CN101579057B - Method for preparing sea cucumber compound feed with additional Enteromorpha - Google Patents
Method for preparing sea cucumber compound feed with additional Enteromorpha Download PDFInfo
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- CN101579057B CN101579057B CN2009100162039A CN200910016203A CN101579057B CN 101579057 B CN101579057 B CN 101579057B CN 2009100162039 A CN2009100162039 A CN 2009100162039A CN 200910016203 A CN200910016203 A CN 200910016203A CN 101579057 B CN101579057 B CN 101579057B
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- enteromorpha
- sea cucumber
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a method for preparing sea cucumber compound feed with additional Enteromorpha. In the method, sea cucumber compound feed is added and mixed with dry Enteromorpha powder to form the sea cucumber compound feed with additional Enteromorpha, the coefficient of variation of the obtained sea cucumber compound feed with additional Enteromorpha after being fully mixed is less than or equal to 10 percent, and the obtained sea cucumber compound feed with additional Enteromorpha are made into grains with a diameter of 3 millimeters by using 2 percent sodium alginate as a binder. The added dry Enteromorpha powder accounts for 30 percent of the weight of the sea cucumber compound feed with additional Enteromorpha. The sea cucumber compound feed with additional Enteromorpha comprises the following ingredients by weight percentage: 10 percent of fish meal, 5 percent of Sargassum thunbergii powder, 20 percent of vinasse, 15 percent of sea mud, 10 percent of bean pulp, 30 percent of Enteromorpha powder, 8 percent of bran, 1 percent of vitamin mix and 1 percent of inorganic slat mix. In the sea cucumber compound feed with additional Enteromorpha prepared by the formulation,the Enteromorpha powder used to replace kelp powder can reduce the cost of feed prepared by the conventional formulation (not using the Enteromorpha powder) and avoids influencing the growth of Apostichopus japonicus Selenka; and the survival rate of the Apostichopus japonicus Selenka is improved remarkably (P is less than 0.05).
Description
Technical field:
The invention belongs to the preparation technique of sea cucumber compound feed, is a kind of method of adding Enteromorpha preparation sea cucumber compound feed.
Background technology:
Sea cucumber belongs to Echinodermata (Echinoderma ta), Holothuroidea (Holothurioidea).Holothuroidea is the guiding principle that economic worth is arranged in the peronium animal door most, and kind is a lot, kind surplus the whole world has 1100.There is kind more than 100 in China marine site, wherein edible have 20 surplus kind.Break through sea cucumber artificial seed raising technology the eighties; Seed rearing reaches the scale level; The fast development of holothruian cultures scale, the breed scale constantly enlarges, and the growth of overrunning of holothruian cultures has caused the active demand to mixed feed; The price surge of mouse tail algae, sargassum and Zostera marina etc., and supply falls short of demand.
Enteromorpha is a kind of large-scale green alga; Be commonly called as tongue bar, green laver etc.; Be the algae of Chlorophyta Ulvales Ulvaceae Enteromorpha, common have chance with pipe Enteromorpha (Enteromorpha linza), flat Enteromorpha (Enteromorpha compressa), bar Enteromorpha (Enteromorpha clathra t), intestines Enteromorpha (Enteromorpha intestinalis) etc.Enteromorpha is the natural wild green alga in the beach of coastal waters, and its natural propagation ability is strong especially, and output is huge.Enteromorpha is rich in carbohydrate, protein, crude fibre and mineral matter, also contains fat and vitamin simultaneously.In Enteromorpha protein, the amino acid A wide selection of colours and designs, essential amino acids content is higher, and wherein the limiting amino acid of edge pipe Enteromorpha is a lysine, and AAS is 79; The limiting amino acid of bar Enteromorpha is a methionine, and AAS is 80.During the aliphatic acid of Enteromorpha was formed, the content of polyunsaturated fatty acid, monounsaturated fatty acids and saturated fatty acid was for being respectively 50.5%, 12.7% and 36.8%, the odd carbon fatty acid comprising nearly 4%.Therefore Enteromorpha is high protein, high dietary-fiber, low fat, low-yield, and is rich in the raw material of the natural desirable nutraceutical of minerals and vitamins.
The evidence of feed research institute of the Chinese Academy of Agricultural Sciences, the smart powder of the Enteromorpha of interpolation 15% is raised laying hen in feed, can make cholesterol reduction by 21.6% in the egg.High and female etc. discovered, in egg feedstuff, adds seawood meal, and content of cholesterol significantly reduces in the yolk, compares significant difference with control group.Gu Haiyuan etc. add seawood meal in egg feedstuff, the result finds that along with the prolongation of test period, yolk color is deepened gradually, and the content of iodine increases in the egg.In sea cucumber compound feed, the application of adding green algas such as sea-tangle powder, mouse tail algae powder is comparatively general, but the application of Enteromorpha powder in sea cucumber do not appear in the newspapers.
Summary of the invention:
The objective of the invention is to utilize this natural wild green alga of Enteromorpha in the beach of coastal waters, substitute the sea-tangle powder as sea cucumber compound feed, reduce production costs, and also do not influence the growth of sea cucumber with Enteromorpha dry powder.
The present invention accomplishes by following operating technology: it is to utilize Enteromorpha dry powder to make an addition in the sea cucumber compound feed to mix, and fully behind the mixing, the coefficient of variation≤10% is made adhesive with the sodium alginate of concentration 2%, granulates, and particle is φ 3mm.
Enteromorpha dry powder addition (weight ratio) is: sea cucumber compound feed 30%.
Sea cucumber compound feed (weight ratio) is: by fish meal 10%, mouse tail algae powder 5%, vinasse 20%, ooze 15%, dregs of beans 10%, Enteromorpha powder 30%, wheat bran 8%, vitamin mixtures 1%, inorganic salt mixt 1%.
The contrast of the present invention and prior art has following characteristics:
1, sea cucumber compound feed substitutes the sea-tangle powder with Enteromorpha dry powder, does not influence the growth of sea cucumber.
2, the sea cucumber survival rate improves, and survival rate is 98 ± 2.4%.
3, the sea cucumber compound feed cost reduces.
The specific embodiment:
Usually the use amount at sea cucumber compound feed sea-tangle powder is 30%; The price of sea-tangle powder is 25000 yuan/ton; And Enteromorpha is wild green alga, is processed into 5000/ ton of Enteromorpha powder price, in sea cucumber compound feed, uses the Enteromorpha powder can reduce the cost of sea cucumber compound feed greatly.
The present invention's technology, the mixed feed that utilizes fish meal, dregs of beans, vinasse, wheat bran etc. to process substitutes the sea-tangle powder with Enteromorpha dry powder, test manufacture 500kg, the sea cucumber effect of feeding is good.
Be described in detail compound method of the present invention through embodiment below.
The present invention accomplishes by following operating technology: select for use fish meal 10%, dregs of beans 10%, vinasse 20%, wheat bran 8%, mouse tail algae 5%, Enteromorpha dry powder 30%, vitamin mixtures 1%, mineral mixture 1% etc. fully to mix.
The preliminary treatment of Enteromorpha raw material: fresh Enteromorpha, seawater flushing drains, and Enteromorpha dry powder is processed in 60 degree oven dry, and Analysis of Nutritional is following, sees table 1:
Table 1 Enteromorpha dry powder Analysis of Nutritional
Project | Content |
Moisture | 6.74% |
Ash content | 24.98% |
Crude protein | 10.11% |
Crude fat | 1.42% |
Crude fibre | 5.96% |
Polysaccharide | 39.26% |
Salinity | 7.48% |
Calcium | 11248.59 |
Magnesium | 12432.98 |
Phosphorus | 522.92 |
Nitrogen | 1.82% |
Chlorine | 7.48% |
Iron | 241.12mg/Kg |
Zinc | 15.07mg/Kg |
Copper | 14.63mg/Kg |
Iodine | Do not detect |
Selenium | 0.72 |
Chromium | 0.92 |
Organo-arsenic | 5.41 |
Inorganic arsenic | 0.39 |
Cadmium | 0.52 |
Plumbous | 0.12 |
Mercury | Do not detect |
The screening process of optimum implementation:
The suitable addition of Enteromorpha dry powder: adopt respectively and add 0 (contrast), 10%, 20%, 30% Enteromorpha dry powder substitutes the sea-tangle powder, processes sea cucumber compound feed, feed formula such as table 2; Feed 6 weeks of sea cucumber, observe its influence, see table 3 ingesting, grow, surviving.Control group is conventional formulation commonly used.
Table 2: each organizes feed formula
Composition | 1 (contrast) | 2 | 3 | 4 |
Fish meal | ?10 | 10 | 10 | 10 |
Mouse tail algae powder | ?5 | 5 | 5 | 5 |
Vinasse | ?20 | 20 | 20 | 20 |
Ooze | ?15 | 15 | 15 | 15 |
Dregs of beans | ?10 | 10 | 10 | 10 |
The sea-tangle powder | ?30 | 20 | 10 | 0 |
The Enteromorpha powder | ?0 | 10 | 20 | 30 |
Wheat bran | ?8 | 8 | 8 | 8 |
Vitamin mixtures 1 | ?1 | 1 | 1 | 1 |
Inorganic salt mixt 2 | ?1 | 1 | 1 | 1 |
1Vitamin mixtures (mg or g/kg feed): thiamine, 25mg; Riboflavin, 45mg; Puridoxine hydrochloride, 20mg; Cobalamin, 0.1mg; Prokeyvit, 10mg; Inositol, 800mg; Pantothenic acid, 60mg; Nicotinic acid, 200mg; Folic acid, 20mg; Biotin, 1.20mg; Vitamin A, 32mg; Vitamin D, 5mg; Vitamin E, 120mg; Inferior powder 18.67g.
2Inorganic salt mixt (mg or g/kg feed): sodium fluoride, 2mg; KI, 0.8mg; Cobalt chloride, 50mg; Copper sulphate, 10mg; Ferric sulfate, 80mg; Zinc sulfate, 50mg; Magnesium sulfate, 1200mg; Calcium dihydrogen phosphate, 3000mg; Common salt, 100mg; Zeolite powder, 15.45g.
The influence that table 3 Enteromorpha dry powder is ingested, grown turbot
Group | 1 (contrast) | 2 Enteromorpha dry powder (10%) | 3 Enteromorpha dry powder (20%) | 4 Enteromorpha dry powder (30%) |
Rate of body weight gain (%) | 84±1.5 a | 86±2.3 a | 84±1.6 a | 87±2.6 a |
Survival rate (%) | 90±2.5 a | 90±2.0 a | 95±3.0 b | 98±2.4 b |
Annotate: samely list angle different English subscript letter representation there were significant differences (P<0.05).
Can find out the rate of body weight gain (P>0.05) that does not influence sea cucumber when the alternative sea-tangle powder of feed interpolation 30%, 20% and 10% Enteromorpha dry powder from table 2, reduce cost the purpose that turns waste into wealth thereby reach; Feed adds the survival rate (P<0.05) that 30%, 20% Enteromorpha can significantly improve sea cucumber.
Claims (1)
1. a method of adding Enteromorpha preparation sea cucumber compound feed is characterized in that it is to utilize Enteromorpha dry powder to make an addition in the sea cucumber compound feed to mix, fully behind the mixing; The coefficient of variation≤10%; Sodium alginate with concentration 2% is made adhesive, granulates, and particle is ф 3mm;
Described Enteromorpha dry powder, its addition percentage by weight is: account for 30% of sea cucumber compound feed;
Described sea cucumber compound feed; Its food ingredient and consumption, percentage by weight is: fish meal 10%, mouse tail algae powder 5%, vinasse 20%, ooze 15%, dregs of beans 10%, Enteromorpha powder 30%, wheat bran 8%, vitamin mixtures 1%, inorganic salt mixt 1%.
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Cited By (1)
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CN103271250A (en) * | 2013-06-27 | 2013-09-04 | 江苏海辰科技集团有限公司 | Enteromorpha hard pellet feed for holothurian culture |
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CN101791071A (en) * | 2010-04-09 | 2010-08-04 | 大连理工大学 | Environment-friendly sea cucumber micelle feeds and preparation method thereof |
CN101926422A (en) * | 2010-07-24 | 2010-12-29 | 青岛农业大学 | Dorking compound feed containing Enteromorpha |
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CN103082152A (en) * | 2013-02-07 | 2013-05-08 | 大连海洋大学 | Feed for reducing lead content of sea cucumber |
CN104738325A (en) * | 2013-12-26 | 2015-07-01 | 王宝燕 | Feed for juvenile sea cucumber |
CN104738326A (en) * | 2013-12-26 | 2015-07-01 | 王宝燕 | Glycosidase-containing sea cucumber feed |
CN104431418A (en) * | 2014-11-05 | 2015-03-25 | 中国水产科学研究院黄海水产研究所 | Stichopus japonicus complete formula feed |
CN105639209B (en) * | 2016-03-21 | 2018-07-06 | 中国水产科学研究院黄海水产研究所 | A kind of enteromorpha stichopus japonicus biological feedstuff and preparation method thereof |
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Cited By (1)
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CN103271250A (en) * | 2013-06-27 | 2013-09-04 | 江苏海辰科技集团有限公司 | Enteromorpha hard pellet feed for holothurian culture |
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