CN103609898A - Preparation method and application of composite immunopotentiator for pseudosciaena crocea - Google Patents
Preparation method and application of composite immunopotentiator for pseudosciaena crocea Download PDFInfo
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- CN103609898A CN103609898A CN201310603264.1A CN201310603264A CN103609898A CN 103609898 A CN103609898 A CN 103609898A CN 201310603264 A CN201310603264 A CN 201310603264A CN 103609898 A CN103609898 A CN 103609898A
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Abstract
The invention discloses an immunopotentiator for pseudosciaena crocea. The immunopotentiator simultaneously has the double effects of a Chinese herbal immunopotentiator and an oligosaccharide immunopotentiator and has the advantages that the addition amount is small, the immunity and the disease resistance of an adult pseudosciaena crocea can be remarkably improved, and the breeding survival rate can be increased by over 10%. The immunopotentiator is prepared by mixing a compound Chinese herbal medicine extract, seaweed oligosaccharide, sulfated lipopolysaccharide, mannan oligosaccharide, vitamin E and sulphate according to a certain proportion, wherein the compound Chinese herbal medicine extract is prepared from Chinese herbal medicines such as radix scutellariae, honeysuckle, rhizoma curculiginis, flatstem milkvetch seeds, radix rehmanniae preparata and the like through an ultrasonic-assisted two-step extraction method. The immunopotentiator simultaneously has the compound immunoenhancement effects of the Chinese herbal immunopotentiator and the oligosaccharide immunopotentiator; if 0.3-0.5% of the immunopotentiator is added in a feed for breeding the pseudosciaena crocea, the immunity of the organism of the pseudosciaena crocea can be effectively enhanced, the breeding risks in a pseudosciaena crocea breeding process are effectively avoided, and no adverse effect for the tastiness of the feed are caused; and the immunopotentiator is free of pollution, residues and side effects, safe and environment-friendly.
Description
Technical field
The present invention relates to preparation method and the application of composite immune reinforcing agent for a kind of large yellow croaker, belong to feed additive field.
Background technology
Aquatic products more and more occupy important share in people's diet, in order to meet the demand of ever-increasing aquatic products, solution becomes the Important Problems of aquaculture because the physiological function that aquaculture scale constantly expands and growth cycle reduction causes is disorderly, disease takes place frequently, and is also the key of aquatic feeds industry development.Under this background, antibiotics feed addictive is subject to heat and holds in both hands, and is widely used in feed industry, has promoted to a certain extent the development of culture fishery, but more serious of the problems such as the high residue that antibiotic brings, high pollution and the resistance to the action of a drug.Current social, food security Frequent Accidents, people also more focus on healthy diet in satisfied having enough to eat and wear, and the aquatic products of green health receive much concern.Chinese herbal feed additive meets the requirement of green aquatic products aquaculture development, has eliminated the safety worries of people to its aquatic products product of feeding out, should vigorously promote the use.But the research level of current Chinese herbal feed additive is lower, manufacture craft simple coarse, the technological element of a product is lower, and quality is stable not, and problem like that is seriously restricting applying of Chinese medicine fodder interpolation.
Large yellow croaker is to be distributed in Middle of The Huanghai Sea to reach the about 60m isobath of China coast to the east of Qiongzhou Strait in the south with the sciaenid fishes germ plasm resource of important component part ，Ye Shi China preciousness of interior distinctive region seawater fish ，Shi China seawater fish fauna.Large yellow croaker has been that one of the large advantage outlet of Marine Cage-culture Fishes and China 8 aquaculture product of national maximum-norm ，Ye Shi Fujian Province and even China show unique characteristics and the best-selling seawater fish in domestic market.In recent years, large yellow croaker aquaculture develops rapidly in China's Coastal Areas, and ，Yi China has formed annual output large yellow croaker 8. 6 * 10 at present
4t, the direct large yellow croaker aquaculture industry of approximately 6,500,000,000 yuan of the output values.But large yellow croaker is after China propagates artificially successfully at present, also exist that larger cultivation risk, particularly culture diseases take place frequently, to cultivate survival rate lower, strengthening large yellow croaker immunocompetence becomes and reduces cultivation risk, improve cultivation income, promote the key of large yellow croaker aquaculture sustainable development.
Summary of the invention
The object of the invention is to according to above shortcomings in prior art, a kind of immunopotentiator using for large yellow croaker is provided, this immunopotentiator has the dual left and right of Chinese medicine immunity enhancer and oligosaccharides immunopotentiator concurrently, there is addition few, can obviously improve immunity and the disease resistance of large yellow croaker adult fish, cultivation survival rate can improve 10% with first-class advantage.
Above-mentioned purpose of the present invention is achieved by the following technical programs:
A large yellow croaker composite immune reinforcing agent, is characterized in that: this immunopotentiator is that the raw material by following mass component mixes: 50～60 parts of compound Chinese herb extracts, 13～17 parts of alga oligosaccharides, 8～12 parts of sulfuric acid ester polysaccharide, 12～18 parts of manna oligosacchrides, 3～7 parts of vitamin E sulfuric esters.
The above-mentioned compound Chinese herb extract of the present invention is dry making after being mixed according to the ratio of 1:1 with corncob by compound Chinese herb leaching liquor.
Above-mentioned Chinese herbal medicine leaching liquor, is comprised of the Chinese herbal medicine of following mass fraction: 10～20 parts of Huang Ling, 8～16 parts of honeysuckles, 8～12 parts of thizoma curculiginises, 4～6 parts of SEMEN ASTRAGALI COMPLANATIs, 4～6 parts, cultivated land, 4～8 parts of glossy ganodermas, 3～5 parts of kuh-sengs, 4～6 parts of China trees, 8～12 parts of milk thistles, 6～10 parts of sweet wormwoods, 4～8 parts of cordate houttuynias, 4～6 parts of Radix Angelicae Sinensis, 3～5 parts of the coptiss, 2～4 parts of polygala roots, 1～3 part of Radix Codonopsis.
In the present invention, said herbal medicine leaching liquor is achieved through the following channels: the impurity in each traditional Chinese medicinal material raw materials is rejected, be chopped into 2 ~ 3mm; According to formula for a product, accurately weigh each raw material; With mobile drinking water, clean traditional Chinese medicinal material raw materials 1～2 time; By leaching liquor pure water in mass ratio the ratio of 30～50:1 join in herbal raw material, under ultrasonic wave booster action, keep slight boiling condition 30min, cross 400 order filter clothes and get juice, filter residue adds 15 times of pure water, by same method lixiviate and mistake leaching juice, merges extracted twice liquid and is leaching liquor.
The invention provides a kind of large yellow croaker composite immune reinforcing agent, it is characterized in that realizing by following approach: compound Chinese herb extract, alga oligosaccharides, sulfuric acid ester polysaccharide, manna oligosacchride, vitamin E sulfuric ester are mixed to rear pulverizing in proportion and evenly make.
The invention provides a kind of large yellow croaker composite immune reinforcing agent, while adding 0.3%～0.5% in large yellow croaker breeding feed, can effectively strengthen the immunity of organisms of large yellow croaker, the generation effectively cultivating in prevention large yellow croaker breeding process, and to the palatability of feed without any harmful effect; Product is for rear pollution-free, the noresidue, without any side effects of large yellow croaker cultivation, safety and environmental protection.
The invention provides a kind of large yellow croaker composite immune reinforcing agent, it is advantageous that: the one, Chinese medicine immunity enhancer and oligosaccharide kind immunopotentiator are used in conjunction with, have the compound raising immunization of Chinese medicine immunity enhancer and oligosaccharide kind immunopotentiator concurrently; The 2nd, and add and compare after the simple pulverizing of current Chinese herbal feed additive, the present invention adopts the mode of adding again after Chinese herbal medicine lixiviate, and short because of large yellow croaker alimentary canal, product of the present invention more can play and improve immune effect; The 3rd, the present invention passes through " ultrasonic wave is assisted two step extractions " and extracts Chinese herbal medicine functional components, has further improved Chinese herbal feed additive functional that strengthens large yellow croaker immunity.
The specific embodiment
embodiment 1:
A large yellow croaker composite immune reinforcing agent, concrete preparation process is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, be chopped into 2 ~ 3mm;
(2) weigh: according to the following formula for a product of gynostemma pentaphylla herbal health care cold tea, accurately weigh 11 parts of each raw material: Huang Ling, 16 parts of honeysuckles, 8 parts of thizoma curculiginises, 6 parts of SEMEN ASTRAGALI COMPLANATIs, 4 parts, cultivated land, 8 parts of glossy ganodermas, 3 parts of kuh-sengs, 6 parts of China trees, 8 parts of milk thistles, 10 parts of sweet wormwoods, 4 parts of cordate houttuynias, 6 parts of Radix Angelicae Sinensis, 3 parts of the coptiss, 4 parts of polygala roots, 3 parts of Radix Codonopsis;
(4) extract: by leaching liquor pure water in mass ratio the ratio of 30:1 join in herbal raw material, under ultrasonic wave booster action, keep slight boiling condition 30min, cross 400 order filter clothes and get juice, filter residue adds 15 times of pure water, press same method lixiviate and cross leaching juice, merge extracted twice liquid and be leaching liquor;
(5) batching: add maize cob meal and stir in 1:1 ratio in leaching liquor;
(6) dry: above-mentioned batching is dried and made compound Chinese herb extract;
(7) composite: compound Chinese herb extract, alga oligosaccharides, sulfuric acid ester polysaccharide, manna oligosacchride, vitamin E sulfuric ester are mixed in following ratio: 50 parts of compound Chinese herb extracts, 17 parts of alga oligosaccharides, 8 parts of sulfuric acid ester polysaccharide, 18 parts of manna oligosacchrides, 7 parts of vitamin E sulfuric esters;
(8) pulverize: the composite immune reinforcing agent ultramicro grinding mixing is crossed to 100 mesh sieves.
embodiment 2:
A large yellow croaker composite immune reinforcing agent, concrete preparation process is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, be chopped into 2 ~ 3mm;
(2) weigh: according to the following formula for a product of gynostemma pentaphylla herbal health care cold tea, accurately weigh 20 parts of each raw material: Huang Ling, 8 parts of honeysuckles, 12 parts of thizoma curculiginises, 4 parts of SEMEN ASTRAGALI COMPLANATIs, 6 parts, cultivated land, 4 parts of glossy ganodermas, 5 parts of kuh-sengs, 4 parts of China trees, 11 parts of milk thistles, 6 parts of sweet wormwoods, 8 parts of cordate houttuynias, 4 parts of Radix Angelicae Sinensis, 5 parts of the coptiss, 2 parts of polygala roots, 1 part of Radix Codonopsis;
(4) extract: by leaching liquor pure water in mass ratio the ratio of 40:1 join in herbal raw material, under ultrasonic wave booster action, keep slight boiling condition 30min, cross 400 order filter clothes and get juice, filter residue adds 15 times of pure water, press same method lixiviate and cross leaching juice, merge extracted twice liquid and be leaching liquor;
(5) batching: add maize cob meal and stir in 1:1 ratio in leaching liquor;
(6) dry: above-mentioned batching is dried and made compound Chinese herb extract;
(7) composite: compound Chinese herb extract, alga oligosaccharides, sulfuric acid ester polysaccharide, manna oligosacchride, vitamin E sulfuric ester are mixed in following ratio: 60 parts of compound Chinese herb extracts, 13 parts of alga oligosaccharides, 12 parts of sulfuric acid ester polysaccharide, 12 parts of manna oligosacchrides, 3 parts of vitamin E sulfuric esters;
(8) pulverize: the composite immune reinforcing agent ultramicro grinding mixing is crossed to 100 mesh sieves.
embodiment 3:
A large yellow croaker composite immune reinforcing agent, concrete preparation process is as follows:
(1) medicinal herbs pretreatment in: the impurity in each traditional Chinese medicinal material raw materials is rejected, be chopped into 2 ~ 3mm;
(2) weigh: according to the following formula for a product of gynostemma pentaphylla herbal health care cold tea, accurately weigh 15 parts of each raw material: Huang Ling, 12 parts of honeysuckles, 10 parts of thizoma curculiginises, 5 parts of SEMEN ASTRAGALI COMPLANATIs, 5 parts, cultivated land, 6 parts of glossy ganodermas, 4 parts of kuh-sengs, 5 parts of China trees, 10 parts of milk thistles, 8 parts of sweet wormwoods, 6 parts of cordate houttuynias, 5 parts of Radix Angelicae Sinensis, 4 parts of the coptiss, 3 parts of polygala roots, 2 parts of Radix Codonopsis;
(4) extract: by leaching liquor pure water in mass ratio the ratio of 50:1 join in herbal raw material, under ultrasonic wave booster action, keep slight boiling condition 30min, cross 400 order filter clothes and get juice, filter residue adds 15 times of pure water, press same method lixiviate and cross leaching juice, merge extracted twice liquid and be leaching liquor;
(5) batching: add maize cob meal and stir in 1:1 ratio in leaching liquor;
(6) dry: above-mentioned batching is dried and made compound Chinese herb extract;
(7) composite: compound Chinese herb extract, alga oligosaccharides, sulfuric acid ester polysaccharide, manna oligosacchride, vitamin E sulfuric ester are mixed in following ratio: 55 parts of compound Chinese herb extracts, 15 parts of alga oligosaccharides, 10 parts of sulfuric acid ester polysaccharide, 15 parts of manna oligosacchrides, 5 parts of vitamin E sulfuric esters;
(8) pulverize: the composite immune reinforcing agent ultramicro grinding mixing is crossed to 100 mesh sieves.
Above embodiment is only for technical scheme of the present invention is described, but not is limited; Although with reference to previous embodiment, to being had been described in detail by invention, for the person of ordinary skill of the art, the technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And to these modifications or replacement, do not make the spirit and scope of essence disengaging the present invention technical scheme required for protection of appropriate technical solution.
Claims (4)
1. a large yellow croaker composite immune reinforcing agent, is characterized in that: this immunopotentiator is that the raw material by following mass component mixes: 50～60 parts of compound Chinese herb extracts, 13～17 parts of alga oligosaccharides, 8～12 parts of sulfuric acid ester polysaccharide, 12～18 parts of manna oligosacchrides, 3～7 parts of vitamin E sulfuric esters.
2. compound Chinese herb extract according to claim 1, is characterized in that: compound Chinese herb extract is dry making after being mixed according to the ratio of 1:1 with corncob by compound Chinese herb leaching liquor.
3. compound Chinese herb leaching liquor according to claim 2, is characterized in that: the Chinese herbal medicine by following mass fraction forms: 10～20 parts of Huang Ling, 8～16 parts of honeysuckles, 8～12 parts of thizoma curculiginises, 4～6 parts of SEMEN ASTRAGALI COMPLANATIs, 4～6 parts, cultivated land, 4～8 parts of glossy ganodermas, 3～5 parts of kuh-sengs, 4～6 parts of China trees, 8～12 parts of milk thistles, 6～10 parts of sweet wormwoods, 4～8 parts of cordate houttuynias, 4～6 parts of Radix Angelicae Sinensis, 3～5 parts of the coptiss, 2～4 parts of polygala roots, 1～3 part of Radix Codonopsis.
4. large yellow croaker composite immune reinforcing agent according to claim 1, it is characterized in that realizing by following approach: compound Chinese herb extract, alga oligosaccharides, sulfuric acid ester polysaccharide, manna oligosacchride, vitamin E sulfuric ester are mixed to rear pulverizing in proportion and evenly make.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273379A (en) * | 2014-10-10 | 2015-01-14 | 福建海大饲料有限公司 | Adult fish feed of larimichthys crocea and application of adult fish feed |
| CN104304754A (en) * | 2014-10-10 | 2015-01-28 | 福建海大饲料有限公司 | Feed for larimichthys crocea larvae and application of feed |
| CN104782934A (en) * | 2015-03-23 | 2015-07-22 | 广东海大集团股份有限公司 | Feed additive for improving body shape of large yellow croaker and application of feed additive |
| CN104799099A (en) * | 2015-04-30 | 2015-07-29 | 福建省淡水水产研究所 | Laminarin additive premix feed for pseudosciaena crocea |
| CN105707514A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Complex immunity enhancing agent for colossoma brachypomum fry breeding |
| CN106551203A (en) * | 2016-12-05 | 2017-04-05 | 浙江海洋大学 | A kind of anti pathologic immunity reinforcing agent of silvery pomfret and its application |
| CN107647187A (en) * | 2017-10-10 | 2018-02-02 | 国营无为县水产养殖场 | A kind of breeding feed for improving large yellow croaker survival rate of seedling |
| CN107801867A (en) * | 2017-09-27 | 2018-03-16 | 浙江海洋大学 | A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof |
| CN108813126A (en) * | 2018-07-06 | 2018-11-16 | 广东海因特生物技术集团有限公司 | A kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application |
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| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
| CN102845634A (en) * | 2012-08-27 | 2013-01-02 | 广东海大集团股份有限公司 | Chinese herbal medicine feed additive for preventing enteritis of Litopenaeus vannamei |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102119765A (en) * | 2010-12-20 | 2011-07-13 | 宁波大学 | Immunopotentiator for large yellow croaker adults |
| CN102845634A (en) * | 2012-08-27 | 2013-01-02 | 广东海大集团股份有限公司 | Chinese herbal medicine feed additive for preventing enteritis of Litopenaeus vannamei |
| CN103053806A (en) * | 2012-12-27 | 2013-04-24 | 深圳华大基因科技有限公司 | A synbiotic feed additive, and a preparation method and applications thereof |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104273379A (en) * | 2014-10-10 | 2015-01-14 | 福建海大饲料有限公司 | Adult fish feed of larimichthys crocea and application of adult fish feed |
| CN104304754A (en) * | 2014-10-10 | 2015-01-28 | 福建海大饲料有限公司 | Feed for larimichthys crocea larvae and application of feed |
| CN104782934A (en) * | 2015-03-23 | 2015-07-22 | 广东海大集团股份有限公司 | Feed additive for improving body shape of large yellow croaker and application of feed additive |
| CN104799099A (en) * | 2015-04-30 | 2015-07-29 | 福建省淡水水产研究所 | Laminarin additive premix feed for pseudosciaena crocea |
| CN104799099B (en) * | 2015-04-30 | 2017-12-15 | 福建省淡水水产研究所 | A kind of large yellow croaker laminarin additive premix |
| CN105707514A (en) * | 2016-03-07 | 2016-06-29 | 五河县金满塘生态种养殖家庭农场 | Complex immunity enhancing agent for colossoma brachypomum fry breeding |
| CN106551203A (en) * | 2016-12-05 | 2017-04-05 | 浙江海洋大学 | A kind of anti pathologic immunity reinforcing agent of silvery pomfret and its application |
| CN107801867A (en) * | 2017-09-27 | 2018-03-16 | 浙江海洋大学 | A kind of large yellow croaker composite immune reinforcing agent and preparation method thereof |
| CN107647187A (en) * | 2017-10-10 | 2018-02-02 | 国营无为县水产养殖场 | A kind of breeding feed for improving large yellow croaker survival rate of seedling |
| CN108813126A (en) * | 2018-07-06 | 2018-11-16 | 广东海因特生物技术集团有限公司 | A kind of pseudosciaena crocea larvae composite immune reinforcing agent and its preparation method and application |
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