CN112998120A - Preparation method of jack fruit seed feed - Google Patents
Preparation method of jack fruit seed feed Download PDFInfo
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- CN112998120A CN112998120A CN202110344918.8A CN202110344918A CN112998120A CN 112998120 A CN112998120 A CN 112998120A CN 202110344918 A CN202110344918 A CN 202110344918A CN 112998120 A CN112998120 A CN 112998120A
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- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 4
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- 241000194032 Enterococcus faecalis Species 0.000 claims description 4
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- 241000186015 Bifidobacterium longum subsp. infantis Species 0.000 claims description 2
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 claims description 2
- 241001106597 Enterococcus lactis Species 0.000 claims description 2
- 241000235649 Kluyveromyces Species 0.000 claims description 2
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- 241000186840 Lactobacillus fermentum Species 0.000 claims description 2
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- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 2
- 229940017800 lactobacillus casei Drugs 0.000 claims description 2
- 229940012969 lactobacillus fermentum Drugs 0.000 claims description 2
- 229940001882 lactobacillus reuteri Drugs 0.000 claims description 2
- 229960002181 saccharomyces boulardii Drugs 0.000 claims description 2
- 238000010521 absorption reaction Methods 0.000 abstract description 11
- 150000001413 amino acids Chemical class 0.000 abstract description 8
- 239000007788 liquid Substances 0.000 abstract description 7
- 239000006227 byproduct Substances 0.000 abstract description 6
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- 102000004169 proteins and genes Human genes 0.000 abstract description 6
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 abstract description 5
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 abstract description 5
- 229920002472 Starch Polymers 0.000 abstract description 5
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- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 230000001079 digestive effect Effects 0.000 description 6
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- 244000144977 poultry Species 0.000 description 2
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- 230000000975 bioactive effect Effects 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
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- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of feed processing, in particular to a preparation method of jackfruit seed feed. The preparation method of the jack fruit seed feed is characterized by comprising the following steps: s1, pretreating jackfruit seeds; s2, enzymolysis; s3, adding a leavening agent; s4, fermenting; s5, preparing a wet fermented feed; s6, preparing the dry fermented feed. Compared with the prior art, the byproducts of fruit processing are reasonably utilized, the starch is converted into maltose and other micromolecular saccharides after the jackfruit seeds are subjected to enzymolysis, the protein is partially decomposed into peptide and amino acid, the composite bacterial strain liquid fermentation and digestion absorption are suitable, and the finally prepared feed is rich in nutrition and promotes digestion and absorption.
Description
Technical Field
The invention relates to the technical field of feed processing, in particular to a preparation method of jackfruit seed feed.
Background
The jackfruit is a typical tropical fruit, the seed of the jackfruit is one of byproducts, the yield per year is considerable, the protein content is high, the amino acid composition is reasonable, 8 essential amino acids required by a human body are contained, and the jackfruit belongs to complete protein. The proportion of unsaturated fatty acid in the fatty acid composition of the jackfruit seed powder is high, the content of essential fatty acid is high, and the jackfruit seed powder has good nutritional value.
The fermented feed takes microorganisms and compound enzymes as biological feed starter strains, and raw materials of the feed are converted into microbial mycoprotein, bioactive small peptide amino acids, microbial active probiotics and compound enzymes which are integrated into a biological fermented feed. The product not only can make up amino acid which is easy to be lacked in conventional feed, but also can quickly convert the nutrient components of other raw feed materials, thereby achieving the effect of enhancing digestion, absorption and utilization.
Therefore, the preparation method of the jack fruit seed feed reasonably utilizes the byproducts of fruit processing, the jack fruit seeds are subjected to enzymolysis, starch is converted into maltose and other small molecular saccharides, and protein is partially decomposed into peptide and amino acid, so that the jack fruit seed feed is suitable for liquid fermentation and digestive absorption of various composite strains, the finally prepared feed is rich in nutrition, and the digestive absorption effect is enhanced.
Disclosure of Invention
The invention provides a preparation method of jack fruit seed feed for overcoming the defects of the prior art, which reasonably utilizes byproducts of fruit processing, starch is converted into maltose and other micromolecular carbohydrate substances after the jack fruit seeds are subjected to enzymolysis, and protein is partially decomposed into peptide and amino acid, so that the preparation method is suitable for liquid fermentation and digestive absorption of various composite strains, and the finally prepared feed is rich in nutrition and enhances the digestive absorption effect.
In order to realize the aim, the preparation method of the jack fruit seed feed is designed, and is characterized by comprising the following steps:
s1, pretreatment of jack fruit seeds: respectively cleaning and crushing 800 parts of 500-plus jackfruit seeds, adding water for pulping, grinding, adding auxiliary materials, sterilizing by steam, drying by hot air and adjusting the water content of the mixture to 50-70%;
s2, enzymolysis: cooling the mixture obtained in the step S1 to 40-60 ℃, adding 0.1-1 part of enzyme preparation, uniformly stirring, performing constant-temperature enzymolysis for 30-180min to prepare coarse feed, and cooling to the fermentation temperature of 25-45 ℃;
s3, adding a leavening agent: adding 10-40 parts of direct vat starter to the coarse fodder obtained in step S2;
s4, fermentation: inoculating starter, stirring, fermenting at 25-42 deg.C for 2-15 days, and stopping fermentation until pH is not more than 5.0;
s5, preparing a wet fermentation feed: directly sealing and packaging the fermented feed in the step S4 to prepare wet fermented feed;
s6, preparing dry fermented feed: and (5) drying the feed fermented in the step (S4) until the moisture content is less than 8%, crushing, sealing and packaging to prepare the dry fermented feed.
The auxiliary materials in the step S1 include one or more of soybean meal, crushed corn stalks and bran.
The enzyme preparation comprises one or more of amylase, cellulase and protease.
The direct vat set starter comprises one or more of lactobacillus, bifidobacterium, bacillus and yeast.
The direct vat set starter also comprises one or more of trichoderma viride, aspergillus niger, aspergillus oryzae and bacillus butyricum.
The lactobacillus comprises one or more of enterococcus faecium, enterococcus faecalis, enterococcus lactis, pediococcus acidilactici, pediococcus pentosaceus, lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus buchneri, lactobacillus reuteri, lactobacillus delbrueckii subsp lactis, lactobacillus fermentum and lactobacillus cellobiose.
The Bifidobacterium includes one or more of Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescentis, and Bifidobacterium animalis.
The bacillus comprises one or more of bacillus coagulans, bacillus subtilis, bacillus licheniformis, bacillus megaterium and bacillus pumilus.
The yeast comprises one or more of candida utilis, saccharomyces cerevisiae, saccharomyces boulardii and kluyveromyces.
The wet fermented feed is stored in a shade place, the shelf life is 60 days, and the dry fermented feed is stored in a shade place, and the shelf life is 1 year.
Compared with the prior art, the method reasonably utilizes the byproducts of fruit processing, the starch is converted into maltose and other micromolecular saccharides after the jackfruit seeds are subjected to enzymolysis, the protein is partially decomposed into peptide and amino acid, the method is suitable for liquid fermentation and digestive absorption of various composite strains, and finally the prepared feed is rich in nutrition and promotes digestive absorption.
Detailed Description
The first embodiment is as follows:
the embodiment is a method for preparing wet fermented feed by using jack fruit seeds, and the method specifically comprises the following steps:
s1, pretreatment of jack fruit seeds: respectively cleaning 800 parts of jack fruit seeds, grinding, adding 400 parts of water for pulping, grinding, adding 100 parts of soybean meal and 100 parts of crushed corn straws, sterilizing with steam at 115 ℃ for 5min, and drying with hot air until the water content of the mixture is 70%;
s2, enzymolysis: cooling the mixture obtained in the step S1 to 60 ℃, adding 0.1 part of enzyme preparation, uniformly stirring, performing constant-temperature enzymolysis for 30min to prepare coarse feed, and cooling to the fermentation temperature of 25 +/-2 ℃;
s3, adding a leavening agent: adding 40 parts of direct vat starter to the coarse fodder obtained in step S2;
s4, fermentation: inoculating the starter, stirring, fermenting at 25 + -2 deg.C for 15 days, and stopping fermentation until pH is not more than 5.0, wherein the material liquid has obvious fermentation flavor;
s5, preparing a wet fermentation feed: and (4) directly sealing and packaging the fermented feed in the step (S4) to prepare the wet fermented feed.
The enzyme preparation of the embodiment comprises 5: 2: 3 is amylase, cellulase and protease.
The direct vat set starter of the embodiment is composed of enterococcus faecium, enterococcus faecalis, lactobacillus plantarum, bifidobacterium longum, bacillus subtilis, bacillus licheniformis, candida utilis and saccharomyces cerevisiae which are evenly distributed according to parts by mass.
The wet fermented feed obtained in this example was stored in the shade for a shelf life of 60 days and could be used for feeding pigs, cattle and poultry. The composition can be used alone or mixed with other feeds.
Example two:
the embodiment is a method for preparing dry fermented feed by using jack fruit seeds, and the method specifically comprises the following steps:
s1, pretreatment of jack fruit seeds: respectively cleaning 500 parts of jack fruit seeds, grinding, adding 500 parts of water, pulping, grinding, adding 90 parts of soybean meal and 10 parts of bran, sterilizing with steam at 115 ℃ for 5min, and drying with hot air until the water content of the mixture is 50%;
s2, enzymolysis: cooling the mixture obtained in the step S1 to 40 ℃, adding 1 part of enzyme preparation, uniformly stirring, performing constant-temperature enzymolysis for 180min to prepare coarse feed, and cooling to the fermentation temperature of 42 +/-2 ℃;
s3, adding a leavening agent: adding 10 parts of direct vat starter to the coarse fodder obtained in step S2;
s4, fermentation: inoculating the starter, stirring, fermenting at 42 + -2 deg.C for 2 days, and stopping fermentation until pH is not more than 5.0, wherein the material liquid has obvious fermentation flavor;
s5, preparing dry fermented feed: and (5) drying the feed fermented in the step (S4) until the moisture content is less than 8%, crushing, sealing and packaging to prepare the dry fermented feed.
The enzyme preparation of the present example comprises 4: 5: amylase, cellulase and protease of formula 1.
The direct vat set starter of the embodiment is composed of enterococcus faecium, enterococcus faecalis, lactobacillus plantarum, lactobacillus delbrueckii subsp lactis, bifidobacterium adolescentis, bacillus coagulans, bacillus subtilis, candida utilis, saccharomyces cerevisiae, aspergillus oryzae and bacillus butyricum which are distributed evenly according to parts by weight.
The dry fermented feed obtained in this example was stored in the shade for 1 year and could be used for feeding pigs, cattle and poultry. The composition can be used alone or mixed with other feeds.
According to the invention, by-products of fruit processing are reasonably utilized, the starch is converted into maltose and other small molecular saccharides after the jackfruit seeds are subjected to enzymolysis, the protein is partially decomposed into peptide and amino acid, the composite bacterial strain liquid fermentation and digestion absorption are suitable, and the finally prepared feed is rich in nutrition and promotes digestion and absorption.
Claims (10)
1. The preparation method of the jack fruit seed feed is characterized by comprising the following steps:
s1, pretreatment of jack fruit seeds: respectively cleaning and crushing 800 parts of 500-plus jackfruit seeds, adding water for pulping, grinding, adding auxiliary materials, sterilizing by steam, drying by hot air and adjusting the water content of the mixture to 50-70%;
s2, enzymolysis: cooling the mixture obtained in the step S1 to 40-60 ℃, adding 0.1-1 part of enzyme preparation, uniformly stirring, performing constant-temperature enzymolysis for 30-180min to prepare coarse feed, and cooling to the fermentation temperature of 25-45 ℃;
s3, adding a leavening agent: adding 10-40 parts of direct vat starter to the coarse fodder obtained in step S2;
s4, fermentation: inoculating starter, stirring, fermenting at 25-42 deg.C for 2-15 days, and stopping fermentation until pH is not more than 5.0;
s5, preparing a wet fermentation feed: directly sealing and packaging the fermented feed in the step S4 to prepare wet fermented feed;
s6, preparing dry fermented feed: and (5) drying the feed fermented in the step (S4) until the moisture content is less than 8%, crushing, sealing and packaging to prepare the dry fermented feed.
2. The method for preparing the jack fruit seed feed according to claim 1, wherein the method comprises the following steps: the auxiliary materials in the step S1 include one or more of soybean meal, crushed corn stalks and bran.
3. The method for preparing the jack fruit seed feed according to claim 1, wherein the method comprises the following steps: the enzyme preparation comprises one or more of amylase, cellulase and protease.
4. The method for preparing the jack fruit seed feed according to claim 1, wherein the method comprises the following steps: the direct vat set starter comprises one or more of lactobacillus, bifidobacterium, bacillus and yeast.
5. The method for preparing the jack fruit seed feed according to claim 4, wherein the method comprises the following steps: the direct vat set starter also comprises one or more of trichoderma viride, aspergillus niger, aspergillus oryzae and bacillus butyricum.
6. The method for preparing the jack fruit seed feed according to claim 4, wherein the method comprises the following steps: the lactobacillus comprises one or more of enterococcus faecium, enterococcus faecalis, enterococcus lactis, pediococcus acidilactici, pediococcus pentosaceus, lactobacillus plantarum, lactobacillus acidophilus, lactobacillus casei, lactobacillus buchneri, lactobacillus reuteri, lactobacillus delbrueckii subsp lactis, lactobacillus fermentum and lactobacillus cellobiose.
7. The method for preparing the jack fruit seed feed according to claim 4, wherein the method comprises the following steps: the Bifidobacterium includes one or more of Bifidobacterium infantis, Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium adolescentis, and Bifidobacterium animalis.
8. The method for preparing the jack fruit seed feed according to claim 4, wherein the method comprises the following steps: the bacillus comprises one or more of bacillus coagulans, bacillus subtilis, bacillus licheniformis, bacillus megaterium and bacillus pumilus.
9. The method for preparing the jack fruit seed feed according to claim 4, wherein the method comprises the following steps: the yeast comprises one or more of candida utilis, saccharomyces cerevisiae, saccharomyces boulardii and kluyveromyces.
10. The method for preparing the jack fruit seed feed according to claim 1, wherein the method comprises the following steps: the wet fermented feed is stored in a shade place, the shelf life is 60 days, and the dry fermented feed is stored in a shade place, and the shelf life is 1 year.
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PCT/CN2021/125681 WO2022205856A1 (en) | 2021-03-31 | 2021-10-22 | Method for preparing jackfruit seed feed |
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WO2022205856A1 (en) * | 2021-03-31 | 2022-10-06 | 润盈生物工程(上海)有限公司 | Method for preparing jackfruit seed feed |
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CN111820376A (en) * | 2020-07-29 | 2020-10-27 | 润盈生物工程(上海)有限公司 | Preparation method of probiotic fermented lupin fermented product |
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CN112998120A (en) * | 2021-03-31 | 2021-06-22 | 润盈生物工程(上海)有限公司 | Preparation method of jack fruit seed feed |
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WO2022205856A1 (en) * | 2021-03-31 | 2022-10-06 | 润盈生物工程(上海)有限公司 | Method for preparing jackfruit seed feed |
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