CN107625803A - A kind of Chinese medicine preparation preparation method and Traditional Chinese medicine extraction method for improving animal lactation - Google Patents
A kind of Chinese medicine preparation preparation method and Traditional Chinese medicine extraction method for improving animal lactation Download PDFInfo
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- CN107625803A CN107625803A CN201710977232.6A CN201710977232A CN107625803A CN 107625803 A CN107625803 A CN 107625803A CN 201710977232 A CN201710977232 A CN 201710977232A CN 107625803 A CN107625803 A CN 107625803A
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- bacillus subtilis
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Abstract
The invention provides a kind of Chinese medicine preparation, wherein, the Chinese medicine preparation includes the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, the stem pith of the rice-paper plant, Ligusticum wallichii, the bighead atractylodes rhizome, teasel root, akebi, radix glycyrrhizae, the seed of cowherb, Chinese medicine residue and bacillus subtilis, the content of bacillus subtilis in the formulation after the Chinese medical extract to play both sides of the street and extraction are 108 109/g.The effect sum that Clinical practice effect of the present invention is used alone than the positive effect of traditional Chinese medicine powder and more than Chinese medicine with bacillus subtilis, makes 1+1 be more than 2.Present invention also offers a kind of traditional Chinese medicine extraction technology, utilizes the purity of the raising Chinese medical extract such as caused cellulase, amylase in bacillus subtilis growth course.
Description
Technical field
The invention belongs to biomedicine field, and in particular to a kind of Chinese medicine preparation preparation method for improving animal lactation is with
Medicine extracting method.
Background technology
Chinese medicine is the rarity in China.Traditional boiling, powder processed are progressively by the extraction of hot refluxed cryogenic, dynamic vacuum extraction etc.
Process substitutes.Decocting method is still the core procedure of traditional Chinese medicine extraction in actual production, and the effective of Chinese medicine is removed in decoction process
Inevitably the impurity such as starch, dextrin, colloid is extracted in the lump outside composition, the more fibre-bearings of Chinese medicine are in extraction process
The middle speed for influenceing extraction, the factor of the above two are to restrict two big main causes of medicine extraction cost.
Republic of China Veterinary Pharmacopoeia version in 2010 two (lactogenesis dissipates) discloses the scattered preparation technology of lactogenesis：The Radix Astragali
30g Radix Codonopsis 30g, Radix Angelicae Sinensis 45g, stem pith of the rice-paper plant 15g, Ligusticum wallichii 15g, bighead atractylodes rhizome 30g, teasel root 25g, akebi 15g, radix glycyrrhizae 15g, the seed of cowherb
30g, play both sides of the street 25g.The taste of the above 11 crushes, and sieves, and mixes, produces.But the product bioavilability of the technique productions is low
Shortcoming.
The food-safety problem such as antibiotic residue is more paid attention to by broad masses with the improvement of people ' s living standards.In
Medicine from animals and plants in the complete drug residue free of machine conversion in the body, microorganism alternatively one of scheme of antibiotic with work
The practicable also depth ripe, safe to use of skill is big by the welcome probiotics of the people, prebiotics, Synbiotics theory and practice
Quantity research.Chinese medicine and the case that beneficial bacterium is used in combination can be found everywhere in Animal husbandry production.
Bacillus subtilis consumes rapidly the free oxygen in enteron aisle, causes enteron aisle hypoxemia, promotes beneficial to anaerobism bacteria growing,
Connect and suppress other pathogenic bacteria growings.Stimulating animal immune organ grows, and activation T, bone-marrow-derived lymphocyte, improves immune globulin
White and antibody level, strengthen cellular immunity humoral immune function, improve herd immunity.Bacillus subtilis thalline itself synthesizes
The enzymes such as protease, lipase, cellulase, work is together played with the digestive enzymes in animal body (human body) in alimentary canal
With.A variety of B family vitamins such as vitamin B1, B2, B6, nicotinic acid can be synthesized, improve the work of interferon and macrophage in animal body
Property.
Therefore solve the impurity in extraction of traditional Chinese medicine using modern science and technology and unwanted contributions are converted into useful component, simultaneously
Addition probiotics solves the problems, such as antibiotic residue.The present invention utilizes caused cellulose in bacillus subtilis regrowth process
The macromolecular substances such as decomposition of cellulose, starch, protein in extraction process make it be converted into animal by Chinese medicine for enzyme, amylase
Small-molecule substance can be absorbed.
The content of the invention
The first aspect of the present invention is to provide a kind of Traditional Chinese drug microecology combination that can improve animal lactation, wherein described
Combined traditional Chinese medicine include the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, the stem pith of the rice-paper plant, Ligusticum wallichii, the bighead atractylodes rhizome, teasel root, wooden barrel, radix glycyrrhizae, the seed of cowherb, play both sides of the street；It is micro-
It is bacillus subtilis described in ecology.
To achieve the above object, the technical scheme that the present invention takes is as follows：
Traditional Chinese drug microecology compositions, process of the present invention is divided into following steps：
1. bacillus subtilis is enlarged into culture, using fluid nutrient medium culture, 35-37 degrees Celsius of condition of culture,
Hour time 24-36, determine and produced per ml viable counts for 108-109.
2. Chinese medicine is beaten into coarse powder, add 8 times of amount water immersions, add fermentation of bacillus subtilis.Fermentation condition is taken the photograph for 30-37
Family name's degree, fermentation time 24 hours, rotating speed 60-120r/ minutes.The purpose of this step, is utilized in bacillus subtilis regrowth process
Caused cellulase is decomposed the fiber of Chinese medicine.
3. traditional Chinese medicine extyaction, low temperature circumfluence distillation 2 hours, 70 degree of temperature.The purpose of this step is to greatest extent
Extracts active ingredients in Chinese medicine are come out.
4. after fermentation, fermentation condition is 30-37 degree, fermentation time 24 hours, rotating speed 60-120r/ minutes.This step purpose
It is to utilize caused amylase etc. in bacillus subtilis regrowth process to divide the macromoleculars such as the starch after traditional Chinese medicine extraction, colloid
Solution into be easy to animal absorption small molecule.
5. concentration, 70 degree of concentrations make concentrate relative density reach 1.15.
6. drying, 65 degree of concentrate after fermentation, which is dried under reduced pressure, makes moisture less than 8.0%, while by after extraction
Medicine residue is dried.
7. be pulverized and mixed, dried extract with Chinese medicine residue be crushed into 40 mesh sieves respectively it is well mixed and produce.Survey
Fixed every g viable counts containing bacillus subtilis are in 108-109.
The second aspect of the invention, which also resides in, provides a kind of method of traditional Chinese medicine extraction.Purpose is to improve Chinese medical extract to have
Imitate the yield and purity of composition.
To achieve the above object, the technical scheme that the present invention takes is as follows：
1. bacillus subtilis is enlarged into culture, using fluid nutrient medium culture, 35-37 degrees Celsius of condition of culture,
Hour time 24-36, determine and produced per ml viable counts for 108-109.
2. Chinese medicine is beaten into coarse powder, add 6-8 times to measure water immersion, add fermentation of bacillus subtilis.Fermentation condition is 30-37
Degree Celsius, fermentation time 24 hours, rotating speed 60-120r/ minutes.The purpose of this step is to utilize bacillus subtilis regrowth process
In caused cellulase the fiber of Chinese medicine is decomposed.
3. traditional Chinese medicine extyaction, being extracted according to the property of different medicinal materials, require to carry out alcohol precipitation etc. according to different
Step.
4. after fermentation, fermentation condition is 30-37 degree, fermentation time 24 hours, rotating speed 60-120r/ minutes.This step purpose
It is to utilize caused amylase etc. in bacillus subtilis regrowth process to divide the macromoleculars such as the starch after traditional Chinese medicine extraction, colloid
Solution into be easy to animal absorption small molecule.So as to improve the purity of extract.
Embodiment
The invention will now be further described with reference to specific embodiments, and advantages of the present invention and feature will be with description more
To be clear.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.Those skilled in the art
It should be understood that the details and form of technical solution of the present invention can be carried out without departing from the spirit and scope of the invention
Modifications or substitutions, but these modifications and replacement are each fallen within protection scope of the present invention.
Embodiment 1,
Prepare 1, take bacillus subtilis to be inoculated into 35-37 degrees Celsius of condition of culture on full liquid culture medium, time 24-36
Hour, it is that 108-109 is standby to determine per ml viable counts.
Take Radix Astragali 30g Radix Codonopsis 30g, Radix Angelicae Sinensis 45g, stem pith of the rice-paper plant 15g, Ligusticum wallichii 15g, bighead atractylodes rhizome 30g, teasel root 25g, akebi 15g, radix glycyrrhizae
15g, seed of cowherb 30g, the taste coarse powder of more than 25g 11 that plays both sides of the street add 8 times of amount water to add bacterium solution 200ml after soaking one hour, opened
Agitating device makes rotating speed control in 60-120r/ minutes.Fluid temperature is controlled between 30-37 degrees Celsius, is incubated 24-36 hours.
Open extraction system state modulator：70 degree, vacuum -0.06-0.08Mpa of Extracting temperature, refluxing extraction 2 hours.Filtration, filtrate
Let cool and add bacterium solution 100ml, opening agitating device makes rotating speed control be taken the photograph in 60-120r/ minutes, fluid temperature control in 30-37
Between family name's degree, 24 hours are incubated.Concentrated at 70 degree, be concentrated into relative density and surveyed for 1.15-1.20 heat.Concentrate 65 is subtracted
Press dry it is dry make moisture drop to less than 8.0% to crushed 60 mesh sieves, while the Chinese medicine residue dries pulverizing after extraction is crossed into 40 mesh
Sieve and the material after above-mentioned crushing is sufficiently mixed uniform aluminium foil bag dispenses and produce.Measure exists per g viable counts containing bacillus subtilis
108-109。
Prepare 2, take bacillus subtilis to be inoculated into 35-37 degrees Celsius of condition of culture on full liquid culture medium, time 24-36
Hour, it is that 108-109 is standby to determine per ml viable counts.Extracting liquorice 1000g coarse powder adds 8 times of amount water to add bacterium solution after soaking 1 hour
200ml, opening agitating device makes rotating speed control in 60-120r/ minutes.Fluid temperature is controlled between 30-37 degrees Celsius, insulation
24-36 hours.Open extraction system state modulator：70 degree, vacuum -0.06-0.08Mpa of Extracting temperature, refluxing extraction 5 is small
When, standing overnight makes precipitation, takes supernatant to add bacterium solution 100ml, and opening agitating device makes rotating speed control in 60-120r/ minutes,
Fluid temperature is controlled between 30-37 degrees Celsius, is incubated 24 hours.Concentrated at 100 degree, be concentrated into thick paste.By concentrate
65 are dried under reduced pressure and make moisture drop to less than 6.0% to crushed 80 mesh sieves, dispensed and produced with aluminium foil bag.
Embodiment 2, the inventive method are compared with the preparation acquired by other preparation methods
Sample 1, prepare 1 preparation gained sample.
Sample 2, prepare 2 preparation gained samples.
Sample 3, Chinese veterinary pharmacopoeia：Radix Astragali 30g Radix Codonopsis 30g, Radix Angelicae Sinensis 45g, stem pith of the rice-paper plant 15g, Ligusticum wallichii 15g, bighead atractylodes rhizome 30g, teasel root
25g, akebi 15g, radix glycyrrhizae 15g, seed of cowherb 30g, the 25g that plays both sides of the street crushed 40 mesh sieves, be well mixed, and aluminium foil bag packing is
.
Sample 4, extracting liquorice 1000g coarse powder add 8 times of amount water to soak 1 hour：Extracting temperature 80-90 degree, vacuum -0.06-
0.08Mpa, refluxing extraction 5 hours, standing overnight makes precipitation, takes supernatant.Concentrated at 100 degree, be concentrated into thick paste.Will be dense
Contracting liquid 65, which is dried under reduced pressure, to be made moisture drop to less than 6.0% to crushed 80 mesh sieves, dispensed and produced with aluminium foil bag.
Sample 5, bacillus subtilis is taken to be inoculated into 35-37 degrees Celsius of condition of culture on full liquid culture medium, time 24-36
Hour, determine and produced per ml viable counts for 108-109.
Each sample contrast experiment in embodiment 3, the embodiment of the present invention 2.
Experiment 1, milk production of cow contrast experiment
Yanshi City's raising dairy cattle cell chooses healthy 40 points 4 groups, every group 10 of Holstein-Friesian.Control group is according to raiser
Daily raising custom feeding, test the 1 group of feeding 200g of sample 3 per cow head, 2 groups of experiment daily and feed sample per cow head daily
The 200g of product 1,3 groups of experiment feed the 200ml of sample 5 per cow head daily.Experiment start first 5 days 3 groups according to the daily feeding of raiser
Foster custom does not add trial target and records every group of daily average milk production as basic data.Record feeding 10-15 days, 25-30 days,
40-45 days, 55-60 day datas are compared.
Table 1
Conclusion：The output of milk significantly improves after comparing proof 1 group of experiment feeding 15 days by table 1, the output of milk after feeding 30 days
It is stable to improve the 3.39kg/ previous day.Compare one group of increase output of milk 2.0kg/ previous day of experiment after testing 2 groups of Long-term Feedings.Test 4 groups of length
Increase the previous day of the output of milk 0.91/ than control group after phase feeding.The output of milk significantly improves after testing 2 groups of feedings 15 days, feeds 30 days
The output of milk exceedes 1 group of experiment afterwards, and the output of milk stably improves the 5.34kg/ previous day after feeding 45 days, more than 1 group of experiment and 3 groups of sums.
It is 4.4 yuan/kg that raw milk, which stores up price this area, and the market price of 200g samples 3 is 8 yuan, is given milk after Long-term Feeding per cow head
Amount increase value is 3.39*4.4-8=5.56 members, and the market price of 200g samples 1 is 11 yuan, is given milk after Long-term Feeding per cow head
Amount increase value is 5.34*4.4-11=12.5 members.
Increased both production and value using the product of the present invention on breeding milk cow substantially.
Experiment 2, piglet weightening contrast experiment
Choose 40 points 3 groups, every group 10 of farrowing sow in Mengjin County pig farm.Control group is accustomed to according to the daily raising of raiser
Feeding, 1 component puerperium of experiment daily every sow feeding sample 360g, 2 component puerperiums of experiment daily every sow feeding sample 1
60g, the 3 component puerperiums of experiment, every 60ml of sow feeding sample 5 was until 28 days weaned piglets daily.Record piglet average weight gain
With mortality analysis.
Table 2
Conclusion：Compare the sow after proving the feeding present invention by table 2, piglet weightening dissipates than traditional lactogenesis to be significantly improved,
Survival rate significantly improves and 2 groups of experiment of increasing weight is more than 1 group of experiment and 3 groups of sums.
Experiment 3, licorice predominant amount of the present invention is not used with using licorice predominant amount of the present invention
Contrast.3 batches of continuous extraction.
Table 3
Conclusion：Being compared by table 3 proves substantially to increase using the licorice glycyrrhizic acid content of the present invention.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (7)
1. a kind of Chinese medicine preparation, wherein, the Chinese medicine preparation includes the Radix Astragali, Radix Codonopsis, Radix Angelicae Sinensis, the stem pith of the rice-paper plant, Ligusticum wallichii, the bighead atractylodes rhizome, teasel root, wood
Lead to, radix glycyrrhizae, the seed of cowherb, residue and bacillus subtilis after the Chinese medical extract to play both sides of the street and extraction, described withered grass bud
The content of spore bacillus in the formulation is 108-109/g.
2. Chinese medicine preparation according to claim 1, wherein, preparation is in preparation process, addition bacillus subtilis before extracting
The processing procedure that bacterium is carried out.
3. Chinese medicine preparation according to claim 1, wherein, preparation adds bacillus subtilis in preparation process after extraction
The processing procedure that bacterium is carried out.
4. the Chinese medicine preparation according to claim any one of 1-3 is in the medicine for improving animal lactation amount, additive is prepared
Application.
5. a kind of Traditional Chinese medicine extraction method, wherein, the processing method of addition bacillus subtilis in described method.
Addition bacillus subtilis is carried out before 6. the method according to claim 11 is extracted during Chinese medical extract is prepared
Processing procedure.
7. the method according to claim 11 is added bacillus subtilis after being extracted during preparing Chinese medical extract and carried out
Processing procedure.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116898896A (en) * | 2023-08-30 | 2023-10-20 | 福建益昕葆生物制药有限公司 | Traditional Chinese medicine composition for improving lactation yield of lactating sows and preparation and application thereof |
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| CN101164429A (en) * | 2006-10-19 | 2008-04-23 | 天津市润拓生物技术有限公司 | Method for preparing traditional Chinese herb feed additive and its glagctogogue powder preparation |
| CN103110021A (en) * | 2013-03-08 | 2013-05-22 | 湖南农业大学 | Chinese herbal medicine premix with significant lactation prompting function to lactating sow and feed containing premix |
| CN106913670A (en) * | 2017-04-27 | 2017-07-04 | 西南大学 | Growth promotion and strong immune traditional Chinese medicine for livestock biological agent and preparation method thereof |
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Patent Citations (3)
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| CN101164429A (en) * | 2006-10-19 | 2008-04-23 | 天津市润拓生物技术有限公司 | Method for preparing traditional Chinese herb feed additive and its glagctogogue powder preparation |
| CN103110021A (en) * | 2013-03-08 | 2013-05-22 | 湖南农业大学 | Chinese herbal medicine premix with significant lactation prompting function to lactating sow and feed containing premix |
| CN106913670A (en) * | 2017-04-27 | 2017-07-04 | 西南大学 | Growth promotion and strong immune traditional Chinese medicine for livestock biological agent and preparation method thereof |
Non-Patent Citations (1)
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116898896A (en) * | 2023-08-30 | 2023-10-20 | 福建益昕葆生物制药有限公司 | Traditional Chinese medicine composition for improving lactation yield of lactating sows and preparation and application thereof |
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Application publication date: 20180126 |