CN116139257A

CN116139257A - Traditional Chinese medicine formula for treating postpartum hypogalactia of sow and preparation method thereof

Info

Publication number: CN116139257A
Application number: CN202211720621.8A
Authority: CN
Inventors: 覃智斌; 邓红玉; 罗作明; 张志榕; 梁世仁; 吴有林
Original assignee: Fujian Yixinbao Biopharmaceutical Co ltd
Current assignee: Fujian Yixinbao Biopharmaceutical Co ltd
Priority date: 2022-12-30
Filing date: 2022-12-30
Publication date: 2023-05-23

Abstract

The invention discloses a traditional Chinese medicine formula for treating postpartum hypogalactia of sows and a preparation method thereof, wherein active substances in the traditional Chinese medicine formula comprise the following components: stone bell milk, saltpeter, white stone, medulla Tetrapanacis, platycodon grandiflorum, motherwort, gypsum, rhizoma anemarrhenae, prepared rheum officinale, sodium bicarbonate, liquorice, composite probiotics and a composite enzyme preparation. Compared with the prior art, the traditional Chinese medicine formula prepared by the invention adopts a plurality of traditional Chinese medicines to divide into two doses, respectively solves the problems of postpartum hypogalactia and subsequent difficult defecation of sows, and has the effects of resisting bacteria and inflammation, resisting viruses, improving the immunity of organisms, regulating intestinal flora and promoting intestinal digestion and absorption.

Description

Traditional Chinese medicine formula for treating postpartum hypogalactia of sow and preparation method thereof

Technical Field

The invention relates to the technical field of livestock feed additives, in particular to a traditional Chinese medicine formula for treating postpartum hypogalactia of sows and a preparation method thereof.

Background

The breast of sow has great difference with other livestock such as cattle and sheep. The breast of the cow and the sheep is provided with a breast storage pool, the breast storage pool of the pig is extremely undeveloped, milk cannot be stored, and the piglet cannot suck the milk at any time. Milk can only be consumed when the sow is "put down". The initiation of this history requires adequate stimulation of the piglets and an increase in mammary pressure, and stress on the sow by the external environment can cause the release of the body antagonistic oxytocin, resulting in a weaning arrest.

The sow is 60d in lactation period, the lactation amount is about 450kg, daily average lactation is 7.5kg, the water content ratio in the emulsion is above 70%, the sow can enter a feedback regulation stage when water loss is excessive, the second half of intestinal tract can strengthen water reabsorption, dry stool and difficult defecation are caused, and the feed intake of the sow is influenced, so that the continuous lactation of the sow is influenced. The lactation promoting agent used in the market at present only has a certain treatment effect on the postpartum hypogalactia of sows, and has no related treatment on postpartum sow constipation or constipation which are easy to cause later. Therefore, it is important to improve the original emulsion feed additive, expand the indication and promote the digestion and absorption of sow.

The invention patent CN104306472A discloses a traditional Chinese medicine preparation for preventing and treating postpartum hypogalactia of sows and a preparation method thereof, wherein the traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 5 to 15 parts of roasted astragalus root, 8 to 13 parts of glossy privet fruit, 6 to 15 parts of cowherb seed, 3 to 10 parts of herba patriniae, 4 to 12 parts of radix scutellariae, 10 to 30 parts of teasel root, 4 to 12 parts of epimedium, 5 to 15 parts of magnolia officinalis, 7 to 15 parts of dandelion, 5 to 13 parts of red paeony root and 2 to 7 parts of angelica. The traditional Chinese medicine oral powder and injection for preventing and treating the postpartum hypogalactia of the sow are prepared from the raw material components, are safe and reliable, have no side effect and quick response, have a preventive and therapeutic effect on the sow, can effectively prevent and treat symptoms such as mastitis, endometritis, cold, puerperal fever and the like, can effectively promote lactation yield, and can improve lactation yield by 32%, so that the growth and development level of piglets is improved, and the net weight of the piglets is increased by 10%. However, the traditional Chinese medicine preparation prepared by the invention can not effectively treat constipation, and has low medicine absorption and poor effect.

Disclosure of Invention

In view of the defects that the traditional Chinese medicine formula in the prior art is poor in absorption effect, incapable of treating constipation and low in drug effect, the invention aims to provide the traditional Chinese medicine formula for treating postpartum hypogalactia of sows, which is good in absorption effect and obvious in effect, and the preparation method thereof.

In order to achieve the above object, the present invention adopts the following technical scheme:

a traditional Chinese medicine formula for treating postpartum hypogalactia of sows comprises the following preparation methods in parts by weight:

adding 1-3 parts of active ingredients into 8-12 parts of water, magnetically stirring for 20-50 min at a stirring speed of 400-800 rpm to obtain a liquid medicine, spraying the liquid medicine on the surface of 0.5-2 parts of modified powder in a fluidized bed, and then drying at 40-70 ℃ for 10-20 min to obtain a medicine carrier; the operation parameters of the fluidized bed are set to be the spraying speed of 2-5 g/min, the atomization air pressure of 1.0-3.0 bar, the material temperature of 30-40 ℃, the inlet temperature of 40-70 ℃ and the spraying air velocity of 10-30 m ³ /h；

Adding 1-3 parts of auxiliary agent into 8-12 parts of water, adding 0.4-0.6 part of lactose and 0.8-1.2 parts of tapioca starch, stirring for 20-40 min at a stirring speed of 100-400 rpm to prepare slow-release coating liquid, spraying the slow-release coating liquid on the surface of the medicine carrier prepared in the step T1 by a fluidized bed, wherein the operation parameters of the fluidized bed are set to be that the spraying speed is 1-3 g/min, the atomization air pressure is 1.0-3.0 bar, the material temperature is 25-35 ℃, the inlet temperature is 30-50 ℃, and the air spraying speed is 10-20 m ³ And/h, drying at 30-50 ℃ for 20-30 h to obtain the traditional Chinese medicine formula.

The active ingredients comprise the following raw materials: stone bell milk, saltpeter, white stone, medulla Tetrapanacis, platycodon grandiflorum, motherwort, gypsum, rhizoma anemarrhenae, prepared rheum officinale, sodium bicarbonate, liquorice, composite probiotics and a composite enzyme preparation.

The stone bell milk has the treatment effects of hypogalactia, lower-jiao exhaustion and yin strengthening, and is supplemented with Sal Nitri, white stone, medulla Tetrapanacis, radix Platycodi and Glycyrrhrizae radix, so as to promote lactation of lean sow; by feeding gypsum, rhizoma anemarrhenae, prepared rheum officinale, sodium bicarbonate, liquorice and compound enzyme preparation, the drug effect of the traditional Chinese medicine can be improved, the problem of the subsequent intestinal tract can be treated by probiotics, and stable production of a pig farm delivery room is ensured.

Preferably, the active ingredients comprise the following raw materials in parts by weight: 10-30 parts of stone clock emulsion, 10-30 parts of saltpeter, 10-30 parts of white stone, 20-90 parts of medulla Tetrapanacis, 40-80 parts of platycodon grandiflorum, 30-80 parts of motherwort, 30-60 parts of gypsum, 30-60 parts of rhizoma anemarrhenae, 30-80 parts of prepared rheum officinale, 10-50 parts of sodium bicarbonate, 10-20 parts of liquorice, 3-5 parts of compound probiotics and 1-2 parts of compound enzyme preparation.

The preparation method of the active ingredient comprises the following steps:

step 1, weighing the stalactite, the niter, the white stone, the medulla Tetrapanacis, the platycodon grandiflorum and the motherwort according to the weight parts; grinding and cooling to room temperature to obtain a traditional Chinese medicine mixture A;

step 2, weighing gypsum, rhizoma anemarrhenae, prepared rhubarb, sodium bicarbonate and liquorice according to parts by weight, grinding and cooling to room temperature to obtain a traditional Chinese medicine mixture B;

and 3, mixing the traditional Chinese medicine mixture A prepared in the step 1 with the traditional Chinese medicine mixture B prepared in the step 2, grinding and sieving to obtain traditional Chinese medicine mixed powder, adding probiotic powder, adding compound enzyme preparation powder, stirring, and drying to obtain active ingredients.

Preferably, the preparation method of the active ingredient comprises the following steps:

step 1, weighing the stalactite, the niter, the white stone, the medulla Tetrapanacis, the platycodon grandiflorum and the motherwort according to the weight parts; placing the mixture into a pulverizer, grinding the mixture for 1 to 3 hours, and cooling the mixture to room temperature to obtain a traditional Chinese medicine mixture A;

step 2, weighing gypsum, rhizoma anemarrhenae, prepared rhubarb, sodium bicarbonate and liquorice according to parts by weight, putting into a pulverizer, grinding for 1-3 hours, and cooling to room temperature to obtain a traditional Chinese medicine mixture B;

and 3, mixing the traditional Chinese medicine mixture A prepared in the step 1 with the traditional Chinese medicine mixture B prepared in the step 2, grinding for 2-5 hours, sieving with a 60-100-mesh sieve to obtain traditional Chinese medicine mixed powder, adding probiotic powder, adding compound enzyme preparation powder, stirring for 20-40 min at 50-200 rpm, drying at 30-50 ℃, and controlling the water content to be 5-10 wt% to obtain the active ingredient.

The probiotic powder is freeze-dried powder and consists of the following strains in parts by weight: 1 to 2 parts of lactobacillus plantarum, 2 to 3 parts of bacillus subtilis, 1 to 2 parts of saccharomyces cerevisiae, 1 to 2 parts of enterococcus faecalis and 1 to 2 parts of bacillus coagulans.

The composite enzyme preparation powder is dry powder and consists of the following enzyme preparations in parts by weight: 3-5 parts of cellulase, 2-3 parts of xylanase and 2-3 parts of glucose oxidase.

The preparation method of the auxiliary agent comprises the following steps:

s1, crushing shrimp and crab shells, sieving, adding into a sodium hydroxide aqueous solution, stirring, adding absolute ethyl alcohol, stirring, filtering, collecting solids, cleaning and drying to obtain a pretreated substance;

s2, adding the pretreated matter prepared in the step S1 into a sodium hydroxide aqueous solution, stirring, cooling by adopting an ice water bath, sieving, collecting solids, and flushing to obtain an extract;

s3, adding L-histidine into water to obtain an L-histidine water solution, adding sodium alginate powder into water, and stirring to obtain a homogeneous solution; adding the extract prepared in the step S2 into the homogeneous solution, then adding glycerol, stirring, adding L-histidine aqueous solution, stirring again,

evaporating and then placing in an incubator for preservation to obtain the auxiliary agent.

Preferably, the preparation method of the auxiliary agent comprises the following steps in parts by weight:

s1, crushing 3-5 parts of shrimp and crab shells into fine particles by using a superfine crusher, sieving by a 100-300 mesh sieve, adding into 15-25 parts of 2-6wt% sodium hydroxide aqueous solution, stirring for 3-8 hours at 60-70 ℃, stirring at 100-300 rpm, adding 4-6 parts of absolute ethyl alcohol, stirring for 4-8 hours at room temperature, stirring at 200-600 rpm, filtering by a 300-500 mesh sieve, collecting solids, washing with water until washing water is neutral, and drying for 10-20 hours at 40-70 ℃ in a vacuum oven to obtain a pretreated substance;

s2, adding 0.5 to 1.5 parts of the pretreated matter prepared in the step S1 into 2 to 3 parts of 30 to 35 weight percent sodium hydroxide aqueous solution, stirring for 1 to 5 hours at the temperature of 70 to 110 ℃ at the stirring speed of 100 to 300rpm, cooling to room temperature, cooling by using ice water bath, sieving by a 300 to 500-mesh sieve, collecting solids, and washing with water for 1 to 3 times to obtain an extract;

s3, adding 0.4-0.8 part of L-histidine into 2-5 parts of water to obtain an L-histidine water solution, adding 0.4-0.8 part of sodium alginate powder into 4-6 parts of water at 20-30 ℃, and stirring for 1-3 hours to obtain a homogeneous solution; adding 0.1-0.5 part of the extract prepared in the step S2 into a homogeneous solution, adding 0.04-0.08 part of glycerol, stirring for 1-3 hours at 300-600 rpm, adding an L-histidine aqueous solution, stirring for 10-30 minutes at a stirring speed of 100-300 rpm, evaporating at 40-60 ℃ for 60-80 hours, then placing in an incubator at a temperature of 20-30 ℃ and a relative humidity of 45-55%, and preserving for 30-50 hours to obtain the auxiliary agent.

The preparation method of the modified powder comprises the following steps:

z1, adding konjak mannan into water, stirring, adjusting the pH with a sodium hydroxide aqueous solution, adding hydrogen peroxide, adding a sodium bisulfate aqueous solution to terminate the reaction, adjusting the pH, precipitating with absolute ethyl alcohol, filtering, cleaning, drying, grinding and sieving to obtain pretreated powder;

and Z2, adding the pretreated powder prepared in the step Z1 into water, slowly adding tapioca starch, stirring, drying, grinding, sieving, and heating to obtain the modified powder.

Preferably, the preparation method of the modified powder comprises the following steps of:

z1, adding 1.5 to 2 parts of konjak mannan into 8 to 12 parts of water, stirring for 20 to 40 minutes at 400 to 600rpm and 40 to 50 ℃, adjusting the pH to 7.5 to 8.5 by using 0.5 to 2 weight percent sodium hydroxide aqueous solution, adding 0.2 to 0.5 part of hydrogen peroxide every 5 to 15 minutes, adding 2 to 5 times, adding 0.5 to 2mol/L sodium bisulfate aqueous solution after 3 to 5 hours to adjust the pH to 6 to 7, precipitating by using absolute ethyl alcohol, filtering by a vacuum filter, washing for 2 to 5 times by using 65 to 80 weight percent ethanol aqueous solution, vacuum drying for 1 to 3 hours at 40 to 60 ℃, and grinding and sieving by using a 60 to 150-mesh sieve to obtain pretreated powder;

and Z2, adding 0.5-2 parts of the pretreated powder prepared in the step Z1 into 3-5 parts of water, slowly adding 0.1-0.3 part of tapioca starch, stirring for 0.5-2 hours at room temperature, wherein the stirring speed is 100-300 rpm, drying at 30-50 ℃ until the water content is 6-12 wt%, further grinding, sieving through a 60-200 mesh sieve, and heating for 2-6 hours at 110-140 ℃ to obtain the modified powder.

Stone bell milk, stalactite, sweet taste, warm nature, no toxicity; enters the spleen of foot taiyin, lung of hand taiyin, kidney of foot shaoyin and liver meridian of foot jueyin. The main functions are as follows: five-strain seven-wound cough with adverse qi upward, cold cough, voice, five viscera and hundred diseases, nine-theft, lactation, qi-tonifying and deficiency-tonifying, weak pain and cold, exhaustion of lower jiao and yin-strengthening.

Niter, nitum, also known as mirabilite, kalium, etc.; bitter, cold and nontoxic. The main functions are as follows: the accumulation of heat in the five zang organs and the stomach distention and closure of the stomach can remove accumulation of food and drink, promote the aging of food and cause the new disease. Typhoid fever, excessive heat in the abdomen, restlessness, diabetes, diuresis, and sore. Resolving hard masses, treating abdominal distention, pain, blood stasis, lower scrofula and diarrhea.

White petrolatum, HALLOYSITUM ALBUM, sweet, sour, flat, nontoxic. The main functions are as follows: it is an antidiarrheal with astringents. Nourishing lung qi, strengthening intestine, nourishing bone marrow, treating palpitation, vexation, relieving abdominal pain, running water, small intestine, loose stool, purulent blood, metrorrhagia, metrostaxis, deep-colored leucorrhea, and carbuncle, sore, hemorrhoid. Now commonly used externally as an absorbent.

Ricepaper pith, tetrapanax papyrifer (hook.) k.koch, aka koch, and the like; sweet in taste; light; slightly cold in nature; is nontoxic. The main indications of the lung entering, large intestine and small intestine three channels include: improving eyesight, reducing fever, promoting tissue regeneration, promoting lactation.

Radix Platycodi, platycodon grandiflorus (Jacq.) A.DC, sexual pungent and slightly warm, and crude platycodin has tranquilizing, analgesic and antipyretic effects. The main functions are as follows: disperse lung, relieve sore throat, eliminate phlegm and expel pus. Can be used for treating cough with excessive phlegm, chest distress, and suppuration.

Leonurus, leonurus species, has slightly bitter and cold taste, has the effects of activating blood circulation to dissipate blood stasis, diminishing inflammation, clearing heat and detoxicating, and is an active ingredient leonurine.

Gypsum Fibrosum, gypsum fibraosum, is sweet and pungent in flavor and is cold in nature. Enter lung and stomach meridians. The main functions are as follows: clearing heat and purging fire, relieving restlessness and quenching thirst; calcining: promoting wound healing, promoting tissue regeneration, eliminating dampness and stopping bleeding.

Rhizoma anemarrhenae, anemarrhena asphodeloides Bunge, bitter, cold, enter lung, stomach, kidney meridians, are mainly used for: polydipsia due to fever, dryness and cough due to lung heat, hectic fever due to bone steaming, internal heat and diabetes, constipation due to intestinal dryness.

Prepared rhubarb, rheum officinale Baill, mainly used for the function: purgation and accumulation, clearing heat and purging fire, cooling blood and detoxicating, removing blood stasis and dredging channels.

Sodium bicarbonate is odorless, alkali is easy to dissolve in water, and can make up for the shortage of lysine content in feed.

The feed additive containing the traditional Chinese medicine and probiotics for promoting lactation and the preparation method thereof are prepared by adopting a traditional Chinese medicine formula according to the common postpartum problem of sows, wherein the traditional Chinese medicine comprises the following components: the functions of the shizu milk and the medulla Tetrapanacis are lactation promotion, the functions of the niter, the motherwort, the platycodon grandiflorum and the white stone are anti-inflammation, antivirus, heat-clearing and detoxicating and promoting lochia discharge, the functions of the gypsum, the rhizoma anemarrhenae, the prepared rhubarb and the sodium bicarbonate are stomach fire removal, blood cooling and difficult defecation relief, and the liquorice is the cool property of the neutralized gypsum, the rhizoma anemarrhenae and the prepared rhubarb.

The efficacy of each strain in the probiotics is as follows: the lactobacillus can secrete vitamins, and has therapeutic effect on constipation; the yeast has digestion promoting effect; bacillus can colonize the intestinal tract, promote the balance of intestinal flora and assist in nutrition absorption; enterococcus faecalis can secrete natural antibiotics and probiotics, and can inhibit the growth of escherichia coli and salmonella in intestinal tracts; bacillus coagulans can be planted in the second half of intestinal tract, and simultaneously secrete amino acids, vitamins and various digestive enzymes, thereby being beneficial to the health of organisms.

The efficacy of the various enzymes in the enzyme preparation is: the cellulose can convert insoluble cellulose into glucose and destroy the cell walls of the traditional Chinese medicine plants, so that the release of the medicinal components in the cells of the traditional Chinese medicine plants is improved, the content and the absorption of the medicinal components of the traditional Chinese medicine are improved, and the medicinal effect of the traditional Chinese medicine is further improved; xylanase mainly degrades heterogeneous polysaccharide of the cell wall of the traditional Chinese medicine plant and degrades hemicellulose, so that the medicinal components in the cell wall of the traditional Chinese medicine plant are released; the glucose oxidase can regulate the glucose content in blood sugar, thereby improving the feed intake of animals and increasing the lactation energy source.

According to the invention, the konjak mannan is regulated by adopting sodium hydroxide, hydrogen peroxide is added for reaction, sodium bisulfate is adopted for terminating reaction, tapioca starch is doped, and modified powder is obtained after dry heat treatment, so that the modified powder has low solubility and swelling capacity, and is beneficial to loading active ingredients. And the doping of the tapioca starch obviously reduces the porosity and the swelling rate of the modified powder, delays the release of the medicine and has better slow release performance. Then the shrimp and crab shell is used for preparing the extract as a cross-linking agent of sodium alginate powder, so that the auxiliary agent is successfully prepared. The sodium alginate powder and the L-histidine have calcium ion crosslinking, polyelectrolyte interaction and hydrogel bond forming interaction with glycerin and the extract, and then are mixed with lactose and starch, so that the thermal stability of the outer coating film can be improved, the strength, mechanical property and thermal stability of the auxiliary agent coated drug carrier are improved, and the sustained release capability of the drug can be shown. In addition, the traditional Chinese medicine prepared by the spray process has good roundness, good fluidity and good stability, can slowly release medicines in sow intestinal juice, and improves the oral bioavailability of sows.

Compared with the prior art, the invention has the beneficial effects that:

1) The traditional Chinese medicine formula prepared by the invention adopts a plurality of traditional Chinese medicines to divide into two doses, respectively solves the problems of postpartum hypogalactia and subsequent difficult defecation of sows, and has the effects of resisting bacteria, diminishing inflammation, resisting viruses, improving the immunity of organisms, regulating intestinal flora and promoting intestinal digestion and absorption;

2) The strength, mechanical property and thermal stability of the coated drug carrier are improved by preparing the modified powder and the auxiliary agent, the traditional Chinese medicine prepared by the spray process has good roundness, good fluidity and good stability, can slowly release the drug in the intestinal juice of the sow, shows the capability of continuously releasing the drug, and improves the oral bioavailability of the sow.

Detailed Description

The main material sources are as follows:

saltpeter: xinjiang and the field of the producing area.

Medulla Tetrapanacis: elm in the producing area.

Radix Platycodi: the producing area is Hubei Yingshan.

Rhizoma anemarrhenae: the producing area is Gansu Minxian county.

Motherwort: ou city of Fujian province.

White stone grease: the east yang city of Zhejiang.

Gypsum: jujube village in the Shandong province. The Liaoning province wide-pattern full-family county.

Stone clock emulsion: the producing area is Sichuan river weir.

Preparing rheum officinale: gansu Long xi of producing places.

Licorice root: the producing area is the Shangxi Shuo state.

Shrimp and crab shells, lin_26384, county Hua feed Co., ltd., product number: XKF0261.

Konjak mannan: sian Siji Biotech Co., ltd., product number: SJMYPGLJT.

Lactobacillus plantarum: latin name: lactobacillus plantarum, strain number: CICC22209.

Bacillus subtilis: latin name Bacillus subtilis, strain number: CICC 25064.

Saccharomyces cerevisiae: latin name: saccharomyces cerevisiae strain number: CICC32883.

Enterococcus faecalis: latin name: enterococcus faecalis, strain number: CICC20396.

Bacillus coagulans, latin name: bacillus coagulans, strain number: ATCC7050 (american type culture collection).

Cellulase, jiangsu Yan Kogyo Co., ltd., product number: 1011, enzyme activity: 10 ten thousand;

xylanase, guangzhou Hua Xi biological Co., ltd., product number: 101, enzyme activity: 1 ten thousand;

glucose oxidase, novelin investment limited, model: gluzyme Mono10000B, enzyme Activity: 10000GODU/g;

example 1

A traditional Chinese medicine formula for treating postpartum hypogalactia of sows is prepared by the following steps:

t1, adding 2kg of active ingredients into 10kg of water, magnetically stirring for 30min at the stirring speed of 600rpm to obtain a liquid medicine, spraying the liquid medicine on the surface of 1kg of modified powder in a fluidized bed, and then drying at 60 ℃ for 15min to obtain a medicine carrier; the operation parameters of the fluidized bed are set to be sprayMist speed 4g/min, atomizing air pressure 2.0bar, material temperature 32 ℃, inlet temperature 50 ℃ and air spraying speed 19m ³ /h；

T2, adding 1.5kg of auxiliary agent into 10kg of water, adding 0.5kg of lactose and 1kg of tapioca starch, stirring for 30min at a stirring speed of 200rpm to prepare slow-release coating liquid, spraying the slow-release coating liquid on the surface of the medicine carrying body prepared in the step T1 through a fluidized bed, wherein the operation parameters of the fluidized bed are set to be that the spraying speed is 2g/min, the atomization air pressure is 1.44bar, the material temperature is 27.5 ℃, the inlet temperature is 35 ℃, and the spraying air velocity is 15.5m ³ And/h, drying at 40 ℃ for 24h to obtain the traditional Chinese medicine formula.

The preparation method of the active ingredient comprises the following steps:

step 1, weighing 2kg of stone bell milk, 2kg of saltpeter, 2kg of white stone, 5kg of medulla Tetrapanacis, 6kg of platycodon grandiflorum and 5kg of motherwort; grinding in a grinder for 2h to obtain a traditional Chinese medicine mixture A;

step 2, weighing 4kg of gypsum, 3kg of rhizoma anemarrhenae, 5kg of prepared rheum officinale, 3kg of sodium bicarbonate and 1.5kg of liquorice, and grinding in a grinder for 2 hours to obtain a traditional Chinese medicine mixture B;

and 3, mixing the traditional Chinese medicine mixture A prepared in the step 1 with the traditional Chinese medicine mixture B prepared in the step 2, grinding for 3 hours, sieving with a 80-mesh sieve to obtain traditional Chinese medicine mixed powder, adding 0.4kg of probiotic powder, adding 0.18kg of complex enzyme preparation powder, stirring for 30 minutes at a stirring speed of 100rpm, drying at 40 ℃, and controlling the water content to be 7wt%, thereby obtaining the active ingredient.

The probiotic powder is freeze-dried powder and consists of the following strains: lactobacillus plantarum 0.075kg, bacillus subtilis 0.1kg, saccharomyces cerevisiae 0.075kg, enterococcus faecalis 0.075kg, and Bacillus coagulans 0.075kg.

The composite enzyme preparation powder is dry powder and consists of the following enzyme preparations: cellulase 0.08kg, xylanase 0.05kg and glucose oxidase 0.05kg.

The preparation method of the auxiliary agent comprises the following steps:

s1, crushing 4kg of shrimp and crab shells into fine particles by using a superfine crusher, sieving by using a 200-mesh sieve, adding into 20kg of 5wt% sodium hydroxide aqueous solution, stirring for 5 hours at 65 ℃, adding 5kg of absolute ethyl alcohol at 200rpm, stirring for 6 hours at room temperature, stirring at 400rpm, filtering by using a 400-mesh sieve, collecting solid, washing with water until washing water is neutral, and drying for 12 hours at 60 ℃ in a vacuum oven to obtain a pretreated substance;

s2, adding 1kg of the pretreated matter prepared in the step S1 into 2.5kg of 33wt% sodium hydroxide aqueous solution, stirring for 3 hours at 100 ℃, cooling to room temperature at 200rpm, cooling by using an ice water bath, sieving with a 400-mesh sieve, collecting solids, and washing with water for 2 times to obtain an extract;

s3, adding 0.6kg of L-histidine into 3kg of water to obtain an L-histidine water solution; adding 0.6kg of sodium alginate powder into 5kg of water at 25 ℃, and stirring for 2 hours to obtain a homogeneous solution; adding 0.3kg of the extract prepared in the step S2 into the homogeneous solution, then adding 0.06kg of glycerol, stirring at 500rpm for 2 hours, adding the L-histidine aqueous solution, stirring for 20 minutes at a stirring speed of 200rpm, evaporating at 45 ℃ for 72 hours, then placing in an incubator at a temperature of 25 ℃ and a relative humidity of 50%, and preserving for 48 hours to obtain the auxiliary agent.

The preparation method of the modified powder comprises the following steps:

z1, adding 1.8kg of konjak mannan into 10kg of water, stirring at 45 ℃ for 30min at 500rpm, adjusting the pH to 8.0 by using 1wt% sodium hydroxide aqueous solution, adding 0.4kg of hydrogen peroxide every 10min, adding 3 times, adding 1mol/L sodium bisulfate aqueous solution after 4h to adjust the pH to 6.5, precipitating by using absolute ethyl alcohol, filtering by a vacuum filter, washing 3 times by using 70wt% ethanol aqueous solution, drying at 50 ℃ in vacuum for 2h, and grinding by a 100-mesh sieve to obtain pretreated powder;

z2, adding 1kg of the pretreated powder prepared in the step Z1 into 4kg of water, slowly adding 0.2kg of tapioca starch, stirring at room temperature for 1h at a stirring speed of 200rpm, drying at 45 ℃ until the water content is 10wt%, further grinding, sieving through a 100-mesh sieve, and heating at 130 ℃ for 4h to obtain the modified powder.

Example 2

The preparation method of the traditional Chinese medicine formula for treating postpartum hypogalactia of sows is basically the same as that of the embodiment 1, and the only difference is that: the preparation method of the traditional Chinese medicine formula is inconsistent.

A preparation method of a traditional Chinese medicine formula for treating postpartum hypogalactia of sows comprises the following steps: adding 1.5kg of auxiliary agent into 10kg of water, adding 0.5kg of lactose and 1kg of tapioca starch, stirring for 30min at a stirring speed of 200rpm to prepare slow-release coating liquid, spraying the slow-release coating liquid on 2kg of active ingredient surface by a fluidized bed, wherein the operation parameters of the fluidized bed are set to be spraying speed of 2g/min, atomizing air pressure of 1.44bar, material temperature of 27.5 ℃, inlet temperature of 35 ℃ and spraying air speed of 15.5m ³ And/h, drying at 40 ℃ for 24h to obtain the traditional Chinese medicine formula.

The preparation method of the active ingredient is the same as in example 1.

The preparation method of the auxiliary agent is the same as that of the example 1.

Example 3

A preparation method of a traditional Chinese medicine formula for treating postpartum hypogalactia of sows comprises the following steps:

t1, adding 2kg of active ingredients into 10kg of water, magnetically stirring for 30min at the stirring speed of 600rpm to obtain a liquid medicine, spraying the liquid medicine on the surface of 1kg of modified powder in a fluidized bed, and then drying at 60 ℃ for 15min to obtain a medicine carrier; the operation parameters of the fluidized bed are set to be the spraying speed of 4g/min, the atomization air pressure of 2.0bar, the material temperature of 32 ℃, the inlet temperature of 50 ℃ and the air spraying speed of 19m ³ /h；

T2, adding 0.5kg lactose and 1kg tapioca starch into 10kg water, stirring for 30min at 200rpm to obtain slow-release coating liquid, spraying the slow-release coating liquid on the surface of the medicine carrier prepared in the step T1 by a fluidized bed, wherein the operation parameters of the fluidized bed are set to be spraying speed of 2g/min, atomization air pressure of 1.44bar, material temperature of 27.5 ℃, inlet temperature of 35 ℃ and spraying air speed of 15.5m ³ And/h, drying at 40 ℃ for 24h to obtain the traditional Chinese medicine formula.

The preparation method of the active ingredient is the same as in example 1.

The preparation method of the modified powder is the same as in example 1.

Example 4

T2, adding 1.5kg of auxiliary agent into 10kg of water, stirring for 30min at a stirring speed of 200rpm to prepare slow-release coating liquid, spraying the slow-release coating liquid on the surface of the medicine carrier prepared in the step T1 by a fluidized bed, wherein the operation parameters of the fluidized bed are set to be spraying speed of 2g/min, atomizing air pressure of 1.44bar, material temperature of 27.5 ℃, inlet temperature of 35 ℃ and spraying air velocity of 15.5m ³ And/h, drying at 40 ℃ for 24h to obtain the traditional Chinese medicine formula.

The preparation method of the active ingredient is the same as in example 1.

The preparation method of the modified powder is the same as in example 1.

Comparative example 1

The preparation method of the traditional Chinese medicine formula for treating postpartum hypogalactia of sows is basically the same as that of the embodiment 1, and the only difference is that: the traditional Chinese medicine formula is replaced by an active ingredient.

The preparation method of the active ingredient is the same as in example 1.

Test example 1

Milk ingredient test

Test site: the pig farm of Jiangxi Jinggang mountain Hua Fu is a binary sow, and the sows are pregnant for the second time, and the clinical effects of the traditional Chinese medicine formula for treating postpartum hypogalactia of the sows are tested by arranging blank control, milk-poor sows and milk-poor sows in a medicine group, wherein the medicine group of the milk-poor sows is 5 groups, and the traditional Chinese medicine formula prepared by adopting the embodiment and the comparative example is fed in an amount of 1% of the mass of feed per day, and 20 heads of the feed are fed in each group. Colostrum components, normal milk components, lactation period and lactation amount were tested in g/100g, and the average value was taken, and the test statistical results are shown in tables 1, 2 and 3.

TABLE 1 colostrum composition table test results

TABLE 2 results of the normal milk ingredient test

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TABLE 3 lactation cycle and lactation test results

Test example 2

Sow defecation difficulty rate determination

By counting the defecation difficulty rate of the sow in the lactation period in the test example 1, the number of constipation sows was recorded, the number of the sow with the defecation difficulty was recorded, and the test results are shown in table 4.

Table 4: sow defecation difficulty rate test result

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From tables 1 to 4, it can be seen that the effect of example 1 on treating postpartum hypogalactia of sow is best, and the effect of improving constipation is good, and the main reasons are probably that the stone-like holly and medulla tetrapanacis have lactation promoting function, the niter, the motherwort, the platycodon grandiflorum and the white stone have the functions of diminishing inflammation, resisting virus, clearing heat and detoxicating and promoting lochia discharge, the gypsum, the rhizoma anemarrhenae, the prepared rheum officinale and the sodium bicarbonate have the functions of dispelling stomach fire, cooling blood, relieving difficult defecation and the liquorice has the cooling property of neutralizing the gypsum, the rhizoma anemarrhenae and the prepared rheum officinale. The lactobacillus in the probiotics can secrete vitamins, and has therapeutic effect on constipation; saccharomyces cerevisiae has digestion promoting effect; bacillus can colonize the intestinal tract, promote the balance of intestinal flora and assist in nutrition absorption; enterococcus faecalis can secrete natural antibiotics and probiotics, and can inhibit the growth of escherichia coli and salmonella in intestinal tracts; bacillus coagulans can be planted in the second half of intestinal tract, and simultaneously secrete amino acids, vitamins and various digestive enzymes, thereby being beneficial to the health of organisms. In addition, the cellulose can convert insoluble cellulose into glucose and destroy the cell walls of the traditional Chinese medicine plants, so that the release of the medicinal components in the cells of the traditional Chinese medicine plants is improved, the content and the absorption of the medicinal components of the traditional Chinese medicine are improved, and the medicinal effect of the traditional Chinese medicine is further improved; xylanase mainly degrades heterogeneous polysaccharide of the cell wall of the traditional Chinese medicine plant and degrades hemicellulose, so that the medicinal components in the cell wall of the traditional Chinese medicine plant are released; the glucose oxidase can regulate the glucose content in blood sugar, thereby improving the feed intake of animals and increasing the lactation energy source.

The konjak mannan is regulated by adopting sodium hydroxide, hydrogen peroxide is added for reaction, sodium bisulfate is adopted for terminating reaction, tapioca starch is doped, modified powder is obtained after dry heat treatment, and the modified powder has low solubility and swelling capacity, so that the modified konjak mannan is favorable for loading active ingredients. And the doping of the tapioca starch obviously reduces the porosity and the swelling rate of the modified powder, delays the release of the medicine and has better slow release performance. Then the shrimp and crab shell is used for preparing the extract as a cross-linking agent of sodium alginate powder, so that the auxiliary agent is successfully prepared. The sodium alginate powder and the L-histidine have calcium ion crosslinking, polyelectrolyte interaction and hydrogel bond forming interaction with glycerin and the extract, and then are mixed with lactose and starch, so that the thermal stability of the outer coating film can be improved, the strength, mechanical property and thermal stability of the auxiliary agent coated drug carrier are improved, and the sustained release capability of the drug can be shown. In addition, the traditional Chinese medicine prepared by the spray process has good roundness, good fluidity and good stability, can slowly release medicines in sow intestinal juice, and improves the oral bioavailability of sows.

Claims

1. An active ingredient is characterized by comprising the following raw materials: stone bell milk, saltpeter, white stone, medulla Tetrapanacis, platycodon grandiflorum, motherwort, gypsum, rhizoma anemarrhenae, prepared rheum officinale, sodium bicarbonate, liquorice, composite probiotics and a composite enzyme preparation;

the preparation method of the active ingredient comprises the following steps:

2. The active ingredient according to claim 1, comprising the following raw materials in parts by weight: 10-30 parts of stone clock emulsion, 10-30 parts of saltpeter, 10-30 parts of white stone, 20-90 parts of medulla Tetrapanacis, 40-80 parts of platycodon grandiflorum, 30-80 parts of motherwort, 30-60 parts of gypsum, 30-60 parts of rhizoma anemarrhenae, 30-80 parts of prepared rheum officinale, 10-50 parts of sodium bicarbonate, 10-20 parts of liquorice, 3-5 parts of compound probiotics and 1-2 parts of compound enzyme preparation;

the preparation method of the active ingredient comprises the following steps:

3. The active ingredient according to claim 1 or 2, wherein the probiotic powder is a lyophilized powder, consisting of the following species in parts by weight: 1 to 2 parts of lactobacillus plantarum, 2 to 3 parts of bacillus subtilis, 1 to 2 parts of saccharomyces cerevisiae, 1 to 2 parts of enterococcus faecalis and 1 to 2 parts of bacillus coagulans.

4. The active ingredient according to claim 1 or 2, wherein the complex enzyme preparation powder is a dry powder, and consists of the following enzyme preparations in parts by weight: 3-5 parts of cellulase, 2-3 parts of xylanase and 2-3 parts of glucose oxidase.

5. The preparation method of the traditional Chinese medicine formula for treating postpartum hypogalactia of sows is characterized by comprising the following steps of:

t1, adding 1-3 parts of the active ingredient in any one of claims 1-4 into 8-12 parts of water, magnetically stirring for 20-50 min at a stirring speed of 400-800 rpm to obtain a liquid medicine, spraying the liquid medicine on the surface of 0.5-2 parts of modified powder in a fluidized bed, and then drying at 40-70 ℃ for 10-20 min to obtain a medicine carrying body; the operation parameters of the fluidized bed are set to be the spraying speed of 2-5 g/min, the atomization air pressure of 1.0-3.0 bar, the material temperature of 30-40 ℃, the inlet temperature of 40-70 ℃ and the spraying air velocity of 10-30 m ³ /h；

6. The method for preparing the traditional Chinese medicine formula for treating postpartum hypogalactia of sows as claimed in claim 5, wherein the preparation method of the auxiliary agent is as follows:

s3, adding L-histidine into water to obtain an L-histidine water solution, adding sodium alginate powder into water, and stirring to obtain a homogeneous solution; and (3) adding the extract prepared in the step (S2) into a homogeneous solution, then adding glycerol, stirring, adding an L-histidine aqueous solution, stirring again, evaporating, and then placing in an incubator for storage to obtain the auxiliary agent.

7. The method for preparing the traditional Chinese medicine formula for treating postpartum hypogalactia of sows as claimed in claim 5, wherein the preparation method of the modified powder is as follows:

z1, adding konjak mannan into water, stirring, adjusting the pH with a sodium hydroxide aqueous solution, adding hydrogen peroxide, adding a sodium bisulfate aqueous solution to adjust the pH, precipitating with absolute ethyl alcohol, filtering, cleaning, drying, grinding and sieving to obtain pretreated powder;

8. A traditional Chinese medicine formula for treating postpartum hypogalactia of sows, which is characterized by being prepared by adopting the method as claimed in any one of claims 5-7.

9. The preparation method of the auxiliary agent is characterized by comprising the following steps of:

10. The preparation method of the modified powder is characterized by comprising the following steps of: