1, wherein the solid state fermentation device in the step (2) is a chain belt type automatic fermentation bed.
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| CN202010278919.2A CN111493225A (en) | 2020-04-10 | 2020-04-10 | Production method of rice protein residue biological protein feed |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715780A (en) * | 2020-12-23 | 2021-04-30 | 武汉轻工大学 | Composite zymophyte, and preparation method and application of broad bean meal fermented product |
| CN114343060A (en) * | 2021-12-30 | 2022-04-15 | 河南金丹乳酸科技股份有限公司 | Process for improving protein content in thallus residue in lactic acid production |
| CN115843921A (en) * | 2023-02-14 | 2023-03-28 | 成都铁骑力士饲料有限公司 | Biological pretreatment method for reducing mildew of wet residue in feed application |
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| CN102934737A (en) * | 2012-12-10 | 2013-02-20 | 广西高源淀粉有限公司 | Preparation method of protein feed |
| CN103931885A (en) * | 2014-04-01 | 2014-07-23 | 安徽五粮泰生物工程股份有限公司 | Preparation method of acidified small-peptide protein feed |
| CN109287865A (en) * | 2018-10-31 | 2019-02-01 | 宁波中瑞生物科技有限公司 | A kind of composite fermentation protein feed and preparation method thereof |
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| CN114343060B (en) * | 2021-12-30 | 2023-07-25 | 河南金丹乳酸科技股份有限公司 | Process for increasing protein content in thallus slag in lactic acid production |
| CN115843921A (en) * | 2023-02-14 | 2023-03-28 | 成都铁骑力士饲料有限公司 | Biological pretreatment method for reducing mildew of wet residue in feed application |
| CN115843921B (en) * | 2023-02-14 | 2023-07-04 | 成都铁骑力士饲料有限公司 | Biological pretreatment method for reducing mildew of wet residues in feed application |
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