CN103652328B - Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste - Google Patents
Method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste Download PDFInfo
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- CN103652328B CN103652328B CN201310675441.7A CN201310675441A CN103652328B CN 103652328 B CN103652328 B CN 103652328B CN 201310675441 A CN201310675441 A CN 201310675441A CN 103652328 B CN103652328 B CN 103652328B
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Abstract
The invention relates to a method for producing high-quality high-protein feed through mixed strain solid-state fermentation of rice dregs and manioc waste. The method comprises the following steps: (1) preparation of liquid seed: culturing bacillus subtilis with a nutrient broth liquid medium, culturing aspergillus niger and beer yeast with a potato glucose medium respectively, and mixing the cultured strains aspergillus niger, beer yeast and bacillus subtilis at a volume ratio to obtain the liquid seed; (2) proportionally mixing the rice dregs (dry basis) and manioc waste (dry basis), adding magnesium sulfate, dipotassium phosphate and water, mixing uniformly and inoculating the liquid seed, and culturing for 5-7 days at 28-32 DEG C; (3) drying the fermented material at 40-60 DEG C and grinding to obtain high-protein feed. The method provided by the invention increases the feed value of the rice dregs and manioc waste, and improves the palatability of manioc waste as feed so that the manioc waste is easy to absorb by animals; meanwhile, the method also can improve the effective microbial community in the intestinal tracts of the animals, and has the beneficial effect of improving the immunity and disease resistance of the animals.
Description
Technical field
The present invention relates to a kind of preparation method of protein feed, particularly a kind of method of mixed strains solid fermented rice residue and manioc waste production high-quality high protein feed.
Background technology
Rice residue take rice as the accessory substance that starch sugar produced by raw material, and often consume 10 tons of rice aborning and just produce 1 ton of dry rice residue, rich in protein in rice residue, up to more than 60%.The rice residue in starch sugar refinery is sold mainly as single feed at present, and product homogeneity is serious, added value is lower.Manioc waste take cassava as discarded object after raw material produces starch or alcohol, and wherein non-nitrogen compounds content reaches more than 75%, and main component is starch and crude fibre, and wherein starch accounts for about 50% of mass fraction, but protein content is low, only about 3%.Manioc waste is commonly used to produce fertilizer, feed, culturing edible fungus, fermentation methane, as fuel etc., also have the report of its manufacture order cell protein of research and utilization and biological feedstuff aspect.There are no the report adopting fermented by mixed bacterium rice residue and manioc waste to produce high protein feed.
Summary of the invention
The technical problem to be solved in the present invention is: a kind of method providing mixed strains solid fermented rice residue and manioc waste production high-quality high protein feed, adopt this method to produce its protein quality content of feed obtained and can reach 50 ~ 75%, invention increases the feeding value of rice residue and manioc waste in addition, improve the palatability of manioc waste as feed, be easy to animal absorb, profitable strain in animal intestinal can be improved again simultaneously, there is the beneficial effect improving animal immunizing power and premunition.
The technical scheme solved the problems of the technologies described above is: a kind of method of mixed strains solid fermented rice residue and manioc waste production high-quality high protein feed, comprises the following steps:
(1) the preparation of liquid seeds: cultivate bacillus subtilis 22-26h based on 35-40 DEG C by nutrient broth Liquid Culture, cultivate aspergillus niger 3-5d with potato dextrose medium respectively at 25-30 DEG C, cultivate brewer's yeast 2-3d in 25-30 DEG C, cultured bacterial classification is mixed and made into liquid seeds with the ratio of volume ratio Hei Qu Mei ︰ Pi wine Jiao Mu ︰ bacillus subtilis=1.5-2.5 ︰ 1.5-2.5 ︰ 1;
(2) be obtain mixture after 0.5-2 ︰ 1 mixes in mass ratio by dry rice residue and dry manioc waste, then the magnesium sulfate of mixture quality 0.1-0.3%, the dipotassium hydrogen phosphate of 0.3-0.5% and the water of 25-35% is added, liquid seeds is inoculated, in 28 DEG C-32 DEG C bottom fermentation 5-7d by the 8-12% of dry rice residue and dry manioc waste mixture quality after mixing;
(3) pulverize after the material after fermentation being dried in 40 ~ 60 DEG C, get product.
Described finished product is the high protein feed that protein quality content reaches 50 ~ 75%.
Described dry rice residue and the water content of dry manioc waste are lower than 10%.
The preparation method of described potato dextrose medium is: after potato being cleaned peeling stripping and slicing, take 200g and add water boil 20min, by eight layers of filtered through gauze, add 20g glucose in filtrate, then add water to 1000mL, use triangular flask packing, 121 DEG C of sterilizing 20min, for subsequent use after cooling.
The present invention utilizes the feature that rice residue protein content is high, manioc waste carbohydrate content is high, rice residue and manioc waste are combined by fermentation method production high-quality high protein feed, expand existing usage and purposes, can further improve the added value of rice residue and manioc waste.
The present invention adopts rice residue and manioc waste to mix raw material as microorganism solid fermentation, material in simple rice residue sweat is loose not, the shortcoming of hypoventilation to make up can to utilize the high feature of manioc waste crude fiber content on the one hand, and in rice residue, rich in protein can provide nitrogenous source for the sweat of manioc waste on the other hand.
The present invention adopts mixed strains solid fermented rice residue and manioc waste mixture, because can carry out collaborative alternate fermentation between multiple bacterial classification in mixed strain fermentation process, more effective than single strain fermentation.Mixed bacteria selects saccharomycete, mould and bacillus, and advantage is: saccharomycete can secrete multiple hydrolase, is rich in B family vitamin, and can produce the fissional active material of promotion, has the effect of enriched nutritive and disease-resistant growth-promoting; Mould also can secrete abundant enzyme, impels starch, cellulose etc. in raw material to be decomposed into monose, need, and self mycoprotein content is higher for growth of microorganism; Bacillus can cellulase-producing, degraded cellulose, and containing not easily lethal gemma in bacillus, enters the digestive system of animal, can suppress the growth of harmful bacteria in enteron aisle when feeding with viable bacteria state.
Rice residue and manioc waste mixture are after mixed strains solid fermented, and a large amount of mycoproteins that microbial reproduction produces add protein content, improve the feeding value of rice residue and manioc waste; Crude fibre simultaneously in manioc waste is converted into small-molecule substance through fermentable, improves the palatability of manioc waste as feed, is also easy to animal and absorbs; The various enzyme that produces of microorganism and active factors can suppress the growth of harmful bacteria in enteron aisle, promote the breeding of useful thalline in animal body in addition, thus improve immunity and the resistance against diseases of animal.
Below, the technical characteristic of the mixed strains solid fermented rice residue of the present invention and the method for manioc waste production high-quality high protein feed is further described in conjunction with the embodiments.
Detailed description of the invention
Embodiment 1: a kind of method of mixed strains solid fermented rice residue and manioc waste production high-quality high protein feed, comprises the following steps:
(1) preparation of liquid seeds: with nutrient broth Liquid Culture based on 37 DEG C cultivate bacillus subtilises (
bacillus subtilis) 24h, with potato dextrose medium (after potato being cleaned peeling stripping and slicing, take 200g and add water boil 20min, by eight layers of filtered through gauze, add 20g glucose in filtrate, then add water to 1000mL, use triangular flask packing, 121 DEG C of sterilizing 20min, cooling after for subsequent use) respectively at 28 DEG C cultivate aspergillus nigers (
aspergillus niger) 3-5d, in 28 DEG C cultivate brewer's yeasts (
brewer yeast) 2d, cultured bacterial classification is mixed and made into liquid seeds with the ratio of volume ratio Hei Qu Mei ︰ Pi wine Jiao Mu ︰ bacillus subtilis=2 ︰ 2 ︰ 1.
(2) rice residue (water content is lower than 10%) and dry manioc waste (water content is lower than 10%) equal proportion mixing (mass ratio is 1:1) will be done, add the water of the magnesium sulfate of mixture quality 0.2% and the dipotassium hydrogen phosphate of 0.4% and 30%, inoculate liquid seeds by 10% of dry rice residue and dry manioc waste mixture quality after mixing, at 28 DEG C-32 DEG C, cultivate 5-7d.
(3) pulverize after being dried in 40 ~ 60 DEG C by the material after fermentation, obtain the animal feeding-stuff containing somatic protein of rice residue and manioc waste mixed culture fermentation, its protein content is greater than 60%.
Claims (3)
1. a method for mixed strains solid fermented rice residue and manioc waste production high-quality high protein feed, is characterized in that: comprise the following steps:
(1) the preparation of liquid seeds: cultivate bacillus subtilis 22-26h based on 35-40 DEG C by nutrient broth Liquid Culture, cultivate aspergillus niger 3-5d with potato dextrose medium respectively at 25-30 DEG C, cultivate brewer's yeast 2-3d in 25-30 DEG C, cultured bacterial classification is mixed and made into liquid seeds with the ratio of volume ratio Hei Qu Mei ︰ Pi wine Jiao Mu ︰ bacillus subtilis=1.5-2.5 ︰ 1.5-2.5 ︰ 1;
(2) be obtain mixture after 0.5-2 ︰ 1 mixes in mass ratio by dry rice residue and dry manioc waste, then the magnesium sulfate of mixture quality 0.1-0.3%, the dipotassium hydrogen phosphate of 0.3-0.5% and the water of 25-35% is added, liquid seeds is inoculated, in 28 DEG C-32 DEG C bottom fermentation 5-7d by the 8-12% of dry rice residue and dry manioc waste mixture quality after mixing;
(3) pulverize after being dried in 40 ~ 60 DEG C by the material after fermentation, get product, described finished product is the high protein feed that protein quality content reaches 50 ~ 75%;
Described dry rice residue take rice as the accessory substance that starch sugar produced by raw material, protein content more than 60% in rice residue.
2. the method for mixed strains solid fermented rice residue according to claim 1 and manioc waste production high-quality high protein feed, is characterized in that: described dry rice residue and the water content of dry manioc waste are lower than 10%.
3. the method for mixed strains solid fermented rice residue according to claim 1 and 2 and manioc waste production high-quality high protein feed, it is characterized in that: the preparation method of described potato dextrose medium is: after potato being cleaned peeling stripping and slicing, take 200g and add water boil 20min, by eight layers of filtered through gauze, add 20g glucose in filtrate, then add water to 1000mL, use triangular flask packing, 121 DEG C of sterilizing 20min, for subsequent use after cooling.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103918874B (en) * | 2014-04-11 | 2015-10-21 | 四川农业大学 | A kind of method utilizing mixed fungus fermentation technology to improve manioc waste quality |
| CN104560783A (en) * | 2014-12-05 | 2015-04-29 | 广西大学 | Bacillus subtilis enrichment culture medium and application thereof |
| CN105265790B (en) * | 2015-11-17 | 2018-10-02 | 天津科技大学 | The cassava alcohol dreg fodder and the preparation method and application thereof of multiple-microorganism fermentation |
| CN106566857A (en) * | 2016-11-01 | 2017-04-19 | 武威传森生物技术有限公司 | Process for producing yeast protein from peel residues generated in whole potato flour production process |
| CN106509421A (en) * | 2016-12-21 | 2017-03-22 | 驻马店华中正大有限公司 | Microecological preparation culture medium for cattle prepared from guar meal and cassava residues |
| CN107047946A (en) * | 2017-01-05 | 2017-08-18 | 哈尔滨伟平科技开发有限公司 | A kind of preparation method of cassava residue fermented feed |
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| CN1502257A (en) * | 2002-11-21 | 2004-06-09 | 黄祥峰 | Method for producing protein fodder by rice residue fermentation |
| CN102934737A (en) * | 2012-12-10 | 2013-02-20 | 广西高源淀粉有限公司 | Preparation method of protein feed |
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| CN1502257A (en) * | 2002-11-21 | 2004-06-09 | 黄祥峰 | Method for producing protein fodder by rice residue fermentation |
| CN102934737A (en) * | 2012-12-10 | 2013-02-20 | 广西高源淀粉有限公司 | Preparation method of protein feed |
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