CN103053806A - A synbiotic feed additive, and a preparation method and applications thereof - Google Patents
A synbiotic feed additive, and a preparation method and applications thereof Download PDFInfo
- Publication number
- CN103053806A CN103053806A CN2012105803750A CN201210580375A CN103053806A CN 103053806 A CN103053806 A CN 103053806A CN 2012105803750 A CN2012105803750 A CN 2012105803750A CN 201210580375 A CN201210580375 A CN 201210580375A CN 103053806 A CN103053806 A CN 103053806A
- Authority
- CN
- China
- Prior art keywords
- weight
- feed additive
- weight portions
- synbiotic
- parts
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The present invention discloses a synbiotic feed additive, and a preparation method and applications thereof. The synbiotic feed additive comprises: 1 ~ 5 parts by weight of, preferably 2-4 parts by weight of, more preferably 3-4 parts by weight of, and most preferably 3.5 parts by weight of at least one selected from chitosan oligosaccharide, chitosan and algal oligosaccharide; 2-10 parts by weight of, preferably 3 to 9 parts by weight of, more preferably 4-8 parts by weight of, and most preferably 5.5 parts by weight of at least one selected from astragalus polysacharide, and lycium barbarum polysaccharides hawthorn flavone; and 100 parts by weight of probiotics composition which is composed of bacillus subtilis, bacillus coagulans and clostridium butyricum at a weight ratio of 1-3:1-3:1-3, preferably 3:3:3, relatively preferably 3:2:2, more preferably 3: 2:1, and most preferably 3:1:1. Compared with existing synbiotic feed additives, the synbiotic feed additive of the present invention is easier to digest and absorb, and can significantly improve animal immunity and animal health, increase feed utilization, lower feed conversion rates, promote reproduction, improve production performance, improve meat quality and improve breeding environment.
Description
Technical field
The present invention relates to technical field of animal, especially field of feed additive technology.Particularly, the present invention relates to Synbiotic feed additive and its preparation method and application.More specifically, the present invention relates to Synbiotic feed additive and preparation method thereof, animal feed and veterinary method.
Background technology
At present, China cultivation industry to high yield, high-quality, efficient, ecological, safe modern breeding way change and evolution in, urgent need can substitute or the novel green of part substitute antibiotics and chemicals, the feed addictive of safety.In recent years, the researcher puts forth effort on probiotics (mainly referring to the useful living bacterium that lives), Chinese traditional herbs and the synergy of functional carbohydrate aspect zooprophylazis health care, production performance raising more, and existing probiotics (mainly referring to the useful living bacterium that lives), Chinese traditional herbs and the functional carbohydrate of adopting prepares the report of feed addictive (can be described as Synbiotic feed additive) at present.
Yet present Synbiotic feed additive still remains to be improved.
Summary of the invention
The present invention is intended to solve at least one of technical problem that exists in the prior art.For this reason, one object of the present invention is to propose a kind ofly can to improve efficiently animal health condition, promotes growth of animal, improves breeding performonce fo animals, and the Synbiotic feed additive that improves breeding environment.
Thus, according to an aspect of the present invention, the invention provides a kind of Synbiotic feed additive.According to embodiments of the invention, this Synbiotic feed additive comprises: 1 ~ 5 weight portion, preferred 2 ~ 4 weight portions, more preferably 3 ~ 4 weight portions, most preferably 3.5 weight portions be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least; 2 ~ 10 weight portions, preferred 3 ~ 9 weight portions, more preferably 4 ~ 8 weight portions, most preferably 5.5 weight portions be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least; And the probiotics composition of 100 weight portions, this probiotics composition is 1 ~ 3:1 ~ 3:1 ~ 3 by weight ratio, preferred 3:3:3, more preferably 3:2:2, more preferably 3:2:1, most preferably the bacillus subtilis of 3:1:1, bacillus coagulans and clostridium butyricum form.The inventor is surprised to find, compare with existing Synbiotic feed additive, Synbiotic feed additive of the present invention is easier to be digested and absorb, and can significantly improve animal immunizing power, improves animal health condition, improves efficiency of feed utilization, reduces feedstuff-meat ratio, promotes reproduction, improves production performance, improves meat, improves breeding environment.
Particularly, according to embodiments of the invention, Synbiotic feed additive of the present invention, take the probiotics composition as main component, can give full play to probio (being probiotics) and improve the gut flora balance, promote that nutrient digestion absorbs, the antagonism pathogenic bacteria breed, the critical function of enhanced machine body immunity function; The chitosan oligosaccharide that is aided with again the 1-5 weight portion all has obvious facilitation to metabolism, the speed of growth, premunition, the meat aspect of livestock and poultry; And the astragalus polyose of interpolation 2-10 weight portion, can be when it brings into play antibiotic, antiviral functions, can also form synergy with probiotics and chitosan oligosaccharide, thus make Synbiotic feed additive of the present invention improve immunity of livestock, improve efficiency of feed utilization, reduce feedstuff-meat ratio, promote reproduction, the effect of improving the aspects such as meat is remarkable.
According to some embodiments of the present invention, Synbiotic feed additive of the present invention comprises: the chitosan oligosaccharide of 5 weight portions; The astragalus polyose of 10 weight portions; And the probiotics composition of 100 weight portions.Thus, Synbiotic feed additive of the present invention improves animal immunizing power, to improve the animal health condition texts more remarkable.
Need to prove, probiotics (is also referred to as " useful viable bacteria " in this article sometimes, " the useful living bacterium that lives " or " probio ") for feed addictive, can help probio growth in the animal body, the breeding of antagonism pathogenic bacteria, the enhanced machine body immunity function, the generation of control and minimizing disease, improve the host health level, improve food conversion ratio, improve breeding performonce fo animals, promote nutrient digestion to absorb, purify the animal body internal and external environment, suppress the growth of putrefactivebacteria, and then reduce the generation of the harmful substances such as ammonia, amine.The inventor finds that bacillus subtilis, bacillus coagulans and clostridium butyricum all are that activity is the strongest, the most significant useful one of the livings bacterium that lives of effect, and the probiotics composition that the three is mixed composition is during for Synbiotic feed additive, and function is more remarkable.Thus, Synbiotics additive of the present invention adopts the probiotics composition that is comprised of bacillus subtilis, bacillus coagulans and clostridium butyricum.Wherein, according to embodiments of the invention, the ratio of bacillus subtilis, bacillus coagulans and clostridium butyricum is not particularly limited in the probiotics composition, as previously mentioned, can be 1 ~ 3:1 ~ 3:1 ~ 3, preferred 3:3:3, more preferably 3:2:2, more preferably 3:2:1, most preferably 3:1:1.According to concrete examples more of the present invention, in Synbiotic feed additive of the present invention, the probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1:1:1 forms by weight ratio.Thus, comprise the feed addictive of this probiotics composition, improve animal immunizing power, to improve the animal health condition texts remarkable.
According to embodiments of the invention, in Synbiotic feed additive of the present invention, every gram viable count of probiotics composition is not particularly limited.According to concrete examples more of the present invention, every gram viable count of probiotics composition is 1,000,000,000-20,000,000,000.Thus, comprise the feed addictive of this probiotics composition, improve animal immunizing power, to improve the animal health condition texts remarkable.
According to a further aspect in the invention, the present invention also provides the preparation method of foregoing Synbiotic feed additive.According to embodiments of the invention, the method comprises: provide 1 ~ 5 weight portion be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, 2 ~ 10 weight portions be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and the probiotics composition of 100 weight portions, wherein this probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1 ~ 3:1 ~ 3:1 ~ 3 forms by weight ratio; And will be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and this probiotics composition mix, in order to obtain this Synbiotic feed additive.According to embodiments of the invention, utilize the method can effectively prepare Synbiotic feed additive of the present invention.
According to embodiments of the invention, in the preparation method of Synbiotic feed additive of the present invention, provide the chitosan oligosaccharide of 5 weight portions, the astragalus polyose of 10 weight portions and the probiotics composition of 100 weight portions.Thus, the Synbiotic feed additive for preparing improves animal immunizing power, to improve the animal health condition texts remarkable.
According to embodiments of the invention, in the preparation method of Synbiotic feed additive of the present invention, the ratio of bacillus subtilis, bacillus coagulans and clostridium butyricum is not particularly limited in the probiotics composition that provides, and as previously mentioned, can be 1 ~ 3:1 ~ 3:1 ~ 3.According to concrete examples more of the present invention, in the preparation method of Synbiotic feed additive of the present invention, the probiotics composition that provides is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1:1:1 forms by weight ratio.Thus, the Synbiotic feed additive for preparing improves animal immunizing power, to improve the animal health condition texts remarkable.
According to embodiments of the invention, in the preparation method of Synbiotic feed additive of the present invention, every gram viable count of the probiotics composition that provides is not particularly limited.According to concrete examples more of the present invention, in the preparation method of Synbiotic feed additive of the present invention, every gram viable count of the probiotics composition that provides is 1,000,000,000-20,000,000,000.Thus, the Synbiotic feed additive for preparing improves animal immunizing power, to improve the animal health condition texts remarkable.
According to another aspect of the invention, the present invention also provides a kind of animal feed.According to embodiments of the invention, this animal feed comprises foregoing Synbiotic feed additive of the present invention.The inventor finds, compare with existing animal feed, utilize animal feed feeding animals of the present invention, can significantly improve animal immunizing power, improve animal health condition, improve efficiency of feed utilization, reduce feedstuff-meat ratio, promote reproduction, improve production performance, improve meat, improve breeding environment.
According to another aspect of the invention, the present invention also provides a kind of veterinary method.According to embodiments of the invention, this veterinary method adopts foregoing animal feed feeding animals of the present invention.According to embodiments of the invention, compare with existing veterinary method, veterinary method of the present invention, can significantly improve animal immunizing power, improve animal health condition, improve efficiency of feed utilization, reduce feedstuff-meat ratio, promote reproduction, improve production performance, improve meat, improve breeding environment, thereby can obtain efficiently high-quality animal product.
Need to prove, Synbiotic feed additive of the present invention and preparation method thereof, the present inventor finishes through arduous creative work and the work of optimization.Additional aspect of the present invention and advantage in the following description part provide, and part will become obviously from the following description, or recognize by practice of the present invention.
The specific embodiment
Below in conjunction with embodiment the solution of the present invention is made an explanation.It will be understood to those of skill in the art that the following examples only are used for explanation the present invention, and should not be considered as limiting scope of the present invention.Unreceipted concrete technology or condition among the embodiment are carried out according to the document in this area described technology or condition or according to product description.The unreceipted person of production firm of agents useful for same or instrument, being can be by the conventional products of commercial acquisition.
Conventional method:
The method for preparing the Synbiotics additive of the present invention generally comprises following steps:
At first, provide 1 ~ 5 weight portion be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, 2 ~ 10 weight portions be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and the probiotics composition of 100 weight portions, wherein this probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1 ~ 3:1 ~ 3:1 ~ 3 forms by weight ratio.
Then, with above-mentioned be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and this probiotics composition mix, in order to obtain this Synbiotic feed additive.
Embodiment 1
Material and reagent: chitosan oligosaccharide (Dalian Glycobio Co., Ltd, MP103), astragalus polyose (Xianyang aviation one six eight bio-engineering corporations, HK-169), bacillus coagulans bacterial classification (Si Kaida biotechnology (Kunshan) ningjie001 of Co., Ltd), clostridium butyricum bacterial classification (Su Kehan (Weifang) bioengineering Co., Ltd, SUKAFEED-B.Sub), Bacillus subtilis strain (being purchased from Chinese industrial microorganism fungus kind preservation center (Clostridium butyricum CICC20036)).
Equipment:
1, seeding tank (GYS-500C);
2, fermentation tank (GYS-1500C);
3, fermented and cultured case (SPX-1500);
4, stir disintegrating machine (SWFL82D);
5, horizontal pressure steam sterilizer (ZY.01.001);
6, Highspeedcentrifugingandsprayingdrier (LPG1000);
7, thermal shrinking package machine (SM-4525L);
8, quantitative powder filling machine (DXDCF2000A).
At first, according to solid fermentation method, prepare respectively the viable bacteria dry powder formulations of bacillus subtilis, bacillus coagulans and clostridium butyricum, concrete steps are as follows:
1, bacterial classification is prepared
(1) actication of culture
Room temperature is placed bacterial classification glycerine cold storage pipe, treats that this cold storage liquid in pipe all melts, open it on the flame next door, and with the pipettor of 200 microlitres that bacterial classification glycerine cold storage liquid in pipe pressure-vaccum is even.Then, inside the Pass the uniform cold storage of pressure-vaccum, draw 200 microlitre liquid with pipettor, be seeded in the fresh LB fluid nutrient medium of 50ml volume, and on 37 ℃, 160r/min shaking table, carry out activation culture 24h, in order to obtain the bacterial classification through overactivation.
(2) enlarge cultivation
Above-mentioned bacterial classification through overactivation is inoculated in the fresh LB culture medium according to 3% ratio, and on 37 ℃, 160r/min shaking table, enlarges and cultivate 24h, in order to obtain the bacterial classification cultivated through enlarging.
2, solid fermentation
(1) preparation of solid fermentation culture medium and packing
Take by weighing an amount of wheat bran, and add entry according to the mass ratio of 1:1-2 it is stirred, then mixture was sterilized 15-20 minute under 115 ℃ of high temperature, in order to obtain the solid fermentation culture medium.Then, on operating desk, the solid fermentation culture medium that is not less than 70 ℃ is sub-packed in through in the clean fermentation dish of sterilizing, and pinches loose with assembling agglomerating wheat bran in the solid fermentation culture medium.
(2) inoculation
Above-mentioned process is enlarged the bacterial classification of cultivation according to the inoculum concentration of 1-5%, be inoculated in each fermentation dish, and bacterial classification and solid fermentation culture medium are are fully stirred and evenly mixed, naturally pave, then under 37 ℃, fermented 48 hours, in order to obtain fermenting wheat bran.
3, fermenting wheat bran is pulverized
The fermenting wheat bran of above-mentioned acquisition is placed drying box, and then 60 ℃ of constant temperature drying 12-16h, utilize 100 mesh sieves to pulverize through the fermenting wheat bran of oven dry, in order to obtain the viable bacteria dry powder formulations, pack is preserved.Wherein, leave and take sample segment and carry out count plate, it is for subsequent use to choose every gram viable count and be 1,000,000,000-20,000,000,000 viable bacteria dry powder formulations.
Thus, prepare respectively the viable bacteria dry powder formulations of bacillus subtilis, bacillus coagulans and clostridium butyricum.
Then, with reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, mix according to the ratio of the weight ratio 3:3:3 viable bacteria dry powder formulations with the above-mentioned bacillus subtilis for preparing, bacillus coagulans and clostridium butyricum bacillus subtilis, bacillus coagulans and clostridium butyricum, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 5 weight portions, the astragalus polyose of 10 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 2
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:2:2, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 1 weight portion, the astragalus polyose of 2 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 3
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:2:1, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 2 weight portions, the astragalus polyose of 3 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 4
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:1:1, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 4 weight portions, the astragalus polyose of 9 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 5
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:1:1, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 3 weight portions, the astragalus polyose of 4 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 6
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:1:1, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 4 weight portions, the astragalus polyose of 8 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 7
With reference to aforementioned conventional method, prepare Synbiotic feed additive of the present invention according to following steps:
At first, according to the ratio of weight ratio 3:1:1, the viable bacteria dry powder formulations of the bacillus subtilis, bacillus coagulans and the clostridium butyricum that prepare among the embodiment 1 is mixed, in order to obtain the probiotics composition.
Then, the chitosan oligosaccharide of 3.5 weight portions, the astragalus polyose of 5.5 weight portions and the above-mentioned probiotics composition of 100 weight portions are mixed, in order to obtain Synbiotic feed additive of the present invention.
Embodiment 8 Synbiotic feed additives are to the efficacy test of piglet
Take the patent publication No. as contrast (being called in the present embodiment " contrast Synbiotic feed additive ") as the Synbiotic feed additive described in the patent of CN 100589703C, embodiment 1-7 is prepared Synbiotic feed additive the effect performance of piglet is estimated, concrete grammar is as follows:
At first, choose 1800 of the weanling pigs that average weight is about 7.8 kilograms, and be divided at random 9 groups: 7 experimental group, 1 control group and 1 blank group.
Then, in feed, add the Synbiotic feed additive that embodiment 1-7 prepares respectively according to the weight ratio of 0.1-1%, in order to prepare respectively 7 kinds of feeds of the present invention; Weight ratio according to 0.1%-1% is added the contrast Synbiotic feed additive in feed, in order to prepare control feed.
Then, each piglet is carried out flat the supporting in ground raise 28 days (being the child care phase), then, go to and fatten the hurdle.Wherein, each experimental group feed of the present invention (it is number corresponding by the Preparation Example of the Synbiotic feed additive that is added in the feeding feed stuff with it respectively wherein to test group number) of feeding respectively, the control group fed control feed, the blank group non-additive feed of feeding, all day is fed free choice feeding 2 times to each group.Then, statistics is respectively organized initial number, gross weight and the counterpoise of piglet respectively, a dead number and gross weight, and finally go to a number, gross weight and the counterpoise of fattening the hurdle, and calculate the omnidistance net gain of respectively organizing piglet, total feed consumption, daily gain, feedstuff-meat ratio and the death rate.The results are shown in following table 1.
Wherein, the contrast Synbiotic feed additive is to be that the embodiment 1 described preparation method of the patent of CN 100589703C prepares according to the patent publication No..Particularly, it is 50,000,000,000 bacillus subtilis dry powder (Su Kehan (Weifang) bioengineering Co., Ltd with every gram viable count, SUKAFEED-B.Sub) and every gram viable count be 20,000,000,000 bacillus licheniformis dry powder (Su Kehan (Weifang) bioengineering Co., Ltd, SUKAFEED-B.Sub), mix by weight 2:1, obtain the probiotics composition, then this probiotics composition and astragalus polyose, xylo-oligosaccharide and silica are mixed by the weight ratio of looking for 3:0.5:0.02:1, make this contrast Synbiotic feed additive.
Table 1
Annotate: in the test statistics data, weight is accurate to 1kg, daily gain, feedstuff-meat ratio, the death rate and is accurate to behind the decimal point 2.
As seen from the above table, each experimental group is maximum dead 2 piglets within the child care phase in 4 weeks, piglet daily gain 0.43-0.49kg, the feedstuff-meat ratio 1.80-1.86 of survival; Dead 5 piglets of control group, residue piglet daily gain 0.38kg, feedstuff-meat ratio are 2.00; The death of blank group reaches 12, and residue piglet daily gain 0.33kg, feedstuff-meat ratio are 2.20.
Above result shows, compares with control group and blank group, and the indexs such as the survival rate of the weanling pig of each experimental group, daily gain, feedstuff-meat ratio are significantly increased.Prove that thus with respect to existing Synbiotic feed additive, Synbiotic feed additive of the present invention can significantly improve pigling immunity, improve efficiency of feed utilization, reduce feedstuff-meat ratio.
Embodiment 9 Synbiotic feed additives are to the efficacy test of sow
Take the contrast Synbiotic feed additive described in the embodiment 8 as contrast, the Synbiotic feed additive of the present invention that embodiment 1-7 prepares to be estimated the effect performance of sow, concrete grammar is as follows:
At first, choose 144 of sows, and be divided at random 9 groups: 7 experimental group, 1 control group and 1 blank group.
Then, treat to begin to feed when respectively organizing sow changes obstetric table over to piglet birth wean in 28 days, day feeds 3 times, and free choice feeding take the material of cannot having enough surplusly as principle, and is taked whole day water supply way, lets alone free drinking-water; The piglet feed lactation pig feed of 20-28 day, day feeds 2 times, free choice feeding; Clear up ight soil every day, sweep pig house once, and observe at any time and respectively organize growth and the health condition of piglet, no matter be milking sow or piglet, diseases, in time treatment.Wherein, each experimental group feed of the present invention described in the embodiment 8 (it is number corresponding by the Preparation Example of the Synbiotic feed additive that is added in the feeding feed stuff with it respectively wherein to test group number) of feeding respectively, control feed described in the control group fed embodiment 8, the blank group non-additive feed of feeding.Then, respectively statistics respectively organize sow initial number and day lactation feed consumption, be born and survive piglet quantity, 21 age in days weanling pig weight, 28 age in days piglets turn nursing cote weight, and calculate piglet survival ratio of each group.The results are shown in following table 2.
Table 2
As seen from the above table, compare with control group and blank group, the piglet survival ratio of each experimental group is significantly increased, and weanling pig, the body weight that turns the nursing cote piglet obviously improve, and sow production performance all has larger improvement.Show that thus with respect to existing Synbiotic feed additive, Synbiotic feed additive of the present invention can significantly improve sow production performance.
In the description of this specification, the description of reference term " embodiment ", " some embodiment ", " example ", " concrete example " or " some examples " etc. means to be contained at least one embodiment of the present invention or the example in conjunction with specific features, structure, material or the characteristics of this embodiment or example description.In this manual, the schematic statement of above-mentioned term not necessarily referred to identical embodiment or example.And the specific features of description, structure, material or characteristics can be with suitable mode combinations in any one or more embodiment or example.
Although illustrated and described embodiments of the invention, those having ordinary skill in the art will appreciate that: can carry out multiple variation, modification, replacement and modification to these embodiment in the situation that does not break away from principle of the present invention and aim, scope of the present invention is limited by claim and equivalent thereof.
Claims (10)
1. a Synbiotic feed additive is characterized in that, comprising:
1 ~ 5 weight portion, preferred 2 ~ 4 weight portions, more preferably 3 ~ 4 weight portions, most preferably 3.5 weight portions be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least;
2 ~ 10 weight portions, preferred 3 ~ 9 weight portions, more preferably 4 ~ 8 weight portions, most preferably 5.5 weight portions be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least; And
The probiotics composition of 100 weight portions, described probiotics composition is 1 ~ 3:1 ~ 3:1 ~ 3 by weight ratio, preferred 3:3:3,3:2:2 more preferably, more preferably 3:2:1, most preferably the bacillus subtilis of 3:1:1, bacillus subtilis, bacillus coagulans and clostridium butyricum clostridium butyricum form.
2. Synbiotic feed additive according to claim 1 is characterized in that, comprising:
The chitosan oligosaccharide of 5 weight portions;
The astragalus polyose of 10 weight portions; And
The probiotics composition of 100 weight portions.
3. Synbiotic feed additive according to claim 1 is characterized in that,
Described probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1:1:1 forms by weight ratio.
4. Synbiotic feed additive according to claim 1 is characterized in that,
Every gram viable count of described probiotics composition is 1,000,000,000-20,000,000,000.
5. the preparation method of Synbiotic feed additive claimed in claim 1 is characterized in that, comprising:
Provide 1 ~ 5 weight portion be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, 2 ~ 10 weight portions be selected from astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and the probiotics composition of 100 weight portions, wherein said probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1 ~ 3:1 ~ 3:1 ~ 3 forms by weight ratio; And
With described be selected from chitosan oligosaccharide, shitosan and alga oligosaccharides one of at least, described astragalus polyose, LBP-X and flavone of hawthorn fruit one of at least and described probiotics composition mix, in order to obtain described Synbiotic feed additive.
6. method according to claim 5 is characterized in that,
The chitosan oligosaccharide of 5 weight portions, the astragalus polyose of 10 weight portions and the probiotics composition of 100 weight portions are provided.
7. method according to claim 5 is characterized in that,
Described probiotics composition is that bacillus subtilis, bacillus coagulans and the clostridium butyricum of 1:1:1 forms by weight ratio.
8. method according to claim 5 is characterized in that,
Every gram viable count of described probiotics composition is 1,000,000,000-20,000,000,000.
9. an animal feed is characterized in that, comprises the described Synbiotic feed additive of claim 1-4.
10. a veterinary method is characterized in that, adopts the animal feed claimed in claim 9 described animal of feeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105803750A CN103053806A (en) | 2012-12-27 | 2012-12-27 | A synbiotic feed additive, and a preparation method and applications thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012105803750A CN103053806A (en) | 2012-12-27 | 2012-12-27 | A synbiotic feed additive, and a preparation method and applications thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103053806A true CN103053806A (en) | 2013-04-24 |
Family
ID=48096952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012105803750A Pending CN103053806A (en) | 2012-12-27 | 2012-12-27 | A synbiotic feed additive, and a preparation method and applications thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103053806A (en) |
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103349153A (en) * | 2013-08-07 | 2013-10-16 | 王春 | Thallus compound with animal intestine repairing function and production method of complex |
| CN103609898A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of composite immunopotentiator for pseudosciaena crocea |
| CN103689266A (en) * | 2013-12-07 | 2014-04-02 | 青岛海芬海洋生物科技有限公司 | Pet feed additive containing marine organisms |
| CN104115995A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院大连化学物理研究所 | Composite carbohydrate preparation for improving animal semen vitality and application thereof |
| CN104171421A (en) * | 2014-08-08 | 2014-12-03 | 河南牧翔动物药业有限公司 | Feed additive for conditioning intestines of animals |
| CN104543389A (en) * | 2014-12-25 | 2015-04-29 | 青岛颂田生物技术有限公司 | Efficient green chitosan oligosaccharide compound agricultural additive |
| CN105533167A (en) * | 2015-12-08 | 2016-05-04 | 福建省农业科学院中心实验室 | Alga oligosaccharide fish feed additive |
| CN106578453A (en) * | 2016-11-28 | 2017-04-26 | 宁夏农林科学院 | Sheep immunopotentiation functional additive, preparation method and application thereof |
| CN106616233A (en) * | 2016-12-30 | 2017-05-10 | 深圳市前海金卓生物技术有限公司 | Premixed feed for degradating mycotoxin, preparation method thereof and pig daily diet |
| CN106721144A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | A kind of beef cattle immunopotentiation multifunctional and nutritional adjusting control agent and its technique and application |
| CN106721254A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | The health additive and its technique of one boar and application |
| CN106721141A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | A kind of milk cow immunopotentiation functional additive and preparation method and application |
| CN106974068A (en) * | 2017-03-23 | 2017-07-25 | 陕西金冠牧业有限公司 | A kind of Chinese medicine compound micro-ecological preparation for improving quail immunity |
| CN109123169A (en) * | 2018-08-27 | 2019-01-04 | 南京农业大学 | A kind of Synbiotics |
| CN110353119A (en) * | 2019-08-23 | 2019-10-22 | 青岛琛蓝医药科技发展有限公司 | A kind of chitosan oligosaccharide probiotics compound and its application with the domestic black pig meat quality flavor of improvement |
| CN115736112A (en) * | 2022-11-15 | 2023-03-07 | 西北农林科技大学 | Feed additive for reducing diarrhea rate of lactating calves and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN1969657A (en) * | 2005-11-25 | 2007-05-30 | 重庆市畜牧科学研究院 | Probiotic feed additive used for pig |
| CN101120726A (en) * | 2007-09-13 | 2008-02-13 | 武汉工业学院 | Synbiotic feed additive |
| CN101904417A (en) * | 2010-07-22 | 2010-12-08 | 浙江工商大学 | Antibiotic complex aquatic feed additive and mixed feed thereof |
-
2012
- 2012-12-27 CN CN2012105803750A patent/CN103053806A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN1969657A (en) * | 2005-11-25 | 2007-05-30 | 重庆市畜牧科学研究院 | Probiotic feed additive used for pig |
| CN101120726A (en) * | 2007-09-13 | 2008-02-13 | 武汉工业学院 | Synbiotic feed additive |
| CN101904417A (en) * | 2010-07-22 | 2010-12-08 | 浙江工商大学 | Antibiotic complex aquatic feed additive and mixed feed thereof |
Non-Patent Citations (3)
| Title |
|---|
| 农业部《新编鱼药手册》编撰委员会: "《新编鱼药手册》", 31 July 2005, article "新编鱼药手册" * |
| 曹晓敏等: "芽孢杆菌作为微生物饲料添加剂的研究和应用", 《养殖技术顾问》, no. 4, 31 December 2009 (2009-12-31) * |
| 王改琴等: "中草药饲料添加剂的功能及其在蛋鸡生产中的应用", 《饲料博览 技术版》, no. 10, 31 December 2010 (2010-12-31) * |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104115995A (en) * | 2013-04-26 | 2014-10-29 | 中国科学院大连化学物理研究所 | Composite carbohydrate preparation for improving animal semen vitality and application thereof |
| CN104115995B (en) * | 2013-04-26 | 2016-06-22 | 中国科学院大连化学物理研究所 | A kind of glycoconjugates preparation improving animal semen vigor and application thereof |
| CN103349153A (en) * | 2013-08-07 | 2013-10-16 | 王春 | Thallus compound with animal intestine repairing function and production method of complex |
| CN103609898A (en) * | 2013-11-26 | 2014-03-05 | 青岛嘉瑞生物技术有限公司 | Preparation method and application of composite immunopotentiator for pseudosciaena crocea |
| CN103689266A (en) * | 2013-12-07 | 2014-04-02 | 青岛海芬海洋生物科技有限公司 | Pet feed additive containing marine organisms |
| CN104171421A (en) * | 2014-08-08 | 2014-12-03 | 河南牧翔动物药业有限公司 | Feed additive for conditioning intestines of animals |
| CN104543389A (en) * | 2014-12-25 | 2015-04-29 | 青岛颂田生物技术有限公司 | Efficient green chitosan oligosaccharide compound agricultural additive |
| CN105533167A (en) * | 2015-12-08 | 2016-05-04 | 福建省农业科学院中心实验室 | Alga oligosaccharide fish feed additive |
| CN106578453A (en) * | 2016-11-28 | 2017-04-26 | 宁夏农林科学院 | Sheep immunopotentiation functional additive, preparation method and application thereof |
| CN106721144A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | A kind of beef cattle immunopotentiation multifunctional and nutritional adjusting control agent and its technique and application |
| CN106721254A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | The health additive and its technique of one boar and application |
| CN106721141A (en) * | 2016-11-28 | 2017-05-31 | 宁夏农林科学院 | A kind of milk cow immunopotentiation functional additive and preparation method and application |
| CN106616233A (en) * | 2016-12-30 | 2017-05-10 | 深圳市前海金卓生物技术有限公司 | Premixed feed for degradating mycotoxin, preparation method thereof and pig daily diet |
| CN106974068A (en) * | 2017-03-23 | 2017-07-25 | 陕西金冠牧业有限公司 | A kind of Chinese medicine compound micro-ecological preparation for improving quail immunity |
| CN109123169A (en) * | 2018-08-27 | 2019-01-04 | 南京农业大学 | A kind of Synbiotics |
| CN110353119A (en) * | 2019-08-23 | 2019-10-22 | 青岛琛蓝医药科技发展有限公司 | A kind of chitosan oligosaccharide probiotics compound and its application with the domestic black pig meat quality flavor of improvement |
| CN115736112A (en) * | 2022-11-15 | 2023-03-07 | 西北农林科技大学 | Feed additive for reducing diarrhea rate of lactating calves and preparation method thereof |
| CN115736112B (en) * | 2022-11-15 | 2024-05-14 | 西北农林科技大学 | Feed additive for reducing diarrhea rate of lactating calves and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103053806A (en) | A synbiotic feed additive, and a preparation method and applications thereof | |
| CN102559545B (en) | Composite micro-ecological preparation for aquatic product and premix thereof | |
| CN103275907B (en) | Bacillus amyloliquefacien and preparation method and application thereof | |
| CN101711548B (en) | Fermented feed containing sodium humate and process and application thereof | |
| CN105010154B (en) | The technique that a kind of recycling sheep manure prepares fermenting bed padding | |
| CN103829042B (en) | A kind of production method of polyvitmin active protein cassava feed | |
| CN104082345A (en) | Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder | |
| CN104161210A (en) | Feed raw material for pregnant sows, preparation method and feed thereof | |
| CN106615658A (en) | Method for preparing fermentation bed padding by recycling froggrass | |
| CN103583880A (en) | Microbial fermentation feed for litopenaeus vannamei | |
| CN102696865A (en) | Method for producing probiotic bacteria granular feed by fermenting corn straw with mixed strain | |
| CN102715351A (en) | Method for producing black pig feed | |
| CN104585510B (en) | A kind of mixed fungus fermentation thing feed addictive and preparation method thereof | |
| CN101756024A (en) | Preparation method of probiotic feed additive | |
| CN108094686A (en) | The compound formulation and preparation method of aquatic livestock disease resistance can be effectively improved | |
| CN103098980A (en) | Microorganism straw feed fermenting agent | |
| CN105533134A (en) | Preparation method of fermentation feed | |
| CN102154145A (en) | Microorganism environment modifier and microorganism packing material | |
| CN104397324B (en) | A kind of Synbiotic feed additive and its application | |
| CN101874812B (en) | Composite bacteria preparation and application thereof in weight increment of immature livestock and control of diarrhea | |
| CN105285327A (en) | Selenium-enriched lactic acid bacterium biological feed additive and preparation method thereof | |
| CN102342371A (en) | Process for preparing compound feed additive from bacillus subtilis natto and bacillus licheniformis | |
| CN104206737A (en) | Safe and efficient biological feed additive | |
| CN106509342A (en) | Feed additive capable of replacing antibiotics and preparation method thereof | |
| CN103205377B (en) | Quality bacillus coagulans for breeding broilers and application of excellent bacillus coagulans |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130424 |