CN105146060A - Method for preparing animal feed by fermentation of rumen microorganisms - Google Patents
Method for preparing animal feed by fermentation of rumen microorganisms Download PDFInfo
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Abstract
The invention relates to a method for preparing an animal feed by the fermentation of rumen microorganisms and belongs to the field of feed preparation. The method comprises the following steps: by taking gingko leaves as a fermentation primer, adding nitrogen and phosphorus, glucose and the like as nutrient substances; inoculating rumen content; carrying out anaerobic fermentation and then inoculating saccharomycetes to continually carry out aerobic fermentation; drying a fermentation product to prepare a feed semi-finished product; then taking a wheat vinasse liquid in a rectification section and inoculating the saccharomycetes to continually ferment; and drying and mixing the product with the feed semi-finished product, crushing and sieving, and packaging to obtain the animal feed prepared by the fermentation of the rumen microorganisms. The feed prepared by the invention contains a lot of cellulose type nutrient substances and contains a lot of protein, so that the growth and development of animals are facilitated.
Description
Technical field
The present invention relates to the preparation method that a kind of rumen microbial fermentation prepares animal feed, belong to feed preparation field.
Background technology
Cud is specific to ruminant, and be a stomach maximum in the middle of adult ruminant four stomaches, account for 80% of four total stomach volumes, inside have a large amount of microbial growth, a big chunk of feed digests at this.Rumen microorganism can digest crude fibre, decomposes sugar, starch and protein; Synthesizing amino acid and protein.
Rumen content, be the ruminant domestic animal fodder grass of searching for food under the effect of rumen microorganism flora, the nutritious chyme that fermentation produces, it contains not digested careless feed, microorganism species, metabolite and gastric juice etc.Butcher the feed resource that the rumen content after cattle and sheep can be used as livestock and poultry utilization after suitably processing.
Rumen microorganism needs strict anaerobic environment and suitable temperature, once expose in atmosphere, anaerobic bacteria wherein, protozoon etc. then can be very fast dead; Therefore, if can keep the microorganism species in rumen content, introduce laboratory and carry out anaerobic fermentation, can be used as the bacterium source of fermentation, digestion degraded cellulose, the Substance Transformation being difficult to by animal digest, absorb is the material easily digested and assimilated.
Starch-containing in the composition of wheat, the composition such as protein and fat, nutrition is very abundant, is worth very high.China's wheat yield and all very high only Wheat in Henan Province output of deposit are just at 2150 ~ 2,350 ten thousand tons.But because market continues weak, raw grain is sold and is restricted, and wheat reserves have surpassed the expectation 1,200 ten thousand tons.The continuing to increase of alcohol demand in recent years thereupon, the deep processing utilizing Wheat Production alcohol to can be wheat provides channel.Little brewer's grains is exactly the accessory substance producing alcohol.
Be rich in the nutriments such as abundant organic matter, Yeast protein, carbohydrate in little brewer's grains, and vinasse viscosity is difficult to more greatly be separated, process bad meeting and cause the wasting of resources and environmental pollution, the pollution that present fermentation industry brings is only second to paper-making industry.The report of the domestic and international process for little brewer's grains is also relatively less now, and vinasse process relies on the chemical method in later stage substantially, and not only cost is high, also result in certain wasting of resources; More seriously all can not process up to standard to the later stage, therefore how can reduce the waste of resource, can effectively utilize again this resource significant.
Summary of the invention
The technical problem that the present invention mainly solves: for the enzyme not containing energy digest cellulose in current most poultry livestock body, utilize insufficient to the food nutrition of cellulose family, and animal feed protein content grade on the low side is not enough, provide a kind of method that rumen microbial fermentation prepares animal feed, the method take ginkgo leaf as fermentation substrate, nutriment is made by adding nitrogen phosphorus and glucose etc., inoculation rumen content, first anaerobic fermentation, inoculation yeast bacterium continues aerobic fermentation afterwards, the obtained feed semi-finished product of tunning drying, the wheat wine lees liquor inoculation yeast bacterium of getting rectification section afterwards again continues fermentation, pulverize and sieve after mixing with feed semi-finished product after dry, namely animal feed prepared by a kind of rumen microbial fermentation is obtained after packaging.The present invention obtains feed and comprises a large amount of cellulose family nutriment, and protein content is very high, is conducive to animal growth.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) 300 ~ 400g ginkgo leaf is got, with deionized water rinsing 3 ~ 4 times, clean surperficial floating dust, 4 ~ 5h is tanned by the sun afterwards under burning sun, ginkgo leaf after drying is put into airslide disintegrating mill pulverize, put into 1L beaker, add 10 ~ 15g potassium dihydrogen phosphate, 8 ~ 10g ammonium sulfate, 15 ~ 20g magnesium sulfate and 12 ~ 15g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, 30 ~ 40min is stirred, obtained ginkgo leaf fermented and cultured mixture with the rotating speed of 300 ~ 400r/min;
(2) in the cud the fresh ox stomach of just having butchered, rumen content is taken out, taking 100 ~ 200g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 2 ~ 3 days at 35 ~ 45 DEG C;
(3) film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 4 ~ 5 days under the condition of 25 ~ 35 DEG C, afterwards tunning is moved into concentration tank, reduce pressure to 1100 ~ 1200Pa in tank and carry out reduced pressure concentration, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 3 ~ 5h under the condition of 105 ~ 110 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product;
(4) the wheat wine lees liquor of 1 ~ 2L rectification section is got, put into rotary evaporator, heating evaporation carries out concentration 1 ~ 2h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 50 ~ 100g wheat bran, 15 ~ 30g yeast extract, 15 ~ 20g peptone, 30 ~ 40g corn flour and 30 ~ 40g glucose and add 500 ~ 1000mL distilled water, inoculation yeast bacterium, sealing and fermenting 10 ~ 15h after stirring by stirring rod, fermentation temperature is 35 ~ 45 DEG C;
(5) fermentate is poured into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 30 ~ 40 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
Described normal pressure pressure pressure is 0.5 ~ 0.8MPa.
Embody rule method of the present invention: get the animal feed that the present invention obtains and feed young pig, within one day, feed 3 ~ 4 times, each dosage is 500 ~ 1000g, with the young pig of this forage feed compared with the young pig of other forage feeds same period, body weight exceeds more than 30%, has clear superiority.
The invention has the beneficial effects as follows: the animal feed that the present invention obtains is rich in cellulose family nutriment, improve food nutrition utilization rate, protein content is high, is conducive to animal growth.
Detailed description of the invention
Get 300 ~ 400g ginkgo leaf, with deionized water rinsing 3 ~ 4 times, clean surperficial floating dust, under burning sun, tan by the sun 4 ~ 5h afterwards, the ginkgo leaf after drying is put into airslide disintegrating mill and pulverizes, put into 1L beaker, add 10 ~ 15g potassium dihydrogen phosphate, 8 ~ 10g ammonium sulfate, 15 ~ 20g magnesium sulfate and 12 ~ 15g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, stir 30 ~ 40min with the rotating speed of 300 ~ 400r/min, obtained ginkgo leaf fermented and cultured mixture; Rumen content is taken out in the cud the fresh ox stomach of just having butchered, taking 100 ~ 200g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 2 ~ 3 days at 35 ~ 45 DEG C; Film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 4 ~ 5 days under the condition of 25 ~ 35 DEG C, afterwards tunning is moved into concentration tank, reduce pressure to 1100 ~ 1200Pa in tank and carry out reduced pressure concentration, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 3 ~ 5h under the condition of 105 ~ 110 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product; Get the wheat wine lees liquor of 1 ~ 2L rectification section, put into rotary evaporator, heating evaporation carries out concentration 1 ~ 2h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 50 ~ 100g wheat bran, 15 ~ 30g yeast extract, 15 ~ 20g peptone, 30 ~ 40g corn flour and 30 ~ 40g glucose and add 500 ~ 1000mL distilled water, inoculation yeast bacterium, sealing and fermenting 10 ~ 15h after stirring by stirring rod, fermentation temperature is 35 ~ 45 DEG C; Pour fermentate into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 30 ~ 40 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
Described normal pressure pressure pressure is 0.5 ~ 0.8MPa.
Example 1
Get 300g ginkgo leaf, with deionized water rinsing 3 times, clean surperficial floating dust, under burning sun, tan by the sun 4h afterwards, the ginkgo leaf after drying is put into airslide disintegrating mill and pulverizes, put into 1L beaker, add 10g potassium dihydrogen phosphate, 8g ammonium sulfate, 15g magnesium sulfate and 12g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, stir 30min with the rotating speed of 300r/min, obtained ginkgo leaf fermented and cultured mixture; Rumen content is taken out in the cud the fresh ox stomach of just having butchered, taking 100g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 2 days at 35 DEG C; Film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 4 days under the condition of 25 DEG C, afterwards tunning is moved into concentration tank, reduce pressure in tank and carry out reduced pressure concentration to 1100Pa, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 3h under the condition of 105 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product; Get the wheat wine lees liquor of 1L rectification section, put into rotary evaporator, heating evaporation carries out concentration 1h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 50g wheat bran, 15g yeast extract, 15g peptone, 30g corn flour and 30g glucose and add 500mL distilled water, inoculation yeast bacterium, sealing and fermenting 10h after stirring by stirring rod, fermentation temperature is 35 DEG C; Pour fermentate into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 30 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
Described normal pressure pressure pressure is 0.5MPa.
Embody rule method of the present invention: get the animal feed that the present invention obtains and feed young pig, within one day, feed 3 times, each dosage is 500g, and with the young pig of this forage feed compared with the young pig of other forage feeds same period, body weight exceeds 32%, has clear superiority.
Example 2
Get 350g ginkgo leaf, with deionized water rinsing 3 times, clean surperficial floating dust, under burning sun, tan by the sun 4h afterwards, the ginkgo leaf after drying is put into airslide disintegrating mill and pulverizes, put into 1L beaker, add 13g potassium dihydrogen phosphate, 9g ammonium sulfate, 18g magnesium sulfate and 14g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, stir 35min with the rotating speed of 350r/min, obtained ginkgo leaf fermented and cultured mixture; Rumen content is taken out in the cud the fresh ox stomach of just having butchered, taking 150g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 2 days at 40 DEG C; Film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 4 days under the condition of 30 DEG C, afterwards tunning is moved into concentration tank, reduce pressure in tank and carry out reduced pressure concentration to 1150Pa, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 4h under the condition of 108 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product; Get the wheat wine lees liquor of 1L rectification section, put into rotary evaporator, heating evaporation carries out concentration 1h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 80g wheat bran, 20g yeast extract, 18g peptone, 35g corn flour and 35g glucose and add 800mL distilled water, inoculation yeast bacterium, sealing and fermenting 13h after stirring by stirring rod, fermentation temperature is 40 DEG C; Pour fermentate into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 35 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
Described normal pressure pressure pressure is 0.7MPa.
Embody rule method of the present invention: get the animal feed that the present invention obtains and feed young pig, within one day, feed 3 times, each dosage is 800g, and with the young pig of this forage feed compared with the young pig of other forage feeds same period, body weight exceeds 35%, has clear superiority.
Example 3
Get 400g ginkgo leaf, with deionized water rinsing 4 times, clean surperficial floating dust, under burning sun, tan by the sun 5h afterwards, the ginkgo leaf after drying is put into airslide disintegrating mill and pulverizes, put into 1L beaker, add 15g potassium dihydrogen phosphate, 10g ammonium sulfate, 20g magnesium sulfate and 15g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, stir 40min with the rotating speed of 400r/min, obtained ginkgo leaf fermented and cultured mixture; Rumen content is taken out in the cud the fresh ox stomach of just having butchered, taking 200g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 3 days at 45 DEG C; Film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 5 days under the condition of 35 DEG C, afterwards tunning is moved into concentration tank, reduce pressure in tank and carry out reduced pressure concentration to 1200Pa, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 5h under the condition of 110 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product; Get the wheat wine lees liquor of 2L rectification section, put into rotary evaporator, heating evaporation carries out concentration 2h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 100g wheat bran, 30g yeast extract, 20g peptone, 40g corn flour and 40g glucose and add 1000mL distilled water, inoculation yeast bacterium, sealing and fermenting 15h after stirring by stirring rod, fermentation temperature is 45 DEG C; Pour fermentate into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 40 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
Described normal pressure pressure pressure is 0.8MPa.
Embody rule method of the present invention: get the animal feed that the present invention obtains and feed young pig, within one day, feed 4 times, each dosage is 1000g, and with the young pig of this forage feed compared with the young pig of other forage feeds same period, body weight exceeds 38%, has clear superiority.
Claims (2)
1. rumen microbial fermentation prepares a method for animal feed, it is characterized in that concrete preparation process is:
(1) 300 ~ 400g ginkgo leaf is got, with deionized water rinsing 3 ~ 4 times, clean surperficial floating dust, 4 ~ 5h is tanned by the sun afterwards under burning sun, ginkgo leaf airslide disintegrating mill after drying is pulverized, put into 1L beaker, add 10 ~ 15g potassium dihydrogen phosphate, 8 ~ 10g ammonium sulfate, 15 ~ 20g magnesium sulfate and 12 ~ 15g phosphoamide, be that 1:3 adds distilled water and is placed on magnetic stirrer by solid-to-liquid ratio, 30 ~ 40min is stirred, obtained ginkgo leaf fermented and cultured mixture with the rotating speed of 300 ~ 400r/min;
(2) in the cud the fresh ox stomach of just having butchered, rumen content is taken out, taking 100 ~ 200g rumen content puts in fermentation tank, add above-mentioned obtained ginkgo leaf fermented and cultured mixture, backward fermentation tank in pass into nitrogen and drain air, seal tank mouth with film, put into incubator anaerobic fermentation 2 ~ 3 days at 35 ~ 45 DEG C;
(3) film is opened after anaerobic fermentation, saccharomycete is inoculated into fermentation tank, aerobic fermentation 4 ~ 5 days under the condition of 25 ~ 35 DEG C, afterwards tunning is moved into concentration tank, reduce pressure to 1100 ~ 1200Pa in tank and carry out reduced pressure concentration, obtain pureed concentrate, again concentrate is put into thermostatic drying chamber, dry 3 ~ 5h under the condition of 105 ~ 110 DEG C, puts into pulverizer and pulverizes by desciccate, cross 30 mesh standard sieves and obtain feed semi-finished product;
(4) the wheat wine lees liquor of 1 ~ 2L rectification section is got, put into rotary evaporator, heating evaporation carries out concentration 1 ~ 2h to it, obtain little brewer's grains concentrate, be poured into fermentation tank, add 50 ~ 100g wheat bran, 15 ~ 30g yeast extract, 15 ~ 20g peptone, 30 ~ 40g corn flour and 30 ~ 40g glucose and add 500 ~ 1000mL distilled water, inoculation yeast bacterium, sealing and fermenting 10 ~ 15h after stirring by stirring rod, fermentation temperature is 35 ~ 45 DEG C;
(5) fermentate is poured into chamber-type press filter, just suppress dehydration, discharge zymotic fluid afterwards, obtain vinasse fermentation filter cake, filter cake put into vacuum desiccator and carry out drying, after dry with above-mentioned obtained feed semi-finished product in mass ratio for 1:2 mixes, put into airslide disintegrating mill afterwards to pulverize, and cross 30 ~ 40 mesh standard sieves, obtain granulated meal finished product, after packaging, namely obtain animal feed prepared by a kind of rumen microbial fermentation.
2. a kind of rumen microbial fermentation according to claim 1 prepares the preparation method of animal feed, it is characterized in that: described normal pressure pressure pressure is 0.5 ~ 0.8MPa.
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Cited By (6)
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| CN105533138A (en) * | 2015-12-31 | 2016-05-04 | 丁建红 | Ginkgo biloba life benefiting and livestock fattening additive |
| CN105901304A (en) * | 2016-04-18 | 2016-08-31 | 广州市佰沃生物科技有限公司 | Novel animal feedstuff and a preparing method thereof |
| CN106333060A (en) * | 2016-08-26 | 2017-01-18 | 曹蕊 | Microbial fermentation feed and preparation method and application thereof |
| CN106720968A (en) * | 2016-12-08 | 2017-05-31 | 李桉 | Rumen content as feed processing method |
| CN107535699A (en) * | 2017-09-04 | 2018-01-05 | 安徽五粮泰生物工程股份有限公司 | A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe |
| CN111670861A (en) * | 2020-06-17 | 2020-09-18 | 西藏自治区农牧科学院畜牧兽医研究所 | Production and application of rumen fluid for fattening sheep |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533138A (en) * | 2015-12-31 | 2016-05-04 | 丁建红 | Ginkgo biloba life benefiting and livestock fattening additive |
| CN105901304A (en) * | 2016-04-18 | 2016-08-31 | 广州市佰沃生物科技有限公司 | Novel animal feedstuff and a preparing method thereof |
| CN106333060A (en) * | 2016-08-26 | 2017-01-18 | 曹蕊 | Microbial fermentation feed and preparation method and application thereof |
| CN106720968A (en) * | 2016-12-08 | 2017-05-31 | 李桉 | Rumen content as feed processing method |
| CN107535699A (en) * | 2017-09-04 | 2018-01-05 | 安徽五粮泰生物工程股份有限公司 | A kind of preparation method rich in volatile fatty acid calcium cud source fermentative feedstuff of microbe |
| CN111670861A (en) * | 2020-06-17 | 2020-09-18 | 西藏自治区农牧科学院畜牧兽医研究所 | Production and application of rumen fluid for fattening sheep |
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