CN103865854B - A kind of compound micro-ecological preparation and preparation method thereof - Google Patents
A kind of compound micro-ecological preparation and preparation method thereof Download PDFInfo
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- CN103865854B CN103865854B CN201410103781.7A CN201410103781A CN103865854B CN 103865854 B CN103865854 B CN 103865854B CN 201410103781 A CN201410103781 A CN 201410103781A CN 103865854 B CN103865854 B CN 103865854B
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Abstract
The invention discloses a kind of compound micro-ecological preparation and preparation method thereof, this compound micro-ecological preparation includes bacillus cereus, bacillus subtilis and lactobacillus casei, and the bacterial concentration ratio of described bacillus cereus, bacillus subtilis and lactobacillus casei is 1:1～3:1～2.5.Present invention advantage compared to existing technology is: this compound micro-ecological preparation is to be mixed with bacillus cereus by lactobacillus casei, has given full play to the synergism between bacterial strain, the aerobic metabolism of lactic acid bacteria is provided protection, improve the utilization rate of equipment.
Description
Technical field
The present invention relates to a kind of microbial ecological agent, in particular a kind of compound micro-ecological preparation and preparation method thereof.
Background technology
In recent years, along with the additive such as antibiotic and chemicals being widely used in livestock and poultry breeding industry, people are own through gradually anticipating
Knowing all negative effects brought to antibiotic and chemicals, such as antibiotic causes animal normal flora to be lacked of proper care, great Liang Nai
Medicated strain produces；The toxic and side effects such as drug residue, brings serious harm to animal, the mankind and environment, and drops simultaneously
Low animal products competitiveness commercially, therefore, many countries are prevented or restricted from the application in feedstuff such as antibiotic in succession.
In view of all adverse effects of antibiotic, research worker both domestic and external is devoted to develop some always and puts down by adjusting animal gastrointestinal tract
Weighing apparatus and function, to reach promote growth and maintain the new additive agent of the purposes such as animal health level, animal microecological formulation is because of it
Have growth promotion, nontoxic, have no side effect, the feature such as noresidue and cause extensive concern and the attention of various countries, the kind of use
More and more, range of application is the most quite varied, and its good result being applied to animal productiong is own by lot of experiments and production practices institute
Confirm.
Microbial ecological agent is under the guidance of microecology theory, adjusts microbial state treatment, keeps microecological balance to improve host and is good for
The class preparation that Kang Shuiping or promotion probiotics growth and metabolite thereof produce.According to definition, microbial ecological agent includes prebiotic
Bacterium, three concepts of prebiotics and symphysis unit.In recent years, developing multiple probiotics preparation both at home and abroad, its guiding theory is employment
Or the member of animal normal physiological flora, through seed selection and artificial propagation, make active bacteria formulation by all means, the most again with
Investing method is returned to former environment, plays natural physiological action.Probiotic bacteria in use has promotion probiotics propagation, presses down
The multiple effects such as pathogen processed, enhancing human body immunity power, are widely used the most at short notice.
Bacillus cereus be a kind of aerobic, harmless, can produce spore, acid and alkali-resistance, high temperature resistant and extruding antibacterial, at intestinal acidity
Environment has the stability of height；It can promote the growth of probiotics, the harmful bacteria in antagonism intestinal, enhancing human body immunity
Power, improves resistance against diseases；It addition, it can also secrete more strongly active protease and amylase, growth of animal speed can be significantly improved
Degree, promotes the digestion of feed nutrition material, but it can not definite value in intestinal, belong to intestinal and pass by one's way bacterium.Report is more at present
Bacillus has the probiotics kinds such as bacillus subtilis, Bacillus licheniformis, bacillus cereus and Japan's bacillus cereus
Lactic acid bacteria is that a kind of saccharide that can decompose produces the gram positive bacteria of lactic acid, anaerobism or amphimicrobian growth.Animal
Internal by Ant agonism reduction pH value, stop and suppress intrusion and the definite value of pathogenic bacterium；Degraded ammonia, indole, scatol
Deng harmful substance, maintain normal ecological balance in intestinal；Strengthen humoral immunization and cellular immunization；Can be used for suckling and weaning stage
In the feedstuff of animal.The lactic acid bacteria of application arises primarily at Lactobacillus, nisin Pseudomonas and Bifidobacterium at present
Nearly 30 kinds of microorganisms, the Ministry of Agriculture of China allows the lactic acid bacteria being used as feed additive to have: lactobacillus casei, streptococcus faecalis, dung chain
Coccus, pediococcus acidilactici, bacillus acidophilus and Streptococcus lactis (Lister) Lohnis 1909.554..
Lactic acid bacteria is the antibacterial that a big class can produce acid from fermentable carbohydrates, for the microflora in intestinal, for anaerobe or
Facultative anaerobe.In growth course, lactic acid bacteria is formed without spore, and resistance is poor, thus stores at feed granulating and preparation
Journey easily inactivates.For improving resistance, preserve the long period, by its micro encapsulation, but life can be improve in process of production
Producing cost, therefore in feed industry, application receives some restrictions.
Lactobacillus casei and the mixed culture of bacillus cereus in prior art, owing to lactobacillus casei is facultative anaerobe, and it is raw
Nutrient substance needed for length is more complicated than bacillus cereus, if be directly mixed, each bacterial strain fails to absorb preferable nutrition, bacterium
Strain activity is not fine, and then the effect of the compound micro-ecological preparation obtained is the most notable.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of compound micro-ecological preparation and preparation method thereof, with
A kind of lactobacillus casei and the mixed culturing method of bacillus cereus and a kind of new high activity microbial ecological agent are provided.
The present invention is achieved by the following technical solutions:
A kind of compound micro-ecological preparation, described compound micro-ecological preparation includes bacillus cereus, bacillus subtilis and cheese milk
Bacillus, the bacterial concentration ratio of described bacillus cereus, bacillus subtilis and lactobacillus casei is 1:1～3:1～2.5.
Preferably, the bacterial concentration ratio of described bacillus cereus, bacillus subtilis and lactobacillus casei is 1:3:2.
Preferably, described bacillus cereus and bacillus subtilis are screened from animal alimentary canal cecal content and obtain,
Especially screening obtains from the night blindness intestinal contents, it is also possible to common bacillus cereus and bacillus subtilis substitute.
A kind of method preparing above-mentioned compound micro-ecological preparation, comprises the following steps:
(1) fetching number Later growth is to the lactobacillus casei in stable phase later stage, is inoculated in MRS culture medium, anaerobism quiescent culture
24 ± 2 hours, it is thus achieved that lactobacillus casei culture fluid；
(2) fetching number Later growth is to the bacillus subtilis in stable phase later stage and bacillus cereus, is inoculated into LB training respectively
Supporting in base, concussion cultivation 12 ± 2 hours, described bacillus subtilis and bacillus cereus are preferably in animal alimentary canal caecum
Tolerant middle screening obtains, because the bacillus subtilis thalline of screening can be sprouted in being formed in growth course from digestive tract caecum
Born of the same parents, it is after the field planting of intestinal short time, can consume substantial amounts of oxygen, maintains intestinal anaerobic environment, strengthens intestinal to anaerobe
Field planting, suppression pathogenic microorganism simultaneously facilitates the growth of the beneficial microorganism such as lactic acid bacteria, and produces the organic acids such as lactic acid, fall
Low gut pH, suppresses the growth of other pathogenic bacterium indirectly, and meanwhile, the bacillus subtilis thalline in digestive tract can self synthesis
Digestibility enzyme, such as protease, amylase, lipase etc., plays a role with endogenous enzymes in digestive tract jointly, improves feedstuff
Digestibility；It addition, the bacillus cereus in digestive tract caecum can not only produce endospore, and spore can be high temperature resistant, energy
Enough tolerate gastric juice, the digestion of intestinal juice and the impact of bile, there is stronger intestinal colonisation power, it is possible in intestinal, maintain a fixed number
The viable count of magnitude；In the present invention, bacillus subtilis and bacillus cereus also can replace with any commercially available strain；
(3) first by step (2) concussion cultivate after bacillus cereus and bacillus subtilis in bacterial concentration ratio 1～4:
1～the 4 compound proportionings carrying out different proportion, measure bacillus cereus mixed liquor under different ratio and produce amylase and the content of protease,
Amylase and the high best complex ratio of protease content are produced in screening, and the best complex ratio of screening is 1:1～3, will concussion
After bacillus cereus and bacillus subtilis in bacterial concentration ratio 1:1～3 mixing, it is thus achieved that mixed liquor, mixed liquor is connect
Planting in LB fluid medium, at 37 ± 1 DEG C, 220rpm continues concussion cultivation 14 ± 2h, it is thus achieved that bacillus cereus culture fluid；
(4) first the bacillus cereus culture fluid of the lactobacillus casei culture fluid of step (1) Yu step (3) is carried out different proportion
Compound proportioning, the proportion of described compound proportioning is: the antibacterial of bacillus cereus, bacillus subtilis and lactobacillus casei
Concentration ratio 1:1～3:1～4, measure mixed bacteria liquid under different composite proportioning and produce amylase and the content of protease, filter out
Producing amylase and the high best complex ratio of protease content, described best complex ratio is: bacillus cereus, hay spore
Bacterial concentration ratio 1:1 of bacillus and lactobacillus casei～3:1～2.5；Lactobacillus casei culture fluid is cultivated with bacillus cereus
Liquid is mixed in best complex ratio, bacillus cereus, bacillus subtilis and lactobacillus casei in mixed culture fluid
Bacterial concentration ratio be 1:1～3:1～2.5, be described compound micro-ecological preparation.
Preferably, in described step (3), bacillus cereus and bacillus subtilis are mixed in bacterial concentration ratio 1:3,
In described step (4), the bacterial concentration ratio of bacillus cereus, bacillus subtilis and lactobacillus casei is 1:3:2.
Preferably, in described step (3), the inoculum concentration of mixed liquor is the LB fluid medium inoculation 1mL mixing of every 100mL
Liquid.
The principle of the present invention is: the present invention first determine the bacteriocinogeny of lactobacillus casei higher time growth time be 24～
48h, the growth time that mixing bacillus cereus yield of enzyme is higher is 14～16h, is then under anaerobic cultivated by lactobacillus casei
After 24h, then by cultivate under aerobic condition the bacillus cereus of 14h and bacillus subtilis mixed liquor and lactobacillus casei by than
Example mixes, and continues cultivation 10 ± 2h, obtains the preferable microbial ecological agent of composite effect, then by the compound proportioning of different proportion,
Filter out the best complex ratio of three kinds of antibacterials, obtain producing amylase and producing the compound strain that protease content is high.
The present invention has the advantage that compared to existing technology and the invention provides a kind of compound micro-ecological preparation and preparation method thereof,
The preparation method of described compound micro-ecological preparation is liquid mixing fermentation culture, compensate for the defect of solid state fermentation culture, it is achieved that
Many bacterial strains are total to fermentation culture simultaneously, and preparation is simple, low cost；The bacterium solution of fermentation is directly used in animal feed, reaches probiotics
Efficient combination, makes the premium properties of various probiotics be not fully exerted；Described microbial ecological agent can be used directly to feed chickling,
The aspects such as the production performance of chicken, immune performance, intestinal pH, enteric microorganism there are is improvement, may advantageously facilitate the growth of chicken,
Improve immunity, increase the digestibility of feedstuff；It addition, in described compound micro-ecological preparation bacteriostatic test in vitro, intestinal is caused
Pathogenic bacteria has preferable bacteriostatic activity, in animal feed experiment in vivo, in the case of without any antibiotic medicine,
Significantly improve average daily gain and the immune performance of white meat-type chickens, improve the flora environment of white meat-type chickens intestinal, promote white
The growth of plumage broiler.
Accompanying drawing explanation
Fig. 1 is lactobacillus casei growth curve chart；
Fig. 2 is bacillus subtilis growth curve chart；
Fig. 3 is Bacillus cereus growth curve chart；
Fig. 4 is bacillus cereus and protease production under bacillus subtilis different composite proportioning；
Fig. 5 is that bacillus cereus produces amylase activity under bacillus subtilis different composite proportioning；
Fig. 6 is protease production under bacillus cereus, bacillus subtilis and lactobacillus casei different composite proportioning；
Fig. 7 is product amylase activity under bacillus cereus, bacillus subtilis and lactobacillus casei different composite proportioning；
Detailed description of the invention
1～7 pair of embodiments of the invention elaborates below in conjunction with the accompanying drawings, and the present embodiment is premised on technical solution of the present invention
Under implement, give detailed embodiment and concrete operating process, but protection scope of the present invention be not limited to following
Embodiment.
Embodiment 1
1, culture medium preparation
YPD culture medium: include the yeast powder of 10g/L, the D of peptone B and 20g/L of 20g/L～anhydrous glucose, 115
Sterilizing 15 minutes at DEG C；
MRS culture medium: include the peptone of 10.0g/L, the beef powder of 8.0g/L, the yeast powder of 4.0g/L, 20.0g/L's
Glucose, the dipotassium hydrogen phosphate of 2.0g/L, the citric acid hydrogen diamine of 2.0g/L, the sodium acetate of 5.0g/L, the sulphuric acid of 0.2g/L
Magnesium, the manganese sulfate of 0.04g/L and the tween of 1.0g/L～80, at 25 DEG C, regulation pH value is to 5.7 ± 0.2, sterilizing at 118 DEG C
15 minutes；
LB culture medium: include the tryptone of 10.0g/L, the yeast leaching powder of 5.0g/L and 10.0g/L sodium chloride, 25 DEG C of downwards
Joint pH value to 7.0 ± 0.1, sterilizing 15 minutes at 121 DEG C.
Above culture medium adds the agar of 2g/L, is configured to solid medium.
2, preparation method
(1) cultivation of lactobacillus casei
Under aseptic condition, take lactobacillus casei freeze-drying lactobacillus, MRS solid medium is coated with, at 37 DEG C, stand Anaerobic culturel
20h, picking list bacterium colony, purification of ruling further on MRS solid medium, after 37 DEG C are cultivated 20h, picking list bacterium colony, connect
Kind in the MRS fluid medium of 100mL, anaerobism quiescent culture at 37 DEG C, respectively at 26h, 0h, 2h, 4h, 6h, 8h,
10h、12h、14h、16h、18h、20h、22h、24h、26h、28h、30h、32h、34h、36h、38h、40h,
Under absorbance 600nm measure OD value, obtain lactobacillus casei growth curve, described growth curve as it is shown in figure 1, from
It can be seen that lactobacillus casei is in the exponential growth later stage to the stable phase later stage when 24～48h, after taking exponential growth in Fig. 1
The lactobacillus casei bacterium solution in phase to stable phase later stage, is inoculated in MRS culture medium, 37 DEG C of anaerobism quiescent culture 24h, it is thus achieved that dry
Lactobacillus paracasei culture fluid；
(2) screening of bacillus cereus
Under aseptic condition, take 0.5g night blindness intestinal contents, add the physiological saline solution of 4.5mL, after fully concussion shakes up, put
80 DEG C of water-bath 10min, take 100 μ L bacterium solution, add in nutrient broth fluid medium, carry out again after 37 DEG C of shaken cultivation 24h
Than dilution, the dilution factor of doubling dilution is 10～4、10～5With 10～6, take the bacterium solution after 0.1mL doubling dilution respectively, coat
On YPD flat board, observe colonial morphology, then picking list bacterium colony, be inoculated in biochemical fermentation pipe cultivation 18～24h and observe, root
According to the reaction in biochemical identification pipe and colonial morphology, with reference to the 8th edition primary Jie Shi Bacteria Identification handbook, filter out bacillus subtilis
And bacillus cereus；
The bacillus subtilis filtered out and bacillus cereus are inoculated in the LB fluid medium of 50mL respectively, 37 ± 1
At DEG C, 220rpm shakes cultivation, respectively at 0h, 2h, 4h, 6h, 8h, 10h, 12h, 14h, 16h, 18h, 20h, 22h,
24h, 26h, 28h, 30h, 32h, 34h, 36h, 38h, 40h, measure OD value under absorbance 600nm, obtain two strains
Bacillus cereus growth curve, result as depicted in figs. 1 and 2, it can be seen that the index of bacillus subtilis is raw
The incubation time in long later stage to stable phase later stage is 12～20h, and the exponential growth later stage of bacillus cereus is to the stable phase later stage
Incubation time is 12～24h；
Fetching number Later growth, to the bacillus subtilis in stable phase later stage and bacillus cereus, is inoculated into LB culture medium respectively
In, at 37 ± 1 DEG C, 220rpm shakes cultivation 12 ± 2h；
(3) by step (2) concussion cultivate after bacillus cereus and bacillus subtilis in bacterial concentration ratio 1～4:
1～the 4 compound proportionings carrying out different proportion, it is thus achieved that the mixed liquor of different ratio, the mixed liquor taking different ratio respectively is inoculated into LB
Fluid medium is cultivated, the LB fluid medium inoculation 1mL bacterium solution of every 100mL, measure two kinds of bacillus cereus different proportions and mix
Producing amylase, protease content under conjunction, wherein the mensuration of protease uses Coomassie Brilliant Blue, and diastatic mensuration uses iodo-
Starch colorimetry, result as shown in Figure 4 and Figure 5, it can be seen that produce more optimal of amylase, protease content
Compositely proportional is 1:1～3；
Best complex ratio according to above-mentioned acquisition, presses the bacillus cereus after the concussion of step (2) and bacillus subtilis
Bacterial concentration ratio 1:1 mixes, it is thus achieved that mixed liquor, and the mixed liquor taking 1% volume ratio is inoculated in LB fluid medium, 37 ±
At 1 DEG C, 220rpm continues concussion cultivation 14 ± 2h, it is thus achieved that bacillus cereus culture fluid；
(4) the bacillus cereus culture fluid of the lactobacillus casei culture fluid of step (1) with step (3) is carried out answering of different proportion
Closing proportioning, the proportion of described compound proportioning is: the antibacterial of bacillus cereus, bacillus subtilis and lactobacillus casei is dense
Degree ratio 1:1～3:1～4, measure mixed bacteria liquid under different composite proportioning and produce amylase and the content of protease, and screening is produced
Amylase and the high best complex ratio of protease content, result as shown in Figure 6 and Figure 7, it can be seen that described
Good compositely proportional is: bacterial concentration ratio 1:1 of bacillus cereus, bacillus subtilis and lactobacillus casei～3:1～2.5；
According to above-mentioned best complex ratio, by the bacillus cereus culture fluid of step (1) lactobacillus casei culture fluid Yu step (3)
Mix in best complex ratio, bacillus cereus, bacillus subtilis and lactobacillus casei in mixed culture fluid
Bacterial concentration ratio is 1:1:1, is described compound micro-ecological preparation.
Embodiment 2
In the present embodiment, culture medium preparation is same as in Example 1, and preparation method comprises the following steps:
(1) cultivation of lactobacillus casei
Under aseptic condition, take lactobacillus casei freeze-drying lactobacillus, MRS solid medium is coated with, at 37 DEG C, stand Anaerobic culturel
20h, picking list bacterium colony, purification of ruling further on MRS solid medium, after 37 DEG C are cultivated 20h, picking list bacterium colony, connect
Planting in the MRS fluid medium of 100mL, quiescent culture 24h under anaerobic condition, now lactobacillus casei enters exponential growth
Later stage or stable phase later stage, take the bacterium solution after cultivation, be inoculated in MRS culture medium, 37 DEG C of anaerobism quiescent culture 24h, it is thus achieved that
Lactobacillus casei culture fluid；
(2) cultivation of bacillus cereus
Under aseptic condition, take 0.5g night blindness intestinal contents, add the physiological saline solution of 4.5mL, after fully concussion shakes up, put
80 DEG C of water-bath 10min, take 100 μ L bacterium solution, add in nutrient broth fluid medium, carry out again after 37 DEG C of shaken cultivation 24h
Than dilution, the dilution factor of doubling dilution is 10～4、10～5With 10～6, take the bacterium solution after 0.1mL doubling dilution respectively, coat
On YPD flat board, observe colonial morphology, then picking list bacterium colony, be inoculated in biochemical fermentation pipe cultivation 18～24h and observe, root
According to the reaction in biochemical identification pipe and colonial morphology, with reference to the 8th edition primary Jie Shi Bacteria Identification handbook, filter out bacillus subtilis
And bacillus cereus；
The bacillus subtilis filtered out and bacillus cereus are inoculated in the LB fluid medium of 50mL respectively, 37 ± 1
At DEG C, 220rpm concussion is cultivated, the bacillus subtilis of fetching number Later growth to stable phase later stage and bacillus cereus, then divides
Not being inoculated in LB fluid medium, at 37 ± 1 DEG C, 12h is cultivated in 220rpm concussion；
(3) bacillus cereus and bacillus subtilis after the concussion of step (2) being cultivated are mixed in bacterial concentration ratio 1:3
Closing, it is thus achieved that mixed liquor, the mixed liquor taking 1% volume ratio is inoculated in LB fluid medium continuation cultivation, it is thus achieved that bacillus cereus is trained
Nutrient solution；
(4) the bacillus cereus culture fluid of step (1) lactobacillus casei culture fluid with step (3) is mixed, mixed cultivation
In liquid, the bacterial concentration ratio of bacillus cereus, bacillus subtilis and lactobacillus casei is 1:3:2, is described compound
Microbial ecological agent.
Embodiment 3
In the present embodiment, the bacillus cereus of step (3) and the bacterial concentration ratio of bacillus subtilis are 1:3；Step
(4), in mixed culture fluid, the bacterial concentration ratio of bacillus cereus, bacillus subtilis and lactobacillus casei is 1:
3:2.5, remaining step and culture medium are prepared with embodiment 2.
Compound micro-ecological preparation embodiment 1～3 prepared feed 1 age in days white meat-type chickens chickling respectively, is divided into four by chickling
Group, often group 15, measured average daily gain in 1～42 day, and measure the white meat-type chickens Immune Organs Index when 42d,
In blank group, being replaced by microbial ecological agent use water, positive control is lactic acid bacteria, bacillus cereus, and yeast zinc, yeast selenium is multiple
Closing the compound micro-ecological preparation of preparation, measurement result is as shown in table 1 below:
Table 1: compound micro-ecological preparation is to white meat-type chickens average daily gain and the impact analysis of Immune Organs Index
From table 1 it follows that after the microbial ecological agent feed white meat-type chickens of embodiment 2, the average daily gain ratio of white meat-type chickens
Blank group improves 23.5%, the average daily gain to white meat-type chickens chick of the microbial ecological agent of embodiment 1 and embodiment 3
6.3% and 9.1% have been respectively increased it than blank group；Compared with positive controls, the average daily gain of positive controls improves
11.7%, and embodiment 2 improves 11.8% than the average daily gain of positive controls.
The data of the white meat-type chickens Immune Organs Index when 42d show, after the microbial ecological agent feed white meat-type chickens of embodiment 2,
The thymus index of white meat-type chickens, index and spleen index and bursal index have been respectively increased 42.8%, 35.5% and compared to blank
53.5%, and the thymus index of the white meat-type chickens under the microbial ecological agent feed of embodiment 1 and embodiment 3 has been respectively increased 12.6%
With 29.1%, index and spleen index has been respectively increased 5.5% and 22.7%, and bursal index has been respectively increased 9.1% and 14.1%；With sun
Property matched group is compared, and the thymus index of embodiment 2, index and spleen index and bursal index improve than positive controls respectively
21.3%, 36.4% and 15.1%.
Result above is it can be seen that the compound micro-ecological preparation of the present invention, the especially microbial ecological agent described in embodiment 2, it is possible to
It is effectively improved the growth rate of animal, promotes the growth of animal body, make the utilization rate of feedstuff be greatly improved；Meanwhile, originally
The compound micro-ecological preparation of invention, the especially microbial ecological agent described in embodiment 2, the immunity improving animal is also had significantly
Effect.
Embodiment 4
In the present embodiment, bacillus subtilis and the bacillus cereus of step (2) substitute with common commercially available bacterial strain, by ordinary city
After selling bacterial strain activation, being inoculated in LB fluid medium, fetching number Later growth, to the bacterium solution in stable phase later stage, is inoculated into LB
In fluid medium, at 37 ± 1 DEG C, 10h is cultivated in 220rpm concussion；The bacillus cereus of step (3) and bacillus subtilis
Bacterial concentration ratio is 1:2；In the mixed culture fluid of step (4), bacillus cereus, bacillus subtilis and dry
The bacterial concentration ratio of Lactobacillus paracasei is 1:2:2.5, and remaining step and culture medium are prepared with embodiment 2.
Claims (4)
1. a compound micro-ecological preparation, it is characterised in that described compound micro-ecological preparation is by bacillus cereus, hay spore
Bacillus and lactobacillus casei composition, the bacterial concentration ratio of described bacillus cereus, bacillus subtilis and lactobacillus casei is
1:1～3:1～2.5.
A kind of compound micro-ecological preparation the most according to claim 1, it is characterised in that described bacillus cereus, hay
The bacterial concentration ratio of bacillus cereus and lactobacillus casei is 1:3:2.
A kind of compound micro-ecological preparation the most according to claim 1, it is characterised in that described bacillus cereus and hay
Bacillus cereus screens from cecal content and obtains.
A kind of compound micro-ecological preparation the most according to claim 3, it is characterised in that described bacillus cereus and hay
Bacillus cereus screens from night blindness intestinal contents and obtains.
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| CN104250630A (en) * | 2014-09-19 | 2014-12-31 | 安徽农业大学 | Preparation method of solid probiotics preparation |
| CN104286419A (en) * | 2014-10-24 | 2015-01-21 | 沈阳天佳华生物科技有限公司 | Novel feed additive |
| CN104686838A (en) * | 2015-02-11 | 2015-06-10 | 青岛根源生物技术集团有限公司 | Composite microecological feed additive capable of increasing freshwater fish growth rate and application of composite microecological feed additive |
| CN105287651B (en) * | 2015-10-20 | 2019-03-08 | 安徽农业大学 | The application of the preparation method of feeding type probiotics and the feeding type probiotics |
| CN107299071A (en) * | 2017-08-16 | 2017-10-27 | 济南青瑞生物科技有限公司 | A kind of enteric probiotics preparation |
| CN109133368A (en) * | 2018-09-13 | 2019-01-04 | 无锡跃洋生物科技有限公司 | A kind of complex microorganism water purification agent and preparation method thereof for aquaculture |
| CN110144307B (en) * | 2019-05-13 | 2020-12-22 | 华中农业大学 | Lactobacillus gasseri for resisting enterohemorrhagic escherichia coli, prepared poultry composite probiotic preparation and application |
| CN114891690B (en) * | 2022-06-08 | 2023-09-08 | 山东仙普爱瑞科技股份有限公司 | Composite microbial inoculant with growth promoting performance and application thereof |
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| 张璐.饲用芽孢杆菌产品益生特性的体外评价研究.《中国优秀硕士学位论文全文数据库 农业科技辑》.2008,(第10期),D050-22. * |
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