Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of resisting stress premix promoting piglet growth and preparation method thereof, play resisting stress effect from intestinal health, oxidative stress, neurological levels three aspects, improve the anti-stress ability of piglet.
The present invention is achieved by the following technical solutions: a kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate 5-6, phenylacetic acid 2-3, benzoic acid 4-6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, GABA 10-15, composite oligosaccharide 100-150, three solid fermentation green tea slag 465-643.
As the further optimization of such scheme, with parts by weight, composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 120, three solid fermentation green tea slags 617.
As the further optimization of such scheme, with parts by weight, composed of the following components: fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, GABA 15, composite oligosaccharide 130, three solid fermentation green tea slags 548.
As the further optimization of such scheme, described composite oligosaccharide is two kinds and the above compound sugar mixed with arbitrary proportion in mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide.
Promote a preparation method for the resisting stress premix of piglet growth, comprise preparation three solid fermentation green tea slags, prepare three solid fermentation green tea slags and comprise the steps:
(1) fermentate comprise fermentation major ingredient and fermentation auxiliary material, described fermentation major ingredient is green tea slag, by the green tea slag of 60-75% with mix with the fermentation auxiliary material of 30-45% after, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, bacillus subtilis and short and small spore bacillus mix with arbitrary proportion, the mixture that step (1) obtains cultivates 3-5 days at 30-37 DEG C, carry out one time fermentation, in one time fermentation incubation every 2-3h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, described mixed fungus comprises candida utili and aspergillus niger, candida utili and aspergillus niger mix with arbitrary proportion, cultivate 3-5 days at 28-35 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5-7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2-3 days at 30-37 DEG C, carry out three fermentations, in three fermented and cultured processes every 4-6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
As the further optimization of such scheme, described fermentation auxiliary material comprises the dish dregs of rice, cotton dregs and wheat bran, its Chinese food dregs of rice, cotton dregs and, the ratio of wheat bran in fermentate is: dish dregs of rice 10-15%, cotton dregs 10-15% and wheat bran 10-15%.
As the further optimization of such scheme, in one time fermentation, the bacterial concentration of described bacillus subtilis is OD
600=0.5-1, the bacterial concentration OD of described short and small spore bacillus
600=0.5-1, and described bacillus subtilis mixes with volume ratio 1:2 ratio with short and small spore bacillus, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml.
As the further optimization of such scheme, in secondary fermentation, described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus.
Compared with the prior art, the beneficial effect of a kind of resisting stress premix promoting piglet growth of the present invention and preparation method thereof is embodied in:
1, of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth comprises acidulant, solid fermentation green tea slag and microorganism formulation.Add in porkling feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth reduces piglet ight soil humidity, improve delactational piglets survival rate, especially to piglet after wean, good growth performance can also be kept.
2, of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth plays effect from intestinal health, oxidative stress, neurological levels three aspects, there is good effect; Add in pig starter feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth is to the daily gain of piglet, and feed conversion rate there is obvious effect of optimization.
3, of the present inventionly a kind ofly promote that piglet growth is urged in the resisting stress premix of piglet growth, the acidulant contained in premix directly can reduce intestinal pH, changes the enteron aisle acid-base value of piglet, suppresses acid nonfast pathogen to breed.
4, a kind of promotion of the present invention contains three solid fermentation green tea slags in the resisting stress premix of piglet growth, wherein, three times solid fermentation green tea slag is formed by fermentation of bacillus subtilis, bacillus subtilis has obvious inhibiting active material by consuming the oxygen in enteron aisle, the competition mechanism participating in enteron aisle adhesion and generation to pathogenic bacteria, both be the growing environment that the intestinal beneficial such as anaerobic type lactic acid bacteria create anoxic, also effectively suppressed the growth of harmful intestinal tract bacteria simultaneously.
5, a kind of promotion of the present invention contains three solid fermentation green tea slags in the resisting stress premix of piglet growth, the active ingredient mainly Tea Polyphenols of three solid fermentation green tea slags, Tea Polyphenols strengthens piglet anti-stress ability from the oxidativestress damage aspect alleviating hot environment generation.
6, a kind of fumaric acid, citric acid, malic acid, sodium Diacetate, sodium butyrate, phenylacetic acid, green tea slag, mixed bacteria etc. promoting to contain in the resisting stress premix of piglet growth of the present invention all meets forage health standard, the Ministry of Agriculture and national standard, harmful effect can not be caused, safety and environmental protection more to the nutritive value of piglet.
Detailed description of the invention
Elaborate to embodiments of the invention below, the embodiment of the present invention is implemented under premised on the technical program, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30, citric acid 40, malic acid 45, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 2, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 100, three solid fermentation green tea slags 465.
Glutamine in embodiment 1, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 1 is mannan-oligosaccharides and oligomeric maltose mixing oligosaccharide, and wherein mannan-oligosaccharides and oligomeric maltose mix with mass ratio 1:1.
Three solid fermentation green tea slags in embodiment 1 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 70% and 10% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1), the bacterial concentration OD of short and small spore bacillus
600=0.5-1), bacillus subtilis and short and small spore bacillus mix with volume ratio 1:2 ratio, the fermentate that step (1) obtains, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100g:1ml, cultivate 3 days at 30 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus, cultivates 3 days at 28 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 5h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 30 DEG C, carry out three fermentations, in three fermented and cultured processes every 4h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 2
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 50, citric acid 60, malic acid 65, sodium Diacetate 5, sodium butyrate 6, phenylacetic acid 3, benzoic acid 6, glutamine 150, yeast selenium 20, astaxanthin 5, GABA 15, composite oligosaccharide 150, three solid fermentation green tea slags 643.
Glutamine in embodiment 2, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 2 is isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:2:2:1.
Three solid fermentation green tea slags in embodiment 2 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 65% and 15% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1), the bacterial concentration OD of short and small spore bacillus
600=0.5-1), bacillus subtilis and short and small spore bacillus mix with volume ratio 1:1 ratio, the fermentate that step (1) obtains, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 105g:1.05ml, cultivate 4 days at 33 DEG C, carry out one time fermentation, in one time fermentation incubation every 2.5h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus, cultivates 3.5 days at 30 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 6h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2.5 days at 34 DEG C, carry out three fermentations, in three fermented and cultured processes every 4.5h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 3
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 120, three solid fermentation green tea slags 617.
Glutamine in embodiment 3, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 3 is isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:3:1.
Three solid fermentation green tea slags in embodiment 3 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 65% and 10% after autoclaving, the cotton dregs of 12% and the wheat bran of 13% being mixed, add fermented bacterium, ferment; Wherein 65% green tea slag, the dish dregs of rice of 10%, the cotton dregs of 12% and 13% wheat bran be mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1), the bacterial concentration OD of short and small spore bacillus
600=0.5-1), and bacillus subtilis mixes with volume ratio 1:2 ratio with short and small spore bacillus, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 120g:1.05ml, cultivate 3 days at 37 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus, cultivates 3 days at 35 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 6h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 37 DEG C, carry out three fermentations, in three fermented and cultured processes every 6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 4
Promote a resisting stress premix for piglet growth, fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, GABA 15, composite oligosaccharide 130, three solid fermentation green tea slags 548.
Glutamine in embodiment 4, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 4 is isomalto-oligosaccharide and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:1.
Three solid fermentation green tea slags in embodiment 4 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 75% and 10% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment; Wherein 65% green tea slag, the dish dregs of rice of 10%, the cotton dregs of 12% and 13% wheat bran be mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1), the bacterial concentration OD of short and small spore bacillus
600=0.5-1), and bacillus subtilis mixes with volume ratio 3:2 ratio with short and small spore bacillus, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 110g:1.03ml, cultivate 4 days at 35 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:3 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus, cultivates 3 days at 28 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 35 DEG C, carry out three fermentations, in three fermented and cultured processes every 6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Experiment 1
Employing single factor test contrasts, the a kind of of interpolation the preferred embodiments of the present invention 3 of the present invention is utilized to promote the pig starter feed of the resisting stress premix of piglet growth, conventional pig starter feed and add antibiotic pig starter feed to feed 21-23 age in days weanling pig, in three groups, often organize 126, raise 35 days, respectively at the body weight weighing every piglet by group by head on an empty stomach in morning on the same day of on-test and end, record pig starter feed consumption every day, for calculating the weightening finish of piglet average body, average daily gain, average feed intake, feed-weight ratio and ight soil humidity.Specifically see table 1.
Table 1: the piglet growth performance table of comparisons 1
Experimental group and control group initial weight, without significant difference, after cycling hot stress process, are added the end weight of the test group of resisting stress premix, weightening finish, feed intake and survival rate and are significantly higher than control group.Heat stress obviously reduces meat wild pheasant production performance, and a kind of 1-2 of the present invention age in week, meat wild pheasant anti-heat stress feed can this negative effect of larger alleviation, had good anti-heat stress effect.
As shown in Table 1, use a kind of resisting stress premix promoting piglet growth of the present invention, wean in latter 35 days, add antibiotic and do not add antibiotic pig starter feed and compare with feeding, use a kind of average daily gain and feed efficiency promoting the resisting stress premix of piglet growth and add that antibiotic pig starter feed comparatively significantly improves early wea-ning piglet of the present invention; And the piglet average daily gain adding antibiotic group is the highest, but adds and of the present inventionly a kind ofly promote that the baby pig feedstuff anharmonic ratio of the resisting stress premix group of piglet growth is minimum.Use in pig starter feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth effectively reduces early wea-ning piglet ight soil humidity, reduce baby pig diarrhea, improve the survival rate of early wea-ning piglet.
Experiment 2
Employing single factor test contrasts, the a kind of of interpolation the preferred embodiments of the present invention 4 of the present invention is utilized to promote the pig starter feed of the resisting stress premix of piglet growth, conventional pig starter feed and add antibiotic pig starter feed to feed 21-23 age in days weanling pig, in three groups, often organize 210, raise 35 days, respectively at the body weight weighing every piglet by group by head on an empty stomach in morning on the same day of on-test and end, record pig starter feed consumption every day, for calculating the weightening finish of piglet average body, average daily gain, average feed intake, feed-weight ratio and ight soil humidity.Specifically see table 1.
Table 2: the piglet growth performance table of comparisons 2
As shown in Table 2, use a kind of resisting stress premix promoting piglet growth of the present invention, wean in latter 35 days, average daily gain is compared control group with feed efficiency and is significantly improved, and the survival rate of weanling pig, up to 99%, reduces early wea-ning piglet ight soil humidity simultaneously, reduce baby pig diarrhea, make piglet growth performance obtain good improvement, feed-weight ratio significantly reduces, and saves feeding cost.
From result, feed the body weight that a kind of resisting stress premix promoting piglet growth of the present invention and preparation method thereof effectively improves meat wild pheasant, reduce its death rate and feedstuff-meat ratio, larger economic benefit can be created.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.