CN103652367B - A kind of ruminant composite microorganism type forage additives and preparation method thereof - Google Patents
A kind of ruminant composite microorganism type forage additives and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of ruminant complex microecological feed and preparation method thereof.By microbial forage additive and fulvic acid composite according to 2.5-5.0: 1; Described microbial forage additive is; Solid medium containing sodium humate is prepared from through plastc ring fermentation.Product of the present invention, as additive, has probio and fulvic acid two kinds of effects, can not only improve ruminant micro ecology of gastrointestinal tract balance, increase feed intake, strengthen animal body immunity; Can improve food conversion ratio simultaneously, improve the price of deed, reduce production cost.
Description
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof, particularly a kind of ruminant composite microorganism type forage additives and preparation method thereof.
Background technology
Along with the continuous growth of the size of population and improving constantly of people's material progress living standard, people to the demand of livestock products also in continuous increase.Meanwhile, the quality safety of people to livestock products there has also been higher requirement.Common demands and the demand having become people such as pollution-free, green, safe of animal product.Current feed addictive is mainly the nutriment additive containing compositions such as mineral matter, vitamin, amino acid; Antibiotic agents, in order to prevent disease; The fragrance conditioning agents such as all kinds of essence, in order to strengthen the palatability of feed; Steroids additive, in order to speed the mushroom conditioning agent of animal.But above-mentioned four kinds of products are except price is higher, the health of the problem serious harm mankind such as the medicament residue caused, the resistance to the action of a drug.
Summary of the invention
The object of this invention is to provide a kind of ruminant composite microorganism type forage additives and preparation method thereof, from the physilogical characteristics of ruminant, ruminant compound micro-ecological preparation is studied with the viewpoint of cud microecology and system animal nutrition, emphasize that the position emphasis of probiotics effect is at cud, instead of at small intestine as nonruminant.On the other hand, the action effect of probiotics to ruminant depends on bacterial classification classification used and science combination thereof, preparation vigor, application dose, simultaneously also relevant with the factor such as the physiological status residing for animal, Diet Types, at present the systematic Study for above factor is lacked to the research of Ruminat s Microecological Agent.Therefore, the present invention adopts modern biotechnology to ferment and compounded technology, sodium humate is added in culture medium the probiotics prepared containing humic acid, then probiotics and fulvic acid are pressed proper proportion composite, prepare the composite microorganism type forage additives of applicable ruminant, this product is the probiotics that a class contains humic acid substance, compared with single probiotics, promotion cattle and sheep, feed digestion is absorbed, improve cud micro-ecological environment, improve production performance, strengthen immunity of organisms, the action effect reducing the aspects such as environmental pollution is better.
For achieving the above object, the object of the invention is to the invention provides a kind of ruminant composite microorganism type forage additives, by microbial forage additive and fulvic acid composite according to 2.5-5.0: 1; Described microbial forage additive is: be prepared from through plastc ring fermentation by the solid medium containing sodium humate.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 2-5: 1.5-3.5: 0.5-2 ratio; Plastc ring is being 8-10% (W/V) containing the inoculative proportion in the solid medium of sodium humate; The described solid medium containing sodium humate is made up of solid matter and water, its ratio is 1: 0.8-1.1, and wherein solid matter constituent comprises: wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05%.
It is 1.0 × 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (BacillussubtilisCGMCC1.0892) viable count
8-9.9 × 10
8cfu/g, S. cervisiae CGMCC2.119 (SaccharomycescerevisiaeCGMCC2.119) viable count are 1.0 × 10
8-9.9 × 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (LactobacillusplantarumCGMCC1.0557) viable count are 1.0 × 10
9-9.9 × 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) cultivated through inclined-plane successively by bacillus subtilis, primary seed solution is cultivated, secondary seed solution is cultivated, the seed liquor of obtained bacillus subtilis viable bacteria, viable count reaches 1.0 × 10
8-9.9 × 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40-55g/L, peptone 8-11g/L, beef extract 25-35g/L, glucose 45-55g/L, MgCl
20.3-1.1g/L, K
2hPO
40.05-0.15g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of bacillus subtilis is 32-36 DEG C, and the time is 22-26 hour;
(2) cultivated through inclined-plane successively by S. cervisiae, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained S. cervisiae, viable count reaches 1.0 × 10
8-9.9 × 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13-17g/L, glucose 7-12g/L, MgSO
40.1-0.3g/L, K
2hPO
40.3-0.6g/L, (NH
4)
2sO
40.3-0.6g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of saccharomyces cerevisiae is 32-36 DEG C, and the time is 22-26 hour;
(3) cultivated through inclined-plane by Lactobacillus plantarum, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained Lactobacillus plantarum, viable count reaches 1.0 × 10
9-9.9 × 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 8-12g/L, wheat bran 7-12g/L, glucose 3-7g/L, MgCl
20.05-0.15g/L, K
2hPO
40.5-0.9g/L, (NH
4)
2sO
40.3-0.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35-38 DEG C, the time is 10-15 hour;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 2-5: 1.5-3.5: 0.5-2 ratio, be prepared into plastc ring;
(5) by wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05% mixing (pressing mass percent respectively), the solid matter in culture medium is prepared into.
(6) above-mentioned (5) gained solid matter is mixed in 1: 0.8-1.1 ratio with water, obtain the solid medium containing sodium humate;
(7) be inoculated in the solid medium of above-mentioned (6) gained by above-mentioned (4) gained plastc ring, its ratio is 8-10% (W/V); At 30-40 DEG C of condition bottom fermentation 36-48 hour, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid are carried out according to 2.5-5.0: 1 composite, get product.
The invention has the advantages that:
(1) sodium humate is added in solid medium by the present invention, facilitates the growth and breeding of probio, is conducive to the generation of tunning;
(2) the present invention by micro-ecological feed and fulvic acid composite with proper proportion, micro-ecological feed is made to play better effects if in animal body, there is humic acid substance to effects such as the growth promotion of animal and raising immunity simultaneously, can be used as the antibiotic substitute products of a class;
(3) in actual production, use product of the present invention can improve milk production of cow 10% ~ 25%, improve mutton sheep daily gain 13% ~ 20%, reduce mutton sheep feed-weight ratio 10 ~ 25%.
From the physilogical characteristics of ruminant, study ruminant compound micro-ecological preparation with the viewpoint of cud microecology and system animal nutrition, emphasize that the position emphasis of probiotics effect is at cud, instead of at small intestine as nonruminant.On the other hand, the action effect of probiotics to ruminant depends on bacterial classification classification used and science combination thereof, preparation vigor, application dose, simultaneously also relevant with the factor such as the physiological status residing for animal, Diet Types, at present the systematic Study for above factor is lacked to the research of Ruminat s Microecological Agent.Therefore, the present invention adopts modern biotechnology to ferment and compounded technology, sodium humate is added in culture medium the probiotics prepared containing humic acid, then probiotics and fulvic acid are pressed proper proportion composite, prepare the composite microorganism type forage additives of applicable ruminant, this product is the probiotics that a class contains humic acid substance, compared with single probiotics, promotion cattle and sheep, feed digestion is absorbed, improve cud micro-ecological environment, improve production performance, strengthen immunity of organisms, the action effect reducing the aspects such as environmental pollution is better.
Detailed description of the invention
Embodiment 1
A kind of ruminant composite microorganism type forage additives, by microbial forage additive and fulvic acid composite according to 3.5: 1; Described microbial forage additive is: be prepared from through plastc ring fermentation by the solid medium containing sodium humate.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 3: 2.5: 1 ratios; Plastc ring is being 8% (W/V) containing the inoculative proportion in the solid medium of sodium humate; The described solid medium containing sodium humate is made up of solid matter and water, its ratio is 1: 1, and wherein solid matter constituent comprises: wheat bran 45%, dregs of beans 28%, maize peel 20%, sodium humate 5%, ammonium sulfate 1%, dipotassium hydrogen phosphate 0.9%, magnesium sulfate 0.05% and zinc chloride 0.05%.
It is 8 × 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (BacillussubtilisCGMCC1.0892) viable count
8cfu/g, S. cervisiae CGMCC2.119 (SaccharomycescerevisiaeCGMCC2.119) viable count are 8 × 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (LactobacillusplantarumCGMCC1.0557) viable count are 8 × 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) cultivated through inclined-plane successively by bacillus subtilis, primary seed solution is cultivated, secondary seed solution is cultivated, the seed liquor of obtained bacillus subtilis viable bacteria, viable count reaches 8 × 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 45g/L, peptone 9g/L, beef extract 30g/L, glucose 45g/L, MgCl
21g/L, K
2hPO
40.1g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of bacillus subtilis is 33 DEG C, and the time is 24 hours;
(2) cultivated through inclined-plane successively by S. cervisiae, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained S. cervisiae, viable count reaches 8 × 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 15g/L, glucose 9g/L, MgSO
40.2g/L, K
2hPO
40.5g/L, (NH
4)
2sO
40.5g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of saccharomyces cerevisiae is 33 DEG C, and the time is 24 hours;
(3) cultivated through inclined-plane by Lactobacillus plantarum, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained Lactobacillus plantarum, viable count reaches 8 × 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 10g/L, wheat bran 8g/L, glucose 5g/L, MgCl
20.1g/L, K
2hPO
40.7g/L, (NH
4)
2sO
40.5g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35 DEG C, the time is 12 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 3: 2.5: 1 ratios, be prepared into plastc ring;
(5) wheat bran 45%, dregs of beans 28%, maize peel 20%, sodium humate 5%, ammonium sulfate 1%, dipotassium hydrogen phosphate 0.9%, magnesium sulfate 0.05% and zinc chloride 0.05% is mixed (respectively by mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 1 ratio with water, obtain the solid medium containing sodium humate;
(7) be inoculated in the solid medium of above-mentioned (6) gained by above-mentioned (4) gained plastc ring, its ratio is 8%W/V); 35 DEG C of condition bottom fermentations 40 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid are carried out according to 3.5: 1 composite, get product.
Embodiment 2
A kind of ruminant composite microorganism type forage additives, by microbial forage additive and fulvic acid composite according to 4.5: 1; Described microbial forage additive is: be prepared from through plastc ring fermentation by the solid medium containing sodium humate.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 4: 3: 1.5 ratios; Plastc ring is being 9% (W/V) containing the inoculative proportion in the solid medium of sodium humate; The described solid medium containing sodium humate is made up of solid matter and water, its ratio is 1: 0.8, and wherein solid matter constituent comprises: wheat bran 40%, dregs of beans 25%, maize peel 30%, sodium humate 3%, ammonium sulfate 0.9%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1%.
Described fulvic acid extracts the brown coal that raw material is area, Wuchuan, Inner Mongolia Autonomous Region; Described sodium humate extracts the brown coal that raw material is area, Wuchuan, Inner Mongolia Autonomous Region.
Described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (BacillussubtilisCGMCC1.0892) 9 × 10
8cfu/g, S. cervisiae CGMCC2.119 (SaccharomycescerevisiaeCGMCC2.119) 9 × 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (LactobacillusplantarumCGMCC1.0557) 9 × 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) cultivated through inclined-plane successively by bacillus subtilis, primary seed solution is cultivated, secondary seed solution is cultivated, the seed liquor of obtained bacillus subtilis viable bacteria, viable count reaches 9 × 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40g/L, peptone 10g/L, beef extract 25g/L, glucose 50g/L, MgCl
20.7g/L, K
2hPO
40.09g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of bacillus subtilis is 35 DEG C, and the time is 22 hours;
(2) cultivated through inclined-plane successively by S. cervisiae, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained S. cervisiae, viable count reaches 9 × 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13g/L, glucose 11g/L, MgSO
40.3g/L, K
2hPO
40.6g/L, (NH
4)
2sO
40.4g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of saccharomyces cerevisiae is 35 DEG C, and the time is 22 hours;
(3) cultivated through inclined-plane by Lactobacillus plantarum, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained Lactobacillus plantarum, viable count reaches 9 × 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 9g/L, wheat bran 10g/L, glucose 6g/L, MgCl
20.15g/L, K
2hPO
40.5g/L, (NH
4)
2sO
40.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 37 DEG C, the time is 10 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 4: 3: 1.5 ratios, be prepared into plastc ring;
(5) wheat bran 40%, dregs of beans 25%, maize peel 30%, sodium humate 3%, ammonium sulfate 0.9%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1% are mixed (respectively by mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 0.8 ratio with water, obtain the solid medium containing sodium humate;
(7) be inoculated in the solid medium of above-mentioned (6) gained by above-mentioned (4) gained plastc ring, its ratio is 9% (W/V); 30 DEG C of condition bottom fermentations 47 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid are carried out according to 4.5: 1 composite, get product.
Embodiment 3
A kind of ruminant composite microorganism type forage additives, by microbial forage additive and fulvic acid composite according to 3.0: 1; Described microbial forage additive is: be prepared from through plastc ring fermentation by the solid medium containing sodium humate.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 5: 3: 2 ratios; Plastc ring is being 10% (W/V) containing the inoculative proportion in the solid medium of sodium humate; The described solid medium containing sodium humate is made up of solid matter and water, its ratio is 1: 1.1, and wherein solid matter constituent comprises: wheat bran 43%, dregs of beans 27%, maize peel 22.6%, sodium humate 6%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.02% and zinc chloride 0.03%.
It is 6 × 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (BacillussubtilisCGMCC1.0892) viable count
8cfu/g, S. cervisiae CGMCC2.119 (SaccharomycescerevisiaeCGMCC2.119) viable count are 6 × 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (LactobacillusplantarumCGMCC1.0557) viable count are 6 × 10
8cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) cultivated through inclined-plane successively by bacillus subtilis, primary seed solution is cultivated, secondary seed solution is cultivated, the seed liquor of obtained bacillus subtilis viable bacteria, viable count reaches 6 × 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 50g/L, peptone 8g/L, beef extract 35g/L, glucose 55g/L, MgCl
20.3g/L, K
2hPO
40.15g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of bacillus subtilis is 32 DEG C, and the time is 26 hours;
(2) cultivated through inclined-plane successively by S. cervisiae, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained S. cervisiae, viable count reaches 6 × 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 17g/L, glucose 7g/L, MgSO
40.1g/L, K
2hPO
40.3g/L, (NH
4)
2sO
40.6g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of saccharomyces cerevisiae is 32 DEG C, and the time is 26 hours;
(3) cultivated through inclined-plane by Lactobacillus plantarum, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained Lactobacillus plantarum, viable count reaches 6 × 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 12g/L, wheat bran 7g/L, glucose 3g/L, MgCl
20.05g/L, K
2hPO
40.9g/L, (NH
4)
2sO
40.3g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 36 DEG C, the time is 13 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 5: 3: 2 ratios, be prepared into plastc ring;
(5) wheat bran 43%, dregs of beans 27%, maize peel 22.6%, sodium humate 6%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.02% and zinc chloride 0.03% is mixed (respectively by mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 1.1 ratio with water, obtain the solid medium containing sodium humate;
(7) be inoculated in the solid medium of above-mentioned (6) gained by above-mentioned (4) gained plastc ring, its ratio is 10% (W/V); 37 DEG C of condition bottom fermentations 36 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid are carried out according to 3.0: 1 composite, get product.
Following embodiment is done below with regard to animal:
Embodiment 1
Mutton sheep is tested
Test select same kind, same to sex, body weight (19.4kg ± 1.24) close, healthy anosis 4 the monthly age lamb 120, be divided into 4 groups at random, comprise control group and test I ~ III group, often organize 30, group difference is not significantly (P > 0.05).Test group adds ruminant composite microorganism type forage additives 1%.Fine fodder Diet Formula and trophic level are tested the smart coarse fodder supply of sheep, feeding method, control measures and daily arrangement in table 1. and are all undertaken by sheep field conventional method.Grouping is raised, and drinking-water, illumination, motion etc. are consistent.Control group is identical with the trophic level of test group.On-test and off-test are carried out 2 empty stomaches and are weighed, and each continuous weighting 2d, averages, and measure daily gain.And record inventory, calculate the average day consumption of each group, calculate feed conversion rate according to weightening finish.Meanwhile, also without exception etc. observation is had to the defecation number of times of sheep, excrement amount and shape.Test totally 70 days, within first 10 days, in order to raise the phase in advance, the formal phase is 60 days.
Table 1 mutton sheep mixed concentrate composition and trophic level (air bells dry basis)
Ruminant composite microorganism type forage additives is on the daily gain of mutton sheep and the impact of feed-weight ratio in table 2, and as seen from the table, test group comparatively control group daily gain and feed conversion rate is improved, and test daily gain improves 13.72%, 17.11% and 19.08% respectively; Test group feed conversion rate comparatively control group improves 17.44%, 19.26% and 15.45%.Complex microecological feed all increases significantly to mutton sheep daily gain and feed conversion rate thus.
The change of table 2 mutton sheep daily gain and feed-weight ratio
Embodiment 2:
Milk cow is tested:
Select healthy, normal, body weight, parity, the output of milk of growing and the approximate china holstein cows 80 of calving number of days, RANDOMIZED BLOCK DESIGN is adopted to be divided into 4 groups (1 control group and 3 test group), control group, test group adds compound micro-ecological preparation 1%.Fine fodder Diet Formula is corn 54%, wheat bran 13%, dregs of beans 15%, cotton dregs 13%, stone flour 1%, calcium monohydrogen phosphate 1%, salt 1%, sodium bicarbonate 1%, premix 1%.Test the smart coarse fodder supply of milk cow, feeding method, control measures and daily arrangement all to be undertaken by cattle farm conventional method.Test milk cow is milked for 2 times every day.Free choice feeding and drinking-water, furnish good supplies to clean drinking-water, keeps colony house health.
Table 3 ruminant compound microecological preparation is on the impact of milk cow production performance
Result of the test is in table 3, as seen from the table, each test group comparatively control group milk yield, lactoprotein is improved, and somatic number declines to some extent, the situation of change of the comprehensive output of milk and milk-quality, the output of milk of test group to milk cow is significantly improved, test I improves 19.84%, and test II improves 13.05%, and test III improves 14.91%, as can be seen here, feeding ruminants compound micro-ecological preparation can improve the output of milk more than 10.
In sum, the feature of this product is:
(1) sodium humate is added in solid medium by the present invention, facilitates the growth and breeding of probio, is conducive to the generation of tunning;
(2) the present invention by micro-ecological feed and fulvic acid composite with proper proportion, micro-ecological feed is made to play better effects if in animal body, there is humic acid substance to effects such as the growth promotion of animal and raising immunity simultaneously, can be used as the antibiotic substitute products of a class;
(3) in actual production, use product of the present invention can improve milk production of cow 10% ~ 25%, improve mutton sheep daily gain 13% ~ 20%, reduce mutton sheep feed-weight ratio 10 ~ 25%.
It should be noted last that, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although elaborate invention has been with reference to embodiment, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not depart from the spirit and scope of technical solution of the present invention.
Claims (1)
1. a ruminant composite microorganism type forage additives, its preparation method is:
(1) cultivated through inclined-plane successively by bacillus subtilis, primary seed solution is cultivated, secondary seed solution is cultivated, the seed liquor of obtained bacillus subtilis viable bacteria, viable count reaches 1.0 × 10
8-9.9 × 10
8cfu/g; The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40-55g/L, peptone 8-11g/L, beef extract 25-35g/L, glucose 45-55g/L, MgCl
20.3-1.1g/L, K
2hPO
40.05-0.15g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of bacillus subtilis is 32-36 DEG C, and the time is 22-26 hour;
(2) cultivated through inclined-plane successively by S. cervisiae, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained S. cervisiae, viable count reaches 1.0 × 10
8-9.9 × 10
8cfu/g; The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13-17g/L, glucose 7-12g/L, MgSO
40.1-0.3g/L, K
2hPO
40.3-0.6g/L, (NH
4)
2sO
40.3-0.6g/L; The cultivation temperature that inclined-plane is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated of saccharomyces cerevisiae is 32-36 DEG C, and the time is 22-26 hour;
(3) cultivated through inclined-plane by Lactobacillus plantarum, primary seed solution is cultivated and secondary seed solution is cultivated, the seed liquor of obtained Lactobacillus plantarum, viable count reaches 1.0 × 10
9-9.9 × 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 8-12g/L, wheat bran 7-12g/L, glucose 3-7g/L, MgCl
20.05-0.15g/L, K
2hPO
40.5-0.9g/L, (NH
4)
2sO
40.3-0.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35-38 DEG C, the time is 10-15 hour;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 2-5: 1.5-3.5: 0.5-2 ratio, be prepared into plastc ring;
(5) by wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05% mixing (pressing mass percent respectively), the solid matter in culture medium is prepared into;
(6) above-mentioned (5) gained solid matter is mixed in 1: 0.8-1.1 (W/V) ratio with water, obtain the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio is 8-10% (V/W), at 30-40 DEG C of condition bottom fermentation 36-48 hour, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid are carried out according to 2.5-5.0: 1 composite, get product.
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| CN105614085B (en) * | 2016-01-12 | 2020-12-08 | 内蒙古农业大学 | Preparation method of high-activity cow fermented concentrated feed and special composite microbial agent thereof |
| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
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| CN107594149A (en) * | 2017-09-29 | 2018-01-19 | 内蒙古凯嘉生物技术有限责任公司 | A kind of ecological fermentation feed and its preparation technology |
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| CN109851450A (en) * | 2019-01-30 | 2019-06-07 | 叶荫人 | A kind of microbial degradation method of coal waste and application |
| CN114908026A (en) * | 2022-06-30 | 2022-08-16 | 哈尔滨韶创生物科技有限公司 | Special liquid composite microecological preparation for rumination and preparation method thereof |
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