JPH08205785A - Phytin-decomposing feed - Google Patents

Abstract

PURPOSE: To obtain the subject feed whose phytin is easily absorbed and utilized in a form of inositol in a body of an animal, capable of reducing phosphorus in excrementitious matter, and accordingly useful for improving environmental pollution by compounding a phytin-containing grain with a specific microorganism and a phytase. CONSTITUTION: This feed is produced by compounding a phytin-containing grain (preferably, rice bran) with an effective microbial group (EM) having an acidophilic property, producing lactic acid, without producing butyric acid, free from pathogenicity and at the same time having an antagonistic value of >=50, and a phytase. Examples of the EM include e.g. Streptomyces sp. (ATCC 3004) belonging to the group of actinomyces and Lactobacillus sp. (IFO 3070) belonging to the group of lactobaci1li. Further, a preferable amount of addition of the EM is 0.2wt.% in terms of bacterial body weight based on a grain material. Furthermore, the amount of the addition of the phytase is preferably 1-2wt.% based on the phytin in the grain material.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a phytinolytic feed used in the field of livestock feed.

[0002]

2. Description of the Related Art Generally, grains such as rice bran and corn contain a relatively large amount of phytin. As is well known, the phytin has an extremely effective physiologically active action such as a growth promoting action in animals. It is being researched as a feed additive because it decomposes and converts into inositol.

Particularly in recent years, countermeasures for effectively utilizing phytin have been studied mainly in the advanced livestock countries of Europe and the United States. For example, an enzyme such as phytase is added to grain feed containing phytin to improve the body of animals. Therefore, attempts have been made to decompose and convert phytin into inositol, and to easily absorb the phosphorus in phytin into the body to save the phosphorus component added to the compound feed.

On the other hand, with regard to inositol used as an additive for feeds, the present condition is that the inositol extracted and manufactured by a chemical method using defatted rice bran etc. as a raw material is added to the rice bran etc. again.

[0005]

The conventional grain feed usually contains about 10% of phytin, but phytin is not easily decomposed in the body of an animal and is not effectively absorbed as inositol, so it is excreted as manure. As a result, it causes environmental pollution due to phosphorus enrichment on the river coast. In addition, the method of re-adding inositol extracted and produced by a chemical method has a large cost loss.

[0006] An object and object of the present invention is to provide a cereal feed capable of easily absorbing and utilizing phytin as inositol in the body of an animal, and further to provide a phytin-degrading feed which is useful for improving environmental pollution related to animal husbandry. To provide.

[0007]

Means for Solving the Problems The phytin-degrading feed of the present invention for solving the above-mentioned problems does not have pathogenicity in cereals containing phytin because it does not produce lactic acid and butyric acid with anti-acidity. And effective microorganisms with an antagonistic value of 50 or more (Eff
ctive Micro-organisms, below,
EM) and phytase.

Further, the phytin-degrading feed of the present invention has a koji mold (Asperg) instead of the effective microorganism group (EM).
illus Organizer) or Aspergillus niger (Asper)
gillus Awamori) is added.

As grain containing phytin, rice bran, corn, sesame and the like may be used. Further, a mixture of these raw materials may be used. In particular, when rice bran is used, the content of phytin is relatively large, and the absorption rate of inositol into the body of livestock is improved.

The EM refers to microorganisms existing in nature, such as lactic acid bacteria, yeasts, actinomycetes, and photosynthetic bacteria, which are effective in agricultural production,
Further, it is composed of enzymes as its metabolites and contains microorganisms of 80 genera of 10 genera or more, and among them, photosynthetic bacteria have a property of causing a microbial group to work in a certain direction (revival type). Such a variety of microorganisms coexist and coexist with each other using cereals as a medium, interlock with each other, and exert a synergistic effect.

The photosynthetic bacteria digest the ammonia, hydrogen sulfide, mercaptan and various hydrocarbons generated in the waste to eliminate the source of malodor and at the same time promote the production of various effective amino acids such as proline and methionine. Have. Moreover, when actinomycetes and yeast are used, a microbial flora of Gram-positive bacteria that suppresses the action of harmful microorganisms is formed.

The types of microorganisms present in the EM are those belonging to actinomycetes, for example, Strepto.
-Myces sp. (ATCC 3004), Str
eptoverticillium sp. (ATCC
23654), Nocardia sp. (ATCC
19247), Micromonospora s.
p. (ATCC 12452), Rhodococcu
s sp. , Belonging to photosynthetic bacteria, for example, R
hodopseudomonas sp. (R.sph
aeroldes), Rhodosprilillum
sp. (R. fulum), Chromatium s
p. (C. okenii), Chlorobium s
p. (C. limicola), lactic acid bacteria (lactic acid-producing bacteria)
Belongs to, for example, Lactobacillus
s sp. (IFO 3070), Propionib
acterium sp. (P. freudenrei
chii), Pediococcus sp. (P.h
alophilus), Streptococcus
sp. (S. lactis. S. faecalis),
As a filamentous fungus, for example, Aspergill
us sp. (RIFY 5770.RIFY 502
4), Mucor sp. (IFO 8567), which belongs to yeast, for example, Saccharomyce
s sp. (NRRL 1346, Y 977), Ca
ndida sp. (C. utilis).

The amount of EM added to the raw material grains is preferably about 0.2 W% of the bacterial cells with respect to the raw material grains. Alternatively, about 0.3 W% of the so-called EM blur, which is obtained by culturing EM in rice bran or the like, may be added.

The amount of phytase added is preferably about 1 to 2 W% of phytin in the raw material grain.

The antagonistic value for the EM is a scale that relatively expresses the degree of growth inhibition between microorganisms, and is evaluated by the inhibition circle measurement method to determine the growth. The case of no inhibition is represented as 0, the case of complete inhibition is represented as 100, and the middle thereof is represented as 50. Therefore, the term “antagonistic value of 50 or more” in the present invention means that the growth is relatively suppressed.

[0016]

[Function] In order to decompose phytin into inositol, it is necessary to cleave the ester group, and the enzyme that does this is phytase, but phytase usually has low enzymatic activity, and even if it is in the environment of the animal's body, The efficiency of its decomposition and conversion is extremely poor. However, when EM, or Aspergillus or Aspergillus oryzae is mixed therein, it is presumed that the cleavage of the ester group proceeds smoothly due to some interaction associated with the metabolic process. According to the experiment, the most favorable result was obtained in the EM having an antagonistic value of 50 or more, but the exact theory is awaiting future research and is not the main subject of the present invention.

[0017]

EXAMPLES Hereinafter, each example in which an experiment was conducted on the present invention will be described together with a comparative example. The guaranteed ingredient of the compounded feed (Kameya Shoji Co., Ltd. spawning mash) used in the experiment was crude protein 1
7.0%, crude fat 3.0%, crude fiber 6.0%, crude ash 1
4.0%, 2.6% calcium, 0.5% phosphorus,
A phytine-degrading feed was prepared by adding phytase, EM, etc. to this, and a predetermined amount of water was added to this, and the test was performed under anaerobic conditions in a thermostatic chamber in consideration of the environment such as the temperature inside the animal body and the PH value. .

[0018]

[Example 1 ... EM + phytase] The compounded feed 10
To 0 g, 0.2 ml of EM (standard stock solution manufactured by EM Research Institute of International Research Center for Natural Agricultural Research), 0.1 g of phytase (manufactured by Amano Pharmaceuticals KK), and 30 ml of water are added, and the mixture is kept at 35 ° C. for 10 hours. Held

[Comparative Example 1 ... EM only] Same E to 100 g of the same mixed feed
Add 0.2 ml of M and 30 ml of water, and add 10 at 35 ° C.
Held for hours.

[Comparative Example 123 ... phytase only] Same feed 10
0.1 g of the same phytase and 30 ml of water were added to 0 g, and the mixture was kept at 35 ° C. for 10 hours.

[Example 2 ... ... Aspergillus + phytase] The same mixed feed 100
0.1 g of the koji mold 1/100 diluted powder, 0.1 g of the same phytase and 30 ml of water were added to g, and the mixture was kept at 35 ° C. for 10 hours.

[Comparative Example 2 ... Only koji mold] To 100 g of the same mixed feed, 0.1 g of the koji mold 1/100 diluted powder and 30 ml of water were added, and the mixture was kept at 35 ° C for 10 hours.

[Example 3 ......... Koji mold + phytase] The same mixed feed 10
To 0 g, 0.1 g of Aspergillus niger 1/100 diluted powder, 0.1 g of the same phytase and 30 ml of water were added, and the mixture was mixed at 10 ° C.
Held for hours.

[Comparative Example 3 ... Only black koji mold] To 100 g of the same mixed feed, 0.1 g of black koji mold 1/100 diluted powder and 30 ml of water were added, and the mixture was kept at 35 ° C for 10 hours.

Inositol after decomposition was measured by gas chromatography in the above Examples 1 to 3 and the corresponding comparative example, and the results and the decomposition rate of phytin were obtained as shown in Table 1 below. According to this, in all Comparative Examples, the decomposition rate of phytin is low, and inositol was hardly obtained, whereas in Examples 1 to 3, phytin was decomposed at a high decomposition rate, and a relatively large amount of inositol was obtained. It was confirmed that Particularly good results were obtained in EM.

[0020]

[Table 1]

[0021]

As described above, according to the phytin-degrading feed of the present invention, the addition of a predetermined microorganism and phytase to cereals containing phytin makes it impossible to use it in the body of animals. It is possible to effectively use phytin in the grain as a livestock feed, which can significantly improve the feed efficiency. In addition, the concentration of phosphorus in manure discharged from facilities related to livestock can be reduced, which is very useful for improving environmental pollution.

Claims (2)

[Claims] 1. A cereal containing phytin, which produces lactic acid and butyric acid with anti-acidity, has no pathogenicity, and has an antagonistic value of 50.
A phytinolytic feed comprising the addition of the above-mentioned effective microorganisms and phytase. 2. The phytin-degrading feed according to claim 1, wherein koji mold or Aspergillus niger is added in place of the "effective microorganism group".