CN109123169A - A kind of Synbiotics - Google Patents

A kind of Synbiotics Download PDF

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Publication number
CN109123169A
CN109123169A CN201810977646.3A CN201810977646A CN109123169A CN 109123169 A CN109123169 A CN 109123169A CN 201810977646 A CN201810977646 A CN 201810977646A CN 109123169 A CN109123169 A CN 109123169A
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CN
China
Prior art keywords
synbiotics
sporolactobacillus
fructooligosaccharide
inulin
cfu
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CN201810977646.3A
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Chinese (zh)
Inventor
吕存
成艳芬
朱伟云
孙玉亭
胡传炯
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Nanjing Agricultural University
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Nanjing Agricultural University
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Priority to CN201810977646.3A priority Critical patent/CN109123169A/en
Publication of CN109123169A publication Critical patent/CN109123169A/en
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Birds (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Health & Medical Sciences (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of Synbiotics, belong to cultural technique field.A kind of Synbiotics, comprising: clostridium butyricum, Sporolactobacillus S1, inulin and fructooligosaccharide；The Sporolactobacillus S1 is provided by what is separated from chitling road by alimentary canal Microbiological Lab of Agricultural University Of Nanjing；This Synbiotics can be applied in porkling aquaculture industry by way of feeding water, can efficiently improve the average daily gain and average daily gain of weanling pig, reduce grice diarrhoea rate, guaranteed intestine of young pigs health, improved piglet growth performance.

Description

A kind of Synbiotics
Technical field
The invention belongs to cultural technique fields, and in particular to a kind of Synbiotics.
Background technique
Wean is the important link of pig production, is changed by breast milk rich in cereal materials during wean due to daily ration Pellet acute variation is occurred using the intestinal microflora that breast milk is set up as substrate along with the variation of external environment, Cause intestine of young pigs Flora Disturbance, destroy intestine of young pigs microbial barrier, increases the risk of pathogen field planting, cause piglet abdomen It rushes down, seriously affects the growth performance of piglet.Synbiotics is that probiotics and prebiotics are used in combination, common to play the prebiotic work of the two Compound micro-ecological preparation.Existing research shows that Synbiotics has and improves piglet growth performance, promotes intestinal growth, adjusts intestines The functions such as road Bacterial community.The patent of patent No. CN200510038893.X, wean can be improved by disclosing Sporolactobacillus S1 The adding weight and reduction diarrhea rate of porkling, but its adding weight is low, diarrhea rate is higher and mechanism of action is not perfect；By gemma Lactobacillus S1 not yet has been reported that with Synbiotics made of other strains and auxiliary material.Therefore, the present invention passes through research clostridium butyricum, bud Synbiotics made of spore lactobacillus S1, inulin and fructooligosaccharide is inquired into and is closed to the mechanism of action of Intestinal Microflora of Weanling Piglets and metabolism Raw element alleviates intestine of young pigs Flora Disturbance and weanling pig growth mechanism caused by weaning stress.Meanwhile passing through child care phase whole process Using Synbiotics, its optimal use time section is studied, the application for being Synbiotics in breeding production provides theoretical foundation.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of Synbiotics, which is by two kinds of probiotics butyric acid shuttles Bacterium, Sporolactobacillus S1 and two kinds of prebiotics inulin, fructooligosaccharide form by a certain percentage, alleviate the piglet as caused by weaning stress Enteric flora disturbance reduces diarrhea, guarantees intestine of young pigs health.Meanwhile promoting piglet growth performance.
The technical solution adopted by the invention is as follows:
A kind of Synbiotics, comprising: clostridium butyricum, Sporolactobacillus S1, inulin and fructooligosaccharide.
Preferably, the Synbiotics includes: Sporolactobacillus S1 1 × 1010~1 × 1013CFU, clostridium butyricum 3 × 1010~ 3×101315~30g of CFU, 25~40g of inulin and fructooligosaccharide.
Preferably, the Synbiotics includes: Sporolactobacillus S1 1 × 1010~1 × 1011CFU, clostridium butyricum 3 × 1010~ 3×101120~30g of CFU, 25~30g of inulin and fructooligosaccharide.
Preferably, the Synbiotics includes: Sporolactobacillus S1 1 × 1010CFU, clostridium butyricum 3 × 1010CFU, inulin 30g and fructooligosaccharide 20g.
Preferably, the Sporolactobacillus S1 is separated from chitling road.
Specifically, the Sporolactobacillus S1 is provided by alimentary canal Microbiological Lab of Agricultural University Of Nanjing, culture presevation Number be CGMCC NO.1315.
Preferably, pill, tablet, particle and powder can be made in the Synbiotics.
Preferably, the Synbiotics applies in aquaculture industry.
Specifically, Synbiotics of the invention alleviates weaning stress of piglet by the way of feeding water.
By adopting the above technical scheme, the invention has the benefit that Sporolactobacillus S1 separates in Synbiotics of the invention In the excellent chitling road of growth performance, securely and reliably, Growth Performance of Weaning Piglets can be improved, reduce grice diarrhoea rate.Butyric acid Clostridium, inulin and fructooligosaccharide can promote such as Bifidobacterium, the proliferation of the probiotics such as Bacillus acidi lactici, adjust intestinal microflora, subtract The field planting of few pathogen, while improving enteric cavity environment, reach guarantee intestinal health, promotes piglet growth performance.With the prior art It compares, Sporolactobacillus S1 is combined the Synbiotics to be formed with clostridium butyricum, inulin and fructooligosaccharide by the present invention both can be efficient It reduces diarrhea of weaned piglets rate and the average daily feed intake of porkling and adding weight dramatically increases；The faecal lactate content of weaned piglets and The content of short chain fatty acids increases, and intestinal pH reduces, and can inhibit the proliferation of pathogen, short chain fatty acids are beneficial microorganisms Metabolite is conducive to intestinal health；It is fed by the way of feeding water, it is simple and convenient.
Detailed description of the invention
Fig. 1 is the influence that Synbiotics belongs to horizontal relative abundance to child care Pig fecal bacteria.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive Feature and/or step other than, can combine in any way.
Embodiment 1
A kind of Synbiotics: Sporolactobacillus S1 1 × 1010, clostridium butyricum 3 × 1010CFU, inulin 30g and fructooligosaccharide 20g.
Embodiment 2
A kind of Synbiotics: Sporolactobacillus S1 1 × 1013CFU, clostridium butyricum 3 × 1012CFU, inulin 25g and fructooligosaccharide 15g。
Embodiment 3
A kind of Synbiotics: Sporolactobacillus S1 1 × 1011CFU, clostridium butyricum 3 × 1011CFU, inulin 32g and fructooligosaccharide 25g。
Embodiment 4
A kind of Synbiotics: Sporolactobacillus S1 1 × 1012CFU, clostridium butyricum 3 × 1010, inulin 40g and fructooligosaccharide 15g.
Embodiment 5
A kind of Synbiotics: Sporolactobacillus S1 1 × 1011CFU, clostridium butyricum 3 × 1012CFU, inulin 30g and fructooligosaccharide 30g。
Embodiment 6
A kind of Synbiotics is prepared into pill, tablet, particle and powder with embodiment 1.
Embodiment 7
A kind of Synbiotics is prepared into pill, tablet, particle and powder with embodiment 2.
Embodiment 8
A kind of Synbiotics is prepared into pill, tablet, particle and powder with embodiment 3.
Embodiment 9
A kind of Synbiotics is prepared into pill, tablet, particle and powder with embodiment 4.
Embodiment 10
A kind of Synbiotics is prepared into pill, tablet, particle and powder with embodiment 5.
Embodiment 11
Application in porkling aquaculture industry:
A kind of Synbiotics comes from Han Shiwei company with embodiment 1, clostridium butyricum；Sporolactobacillus S1 is big from Nanjing agriculture Chemical digestion road Microbiological Lab；Inulin and fructooligosaccharide come from Beijing Hou Jia Bioisystech Co., Ltd, and the place of production is Chilean, and purity is big In 90%.
This Synbiotics is fed to the same batch health weanling pig of 21 ages in days wean, concrete mode in a manner of feeding water It is as follows:
The same batch health weanling pig that 240 body conditions are close (6.35 ± 0.20kg), and 21 ages in days wean is chosen in test, point It is two groups, control group and processing group, as unit of field, every group is divided into three Duplicate Samples groups, 40 piglets of each Duplicate Samples group. Water of the control group fed without Synbiotics, each field of processing group pass through drinking-water daily and feed above-mentioned Synbiotics, and 21-42 age in days is daily Feeding；42-70 age in days is fed once every other day, and dose is one times of the daily scale of feeding of 21-42 age in days.
This test carries out on the Chinese generation pig farm big Bangbu Wei Zhuan, free water, is freely eaten.Immune and disinfection is in strict accordance with the Chinese The normal flow of Shi Wei company carries out.
Embodiment 12
Microbial bacteria group structure and short chain fatty acids content measuring, specific method are carried out to the raising porkling of embodiment 11 Are as follows: take piglet 21,28,42 and 70 ages in days to acquire control group and test group piglet excrement, -20 DEG C of preservations respectively.
Growth performance: respectively at 21 (before tests), 42,70 ages in days carry out empty stomach weighing to piglet, calculate being averaged for piglet Daily gain.The daily feed intake of piglet is recorded, average daily gain and feed-weight ratio are calculated.The daily diarrhea piglet head of accurate recording Number calculates diarrhea rate.Calculation method is as follows: average daily gain=column total augment weight/(column piglet number × raising number of days)；It is average to adopt day The total feed intake in appetite=column/(column piglet number × raising number of days)；Feed-weight ratio=average daily gain/average daily gain；Diarrhea rate (%)=diarrhea head/(the total head number × raising number of days of examination pig) × 100%, wherein every is denoted as 1 diarrhea in grice diarrhoea 1 day Head.Concrete outcome such as table 1 is shown:
Influence of 1 Synbiotics of table to child care pig growth performance and diarrhea rate
As shown in Table 1, compared with the control group, 21-42 age in days piglet average daily gain is significantly increased using Synbiotics With average daily gain (P < 0.05)；Significantly reduce grice diarrhoea rate (P < 0.05)；It is significantly increased 42-70 days using Synbiotics The average daily gain (P < 0.05) of age piglet, to average daily gain, feed-weight ratio and diarrhea rate are had no significant effect.
Synbiotics of the present invention is fed by feeding water mode, and 21~42 age in days average daily gain amounts are improved than control group 12.31%, diarrhea rate is down to 2.64 ± 0.04%, better than individually hello water Sporolactobacillus S1 (21~42 age in days average daily gains Amount improves 10.22% than control group, and diarrhea rate is down to 2.9 ± 0.01%)；42~70 age in days average daily gain amounts are mentioned than control group High by 20.36%, diarrhea rate is down to 1.54 ± 0.49%, and better than water Sporolactobacillus S1 is individually fed, (21~42 ages in days averagely increase day by day Weight ratio control group improves 17.22%, and diarrhea rate is down to 1.8 ± 0.02%).
Fecal specimens Bacterial community analysis: the extraction of faecal microbiota DNA: take 2ml of the 0.3g fecal specimens after sterilizing from In heart pipe, 1mLPBS buffer is added, after vortex oscillation, 3000 × g is centrifuged 5min, draws upper layer bacterium solution and is transferred to new 2ml In centrifuge tube, after 12000 × g is centrifuged 5min, gives up supernatant, 1mL CTAB solution is added, is transferred to after mixing containing 0.3g After sufficiently oscillation mixes in the sterile zirconium pearl pipe of zirconium pearl, cell wall is destroyed using bead mill formula tissue grinder, is extracted micro- in excrement The total DNA of biology.
The area bacterial 16 S rRNA gene V4 is expanded using bacterial universal primers 515F and 806R, amplified production exists Hiseq2500 platform carries out the sequencing of PE250 both-end (Hua Da gene Co., Ltd).Removal length first is less than after the sequencing is completed 400bp, the sequence greater than 500bp, and the sequence of the base containing N, then splice data and remove average quality and be lower than 30 sequence.Filtered sequence carries out OTU cluster in QIIME.Chao1 index is calculated in flora OTU level, to comment Estimate the richness of intestinal flora, Shannon and Simpson index is calculated, to assess the diversity of intestinal flora.
28,42,70 days intestinal flora diversity and richness the results are shown in Table 2, as shown in Table 2, using Synbiotics to pig manure Just flora richness and diversity have no significant effect.Belong to horizontal upper further analysis, as shown in Figure 1, significant using Synbiotics Increase Lactobacillus (Lactobacillus) and unfiled Ruminococcus section in 28 age in days piglet excrement The relative abundance (P < 0.05) of (unclassified Ruminococcaceae)；It significantly increases and intends bar in 42 age in days swine excrements The relative abundance (P < 0.05) of Pseudomonas (Bacteroides) and general Bordetella (Prevolla)；To 70 age in days Pseudomonas relative abundances Have no significant effect (P > 0.05).
After Synbiotics water of the invention feeds weaned piglets, porkling intestinal flora multiplicity, Lactobacillus, unfiled cud ball Cordycepps, Bacteroides and general Bordetella relative abundance increase, and are conducive to intestinal health.
Influence of 2 Synbiotics of table to child care Pig fecal bacteria diversity and richness
The measurement of excrement Short-Chain Fatty Acids content: taking 0.3g fecal specimens to be added in 2ml centrifuge tube, and it is super that 1.5ml is added Mixing fullys shake in pure water, and 12,000 × g is centrifuged 5min, takes 300 μ l of supernatant, and 25% (w/v) is added according to the ratio of 5:1 Metaphosphoric acid crotons acid solution, -20 DEG C are overnight, then with 12, after 000 × g is centrifuged 10min, Aspirate supernatant, and use 0.22 μm Membrane filtration supernatant after ether extracts filtered supernatant, detects short chain rouge using Agilent 7890A gas chromatograph The concentration of fat acid, as a result as table 3 is shown:
As shown in Table 3, the concentration of total short chain fatty acids and butyric acid in child care phase swine excrement can be dramatically increased using Synbiotics (P<0.05)；Dramatically increase acetic acid concentration (P < 0.05) in 28 and 42 age in days swine excrements；Do not have to child care phase swine excrement propionate concentration Have a significant impact (P > 0.05).
After Synbiotics water of the invention feeds weaned piglets, total short chain fatty acids in pig manure are dramatically increased, and short-chain fat Acid can inhibit the proliferation of pathogen, and short chain fatty acids are beneficial microbial metabolic products；Total short chain fatty acids in swine excrement, Butyric acid and acetic acid concentration increase, and intestinal pH decline is conducive to intestinal health.
Influence (μm ol/g) of 3 Synbiotics of table to piglet faecal short chain fatty acid content
The independent samples t test that data are all made of in 20.0 software of SPSS is analyzed, confidence interval 95%.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed New feature or any new combination, and disclose any new method or process the step of or any new combination.

Claims (7)

1. a kind of Synbiotics characterized by comprising clostridium butyricum, Sporolactobacillus S1, inulin and fructooligosaccharide.
2. Synbiotics as described in claim 1, which is characterized in that the Synbiotics includes: Sporolactobacillus S1 1 × 1010~1 ×1013CFU, clostridium butyricum 3 × 1010~3 × 101315~30g of CFU, 25~40g of inulin and fructooligosaccharide.
3. Synbiotics as described in claim 1, which is characterized in that the Synbiotics includes: Sporolactobacillus S1 1 × 1010~1 ×1011CFU, clostridium butyricum 3 × 1010~3 × 101120~30g of CFU, 25~30g of inulin and fructooligosaccharide.
4. Synbiotics as described in claim 1, which is characterized in that the Synbiotics includes: Sporolactobacillus S1 1 × 1010, fourth Sour clostridium 3 × 1010CFU, inulin 30g and fructooligosaccharide 20g.
5. Synbiotics as described in any one of claims 1 to 4, which is characterized in that the Sporolactobacillus S1 is from chitling Road separation.
6. Synbiotics as described in claim 1, which is characterized in that the Synbiotics can be made pill, tablet, particle and dissipate Agent.
7. Synbiotics as described in claim 1, which is characterized in that the Synbiotics applies in aquaculture industry.
CN201810977646.3A 2018-08-27 2018-08-27 A kind of Synbiotics Pending CN109123169A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1687400A (en) * 2005-04-15 2005-10-26 南京农业大学 Sporolactobacillaceae and produced preparation of living fungus thereof
CN103053806A (en) * 2012-12-27 2013-04-24 深圳华大基因科技有限公司 A synbiotic feed additive, and a preparation method and applications thereof
CN106689672A (en) * 2017-01-04 2017-05-24 陈雨 Synbiotics composition, synbiotics preparation, preparation method and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1687400A (en) * 2005-04-15 2005-10-26 南京农业大学 Sporolactobacillaceae and produced preparation of living fungus thereof
CN103053806A (en) * 2012-12-27 2013-04-24 深圳华大基因科技有限公司 A synbiotic feed additive, and a preparation method and applications thereof
CN106689672A (en) * 2017-01-04 2017-05-24 陈雨 Synbiotics composition, synbiotics preparation, preparation method and application thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
戴兆来等: "合生元组合筛选及对仔猪生产性能和腹泻的影响", 《南京农业大学学报》 *

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Application publication date: 20190104