CN105076862A - Anti-stress premix capable of promoting growth of piglets and preparation method of anti-stress premix - Google Patents

Anti-stress premix capable of promoting growth of piglets and preparation method of anti-stress premix Download PDF

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CN105076862A
CN105076862A CN201510578766.2A CN201510578766A CN105076862A CN 105076862 A CN105076862 A CN 105076862A CN 201510578766 A CN201510578766 A CN 201510578766A CN 105076862 A CN105076862 A CN 105076862A
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fermentation
parts
acid
fermentate
green tea
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CN105076862B (en
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胡洪
戴四发
白晰
闻爱友
高儒松
王淑娟
许金根
任清长
赵磊
华金玲
王立克
朱元召
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Shandong Dayi Biotechnology Group Co.,Ltd.
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Anhui University of Science and Technology
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
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    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses an anti-stress premix capable of promoting growth of piglets. The anti-stress premix comprises the following components in parts by weight: 30-50 parts of fumaric acid, 40-60 parts of citric acid, 45-65 parts of malic acid, 3-5 parts of sodium diacetate, 5-6 parts of sodium butyrate, 2-3 parts of phenylacetic acid, 4-6 parts of benzoic acid, 100-150 parts of glutamine, 10-20 parts of selenium yeast, 3-5 parts of astaxanthin, 10-15 parts of gamma-aminobutyric acid, 100-150 parts of composite oligosaccharide and 465-643 parts of green tea leaves which are solidly fermented for three times. Compared with the prior art, the anti-stress premix and the preparation method have the advantages that the anti-stress premix can play an anti-stress efficacy in three aspects of intestinal health, oxidative stress and nerve level, so that the anti-stress capability of the piglets is improved, the humidity of the excrement of early weaned piglets is effectively decreased, the diarrhea of the piglets is reduced, the immunity and the disease resistance of the piglets are enhanced, and the survival rate of the early weaned piglets is increased.

Description

A kind of resisting stress premix promoting piglet growth and preparation method thereof
Technical field
The present invention relates to a kind of piglet premix, in particular a kind of resisting stress premix promoting piglet growth and preparation method thereof.
Background technology
1-3 week after weaned piglet, because self immune system is not yet physically well developed, digestion power is weak, and premunition is poor, the sharply change of feed diet and living environment, easily make its produce stress, cause grice diarrhoea, feed intake decline, growth retardation, serious is even dead, and as there is mixing or scabies secondary infection, case fatality rate can reach more than 26%.After piglet birth, for guaranteeing that it can tide over wean difficulty smoothly, overcome the series of problems after wean, normal health grows, and needs for it creates good living condition, suitable daily ration.
Nutrition stress be cause piglet wean stress one of main factor, thus, adopt the method for nutrition regulation a to alleviate effective way that early weaning piglet stress be promotion health, efficient Swine Production development.In the past few decades, in daily ration, add antibiotic, to strengthen the premunition of delactational piglets, suppress harmful microorganism to be bred in enteron aisle, reduce the generation of diarrhoeal diseases, effective alleviation ablactation stress with the production performance improving early wea-ning piglet.But, Long-Time Service antibiotic, cause drug Resistance of Pathogenic Microorganism from Surface strengthens and antibiotic is residual in animal product problem extremely people query, therefore, development and application safe, the efficient feed addictive of substitute antibiotics can become the inexorable trend of animal productiong development.In recent years research confirms that the multiple additives such as acidulant, enzyme preparation, medium chain fatty acid, oligosaccharides, Chinese herbal medicine, plant extracts, microorganism formulation all have certain health care growth promoting function.But about mostly these additive effects are the test of single variety, and difference on effect is larger.For this reason, a kind of resisting stress premix promoting piglet efficient and healthful to produce urgently is developed.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of resisting stress premix promoting piglet growth and preparation method thereof, play resisting stress effect from intestinal health, oxidative stress, neurological levels three aspects, improve the anti-stress ability of piglet.
The present invention is achieved by the following technical solutions: a kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate 5-6, phenylacetic acid 2-3, benzoic acid 4-6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, GABA 10-15, composite oligosaccharide 100-150, three solid fermentation green tea slag 465-643.
As the further optimization of such scheme, with parts by weight, composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 120, three solid fermentation green tea slags 617.
As the further optimization of such scheme, with parts by weight, composed of the following components: fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, GABA 15, composite oligosaccharide 130, three solid fermentation green tea slags 548.
As the further optimization of such scheme, described composite oligosaccharide is two kinds and the above compound sugar mixed with arbitrary proportion in mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide.
Promote a preparation method for the resisting stress premix of piglet growth, comprise preparation three solid fermentation green tea slags, prepare three solid fermentation green tea slags and comprise the steps:
(1) fermentate comprise fermentation major ingredient and fermentation auxiliary material, described fermentation major ingredient is green tea slag, by the green tea slag of 60-75% with mix with the fermentation auxiliary material of 30-45% after, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, bacillus subtilis and short and small spore bacillus mix with arbitrary proportion, the mixture that step (1) obtains cultivates 3-5 days at 30-37 DEG C, carry out one time fermentation, in one time fermentation incubation every 2-3h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, described mixed fungus comprises candida utili and aspergillus niger, candida utili and aspergillus niger mix with arbitrary proportion, cultivate 3-5 days at 28-35 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5-7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2-3 days at 30-37 DEG C, carry out three fermentations, in three fermented and cultured processes every 4-6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
As the further optimization of such scheme, described fermentation auxiliary material comprises the dish dregs of rice, cotton dregs and wheat bran, its Chinese food dregs of rice, cotton dregs and, the ratio of wheat bran in fermentate is: dish dregs of rice 10-15%, cotton dregs 10-15% and wheat bran 10-15%.
As the further optimization of such scheme, in one time fermentation, the bacterial concentration of described bacillus subtilis is OD 600=0.5-1, the bacterial concentration OD of described short and small spore bacillus 600=0.5-1, and described bacillus subtilis mixes with volume ratio 1:2 ratio with short and small spore bacillus, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml.
As the further optimization of such scheme, in secondary fermentation, described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus.
Compared with the prior art, the beneficial effect of a kind of resisting stress premix promoting piglet growth of the present invention and preparation method thereof is embodied in:
1, of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth comprises acidulant, solid fermentation green tea slag and microorganism formulation.Add in porkling feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth reduces piglet ight soil humidity, improve delactational piglets survival rate, especially to piglet after wean, good growth performance can also be kept.
2, of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth plays effect from intestinal health, oxidative stress, neurological levels three aspects, there is good effect; Add in pig starter feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth is to the daily gain of piglet, and feed conversion rate there is obvious effect of optimization.
3, of the present inventionly a kind ofly promote that piglet growth is urged in the resisting stress premix of piglet growth, the acidulant contained in premix directly can reduce intestinal pH, changes the enteron aisle acid-base value of piglet, suppresses acid nonfast pathogen to breed.
4, a kind of promotion of the present invention contains three solid fermentation green tea slags in the resisting stress premix of piglet growth, wherein, three times solid fermentation green tea slag is formed by fermentation of bacillus subtilis, bacillus subtilis has obvious inhibiting active material by consuming the oxygen in enteron aisle, the competition mechanism participating in enteron aisle adhesion and generation to pathogenic bacteria, both be the growing environment that the intestinal beneficial such as anaerobic type lactic acid bacteria create anoxic, also effectively suppressed the growth of harmful intestinal tract bacteria simultaneously.
5, a kind of promotion of the present invention contains three solid fermentation green tea slags in the resisting stress premix of piglet growth, the active ingredient mainly Tea Polyphenols of three solid fermentation green tea slags, Tea Polyphenols strengthens piglet anti-stress ability from the oxidativestress damage aspect alleviating hot environment generation.
6, a kind of fumaric acid, citric acid, malic acid, sodium Diacetate, sodium butyrate, phenylacetic acid, green tea slag, mixed bacteria etc. promoting to contain in the resisting stress premix of piglet growth of the present invention all meets forage health standard, the Ministry of Agriculture and national standard, harmful effect can not be caused, safety and environmental protection more to the nutritive value of piglet.
Detailed description of the invention
Elaborate to embodiments of the invention below, the embodiment of the present invention is implemented under premised on the technical program, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30, citric acid 40, malic acid 45, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 2, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 100, three solid fermentation green tea slags 465.
Glutamine in embodiment 1, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 1 is mannan-oligosaccharides and oligomeric maltose mixing oligosaccharide, and wherein mannan-oligosaccharides and oligomeric maltose mix with mass ratio 1:1.
Three solid fermentation green tea slags in embodiment 1 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 70% and 10% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD 600=0.5-1), the bacterial concentration OD of short and small spore bacillus 600=0.5-1), bacillus subtilis and short and small spore bacillus mix with volume ratio 1:2 ratio, the fermentate that step (1) obtains, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100g:1ml, cultivate 3 days at 30 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus, cultivates 3 days at 28 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 5h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 30 DEG C, carry out three fermentations, in three fermented and cultured processes every 4h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 2
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 50, citric acid 60, malic acid 65, sodium Diacetate 5, sodium butyrate 6, phenylacetic acid 3, benzoic acid 6, glutamine 150, yeast selenium 20, astaxanthin 5, GABA 15, composite oligosaccharide 150, three solid fermentation green tea slags 643.
Glutamine in embodiment 2, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 2 is isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:2:2:1.
Three solid fermentation green tea slags in embodiment 2 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 65% and 15% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD 600=0.5-1), the bacterial concentration OD of short and small spore bacillus 600=0.5-1), bacillus subtilis and short and small spore bacillus mix with volume ratio 1:1 ratio, the fermentate that step (1) obtains, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 105g:1.05ml, cultivate 4 days at 33 DEG C, carry out one time fermentation, in one time fermentation incubation every 2.5h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus, cultivates 3.5 days at 30 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 6h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2.5 days at 34 DEG C, carry out three fermentations, in three fermented and cultured processes every 4.5h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 3
A kind of resisting stress premix promoting piglet growth, with parts by weight, composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 120, three solid fermentation green tea slags 617.
Glutamine in embodiment 3, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 3 is isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:3:1.
Three solid fermentation green tea slags in embodiment 3 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 65% and 10% after autoclaving, the cotton dregs of 12% and the wheat bran of 13% being mixed, add fermented bacterium, ferment; Wherein 65% green tea slag, the dish dregs of rice of 10%, the cotton dregs of 12% and 13% wheat bran be mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD 600=0.5-1), the bacterial concentration OD of short and small spore bacillus 600=0.5-1), and bacillus subtilis mixes with volume ratio 1:2 ratio with short and small spore bacillus, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 120g:1.05ml, cultivate 3 days at 37 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus, cultivates 3 days at 35 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 6h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 37 DEG C, carry out three fermentations, in three fermented and cultured processes every 6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Embodiment 4
Promote a resisting stress premix for piglet growth, fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, GABA 15, composite oligosaccharide 130, three solid fermentation green tea slags 548.
Glutamine in embodiment 4, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzoic acid, glutamine, yeast selenium, astaxanthin, GABA are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 4 is isomalto-oligosaccharide and xylo-oligosaccharide mixing oligosaccharide.Wherein isomalto-oligosaccharide and xylo-oligosaccharide take mass ratio as the ratio mixing of 1:1.
Three solid fermentation green tea slags in embodiment 4 are prepared from by following processing technology:
(1), after the fermentation auxiliary material of the dish dregs of rice of the green tea slag of 75% and 10% after autoclaving, the cotton dregs of 10% and the wheat bran of 10% being mixed, add fermented bacterium, ferment; Wherein 65% green tea slag, the dish dregs of rice of 10%, the cotton dregs of 12% and 13% wheat bran be mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, wherein, Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, and the bacterial concentration of bacillus subtilis is OD 600=0.5-1), the bacterial concentration OD of short and small spore bacillus 600=0.5-1), and bacillus subtilis mixes with volume ratio 3:2 ratio with short and small spore bacillus, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 110g:1.03ml, cultivate 4 days at 35 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, takes out the fermentate of step (2), renewed vaccination mixed fungus, wherein, mixed fungus comprises candida utili and aspergillus niger, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:3 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus, cultivates 3 days at 28 DEG C, carries out secondary fermentation, in secondary fermentation incubation every 7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 35 DEG C, carry out three fermentations, in three fermented and cultured processes every 6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
Experiment 1
Employing single factor test contrasts, the a kind of of interpolation the preferred embodiments of the present invention 3 of the present invention is utilized to promote the pig starter feed of the resisting stress premix of piglet growth, conventional pig starter feed and add antibiotic pig starter feed to feed 21-23 age in days weanling pig, in three groups, often organize 126, raise 35 days, respectively at the body weight weighing every piglet by group by head on an empty stomach in morning on the same day of on-test and end, record pig starter feed consumption every day, for calculating the weightening finish of piglet average body, average daily gain, average feed intake, feed-weight ratio and ight soil humidity.Specifically see table 1.
Table 1: the piglet growth performance table of comparisons 1
Experimental group and control group initial weight, without significant difference, after cycling hot stress process, are added the end weight of the test group of resisting stress premix, weightening finish, feed intake and survival rate and are significantly higher than control group.Heat stress obviously reduces meat wild pheasant production performance, and a kind of 1-2 of the present invention age in week, meat wild pheasant anti-heat stress feed can this negative effect of larger alleviation, had good anti-heat stress effect.
As shown in Table 1, use a kind of resisting stress premix promoting piglet growth of the present invention, wean in latter 35 days, add antibiotic and do not add antibiotic pig starter feed and compare with feeding, use a kind of average daily gain and feed efficiency promoting the resisting stress premix of piglet growth and add that antibiotic pig starter feed comparatively significantly improves early wea-ning piglet of the present invention; And the piglet average daily gain adding antibiotic group is the highest, but adds and of the present inventionly a kind ofly promote that the baby pig feedstuff anharmonic ratio of the resisting stress premix group of piglet growth is minimum.Use in pig starter feed and of the present inventionly a kind ofly promote that the resisting stress premix of piglet growth effectively reduces early wea-ning piglet ight soil humidity, reduce baby pig diarrhea, improve the survival rate of early wea-ning piglet.
Experiment 2
Employing single factor test contrasts, the a kind of of interpolation the preferred embodiments of the present invention 4 of the present invention is utilized to promote the pig starter feed of the resisting stress premix of piglet growth, conventional pig starter feed and add antibiotic pig starter feed to feed 21-23 age in days weanling pig, in three groups, often organize 210, raise 35 days, respectively at the body weight weighing every piglet by group by head on an empty stomach in morning on the same day of on-test and end, record pig starter feed consumption every day, for calculating the weightening finish of piglet average body, average daily gain, average feed intake, feed-weight ratio and ight soil humidity.Specifically see table 1.
Table 2: the piglet growth performance table of comparisons 2
As shown in Table 2, use a kind of resisting stress premix promoting piglet growth of the present invention, wean in latter 35 days, average daily gain is compared control group with feed efficiency and is significantly improved, and the survival rate of weanling pig, up to 99%, reduces early wea-ning piglet ight soil humidity simultaneously, reduce baby pig diarrhea, make piglet growth performance obtain good improvement, feed-weight ratio significantly reduces, and saves feeding cost.
From result, feed the body weight that a kind of resisting stress premix promoting piglet growth of the present invention and preparation method thereof effectively improves meat wild pheasant, reduce its death rate and feedstuff-meat ratio, larger economic benefit can be created.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.

Claims (8)

1. one kind promotes the resisting stress premix of piglet growth, it is characterized in that, with parts by weight, composed of the following components: fumaric acid 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate 5-6, phenylacetic acid 2-3, benzoic acid 4-6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, GABA 10-15, composite oligosaccharide 100-150, three solid fermentation green tea slag 465-643.
2. a kind of resisting stress premix promoting piglet growth according to claim 1, it is characterized in that, with parts by weight, composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3, GABA 10, composite oligosaccharide 120, three solid fermentation green tea slags 617.
3. a kind of resisting stress premix promoting piglet growth according to claim 1, it is characterized in that, with parts by weight, composed of the following components: fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, GABA 15, composite oligosaccharide 130, three solid fermentation green tea slags 548.
4. a kind of resisting stress premix promoting piglet growth according to claim 1, it is characterized in that, described composite oligosaccharide is two kinds and the above compound sugar mixed with arbitrary proportion in mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, xylo-oligosaccharide.
5. promote a preparation method for the resisting stress premix of piglet growth, it is characterized in that, comprise preparation three solid fermentation green tea slags, prepare three solid fermentation green tea slags and comprise the steps:
(1) fermentate comprise fermentation major ingredient and fermentation auxiliary material, described fermentation major ingredient is green tea slag, by the green tea slag of 60-75% with mix with the fermentation auxiliary material of 30-45% after, add fermented bacterium, ferment;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis and short and small spore bacillus, bacillus subtilis and short and small spore bacillus mix with arbitrary proportion, the mixture that step (1) obtains cultivates 3-5 days at 30-37 DEG C, carry out one time fermentation, in one time fermentation incubation every 2-3h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, described mixed fungus comprises candida utili and aspergillus niger, candida utili and aspergillus niger mix with arbitrary proportion, cultivate 3-5 days at 28-35 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5-7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2-3 days at 30-37 DEG C, carry out three fermentations, in three fermented and cultured processes every 4-6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation green tea slags.
6. a kind of preparation method promoting the resisting stress premix of piglet growth according to claim 5, it is characterized in that: described fermentation auxiliary material comprises the dish dregs of rice, cotton dregs and wheat bran, its Chinese food dregs of rice, cotton dregs and, the ratio of wheat bran in fermentate is: dish dregs of rice 10-15%, cotton dregs 10-15% and wheat bran 10-15%.
7. a kind of preparation method promoting the resisting stress premix of piglet growth according to claim 5, is characterized in that: in one time fermentation, and the bacterial concentration of described bacillus subtilis is OD 600=0.5-1, the bacterial concentration OD of described short and small spore bacillus 600=0.5-1, and described bacillus subtilis mixes with volume ratio 1:2 ratio with short and small spore bacillus, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml.
8. a kind of preparation method promoting the resisting stress premix of piglet growth according to claim 5, is characterized in that: in secondary fermentation, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10 6-1 × 10 8individual mixed fungus.
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CN105995077A (en) * 2016-05-25 2016-10-12 沈阳马克西玛生物科技有限公司 Fodder additive for piglet, preparation method and fodder thereof
CN107467370A (en) * 2017-10-13 2017-12-15 无锡正大生物股份有限公司 A kind of feed feature phagostimulant and preparation method thereof
CN107712356A (en) * 2017-11-20 2018-02-23 四川檀榕动物营养食品有限公司 A kind of feed addictive and preparation method thereof, feed
CN107996857A (en) * 2017-12-14 2018-05-08 南昌傲农生物科技有限公司 A kind of liquid amino acid vitamine supplement for reducing weaning stress of piglet and preparation method and application
CN108606179A (en) * 2018-05-14 2018-10-02 四川傲农生物科技有限公司 It is a kind of alleviate piglet immunological stress feed addictive and its preparation method and application
CN108887474A (en) * 2018-06-14 2018-11-27 河南省帝方生物制药有限公司 A kind of compound preparation of resisting stress and preparation method thereof
CN109770053A (en) * 2019-01-25 2019-05-21 华中农业大学 A kind of mixed fodder additive for early-weaned piglets
CN109820089A (en) * 2019-03-06 2019-05-31 江苏波杜农牧股份有限公司 A kind of premix and preparation method thereof for alleviating plant-eating animal transport stress
CN111718925A (en) * 2020-08-06 2020-09-29 清远一生自然生物研究院有限公司 Method for preparing tea residue feed by fermenting mixed strains
CN111718925B (en) * 2020-08-06 2023-06-13 清远一生自然生物研究院有限公司 Method for preparing tea dreg feed by fermenting mixed strains

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