CN105076704A - Heat stress resisting premix for laying hen and preparation method of heat stress resisting premix - Google Patents
Heat stress resisting premix for laying hen and preparation method of heat stress resisting premix Download PDFInfo
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Abstract
The invention discloses heat stress resisting premix for laying hen, which is prepared from the following components in parts by weight: 90-130 parts of glutamine, 5-10 parts of citric acid, 1-2 parts of fumaric acid, 2-3 parts of sodium acetate, 2-3 parts of sodium diacetate, 4-5 parts of sodium butyrate, 1-2 parts of phenylacetic acid, 0.1-0.15 part of zinc bacitracin, 6-8 parts of benzoic acid, 15-30 parts of selenium yeast, 2-4 parts of astaxanthin, 8-12 parts of gamma-aminobutyric acid, 100-150 parts of oligosaccharide and 650-800 parts of three-times solid fermentation black tea leaves. Compared with the prior art, the invention has the advantages that the heat stress resisting premix for laying hen and the preparation method of the heat stress resisting premix can effectively lower the pH of the digestive tract of the laying hen, inhibit the growth of harmful microbes, promote the breeding of beneficial microorganisms, optimize the intestinal tract fauna, improve the nutrient substance digestibility, enhance the heat stress resisting capacity of the laying hen, enhance the immunity and the disease resistance of the laying hen, and improve the laying rate.
Description
Technical field
The present invention relates to a kind of egg feedstuff, in particular a kind of laying hen premix material of anti heat stress and preparation method thereof.
Background technology
The chicken of laying hen for laying eggs.Different with broiler chicken, the raising of laying hen focuses on and improves egg production and Egg Quality.Along with the develop rapidly of poultry cultivation technology, the large-scale cultivation of laying hen is very general, but in the process of large-scale cultivation; because cultivation density is larger; cause hen house to dispel the heat difficult, temperature drift in hen house, especially in the summer that temperature is too high; in addition laying hen feather is abundant; metabolism is vigorous, and chicken itself does not have sweat gland perspire to lower the temperature, but by increasing the number of times opening one's mouth to breathe; discharge moisture in lung, help heat radiation.Too high temperature can make laying hen produce heat stress, occur that appetite and feed intake decline, nutrition supply is not enough, and (laying rate declines, and egg size alleviates to cause performance in layers to decline, eggshell strength dies down, yolk color is thin out), serious appearance immunity of layer chicken declines, even dead, cause great economic loss, under research alleviation summer high temperature environment, the counter-measure of heat stress of layer is significant.
The suitable growth temperature of laying hen is 15-25 DEG C, when room temperature is more than 25 DEG C, will heat stress be produced, summer Shanghai, Jiangsu, the ground temperature such as Anhui are greater than 29 DEG C usually, for guaranteeing that laying hen in the event of high temperatures, still having comparatively high productivity energy, needing the Diet Formula of suitably adjustment laying hen, supplementing of reinforced nutrition matter, alleviates the harm that heat stress produces laying hen.Therefore according to feeding standard, urgently develop a kind of successful, act on stable a kind of laying hen premix material of anti heat stress.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of laying hen premix material of anti heat stress and preparation method thereof, play resisting stress effect from intestinal health, oxidative stress, neurological levels three aspects, improve the anti-heat stress ability of laying hen.
The present invention is achieved by the following technical solutions: a kind of laying hen premix material of anti heat stress, it is characterized in that, with parts by weight, composed of the following components: glutamine 90-130, citric acid 5-10, fumaric acid 1-2, sodium acetate 2-3, sodium Diacetate 2-3, sodium butyrate 4-5, phenylacetic acid 1-2, Bacitracin Zinc 0.1-0.15, benzoic acid 6-8, yeast selenium 15-30, astaxanthin 2-4, GABA 8-12, oligosaccharides 100-150, three solid fermentation black tea slag 650-800.
As the further optimization of such scheme, with parts by weight, composed of the following components: glutamine 130, citric acid 5, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate g, phenylacetic acid g, Bacitracin Zinc 0.13, benzoic acid 8, yeast selenium 30, astaxanthin 3, GABA 10, oligosaccharides 140, three solid fermentation black tea slags 662.87.
As the further optimization of such scheme, with parts by weight, composed of the following components: glutamine 125, citric acid 6, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate 5, phenylacetic acid 2, Bacitracin Zinc 0.15, benzoic acid 7, yeast selenium 20, astaxanthin 4, GABA 11, oligosaccharides 150, three solid fermentation black tea slags 664.85.
A preparation method for laying hen premix material of anti heat stress, comprises preparation three solid fermentation black tea slags, prepares three solid fermentation black tea slags and comprises the steps:
(1) fermentate comprises fermentation major ingredient and fermentation auxiliary material, and described fermentation major ingredient is black tea slag, after being mixed by the black tea slag of 65-80%, adds fermented bacterium, ferments with the fermentation auxiliary material of 20-35%;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis, short and small spore bacillus and postpones Bacillus, bacillus subtilis, short and small spore bacillus and delay Bacillus mix with arbitrary proportion, the mixture that step (1) obtains cultivates 3-4 days at 30-37 DEG C, carry out one time fermentation, in one time fermentation incubation every 1-2h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, described mixed fungus comprises candida utili and aspergillus niger, candida utili and aspergillus niger mix with arbitrary proportion, cultivate 3-5 days at 28-32 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5-7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2-3 days at 30-37 DEG C, carry out three fermentations, in three fermented and cultured processes every 5-6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation black tea slags.
As the further optimization of such scheme, described fermentation auxiliary material comprises the dish dregs of rice, cotton dregs and wheat bran, its Chinese food dregs of rice, cotton dregs and, the mass percent of wheat bran in fermentate is: dish dregs of rice 7-15%, cotton dregs 7-15% and wheat bran 6-15%.
As the further optimization of such scheme, in one time fermentation, the bacterial concentration of described bacillus subtilis is OD
600=0.5-1, the bacterial concentration OD of described short and small spore bacillus
600=0.5-1, the bacterial concentration of described delay bacillus is OD
600=0.5-1, and described bacillus subtilis, short and small spore bacillus with delay bacillus mix with volume ratio 1:2:1 ratio, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml.
As the further optimization of such scheme, in secondary fermentation, described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus.
Compared with the prior art, the beneficial effect of a kind of laying hen premix material of anti heat stress of the present invention and preparation method thereof is embodied in:
1, in a kind of laying hen premix material of anti heat stress of the present invention, comprise fumaric acid, citric acid, sodium Diacetate, sodium acetate, sodium butyrate, phenylacetic acid and benzoic acid, the pH in digestive tract of effective reduction laying hen, suppress harmful microbe growth, promote the self immune system of laying hen, suppress the growth of pathogenic microorganism, improve nutritive digestibility, strengthen the anti-heat stress ability of laying hen.
2, a kind of laying hen premix material of anti heat stress of the present invention, under heat stress condition, can meet the demand of laying hen to nutriment, improves laying hen feed intake and immunocompetence, makes laying hen under the condition of Heat-temperature stress, keep good production performance.
3, a kind of laying hen premix material of anti heat stress of the present invention, the feedstuff, glutamine, sodium Diacetate, black tea slag, mixed bacteria etc. that use all meet forage health standard, the Ministry of Agriculture and national standard, harmful effect can not be caused, safety and environmental protection more to the nutritive value of laying hen.
4, a kind of preparation method of laying hen premix material of anti heat stress, mainly for the preparation of three solid fermentation black tea slags, three fermentations are carried out to black tea slag, by bacterial fermentation, fungi fermentation, black tea slag is fully fermented, the conversion ratio of Tea Polyphenols is high, and Tea Polyphenols is from the anti-heat stress ability alleviating the oxidativestress damage aspect enhancing laying hen that hot environment produces.
Detailed description of the invention
Elaborate to embodiments of the invention below, the embodiment of the present invention is implemented under premised on the technical program, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
A kind of laying hen premix material of anti heat stress, with parts by weight, composed of the following components: glutamine 90, citric acid 5, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate 4, phenylacetic acid 1, Bacitracin Zinc 0.1, benzoic acid 6, yeast selenium 15, astaxanthin 2, GABA 8, oligosaccharides 100, three solid fermentation black tea slags 650.
Wherein, glutamine, citric acid, fumaric acid, sodium acetate, sodium Diacetate, sodium butyrate, phenylacetic acid, Bacitracin Zinc, benzoic acid, yeast selenium, astaxanthin, GABA, oligosaccharide, be the convenience goods can bought on the market, purity is feed grade.
Three solid fermentation black tea slags in embodiment 1 are prepared from by following processing technology:
(1) with the black tea slag of 65% and the dish dregs of rice of 15% after autoclaving, the cotton dregs of 10% and 10% wheat bran mixing after, add fermented bacterium, ferment in 1000ml triangular flask; Wherein, black tea slag, the 15% dish dregs of rice, 10% cotton dregs and 10% wheat bran of 65% are mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis, short and small spore bacillus and postpones bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1, the bacterial concentration OD of short and small spore bacillus
600=0.5-1, the bacterial concentration postponing bacillus is OD
600=0.5-1, and bacillus subtilis, short and small spore bacillus with delay bacillus mix with volume ratio 1:2:1 ratio, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml, cultivate 4 days at 35 DEG C, carry out one time fermentation, in one time fermentation incubation every 2h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, mixed fungus comprises aspergillus oryzae and aspergillus niger, and aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, every 100g fermentate inoculation 1 × 10
6-1 × 10
8individual mixed fungus.Cultivate 5 days at 31 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 6h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 35 DEG C, carry out three fermentations, in three fermented and cultured processes every 6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation black tea slags.
Embodiment 2
A kind of laying hen premix material of anti heat stress, with parts by weight, composed of the following components: glutamine 130, citric acid 10, fumaric acid 2, sodium acetate 3, sodium Diacetate 3, sodium butyrate 5, phenylacetic acid 2, Bacitracin Zinc 0.15, benzoic acid 8, yeast selenium 30, astaxanthin 4, GABA 12, oligosaccharides 150, three solid fermentation black tea slags 800.
Raw material sources in embodiment 2 are with embodiment 1, and preparation method's preparation that three of the present embodiment solid fermentation black tea slags are provided by embodiment 1.
Embodiment 3
A kind of laying hen premix material of anti heat stress, with parts by weight, composed of the following components: glutamine 130, citric acid 5, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate g, phenylacetic acid g, Bacitracin Zinc 0.13, benzoic acid 8, yeast selenium 30, astaxanthin 3, GABA 10, oligosaccharides 140, three solid fermentation black tea slags 662.87.
Raw material sources in embodiment 3 are with embodiment 1, and preparation method's preparation that three of the present embodiment solid fermentation black tea slags are provided by embodiment 1.
Embodiment 4
A kind of laying hen premix material of anti heat stress, with parts by weight, composed of the following components: glutamine 125, citric acid 6, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate 5, phenylacetic acid 2, Bacitracin Zinc 0.15, benzoic acid 7, yeast selenium 20, astaxanthin 4, GABA 11, oligosaccharides 150, three solid fermentation black tea slags 664.85.
Wherein, glutamine, citric acid, fumaric acid, sodium acetate, sodium Diacetate, sodium butyrate, phenylacetic acid, Bacitracin Zinc, benzoic acid, yeast selenium, astaxanthin, GABA, oligosaccharide, be the convenience goods can bought on the market, purity is feed grade.
Three solid fermentation black tea slags in this preferred embodiment are prepared from by following processing technology:
(1) with the black tea slag of 75% and the dish dregs of rice of 8% after autoclaving, the cotton dregs of 9% and 8% wheat bran mixing after, add fermented bacterium, ferment in 1000ml triangular flask; Wherein, black tea slag, the 8% dish dregs of rice, 9% cotton dregs and 8% wheat bran of 75% are mass percent.
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis, short and small spore bacillus and postpones bacillus, and the bacterial concentration of bacillus subtilis is OD
600=0.5-1, the bacterial concentration OD of short and small spore bacillus
600=0.5-1, the bacterial concentration postponing bacillus is OD
600=0.5-1, and bacillus subtilis, short and small spore bacillus with delay bacillus mix with volume ratio 1:2:1 ratio, the fermentate that step (1) obtains and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml, cultivate 3 days at 37 DEG C, carry out one time fermentation, in one time fermentation incubation every 1h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, mixed fungus comprises aspergillus oryzae and aspergillus niger, and aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:1 ratio, every 100g fermentate inoculation 1 × 10
6-1 × 10
8individual mixed fungus.Cultivate 4 days at 2 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2 days at 35 DEG C, carry out three fermentations, in three fermented and cultured processes every 5h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation black tea slags.
Experiment 1:
Employing single factor test contrasts, the egg feedstuff of egg feedstuff and the routine of adding a kind of laying hen premix material of anti heat stress of the present invention is utilized to feed the laying hen 240 of 290 ages in days in summer, Random assignment becomes two groups, be respectively heat stress control group and heat stress experimental group, often organize 120, experiment 6-7 week, average room temperature is 30 DEG C, freely drink water and search for food, egg number, feed intake and egg size is recorded in experimentation, and calculate average egg production, the average egg weight, on average daily ingestion amount, feedstuff-egg ratio of each group of laying hen, specifically see table 1.
Table 1: the performance in layers table of comparisons
Known by table 1, after cycling hot stress process, add heat stress experimental group of the present invention and contrast with the heat stress control group of feeding conventional egg feedstuff, average egg production, average egg weight, average daily ingestion amount, feedstuff-egg ratio obviously improve.Result illustrates, heat stress obviously can reduce production performance of layer chicken, and premix material of anti heat stress can this negative effect of larger alleviation, therefore has good anti-heat stress effect.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. a laying hen premix material of anti heat stress, it is characterized in that, with parts by weight, composed of the following components: glutamine 90-130, citric acid 5-10, fumaric acid 1-2, sodium acetate 2-3, sodium Diacetate 2-3, sodium butyrate 4-5, phenylacetic acid 1-2, Bacitracin Zinc 0.1-0.15, benzoic acid 6-8, yeast selenium 15-30, astaxanthin 2-4, GABA 8-12, oligosaccharides 100-150, three solid fermentation black tea slag 650-800.
2. a kind of laying hen premix material of anti heat stress according to claim 1, it is characterized in that, with parts by weight, composed of the following components: glutamine 130, citric acid 5, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate g, phenylacetic acid g, Bacitracin Zinc 0.13, benzoic acid 8, yeast selenium 30, astaxanthin 3, GABA 10, oligosaccharides 140, three solid fermentation black tea slags 662.87.
3. a kind of laying hen premix material of anti heat stress according to claim 1, it is characterized in that, with parts by weight, composed of the following components: glutamine 125, citric acid 6, fumaric acid 1, sodium acetate 2, sodium Diacetate 2, sodium butyrate 5, phenylacetic acid 2, Bacitracin Zinc 0.15, benzoic acid 7, yeast selenium 20, astaxanthin 4, GABA 11, oligosaccharides 150, three solid fermentation black tea slags 664.85.
4. a preparation method for laying hen premix material of anti heat stress, is characterized in that, comprises preparation three solid fermentation black tea slags, prepares three solid fermentation black tea slags and comprises the steps:
(1) fermentate comprises fermentation major ingredient and fermentation auxiliary material, and described fermentation major ingredient is black tea slag, after being mixed by the black tea slag of 65-80%, adds fermented bacterium, ferments with the fermentation auxiliary material of 20-35%;
(2) one time fermentation: the fermented bacterium that step (1) uses is Mixed Microbes, described Mixed Microbes comprises bacillus subtilis, short and small spore bacillus and postpones Bacillus, bacillus subtilis, short and small spore bacillus and delay Bacillus mix with arbitrary proportion, the mixture that step (1) obtains cultivates 3-4 days at 30-37 DEG C, carry out one time fermentation, in one time fermentation incubation every 1-2h upset once;
(3) secondary fermentation: after one time fermentation terminates, take out the fermentate of step (2), renewed vaccination mixed fungus, described mixed fungus comprises candida utili and aspergillus niger, candida utili and aspergillus niger mix with arbitrary proportion, cultivate 3-5 days at 28-32 DEG C, carry out secondary fermentation, in secondary fermentation incubation every 5-7h upset once;
(4) three fermentations: after secondary fermentation terminates, take out the fermentate of step (3), regulate fermentate pH to be 5-6, renewed vaccination moral formula Lactobacillus lactate subspecies, cultivate 2-3 days at 30-37 DEG C, carry out three fermentations, in three fermented and cultured processes every 5-6h upset once;
(5) dry: after step (4) fermentation ends, the fermentate that baking step (4) obtains, is three solid fermentation black tea slags.
5. according to the arbitrary described a kind of preparation method promoting the resisting stress premix of piglet growth of claim 4, it is characterized in that: described fermentation auxiliary material comprises the dish dregs of rice, cotton dregs and wheat bran, the mass percent of its Chinese food dregs of rice, cotton dregs and wheat bran in fermentate is: dish dregs of rice 7-15%, cotton dregs 7-15% and wheat bran 6-15%.
6. a kind of preparation method promoting the resisting stress premix of piglet growth according to claim 4, is characterized in that: in one time fermentation, and the bacterial concentration of described bacillus subtilis is OD
600=0.5-1, the bacterial concentration OD of described short and small spore bacillus
600=0.5-1, the bacterial concentration of described delay bacillus is OD
600=0.5-1, and described bacillus subtilis, short and small spore bacillus with delay bacillus mix with volume ratio 1:2:1 ratio, fermentate and Mixed Microbes are with the ratio mixed culture fermentation of 100-120g:1-1.05ml.
7. a kind of preparation method promoting the resisting stress premix of piglet growth according to claim 4, is characterized in that: in secondary fermentation, and described mixed aspergillus oryzae and aspergillus niger are with the mixing of volume ratio 1:2 ratio, and every 100g fermentate inoculates 1 × 10
6-1 × 10
8individual mixed fungus.
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| CN106173475A (en) * | 2016-07-15 | 2016-12-07 | 山东和美集团有限公司 | Alleviate mixed feed of broiler heat stress and preparation method thereof |
| CN107467370A (en) * | 2017-10-13 | 2017-12-15 | 无锡正大生物股份有限公司 | A kind of feed feature phagostimulant and preparation method thereof |
| CN110178975A (en) * | 2019-06-24 | 2019-08-30 | 广东省农业科学院动物科学研究所 | A kind of yellow-feather broiler fodder improving Performance and immune function |
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