CN116898896A - Traditional Chinese medicine composition for improving lactation yield of lactating sows and preparation and application thereof - Google Patents
Traditional Chinese medicine composition for improving lactation yield of lactating sows and preparation and application thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
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- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
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Abstract
The invention relates to a traditional Chinese medicine composition for improving lactation yield of sows in lactation period, and preparation and application thereof, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: the traditional Chinese medicine comprises the following components: 6-8 parts; bean pulp: 1-3 parts; glucose: 1-3 parts; bacillus subtilis: 1-3 parts; lactobacillus plantarum: 8-10 parts; cellulase: 3-5 parts; pectase: 5-7 parts. After the traditional Chinese medicine composition with the synergistic fermentation of the bacterial enzymes is used for the sows in the lactation period, compared with the traditional Chinese medicine without fermentation and the traditional Chinese medicine fermented by single bacteria, the daily average lactation amount and the nest average weight and the survival rate of the lactating piglets can be effectively improved.
Description
Technical Field
The invention relates to the field of biological fermentation, in particular to a traditional Chinese medicine composition for improving lactation yield of sows in lactation period, and preparation and application thereof.
Background
In order to ensure the normal development of livestock and poultry raising industry, the development of novel, green and pollution-free feed additives for replacing antibiotics has become a trend. The traditional Chinese medicine which is used as a medicine and food homology is a natural feed additive, is not easy to generate drug resistance, and is an ideal choice for preventing and treating animal diseases and replacing antibiotics. The probiotics microecological preparation can directly influence the microbial flora structure of animal intestinal tracts, inhibit the growth of harmful bacteria, strengthen the immunity of organisms, and relieve the problems of animal stress and the like.
The lactation period of the lactating sows is generally 30-42 days, and the lactation amount of the sows in the lactation period and the nutritional ingredients in the milk have great influence on the growth and development, survival rate and weight of the lactating piglets. Therefore, to improve the lactation capacity of the sow and ensure the growth and development of the piglet, so as to obtain higher survival rate of the piglet and weight of the piglet, and simultaneously, the sow can maintain a certain fat condition in the later period of lactation, so that the sow can normally estrus and ovulate, and the improvement of the lactation capacity of the sow in the lactation period and the nutrition components of milk are particularly important.
In order to ensure the normal development of livestock and poultry raising industry, the development of novel, green and pollution-free feed additives for replacing antibiotics has become a trend. The traditional Chinese medicine which is used as a medicine and food homology is a natural feed additive, is not easy to generate drug resistance, and is an ideal choice for preventing and treating animal diseases and replacing antibiotics. At present, a plurality of traditional Chinese medicine formulas for improving the immunity and lactation capacity of sows in lactation period have slow onset of action and insignificant effect, wherein the main reasons are related to the fact that the preparation method of the traditional Chinese medicine formulas can not reach the maximum efficacy; the fermented traditional Chinese medicine has wide application prospect, the traditional Chinese medicine is fermented to degrade substances such as cellulose, hemicellulose and the like in cell walls and cell interstitial under the action of microorganisms and enzymes thereof, so that the biological transformation of the traditional Chinese medicine is promoted, the cell walls are broken, the cell gaps are increased, the resistance of the active ingredients in the cell to spread outside the cell is reduced, the release, absorption and utilization of the active ingredients are promoted, and researches prove that the drug effect of the fermented traditional Chinese medicine can be improved by 8-10 times. Not only avoids the damage of the thermosensitive components of the traditional Chinese medicine in the traditional steaming and extracting process, but also improves the bioavailability of the effective components of the traditional Chinese medicine, thereby improving the antibacterial and antiviral capability of organisms. The fermented traditional Chinese medicine preparation can directly influence the microbial flora structure of animal intestinal tracts, inhibit the growth of harmful bacteria, strengthen the organism immunity, relieve the problems of animal stress and the like, and the fermentation process is added with the high-activity enzyme preparation, and the bacteria and the enzymes are used for synergistic fermentation, so that the nutrition value of the fermented traditional Chinese medicine can be improved, and the fermented traditional Chinese medicine preparation has remarkable effects on improving the activity of the probiotic flora, promoting the growth and digestion of animal organisms, enhancing the immunity and the like.
Disclosure of Invention
The invention aims to provide a traditional Chinese medicine composition for improving lactation yield of sows in lactation period, and preparation and application thereof, and the traditional Chinese medicine composition can effectively improve average daily lactation yield and nest average weight and survival rate of piglets after being used as a traditional Chinese medicine feed additive through synergistic fermentation of the bacterial enzymes and compared with traditional Chinese medicines without fermentation and traditional Chinese medicines through single bacterial fermentation.
The aim of the invention can be achieved by the following technical scheme:
a traditional Chinese medicine composition for improving lactation yield of lactating sows is prepared from the following raw materials in parts by weight:
the traditional Chinese medicine comprises the following components: 6-8 parts;
bean pulp: 1-3 parts;
glucose: 1-3 parts;
bacillus subtilis: 1-3 parts;
lactobacillus plantarum: 8-10 parts;
cellulase: 3-5 parts;
pectase: 5-7 parts.
Further, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
chinese angelica root: 10-12 parts;
radix Scutellariae Baicalensis: 9-11 parts;
semen Vaccariae: 10-12 parts;
medulla Tetrapanacis: 4-6 parts;
fructus trichosanthis: 2-4 parts;
herba Lycopi: 3-5 parts.
The functions of the components in the traditional Chinese medicine are as follows:
the Chinese angelica and astragalus root have the functions of enhancing organism metabolism, strengthening heart, dilating blood vessels, promoting hematopoiesis of hemoglobin and enhancing organism immunity, and simultaneously have the functions of bidirectionally regulating uterus, and have the functions of anti-inflammatory, antibacterial and diuretic; the compatibility of the semen vaccariae, the medulla Tetrapanacis, the snakegourd fruit and the herba lycopi has the effects of stimulating menstrual flow and promoting lactation and increasing lactation capacity.
Further, the glucose is anhydrous glucose.
The invention also provides a preparation method of the traditional Chinese medicine composition for improving lactation yield of sows in lactation period, which comprises the following specific steps:
s1, crushing the traditional Chinese medicine components in the technical scheme, and mixing the crushed traditional Chinese medicine components with bean pulp and glucose to prepare a solid culture medium;
s2, adding bacillus subtilis and lactobacillus plantarum into the solid culture medium obtained in the step S1, and then fermenting to obtain a culture medium mixture;
s3, adding cellulase and pectase into the culture medium mixture obtained in the step S2, and then fermenting to obtain the traditional Chinese medicine composition.
In step S1, the traditional Chinese medicine components are crushed and then pass through a 80-100 mesh screen, and then are mixed with soybean meal and glucose to prepare a solid culture medium.
Further, in step S1, the traditional Chinese medicine components are added according to the weight ratio: bean pulp: anhydrous glucose=6 to 8:1 to 3:1 to 3.
Further, in step S2, lactobacillus plantarum is inoculated into an MRS liquid medium to obtain lactobacillus plantarum fermentation broth, bacillus subtilis is inoculated into an LB liquid medium to obtain bacillus subtilis fermentation broth, and lactobacillus plantarum fermentation broth and bacillus subtilis fermentation broth are inoculated into a solid medium.
The plant milk stick further comprisesThe number of viable bacteria in the bacterial fermentation liquid is not less than 1 multiplied by 10 8 The number of viable bacteria in the bacillus subtilis fermentation broth is not less than 1 multiplied by 10 8 。
The total inoculation amount of the lactobacillus plantarum fermentation liquor and the bacillus subtilis fermentation liquor is 10%.
The bacillus subtilis fermentation liquid is prepared by the following steps of: lactobacillus plantarum broth = 1-3: 8-10, preferably bacillus subtilis fermentation broth: lactobacillus plantarum broth = 1:9.
further, in step S2, the fermentation temperature is 37 ℃, and the fermentation time is 60-84 hours, preferably 72 hours.
Further, in step S3, the medium mixture is prepared by: cellulase: pectase=5 to 7:1 to 3:2 to 4.
Further, in the step S3, the enzyme activity of the cellulase is 72U/g, and the enzyme activity of the pectase is 108U/g.
Further, in step S3, the fermentation temperature is 37℃and the fermentation time is 60 to 84 hours, preferably 72 hours.
In addition, the invention also provides application of the traditional Chinese medicine composition for improving lactation yield of the sows in the lactation period, and the traditional Chinese medicine composition is added into the sow feed.
Further, the traditional Chinese medicine composition is prepared according to the following formula 1:1000 is added into sow feed for feeding.
Compared with the prior art, the invention has the following beneficial effects:
1. according to the invention, fermentation strains and enzymes are added according to a specific proportion for synergistic fermentation, so that the content of crude polysaccharide in the traditional Chinese medicine prescription is improved, the drug effect of the traditional Chinese medicine prescription is improved, and meanwhile, the traditional Chinese medicine resources are saved, so that the nutrition matters in the traditional Chinese medicine components are fully utilized;
2. the invention utilizes bacillus subtilis to ferment traditional Chinese medicine, can synthesize enzymes such as a-amylase, protease, cellulase and the like for fermentation, secrete active substances such as subtilisin, polymyxin, antibacterial peptide and the like, and inhibit pathogenic bacteria from growing;
3. the invention utilizes lactobacillus plantarum to produce organic acid in the fermented traditional Chinese medicine, reduces the pH value of a fermentation substrate, inhibits the growth of mixed bacteria, and improves the flavor of the traditional Chinese medicine;
4. according to the invention, the Chinese medicinal composition fermented by bacillus subtilis and lactobacillus plantarum is used as an additive of the sow feed, so that the digestion and absorption of the sow on nutrient substances can be promoted, and the requirement of the sow in the lactation period on the nutrient substances can be ensured;
5. according to the invention, cellulase and pectase are added on the basis of microbial fermentation, and a high-activity enzyme preparation is added, so that the substrate after enzyme hydrolysis can be better utilized by thalli to form a forward promotion effect, the strain potential and substrate utilization rate are improved, and the activity of probiotic bacteria in fermented traditional Chinese medicines can be improved;
6. according to the invention, the addition ratio of bacillus subtilis and lactobacillus plantarum and the addition ratio of cellulase and pectase are adjusted, so that the drug effect of the traditional Chinese medicine prescription is highest;
7. the traditional Chinese medicine composition utilizing the synergistic fermentation of the bacterial enzymes has remarkable effects of promoting the growth and digestion of animal organisms and enhancing the immunity, improves the lactation capacity of lactating sows and the survival rate of piglets, and improves the cultivation benefit of pig farms.
Detailed Description
The present invention will be described in detail with reference to specific examples. The present embodiment is implemented on the premise of the technical scheme of the present invention, and a detailed implementation manner and a specific operation process are given, but the protection scope of the present invention is not limited to the following examples.
A traditional Chinese medicine composition for improving lactation yield of lactating sows is prepared from the following raw materials in parts by weight:
the traditional Chinese medicine comprises the following components: 6-8 parts;
bean pulp: 1-3 parts;
glucose: 1-3 parts;
bacillus subtilis: 1-3 parts;
lactobacillus plantarum: 8-10 parts;
cellulase: 3-5 parts;
pectase: 5-7 parts.
Further, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
chinese angelica root: 10-12 parts;
radix Scutellariae Baicalensis: 9-11 parts;
semen Vaccariae: 10-12 parts;
medulla Tetrapanacis: 4-6 parts;
fructus trichosanthis: 2-4 parts;
herba Lycopi: 3-5 parts.
The functions of the components in the traditional Chinese medicine are as follows:
the Chinese angelica and astragalus root have the functions of enhancing organism metabolism, strengthening heart, dilating blood vessels, promoting hematopoiesis of hemoglobin and enhancing organism immunity, and simultaneously have the functions of bidirectionally regulating uterus, and have the functions of anti-inflammatory, antibacterial and diuretic; the compatibility of the semen vaccariae, the medulla Tetrapanacis, the snakegourd fruit and the herba lycopi has the effects of stimulating menstrual flow and promoting lactation and increasing lactation capacity.
Further, the glucose is anhydrous glucose.
The invention also provides a preparation method of the traditional Chinese medicine composition for improving lactation yield of sows in lactation period, which comprises the following specific steps:
s1, crushing the traditional Chinese medicine components in the technical scheme, and mixing the crushed traditional Chinese medicine components with bean pulp and glucose to prepare a solid culture medium;
s2, adding bacillus subtilis and lactobacillus plantarum into the solid culture medium obtained in the step S1, and then fermenting to obtain a culture medium mixture;
s3, adding cellulase and pectase into the culture medium mixture obtained in the step S2, and then fermenting to obtain the traditional Chinese medicine composition.
In step S1, the traditional Chinese medicine components are crushed and then pass through a 80-100 mesh screen, and then are mixed with soybean meal and glucose to prepare a solid culture medium.
Further, in step S1, the traditional Chinese medicine components are added according to the weight ratio: bean pulp: anhydrous glucose=6 to 8:1 to 3:1 to 3.
Further, in step S2, lactobacillus plantarum is inoculated into an MRS liquid medium to obtain lactobacillus plantarum fermentation broth, bacillus subtilis is inoculated into an LB liquid medium to obtain bacillus subtilis fermentation broth, and lactobacillus plantarum fermentation broth and bacillus subtilis fermentation broth are inoculated into a solid medium.
The above further, the number of viable bacteria in the lactobacillus plantarum fermentation broth is not less than 1×10 8 The number of viable bacteria in the bacillus subtilis fermentation broth is not less than 1 multiplied by 10 8 。
The total inoculation amount of the lactobacillus plantarum fermentation liquor and the bacillus subtilis fermentation liquor is 10%.
The bacillus subtilis fermentation liquid is prepared by the following steps of: lactobacillus plantarum broth = 1-3: 8-10, preferably bacillus subtilis fermentation broth: lactobacillus plantarum broth = 1:9.
further, in step S2, the fermentation temperature is 37 ℃, and the fermentation time is 60-84 hours, preferably 72 hours.
Further, in step S3, the medium mixture is prepared by: cellulase: pectase=5 to 7:1 to 3:2 to 4.
Further, in the step S3, the enzyme activity of the cellulase is 72U/g, and the enzyme activity of the pectase is 108U/g.
Further, in step S3, the fermentation temperature is 37℃and the fermentation time is 60 to 84 hours, preferably 72 hours.
In addition, the invention also provides application of the traditional Chinese medicine composition for improving lactation yield of the sows in the lactation period, and the traditional Chinese medicine composition is added into the sow feed.
Further, the traditional Chinese medicine composition is prepared according to the following formula 1:1000 is added into sow feed for feeding.
The above embodiments are described in more detail below with reference to specific examples.
Example 1 preparation test
1.1 preparation of seed solutions of Lactobacillus plantarum and Bacillus subtilis
Taking out Lactobacillus plantarum preserved at 4deg.C in laboratory, inoculating Bacillus subtilis strain solution into newly prepared MRS and LB liquid culture medium respectively, placing Lactobacillus plantarum in 37 deg.C constant temperature incubator for static culture, and placing Bacillus subtilis strain in 100r/minCulturing at 37deg.C on a shaking table. Taking out after 24 hours, and counting the plate bacterial colony of lactobacillus plantarum and bacillus subtilis, wherein the number of viable bacteria is not less than 1 multiplied by 10 8 Then the seed liquid for fermentation is obtained and is put into a refrigerator with the temperature of 4 ℃ for cooling for standby.
1.2 screening of fermentation Strain in proper proportion
Inoculating the lactobacillus plantarum and bacillus subtilis seed solution into sterilized traditional Chinese medicine components separately and according to different proportions and total inoculation amount of 10 percent: bean pulp: anhydrous glucose at 7:2:1, carrying out single-strain fermentation and double-strain fermentation tests in different proportions, and carrying out fermentation grouping according to different proportions, wherein the fermentation groups are divided into a bacillus subtilis fermentation group, a lactobacillus plantarum fermentation group, bacillus subtilis and lactobacillus plantarum 1: group 9, bacillus subtilis and lactobacillus plantarum 2: group 8, bacillus subtilis and lactobacillus plantarum 3: group 7, bacillus subtilis and lactobacillus plantarum 4: group 6, bacillus subtilis and lactobacillus plantarum 5: group 5, bacillus subtilis and lactobacillus plantarum 6: group 4, bacillus subtilis and lactobacillus plantarum 7: group 3, bacillus subtilis and lactobacillus plantarum 8: group 2, bacillus subtilis and lactobacillus plantarum 9:1 group, 3 per minor group in parallel; mixed bacterial liquid of lactobacillus plantarum and bacillus subtilis of each group: the solid culture medium is added in a ratio of 1:1.5, after being uniformly mixed, the mixture is placed in a constant temperature incubator at 37 ℃ for standing fermentation for 72 hours, 50mL of the fermented traditional Chinese medicine product is taken into a centrifuge tube after the fermentation is finished, water bath at 80 ℃ is 30min, centrifugation is performed for 15min at 600 r/min, and part of supernatant is transferred to a 1.5mL centrifuge tube and is centrifuged for 2min at 12 000r/min. The content of crude polysaccharide in the supernatant was determined using phenol-sulfuric acid method. The crude polysaccharide assay results for each panel are shown in Table 1, B.subtilis and L.plantarum 1: the crude polysaccharide content of the 9 proportion group is 180.24mg/g highest.
TABLE 1 measurement of crude polysaccharide content for each group of different strains added in proportion
| Group of | Crude polysaccharide content (mg/g) |
| Bacillus subtilis fermentation group | 166.27±10.23 |
| Lactobacillus plantarum fermentation group | 165.38±9.35 |
| Bacillus subtilis and lactobacillus plantarum 1:9 groups | 180.24±10.34 |
| Bacillus subtilis and lactobacillus plantarum 2:8 groups of | 147.49±6.39 |
| Bacillus subtilis and lactobacillus plantarum 3:7 groups | 145.26±10.37 |
| Bacillus subtilis and lactobacillus plantarum 4:6 groups | 137.53±8.49 |
| Bacillus subtilis and lactobacillus plantarum 5: group 5 | 162.38±9.58 |
| Bacillus subtilis and lactobacillus plantarum 6: group 4 | 166.49±8.51 |
| Bacillus subtilis and lactobacillus plantarum 7: group 3 | 163.48±9.04 |
| Bacillus subtilis and plantsLactobacillus 8: group 2 | 169.39±8.29 |
| Bacillus subtilis and lactobacillus plantarum 9: group 1 | 166.49±9.20 |
1.3 screening of the appropriate ratio of fermenting enzymes
Inoculating the optimal strain proportion of 1.2 into sterilized solid fermentation substrates according to 10% of total inoculation amount, wherein the solid fermentation substrates are consistent with those of 1.2, and grouping according to different proportion combinations of added cellulase, pectase and two enzymes, wherein the group comprises a cellulase group, a pectase group, cellulase and pectase 1: group 9, cellulases and pectinases 2: group 8, cellulases and pectinases 3: group 7, cellulases and pectinases 4: group 6, cellulases and pectinases 5: group 5, cellulase and pectinase 6: group 4, cellulases and pectinases 7: group 3, cellulases and pectinases 8: group 2, cellulases and pectinases 9:1 group, 3 per minor group in parallel; each group is placed in a constant temperature incubator at 37 ℃ for standing fermentation for 72 hours after being uniformly mixed according to the feed liquid ratio of 1:1.5g/mL, 50mL of the fermented traditional Chinese medicine product is taken to a centrifuge tube after fermentation is finished, water bath at 80 ℃ is 30min, centrifugation is performed for 15min at 600 r/min, and part of supernatant is transferred to a centrifuge tube at 1.5mL and centrifuged for 2min at 12 000r/min. The content of crude polysaccharide in the supernatant was determined using phenol-sulfuric acid method. The crude polysaccharide assay results for each panel are shown in table 2, cellulase and pectinase 4: the crude polysaccharide content of the 6-proportion group was highest.
TABLE 2 measurement of crude polysaccharide content for each group with different enzyme addition ratios
| Group of | Crude polysaccharide content (mg-g) |
| Cellulase fermentation group | 221.17±16.03 |
| Pectase fermentation group | 225.42±19.25 |
| Cellulase and pectinase 1:9 groups | 221.54±17.74 |
| Cellulase and pectinase 2:8 groups of | 227.72±18.79 |
| Cellulase and pectinase 3:7 groups | 223.56±19.67 |
| Cellulase and pectinase 4:6 groups | 246.63±18.69 |
| Cellulase and pectinase 5: group 5 | 232.28±19.18 |
| Cellulase and pectinase 6: group 4 | 216.59±18.21 |
| Cellulase and pectinase 7: group 3 | 208.58±18.14 |
| Cellulase and pectinase 8: group 2 | 204.79±17.31 |
| Cellulase and pectinase 9: group 1 | 198.59±16.26 |
1.4 determination of total enzyme addition amount
Inoculating the optimal enzyme ratio measured in the above steps to a fermentation substrate according to the total addition amount of 30, 60, 90, 120, 150, 180 and 210U/g, grouping according to the total addition amount, and setting 3 parallel; feed ratio 1 for each parallel panel: 1.5g/mL, mixing, standing in a constant temperature incubator at 37deg.C for fermentation for 72h, sampling after fermentation, measuring crude polysaccharide content according to the above method, and measuring crude polysaccharide after total enzyme addition of each group, wherein the measurement results are shown in Table 3, and the ratio of cellulase to pectase is 4: the crude polysaccharide content was highest when the total amount of the addition was 180U/g.
TABLE 3 influence of total enzyme addition on crude polysaccharide
Example 2 application test
2.1 application test of optimum addition amount
The tests are divided into 3 groups, 180 sows delivered in the same batch are selected, and the tests are divided into 1 according to the adding proportion: 500. 1:1000:1:1500 groups of 60 heads each, divided into 3 parallel subgroups, each according to ratio 1: 500. 1:1000 and 1:1500, adding the synergistic fermentation product of the bacterial enzymes into the formula feed for feeding for 7 weeks, randomly selecting 6 sows during the test period to determine the production results, and mainly calculating the weight of weaned pigs, the average weight of weaned pigs, the daily weight gain of the sows, the daily average lactation yield of the sows and the survival rate of weaned pigs.
Daily average lactation (L/d) =average daily gain (g/d) of piglets x 4 x litter weaned pigs/1000;
weaned pig survival rate = weaned pig survival number per litter size x 100%.
The measured index data is subjected to statistical analysis by using SPSS22.0 software, and meanwhile, multiple comparison is carried out by combining with Duncan's method, the difference significance among treatments is checked, P <0.05 represents the difference significance, and P <0.01 represents the difference and significance; the test results are all expressed as mean ± standard deviation.
The measured production results of the sows are shown in Table 4, and from Table 4, it can be seen that the weaning nest weight, weaning average weight and daily gain of the lactating sows fed by the bacterial enzyme synergistic fermentation products of the 1:1000 group are all obviously higher than 1:500 and 1:1500 groups, 1:1000 groups of daily average lactation and weaned piglet survival rate higher than 1:1500 groups, but not up to the significance level (P > 0.05); in conclusion, the comparison shows that the synergistic fermentation product of the bacterial enzymes is added according to the proportion of 1:1000, and the effect of promoting the production performance of the sow is optimal.
TABLE 4 comparison of sow performance among groups
Note that: the acronyms are identical and indicate that there is no significant difference in comparison between groups (P > 0.05); the acronyms differ by a significant difference in comparison between the indicated groups (P < 0.05).
2.2 application of comparative experiments
The test is divided into 4 groups, 360 sows delivered in the same batch are selected, and the traditional Chinese medicine components of a control group, a traditional Chinese medicine group, a bacterial fermentation group and a bacterial enzyme synergistic fermentation group are consistent; each group has 90 heads, and is divided into 3 parallel groups, the control group sows are fed with formula feed, and the traditional Chinese medicine group is 1:1000, adding crushed traditional Chinese medicine powder into formula feed for feeding, wherein the fermentation product of 1.2 is prepared by the following steps of: 1000, and adding the fermented materials into formula feed for feeding, wherein the fermented materials of 1.3 are mixed according to the ratio of 1:1000 is added into the formula feed for feeding, the feeding time is 7 weeks, 6 sows are randomly selected during the test period to determine the production performance, and the method is mainly used for calculating the weight of weaned pigs, the average weight of weaned pigs, the daily gain, the daily average lactation yield of sows and the survival rate of weaned pigs.
Daily average lactation (L/d) =average daily gain (g/d) of piglets x 4 x litter weaned pigs/1000;
weaned pig survival rate = weaned pig survival number per litter size x 100%.
The measured index data is subjected to statistical analysis by using SPSS22.0 software, and meanwhile, multiple comparison is carried out by combining with Duncan's method, the difference significance among treatments is checked, P <0.05 represents the difference significance, and P <0.01 represents the difference and significance; the test results are all expressed as mean ± standard deviation.
The measured production results of the sows are shown in Table 5, and from Table 5, it can be seen that weaning litter weight, weaning average weight, daily gain, daily average lactation yield of the bacteria enzyme synergistic fermentation group and the bacteria fermentation group are all obviously higher than those of the control group (P < 0.05); the weaning litter weight, weaning average weight, daily gain, daily average lactation yield and weaned pig survival rate of the bacteria-enzyme synergistic fermentation group are obviously higher than those of the bacteria fermentation group (P < 0.05) and are obviously higher than those of the traditional Chinese medicine group (P < 0.01); the invention shows that the bacterial enzyme synergistic fermentation group can be widely popularized to improve the production performance of sows in the lactation period.
TABLE 5 comparison of sow performance among groups
Note that: the acronyms are identical and indicate that there is no significant difference in comparison between groups (P > 0.05); the shoulder letters are different and there is a significant difference in contrast between adjacent presentation groups (P < 0.05); the different and non-adjacent shoulder letters represent a very significant difference (P < 0.01).
EXAMPLE 3 comparative example for saving of traditional Chinese medicine resources
Group 3.1A: a traditional Chinese medicine composition for improving lactation yield of lactating sows is prepared from the following raw materials in parts by weight:
the traditional Chinese medicine comprises the following components: 6-8 parts;
bean pulp: 1-3 parts;
glucose: 1-3 parts;
bacillus subtilis: 1-3 parts;
lactobacillus plantarum: 8-10 parts;
cellulase: 3-5 parts;
pectase: 5-7 parts.
Further, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
chinese angelica root: 10-12 parts;
radix Scutellariae Baicalensis: 9-11 parts;
semen Vaccariae: 10-12 parts;
medulla Tetrapanacis: 4-6 parts;
fructus trichosanthis: 2-4 parts;
herba Lycopi: 3-5 parts.
The preparation method is the same as that of example 1
Group B: the traditional Chinese medicine composition for improving lactation yield of lactating sows is prepared from the following raw materials in parts by weight, wherein each of the traditional Chinese medicine components is added with 5 parts based on the traditional Chinese medicine component A:
chinese angelica root: 15-17 parts;
radix Scutellariae Baicalensis: 14-16 parts;
semen Vaccariae: 15-17 parts;
medulla Tetrapanacis: 9-11 parts;
fructus trichosanthis: 7-9 parts;
herba Lycopi: 8-10 parts.
Pulverizing the above Chinese medicinal materials, and sieving with 80-100 mesh sieve.
3.2 the finished products prepared in the A group and the B group are added into the formula feed according to the proportion of 1:1000 for feeding, and the test method is shown in the application test of the example 2.
TABLE 6 comparison of sow performance among groups
The measured production results of the sows are shown in Table 6, and from Table 6, the weaning nest weight, weaning average weight, daily gain, daily average lactation amount and weaned piglet survival rate of the sows in the lactation period of the group B are all obviously lower than those of the sows in the group A, but do not reach the significance level (P is more than 0.05); in conclusion, the comparison shows that the group B has no action of bacterial enzyme coordination on the basis of adding traditional Chinese medicine components, and the action effect of promoting the growth performance of the sow is obviously inferior to that of the group A, so that the Chinese herbal medicine resource can be saved under the action of bacterial enzyme coordination.
The previous description of the embodiments is provided to facilitate a person of ordinary skill in the art in order to make and use the present invention. It will be apparent to those skilled in the art that various modifications can be readily made to these embodiments and the generic principles described herein may be applied to other embodiments without the use of the inventive faculty. Therefore, the present invention is not limited to the above-described embodiments, and those skilled in the art, based on the present disclosure, should make improvements and modifications without departing from the scope of the present invention.
Claims (10)
1. The traditional Chinese medicine composition for improving lactation yield of lactating sows is characterized by being prepared from the following raw materials in parts by weight:
the traditional Chinese medicine comprises the following components: 6-8 parts;
bean pulp: 1-3 parts;
glucose: 1-3 parts;
bacillus subtilis: 1-3 parts;
lactobacillus plantarum: 8-10 parts;
cellulase: 3-5 parts;
pectase: 5-7 parts.
2. The traditional Chinese medicine composition for improving lactation yield of lactating sows as claimed in claim 1, wherein the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight:
chinese angelica root: 10-12 parts;
radix Scutellariae Baicalensis: 9-11 parts;
semen Vaccariae: 10-12 parts;
medulla Tetrapanacis: 4-6 parts;
fructus trichosanthis: 2-4 parts;
herba Lycopi: 3-5 parts.
3. The traditional Chinese medicine composition for improving lactation yield of lactating sows according to claim 1, wherein the glucose is anhydrous glucose.
4. A method for preparing a traditional Chinese medicine composition for improving lactation yield of lactating sows as claimed in any one of claims 1 to 3, which is characterized by comprising the following specific steps:
s1, crushing the traditional Chinese medicine components in claim 2, and mixing the crushed traditional Chinese medicine components with bean pulp and glucose to prepare a solid culture medium;
s2, adding bacillus subtilis and lactobacillus plantarum into the solid culture medium obtained in the step S1, and then fermenting to obtain a culture medium mixture;
s3, adding cellulase and pectase into the culture medium mixture obtained in the step S2, and then fermenting to obtain the traditional Chinese medicine composition.
5. The method for preparing a Chinese medicinal composition for increasing lactation yield of lactating sows as claimed in claim 4, wherein in the step S1, the Chinese medicinal components are crushed and then pass through a 80-100 mesh screen, and then are mixed with soybean meal and glucose to prepare a solid culture medium.
6. The method for preparing the traditional Chinese medicine composition for improving lactation yield of lactating sows as claimed in claim 4, wherein in the step S1, the traditional Chinese medicine composition comprises the following traditional Chinese medicine components in percentage by weight: bean pulp: anhydrous glucose=6 to 8:1 to 3:1 to 3.
7. The method for preparing a Chinese medicinal composition for increasing lactation yield of lactating sows as claimed in claim 4, wherein in step S2, lactobacillus plantarum is inoculated into MRS liquid medium to obtain Lactobacillus plantarum fermentation broth, bacillus subtilis is inoculated into LB liquid medium to obtain Bacillus subtilis fermentation broth, lactobacillus plantarum fermentation broth and Bacillus subtilis fermentation broth are inoculated into solid medium,
the viable count of the lactobacillus plantarum fermentation liquor is not less than 1 multiplied by 10 8 The number of viable bacteria in the bacillus subtilis fermentation broth is not less than 1 multiplied by 10 8 ；
The total inoculation amount of the lactobacillus plantarum fermentation liquor and the bacillus subtilis fermentation liquor is 10%;
according to the weight ratio of the addition, the bacillus subtilis fermentation liquid: lactobacillus plantarum broth = 1-3: 8-10;
the fermentation temperature is 37 ℃ and the fermentation time is 60-84 h.
8. The method for preparing a traditional Chinese medicine composition for improving lactation yield of lactating sows as claimed in claim 4, wherein in the step S3, the culture medium mixture is prepared by the following steps: cellulase: pectase=5 to 7:1 to 3:2 to 4;
further, in the step S3, the enzyme activity of the cellulase is 72U/g, and the enzyme activity of the pectase is 108U/g;
further, in the step S3, the fermentation temperature is 37 ℃ and the fermentation time is 60-84 hours.
9. Use of a traditional Chinese medicine composition for increasing lactation yield of lactating sows, characterized in that the traditional Chinese medicine composition according to any one of claims 1-3 is added into sow feed.
10. The use of a traditional Chinese medicine composition for increasing lactation yield of lactating sows according to claim 9, wherein the traditional Chinese medicine composition is prepared according to the following formula 1:1000 is added into sow feed for feeding.
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