CN103652367A - Composite micro-ecological feed additive for ruminants and preparation method thereof - Google Patents
Composite micro-ecological feed additive for ruminants and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a composite micro-ecological feed additive for ruminants and a preparation method thereof. A micro-ecological feed additive and fulvic acid are compounded according to the ratio of (2.5-5.0):1; and the micro-ecological feed additive is prepared by mixing solid culture medium containing sodium humate and bacterium suspension and then carrying out fermentation. As an additive, the composite micro-ecological feed has the two effects of probiotics and the fulvic acid, so that not only can the micro-ecological balance of the gastrointestinal tract of the ruminants be improved, the food intake be increased, the immunity of the organisms of the ruminants be enhanced, but also the feed conversion rate and the feed reward can be improved and the production cost can be reduced simultaneously.
Description
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof, particularly a kind of ruminant composite microorganism type forage additives and preparation method thereof.
Background technology
Along with the continuous growth of the size of population and improving constantly of people's material progress living standard, people to the demand of livestock products also in continuous increase.Meanwhile, people have also had higher requirement to the quality safety of livestock products.Generally requirement and the demand that has become people such as pollution-free, green, safe of animal product.Feed addictive is mainly the nutriment additive containing compositions such as mineral matter, vitamin, amino acid at present; Antibiotics material, in order to prevent disease; The fragrance conditioning agents such as all kinds of essence, in order to strengthen the palatability of feed; Steroids additive, in order to speed the mushroom conditioning agent of animal.But above-mentioned four kinds of products are except price is higher, the problem serious harm mankind's such as the medicament residue causing, the resistance to the action of a drug health.
Summary of the invention
The object of this invention is to provide a kind of ruminant composite microorganism type forage additives and preparation method thereof, physilogical characteristics from ruminant, viewpoint with cud microecology and system animal nutrition is studied ruminant compound micro-ecological preparation, emphasize that the position emphasis of probiotics effect is at cud, rather than as nonruminant at small intestine.On the other hand, probiotics depends on bacterial classification classification used and science combination thereof, preparation vigor, application dose to the action effect of ruminant, simultaneously also relevant with the factor such as the residing physiological status of animal, Diet Types, at present the research of Ruminat s Microecological Agent is lacked to the systematic Study for above factor.Therefore, the present invention adopts modern biofermentation and compounded technology, sodium humate is added into and in culture medium, prepares the probiotics containing humic acid, then probiotics and fulvic acid is composite by proper proportion, prepared the composite microorganism type forage additives of applicable ruminant, this product is the probiotics that a class contains humic acid substance, compare with single probiotics, promoting that cattle and sheep digest and assimilate feed, improve cud micro-ecological environment, improve production performance, strengthen immunity of organisms, the action effect that reduces the aspects such as environmental pollution is better.
For achieving the above object, the object of the invention is the invention provides a kind of ruminant composite microorganism type forage additives, by microbial forage additive and fulvic acid according to 2.5-5.0: 1 is composite; Described microbial forage additive is: by the solid medium containing sodium humate, through plastc ring, fermentation is prepared from.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor are according to 2-5: 1.5-3.5: 0.5-2 ratio mixes; Plastc ring is 8-10% (W/V) at the inoculative proportion containing in the solid medium of sodium humate; The described solid medium containing sodium humate is comprised of solid matter and water, its ratio is 1: 0.8-1.1, wherein solid matter constituent comprises: wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05%.
It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described fulvic acid extracts raw material; It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described sodium humate extracts raw material.
It is 1.0 * 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (Bacillus subtilis CGMCC1.0892) viable count
8-9.9 * 10
8cfu/g, S. cervisiae CGMCC2.119 (Saccharomyces cerevisiae CGMCC2.119) viable count is 1.0 * 10
8-9.9 * 108cfu/g, Lactobacillus plantarum CGMCC1.0557 (Lactobacillus plantarum CGMCC1.0557) viable count is 1.0 * 10
9-9.9 * 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) bacillus subtilis is cultivated through inclined-plane cultivation, primary seed solution cultivation, secondary seed solution successively, made the seed liquor of bacillus subtilis viable bacteria, viable count reaches 1.0 * 10
8-9.9 * 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40-55g/L, peptone 8-11g/L, beef extract 25-35g/L, glucose 45-55g/L, MgCl
20.3-1.1g/L, K
2hPO
40.05-0.15g/L; The cultivation temperature that the inclined-plane of bacillus subtilis is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32-36 ℃, and the time is 22-26 hour;
(2) S. cervisiae is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution successively, made the seed liquor of S. cervisiae, viable count reaches 1.0 * 10
8-9.9 * 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13-17g/L, glucose 7-12g/L, MgSO
40.1-0.3g/L, K
2hPO
40.3-0.6g/L, (NH
4)
2sO
40.3-0.6g/L; The cultivation temperature that the inclined-plane of saccharomyces cerevisiae is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32-36 ℃, and the time is 22-26 hour;
(3) Lactobacillus plantarum is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution, made the seed liquor of Lactobacillus plantarum, viable count reaches 1.0 * 10
9-9.9 * 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 8-12g/L, wheat bran 7-12g/L, glucose 3-7g/L, MgCl
20.05-0.15g/L, K
2hPO
40.5-0.9g/L, (NH
4)
2sO
40.3-0.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35-38 ℃, time and is 10-15 hour;
(4) by above-mentioned (1), (2), (3) gained seed liquor in 2-5: 1.5-3.5: 0.5-2 ratio is mixed, and is prepared into plastc ring;
(5) wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05% are mixed to (pressing respectively mass percent), be prepared into the solid matter in culture medium.
(6) by above-mentioned (5) gained solid matter with water in 1: 0.8-1.1 ratio is mixed, and obtains the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio is 8-10% (W/V); At 30-40 ℃ of condition bottom fermentation 36-48 hour, obtain microbial forage additive;
(8) by the microbial forage additive of above-mentioned (7) gained and fulvic acid according to 2.5-5.0: 1 carries out compositely, gets product.
The invention has the advantages that:
(1) the present invention is added into sodium humate in solid medium, has promoted the growth and breeding of probio, is conducive to the generation of tunning;
(2) the present invention is composite with proper proportion by micro-ecological feed and fulvic acid, make micro-ecological feed bring into play in animal body better effects if, there is humic acid substance to the growth promotion of animal simultaneously and improve the effects such as immunity, can be used as the antibiotic substitute products of a class;
(3) in actual production, use product of the present invention can improve milk production of cow 10%～25%, improve mutton sheep daily gain 13%～20%, reduce mutton sheep material anharmonic ratio 10～25%.
From the physilogical characteristics of ruminant, with the viewpoint of cud microecology and system animal nutrition, study ruminant compound micro-ecological preparation, emphasize that the position emphasis of probiotics effect is at cud, rather than as nonruminant at small intestine.On the other hand, probiotics depends on bacterial classification classification used and science combination thereof, preparation vigor, application dose to the action effect of ruminant, simultaneously also relevant with the factor such as the residing physiological status of animal, Diet Types, at present the research of Ruminat s Microecological Agent is lacked to the systematic Study for above factor.Therefore, the present invention adopts modern biofermentation and compounded technology, sodium humate is added into and in culture medium, prepares the probiotics containing humic acid, then probiotics and fulvic acid is composite by proper proportion, prepared the composite microorganism type forage additives of applicable ruminant, this product is the probiotics that a class contains humic acid substance, compare with single probiotics, promoting that cattle and sheep digest and assimilate feed, improve cud micro-ecological environment, improve production performance, strengthen immunity of organisms, the action effect that reduces the aspects such as environmental pollution is better.
The specific embodiment
Embodiment 1
A ruminant composite microorganism type forage additives, microbial forage additive and fulvic acid is composite according to 3.5: 1; Described microbial forage additive is: by the solid medium containing sodium humate, through plastc ring, fermentation is prepared from.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 3: 2.5: 1 ratios; Plastc ring is 8% (W/V) at the inoculative proportion containing in the solid medium of sodium humate; The described solid medium containing sodium humate is comprised of solid matter and water, its ratio is 1: 1, and wherein solid matter constituent comprises: wheat bran 45%, dregs of beans 28%, maize peel 20%, sodium humate 5%, ammonium sulfate 1%, dipotassium hydrogen phosphate 0.9%, magnesium sulfate 0.05% and zinc chloride 0.05%.
It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described fulvic acid extracts raw material; It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described sodium humate extracts raw material.
It is 8 * 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (Bacillus subtilis CGMCC1.0892) viable count
8cfu/g, S. cervisiae CGMCC2.119 (Saccharomyces cerevisiae CGMCC2.119) viable count is 8 * 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (Lactobacillus plantarum CGMCC1.0557) viable count is 8 * 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) bacillus subtilis is cultivated through inclined-plane cultivation, primary seed solution cultivation, secondary seed solution successively, made the seed liquor of bacillus subtilis viable bacteria, viable count reaches 8 * 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 45g/L, peptone 9g/L, beef extract 30g/L, glucose 45g/L, MgCl
21g/L, K
2hPO
40.1g/L; The cultivation temperature that the inclined-plane of bacillus subtilis is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 33 ℃, and the time is 24 hours;
(2) S. cervisiae is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution successively, made the seed liquor of S. cervisiae, viable count reaches 8 * 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 15g/L, glucose 9g/L, MgSO
40.2g/L, K
2hPO
40.5g/L, (NH
4)
2sO
40.5g/L, the cultivation temperature that the inclined-plane of saccharomyces cerevisiae is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 33 ℃, and the time is 24 hours;
(3) Lactobacillus plantarum is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution, made the seed liquor of Lactobacillus plantarum, viable count reaches 8 * 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 10g/L, wheat bran 8g/L, glucose 5g/L, MgCl
20.1g/L, K
2hPO
40.7g/L, (N14
4)
2sO
40.5g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35 ℃, time and is 12 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 3: 2.5: 1 ratios, be prepared into plastc ring;
(5) wheat bran 45%, dregs of beans 28%, maize peel 20%, sodium humate 5%, ammonium sulfate 1%, dipotassium hydrogen phosphate 0.9%, magnesium sulfate 0.05% and zinc chloride 0.05% are mixed to (pressing respectively mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 1 ratio with water, obtain the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio is 8%W/V); 35 ℃ of condition bottom fermentations 40 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid were carried out composite according to 3.5: 1, get product.
Embodiment 2
A ruminant composite microorganism type forage additives, microbial forage additive and fulvic acid is composite according to 4.5: 1; Described microbial forage additive is: by the solid medium containing sodium humate, through plastc ring, fermentation is prepared from.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 4: 3: 1.5 ratios; Plastc ring is 9% (W/V) at the inoculative proportion containing in the solid medium of sodium humate; The described solid medium containing sodium humate is comprised of solid matter and water, its ratio is 1: 0.8, and wherein solid matter constituent comprises: wheat bran 40%, dregs of beans 25%, maize peel 30%, sodium humate 3%, ammonium sulfate 0.9%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1%.
It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described fulvic acid extracts raw material; It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described sodium humate extracts raw material.
Described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (Bacillus subtilis CGMCC1.0892) 9 * 10
8cfu/g, S. cervisiae CGMCC2.119 (Saccharomyces cerevisiae CGMCC2.119) 9 * 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (Lactobacillus plantarum CGMCC1.0557) 9 * 10
9cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) bacillus subtilis is cultivated through inclined-plane cultivation, primary seed solution cultivation, secondary seed solution successively, made the seed liquor of bacillus subtilis viable bacteria, viable count reaches 9 * 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40g/L, peptone 10g/L, beef extract 25g/L, glucose 50g/L, MgCl
20.7g/L, K
2hPO
40.09g/L; The cultivation temperature that the inclined-plane of bacillus subtilis is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35 ℃, and the time is 22 hours;
(2) S. cervisiae is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution successively, made the seed liquor of S. cervisiae, viable count reaches 9 * 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13g/L, glucose 11g/L, MgSO
40.3g/L, K
2hPO
40.6g/L, (NH
4)
2sO
40.4g/L; The cultivation temperature that the inclined-plane of saccharomyces cerevisiae is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35 ℃, and the time is 22 hours;
(3) Lactobacillus plantarum is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution, made the seed liquor of Lactobacillus plantarum, viable count reaches 9 * 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 9g/L, wheat bran 10g/L, glucose 6g/L, MgCl
20.15g/L, K
2hPO
40.5g/L, (NH
4)
2sO
40.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 37 ℃, time and is 10 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 4: 3: 1.5 ratios, be prepared into plastc ring;
(5) wheat bran 40%, dregs of beans 25%, maize peel 30%, sodium humate 3%, ammonium sulfate 0.9%, dipotassium hydrogen phosphate 1%, magnesium sulfate 0.1% are mixed to (pressing respectively mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 0.8 ratio with water, obtain the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio is 9% (W/V); 30 ℃ of condition bottom fermentations 47 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid were carried out composite according to 4.5: 1, get product.
Embodiment 3
A ruminant composite microorganism type forage additives, microbial forage additive and fulvic acid is composite according to 3.0: 1; Described microbial forage additive is: by the solid medium containing sodium humate, through plastc ring, fermentation is prepared from.
Described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor mix according to 5: 3: 2 ratios; Plastc ring is 10% (W/V) at the inoculative proportion containing in the solid medium of sodium humate; The described solid medium containing sodium humate is comprised of solid matter and water, its ratio is 1: 1.1, and wherein solid matter constituent comprises: wheat bran 43%, dregs of beans 27%, maize peel 22.6%, sodium humate 6%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.02% and zinc chloride 0.03%.
It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described fulvic acid extracts raw material; It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described sodium humate extracts raw material.
It is 6 * 10 that described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (Bacillus subtilis CGMCC1.0892) viable count
8cfu/g, S. cervisiae CGMCC2.119 (Saccharomyces cerevisiae CGMCC2.119) viable count is 6 * 10
8cfu/g, Lactobacillus plantarum CGMCC1.0557 (Lactobacillusplantarum CGMCC1.0557) viable count is 6 * 10
8cfu/g.
A kind of preparation method of ruminant composite microorganism type forage additives is:
(1) bacillus subtilis is cultivated through inclined-plane cultivation, primary seed solution cultivation, secondary seed solution successively, made the seed liquor of bacillus subtilis viable bacteria, viable count reaches 6 * 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 50g/L, peptone 8g/L, beef extract 35g/L, glucose 55g/L, MgCl
20.3g/L, K
2hPO
40.15g/L; The cultivation temperature that the inclined-plane of bacillus subtilis is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32 ℃, and the time is 26 hours;
(2) S. cervisiae is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution successively, made the seed liquor of S. cervisiae, viable count reaches 6 * 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 17g/L, glucose 7g/L, MgSO
40.1g/L, K
2hPO
40.3g/L, (NH
4)
2sO
40.6g/L; The cultivation temperature that the inclined-plane of saccharomyces cerevisiae is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32 ℃, and the time is 26 hours;
(3) Lactobacillus plantarum is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution, made the seed liquor of Lactobacillus plantarum, viable count reaches 6 * 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 12g/L, wheat bran 7g/L, glucose 3g/L, MgCl
20.05g/L, K
2hPO
40.9g/L, (NH
4)
2sO
40.3g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 36 ℃, time and is 13 hours;
(4) above-mentioned (1), (2), (3) gained seed liquor are mixed in 5: 3: 2 ratios, be prepared into plastc ring;
(5) wheat bran 43%, dregs of beans 27%, maize peel 22.6%, sodium humate 6%, ammonium sulfate 0.6%, dipotassium hydrogen phosphate 0.75%, magnesium sulfate 0.02% and zinc chloride 0.03% are mixed to (pressing respectively mass percent), be prepared into the solid matter in culture medium.
(6) above-mentioned (5) gained solid matter is mixed in 1: 1.1 ratio with water, obtain the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio is 10% (W/V); 37 ℃ of condition bottom fermentations 36 hours, obtain microbial forage additive;
(8) microbial forage additive of above-mentioned (7) gained and fulvic acid were carried out composite according to 3.0: 1, get product.
With regard to animal, be following embodiment below:
Embodiment 1
Mutton sheep experiment
Test select same kind, same sex, body weight (19.4kg ± 1.24) close, healthy anosis 4 the monthly age 120 of lambs, be divided at random 4 groups, comprise control group and test I～III group, 30 every group, group difference is remarkable (P > 0.05) not.Test group is added ruminant composite microorganism type forage additives 1%.Fine fodder Diet Formula and trophic level are all undertaken by sheep field conventional method in Table smart coarse fodder supply, feeding method, control measures and the daily arrangement of 1. test sheep.Grouping is raised, the term harmonizations such as drinking-water, illumination, motion.The trophic level of control group and test group is identical.On-test and off-test are carried out 2 empty stomaches and are weighed, and each continuous weighting 2d, averages, and measures daily gain.And record inventory, and calculate the average day consumption of each group, according to weightening finish, calculate feed conversion rate.Meanwhile, also the defecation number of times of sheep, excrement amount and shape are had or not to abnormal etc. observation.Test totally 70 days, within first 10 days, in order to raise the phase in advance, the formal phase is 60 days.
Table 1 mutton sheep mixed concentrate is fine into and trophic level (air bells dry basis)
Ruminant composite microorganism type forage additives is on the impact of the daily gain of mutton sheep and material anharmonic ratio in Table 2, and as seen from the table, test group is improved compared with control group daily gain and feed conversion rate, and test daily gain improves respectively 13.72%, 17.11% and 19.08%; Test group feed conversion rate improves 17.44%, 19.26% and 15.45% compared with control group.Complex microecological feed all increases significantly to mutton sheep daily gain and feed conversion rate thus.
The variation of table 2 mutton sheep daily gain and material anharmonic ratio
Embodiment 2:
Milk cow experiment:
Select 80 of the approximate china holstein cowses of healthy, normal, the body weight of growing, parity, the output of milk and calving number of days, adopt RANDOMIZED BLOCK DESIGN to be divided into 4 groups (1 control group and 3 test group), control group, test group is added compound micro-ecological preparation 1%.Fine fodder Diet Formula is corn 54%, wheat bran 13%, dregs of beans 15%, cotton dregs 13%, stone flour 1%, calcium monohydrogen phosphate 1%, salt 1%, sodium bicarbonate 1%, premix 1%.Smart coarse fodder supply, feeding method, control measures and the daily arrangement of test milk cow are all undertaken by cattle farm conventional method.Test milk cow is milked for 2 times every day.Free choice feeding and drinking-water, the clean drinking-water that furnishes good supplies to, keeps colony house health.
The impact of table 3 ruminant compound micro-ecological preparation on milk cow production performance
Result of the test is in Table 3, as seen from the table, each test group is improved compared with control group milk yield, lactoprotein, and somatic number declines to some extent, the situation of change of the comprehensive output of milk and milk-quality, test group is significantly improved to the output of milk of milk cow, test I improves 19.84%, and test II improves 13.05%, and test III improves 14.91%, as can be seen here, the ruminant compound micro-ecological preparation of feeding can improve the output of milk more than 10.
In sum, the feature of this product is:
(1) the present invention is added into sodium humate in solid medium, has promoted the growth and breeding of probio, is conducive to the generation of tunning;
(2) the present invention is composite with proper proportion by micro-ecological feed and fulvic acid, make micro-ecological feed bring into play in animal body better effects if, there is humic acid substance to the growth promotion of animal simultaneously and improve the effects such as immunity, can be used as the antibiotic substitute products of a class;
(3) in actual production, use product of the present invention can improve milk production of cow 10%～25%, improve mutton sheep daily gain 13%～20%, reduce mutton sheep material anharmonic ratio 10～25%.
It should be noted last that, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although the present invention is elaborated with reference to embodiment, those of ordinary skill in the art is to be understood that, can modify or be equal to replacement technical scheme of the present invention, and not depart from the spirit and scope of technical solution of the present invention.
Claims (5)
1. a ruminant composite microorganism type forage additives, is characterized in that: by microbial forage additive and fulvic acid according to 2.5-5.0: 1 is composite; Described microbial forage additive is: by the solid medium containing sodium humate, through plastc ring, fermentation is prepared from.
2. a kind of ruminant composite microorganism type forage additives according to claim 1, is characterized in that: described plastc ring is: bacillus subtilis seed liquor, S. cervisiae seed liquor, Lactobacillus plantarum seed liquor are according to 2-5: 1.5-3.5: 0.5-2 ratio mixes; Plastc ring is 8-10% (W/V) at the inoculative proportion containing in the solid medium of sodium humate; The described solid medium containing sodium humate is comprised of solid matter and water, its ratio is 1: 0.8-1.1, wherein solid matter constituent comprises: wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05%.
3. a kind of ruminant composite microorganism type forage additives according to claim 1 and 2, is characterized in that: it is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described fulvic acid extracts raw material; It is the brown coal in area, Wuchuan, Inner Mongolia Autonomous Region that described sodium humate extracts raw material.
4. a kind of ruminant composite microorganism type forage additives according to claim 2, is characterized in that: described bacillus subtilis, S. cervisiae and Lactobacillus plantarum are respectively bacillus subtilis CGMCC1.0892 (Bacillus subtilis CGMCC1.0892), S. cervisiae CGMCC2.119 (Saccharomyces cerevisiae CGMCC2.119), Lactobacillus plantarum CGMCC1.0557 (Lactobacillus plantarum CGMCC1.0557).
5. according to the preparation method of a kind of ruminant composite microorganism type forage additives described in claim 1 or 2 or 3 or 4, be:
(1) bacillus subtilis is cultivated through inclined-plane cultivation, primary seed solution cultivation, secondary seed solution successively, made the seed liquor of bacillus subtilis viable bacteria, viable count reaches 1.0 * 10
8-9.9 * 10
8cfu/g;
The slant medium of bacillus subtilis is: beef extract-peptone agar medium; First order seed liquid culture medium is beef-protein medium; Secondary seed liquid culture medium consists of dregs of beans 40-55g/L, peptone 8-11g/L, beef extract 25-35g/L, glucose 45-55g/L, MgCl
20.3-1.1g/L, K
2hPO
40.05-0.15g/L; The cultivation temperature that the inclined-plane of bacillus subtilis is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32-36 ℃, and the time is 22-26 hour;
(2) S. cervisiae is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution successively, made the seed liquor of S. cervisiae, viable count reaches 1.0 * 10
8-9.9 * 10
8cfu/g;
The slant medium of saccharomyces cerevisiae is: wort agar culture medium; First order seed liquid culture medium is malt extract medium; Secondary seed liquid culture medium consists of wheat bran 13-17g/L, glucose 7-12g/L, MgSO
40.1-0.3g/L, K
2hPO
40.3-0.6g/L, (NH
4)
2sO
40.3-0.6g/L; The cultivation temperature that the inclined-plane of saccharomyces cerevisiae is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 32-36 ℃, and the time is 22-26 hour;
(3) Lactobacillus plantarum is cultivated and secondary seed solution cultivation through inclined-plane cultivation, primary seed solution, made the seed liquor of Lactobacillus plantarum, viable count reaches 1.0 * 10
9-9.9 * 10
9cfu/g;
The slant medium of Lactobacillus plantarum is MRS agar medium; First order seed liquid culture medium is MRS culture medium; Secondary seed liquid culture medium consists of dregs of beans 8-12g/L, wheat bran 7-12g/L, glucose 3-7g/L, MgCl
20.05-0.15g/L, K
2hPO
40.5-0.9g/L, (NH
4)
2sO
40.3-0.7g/L; The cultivation temperature that the inclined-plane of Lactobacillus plantarum is cultivated, primary seed solution is cultivated and secondary seed solution is cultivated is 35-38 ℃, time and is 10-15 hour;
(4) by above-mentioned (1), (2), (3) gained seed liquor in 2-5: 1.5-3.5: 0.5-2 ratio is mixed, and is prepared into plastc ring;
(5) wheat bran 40-50%, dregs of beans 25-30%, maize peel 20-30%, sodium humate 3-6%, ammonium sulfate 0.5-1%, dipotassium hydrogen phosphate 0.5-1%, magnesium sulfate 0-0.1% and zinc chloride 0-0.05% are mixed to (pressing respectively mass percent), be prepared into the solid matter in culture medium.
(6) by above-mentioned (5) gained solid matter with water in 1: 0.8-1.1 (W/V) ratio is mixed, and obtains the solid medium containing sodium humate;
(7) above-mentioned (4) gained plastc ring is inoculated in the solid medium of above-mentioned (6) gained, its ratio be 8-10% (W/V) at 30-40 ℃ of condition bottom fermentation 36-48 hour, obtain microbial forage additive;
(8) by the microbial forage additive of above-mentioned (7) gained and fulvic acid according to 2.5-5.0: 1 carries out compositely, gets product.
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| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
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