JP3365527B2 - Manufacturing method of organic fertilizer - Google Patents

Abstract

PURPOSE:To produce an organic fertilizer free from malodor, composed of flowable fine particles having excellent handleability, containing a large amount of nutrient components necessary for the growth of plant in an easily absorbable state and containing extremely large amount of valuable microorganisms such as actinomycete useful for inhibiting the proliferation of microorganisms harmful for the growth of plant. CONSTITUTION:This organic fertilizer is produced by using a mixture of (A) 50-80wt.% of an organic material having an arabinoxylan content of >=20wt.% and (B) 50-20wt.% of at least one kind of adsorbing mineral material or charcoal or a raw material composed mainly of the mixture as a fermentation raw material, subjecting the material to primary fermentation in the presence of one or more kinds of microorganisms selected from Aspergillus flavus, Aspergillus oryzae and Aspergillus sojae, adjusting the pH of the primary fermentation product to 6.0-7.5 and carrying out the secondary fermentation preferably at pH6.0-9.0 in the presence of one or more kinds of arabinoxylan-assimilating actinomycete of the genus Streptomyces, bacterium of the genus Pseudomonas or bacterium of the genus Bacillus.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention relates to a method for producing an organic fertilizer.
Regarding In detail, it has excellent fertilizer effect, and also has plant pathogens.
Produce organic fertilizer with high control effect against fungi in a short period of time
Regarding how to

[0002]

2. Description of the Related Art Chemical fertilizers which have been used frequently until now have problems such as poor growth and withering of plants due to concentration disturbance, soil loss due to continuous use, environmental pollution and the like although they have immediate effects. Therefore, as a solution to such defects of the chemical fertilizer, organic matter derived from animals such as chicken dung, pork dung, beef dung, bone meal, and organic matter derived from plants such as oil dregs, barley, rice straw, and deciduous leaves. Organic fertilizers made from raw materials are being reviewed.
However, these organic fertilizers may contain a large amount of easily decomposable sugars and the like and may easily cause a temporary oxygen deficiency state to deprive the soil of oxygen during the decomposition, which may be harmful to plant growth. Further, there is a drawback that it takes a long time (generally, 2 weeks to 3 months) for composting and has an odor.

In recent years, in order to promote composting of organic raw materials, a method by improving a fermentation apparatus or adding a microbial material has been developed. As an example, there is a known method of promoting composting by planting bacteria that decompose organic wastes discharged from food factories such as fisheries processing plants and brewing factories, which is an expensive fermentation reactor. Since it is necessary to ferment at an average temperature of 75 ° C. or higher,
The cost was high in terms of thermal efficiency and equipment, and the process was complicated. It has also been recognized that organic substances such as wheat bran and defatted rice bran are effective as raw materials for organic fertilizers, but since they contain a large amount of cellulose and hemicellulose that are difficult to decompose, they are used for composting. It takes a long time (usually about 40 days to 2 months), and adding a commercially available microbial material to promote the composting does not significantly shorten the composting period.

[0004]

The present invention aims to obtain an organic fertilizer having a high fertilizing effect and capable of being fertilized by a simple method in a short time from an organic substance containing a large amount of cellulose or hemicellulose, particularly an organic substance containing a large amount of arabinoxylan. I have continued my research. As a result, after adding a fermentation aid consisting of adsorptive mineral materials and / or charcoal to an organic substance rich in arabinoxylan and performing primary fermentation in the presence of a specific Aspergillus fungus, the pH was adjusted to a specific range. Then, by performing secondary fermentation in the presence of another specific fungus, it is possible to easily produce a highly fertilizing organic fertilizer in a short period of time, and the organic fertilizer obtained by this method is effective against plant pathogens. The present invention has been completed by discovering that it contains a large amount of actinomycetes and other useful microorganisms having antagonistic properties and also has an extremely excellent control action against phytopathogenic fungi.

That is, the present invention provides (A) 50 to 80% by weight of an organic substance having an arabinoxylan content of 20% by weight or more, and (B) at least one kind of an adsorptive mineral material and charcoal of 50 to 20% by weight. A mixture of, or
Using the mixture as a main component and adding an inorganic fertilizer component thereto as a fermentation raw material, (i) Aspergillus flavus, Aspergillus oryzae and Aspergillus
After primary fermentation in the presence of one or more species of bacteria consisting of soya, (ii) the presence of one or more species of Streptomyces actinomycetes, Pseudomonas spp. And Bacillus spp. It is a method for producing an organic fertilizer characterized by secondary fermentation below.

The organic substance (A) having an arabinoxylan content of 20% by weight (hereinafter, "% by weight" is simply referred to as "%") used in the present invention includes wheat bran (arabinoxylan content: usually about 30 to 40%). , Rice bran (about 35
45%), corn hulls (about 35 to 45% of the same), and the like, but not limited to these, and any organic substance having an arabinoxylan content of 20% or more can be used. The arabinoxylan-containing organic substance (A) may be used alone or in combination of two or more. If the arabinoxylan content in the organic matter is lower than 20%, an organic fertilizer containing a large amount of useful flora such as actinomycetes cannot be obtained, the content of water-soluble saccharides is too high, and composting does not proceed easily The fertilizer has an offensive odor. Here, in the present invention, the arabinoxylan content in the organic matter,
Tario, Bhatti et al. [Biochim. Biophys. Acta. 222
(1970) 339-347].

Among the component (B), examples of the adsorptive mineral material include mineral materials such as zeolite and vermiculite, which may be natural or synthetic. Further, examples of charcoal include charcoal, kun charcoal, chaffed charcoal, activated carbon, bone charcoal and the like. As the component (B), only one type of adsorptive mineral material and charcoal may be used, or two or more types may be used in combination. As the component (B), it is preferable to use two or more kinds in combination, and it is particularly preferable to use both the adsorptive mineral material and the charcoal. This component (B) improves the air permeability of the fermentation product and prevents excessive solidification, promotes the growth of useful microorganisms, improves the ability to retain useful nutrients produced by fermentation, and the like.

In the present invention, the arabinoxylan-containing organic substance (A) is contained in an amount of 80 to 50%, and the component is based on the total weight of the arabinoxylan-containing organic substance (A) and the component (B).
It is necessary to use (B) in a proportion of 20 to 50%. When the amount of the component (B) is less than 20%, fermentation is difficult to be promoted and it takes a long time for composting. On the other hand, when the amount of the component (B) exceeds 50%, the growth of useful actinomycetes and the like is reduced and a fertilizer containing a large amount of useful flora cannot be obtained. The amount of the arabinoxylan-containing organic matter (A) is 60 to
It is particularly preferred that the amount of component (B) at 70% is 40-30%.

In the present invention, even if fermentation is performed using a mixture consisting of the arabinoxylan-containing organic matter (A) and the component (B) alone as a fermentation raw material, or a mixture obtained by adding an inorganic fertilizer component to the mixture as needed. Fermentation may be performed using it as a fermentation raw material. Examples of inorganic fertilizer components that can be added include inorganic fertilizer components such as a nitrogen source such as ammonium sulfate and lime superphosphate, a phosphorus source, a potassium source, and an iron source. Even if only one type is added, Alternatively, two or more kinds may be added. The fertilizing effect of the fertilizer of the present invention can be enhanced by adding these inorganic fertilizer components. Further, when these inorganic fertilizer components are added, it is preferable that the amount is about 5% or less based on the total weight of the arabinoxylan-containing organic matter (A) and the component (B).

And, arabinoxylan-containing organic matter
(A) and component (B) and, if necessary, inorganic fertilizer component are further added and uniformly mixed to form a fermentation raw material. The fermentation raw material may be directly used for the primary fermentation as it is, but it is desirable to sterilize the bacteria to kill the bacteria before adding the bacteria for the primary fermentation. In the fermentation, it is necessary to adjust the water content of the raw material to one suitable for fermentation, and it is desirable to perform the sterilization treatment before heating with heated steam because it is possible to perform the sterilization treatment and the water content adjustment at the same time. .

In the present invention, the above fermentation raw material is used in the presence of one or more species of bacteria consisting of Aspergillus flavus, Aspergillus oryzae and Aspergillus sojae. First ferment below. Aspergillus flavus used for this primary fermentation,
For Aspergillus oryzae and Aspergillus soja, known bacteria may be used, and commercially available products and those that can be distributed can be obtained and used. Examples of Aspergillus flavus that are commercially available or available for sale include the Institute of Fermentation, O.
saka: IFO) IFO 30106, IFO 30107, IFO30180,
Institute of Applied
Microbiology, University of Tokyo: IAM) IAM 300
3, Department of Agricultural Chemistry, Faculty of Agriculture, Hokkaido University (Departmen
t of Agricultural Chemistry, Faculty of Agricultur
e, Hokkaido Univ .: AHU) AHU 7407, American Type
Examples include ATCC 1003 from Culture Collection, USA (ATCC). Further, as an example of Aspergillus oryzae commercially available or available for sale, IFO 30102 to 301
05, IAM 2648, IAM 2630, IAM 2609, AHU 7134, AHU 71
38, AHU 7139, AHU 7146, AHU 7156, AHU 7159, AHU 7
216 etc. can be mentioned. In addition, Aspergillus
Examples of soya include IFO 4200, IFO 4239, IFO 4241,
IFO 4243, IFO 4244, IFO 4252, IFO 4274, IFO 4279,
IFO 4386, IFO 4391, IFO 4403, IFO 5241, IFO 3011
2, IAM 2669, AHU 7181 and the like.

The amount of the bacteria used for the primary fermentation is preferably about 0.05 to 0.2% of the fermentation raw material in terms of dry cells. In this primary fermentation, the above-mentioned microbial cells for primary fermentation may be used as they are, or a pre-fermented product containing at least one of these microbial cells may be produced in advance and used. When the pre-fermented product is used, the arabinoxylan content of 20% by weight or more as described above organic matter 10
-50% by weight and 90-50% by weight of an adsorbent mineral material such as vermiculite or zeolite,
About 0.0 of at least one kind of the above-mentioned primary fermentation cells is used.
Add 5 to 0.2% by weight to increase the water content to 35 to 5
It is preferable that the pre-fermented product is prepared by adjusting the amount to 0% by weight and fermenting at a temperature of about 20 to 70 ° C., and using this as a bacterial cell material for primary fermentation. When the primary fermentation is carried out by adding the preliminary fermented material to the raw material for primary fermentation at a ratio of about 10 to 20% by weight, the organic fertilizer is kept stable throughout the year while preventing the invasion of various bacteria. Can be manufactured in.

The primary fermentation is usually aerobic fermentation, and it is necessary to control the water content in the raw material during fermentation. When the water content is too large, the heat of fermentation is less likely to be generated, and anaerobic fermentation mainly occurs to easily produce a spoilage substance having an offensive odor and a low fertilizing effect. On the other hand, when the water content is low, the fermentation temperature rapidly rises, which causes volatilization of the active ingredient, such as nitrogen, and death of useful microorganisms. Generally, the water content is about 35-50%, especially about 40-, based on the total weight of the fermented feedstock.
It is desirable to carry out primary fermentation at 45%. In order to carry out the primary fermentation uniformly, it is necessary to make the water content in the fermentation raw material uniform, so mix well. And, during the primary fermentation period, the temperature of the fermented product is preferably kept at about 20 to 75 ° C. Product temperature is 7
If it is higher than 5 ° C, volatilization of nitrogen content and the death of useful microorganisms are likely to occur, while if it is lower than 20 ° C, aerobic fermentation becomes insufficient, and usefulness of components in the arabinoxylan-containing organic matter (A) It may not be possible to achieve conversion to components or growth of useful microorganisms. As a specific method for maintaining the product temperature of the fermented product at about 20 to 75 ° C., the atmospheric temperature during fermentation is set to 2
It can be sufficiently achieved by controlling at 0 to 40 ° C, especially at 25 to 30 ° C.

In this primary fermentation, the fermentation raw material whose water content is adjusted is deposited, and the deposited fermentation raw material is cut back and stirred several times until the end of the primary fermentation so as to maintain the product temperature in the above temperature range. The end of the primary fermentation can be known by using the time when all the granules such as bran used as a raw material become aggregated or aggregated. The amount of water-soluble total sugar in the fermentation raw material at the start of fermentation is usually about 30 to 50 mg per 1 g of the raw material, but the amount of water-soluble total sugar in the fermentation raw material decreases as the primary fermentation proceeds. , The total amount of water-soluble sugar per gram of fermented product is about 2
It is preferable to finish the primary fermentation when the amount reaches 0 to 25 mg. Further, since the above-mentioned fungi added to the fermentation raw material grow at the end of this primary fermentation, the primary fermentation is completed when the total number of these bacteria is usually 1 × 10 ⁶ cells / g or more. You can also For example, 0.1% of the above fermentation raw material whose water content is adjusted to about 40%
When the above-mentioned dried primary fermentation fungus is added and the fermentation is carried out while controlling the ambient temperature to about 25 to 30 ° C, the primary fermentation is usually completed in about 30 to 80 hours.

The pH of the fermented product at the end of the primary fermentation is usually 4.
It is around 0 to 5.5, which is suitable for growth of filamentous fungi (Aspergillus spp.) Used for primary fermentation, but not suitable for fermentation of fungi used for the next secondary fermentation. In addition, if the pH of the fermented product is 5.5 or less, anaerobic conditions are likely to occur, which inhibits the activity of the secondary fermenting fungi that work under aerobic conditions, and allows the secondary fermentation to proceed smoothly as it is. Is difficult. Therefore, in the present invention, the pH of the fermented product after the primary fermentation is 6.0 to 7.5, particularly 6.5.
It is preferably adjusted to 7.0. For adjusting the pH, either a basic inorganic compound or a basic organic compound can be used. Among them, slaked lime is preferably used because it is economical and highly safe. The pH of the primary fermented product is 6.
In adjusting to 0 to 7.5, a method in which a basic compound such as slaked lime is added as it is in a solid state such as powder to the primary fermentation finished product and mixed, or a basic compound is dissolved or dispersed in a liquid such as water It is possible to use a method of adding and mixing to the primary fermentation finished product.

Next, the primary fermented product whose pH is adjusted to 6.0 to 7.5 is actinomycete of the genus Streptomyces capable of assimilating arabinoxylan, bacteria of the genus Pseudomonas, and Bacillus.
Secondary fermentation is carried out in the presence of one or more bacteria belonging to the genus, and at this time, the pH is 6.0 to 9.0, particularly 7.0.
It is preferable to carry out the secondary fermentation to 7.5. As the actinomycetes of the genus Streptomyces at that time, especially Streptomyces heimi (Streptomyc)
es heimi), Streptomyces flaveolus (Strept
omyces flaveolus), Streptomyces misionensis (Streptomyces misionensis), Streptomyces
Humanus (Streptomyces fumanus), Streptomyces albulus and Streptomyces griseofuscus
fungus such as ofuscus) is useful because of its high assimilation effect. Pseudomonas fluorescens (Pseudomonas) is an example of a useful bacterium of the genus Pseudomonas.
nas fluorescens), Pseudomonas putida
onas putida) and useful bacillus (Bacillu
s) bacteria include Bacillus circulans (B
acillus circulans), Bacillus subtilis
s subtilis) and the like.

As the actinomycetes of the genus Streptomyces, the bacterium of the genus Pseudomonas and the bacterium of the genus Bacillus used for this secondary fermentation, known ones may be used, and commercially available ones or those available for sale You can
Examples of Streptomyces heimi are IFO 13048, ATC
C 25460, IFO 3408, IFO 3715, IFO 12768, ATCC 3319 for Streptomyces flaveolus, and IFO 1306 for Streptomyces misionensis.
3, ATCC 14991, ATCC 25475, as examples of Streptomyces humanus IFO 13042, ATCC 19904, ATCC
25454, I as an example of Streptomyces albus
FO 13410, IFO 14147, ATCC 12757, and IFO 12870, ATCC 23 as an example of Streptomyces griseofuscus
916 can be mentioned. Examples of Pseudomonas fluorescens are IFO 13922, IFO 14160, IAM.
1092, IAM 1154, IFO 3738, IFO 12653, IF0 12996, IFO 13696, IFO 1 as examples of Pseudomonas putida
4164, IFO 14796, ATCC 8209, ATCC 12633 can be mentioned. Furthermore, as an example of Bacillus Circulance
IFO 3329, IFO 3967, IFO 13625-13640, IAM 1140, AH
U 1363, AHU 1365, AHU 1646, ATCC 4513, ATCC 8384,
ATCC 9966, ATCC 14175, ATCC 14176, examples of Bacillus subtilis IFO 13722, IFO 14117, IFO 1414
0, IFO 14144, IFO 14191, IFO 14192, IFO 14357, IFO
14411-14419, ATCC 19659, etc. can be mentioned.

The amount of the bacterium used for the secondary fermentation is preferably about 0.05 to 0.2% based on the weight of the product obtained by the primary fermentation in terms of dry cells. In this secondary fermentation, a fermented product containing at least one of the above-mentioned pure dry cells may be used in addition to the pure dry cells described above. For example, a part of the secondary fermentation product obtained by the secondary fermentation to be described below can be collected, and the secondary fermentation can be performed by adding the collected secondary fermentation product as a microbial cell for the secondary fermentation to the primary fermentation finished product. In that case, it is advisable to add about 5 to 20% of the secondary fermentation product of the lot produced before drying to perform the secondary fermentation of the lot produced next.

This secondary fermentation is also usually aerobic fermentation, and it is necessary to control the water content in the raw material and the fermentation temperature during fermentation. Too much or too little water causes the same problems as in primary fermentation, and too high or too low fermentation temperature results in the same poor results as in primary fermentation. Generally, the water content is about 30-45 based on the weight of the primary fermentation product.
%, Especially about 40-45%, and the product temperature is about 20-75
It is good to keep at ℃. A specific method for maintaining the product temperature may be the same as the control of the atmospheric temperature during the primary fermentation. At the time of the secondary fermentation, it is preferable to deposit the primary fermentation product whose water content has been adjusted, and cut back and agitate the deposit several times until the secondary fermentation is completed so as to maintain the product temperature in the above-mentioned temperature range. The end of the secondary fermentation can be known by obtaining a granular product with a fine particle size. More strictly, it is preferable to finish the secondary fermentation when the water-soluble total sugar amount per 1 g of the fermentation product is about 5 mg or less. Generally, the closer the amount of water-soluble total sugar in the secondary fermentation product or its extract is to zero, the higher the effect as an organic fertilizer and the more desirable.

During the secondary fermentation, the pH thereof tends to increase as the bacteria grow, and the pH tends to be 9 or more after 2 to 3 days of the secondary fermentation. However, if the pH is higher than 9, the growth of the above-mentioned fungi for secondary fermentation is hindered and the fermentation is apt to not proceed. Therefore, it is preferable to adjust the pH to 9 or less. In adjusting the pH, both weakly acidic inorganic compounds and organic compounds can be used. Of these, weakly acidic inorganic compounds are preferable, and weakly acidic phosphates such as monosodium phosphate are more preferable because they are easily available and cause less damage to plants. PH during secondary fermentation
In the adjustment, the pH of the secondary fermented product is continuously or intermittently measured, and when the pH is about to exceed 9, a weak acidic compound such as monosodium phosphate may be added and mixed into the fermented product, At that time, the weakly acidic compound may be added to and mixed with the fermented product in the course of secondary fermentation as it is in a solid state such as powder, or may be dissolved or dispersed in a liquid such as water and added and mixed. When a weakly acidic compound such as monosodium phosphate is used as the pH adjuster, the pH during the secondary fermentation is
It is preferable because it has a deodorizing effect together with the adjustment of.

When the secondary fermentation is carried out as described above, at the end of the secondary fermentation, the above-mentioned fungi added for secondary fermentation are growing in the fermentation product, and the total number of these bacteria is usually The secondary fermentation can be terminated when the concentration reaches 1 × 10 ⁷ cells / g or more. For example, to the primary fermentation product having a water content adjusted to 35%, the dried secondary fermentation fungus corresponding to 0.1% thereof is added to the ambient temperature of about 2%.
When the fermentation is controlled at 5 to 30 ° C, the secondary fermentation is usually completed in about 48 to 100 hours.

Therefore, according to the present invention, the water content in the raw material is set to about 35 to 50% and the ambient temperature is set to about 25 to 50%.
Under standard conditions where fermentation is controlled at 30 ° C,
Composting of arabinoxylan-containing organic matter can be achieved in about 78 to 180 hours in total from the primary fermentation. Further, the primary fermentation and the secondary fermentation are not limited to the method of cutting back or stirring the deposited raw material several times, and any method can be adopted as long as the aerobic fermentation of the raw material can be smoothly performed, for example, the raw material is constantly It can also be fermented with stirring.

By the way, Aspergillus flavus (As
pergillus flavus), Aspergillus oryzae (Aspe
rgillus oryzae) and Aspergillus soya (Aspe
It is widely known that bacteria such as rgillus sojae) are highly safe bacteria used for soy sauce koji, etc., and have extremely high protease activity and amylase activity and excellent protein and starch degrading performance. ing. However, according to the research conducted by the present inventors, the decomposition rate of organic substances containing cellulose and hemicellulose, particularly arabinoxylan, was low, and the arabinoxylan-containing organic substance could not be composted with only these bacteria. Further, when the arabinoxylan-containing organic matter is composted using these bacteria, filamentous fungi that inhibit the growth of plants remain as the dominant bacteria and cannot be used as fertilizers as they are. On the other hand, in the present invention, the primary fermentation products of these bacteria are treated with the actinomycetes of the genus Streptomyces, Pseudomonas (Ps).
Organic fertilizer with high fertilizing effect from arabinoxylan-containing organic matter which has been difficult to fertilize by secondary fermentation using one or more species of bacteria of the genus eudomonas and bacteria of the genus Bacillus It made it possible to manufacture in a short period of time.

The organic fertilizer of the present invention obtained after the secondary fermentation is used as it is, or it is dried sufficiently under the condition that useful microorganisms are not killed and then put in a moisture-proof bag or other container. Save it. The organic fertilizer of the present invention is a fine granular material having no bad odor and is extremely easy to handle. The organic fertilizer of the present invention can be effectively used for any plant such as flowers, vegetables, rice, wheat, beans, trees and fruit trees. Among them, the fertilizer of the present invention is particularly suitable as a lawn grass fertilizer. When applying to turfgrass, you can directly apply fertilizer to turfgrass by spraying, etc.
Usually, it is applied at a rate of about 50 to 500 g per 1 m ^{2 of} turfgrass.
Further, instead of the grass soil of the grass, about 100 g per 1 m ² of grass.
When applied at a rate of up to 1000 g, the effect of soil and the effect of fertilization can be obtained at the same time.

[0025]

【Example】

<< Example 1 >> 50 kg of wheat bran with an arabinoxylan content of 35%, commercially available charcoal 14.3 kg, zeolite 3.
Mixture consisting of 6 kg and 3.6 kg vermiculite (total 71.5 kg: wheat bran content 70 in the mixture)
%) Was uniformly mixed using a large-scale mixer, 27.1 kg of water was added, and sterilization treatment was performed with heated steam. The water content of the mixture after sterilization was about 40%. Next, Aspergillus oryzae (IFO 30
After adding 70 g of the dried bacterial cells of 105) and mixing them uniformly, the atmospheric temperature was controlled at 27 ° C.
Primary fermentation was carried out for 8 hours. During the primary fermentation, cutting was performed twice. Then Streptomyces flaveolus (St
reptomyces flaveolus) (IFO12768) and Bacillus circulans (IFO 13640)
70 g of each was added and mixed uniformly, and secondary fermentation was carried out for 72 hours while controlling the atmospheric temperature at 25 ° C. under aerobic conditions. During the secondary fermentation period, cutting was repeated twice to make the temperature uniform. The number of bacteria and the amount of total water-soluble sugar in the fermentation product (organic fertilizer) obtained after the completion of secondary fermentation were measured by the following methods.

<Method of measuring the number of bacteria> The number of bacteria was measured by the diluted agar plate culture method as follows. General viable cell count : When cultured at 37 ° C. for 24 hours using a standard agar plate medium (pH 7.0) prepared by adding 5 g of polypeptone, 2.5 g of yeast extract, 1 g of glucose and 15 g of agar per liter of water. The number of colonies was measured. Number of filamentous fungus : Colony when cultured at 37 ° C for 3 days using MYG agar plate medium (pH 4.3) prepared by adding 20 g of malt extract, 5 g of yeast extract, 10 g of glucose and 25 g of agar per liter of water. The number was measured. Actinomycetes : starch 10 g, casein 0.
_{3g, KNO 3 2g, NaCl 2g} , K 2 HPO 4 2g, MgSO 4 · 7H
₂ O 0.05 g, CaCO ₃ 0.02 g, FeSO ₄ .7H ₂ O 0.
Agar plate medium prepared by adding 01 g, agar 18 g and cycloheximide (antifungal agent) 0.06 g (pH
The number of colonies when cultured for 6 days at 30 ° C. was measured using 7.2).

<Measurement of Total Sugar Content> According to the method described in Michinori Nishio, “Basic Knowledge of Soil Microorganisms”, pp. 74-75, published by Noyama Fishing Village Cultural Association (1989), 7
1 g of the fermented product after the day is collected, put in 30 ml of water, stirred and left at 60 ° C. for 3 hours. Then Toyo Filter Paper N
0.5 ml of a 5% aqueous phenol solution and 3 ml of concentrated sulfuric acid are added to 0.5 ml of the resulting filtrate.
And stir. After leaving this for 30 minutes,
The absorbance at 90 nm was measured to determine the total sugar amount (mg) as a glucose equivalent amount. Table 1 shows the number of bacteria, the total sugar content and the properties of the fermentation product obtained in Example 1.

Example 2 An organic fertilizer was produced in the same manner as in Example 1 except that 50 kg of defatted rice bran was used instead of wheat bran, and the number of bacteria and the total sugar content in the produced organic fertilizer were determined in Example. The measurement was performed in the same manner as in 1. The results are shown in Table 1 together with the properties of the organic fertilizer obtained in this Example 2.

Example 3 Example 10 was repeated except that 10 kg of the fermentation product obtained after the secondary fermentation of Example 1 was used in place of the cells for secondary fermentation in Example 1 to carry out secondary fermentation. An organic fertilizer was produced in the same manner as in Example 1, and the number of bacteria and the total sugar content in the produced organic fertilizer were measured in the same manner as in Example 1. The results are shown in Table 1 together with the properties of the organic fertilizer obtained in this Example 3.

<< Control Example 1 >> Wheat bran 50 of Example 1
Soybean residue (arabinoxylan content 0%) instead of kg
A fermentation product was produced by carrying out primary fermentation and secondary fermentation in the same manner as in Example 1 except that a mixture of 22.5 kg and wheat bran (arabinoxylan content 35%) 27.5 kg in a total of 50 kg was used. The number of bacteria and the total sugar amount in the obtained fermentation product were measured in the same manner as in Example 1. The results are shown in Table 1 together with their properties.

Comparative Example 2 The number of bacteria and the amount of total sugar in a commercially available fermented fertilizer for turfgrass, which was produced by using organic substances derived from food production raw materials, were measured in the same manner as in Example 1. The results are shown in Table 1.

[0032]

[Table 1]

From the results shown in Table 1, Examples 1 to 3 of the present invention using an organic substance having an arabinoxylan content of 20% or more.
Shows that the actinomycetes, which are useful bacteria, are selected and proliferated in a large amount as compared with Control Examples 1 and 2 in which an arabinoxylan content is less than 20% or an organic substance containing no arabinoxylan is used. . Furthermore, from the results of Table 1, in Examples 1 to 3 of the present invention, the amount of total sugar in the fermentation product was small and composting was in progress, whereas in Comparative Example 1
It can be seen that in Nos. 2 to 3, the total amount of sugar in the fermentation product is large and composting is not progressing.

Example 4 71.5 kg of a mixture containing each component in the ratio (%) shown in Table 2 was prepared in 5 sections and used as a fermentation raw material. As test cells for secondary fermentation, Streptomyces misionensis (Strept
70 g of dried cells of omyces misionensis) (IFO 13063)
Was used in Test Group 5, and secondary fermentation was carried out without adding bacteria. Otherwise, primary fermentation and secondary fermentation were carried out in the same manner as in Example 1 to produce a fermentation product. The total sugar amount and the number of actinomycetes of the fermentation product obtained in each test section were measured in the same manner as in Example 1. The results are shown in Table 2.

[0035]

[Table 2]

From the results shown in Table 2, the higher the content of the adsorptive mineral material and the carbonaceous materials in the fermentation raw material, the smaller the total sugar amount in the fermentation product and the more the composting progressed. Is less than 20% or more than 50%, the number of actinomycetes, which is a useful bacterium, is small, and it is clear that the total amount of adsorptive minerals and charcoal is 20 to 50%.
Furthermore, from the results of Table 2, it is understood that when the Streptomyces mycionensis bacterium is not added during the secondary fermentation, the secondary fermentation does not proceed and composting is difficult.

Example 5 The organic fertilizers of the present invention of Examples 1-3, the organic fertilizers of Control Examples 1-2 and commercially available chemical fertilizers (Chiso: phosphorus: potassium = 8%: 8%: 8%) were used. Using this, a growth test on bent grass was performed as follows. Seven test plots of 2 m ² per plot were prepared in a general field, and seeds of bent turf were sowed and cut to a plant height of 30 mm when they had grown to some extent. Here, each of the organic fertilizers of Examples 1 to 3 of the present invention is applied to the first to third test sections, and each of the organic fertilizers of Comparative Examples 1 to 2 is applied to the fourth to fifth test sections. , And the sixth test section was applied with commercially available chemical fertilizer such that the nitrogen content of each fertilizer was 3 g / m ² . Also, for comparison, fertilizer was not applied to the seventh test section. 15, 30, 45 and 60 days after fertilization, the grass of each section was cut to a grass height of 20 mm. Thirty
The fresh weight of the mowed turfgrass after day and 60 days was measured. The degree of green leaf of the turfgrass at that time was evaluated by the following.

Evaluation of the degree of green leaf of turfgrass The degree of greenness of the leaf of turfgrass was visually observed,
According to the evaluation criteria shown in Table 3 below, yellow-brown to dark green were divided into 5 grades and evaluated. The higher the number, the darker the green.

[0039]

[Table 3] Evaluation criteria for the degree of green leaf of turfgrass 1 ... fairly yellow and brownish green with nitrogen deficiency 2 ... slightly yellowish green than 3 3 ... average turfgrass green, moderate nitrogen application 4 ... 3 more Slightly darker green 5 ... 3, which is much greener than 3 and shows excess nitrogen The above results are shown in Table 4 below.

[0040]

[Table 4]

From the results in Table 4 above, in the case of test sections 1 to 3 in which the organic fertilizer of the present invention of Examples 1 to 3 was applied to turfgrass, test section 7 without fertilizer, Control Example 1 or 2
It can be seen that both the growth of turfgrass and the degree of green leaf thereof are better than those of the test sections 4 and 5 in which the fertilizer of No. 1 was applied. In addition, test sections 1 to 3 to which the organic fertilizer of the present invention is applied
Shows that the growth immediately after application is not too large as compared with the test section 6 to which the chemical fertilizer is applied, the green leaf degree is not too dark and the light green color is good, and further, it is excellent in terms of the sustainability of fertilization. You can see that

Example 6 Arabinoxylan content 30
% Wheat bran 50 kg, commercial vermiculite 20
kg and 5 kg zeolite (75 total)
(kg: wheat bran content of 67% in the mixture) was uniformly mixed using a large-scale mixer, 40 kg of water was added, and sterilization was performed by heating steam. The water content of the mixture after sterilization was about 45%. Next, dried cells 7 of Aspergillus oryzae (IFO 30105)
After adding 0 g and uniformly mixing, the atmosphere temperature was controlled at 27 ° C., and the mixture was cut twice under aerobic conditions and subjected to primary fermentation for 72 hours. When the pH of the fermentation product at the end of this primary fermentation was measured, it was 5.1.
To the primary fermentation product, 1 kg of slaked lime was added to adjust the pH to 6.5. The pH was measured by using a soil pH measuring device (manufactured by Fujiwara Seisakusho; “PHS-33 type”).

Next, Streptomyces flaveolus (IFO 12768) and Bacillus subtillus (IFO 13722)
And Pseudomonas putida
70 g of each of the bacteria (IFO 3788) was added and uniformly mixed, and secondary fermentation was carried out for 96 hours while controlling the atmospheric temperature at 25 ° C. under aerobic conditions. During the secondary fermentation period, cut the water once every 24 hours and adjust the water content to 40%.
% To keep the temperature uniform. During the secondary fermentation,
The pH of the fermented material was continuously measured by the above-mentioned pH measuring device, and when the pH exceeded 9, monosodium phosphate 1k
g was added to lower the pH to 8. The number of bacteria and the amount of total water-soluble sugar in the fermentation product (organic fertilizer) obtained after the completion of the secondary fermentation were measured by the above-mentioned method. This Example 6
Table 5 shows the bacterial count, total sugar content and properties of the fermentation product obtained in
Shown in.

Example 7 An organic fertilizer was produced in the same manner as in Example 6 except that 50 kg of defatted rice bran was used instead of wheat bran, and the number of bacteria and the total amount of sugar in the produced organic fertilizer were determined in Example. The measurement was performed in the same manner as in 6. The results are shown in Table 5 together with the properties of the organic fertilizer obtained in this Example 7.

Example 8 Example 10 was repeated except that 10 kg of the fermentation product obtained after the secondary fermentation of Example 6 was used in place of the cells for secondary fermentation of Example 6 to perform secondary fermentation. An organic fertilizer was produced in the same manner as in Example 6, and the number of bacteria and the total sugar amount in the produced organic fertilizer were measured in the same manner as in Example 6. The results are shown in Table 5 together with the properties of the organic fertilizer obtained in this Example 8.

<Control Example 3> Wheat bran 50 of Example 6
Soybean residue (arabinoxylan content 0%) instead of kg
A fermentation product was produced by carrying out primary fermentation and secondary fermentation in the same manner as in Example 6 except that a total of 50 kg of a mixture of 22.5 kg and wheat bran (arabinoxylan content 35%) 27.5 kg was used. The number of bacteria and the total amount of sugar in the obtained fermentation product were measured in the same manner as in Example 6. The results are shown in Table 5 together with their properties.

Comparative Example 4 The number of bacteria and the total amount of sugar in a commercially available fermented fertilizer for turfgrass produced by using organic substances derived from food production raw materials were measured in the same manner as in Example 6. The results are shown in Table 5.

[0048]

[Table 5]

From the results shown in Table 5, Examples 6 to 8 of the present invention using an organic substance having an arabinoxylan content of 20% or more.
In comparison with Comparative Examples 3 to 4 in which the arabinoxylan content is less than 20% or an organic substance containing no arabinoxylan is used, it is found that actinomycetes, which are useful bacteria, are selectively propagated and contained in large amounts. . Further, from the results of Table 5, in Examples 6 to 8 of the present invention, the amount of total sugar in the fermented product was small and composting proceeded, while Comparative Example 3
It can be seen that in Nos. 4 to 4, the amount of total sugar in the fermentation product is large and composting is not progressing.

Example 9 71.5 kg of a mixture containing each component in the ratio (%) shown in Table 6 was prepared in 5 sections and used as a fermentation raw material. In addition, as cells for secondary fermentation, Streptomyces misionensis (Stre
ptomyces misionensis) (IFO 13063) dried cells 70
g was used, and in the test section 10, secondary fermentation was carried out without adding bacteria. Other than that, the fermentation product was manufactured by carrying out the primary fermentation and the secondary fermentation in the same manner as in Example 6. The total sugar amount and the number of actinomycetes of the fermentation product obtained in each test section were measured in the same manner as in Example 6. The results are shown in Table 6.

[0051]

[Table 6]

From the results shown in Table 6, the higher the content of the adsorptive mineral material in the fermentation raw material, the smaller the total amount of sugar in the fermentation product and the progress of composting.
When it is less than 0% or more than 50%, the number of actinomycetes, which is a useful bacterium, is small, and it is understood that the amount of the adsorbent mineral is appropriately 20 to 50%. Furthermore, from the results of Table 6, it is understood that when Streptomyces mycionensis bacteria are not added during the secondary fermentation, the secondary fermentation does not proceed and composting is difficult.

Example 10 The organic fertilizers of the present invention of Examples 6-8, the organic fertilizers of Control Examples 3-4 and commercially available chemical fertilizers (Chisso: phosphorus: potassium = 8%: 8%: 8%) were used. Using this, a growth test on bent grass was performed as follows. Seven test plots of 2 m ² per plot were prepared in a general field, and seeds of bent turf were sowed and cut to a plant height of 30 mm when they had grown to some extent. Here, each of the organic fertilizers of the present invention of Examples 6 to 8 was used for the first to third test sections, and Control Examples 3 to 4 were used for the fourth to fifth test sections.
Each of the organic fertilizers in Example 6 and the sixth test section were applied with commercially available chemical fertilizers so that the amount of nitrogen in each fertilizer was 3 g / m ² . Also, for comparison, fertilizer was not applied to the seventh test section. 15, 30, 45 and 60 days after fertilization
After a day, the grass of each section was cut to a grass height of 20 mm. Three
The fresh weight of the mowed grass was measured after 0 and 60 days. In addition, the degree of green leaf of the turf grass at that time was evaluated according to the evaluation criteria shown in Table 3 above. The results are shown in Table 7 below.

[0054]

[Table 7]

From the results of Table 7 above, in the case of test sections 1 to 3 in which the organic fertilizer of the present invention of Examples 6 to 8 was applied to turfgrass, test section 7 without fertilizer, Control Example 3 or It can be seen that both the growth of turfgrass and the degree of green leaf thereof are better than those of the test sections 4 and 5 in which the fertilizer of No. 4 was applied. In addition, test sections 1 to 3 to which the organic fertilizer of the present invention is applied
Shows that the growth immediately after application is not too large as compared with the test section 6 to which the chemical fertilizer is applied, the green leaf degree is not too dark and the light green color is good, and further, it is excellent in terms of the sustainability of fertilization. You can see that

<< Example 11 >> [Test for suppressing disease occurrence against fairy ring (turf grass disease)] (1) 50 m ² of bent grass was treated with the organic fertilizer (fermentation product) of the present invention obtained in Example 6 for 30 days 100g each
/ M ² (nitrogen amount 1.7 g / m ² ) was sprayed (invention group). (2) Instead of the organic fertilizer of the present invention, a commercially available chemical fertilizer (Chiso: phosphorus: potassium = 8%: 8%: 8%) was sprayed every 30 days so that the nitrogen content was the same as in the invention zone. (Control area). (3) Fairy ring lesions (clear circular lesions) naturally occurring on 50 m ² of turfgrass in the invention group of the above (1) and the control group of (2) were applied at the start of fertilization, 30 days later, and 60
Table 8 below shows the number of lesion occurrence spots visually observed after 90 days and 120 days. In the control area, 90 days after the start of fertilization, fairy ring lesions occurred in 8 places. Therefore, the organic fertilizer of the present invention obtained in Example 6 was used at a rate of 150 g / m ^{2 in 4} of them. When the chemical fertilizer was sprayed on the remaining 4 spots as it was in the same manner as in (2) above, as shown in Table 8 below, in the portion where the organic fertilizer of the present invention was sprayed, 4 spots were spread. While 2 of the Fairy ring lesions disappeared, none of the 4 Fairy ring lesions disappeared in the portion where the chemical fertilizer was applied.

[0057]

[Table 8] Number of Fairy Ring lesions (location) Start of fertilization 30 days later 60 days later 90 days later 120 days later Invention Zone 0 0 0 0 0 Control area 0 0 8 8 (A) (4 places) 2 (B) (4 places) 4 A: The organic fertilizer of the present invention obtained in Example 6 is sprayed. B: The chemical fertilizer is sprayed.

From the results shown in Table 8 above, in the invention zone to which the organic fertilizer of the present invention was applied, there was no occurrence of fairy ring, and by regularly applying the organic fertilizer of the present invention, the fairy ring disease caused by turfgrass disease was observed. It can be seen that the occurrence of fairy rings can be effectively prevented, and that even if fairy rings occur, the organic fertilizer of the present invention can be applied to eliminate the fairy rings and recover the grass from the fairy rings.

<< Example 12 >> [Test of disease suppressive effect against dollar spot (turfgrass disease)] (1) Bent turf 50 m ² was treated with 100 g / m of the organic fertilizer of the present invention obtained in Example 6 every 30 days. ² (nitrogen amount 1.7 g / m ² ) was sprayed (invention group). (2) Instead of the organic fertilizer of the present invention, a commercially available chemical fertilizer (Chiso: phosphorus: potassium = 8%: 8%: 8%) was sprayed every 30 days so that the nitrogen content was the same as in the test plot. (Control area). (3) Observing the disease status of the dollar spots naturally occurring in 50 m ² of turfgrass in the invention section of (1) and the control section of (2) at the start of fertilization, after 30, 60 and 90 days with the naked eye, Evaluation was performed according to the evaluation criteria shown in Table 9 below. The results are shown in Table 10 below.

[0060]

[Table 9]         [Evaluation criteria for disease status of dollar spots] Rating: Disease state   4: No illness   3: There are slight lesions (less than 10% of leaves) on the leaves of turfgrass   2: Disease spots are found on more than 10% and less than 20% of the leaves of turfgrass   1: Disease spots are found on more than 20% of the leaves of turfgrass

[0061]

[Table 10] Dollar spot disease situation fertilization at the start of 30 days after 60 days after 90 days after Invention Zone 4 3 2 3 Control Zone 4 2 1 1

[0062]

According to the method of the present invention , (A)
Organic matter having a vinoxylan content of 20% by weight or more
80% by weight and (B) of adsorptive mineral material and charcoal
Mixture consisting of 50 to 20% by weight of at least one of
Or a mixture of these and mainly inorganic fertilizer components
With added through as fermentation material, complex and without the use of time-consuming process and apparatus, in a simple low energy consumption, a short period of time about four days to 1 week, organic
Quality fertilizer can be produced. Obtained by the method of the present invention
It is manure can persist fertilizer effect without causing growth failure and withering of plants with such concentration disorders as conventional fertilizer causes very well growing plants. Those of the present invention
The organic fertilizer obtained by the method is particularly suitable for turfgrass, and even if it is continuously used, problems such as soil thinning and environmental pollution do not occur. In the organic fertilizer obtained by the method of the present invention, since the components of the arabinoxylan-containing organic matter are converted into the more effective components as fertilizers by fermentation, the temporary oxygen-deficient state and methane gas and ammonia as in conventional organic fertilizers are used. It does not cause plant growth failure or root weakness due to gas generation.

In the present invention, since at least one of adsorptive minerals and charcoal is contained in a proportion of 20 to 50% of the total amount of fermentation raw materials, fermentation is promoted and at the same time useful bacteria, especially arabinoxylan, are added. Promotes the growth of assimilating actinomycetes. As a result, the organic fertilizer of the present invention contains a large amount of nutrients required for plant growth in a form that is easily absorbed. Moreover, organic <br/> machine fertilizer obtained by the method of the present invention, contain the actinomycetes and other useful microorganisms has a function to prevent the breeding and habitat of harmful pathogens for the growth of plants plethora Therefore, it is possible to effectively control phytopathogenic fungi and to grow plants robustly and satisfactorily. The organic fertilizer obtained by the method of the present invention is extremely easy to handle since it has no offensive odor and is composed of fine particles that are smooth, and does not give off a bad odor even when fertilized.

Front page continuation (72) Inventor Hideaki Yamada 13 Okubo, Tsukuba, Ibaraki Nisshin Seifun Co., Ltd. Tsukuba Laboratories (72) Inventor Kaoru 5-3 Tsurugaoka, Oi-cho, Iruma-gun, Saitama Nisshin Seifun Co., Ltd. Company Food Research Laboratory (72) Inventor Koji Murakami 13 Okubo, Tsukuba City, Ibaraki Prefecture Nisshin Seifun Co., Ltd.Tsukuba Research Institute (72) Inventor Nao Okayama 13 Okubo, Tsukuba City, Ibaraki Nisshin Seifun Co., Ltd. 72) Inventor Kenzo Okada 13 Okubo, Tsukuba City, Ibaraki Nisshin Seifun Co., Ltd. (56) References JP-A-4-209787 (JP, A) JP-A-2-48487 (JP, A) JP 50-51857 (JP, A) JP-A-6-107511 (JP, A) JP-A-6-107512 (JP, A) JP-A-6-279162 (JP, A) (58) Fields investigated (Int .Cl. ⁷ , DB name) C05B 1/00-C05G 5/00

Claims (2)

(57) [Claims] 1. The content of (A) arabinoxylan is 20% by weight.
50 to 80% by weight of the above organic substances, and (B) at least one kind of the adsorbent mineral material and the charcoal 50 to 2
0% by weight of the mixture, or the mixture as the main component
Using a mixture of these with an inorganic fertilizer component as a fermentation raw material, (i) Aspergillus flavus, Aspergillus
After primary fermentation in the presence of one or more of the bacteria consisting of Orise and Aspergillus soya,
(ii) A method for producing an organic fertilizer, which comprises secondary fermentation in the presence of one or more of Streptomyces actinomycetes, Pseudomonas bacteria and Bacillus bacteria. 2. The pH of the product after primary fermentation is 6.0 to 7.5.
1 or 2 of Streptomyces actinomycetes, Pseudomonas bacteria and Bacillus bacteria.
The method for producing an organic fertilizer according to claim 1, wherein the secondary fermentation is carried out under the condition of pH 6.0 to 9.0 in the presence of one or more species.