CN1623415A - Multiple composite microbiological protein feed - Google Patents

Multiple composite microbiological protein feed Download PDF

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Publication number
CN1623415A
CN1623415A CNA2003101115201A CN200310111520A CN1623415A CN 1623415 A CN1623415 A CN 1623415A CN A2003101115201 A CNA2003101115201 A CN A2003101115201A CN 200310111520 A CN200310111520 A CN 200310111520A CN 1623415 A CN1623415 A CN 1623415A
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carrier
multiple composite
protein feed
edible
microbial protein
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CN100364439C (en
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胡全义
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

A composite microbial protein feed is prepared through preparing the composite useful microbe colony from rumen microbe colony and its carrier, edible fungus colony and its carrier and effective microbe colony, using it in biologic fermentation of cheap rejected resource, and enzyme-catalytic processing. Its advantages are high nutritive value, easy absorption, low cost, and no pollution.

Description

A kind of MULTIPLE COMPOSITE microbial protein feed
Technical field:
The invention belongs to the using microbe engineering and carry out the technical field of livestock and poultry, Aquatic Feed Processing, what relate to specifically is a kind of MULTIPLE COMPOSITE microbial protein feed.
Background technology:
Utilize biofermentation and enzyme catalytic action modern age, conventional feed was handled, can improve the livestock and poultry digestibility, reduced disease, improved breeding environment, reduced feeding cost, produced non-harmful meat, egg, milk deli.Appear on the market with leavening, additive but biological products are many; its beneficial microbe population polymerization amount is not high; disposal ability to waste resource also is subjected to certain limitation; and the user needs preparation separately, fermentation can be fed once more; the outstanding sense inconvenience of large-scale cultivation industry; be difficult to popularize, cause the animal husbandry integral level to promote slowly the growth requirement of the incompatibility ecological agriculture.
Summary of the invention:
In order to solve the deficiencies in the prior art part, the invention provides a kind of microorganism polymerization amount height,, the MULTIPLE COMPOSITE microbial protein feed that make things convenient for aquaculture directly feed strong to the waste resource disposal ability.
For achieving the goal, the invention provides following technical solution:
A kind of MULTIPLE COMPOSITE microbial protein feed, its quality prescription is:
Rumen microorganism group and carrier 5-50%
Edible flora and carrier 10-50%
Microorganism cluster nutrition carrier 30-80%
Nitrogenous source 4-8%
Molasses 0.4-1.5%
CEM (EM) 0.4-1.5%
Whole air-dry material meters 100% need to add an amount of moisture content in addition and make its total moisture content 30-42%.
Wherein:
The available known stomach catheter method of rumen microorganism group is gathered after two-stage expands numerous subculture kind, or with the healthy cattle and sheep rumen content of butchering, drying and crushing under 40-45 ℃ of condition is as rumen microorganism group provenance and carrier.
Edible flora can be with the edible fungus culturing Bag Material of sending out mycelia full, or with the useless Bag Material of adopting mushroom 2-4 tide, dried and crushed is as edible flora provenance and carrier.
1., vinasse residue of soya sugar slag, process hides waste oil, inferior assorted cake class industrial by-products microorganism cluster nutrition carrier can adopt:; 2., straw tendril pod shell, nontoxic low toxicity leaf weeds, inferior fruit vegetables plantation discarded object; 3., land useless feed, feces of livestock and poultry, butcher livestock and poultry blanking (gastric content, blood, hair, inferior meat bone) class and culture discarded object; 4., inferior fishes and shrimps, fishes and shrimps blanking, algae waste of aquatic; 5., Residual oil swill, rotten grain, oil, really, dish class domestic refuse; 6., low value coal dust, the cheap organic matter of peat class.These microorganism cluster nutrition carriers must carry out draining, making beating, drying, pulverization process respectively.Its aim is that local resources is abundant, quality is stable or the approximate substitution raw material is arranged, and cheapness is easy to gather nearby, after the preferred collocation, can receive and integrate easy, with low cost, environment purification, pollution abatement, balanced in nutrition, the profit of having complementary advantages.
The said MULTIPLE COMPOSITE microbial protein feed of the present invention, its production technology is:
(1) takes by weighing molasses by formula ratio and dissolve in the 35-40 ℃ of an amount of warm water, stir and make it off-bottom;
(2) taking by weighing CEM (EM liquid bacterium liquid such as the former dew of EM) by formula ratio adds in (1) and stirs evenly;
(3) getting the moisture content that drop goes out in an amount of microorganism cluster nutrition carrier processing procedure adds in (2);
(4) take by weighing nitrogenous source (urea, ammonium sulfate) by formula ratio and add in (3) and stir evenly, make it the fused composite bacteria liquid water that becomes;
(5) rumen microorganism group and carrier, edible flora and carrier, microorganism cluster nutrition carrier are carried out the cooperation of weighing after draining, making beating, drying, the pulverization process respectively, stir;
(6) (4) are added in (5), fully mix once more;
(7) (6) are packed in batches round successively covers completely with film after the compacting and guarantees sealing;
(8) finished through normal temperature laboratory 5-10 days and leave standstill anaerobic fermentation, become MULTIPLE COMPOSITE microbial protein feed product.
For the present invention, its product should detect crude protein, crude fat, crude fibre, coarse ash, physical and chemical indexs such as NFE, calcium, phosphorus, moisture content, pH value, other nutrition is according to condition, under raw materials enjoy stable sources, constant, the consistent prerequisite of technology of prescription, general chemical examination once gets final product, so that reduce error in displacement substitutes or prepares, ensures the quality of products and result of use.Qualified matured product is pale brown look, has slightly sour delicate fragrance vinosity to overflow, pH value≤6, and living bacteria count 〉=100,000,000/g, thick protein improves and reaches 28-36% more than 4 times, crude fibre degradation rate 40-80%.
For the present invention, the MULTIPLE COMPOSITE microbial protein feed took out from round airing 2-3 hour, both can directly pack as finished product, substituted adapted for aquaculture; Also can be at different breeding object nutritional need, it is directly feeding for aquaculture to add corn, mineral matter and other nutrient scale preparation Comprehesive biological feed finished product; Temporarily need not still can continue sealing deposits in the round.Connect the advantage of taking because huge MULTIPLE COMPOSITE beneficial microbe cluster has symbiosis propagation colony, as long as keep sealing anaerobism normal temperature environment, the storage period of guaranteeing the quality, all can be above six months.
The present invention compares with prior art: 1, integrate and become MULTIPLE COMPOSITE beneficial microbe cluster with rumen microorganism group, edible flora, CEM (EM) assembling, form the huge population advantage of the compatible propagation of symbiosis again, biologically active is stronger more lasting.2, owing to the efficient transformation of MULTIPLE COMPOSITE beneficial microbe cluster to potential nutrients such as crude fibres, can utilize the cheap waste resource of multiclass by vast scale, as farming accessory substances such as straw, leaves, domestic refuses such as industrial by-products such as vinasse, residue of soya and fishes and shrimps blanking, Residual oil swill, with low cost, the purifying ecological of turning waste into wealth serves multiple.3, can directly substitute conventional puffed soybean protein raw materials as finished product, and be convenient to prepare Comprehesive biological feed, all or partly replace traditional complete diet pellet, it is directly feeding to be beneficial to the large-scale cultivation industry especially.4, at fermentation vat, single finished product packing or in the sealing of the normal temperature laboratory after being mixed with the Comprehesive biological feed product anaerobic environment, MULTIPLE COMPOSITE beneficial microbe cluster is in vigorous breeding growing trend all the time, help prolonging the resting period of guaranteeing the quality, and optimize and improve the quality of products.5, the colony of MULTIPLE COMPOSITE beneficial microbe cluster even takes effect, and in biofermentation enzyme conversion process, secretion multiple nutrients material and somatomedin can be cleared up the murder by poisoning composition, significantly improve feed nutritive value comprehensively.6, product is fed can strengthen behind the livestock and poultry and is absorbed transformation efficiency, reduce disease and save the expenses for medicine degrade residual, improve to plant and strengthen breeding prepotency with the livestock and poultry breeding potential, eliminate hurdle house stench and implement ecologic breeding, reduce feeding cost and increase effective output, the healthy free of contamination high-quality green animal derived food of production safety has ecology, economical, societal benefits concurrently, is beneficial to sustainable development.
Description of drawings
Accompanying drawing is the MULTIPLE COMPOSITE microbial protein feed technological process of production
Specific embodiments:
Embodiment one:
The rumen microorganism group gathers after two-stage expands numerous subculture kind with known stomach catheter method, and drying and crushing under 40 ℃ of conditions is as rumen microorganism group provenance and carrier.
Edible flora is with adopting the useless Bag Material that mushroom 2-4 criticizes, and dried and crushed is as edible flora provenance and carrier.
1., industrial by-products brewex's grains, Chinese sorghum, maize alcohol stillage microorganism cluster nutrition carrier adopts:; 2., plantation discarded object broad bean cauline leaf pod shell, Folium sophorae, green hay; 3., waste of aquatic fishes and shrimps blanking; 4., domestic refuse Residual oil swill.
Wherein, the quality prescription is:
1, rumen microorganism group and carrier (40 ℃ of dry down, pulverizing) 5KG
2, edible flora and carrier, (nothing is gone mouldy, and dries the back and pulverizes) 10KG
3, microorganism cluster nutrition carrier (air-dry material 79.5KG), wherein
3.1, broad bean cauline leaf pod shell, Folium sophorae, green hay (dry, pulverize) 32KG
3.2, brewex's grains, Chinese sorghum, maize alcohol stillage (dry, pulverize) 30KG
3.3, fishes and shrimps blanking, Residual oil swill (draining to water content 65%, making beating) 50KG
4, nitrogenous source (urea, ammonium sulfate half and half) 4KG
5, molasses (brown sugar) 1.5KG
6, CEM (EM) 1.5KG
Add up to 134KG, wherein air-dry material 100KG.
The MULTIPLE COMPOSITE microbial protein feed, its production technology is:
(1) takes by weighing molasses by formula ratio and dissolve in 40 ℃, 3KG warm water, stir and make it off-bottom;
(2) taking by weighing CEM (EM liquid bacterium liquid such as the former dew of EM) by formula ratio adds in (1) and stirs evenly;
(3) get the band grease moisture content 8KG that drop goes out in the prescription 3.3 and add mixing in (2);
(4) take by weighing nitrogenous source (urea, ammonium sulfate) by formula ratio and add in (3) and stir evenly, make it the fused composite bacteria liquid water that becomes;
(5) rumen microorganism group and carrier, edible flora and carrier, microorganism cluster nutrition carrier are carried out the cooperation of weighing after draining, making beating, drying, the pulverization process respectively, stir;
(6) (4) are added in (5), fully mix once more;
(7) (6) are packed in batches round (cylinder) successively covers completely with film after the compacting and guarantees sealing;
(8) through 25 ℃ of normal temperature laboratories, finished in 5 days and leave standstill anaerobic fermentation, become MULTIPLE COMPOSITE microbial protein feed product.
Embodiment two:
The rumen microorganism group uses the healthy cattle and sheep rumen content of butchering, and drying and crushing under 40 ℃ of conditions is as rumen microorganism group provenance and carrier.
Edible flora is with the edible fungus culturing Bag Material of sending out mycelia full, and dried and crushed is as edible flora provenance and carrier.
1., industrial by-products alcohol end, residue of soya, maltose residues microorganism cluster nutrition carrier adopts:; 2., plantation discarded object peanut seedling, reed leaf; 3., culture discarded object laying hen excrement; 4., the inferior fishes and shrimps of waste of aquatic; 5., domestic refuse Residual oil swill; 6., cheap organic matter low value coal dust.
Wherein, the quality prescription is:
1, rumen microorganism group and carrier (40 ℃ of dry down, pulverizing) 15KG
2, edible flora and carrier, (drying the back pulverizes) 20KG
3, microorganism cluster nutrition carrier (air-dry material 56.5KG), wherein
3.1, peanut seedling, laying hen excrement, reed leaf (dry, pulverize) 11KG
3.2, alcohol end, residue of soya, maltose residues (dry, pulverize) 26KG
3.3, inferior fishes and shrimps, Residual oil swill (draining to water content 65%, making beating) 51.6KG
3.4, low value coal dust 1.6KG
4, nitrogenous source (urea, ammonium sulfate half and half) 8KG
5, molasses (yellow sand sugar) 0.4KG
6, CEM (EM) 0.4KG
Add up to 134KG, wherein air-dry material 100KG.
The MULTIPLE COMPOSITE microbial protein feed, its production technology is:
(1) takes by weighing molasses by formula ratio and dissolve in 40 ℃, 3KG warm water, stir and make it off-bottom;
(2) taking by weighing CEM (EM liquid bacterium liquid such as the former dew of EM) by formula ratio adds in (1) and stirs evenly;
(3) get the band grease moisture content 8KG that drop goes out in the prescription 3.3 and add mixing in (2);
(4) take by weighing nitrogenous source (urea, ammonium sulfate) by formula ratio and add in (3) and stir evenly, make it the fused composite bacteria liquid water that becomes;
(5) rumen microorganism group and carrier, edible flora and carrier, microorganism cluster nutrition carrier are carried out the cooperation of weighing after draining, making beating, drying, the pulverization process respectively, stir;
(6) (4) are added in (5), fully mix once more;
(7) (6) are packed in batches round successively covers completely with film after the compacting and guarantees sealing;
(8) through 15 ℃ of normal temperature laboratories, finished in 10 days and leave standstill anaerobic fermentation, become MULTIPLE COMPOSITE microbial protein feed product.

Claims (6)

1, a kind of MULTIPLE COMPOSITE microbial protein feed, it is characterized in that: be integrated into MULTIPLE COMPOSITE beneficial microbe cluster with rumen microorganism group and carrier, edible flora and carrier, CEM (EM) assembling, the cheap waste resource of multiclass is carried out the catalysis of biofermentation enzyme, make after the processing.
2, MULTIPLE COMPOSITE microbial protein feed according to claim 1 is characterized in that: its quality prescription is:
Rumen microorganism group and carrier 5-50%
Edible flora and carrier 10-50%
Microorganism cluster nutrition carrier 30-80%
Nitrogenous source 4-8%
Molasses 0.4-1.5%
CEM (EM) 0.4-1.5%
Whole air-dry material meters 100% need to add an amount of moisture content in addition and make its total moisture content 30-42%.
3, MULTIPLE COMPOSITE microbial protein feed according to claim 1 and 2, it is characterized in that: the available known stomach catheter method of described rumen microorganism group is gathered after two-stage expands numerous subculture kind, or with the healthy cattle and sheep rumen content of butchering, drying and crushing under 40-45 ℃ of condition is as rumen microorganism group provenance and carrier.
4, MULTIPLE COMPOSITE microbial protein feed according to claim 1 and 2 is characterized in that: described edible flora can be with the edible fungus culturing Bag Material of sending out mycelia full, or with the useless Bag Material of adopting mushroom 2-4 tide, dried and crushed is as edible flora provenance and carrier.
1., vinasse residue of soya sugar slag, process hides waste oil, inferior assorted cake class industrial by-products 5, MULTIPLE COMPOSITE microbial protein feed according to claim 2 is characterized in that: described microorganism cluster nutrition carrier can adopt:; 2., straw tendril pod shell, nontoxic low toxicity leaf weeds, inferior fruit vegetables plantation discarded object; 3., land useless feed, feces of livestock and poultry, butcher livestock and poultry blanking (gastric content, blood, hair, inferior meat bone) class and culture discarded object; 4., inferior fishes and shrimps, fishes and shrimps blanking, algae waste of aquatic; 5., Residual oil swill, rotten grain, oil, really, dish class domestic refuse; 6., low value coal dust, the cheap organic matter of peat class.
6, the said MULTIPLE COMPOSITE microbial protein feed of the present invention, its production technology is:
(1) takes by weighing molasses by formula ratio and dissolve in the 35-40 ℃ of an amount of warm water, stir and make it off-bottom;
(2) taking by weighing CEM (EM liquid bacterium liquid such as the former dew of EM) by formula ratio adds in (1) and stirs evenly;
(3) get the moisture content that drop goes out in an amount of microorganism cluster nutrition carrier processing procedure and add mixing in (2);
(4) take by weighing nitrogenous source (urea, ammonium sulfate) by formula ratio and add in (3) and stir evenly, make it the fused composite bacteria liquid water that becomes;
(5) with draining, making beating, drying, the pulverizing cooperation of weighing respectively of rumen microorganism group and carrier, edible flora and carrier, microorganism cluster nutrition carrier, stir;
(6) (4) are added in (5), fully mix once more;
(7) (6) are packed in batches round successively covers completely with film after the compacting and guarantees sealing;
(8) finished through normal temperature laboratory 5-10 days and leave standstill anaerobic fermentation, become MULTIPLE COMPOSITE microbial protein feed product.
CNB2003101115201A 2003-12-04 2003-12-04 Multiple composite microbiological protein feed Expired - Fee Related CN100364439C (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101074420B (en) * 2006-05-18 2011-04-06 上海创博生态工程有限公司 Polypeptide and microbial preparation for improving ox myotendinous development and its production
CN105146060A (en) * 2015-10-19 2015-12-16 高大元 Method for preparing animal feed by fermentation of rumen microorganisms
CN105410360A (en) * 2015-11-27 2016-03-23 全椒县马厂镇周巷山羊养殖场 Mutton meat improving fermented feed and preparation method thereof
CN105581038A (en) * 2016-01-23 2016-05-18 李聚才 Corncob feed prepared from urea, ammonium sulfate and molasses through composite processing and preparation method of corncob feed
CN105767508A (en) * 2016-03-15 2016-07-20 天津生态城环保有限公司 Protein feed production method by mixing kitchen waste and vinegar residue
CN107439796A (en) * 2017-08-11 2017-12-08 广东省生物工程研究所(广州甘蔗糖业研究所) A kind of preparation method of high energy high protein feed

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1061891A (en) * 1990-12-08 1992-06-17 四川省自然资源研究所 Make the method and the special equipment of rumen feed
JPH08205785A (en) * 1995-02-03 1996-08-13 Ii M Kenkyu Kiko:Kk Phytin-decomposing feed
CN1223081A (en) * 1998-09-23 1999-07-21 李维炯 Biological active fodder additives
JP3942938B2 (en) * 2002-04-11 2007-07-11 株式会社Em研究機構 Daphnia culture method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101074420B (en) * 2006-05-18 2011-04-06 上海创博生态工程有限公司 Polypeptide and microbial preparation for improving ox myotendinous development and its production
CN105146060A (en) * 2015-10-19 2015-12-16 高大元 Method for preparing animal feed by fermentation of rumen microorganisms
CN105410360A (en) * 2015-11-27 2016-03-23 全椒县马厂镇周巷山羊养殖场 Mutton meat improving fermented feed and preparation method thereof
CN105581038A (en) * 2016-01-23 2016-05-18 李聚才 Corncob feed prepared from urea, ammonium sulfate and molasses through composite processing and preparation method of corncob feed
CN105767508A (en) * 2016-03-15 2016-07-20 天津生态城环保有限公司 Protein feed production method by mixing kitchen waste and vinegar residue
CN107439796A (en) * 2017-08-11 2017-12-08 广东省生物工程研究所(广州甘蔗糖业研究所) A kind of preparation method of high energy high protein feed

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Assignee: Shandong Licun Biotechnology Co., Ltd.

Assignor: Hu Quanyi

Contract fulfillment period: 2009.6.1 to 2015.5.31 contract change

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Denomination of invention: Multiple composite microbiological protein feed

Granted publication date: 20080130

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LIC Patent licence contract for exploitation submitted for record

Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.6.1 TO 2015.5.31; CHANGE OF CONTRACT

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