CN105076862B - A kind of resisting stress premix and preparation method thereof promoting piglet growth - Google Patents
A kind of resisting stress premix and preparation method thereof promoting piglet growth Download PDFInfo
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Abstract
The invention discloses a kind of resisting stress premixes of promotion piglet growth, in parts by weight, composed of the following components: fumaric acid 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate 5-6, phenylacetic acid 2-3, benzoic acid 4-6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, γ-aminobutyric acid 10-15, composite oligosaccharide 100-150, three times solid fermentation green tea slag 465-643.The present invention has the advantage that a kind of resisting stress premix and preparation method thereof of promotion piglet growth of the invention plays resisting stress effect in terms of intestinal health, oxidative stress, neurological levels three compared with prior art, improve the anti-stress ability of piglet, effectively reduce early wea- ning piglet excrement humidity, reduce baby pig diarrhea, enhance its immunity and disease resistance, improves the survival rate of early wea- ning piglet.
Description
Technical field
The present invention relates to a kind of piglet premix more particularly to it is a kind of promote piglet growth resisting stress premix and
Preparation method.
Background technique
1-3 weeks after weaned piglet, since self immune system is not yet physically well developed, digestion power was weak, and premunition is poor, into
The change dramatically for eating diet and living environment, be easy to make its generation stress, cause grice diarrhoea, feed intake decline, growth retardation,
It is serious or even dead, it is such as mixed or secondary infection, case fatality rate is up to 26% or more.After piglet birth, to ensure its energy
Enough smoothly to tide over wean difficulty, after overcoming wean a series of problems, normal health growth need to create good life for it
Condition, suitable daily ration.
Nutrition stress be cause piglet ablactation stress one of main factor, thus, using the method for nutrition regulation come
Alleviating early weaning piglet stress be an effective way for promoting health, the development of efficient pig production.In the past few decades
In, antibiotic is added in daily ration, to enhance the premunition of delactational piglets, harmful microorganism is inhibited to be proliferated in enteron aisle, reduced
The generation of diarrhoeal diseases, effective alleviation ablactation stress be with the production performances of improvement early wea- ning piglet.However, antibiosis is used for a long time
Element, leads to that drug Resistance of Pathogenic Microorganism from Surface enhances and antibiotic the problem of remaining in animal product is queried by people, therefore,
The safe and efficient feed addictive that substitute antibiotics are capable of in development and application has become the inexorable trend of animal productiong development.
In recent years research confirms that acidulant, enzyme preparation, medium chain fatty acid, oligosaccharides, Chinese herbal medicine, plant extracts, microorganism formulation etc. are more
Kind additive all has certain health care growth promoting function.But it is mostly the test of single variety in relation to these additive effects, and
Difference on effect is larger.Thus, it would be highly desirable to develop a kind of resisting stress premix of promotion piglet efficient and healthful production.
Summary of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of resisting stress premixs of promotion piglet growth
Material and preparation method thereof plays resisting stress effect in terms of intestinal health, oxidative stress, neurological levels three, improves piglet
Anti-stress ability.
The present invention is achieved by the following technical solutions: a kind of resisting stress premix promoting piglet growth, with weight
Part meter, it is composed of the following components: fumaric acid 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate
5-6, phenylacetic acid 2-3, benzoic acid 4-6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, γ-aminobutyric acid 10-
15, composite oligosaccharide 100-150, three times solid fermentation green tea slag 465-643.
It is in parts by weight, composed of the following components as advanced optimizing for above scheme: fumaric acid 30, citric acid
45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, astaxanthin 3,
γ-aminobutyric acid 10, composite oligosaccharide 120, three times solid fermentation green tea slag 617.
It is in parts by weight, composed of the following components as advanced optimizing for above scheme: fumaric acid 40, citric acid
50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5,
γ-aminobutyric acid 15, composite oligosaccharide 130, three times solid fermentation green tea slag 548.
As advanced optimizing for above scheme, the composite oligosaccharide is mannan-oligosaccharides, oligomeric maltose, different malt
Oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, two kinds in xylo-oligosaccharide and its more than the oligosaccharide that is mixed with arbitrary proportion.
A kind of preparation method for the resisting stress premix promoting piglet growth, including solid fermentation green tea slag three times is prepared,
Solid fermentation green tea slag includes the following steps: three times for preparation
(1) fermentation material includes fermentation major ingredient and fermentation auxiliary material, and the fermentation major ingredient is green tea slag, by the green tea of 60-75%
After slag is mixed with the fermentation auxiliary material of 30-45%, fermenting microbe is added, ferments；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis
And bacillus pumilus, bacillus subtilis and bacillus pumilus are mixed with arbitrary proportion, the mixture that step (1) obtains exists
30-37 DEG C culture 3-5 days, carry out one time fermentation, it is primary every 2-3h overturning in one time fermentation incubation；
(3) secondary fermentation: after one time fermentation, the fermentation material of step (2) is taken out, renewed vaccination mixed fungus is described
Mixed fungus includes candida utili and aspergillus niger, and candida utili and aspergillus niger are mixed with arbitrary proportion, at 28-35 DEG C
Culture 3-5 days carries out secondary fermentation, primary every 5-7h overturning in secondary fermentation incubation；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 5-6, again
Be inoculated with German-style Lactobacillus lactate subspecies, 30-37 DEG C culture 2-3 days, fermented three times, during fermented and cultured three times often
It is primary every 4-6h overturning；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation three times
Green tea slag.
As advanced optimizing for above scheme, the fermentation auxiliary material includes rapeseed meal, cotton dregs and wheat bran, wherein rapeseed meal, cotton
The ratio of the dregs of rice and wheat bran in fermentation material are as follows: rapeseed meal 10-15%, cotton dregs 10-15% and wheat bran 10-15%.
As advanced optimizing for above scheme, in one time fermentation, the bacterial concentration of the bacillus subtilis is OD600
=0.5-1, the bacterial concentration OD of the bacillus pumilus600=0.5-1, and the bacillus subtilis and short and small gemma bar
Bacterium is with the mixing of volume ratio 1:2 ratio, and fermentation material and Mixed Microbes are with the ratio mixed fermentation of 100-120g:1-1.05ml.
As advanced optimizing for above scheme, in secondary fermentation, the candida utili and aspergillus niger are with volume ratio
The mixing of 1:2 ratio, every 100g fermentation material inoculation 1 × 106-1×108A mixed fungus.
Compared with the prior art, resisting stress premix of a kind of promotion piglet of the invention growth and preparation method thereof has
Beneficial effect is embodied in:
1, include in a kind of resisting stress premix of promotion piglet growth of the invention acidulant, solid fermentation green tea slag and
Microorganism formulation.The resisting stress premix that a kind of promotion piglet growth of the invention is added in porkling feed reduces piglet excrement
Humidity improves delactational piglets survival rate, especially to piglet after wean, moreover it is possible to keep preferable growth performance.
2, a kind of resisting stress premix of promotion piglet growth of the invention is from intestinal health, oxidative stress, neurological levels
Three aspects play effect, have preferable application effect；A kind of promotion piglet growth of the invention is added in pig starter feed
Resisting stress premix has apparent effect of optimization to the daily gain and feed conversion rate of piglet.
3, a kind of resisting stress premix of promotion piglet of the invention growth promotees piglet growth, the acidification contained in premix
Agent can directly reduce intestinal pH, change the enteron aisle pH value of piglet, and acid nonfast pathogen is inhibited to be proliferated.
4, contain solid fermentation green tea slag three times in a kind of resisting stress premix of promotion piglet of the invention growth,
In, solid fermentation green tea slag is formed by fermentation of bacillus subtilis three times, and bacillus subtilis passes through the oxygen in consumption enteron aisle
Gas, the competition mechanism for participating in enteron aisle adherency and generation have the active material of obvious inhibiting effect to pathogenic bacteria, have both been anaerobic type cream
The intestinal beneficials such as sour bacterium create the growing environment of anoxic, while also effectively inhibiting the growth of harmful intestinal tract bacteria.
5, containing solid fermentation green tea slag three times in a kind of resisting stress premix of promotion piglet of the invention growth, three times
The effective component of solid fermentation green tea slag is mainly tea polyphenols, and tea polyphenols are from the oxidativestress damage side alleviating hot environment and generating
Face enhances piglet anti-stress ability.
6, fumaric acid, citric acid, the apple contained in a kind of resisting stress premix of promotion piglet of the invention growth
Acid, sodium Diacetate, sodium butyrate, phenylacetic acid, green tea slag, mixed bacteria etc. meet forage health standard, the Ministry of Agriculture and country's mark
Standard will not cause adverse effect, more safety and environmental protection to the nutritive value of piglet.
Specific embodiment
It elaborates below to the embodiment of the present invention, the embodiment of the present invention carries out under premised on the technical program
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of resisting stress premix promoting piglet growth, in parts by weight, composed of the following components: fumaric acid 30,
Citric acid 40, malic acid 45, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 2, benzoic acid 4, glutamine 100, yeast selenium 10, shrimp are green
Plain 3, γ-aminobutyric acid 10, composite oligosaccharide 100, three times solid fermentation green tea slag 465.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 1
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 1 is mannan-oligosaccharides and oligomeric maltose mixing oligosaccharide, and wherein sweet dew is oligomeric
Sugar and oligomeric maltose are with mass ratio 1:1 mixing.
The green tea of solid fermentation three times slag in embodiment 1 is prepared by following processing technologys:
(1) by 70% green tea slag and 10% rapeseed meal after high pressure sterilization, 10% cotton dregs and 10% wheat bran
It ferments after auxiliary material mixing, fermenting microbe is added, ferments；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, wherein Mixed Microbes include bacillus subtilis
And bacillus pumilus, the bacterial concentration of bacillus subtilis are OD600=0.5-1), the bacterial concentration of bacillus pumilus
OD600=0.5-1), bacillus subtilis and bacillus pumilus are with the mixing of volume ratio 1:2 ratio, the fermentation that step (1) obtains
Object, fermentation material and Mixed Microbes are cultivated 3 days with the ratio mixed fermentation of 100g:1ml at 30 DEG C, are carried out one time fermentation, are once being sent out
It is primary every 2h overturning in ferment incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus secondary fermentation: are taken out, wherein
Mixed fungus includes candida utili and aspergillus niger, and the candida utili and aspergillus niger are mixed with volume ratio 1:2 ratio,
Every 100g fermentation material inoculation 1 × 106-1×108A mixed fungus is cultivated 3 days at 28 DEG C, secondary fermentation is carried out, in secondary fermentation
It is primary every 5h overturning in incubation；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 5-6, again
German-style Lactobacillus lactate subspecies are inoculated with, cultivates 2 days, is fermented three times, every 4h during fermented and cultured three times at 30 DEG C
Overturning is primary；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation three times
Green tea slag.
Embodiment 2
A kind of resisting stress premix promoting piglet growth, in parts by weight, composed of the following components: fumaric acid 50,
Citric acid 60, malic acid 65, sodium Diacetate 5, sodium butyrate 6, phenylacetic acid 3, benzoic acid 6, glutamine 150, yeast selenium 20, shrimp are green
Plain 5, γ-aminobutyric acid 15, composite oligosaccharide 150, three times solid fermentation green tea slag 643.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 2
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 2 is that isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide are mixed
Close oligosaccharide.Wherein isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides and xylo-oligosaccharide are 1:2:2:1's with mass ratio
Ratio mixing.
The green tea of solid fermentation three times slag in embodiment 2 is prepared by following processing technologys:
(1) by 65% green tea slag and 15% rapeseed meal after high pressure sterilization, 10% cotton dregs and 10% wheat bran
It ferments after auxiliary material mixing, fermenting microbe is added, ferments；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, wherein Mixed Microbes include bacillus subtilis
And bacillus pumilus, the bacterial concentration of bacillus subtilis are OD600=0.5-1), the bacterial concentration of bacillus pumilus
OD600=0.5-1), bacillus subtilis and bacillus pumilus are with the mixing of volume ratio 1:1 ratio, the fermentation that step (1) obtains
Object, fermentation material and Mixed Microbes are cultivated 4 days with the ratio mixed fermentation of 105g:1.05ml at 33 DEG C, one time fermentation are carried out, one
It is primary every 2.5h overturning during secondary fermented and cultured；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus secondary fermentation: are taken out, wherein
Mixed fungus includes candida utili and aspergillus niger, and candida utili and aspergillus niger are with the mixing of volume ratio 1:1 ratio, often
100g fermentation material inoculation 1 × 106-1×108A mixed fungus is cultivated 3.5 days at 30 DEG C, secondary fermentation is carried out, in secondary fermentation
It is primary every 6h overturning in incubation；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 5-6, again
Be inoculated with German-style Lactobacillus lactate subspecies, 34 DEG C cultivate 2.5 days, fermented three times, during fermented and cultured three times every
4.5h overturning is primary；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation three times
Green tea slag.
Embodiment 3
A kind of resisting stress premix promoting piglet growth, in parts by weight, composed of the following components: fumaric acid 30,
Citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzoic acid 4, glutamine 100, yeast selenium 10, shrimp are green
Plain 3, γ-aminobutyric acid 10, composite oligosaccharide 120, three times solid fermentation green tea slag 617.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 3
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 3 is isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide mixing oligosaccharide.Its
Middle isomalto-oligosaccharide, fructose oligosaccharides and xylo-oligosaccharide are mixed with mass ratio for the ratio of 1:3:1.
The green tea of solid fermentation three times slag in embodiment 3 is prepared by following processing technologys:
(1) by 65% green tea slag and 10% rapeseed meal after high pressure sterilization, 12% cotton dregs and 13% wheat bran
It ferments after auxiliary material mixing, fermenting microbe is added, ferments；Wherein 65% green tea slag, 10% rapeseed meal, 12% cotton dregs and
13% wheat bran is mass percent.
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, wherein Mixed Microbes include bacillus subtilis
And bacillus pumilus, the bacterial concentration of bacillus subtilis are OD600=0.5-1), the bacterial concentration of bacillus pumilus
OD600=0.5-1), and bacillus subtilis and bacillus pumilus are mixed with volume ratio 1:2 ratio, the hair that step (1) obtains
Ferment object and Mixed Microbes are cultivated 3 days at 37 DEG C with the ratio mixed fermentation of 120g:1.05ml, one time fermentation are carried out, in one time fermentation
It is primary every 2h overturning in incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus secondary fermentation: are taken out, wherein
Mixed fungus includes candida utili and aspergillus niger, and candida utili and aspergillus niger are with the mixing of volume ratio 1:2 ratio, often
100g fermentation material inoculation 1 × 106-1×108A mixed fungus is cultivated 3 days at 35 DEG C, carries out secondary fermentation, is trained in secondary fermentation
It is primary every 6h overturning during supporting；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 6, is connect again
Kind German-style Lactobacillus lactate subspecies, cultivate 2 days at 37 DEG C, are fermented, turned over during fermented and cultured three times every 6h three times
Turn primary；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation three times
Green tea slag.
Embodiment 4
A kind of resisting stress premix promoting piglet growth, fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate
4, sodium butyrate 5, phenylacetic acid 3, benzoic acid 5, glutamine 120, yeast selenium 15, astaxanthin 5, γ-aminobutyric acid 15, compound oligomeric
Sugar 130, three times solid fermentation green tea slag 548.
Glutamine, fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid, benzene in embodiment 4
Formic acid, glutamine, yeast selenium, astaxanthin, γ-aminobutyric acid are the convenience goods bought on the market, and purity is feed grade.
Composite oligosaccharide in embodiment 4 is isomalto-oligosaccharide and xylo-oligosaccharide mixing oligosaccharide.Wherein different malt is low
Glycan and xylo-oligosaccharide are mixed with mass ratio for the ratio of 1:1.
The green tea of solid fermentation three times slag in embodiment 4 is prepared by following processing technologys:
(1) by 75% green tea slag and 10% rapeseed meal after high pressure sterilization, 10% cotton dregs and 10% wheat bran
It ferments after auxiliary material mixing, fermenting microbe is added, ferments；Wherein 65% green tea slag, 10% rapeseed meal, 12% cotton dregs and
13% wheat bran is mass percent.
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, wherein Mixed Microbes include bacillus subtilis
And bacillus pumilus, the bacterial concentration of bacillus subtilis are OD600=0.5-1), the bacterial concentration of bacillus pumilus
OD600=0.5-1), and bacillus subtilis and bacillus pumilus are mixed with volume ratio 3:2 ratio, the hair that step (1) obtains
Ferment object and Mixed Microbes are cultivated 4 days at 35 DEG C with the ratio mixed fermentation of 110g:1.03ml, one time fermentation are carried out, in one time fermentation
It is primary every 2h overturning in incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus secondary fermentation: are taken out, wherein
Mixed fungus includes candida utili and aspergillus niger, and the candida utili and aspergillus niger are mixed with volume ratio 1:3 ratio,
Every 100g fermentation material inoculation 1 × 106-1×108A mixed fungus is cultivated 3 days at 28 DEG C, secondary fermentation is carried out, in secondary fermentation
It is primary every 7h overturning in incubation；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 6, is connect again
Kind German-style Lactobacillus lactate subspecies, cultivate 2 days at 35 DEG C, are fermented, turned over during fermented and cultured three times every 6h three times
Turn primary；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation three times
Green tea slag.
Experiment 1
It is compared using single factor test, is grown using a kind of promotion piglet of addition the preferred embodiment of the present invention 3 of the invention
The pig starter feed of resisting stress premix, conventional pig starter feed and add the pig starter feed of antibiotic to feed 21-23 age in days disconnected
Milk piglet in three groups, every group 126, is raised 35 days, claims on an empty stomach by group by head respectively at on-test and the morning on the same day of end
Measure the weight of every piglet, daily record pig starter feed consumption, for calculate piglet be averaged body weight gains, average daily gain, put down
Equal feed intake, feed-weight ratio and excrement humidity.Referring specifically to table 1.
Table 1: the piglet growth performance table of comparisons 1
As shown in Table 1, the resisting stress premix grown using a kind of promotion piglet of the invention, after wean in 35 days, with
Feeding addition antibiotic is compared with the pig starter feed for not adding antibiotic, the resisting stress grown using a kind of promotion piglet of the invention
The pig starter feed of premix and addition antibiotic relatively significantly improves the average daily gain and food conversion of early wea- ning piglet
Efficiency；And the piglet average daily gain highest of antibiotic group is added, but add a kind of promotion piglet growth of the invention
The piglet feed-weight ratio of resisting stress premix group is minimum.A kind of anti-the answering of promotion piglet growth of the invention is used in pig starter feed
Sharp premix effectively reduces early wea- ning piglet excrement humidity, reduces baby pig diarrhea, improves surviving for early wea- ning piglet
Rate.
Experiment 2
It is compared using single factor test, is grown using a kind of promotion piglet of addition the preferred embodiment of the present invention 4 of the invention
The pig starter feed of resisting stress premix, conventional pig starter feed and add the pig starter feed of antibiotic to feed 21-23 age in days disconnected
Milk piglet in three groups, every group 210, is raised 35 days, claims on an empty stomach by group by head respectively at on-test and the morning on the same day of end
Measure the weight of every piglet, daily record pig starter feed consumption, for calculate piglet be averaged body weight gains, average daily gain, put down
Equal feed intake, feed-weight ratio and excrement humidity.Referring specifically to table 1.
Table 2: the piglet growth performance table of comparisons 2
As shown in Table 2, the resisting stress premix grown using a kind of promotion piglet of the invention after wean in 35 days, is put down
Equal daily gain and feed efficiency significantly improve compared to the control group, and the high survival rate of weanling pig reduces simultaneously up to 99%
Early wea- ning piglet excrement humidity reduces baby pig diarrhea, so that piglet growth performance is obtained good improvement, feed-weight ratio significantly drops
It is low, save feeding cost.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (7)
1. a kind of resisting stress premix for promoting piglet growth, which is characterized in that in parts by weight, composed of the following components: to prolong
Fumarate 30-50, citric acid 40-60, malic acid 45-65, sodium Diacetate 3-5, sodium butyrate 5-6, phenylacetic acid 2-3, benzoic acid 4-
6, glutamine 100-150, yeast selenium 10-20, astaxanthin 3-5, γ-aminobutyric acid 10-15, composite oligosaccharide 100-150, three
Secondary solid fermentation green tea slag 465-643；Wherein, the green tea slag of solid fermentation three times, is made by the steps:
(1) fermentation material includes fermentation major ingredient and fermentation auxiliary material, and the fermentation major ingredient is green tea slag, by the green tea slag of 60-75% and
After the fermentation auxiliary material mixing of 30-45%, fermenting microbe is added, ferments；
(2) one time fermentation: the fermenting microbe that step (1) uses is Mixed Microbes, and the Mixed Microbes include bacillus subtilis and short
Bacillus pumilus, bacillus subtilis and bacillus pumilus are mixed with arbitrary proportion, and the mixture that step (1) obtains is in 30-
37 DEG C are cultivated 3-5 days, and one time fermentation is carried out, primary every 2-3h overturning in one time fermentation incubation；
(3) after one time fermentation, the fermentation material of step (2), renewed vaccination mixed fungus, the mixing secondary fermentation: are taken out
Fungi includes candida utili and aspergillus niger, and candida utili and aspergillus niger are mixed with arbitrary proportion, is cultivated at 28-35 DEG C
3-5 days, secondary fermentation is carried out, it is primary every 5-7h overturning in secondary fermentation incubation；
(4) it ferments three times: after secondary fermentation, taking out the fermentation material of step (3), adjusting fermentation material pH is 5-6, renewed vaccination
German-style Lactobacillus lactate subspecies, 30-37 DEG C culture 2-3 days, fermented three times, every 4- during fermented and cultured three times
6h overturning is primary；
(5) it dries: after fermentation through step (4), the fermentation material that baking step (4) obtains, as solid fermentation green tea three times
Slag.
2. a kind of resisting stress premix for promoting piglet growth according to claim 1, which is characterized in that with parts by weight
Meter, it is composed of the following components: fumaric acid 30, citric acid 45, malic acid 50, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 3, benzene first
Sour 4, glutamine 100, yeast selenium 10, astaxanthin 3, γ-aminobutyric acid 10, composite oligosaccharide 120, three times solid fermentation green tea
Slag 617.
3. a kind of resisting stress premix for promoting piglet growth according to claim 1, which is characterized in that with parts by weight
Meter, it is composed of the following components: fumaric acid 40, citric acid 50, malic acid 60, sodium Diacetate 4, sodium butyrate 5, phenylacetic acid 3, benzene first
Sour 5, glutamine 120, yeast selenium 15, astaxanthin 5, γ-aminobutyric acid 15, composite oligosaccharide 130, three times solid fermentation green tea
Slag 548.
4. a kind of resisting stress premix for promoting piglet growth according to claim 1, which is characterized in that described compound low
Glycan is mannan-oligosaccharides, oligomeric maltose, isomalto-oligosaccharide, fructose oligosaccharides, soyabean oligosaccharides, two in xylo-oligosaccharide
Kind and its oligosaccharide mixed above with arbitrary proportion.
5. a kind of resisting stress premix for promoting piglet growth according to claim 1 to 4, it is characterised in that: described
Fermentation auxiliary material includes rapeseed meal, cotton dregs and wheat bran, wherein the ratio of rapeseed meal, cotton dregs and wheat bran in fermentation material are as follows: rapeseed meal 10-
15%, cotton dregs 10-15% and wheat bran 10-15%.
6. a kind of resisting stress premix for promoting piglet growth according to claim 1 to 4, it is characterised in that: primary
In fermentation, the bacterial concentration of the bacillus subtilis is OD600=0.5-1, the bacterial concentration OD of the bacillus pumilus600
=0.5-1, and the bacillus subtilis and bacillus pumilus with the mixing of volume ratio 1:2 ratio, fermentation material and Mixed Microbes with
100-120g:1-1.05ml ratio mixed fermentation.
7. a kind of resisting stress premix for promoting piglet growth according to claim 1 to 4, it is characterised in that: secondary
In fermentation, the candida utili and aspergillus niger are with the mixing of volume ratio 1:2 ratio, every 100g fermentation material inoculation 1 × 106-1×
108A mixed fungus.
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| CN105995077A (en) * | 2016-05-25 | 2016-10-12 | 沈阳马克西玛生物科技有限公司 | Fodder additive for piglet, preparation method and fodder thereof |
| CN107467370A (en) * | 2017-10-13 | 2017-12-15 | 无锡正大生物股份有限公司 | A kind of feed feature phagostimulant and preparation method thereof |
| CN107712356A (en) * | 2017-11-20 | 2018-02-23 | 四川檀榕动物营养食品有限公司 | A kind of feed addictive and preparation method thereof, feed |
| CN107996857A (en) * | 2017-12-14 | 2018-05-08 | 南昌傲农生物科技有限公司 | A kind of liquid amino acid vitamine supplement for reducing weaning stress of piglet and preparation method and application |
| CN108606179A (en) * | 2018-05-14 | 2018-10-02 | 四川傲农生物科技有限公司 | It is a kind of alleviate piglet immunological stress feed addictive and its preparation method and application |
| CN108887474A (en) * | 2018-06-14 | 2018-11-27 | 河南省帝方生物制药有限公司 | A kind of compound preparation of resisting stress and preparation method thereof |
| CN109770053A (en) * | 2019-01-25 | 2019-05-21 | 华中农业大学 | A kind of mixed fodder additive for early-weaned piglets |
| CN109820089A (en) * | 2019-03-06 | 2019-05-31 | 江苏波杜农牧股份有限公司 | A kind of premix and preparation method thereof for alleviating plant-eating animal transport stress |
| CN111718925B (en) * | 2020-08-06 | 2023-06-13 | 清远一生自然生物研究院有限公司 | Method for preparing tea dreg feed by fermenting mixed strains |
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| CN101455276B (en) * | 2009-01-09 | 2011-06-29 | 禹善秋 | Feedstuff additive capable of resisting bad stress |
| CN101999523B (en) * | 2010-09-20 | 2012-07-11 | 河南普爱饲料股份有限公司 | Feed additive for resisting heat stress and promoting growth and preparation method thereof |
| CN104839447B (en) * | 2014-02-17 | 2018-05-15 | 天津恒基利得生物科技发展有限公司 | A kind of pregnant sow anti-heat stress feed additive |
| CN104839514A (en) * | 2015-06-16 | 2015-08-19 | 漳州傲农牧业科技有限公司 | Feed additive capable of improving irritability resistance of weaning piglets, as well as preparation and application of feed additive |
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Inventor after: Wen Aiyou Inventor after: Hua Jinling Inventor after: Wang Like Inventor after: Zhu Yuanzhao Inventor after: Dai Sifa Inventor after: Hu Hong Inventor after: Bai Xi Inventor after: Gao Rusong Inventor after: Wang Shujuan Inventor after: Xu Jingen Inventor after: Ren Qingchang Inventor after: Zhao Lei Inventor before: Hu Hong Inventor before: Hua Jinling Inventor before: Wang Like Inventor before: Zhu Yuanzhao Inventor before: Dai Sifa Inventor before: Bai Xi Inventor before: Wen Aiyou Inventor before: Gao Rusong Inventor before: Wang Shujuan Inventor before: Xu Jingen Inventor before: Ren Qingchang Inventor before: Zhao Lei |
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Effective date of registration: 20211220 Address after: 261000 No. 8878, Weichang Road, nanlebu village, lebushan ecological and Economic Development Zone, Weicheng District, Weifang City, Shandong Province Patentee after: Shandong Dayi Biotechnology Group Co.,Ltd. Address before: 233000 eastern suburb of bengshan District, Bengbu City, Anhui Province Patentee before: ANHUI SCIENCE AND TECHNOLOGY University |