CN104799099A - Laminarin additive premix feed for pseudosciaena crocea - Google Patents

Laminarin additive premix feed for pseudosciaena crocea Download PDF

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CN104799099A
CN104799099A CN201510215393.2A CN201510215393A CN104799099A CN 104799099 A CN104799099 A CN 104799099A CN 201510215393 A CN201510215393 A CN 201510215393A CN 104799099 A CN104799099 A CN 104799099A
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laminarin
yellow croaker
large yellow
feed
additive premix
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CN104799099B (en
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林建斌
梁萍
朱庆国
邱曼丽
黄志坚
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FUJIAN PROV INST OF FRESHWATER FISHERY
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FUJIAN PROV INST OF FRESHWATER FISHERY
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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Abstract

The invention relates to laminarin additive premix feed for pseudosciaena crocea and belongs to the field of aquatic feed. The feed contains 6-12% by weight of laminarin. Aimed at problems of low immunity, high morbidity, high death rate and the like in existing pseudosciaena crocea culture, a premix feed additive added with laminarin is provided, so that the immunity and disease resistance of the pseudosciaena crocea can be remarkably improved, the feed efficiency is improved, nitrogen and phosphorus emission in a culture process is reduced, water pollution is alleviated, the survival rate and growth speed of the pseudosciaena crocea are increased, the feed cost is reduced, and economic benefits are increased.

Description

A kind of large yellow croaker laminarin additive premix
Technical field
The invention belongs to aquatic feeds field, be specifically related to a kind of large yellow croaker laminarin additive premix.
Background technology
Large yellow croaker ( pseudosciaena crocea) be famous-brand and high-quality fish, economic worth is very high.In recent years, large yellow croaker aquaculture developed rapidly in China's Coastal Areas, and large yellow croaker has been the maximum kind of national seawater cage culture scale and output, was that Fujian Province and even China show unique characteristics and one of best-selling seawater fish in domestic market.At present, defined in China the large yellow croaker aquaculture industry that year nursery amount surpasses 1,000,000,000 tails, nearly 120,000 tons of cultured output (2013), the output value more than 70 hundred million yuan.But large yellow croaker is after China propagates artificially successfully at present, there is kind of a matter degeneration, immunity reduces, anti-stress ability is poor, culture diseases takes place frequently, cultivate the problems such as survival rate is lower, mariculture area is seriously polluted, strengthen large yellow croaker immunocompetence, reduce M & M in breeding process, become and reduce cultivation risk, improve culturing economic income, ensure the key that large yellow croaker aquaculture continues, develops in a healthy way.
China's marine polysaccharide is wide in variety, abundance, and it can not only improve body's immunity, and has multiple biologically active.Algal polysaccharides refers to various polymeric carbohydrates contained in marine alga, comprises the sulfated polysaccharide in alginic acid, fucoidin, green alga, the agar-agar in red algae, carragheen etc.; There is important function improving in the specificity of animal body and non-specific immunity, there is antifatigue, immunological regulation, the biologically active such as anti-ageing, antitumor, antiviral, and have no side effect, there is great Development volue.Sea-tangle ( laminaria japonica) be the important component part of marine living resources, the polysaccharose substance structure be rich in, distinct, there is multiple biologically active.Laminarin has eliminates free radical and antioxidation, has substantial worth as additive.
summary of the invention:
The object of the invention is the problems such as low for the immunity existed in existing large yellow croaker cultivation, the incidence of disease is high, the death rate is high, a kind of pre-mixing feed additive adding laminarin is provided, immunity and the disease resistance of large yellow croaker can be significantly improved, improve feed efficiency, reduce nitrogen, phosphorus emission in breeding process, alleviate water pollution, improve survival rate, the speed of growth of large yellow croaker, reduce feed cost, increase economic efficiency.
For achieving the above object, the present invention adopts following technical scheme:
A kind of large yellow croaker laminarin additive premix, this premixed feed formula contains the composition of following percentage by weight: carnitine 0.2%-0.4%, betaine 6%-10%, laminarin 6%-12%, Choline Chloride 8%-12%, calcium dihydrogen phosphate 12%-16%, complex enzyme 2%-3%, B B-complex 8%-12%, composite mineral salt 20%, other carrier 10%-40%.
Wherein B B-complex per kilogram can contain: VA 2,200,000 IU, VD 3620000 IU, VE60g, VK 33200mg, VB 12050mg, VB 23500 mg, VB 67800mg, VB 1215mg, biotin 450mg, nicotinic acid 10.5g, folic acid 500mg, calcium pantothenate 4500mg, inositol 60g, VC 120g; The rest is corn protein powder.
Composite mineral salt is made up of following salt, and the percentage by weight of its each component is: manganese sulfate 0.35%, zinc sulfate 2.25%, copper sulphate 0.16%, ferrous sulfate 1.75%, sodium selenite 0.01%, cobalt chloride 0.03%, KI 0.02%, bentonite 95.43%.
Other carrier is one or more in fish meal, bean cake powder, wheat flour.
Raw materials usedly in feed of the present invention be commercially available prod, B B-complex, composite mineral salt chemical agent used is feed grade.Used enzyme preparation is phytase and complex enzyme (comprising protease, lipase, amylase etc.).
The present invention's beneficial effect compared with prior art:
(1) sea-tangle is containing 3 kinds of polysaccharide: algin, fucoidin, laminaran.Algin and fucoidin are the fillers of cell membrane; Laminaran is present in cytoplasm.Algin refers generally to alginic acid salt, and the content in sea-tangle is very abundant, is approximately 19.7%, is be the block copolymer that monomer is formed by α-Isosorbide-5-Nitrae-L-guluronic acid and β-Isosorbide-5-Nitrae-D-MANNOSE aldehydic acid, not containing protein.The content of fucoidin in sea-tangle is about 2.46%, and main component is α-L-fucose 4-sulfuric ester polymer.The glucan that laminaran is made up of glucose, primarily of 1,3 glycosidic bonds are formed by connecting.Laminarin can improve and enhanced machine body immunity function.Research shows, laminarin has obvious antitumor action, the amynologic mechanism of its antitumor action, except itself direct inhibition tumor cell growth, also by enhanced machine body immunity function, and the growth diffusion of inhibition tumor cell.Laminarin energy activating macrophage, makes it to have CDCC, thus inhibition tumor cell is bred and kills tumour cell.So the pre-mixing feed additive adding laminarin can significantly improve immunity and the premunition of large yellow croaker;
(2) significantly improve large yellow croaker cultivation survival rate, improve feed efficiency, reduce aquaculture cost;
(3) use in large yellow croaker feed as feed addictive, addition is 4.5%-5.0%, wherein laminarin accounting 6%-12%, is converted to addition in compound fish bait used specially for pseudosciaena crocea and is only 0.27%-0.60%;
(4) the present invention to feed palatability without any harmful effect;
(5) reasonable employment of fish green additive, alternative antibiotic and the chemistry that some are harmful to urge long agent, promote digesting and assimilating of nutriment, promote fish growth, disease preventing and treating, reduce and eliminate harmful substance remaining at fish body.With the addition of the green additive such as betaine, enzyme preparation in feed of the present invention, the primary efficacy of betaine has food calling effect, can promote ingesting of large yellow croaker, improves the speed of growth of large yellow croaker, reduces the waste of feed; Can fat metabolism be promoted simultaneously, suppress fatty liver.Enzyme preparation can reduce large yellow croaker fish body alimentary canal chyme viscosity, improve nutrient digestion absorptivity, improve the utilization rate of phytate phosphorus, eliminate ANFs, activate the secretion of endogenous enzymes, improve large yellow croaker survival rate and the speed of growth, and nontoxic, harmless, noresidue, and the excretion of nitrogen, phosphorus in large yellow croaker ight soil can be reduced significantly, and alleviate the pollution to breeding water body, great to protection breeding ecological Significance for Environment;
(6) the present invention is used raw materials enjoy stable sources, moderate cost, production technology is simple.
detailed description of the invention:
The object reached to make technological means of the present invention, feature and using method and effect are easy to clear, below in conjunction with specific embodiment, set forth the present invention further, but the present invention are not limited only to this.
embodiment one:
A kind of large yellow croaker laminarin additive premix, this formula contains the composition of following percentage by weight: carnitine 0.4%, betaine 10%, laminarin 12%, Choline Chloride 12%, calcium dihydrogen phosphate 16%, complex enzyme 3%, B B-complex 12%, composite mineral salt 20%, other carrier 14.6%.
Wherein B B-complex per kilogram can contain: VA 2,200,000 IU, VD 3620000 IU, VE60g, VK 33200mg, VB 12050mg, VB 23500 mg, VB 67800mg, VB 1215mg, biotin 450mg, nicotinic acid 10.5g, folic acid 500mg, calcium pantothenate 4500mg, inositol 60g, VC 120g; The rest is corn protein powder.
Composite mineral salt is made up of following salt, and the percentage by weight of its each component is: manganese sulfate 0.35%, zinc sulfate 2.25%, copper sulphate 0.16%, ferrous sulfate 1.75%, sodium selenite 0.01%, cobalt chloride 0.03%, KI 0.02%, bentonite 95.43%.
Other carrier is fish meal.
In conventional manner each raw material components is mixed in specific proportions, be prepared from large yellow croaker premixed feed.
This formula can improve immunity and the premunition of larval Pseudosciaena crocea, thus improves survival rate, the feed efficiency of large yellow croaker, reduces feed cost, improves fanning economics.
embodiment two:
A kind of large yellow croaker laminarin additive premix, this formula contains the composition of following percentage by weight: carnitine 0.3%, betaine 8%, laminarin 10%, Choline Chloride 10%, calcium dihydrogen phosphate 14%, complex enzyme 3%, B B-complex 10%, composite mineral salt 20%, other carrier 24.7%.
Wherein B B-complex per kilogram can contain: VA 2,200,000 IU, VD 3620000 IU, VE60g, VK 33200mg, VB 12050mg, VB 23500 mg, VB 67800mg, VB 1215mg, biotin 450mg, nicotinic acid 10.5g, folic acid 500mg, calcium pantothenate 4500mg, inositol 60g, VC 120g; The rest is corn protein powder.
Composite mineral salt is made up of following salt, and the percentage by weight of its each component is: manganese sulfate 0.35%, zinc sulfate 2.25%, copper sulphate 0.16%, ferrous sulfate 1.75%, sodium selenite 0.01%, cobalt chloride 0.03%, KI 0.02%, bentonite 95.43%.
Other carrier is bean cake powder.
In conventional manner each raw material components is mixed in specific proportions, be prepared from large yellow croaker premixed feed.
This formula can improve immunity and the premunition of large yellow croaker juvenile fish, thus improves survival rate, the feed efficiency of large yellow croaker, reduces feed cost, improves fanning economics.
embodiment three:
A kind of large yellow croaker laminarin additive premix, this formula contains the composition of following percentage by weight: carnitine 0.2%, betaine 6%, laminarin 6%, Choline Chloride 8%, calcium dihydrogen phosphate 12%, complex enzyme 2%, B B-complex 8%, composite mineral salt 20%, other carrier 37.8%.
Wherein B B-complex per kilogram can contain: VA 2,200,000 IU, VD 3620000 IU, VE60g, VK 33200mg, VB 12050mg, VB 23500 mg, VB 67800mg, VB 1215mg, biotin 450mg, nicotinic acid 10.5g, folic acid 500mg, calcium pantothenate 4500mg, inositol 60g, VC 120g; The rest is corn protein powder.
Composite mineral salt is made up of following salt, and the percentage by weight of its each component is: manganese sulfate 0.35%, zinc sulfate 2.25%, copper sulphate 0.16%, ferrous sulfate 1.75%, sodium selenite 0.01%, cobalt chloride 0.03%, KI 0.02%, bentonite 95.43%.
Other carrier is wheat flour.
In conventional manner each raw material components is mixed in specific proportions, be prepared from large yellow croaker premixed feed.
This formula can improve immunity and the premunition of large yellow croaker adult fish, thus improves survival rate, the feed efficiency of large yellow croaker, reduces feed cost, improves fanning economics.
use-case:
1, laminarin is on large yellow croaker growth and the impact of immunity
Test and carry out in the rich plant producing Co., Ltd of flooding of Ningde City, adopt fiberglass drum.Drum internal diameter 150cm, height 80cm, the depth of water, about 60cm, keeps miniflow water state.3 process are established in test altogether: (laminarin is accounting 10% in premix to be added with the mixed feed group of 5% laminarin premix, be converted to addition 0.5% in mixed feed), be added with mixed feed group (laminarin is accounting 10% in premix, is converted to addition 1.0% in mixed feed), the control group of 10% laminarin premix.3 repetitions are established in each process, and large yellow croaker juvenile fish 80 tail (specification body long 16.1 cm, average weight 88.30g/tail) put in a suitable place to breed by each drum.After the raising of 32d, be added with 5% laminarin premix group, be added with 10% laminarin premix group rate of body weight gain than contrast component you can well imagine high by 26.77%, 16.23%; Be added with 5% laminarin premix group, be added with 10% laminarin premix group feed efficiency than contrast component you can well imagine high by 9.66%, 4.61%.Add test group and control group muscles of Pseudosciaena crocea moisture, crude protein, crude fat, the crude ash content difference remarkable (p>0.05) of laminarin.
As can be seen from Table 1, the large yellow croaker immunity of adding laminarin is all significantly increased than control group, immunocompetence (alkaline phosphatase AKP, lysozyme LZM, total number born T-SOD) increase (p<0.05) more obvious than control group.
The large yellow croaker gaining effect and the immune effect that are added with 5% laminarin premix group are better than being added with 10% laminarin premix group.
table 1 laminarin is on the impact of large yellow croaker immunity
Note: in table, data are mean+SD; In same row alphabetical difference shown in subscript represent significant difference ( p<0.05).
2, large yellow croaker laminarin additive premix Demonstration Application
In early October, 2014 selects the test of large yellow croaker functional collaboration diet and Demonstration And Extension work carrying out adding laminarin premixed feed in the Luo Chun village sea area cage culture of Jia Yang township of Fuding City.Test is divided into 2 groups: test group is thrown something and fed laminarin additive premix addition 5%(wherein laminarin content 10% in premix, be converted to addition 0.5% in mixed feed) large yellow croaker feed, if 2 net cages, specification is respectively 6.6m × 3.3m × 3.0m and 6.6m × 6.6m × 3.0m, puts large yellow croaker 2010 tail of average weight 51 g/ tail, large yellow croaker 4515 tail of 63g/ tail respectively in a suitable place to breed; Control group is thrown something and fed large yellow croaker basal feed, if 1 net cage, specification is 6.6m × 3.3m × 3.0m, puts large yellow croaker 2000 tail of average weight 53g/ tail in a suitable place to breed.Through the raising of 63d, take a sample test through scene, test group average body weighs 147.7 g/ tails, the long 19.3cm of average body, as calculated feed coefficient 1.38; Control group average body weighs 119.0g/ tail, the long 19.0cm of average body, as calculated feed coefficient 1.61.Use the test group of laminarin additive to close feed efficiency than contrast assembly and improve 16.26%, survival rate improves 6.03%.
In sum, a kind of large yellow croaker laminarin additive premix, can significantly improve immunity and the premunition of large yellow croaker, improves the large yellow croaker speed of growth, cultivation survival rate and feed efficiency, reduces feed cost; Can reduce the discharge capacity of nitrogen, phosphorus in breeding process, alleviate the pollution to water quality, social benefit, economic benefit and ecological benefits are remarkable.

Claims (5)

1. a large yellow croaker laminarin additive premix, is characterized in that, this feed formula contains the laminarin that percentage by weight is 6%-12%.
2. a kind of large yellow croaker laminarin additive premix according to claim 1, it is characterized in that, this premixed feed formula contains the composition of following percentage by weight: carnitine 0.2%-0.4%, betaine 6%-10%, laminarin 6%-12%, Choline Chloride 8%-12%, calcium dihydrogen phosphate 12%-16%, complex enzyme 2%-3%, B B-complex 8%-12%, composite mineral salt 20%, other carrier 10%-40%.
3. a kind of large yellow croaker laminarin additive premix according to claim 2, it is characterized in that, described B B-complex, its per kilogram can contain: VA 2,200,000 IU, VD 3620000 IU, VE60g, VK 33200mg, VB 12050mg, VB 23500 mg, VB 67800mg, VB 1215mg, biotin 450mg, nicotinic acid 10.5g, folic acid 500mg, calcium pantothenate 4500mg, inositol 60g, VC 120g; The rest is corn protein powder.
4. a kind of large yellow croaker laminarin additive premix according to claim 2, it is characterized in that, described composite mineral salt is specifically made up of following salt, and its percentage by weight is: manganese sulfate 0.35%, zinc sulfate 2.25%, copper sulphate 0.16%, ferrous sulfate 1.75%, sodium selenite 0.01%, cobalt chloride 0.03%, KI 0.02%, bentonite 95.43%.
5. a kind of large yellow croaker laminarin additive premix according to claim 2, is characterized in that, other described carrier is one or more in fish meal, bean cake powder, wheat flour.
CN201510215393.2A 2015-04-30 2015-04-30 A kind of large yellow croaker laminarin additive premix Expired - Fee Related CN104799099B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105124323A (en) * 2015-10-13 2015-12-09 威海光洋生物科技有限公司 Complex immunoenhancer for prawns
CN108185126A (en) * 2018-03-01 2018-06-22 福建省淡水水产研究所 A kind of half pierces thick lip carp compound feed and preparation method thereof
CN111393535A (en) * 2020-03-30 2020-07-10 福建大昌生物科技实业有限公司 Extraction method of laminarin and application of laminarin in aquatic feed
CN114176176A (en) * 2021-11-29 2022-03-15 无锡泛宁生物科技有限公司 Feed additive for aquatic animals and preparation method thereof

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105124323A (en) * 2015-10-13 2015-12-09 威海光洋生物科技有限公司 Complex immunoenhancer for prawns
CN108185126A (en) * 2018-03-01 2018-06-22 福建省淡水水产研究所 A kind of half pierces thick lip carp compound feed and preparation method thereof
CN111393535A (en) * 2020-03-30 2020-07-10 福建大昌生物科技实业有限公司 Extraction method of laminarin and application of laminarin in aquatic feed
CN114176176A (en) * 2021-11-29 2022-03-15 无锡泛宁生物科技有限公司 Feed additive for aquatic animals and preparation method thereof

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